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[PMID]:27774905
[Au] Autor:Imbrogno A; Biscarat J; Schafer AI
[Ad] Endereço:Karlsruhe Institute of Technology, Institute of Functional Interfaces, Membrane Technology Department, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
[Ti] Título:Estradiol Uptake in a Combined Magnetic Ion Exchange - Ultrafiltration (MIEX-UF) Process During Water Treatment.
[So] Source:Curr Pharm Des;23(2):328-337, 2017.
[Is] ISSN:1873-4286
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Estrogens and their synthetic analogues are widely used as pharmaceuticals. Upon oral administration these drugs are eventually excreted via urine. The persistence of these pharmaceuticals and inefficient removal by water treatment lead to accumulation in surface water and effluents with negative effects for aquatic life and human health. METHODS: In this study, the uptake of estradiol by a combined magnetic ion exchange resin - ultrafiltration process (MIEX-UF) was investigated. This is a relatively common process used in drinking water treatment for the removal of natural organic matter. However, uptake of micropollutants, such as steroidal pharmaceuticals, may occur as a side effect of water treatment due to the high affinity for polymeric materials. To elucidate the mechanism governing estradiol partitioning between water, resin and membrane, the influence of different parameters, such as pH, humic acid concentration and membrane molecular-weight-cut-off (MWCO) was studied. RESULTS: Humic acid concentration and pH affected estradiol uptake most. At pH 11 the most significant increase of estradiol uptake was observed for MIEX-UF process (30 ng/g corresponding to 80%) compared with individual UF (17 ng/g corresponding to 12%). The presence of humic acid slightly reduced estradiol uptake at pH 11 (about 55%) due to competition for the ion exchange binding sites. CONCLUSION: Results demonstrated that the uptake of estradiol, which is amongst the most potent EDCs detected in surface water, in the MIEX-UF process can reach significant quantities (30 ng/g of resin) leading to uncontrolled accumulation of this micropollutant during drinking water treatment. This study gives a novel contribution in the understanding the mechanism of the unanticipated accumulation of pharmaceuticals, such as estradiol, in the drinking water treatment process.
[Mh] Termos MeSH primário: Estradiol/isolamento & purificação
Troca Iônica
Campos Magnéticos
Ultrafiltração/métodos
Purificação da Água/métodos
Água/química
[Mh] Termos MeSH secundário: Estradiol/química
Seres Humanos
Substâncias Húmicas
Concentração de Íons de Hidrogênio
Resinas de Troca Iônica/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Humic Substances); 0 (Ion Exchange Resins); 059QF0KO0R (Water); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.2174/1381612822666161021142412


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[PMID]:29381292
[Au] Autor:Pansini M; Dell'Agli G; Marocco A; Netti PA; Battista E; Lettera V; Vergara P; Allia P; Bonelli B; Tiberto P; Barrera G; Alberto G; Martra G; Arletti R; Esposito S
[Ti] Título:Preparation and Characterization of Magnetic and Porous Metal-Ceramic Nanocomposites from a Zeolite Precursor and Their Application for DNA Separation.
[So] Source:J Biomed Nanotechnol;13(3):337-48, 2017 Mar.
[Is] ISSN:1550-7033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this work, metal-ceramic nanocomposites were obtained through short (up to 2 h) thermal treatments at relatively moderate temperatures (750­800 °C) under a reducing atmosphere, using Fe-exchanged zeolite A as the precursor. The as-obtained materials were characterized by X-ray powder diffraction analysis, N2 adsorption at ­196 °C, and highresolution transmission electron microscopy. The results of these analyses showed that the nanocomposites consisted of a dispersion of metallic Fe nanoparticles within a porous ceramic matrix, mainly based on amorphous silica and alumina. These nanocomposites were magnetically characterized, and their magnetic response was studied. Finally, the obtained metal-ceramic nanocomposite materials were used in the separation of Escherichia coli DNA from a crude cell lysate. The results of the DNA separation experiments showed that the obtained materials could perform this type of separation.
