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Pesquisa : E02.875.800.984.500 [Categoria DeCS]
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[PMID]:28865223
[Au] Autor:Palubinskas G; Zilaitis V; Antanaitis R
[Ti] Título:Improvement of dairy cow embryo yield with low level laser irradiation.
[So] Source:Pol J Vet Sci;20(2):307-312, 2017 Mar 01.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The goal of this study is to estimate the effects of low-level laser irradiation (LLLI) on the superovulatory response according to the number of corpora lutea (CL), follicles (F) and the embryo yield. In recent years, while searching for new, more efficient and organic methods to improve superovulatory response and embryo yield with respect to the conventional methods, low-level laser irradiation (LLLI) is a more sensitive and less costly technology that can be used to improve animal reproduction, namely, artificial insemination and the embryo production system. The dairy-cow donors were treated for superovulation with Pluset®, at any time during the oestrus cycle, and the total dose per donor was 700 IU. The first group of the donors (n=25), test group (TG), was irradiated on the sacroiliac area for 180 seconds per day, from the 1st to 11th superovulatory treatment (ST) days in a row, with LLLI in the 870-970-nm wavelength, 65.93 J/cm dose, frequencies in the 20-2000 Hz range and pulse durations commonly in the range of about 1 second. For the second control group (CG) (n=25), the ST was performed without LLLI. After the ST, The mean number of CL in the right side ovaries in the TG was 25.43% (p<0.05) greater than in those of the CG. The number of total recovered and transferable embryos was greater in the TG compared with the CG by 28.97% (p<0.05) and 15.8% (p>0.05), respectively. With respect to conventional methods, LLLI can be used to improve the superovulatory response and embryo yield as a supplementary environment and animal-friendly method of treatment.
[Mh] Termos MeSH primário: Bovinos/embriologia
Transferência Embrionária/veterinária
Lasers
Ovário/efeitos da radiação
Superovulação/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Embrião de Mamíferos
Feminino
Hormônio Foliculoestimulante/administração & dosagem
Hormônio Foliculoestimulante/farmacologia
Hormônio Luteinizante/administração & dosagem
Hormônio Luteinizante/farmacologia
Indução da Ovulação/veterinária
Superovulação/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9002-67-9 (Luteinizing Hormone); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE


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[PMID]:28438565
[Au] Autor:Taiyeb AM; Muhsen-Alanssari SA; Dees WL; Hiney J; Kjelland ME; Kraemer DC; Ridha-Albarzanchi MT
[Ad] Endereço:College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4466, USA; Barz IVF Center for Embryo Research and Infertility Treatment, Koya Street, Brayati, Erbil 44001, Kurdistan, Iraq. Electronic address: amtaiyeb@aggienetwork.com.
[Ti] Título:Improvements in oocyte competence in superovulated mice following treatment with cilostazol: Ovulation of immature oocytes with high developmental rates.
[So] Source:Biochem Pharmacol;137:81-92, 2017 Aug 01.
[Is] ISSN:1873-2968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Exogenous administration of superovulatory hormones negatively affects oocyte competence in mammals. Phosphodiesterase 3A inhibitors were found to improve competence of oocytes matured in vitro in several species, including humans. This study was therefore designed to define oocyte maturation synchronization and competence, in vivo, using superovulated mice treated with cilostazol, a selective phosphodiesterase 3A inhibitor. Swiss Webster mice were superovulated and treated orally with 7.5mg cilostazol once or twice to result in ovulation of immature oocytes at the metaphase I (MI) or germinal vesicle (GV) stage, respectively. Control immature oocytes were recovered from preovulatory follicles of superovulated mice not treated with cilostazol. Treated GV oocytes had significantly higher rates of synchronized and advanced chromatin configuration and cortical granule distribution than did control GV oocytes. Treated GV oocytes had a moderate increase in cAMP levels and consequently higher rates of meiotic maturation, IVF, and blastocyst formation than did control GV oocytes (P<0.0001). Treated MI oocytes had higher rates of normal spindles and chromosomes aligned at the metaphase plate than did control MI oocytes (P<0.003). Treated mice ovulating MI oocytes produced litter sizes larger than those observed in control mice ovulating mature oocytes (P<0.002). This study reveals that synchronization of oocyte maturation in superovulated mice improves oocyte development and competence. The capability of cilostazol, a clinically approved medication, to improve mouse oocyte competence suggests the potential benefit of including this compound in ovarian hyperstimulation programs to improve in vitro fertilization outcomes in infertile women.
