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  1 / 13168 MEDLINE  
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[PMID]:29234803
[Au] Autor:Maas E; Juch J; Huygen F
[Ad] Endereço:Department of Health Sciences, VU University Amsterdam, Amsterdam, the Netherlands.
[Ti] Título:Radiofrequency Denervation for Chronic Low Back Pain-Reply.
[So] Source:JAMA;318(22):2256-2257, 2017 12 12.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Denervação
Dor Lombar/cirurgia
[Mh] Termos MeSH secundário: Seres Humanos
Vértebras Lombares
Ondas de Rádio
Resultado do Tratamento
Articulação Zigapofisária
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.16390


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[PMID]:29234800
[Au] Autor:Vorobeychik Y; Stojanovic MP; McCormick ZL
[Ad] Endereço:Department of Anesthesiology, Penn State Milton S. Hershey Medical Center, Hershey, Pennsylvania.
[Ti] Título:Radiofrequency Denervation for Chronic Low Back Pain.
[So] Source:JAMA;318(22):2254-2255, 2017 12 12.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Denervação
Dor Lombar/cirurgia
[Mh] Termos MeSH secundário: Dor nas Costas/cirurgia
Seres Humanos
Vértebras Lombares
Ondas de Rádio
Resultado do Tratamento
Articulação Zigapofisária
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.16386


  3 / 13168 MEDLINE  
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[PMID]:29234799
[Au] Autor:Kao MC; Leong MS; Mackey S
[Ad] Endereço:Department of Anesthesiology, Stanford University, Stanford, California.
[Ti] Título:Radiofrequency Denervation for Chronic Low Back Pain.
[So] Source:JAMA;318(22):2256, 2017 12 12.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Denervação
Dor Lombar/cirurgia
[Mh] Termos MeSH secundário: Dor nas Costas/cirurgia
Seres Humanos
Vértebras Lombares
Ondas de Rádio
Resultado do Tratamento
Articulação Zigapofisária
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.16382


  4 / 13168 MEDLINE  
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[PMID]:29234798
[Au] Autor:Rimmalapudi V; Buchalter J; Calodney A
[Ad] Endereço:Gulf Coast Pain Institute, Pensacola, Florida.
[Ti] Título:Radiofrequency Denervation for Chronic Low Back Pain.
[So] Source:JAMA;318(22):2255-2256, 2017 12 12.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Denervação
Dor Lombar/cirurgia
[Mh] Termos MeSH secundário: Dor nas Costas/cirurgia
Seres Humanos
Vértebras Lombares
Ondas de Rádio
Resultado do Tratamento
Articulação Zigapofisária
[Pt] Tipo de publicação:LETTER; COMMENT
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.16378


  5 / 13168 MEDLINE  
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[PMID]:28854256
[Au] Autor:Debaud C; Salga M; Begot L; Holy X; Chedik M; de l'Escalopier N; Torossian F; Levesque JP; Lataillade JJ; Le Bousse-Kerdilès MC; Genêt F
[Ad] Endereço:Spine Division Orthopaedic Surgery Department, Hôpital Européen Georges Pompidou, APHP, Paris, France.
[Ti] Título:Peripheral denervation participates in heterotopic ossification in a spinal cord injury model.
[So] Source:PLoS One;12(8):e0182454, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We previously reported the development of a new acquired neurogenic HO (NHO) mouse model, combining spinal cord transection (SCI) and chemical muscle injury. Pathological mechanisms responsible for ectopic osteogenesis after central neurological damage are still to be elucidated. In this study, we first hypothesized that peripheral nervous system (PNS) might convey pathological signals from injured spinal cord to muscles in NHO mouse model. Secondly, we sought to determine whether SCI could lead to intramuscular modifications of BMP2 signaling pathways. Twenty one C57Bl6 mice were included in this protocol. Bilateral cardiotoxin (CTX) injection in hamstring muscles was associated with a two-stage surgical procedure, combining thoracic SCI with unilateral peripheral denervation. Volumes of HO (Bone Volume, BV) were measured 28 days after surgery using micro-computed tomography imaging techniques and histological analyses were made to confirm intramuscular osteogenesis. Volume comparisons were conducted between right and left hind limb of each animal, using a Wilcoxon signed rank test. Quantitative polymerase chain reaction (qPCR) was performed to explore intra muscular expression of BMP2, Alk3 and Id1. Nineteen mice survive the complete SCI and peripheral denervation procedure. When CTX injections were done right after surgery (n = 7), bilateral HO were detected in all animals after 28 days. Micro-CT measurements showed significantly increased BV in denervated paws (1.47 mm3 +/- 0.5) compared to contralateral sides (0.56 mm3 +/-0.4), p = 0.03. When peripheral denervation and CTX injections were performed after sham SCI surgery (n = 6), bilateral HO were present in three mice at day 28. Quantitative PCR analyses showed no changes in intra muscular BMP2 expression after SCI as compared to control mice (shamSCI). Peripheral denervation can be reliably added to spinal cord transection in NHO mouse model. This new experimental design confirms that neuro inflammatory mechanisms induced by central or peripheral nervous system injury plays a key role in triggering ectopic osteogenesis.
[Mh] Termos MeSH primário: Músculos/patologia
Ossificação Heterotópica/patologia
Traumatismos da Medula Espinal/patologia
Medula Espinal/patologia
[Mh] Termos MeSH secundário: Animais
Proteína Morfogenética Óssea 2/análise
Proteínas Cardiotóxicas de Elapídeos
Denervação
Modelos Animais de Doenças
Feminino
Camundongos Endogâmicos C57BL
Músculos/efeitos dos fármacos
Músculos/inervação
Ossificação Heterotópica/induzido quimicamente
Ossificação Heterotópica/diagnóstico por imagem
Ossificação Heterotópica/etiologia
Medula Espinal/diagnóstico por imagem
Medula Espinal/efeitos dos fármacos
Traumatismos da Medula Espinal/induzido quimicamente
Traumatismos da Medula Espinal/diagnóstico por imagem
Traumatismos da Medula Espinal/etiologia
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Cobra Cardiotoxin Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182454