[Mh] Termos MeSH primário: DNA Bacteriano/isolamento & purificação
DNA Bacteriano/efeitos da radiação
Separação Imunomagnética/métodos
Nanocompostos/química
Nanocompostos/ultraestrutura
Ultrafiltração/métodos
Zeolitas/química
[Mh] Termos MeSH secundário: DNA Bacteriano/química
Campos Magnéticos
Teste de Materiais
Ligas Metalo-Cerâmicas/química
Nanocompostos/efeitos da radiação
Nanoporos/ultraestrutura
Tamanho da Partícula
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Metal Ceramic Alloys); 1318-02-1 (Zeolites)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE


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[PMID]:29175695
[Au] Autor:Xie Y; Shao N; Jin Y; Zhang L; Jiang H; Xiong N; Su F; Xu H
[Ad] Endereço:Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
[Ti] Título:Determination of non-liposomal and liposomal doxorubicin in plasma by LC-MS/MS coupled with an effective solid phase extraction: In comparison with ultrafiltration technique and application to a pharmacokinetic study.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:149-160, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Liposomal formulation of doxorubicin has been widely applied in clinic for treatment of various cancers. The separation and measurement of free drug (drug which is not entrapped in liposomes) and liposomal drug in the plasma after injection of liposomal doxorubicin is of prime importance due to toxicity and activity concerns. In this study, a rapid and convenient method was developed to isolate and determine the non-liposomal and liposomal drugs in plasma. Plasma samples were prepared by solid phase extraction (SPE) using Oasis HLB cartridges. Liposomal doxorubicin (L-DOX) was collected in the aqueous eluate with its internal standard (IS), metformin; and non-liposomal doxorubicin (NL-DOX) and its isotope labelling IS were eluted from the cartridge by methanol containing 0.5% formic acid. After SPE separation, L-DOX and NL-DOX were subsequently quantified by a validated sensitive LC-MS/MS method individually. The calibration curves were found to be linear for L-DOX in the range of 0.156-40.0µg/mL and for NL-DOX in the range of 3.13-200ng/mL. The extraction recovery was about 97% for L-DOX and about 65% for NL-DOX. This method was further applied to investigate the pharmacokinetics of doxorubicin in Beagle dogs after an intravenous dose of 1.0mg/kg Doxil . After injection of Doxil , L-DOX was the predominant component circulating in plasma, whose amount was about 1000-fold higher than that of NL-DOX. The analytical method might be helpful in pharmacokinetics and toxicity assessment of liposomal formulation.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Doxorrubicina/análogos & derivados
Extração em Fase Sólida/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Cães
Doxorrubicina/sangue
Doxorrubicina/química
Doxorrubicina/farmacocinética
Feminino
Seres Humanos
Limite de Detecção
Modelos Lineares
Masculino
Polietilenoglicóis/química
Polietilenoglicóis/farmacocinética
Reprodutibilidade dos Testes
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (liposomal doxorubicin); 30IQX730WE (Polyethylene Glycols); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29251901
[Au] Autor:Zhang L; Zhang P; Wang M; Yang K; Liu J
[Ti] Título:Research on the experiment of reservoir water treatment applying ultrafiltration membrane technology of different processes.
[So] Source:J Environ Biol;37(5):1007-12, 2016 09.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The processes and effects of coagulation-ultrafiltration (C-UF) and coagulation sedimentation-ultrafiltration (CS-UF) process used in the treatment of Dalangdian Reservoir water were compared. The experiment data indicated that 99% of turbidity removal and basically 100% of microorganism and algae removal were achieved in both C-UF and CS-UF process. The organic removal effect of CS-UF? process was slightly better than C-UF process. However, the organic removal effect under different processes was not obvious due to limitation of ultrafiltration membrane aperture. Polyaluminium chloride was taken as a coagulant in water plant. The aluminum ion removal result revealed that coagulant dosage was effectively saved by using membrane technology during megathermal high algae laden period. Within the range of certain reagent concentration and soaking time, air-water backwashing of every filtration cycle of membrane was conducted to effectively reduce membrane pollution. Besides, maintenance cleaning was conducted every 60 min. whether or not restorative cleaning was conducted depends on the pollution extent. After cleaning, recovery of membrane filtration effect was obvious.
[Mh] Termos MeSH primário: Ultrafiltração/instrumentação
Ultrafiltração/métodos
Purificação da Água
Abastecimento de Água
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Membranas Artificiais
[Pt] Tipo de publicação:RESEARCH SUPPORT, NON-U.S. GOV'T; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29406122
[Au] Autor:Wang X; Ma B; Bai Y; Lan H; Liu H; Qu J
[Ad] Endereço:Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Comparison of the effects of aluminum and iron(III) salts on ultrafiltration membrane biofouling in drinking water treatment.