[Mh] Termos MeSH primário: Oócitos/efeitos dos fármacos
Oócitos/crescimento & desenvolvimento
Superovulação/efeitos dos fármacos
Superovulação/fisiologia
Tetrazóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Desenvolvimento Embrionário/efeitos dos fármacos
Desenvolvimento Embrionário/fisiologia
Feminino
Seres Humanos
Masculino
Camundongos
Ovulação/efeitos dos fármacos
Ovulação/fisiologia
Inibidores da Fosfodiesterase 3/farmacologia
Projetos Piloto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphodiesterase 3 Inhibitors); 0 (Tetrazoles); N7Z035406B (cilostazol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


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[PMID]:28238490
[Au] Autor:Morbeck DE; Baumann NA; Oglesbee D
[Ad] Endereço:Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, Minnesota; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. Electronic address: deanmorbeckphd@gmail.com.
[Ti] Título:Composition of single-step media used for human embryo culture.
[So] Source:Fertil Steril;107(4):1055-1060.e1, 2017 Apr.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine compositions of commercial single-step culture media and test with a murine model whether differences in composition are biologically relevant. DESIGN: Experimental laboratory study. SETTING: University-based laboratory. ANIMAL(S): Inbred female mice were superovulated and mated with outbred male mice. INTERVENTION(S): Amino acid, organic acid, and ions content were determined for single-step culture media: CSC, Global, G-TL, and 1-Step. To determine whether differences in composition of these media are biologically relevant, mouse one-cell embryos were cultured for 96 hours in each culture media at 5% and 20% oxygen in a time-lapse incubator. MAIN OUTCOME MEASURE(S): Compositions of four culture media were analyzed for concentrations of 30 amino acids, organic acids, and ions. Blastocysts at 96 hours of culture and cell cycle timings were calculated, and experiments were repeated in triplicate. RESULT(S): Of the more than 30 analytes, concentrations of glucose, lactate, pyruvate, amino acids, phosphate, calcium, and magnesium varied in concentrations. Mouse embryos were differentially affected by oxygen in G-TL and 1-Step. CONCLUSION(S): Four single-step culture media have compositions that vary notably in pyruvate, lactate, and amino acids. Blastocyst development was affected by culture media and its interaction with oxygen concentration.
[Mh] Termos MeSH primário: Blastocisto/fisiologia
Meios de Cultura/química
Técnicas de Cultura Embrionária
Indução da Ovulação/métodos
Superovulação
[Mh] Termos MeSH secundário: Aminoácidos/análise
Animais
Blastocisto/metabolismo
Hipóxia Celular
Microambiente Celular
Meios de Cultura/metabolismo
Desenvolvimento Embrionário
Feminino
Ácido Láctico/análise
Masculino
Camundongos
Oxigênio/metabolismo
Ácido Pirúvico/análise
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Culture Media); 33X04XA5AT (Lactic Acid); 8558G7RUTR (Pyruvic Acid); S88TT14065 (Oxygen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE


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[PMID]:28237339
[Au] Autor:Mollo MR; Monteiro PL; Surjus RS; Martins AC; Ramos AF; Mourão GB; Carrijo LH; Lopes G; Rumpf R; Wiltbank MC; Sartori R
[Ad] Endereço:National Water Agency (ANA), Brasília, Federal District, Brazil.
[Ti] Título:Embryo production in heifers with low or high dry matter intake submitted to superovulation.
