[PMID]: | 28684460 |
[Au] Autor: | Yuan PQ; Taché Y |
[Ad] Endereço: | CURE/Digestive Diseases Research Center, Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Digestive Diseases Division, Department of Medicine and Brain Research Institute, University of California, Los Angeles, California; and pqyuan@mednet.ucla.edu. |
[Ti] Título: | Abdominal surgery induced gastric ileus and activation of M1-like macrophages in the gastric myenteric plexus: prevention by central vagal activation in rats. |
[So] Source: | Am J Physiol Gastrointest Liver Physiol;313(4):G320-G329, 2017 Oct 01. |
[Is] ISSN: | 1522-1547 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Inflammation plays a role in abdominal surgery (AS)-induced intestinal ileus that is alleviated by electrical vagal stimulation. Intracisternal injection of RX-77368, the stable thyrotropin-releasing hormone agonist, activates dorsal motor nucleus neurons and gastric vagal efferent discharges. We investigated the gastric inflammation induced by AS and the modulation by intracisternal RX-77368 in rats. RX-77368 (50 ng/rat) or saline was injected followed, 1 h later, by laparotomy and small intestinal/cecal manipulation. The sham group had anesthesia alone. After 6 h, gastric emptying (GE) and the inflammation in gastric corpus were determined. AS inhibited GE by 72% vs. control and doubled the number of M1-like macrophage immunoreactive for major histocompatibility complex class II (MHCII; M1 marker) but not for cluster of differentiation 206 (CD206; M2 marker) (MHCII /CD206 ) while there was no change in M2-like macrophages (MHCII /CD206 ). AS increased mRNA levels of interleukin-1ß (IL-1ß) and tumor necrosis factor α (TNF-α) by 1.7- and 1.5-fold, respectively, in the gastric submucosa plus muscle layers and the infiltration of neutrophils labeled by myeloperoxidase by 9.5-fold in the muscularis externa. RX-77368 inhibited AS-related gastric changes while not altering these parameters in the sham group. There was a significant negative correlation between GE and IL-1ß ( = -0.46), TNF-α ( = -0.44), M1 macrophage ( = -0.82), and neutrophils ( = -0.91). The M2-like macrophages and IL-10 expression were unchanged by AS with intracisternal saline or RX-77368. These data indicate that AS activates gastric M1 macrophages and increases proinflammatory cytokines expression, which are prevented by central vagal activation and may contribute to the correlated dampening of postoperative gastric ileus. MHCII /CD206 (M1) and MHCII /CD206 (M2) constitute two distinct populations of macrophages that are in close apposition to the cholinergic neurons in the rat gastric myenteric plexus (MP). Abdominal surgery (6 h) activates M1 macrophage leading to inflammation in the gastric MP correlated with the delayed gastric emptying, which was abolished by central vagal stimulation via intracisternal injection of RX-77368. Vagal stimulation linked with the cephalic phase may have potential beneficial effects to curtail postoperative gastric ileus. |
[Mh] Termos MeSH primário: |
Enterostomia/efeitos adversos Motilidade Gastrointestinal/imunologia Pseudo-Obstrução Intestinal/imunologia Pseudo-Obstrução Intestinal/prevenção & controle Ativação de Macrófagos/imunologia Plexo Mientérico/fisiopatologia Nervo Vago/fisiopatologia
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[Mh] Termos MeSH secundário: |
Animais Motilidade Gastrointestinal/efeitos dos fármacos Pseudo-Obstrução Intestinal/etiologia Ativação de Macrófagos/efeitos dos fármacos Masculino Plexo Mientérico/efeitos dos fármacos Complexo Mioelétrico Migratório/efeitos dos fármacos Complexo Mioelétrico Migratório/imunologia Ácido Pirrolidonocarboxílico/administração & dosagem Ácido Pirrolidonocarboxílico/análogos & derivados Ratos Ratos Sprague-Dawley Hormônio Liberador de Tireotropina/administração & dosagem Hormônio Liberador de Tireotropina/análogos & derivados Resultado do Tratamento Nervo Vago/efeitos dos fármacos
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 5Y5F15120W (Thyrotropin-Releasing Hormone); 76820-40-1 (L-pyroglutamyl-L-histidyl-3,3-dimethylprolinamide); SZB83O1W42 (Pyrrolidonecarboxylic Acid) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171006 |
[Lr] Data última revisão:
| 171006 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170708 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1152/ajpgi.00121.2017 |
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