[So] Source:J Environ Sci (China);63:96-104, 2018 Jan.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Coagulation plays an important role in alleviating membrane fouling, and a noticeable problem is the development of microorganisms after long-time operation, which gradually secrete extracellular polymeric substances (EPS). To date, few studies have paid attention to the behavior of microorganisms in drinking water treatment with ultrafiltration (UF) membranes. Herein, the membrane biofouling was investigated with different aluminum and iron salts. We found that Al (SO ) ·18H O performed better in reducing membrane fouling due to the slower growth rate of microorganisms. In comparison to Al (SO ) ·18H O, more EPS were induced with Fe (SO ) ·xH O, both in the membrane tank and the sludge on the cake layer. We also found that bacteria were the major microorganisms, of which the concentration was much higher than those of fungi and archaea. Further analyses showed that Proteobacteria was dominant in bacterial communities, which caused severe membrane fouling by forming a biofilm, especially for Fe (SO ) ·xH O. Additionally, the abundances of Bacteroidetes and Verrucomicrobia were relatively higher in the presence of Al (SO ) ·18H O, resulting in less severe biofouling by effectively degrading the protein and polysaccharide in EPS. As a result, in terms of microorganism behaviors, Al-based salts should be given preference as coagulants during actual operations.
[Mh] Termos MeSH primário: Incrustação Biológica
Ferro/química
Sais/química
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Alumínio
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Salts); CPD4NFA903 (Aluminum); E1UOL152H7 (Iron)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


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[PMID]:28464237
[Au] Autor:Baek Y; Singh N; Arunkumar A; Borys M; Li ZJ; Zydney AL
[Ad] Endereço:Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802.
[Ti] Título:Ultrafiltration behavior of monoclonal antibodies and Fc-fusion proteins: Effects of physical properties.
[So] Source:Biotechnol Bioeng;114(9):2057-2065, 2017 09.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ultrafiltration (UF) is used for the final concentration and formulation of essentially all antibody-based therapeutics including both monoclonal antibodies (mAbs) and Fc-fusion proteins. The objective of this study was to quantitatively compare the filtrate flux behavior for two highly purified mAbs and an Fc-fusion protein under identical flow and buffer conditions. Filtrate flux data were obtained using a Pellicon 3 tangential flow filtration cassette over a wide range of transmembrane pressures and bulk protein concentrations. Independent experimental measurements were performed to evaluate the protein osmotic pressure and solution viscosity. The maximum achievable protein concentration was directly correlated with the solution viscosity, which controls the pressure drop and extent of back-filtration in the cassette. The filtrate flux data were analyzed using a recently developed model that accounts for the effects of intermolecular interactions and transmembrane pressure gradients on the extent of concentration polarization. These results provide important insights into the factors controlling the filtrate flux during the UF of concentrated protein solutions and an effective framework for the design/analysis of UF processes for the formulation of antibody-based therapeutics. Biotechnol. Bioeng. 2017;114: 2057-2065. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/química
Anticorpos Monoclonais/isolamento & purificação
Composição de Medicamentos/métodos
Fragmentos Fc das Imunoglobulinas/química
Fragmentos Fc das Imunoglobulinas/isolamento & purificação
Ultrafiltração/métodos
[Mh] Termos MeSH secundário: Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Immunoglobulin Fc Fragments); 0 (Recombinant Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26326


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[PMID]:28464235
[Au] Autor:Yang Y; Velayudhan A; Thornhill NF; Farid SS
[Ad] Endereço:Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
[Ti] Título:Multi-criteria manufacturability indices for ranking high-concentration monoclonal antibody formulations.