[So] Source:Theriogenology;92:30-35, 2017 Apr 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the influence of feed intake on superovulatory response and embryo production of Nelore heifers. Pubertal heifers were kept in a feedlot and were submitted to the same diets, but with different levels of feed consumption: High (1.7 M; n = 20) or Low (0.7 M; n = 19) feed intake. Heifers in the 1.7 M treatment consumed 170% (2.6% of body weight [BW] in dry matter) and the 0.7 M heifers ate 70% (1.1% of BW in dry matter) of a maintenance diet. After 7 wk on these diets, heifers were treated with eight decreasing doses of follicle-stimulating hormone (FSH) given every 12 h, totaling 133 mg Folltropin (Folltropin-V; Bioniche Animal Health, Canada) per heifer. Seven d after AI, heifers had their uteri flushed and embryos were recovered and graded according to the International Embryo Technology Society standards. Data were analyzed using the GLIMMIX procedure of SAS and results are presented as least-squares means ± SEM (P < 0.05). At the onset of the FSH treatment (Day 0 of the protocol), 1.7 M heifers had greater body condition score (BCS), BW and serum insulin concentrations than 0.7 M heifers (4.1 ± 0.1 vs. 3.0 ± 0.1; 462.5 ± 10.1 vs. 382.7 ± 10.4 kg; and 14.3 ± 1.7 vs. 3.5 ± 0.8 µIU/mL, respectively). The 0.7 M heifers had more follicles ≥6 mm at the time of the last FSH (Day 7; 47.9 ± 6.4 vs. 23.5 ± 4.3 follicles), related to a better follicle superstimulatory response to FSH. Similarly, 0.7 M heifers had more corpora lutea at the time of embryo collection (33.6 ± 1.4 vs. 15.7 ± 0.9) than the 1.7 M heifers, which resulted in greater number of recovered embryos and ova (9.9 ± 0.7 vs. 6.7 ± 0.6) and viable embryos (5.3 ± 0.5 vs. 3.8 ± 0.4), despite having similar proportions of viable embryos (∼62%). A negative correlation between circulating insulin and follicle superstimulatory response to FSH was observed (r = -0.68). Therefore, we conclude that high feed intake, for a long period of time, compromised the superovulatory response and embryo production potential of Bos indicus heifers possibly related to the elevation in circulating insulin.
[Mh] Termos MeSH primário: Ração Animal/análise
Bovinos/fisiologia
Dieta/veterinária
Superovulação
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Composição Corporal
Peso Corporal
Técnicas de Cultura Embrionária
Feminino
Fertilização In Vitro
Coleta de Tecidos e Órgãos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


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[PMID]:28237324
[Au] Autor:Cervantes MP; Palomino JM; Anzar M; Mapletoft RJ; Mastromonaco GF; Adams GP
[Ad] Endereço:Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
[Ti] Título:In vitro-production of embryos using immature oocytes collected transvaginally from superstimulated wood bison (Bison bison athabascae).
[So] Source:Theriogenology;92:103-110, 2017 Apr 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two experiments were done to test the hypothesis that morphologic characteristics of wood bison cumulus-oocyte complexes (COC) are reflective of the ability of the oocyte to develop to an advanced embryonic stage after in vitro maturation, fertilization and culture, and to determine the effect of prolonging the interval from the end of superstimulation treatment to oocyte collection (FSH starvation period). Experiments were done during the anovulatory season. In Experiment 1, ovarian superstimulation was induced in 10 bison with two doses of FSH given at 48 h intervals beginning at the time of follicular wave emergence. COC were collected 3 days (72 h) after the last dose of FSH by follicular aspiration and classified as compact, expanded or denuded. The COC were matured in vitro for 24 h before fertilization in vitro (Day 0). Embryo development was assessed on Days 3, 7 and 8. The blastocyst rate was 7/34, 2/10 and 0/3 in COC classified as compact, expanded and denuded, respectively; however, only compact COC resulted in embryos that reached the expanded blastocyst stage. In Experiment 2, COC were collected at either 3 or 4 days (72 or 96 h) after the last dose of FSH (n = 16 bison/group) to determine the effect of the duration of FSH starvation on oocyte competence. The COC were classified as compact good (>3 layers of cumulus cells), compact regular (1-3 layers of cumulus cells), expanded or denuded, and then matured, fertilized and cultured in vitro. Although follicles were larger (P < 0.05) in the 4-day FSH starvation group, there was no effect of starvation period on the distribution of COC morphology; overall, 112/194 (57.7%) were compact, 29/194 (26.3%) were expanded, 39/194 (20.1%) were denuded, and 14/194 (7.2%) were degenerated (P < 0.05). Similarly, there was no effect of starvation period on embryo development. Compact good COC had the highest cleavage (88%) and blastocyst rates (54%; P < 0.05), followed by compact regular COC at 73% and 25%, respectively. Expanded and denuded COC had low cleavage (40% vs. 59%, respectively) and blastocyst rates (5% vs. 8%, respectively). We conclude that morphologic characteristics of wood bison COC are reflective of the ability of the oocyte to develop into an embryo in vitro. Importantly, oocytes collected from superstimulated bison during the anovulatory season were competent to develop to the blastocyst stage following in vitro maturation, fertilization and culture.
[Mh] Termos MeSH primário: Bison/fisiologia
Técnicas de Cultura Embrionária/veterinária
Fertilização In Vitro/veterinária
Recuperação de Oócitos/veterinária
Oócitos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Hormônio Foliculoestimulante
Projetos Piloto
Superovulação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


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[PMID]:28181120
[Au] Autor:Chankitisakul V; Pitchayapipatkul J; Chuawongboon P; Rakwongrit D; Sakhong D; Boonkum W; Vongpralub T
[Ad] Endereço:Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
[Ti] Título:Comparison of three superovulation protocols with or without GnRH treatment at the time of artificial insemination on ovarian response and embryo quality in Thai native heifers.
[So] Source:Trop Anim Health Prod;49(3):633-639, 2017 Mar.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To optimize the superovulation protocol in Thai native cattle, the present research was designed to (1) compare three different protocols designed to induce superstimulation and (2) study the effect of gonadotropin-releasing hormone (GnRH) administration at insemination time (to induce ovulation) on ovarian follicular activities in terms of the number of large follicles, corpora lutea (CLs) and unovulated follicles, and the number and quality of ova/embryos recovered in Thai native heifers. Initially, the estrous cycles of animals (n = 36) at unknown stages were synchronized by two prostaglandin F (PGF ) injections at an interval of 12 days. Follicular development of heifers was randomly superstimulated with one of three different treatment protocols: treatment A-a total of 100 mg of pituitary-derived FSH (pFSH; Folltropin®-V) administered in eight decreasing doses; treatment B-a single dose of 100 mg pFSH dissolved in 30% (w/v) polyvinylpyrrolidone; or treatment C-ablation of all follicles ≥5 mm with a single dose of pFSH. All heifers received PGF 48 h after the initiation of FSH treatment to induce luteolysis from the previous cycle, and they were twice inseminated at 12 and 24 h after the onset of estrus. Heifers in each treatment were assigned to be injected or not with GnRH at the time of first insemination with frozen/thawed semen to induce ovulation. About 7 days after artificial insemination (AI), ova/embryos were collected and classified. The numbers of large follicles at the onset of estrus were not statistically significantly different; meanwhile, the maximum diameters of follicles at the time of first insemination in treatment C were smaller compared with the other treatment groups (p < 0.001). The administration of GnRH at the first insemination time resulted in a greater number of CLs and fewer unovulated follicles at the time of ova/embryo collection (p = 0.001), which subsequently resulted in a higher number of total ova/embryos recovered (p = 0.030). Among heifers treated with different superstimulation protocols, the ablation of all follicles ≥5 mm in diameter before superstimulation (treatment C) resulted in significantly higher quality of fertilized ova and transferable embryos (p = 0.001). In summary, it could be inferred that GnRH treatment improved ovarian function rather than embryo quality. Dominant follicle ablation prior to superstimulation is preferable for collecting a greater number of transferable embryos.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Hormônio Liberador de Gonadotropina/farmacologia
Inseminação Artificial/veterinária
Superovulação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Esquema de Medicação
Embrião de Mamíferos/efeitos dos fármacos
Estro/efeitos dos fármacos
Feminino
Hormônio Foliculoestimulante/administração & dosagem
Masculino
Folículo Ovariano/efeitos dos fármacos
Gravidez
Progesterona/administração & dosagem
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
33515-09-2 (Gonadotropin-Releasing Hormone); 4G7DS2Q64Y (Progesterone); 9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1243-6


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[PMID]:28131573
[Au] Autor:Parker Gaddis KL; Dikmen S; Null DJ; Cole JB; Hansen PJ
[Ad] Endereço:Department of Animal Sciences, University of Florida, Gainesville 32611. Electronic address: kristen.gaddis@cdcb.us.