[So] Source:Biotechnol Bioeng;114(9):2043-2056, 2017 09.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The need for high-concentration formulations for subcutaneous delivery of therapeutic monoclonal antibodies (mAbs) can present manufacturability challenges for the final ultrafiltration/diafiltration (UF/DF) step. Viscosity levels and the propensity to aggregate are key considerations for high-concentration formulations. This work presents novel frameworks for deriving a set of manufacturability indices related to viscosity and thermostability to rank high-concentration mAb formulation conditions in terms of their ease of manufacture. This is illustrated by analyzing published high-throughput biophysical screening data that explores the influence of different formulation conditions (pH, ions, and excipients) on the solution viscosity and product thermostability. A decision tree classification method, CART (Classification and Regression Tree) is used to identify the critical formulation conditions that influence the viscosity and thermostability. In this work, three different multi-criteria data analysis frameworks were investigated to derive manufacturability indices from analysis of the stress maps and the process conditions experienced in the final UF/DF step. Polynomial regression techniques were used to transform the experimental data into a set of stress maps that show viscosity and thermostability as functions of the formulation conditions. A mathematical filtrate flux model was used to capture the time profiles of protein concentration and flux decay behavior during UF/DF. Multi-criteria decision-making analysis was used to identify the optimal formulation conditions that minimize the potential for both viscosity and aggregation issues during UF/DF. Biotechnol. Bioeng. 2017;114: 2043-2056. © 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Perodicals, Inc.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/química
Anticorpos Monoclonais/isolamento & purificação
Composição de Medicamentos/métodos
Composição de Medicamentos/normas
Ultrafiltração/métodos
Ultrafiltração/normas
[Mh] Termos MeSH secundário: Estabilidade de Medicamentos
Guias como Assunto
Temperatura Ambiente
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26329


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[PMID]:27776242
[Au] Autor:Khansary MA; Mellat M; Saadat SH; Fasihi-Ramandi M; Kamali M; Taheri RA
[Ad] Endereço:Young Researchers and Elite Club, South Tehran Branch, Islamic Azad University, Tehran, Iran. Electronic address: miladasgarpour@ut.ac.ir.
[Ti] Título:An enquiry on appropriate selection of polymers for preparation of polymeric nanosorbents and nanofiltration/ultrafiltration membranes for hormone micropollutants removal from water effluents.
[So] Source:Chemosphere;168:91-99, 2017 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To analyze polymeric nanosorbents and nanofiltration/ultrafiltration membranes for hormone micropollutants removal from water effluents, here an in-through investigation on the suitability and compatibility of various polymers has been carried out. For this work, estradiol, estrone, testosterone, progesterone, estriol, mestranol, and ethinylestradiol were considered. A total number of 452 polymers were analyzed and initially screened using Hansen solubility parameters. The identified good pairs of hormones and polymers then were examined to obtain the equilibrium capacity of hormones removal from water effluents using a modified Flory-Huggins model. A distribution coefficient was defined as the ratio of hormones in water effluent phase and polymer phase. For removal of mestranol, estradiol and ethinylestradiol, no compatible polymer was identified based on initial screening of collected database. Three compatible polymers were identified for estriol. For progesterone, a wide variety of polymers was identified as good matching of polar, dispersion and hydrogen forces contributions can be observed for these pairs. For estrone, only two polymers can be proposed due to the mismatch observed between polar, dispersion and hydrogen forces contributions of other polymers and this hormone. The phase calculations showed that not all the identified good pairs could be used for practical separation applications. The domain of applicability of each good pair was investigated and potential polymers for practical micropollutants removal together with their removal capacity were represented in terms of phase envelops. The theoretical approach follows fundamental chemical thermodynamic equations and then can be simply applied for any system of interest.
[Mh] Termos MeSH primário: Hormônios/análise
Membranas Artificiais
Nanoestruturas/química
Polímeros/química
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Adsorção
Modelos Teóricos
Solubilidade
Termodinâmica
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hormones); 0 (Membranes, Artificial); 0 (Polymers); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28873590
[Au] Autor:Abdelhedi O; Nasri R; Mora L; Jridi M; Toldrá F; Nasri M
[Ad] Endereço:Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia. Electronic address: abd.ola1502@gmail.com.
[Ti] Título:In silico analysis and molecular docking study of angiotensin I-converting enzyme inhibitory peptides from smooth-hound viscera protein hydrolysates fractionated by ultrafiltration.
[So] Source:Food Chem;239:453-463, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Smooth-hound viscera hydrolysates (SHVHs) were prepared by treatment with Neutrase (SHVH-N) and Purafect (SHVH-P). Hydrolysates were then separated according to their molecular weight, using the ultra-filtration membrane system, into 5 fractions (≥50, 50-5, 5-3, 3-1 and ≤1kDa). Fractions showed different amino acid compositions and angiotensin I-converting enzyme (ACE) inhibitory potentials. The SHVH-P-FV (≤1kDa) and SHVH-N-FIV (3-1kDa) fractions showed the best ACE-inhibitory activities with IC values of 53.31 and 75.05µg/ml, respectively. According to their high ACE-inhibitory potential, FIV and FV were fractionated by RP-HPLC and then analyzed by LC-MS/MS to identify peptide sequences. A systematic peptidomic study resulted in the identification of numerous novel sequences. Furthermore, in silico data, based on the molecular docking simulation, showed that GPAGPRGPAG, AVVPPSDKM, TTMYPGIA, and VKPLPQSG could bind ACE active site with low interaction scores. Indeed, they share hydrogen bonds and Van der Waals and electrostatic interactions with ACE catalytic pockets.