[Ti] Título:Evaluation of genetic components in traits related to superovulation, in vitro fertilization, and embryo transfer in Holstein cattle.
[So] Source:J Dairy Sci;100(4):2877-2891, 2017 Apr.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objectives of this study were to estimate variance components and identify regions of the genome associated with traits related to embryo transfer in Holsteins. Reproductive technologies are used in the dairy industry to increase the reproductive rate of superior females. A drawback of these methods remains the variability of animal responses to the procedures. If some variability can be explained genetically, selection can be used to improve animal response. Data collected from a Holstein dairy farm in Florida from 2008 to 2015 included 926 superovulation records (number of structures recovered and number of good embryos), 628 in vitro fertilization records (number of oocytes collected, number of cleaved embryos, number of high- and low-quality embryos, and number of transferrable embryos), and 12,089 embryo transfer records (pregnancy success). Two methods of transformation (logarithmic and Anscombe) were applied to count variables and results were compared. Univariate animal models were fitted for each trait with the exception of pregnancy success after embryo transfer. Due to the binary nature of the latter trait, a threshold liability model was fitted that accounted for the genetic effect of both the recipient and the embryo. Both transformation methods produced similar results. Single-step genomic BLUP analyses were performed and SNP effects estimated for traits with a significant genetic component. Heritability of number of structures recovered and number of good embryos when log-transformed were 0.27 ± 0.08 and 0.15 ± 0.07, respectively. Heritability estimates from the in vitro fertilization data ranged from 0.01 ± 0.08 to 0.21 ± 0.15, but were not significantly different from zero. Recipient and embryo heritability (standard deviation) of pregnancy success after embryo transfer was 0.03 (0.01) and 0.02 (0.01), respectively. The 10-SNP window explaining the largest proportion of variance (0.37%) for total structures collected was located on chromosome 8 beginning at 55,663,248 bp. Similar regions were identified for number of good embryos, with the largest proportion of variance (0.43%) explained by a 10-SNP window on chromosome 14 beginning at 26,713,734 bp. Results indicate that there is a genetic component for some traits related to superovulation and that selection should be possible. Moreover, the genetic component for superovulation traits involves some genomic regions that are similar to those for other fertility traits currently evaluated.
[Mh] Termos MeSH primário: Transferência Embrionária/veterinária
Superovulação
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Fertilização In Vitro/veterinária
Oócitos
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


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[PMID]:27865416
[Au] Autor:Mikkola M; Taponen J
[Ad] Endereço:Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland; VikingGenetics Finland, Hollola, Finland. Electronic address: marja.mikkola@vikinggenetics.com.
[Ti] Título:Embryo yield in dairy cattle after superovulation with Folltropin or Pluset.
[So] Source:Theriogenology;88:84-88, 2017 Jan 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two commercial FSH products were compared in a retrospective study on 3990 commercial superovulations and embryo recoveries in dairy heifers and cows. In addition, the 56-day nonreturn rate of 19,400 embryos produced with these two preparations was analyzed. Embryo collections were performed during a 16-year period from donors of Holstein and Ayrshire breeds. Folltropin (Vetoquinol S.A., Lure cedex, France) group (Group F) consisted of 2592 superovulations, of which 80% were performed on heifers and 20% on cows, and Pluset (Laboratorios Calier, S.A., Barcelona, Spain) group (Group P) of 1398 treatments, of which 66% and 34% were on heifers and cows, respectively. Total number of recovered structures, number of transferable embryos, and the proportion of unfertilized ova (UFO) and degenerated embryos were analyzed. Distribution of embryos into quality grades (1-3) and developmental stages (4-9) according to the IETS classification guidelines and means for each collection were evaluated. The proportion of low-responders having fewer than five corpora lutea and yielding fewer than five embryos or ova was investigated for each treatment. Group P yielded 1.1 recovered structures more than Group F (P < 0.001). Consequently, however, the number of transferable embryos did not differ among the groups, being 7.0 and 7.1 in Groups F and P, respectively. Instead, there was an increase in the number of UFO from 2.0 in Group F to 3.0 in Group P (P < 0.001). The quality of embryos and the developmental stages were similar between the groups and there was no difference in the proportion of low-responding donors in Group F and Group P. Also, there was no difference in the nonreturn rate after transfer of embryos originating from donors superovulated with Folltropin or Pluset. It was concluded that equal numbers of transferable embryos and pregnancies can be achieved with Folltropin and Pluset.
[Mh] Termos MeSH primário: Bovinos/embriologia
Hormônio Foliculoestimulante/farmacologia
Superovulação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Transferência Embrionária
Feminino
Hormônio Foliculoestimulante/administração & dosagem
Inseminação Artificial/veterinária
Masculino
Estudos Retrospectivos
Sêmen
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9002-68-0 (Follicle Stimulating Hormone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE


  9 / 1814 MEDLINE  
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[PMID]:27853050
[Au] Autor:Hirayama H; Naito A; Fukuda S; Fujii T; Asada M; Inaba Y; Takedomi T; Kawamata M; Moriyasu S; Kageyama S
[Ad] Endereço:Animal Biotechnology Group, Animal Research Center, Hokkaido Research Organization, Hokkaido 081-0038, Japan.
[Ti] Título:Long-term changes in plasma anti-Müllerian hormone concentration and the relationship with superovulatory response in Japanese Black cattle.
[So] Source:J Reprod Dev;63(1):95-100, 2017 Feb 16.
[Is] ISSN:1348-4400
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The concentration of circulating anti-Müllerian hormone (AMH) in cattle is a useful endocrine marker for ovarian response to superovulation. Although the AMH concentration undergoes little variation throughout the estrous cycle, its long-term changes remain incompletely understood. Here, we investigated the relationship between superovulation response and plasma AMH concentration in Japanese Black cattle and the long-term changes in plasma AMH concentration of embryo donor cows and heifers. The median, 25th percentile, and 75th percentile of AMH concentrations in 222 mature animals were 0.265, 0.118, and 0.488 ng/ml, respectively. The numbers of ova/embryos, fertilized embryos, and transferable embryos in a total of 295 superovulations were significantly different among the H (AMH ≥ 0.488 ng/ml), M (AMH 0.487-0.119 ng/ml), and L (AMH ≤ 0.118 ng/ml) groups. AMH concentrations during repeated superovulation in ten donor cows were significantly decreased after the third treatment. In heifers, the highest AMH concentration was observed in individuals during 2-13 months of age, with considerable individual variability. AMH concentrations of heifers at 10 or 11 months correlated with the number of ova/embryos during superovulation at 13-18 months (r = 0.641, P < 0.05). These results suggest that the 25th and 75th percentile values of AMH concentration would give a useful rough estimate of ovarian response; however, repeated superovulation may reduce the predictive accuracy of single measurements of AMH concentration. It would be possible to evaluate AMH concentration in heifers after approximately 11 months of age.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/sangue
Bovinos/fisiologia
Transferência Embrionária/veterinária
Folículo Ovariano/fisiologia
Superovulação
[Mh] Termos MeSH secundário: Animais
Embrião de Mamíferos/fisiologia
Ciclo Estral/fisiologia
Feminino
Ovário/fisiologia
Progesterona/sangue
Curva ROC
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4G7DS2Q64Y (Progesterone); 80497-65-0 (Anti-Mullerian Hormone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1262/jrd.2016-019


  10 / 1814 MEDLINE  
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[PMID]:27845267
[Au] Autor:Zhang J; Fang L; Shi L; Lai Z; Lu Z; Xiong J; Wu M; Luo A; Wang S
[Ad] Endereço:Department of Obstetrics and Gynecology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China. Electronic address: 824754662@qq.com.