[Mh] Termos MeSH primário: Vísceras
[Mh] Termos MeSH secundário: Inibidores da Enzima Conversora de Angiotensina
Simulação de Acoplamento Molecular
Peptídeos
Peptidil Dipeptidase A
Hidrolisados de Proteína
Espectrometria de Massas em Tandem
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Peptides); 0 (Protein Hydrolysates); EC 3.4.15.1 (Peptidyl-Dipeptidase A)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  10 / 9377 MEDLINE  
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[PMID]:29023592
[Au] Autor:Niu Z; Pang RTK; Liu W; Li Q; Cheng R; Yeung WSB
[Ad] Endereço:Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
[Ti] Título:Polymer-based precipitation preserves biological activities of extracellular vesicles from an endometrial cell line.
[So] Source:PLoS One;12(10):e0186534, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extracellular vesicles (EVs) are membrane-bound vesicles released by cells and act as media for transfer of proteins, small RNAs and mRNAs to distant sites. They can be isolated by different methods. However, the biological activities of the purified EVs have seldom been studied. In this study, we compared the use of ultracentrifugation (UC), ultra-filtration (UF), polymer-based precipitation (PBP), and PBP with size-based purification (PBP+SP) for isolation of EVs from human endometrial cells and mouse uterine luminal fluid (ULF). Electron microscopy revealed that the diameters of the isolated EVs were similar among the tested methods. UF recovered the highest number of EVs followed by PBP, while UC and PBP+SP were significantly less efficient (P<0.05). Based on the number of EVs-to-protein ratios, PBP had the least protein contamination, significantly better than the other methods (P<0.05). All the isolated EVs expressed exosome-enriched proteins CD63, TSG101 and HSP70. Incubation of the trophoblast JEG-3 cells with an equal amount of the fluorescence-labelled EVs isolated by the studied methods showed that many of the PBP-EVs treated cells were fluorescence positive but only a few cells were labelled in the UC- and UF-EVs treated groups. Moreover, the PBP-EVs could transfer significantly more miRNA to the recipient cells than the other 3 methods (P<0.05). The PBP method could isolate EVs from mouse ULF; the diameter of the isolated EVs was 62±19 nm and expressed CD63, TSG101 and HSP70 proteins. In conclusion, PBP could best preserve the activities of the isolated EVs among the 4 methods studied and was able to isolate EVs from a small volume of sample. The simple setup and low equipment demands makes PBP the most suitable method for rapid EV assessment and isolation of EVs in clinical and basic research settings.
[Mh] Termos MeSH primário: Vesículas Extracelulares/metabolismo
Polímeros/química
[Mh] Termos MeSH secundário: Compostos de Anilina/farmacologia
Animais
Compostos de Benzilideno/farmacologia
Blastocisto/citologia
Blastocisto/metabolismo
Linhagem Celular
Precipitação Química
Técnicas de Cocultura
Proteínas de Ligação a DNA/metabolismo
Neoplasias do Endométrio/metabolismo
Neoplasias do Endométrio/patologia
Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo
Exossomos/efeitos dos fármacos
Exossomos/metabolismo
Vesículas Extracelulares/ultraestrutura
Feminino
Proteínas de Choque Térmico HSP70/metabolismo
Seres Humanos
Camundongos
MicroRNAs/metabolismo
Microscopia Eletrônica
Microscopia de Fluorescência
Tetraspanina 30/metabolismo
Fatores de Transcrição/metabolismo
Trofoblastos/citologia
Trofoblastos/metabolismo
Ultracentrifugação
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Benzylidene Compounds); 0 (DNA-Binding Proteins); 0 (Endosomal Sorting Complexes Required for Transport); 0 (GW 4869); 0 (HSP70 Heat-Shock Proteins); 0 (MicroRNAs); 0 (Polymers); 0 (Tetraspanin 30); 0 (Transcription Factors); 0 (Tsg101 protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186534



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