[Ti] Título:Protective effects and mechanisms investigation of Kuntai capsule on the ovarian function of a novel model with accelerated aging ovaries.
[So] Source:J Ethnopharmacol;195:173-181, 2017 Jan 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Kuntai capsule, a traditional Chinese medicine, has been widely used for the clinical treatment of menopausal syndrome. However, its mechanisms remain poorly understood. Considering that aging ovaries are the primary cause of menopause, this study was designed to investigate the effects and mechanisms of Kuntai capsule on ovarian function in a novel mice model with accelerated aging ovaries. MATERIALS AND METHODS: Seventy-five female C57BL/6 mice were chosen for this study. Fifteen of the mice were separated into the normal control group (NC). The remaining sixty were used to establish the novel accelerated aging ovary model by superovulation and oxidative stress and then by randomly dividing the mice into four equal groups. One group was considered the model group (Mod). The other three groups were treated with low (0.4g/kg), middle (0.8g/kg) and high (1.6g/kg) doses of Kuntai capsule intragastrically every day for 4 weeks. During the treatment, the body weight and fur condition of all mice were recorded. All the mice were forced to swim to record their exhaustive swimming time (EST), which measures their strength. Mice were then sacrificed for sampling. Ovarian reserve was evaluated using follicle counts and AMH expression. Ovarian function was evaluated using estrous cycle, sex hormone level and litter experiments. Ovarian follicles were categorized and counted to estimate ovarian reserve, and ovarian histologic sections were stained for terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) to detect apoptotic cells. The ultrastructure of ovarian cells was observed using transmission electron microscopy. Western blotting was used to measure expression of Bax, Bcl2, AMH and SOD2 protein. RESULTS: Compared with the NC GROUP, the Mod group clearly displayed worse fur condition and ovarian function. These situations showed some improvement after Kuntai capsule treatment. Specifically, the fur condition and the EST of the Kuntai capsule groups were superior to the fur condition and EST of the Mod group. In cases of damaged ovarian function, Kuntai capsule can regulate the estrous cycles, increase hormone secretion and fertility and significantly decrease atretic follicles. The transmission electron microscopy results revealed that Kuntai capsule rescued the ovarian ultrastructure of mice. TUNEL staining confirmed that the apoptotic cells were reduced after Kuntai capsule treatment. Western blotting revealed that Kuntai capsule can increase AMH, SOD2, and Bcl2 protein expression and decrease Bax expression. CONCLUSIONS: Kuntai capsule may improve damaged ovarian function, which may be related to its antioxidant and anti-apoptosis effects.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
Reserva Ovariana/efeitos dos fármacos
Ovário/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Oral
Fatores Etários
Animais
Hormônio Antimülleriano/metabolismo
Antioxidantes/administração & dosagem
Apoptose/efeitos dos fármacos
Cápsulas
Citoproteção
Relação Dose-Resposta a Droga
Medicamentos de Ervas Chinesas/administração & dosagem
Ciclo Estral/efeitos dos fármacos
Feminino
Fertilidade/efeitos dos fármacos
Exposição por Inalação
Camundongos Endogâmicos C57BL
Força Muscular
Tamanho do Órgão
Folículo Ovariano/efeitos dos fármacos
Folículo Ovariano/ultraestrutura
Ovário/metabolismo
Ovário/fisiopatologia
Ovário/ultraestrutura
Ozônio/toxicidade
Gravidez
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Superovulação
Superóxido Dismutase/metabolismo
Fatores de Tempo
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Bax protein, mouse); 0 (Capsules); 0 (Drugs, Chinese Herbal); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (bcl-2-Associated X Protein); 0 (kuntai capsule); 114100-40-2 (Bcl2 protein, mouse); 66H7ZZK23N (Ozone); 80497-65-0 (Anti-Mullerian Hormone); EC 1.15.1.1 (Superoxide Dismutase); EC 1.15.1.1 (superoxide dismutase 2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE



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