  6 / 13168 MEDLINE  
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[PMID]:28842421
[Au] Autor:Hernández RG; Benítez-Temiño B; Morado-Díaz CJ; Davis-López de Carrizosa MA; de la Cruz RR; Pastor AM
[Ad] Endereço:Departamento Fisiología, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain.
[Ti] Título:Effects of Selective Deafferentation on the Discharge Characteristics of Medial Rectus Motoneurons.
[So] Source:J Neurosci;37(38):9172-9188, 2017 Sep 20.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Medial rectus motoneurons receive two main pontine inputs: abducens internuclear neurons, whose axons course through the medial longitudinal fasciculus (MLF), and neurons in the lateral vestibular nucleus, whose axons project through the ascending tract of Deiters (ATD). Abducens internuclear neurons are responsible for conjugate gaze in the horizontal plane, whereas ATD neurons provide medial rectus motoneurons with a vestibular input comprising mainly head velocity. To reveal the relative contribution of each input to the oculomotor physiology, single-unit recordings from medial rectus motoneurons were obtained in the control situation and after selective deafferentation from cats with unilateral transection of either the MLF or the ATD. Both MLF and ATD transection produced similar short-term alterations in medial rectus motoneuron firing pattern, which were more drastic in MLF of animals. However, long-term recordings revealed important differences between the two types of lesion. Thus, while the effects of the MLF section were permanent, 2 months after ATD lesioning all motoneuronal firing parameters were similar to the control. These findings indicated a more relevant role of the MLF pathway in driving motoneuronal firing and evidenced compensatory mechanisms following the ATD lesion. Confocal immunocytochemistry revealed that MLF transection produced also a higher loss of synaptic boutons, mainly at the dendritic level. Moreover, 2 months after ATD transection, we observed an increase in synaptic coverage around motoneuron cell bodies compared with short-term data, which is indicative of a synaptogenic compensatory mechanism of the abducens internuclear pathway that could lead to the observed firing and morphological recovery. Eye movements rely on multiple neuronal circuits for appropriate performance. The abducens internuclear pathway through the medial longitudinal fascicle (MLF) and the vestibular neurons through the ascending tract of Deiters (ATD) are a dual system that supports the firing of medial rectus motoneurons. We report the effect of sectioning the MLF or the ATD pathway on the firing of medial rectus motoneurons, as well as the plastic mechanisms by which one input compensates for the lack of the other. This work shows that while the effects of MLF transection are permanent, the ATD section produces transitory effects. A mechanism based on axonal sprouting and occupancy of the vacant synaptic space due to deafferentation is the base for the mechanism of compensation on the medial rectus motoneuron.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Vias Aferentes/fisiologia
Neurônios Motores/fisiologia
Músculos Oculomotores/inervação
Músculos Oculomotores/fisiologia
Núcleos Vestibulares/fisiologia
[Mh] Termos MeSH secundário: Animais
Gatos
Denervação/métodos
Feminino
Neurônios Motores/citologia
Músculos Oculomotores/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.1391-17.2017


  7 / 13168 MEDLINE  
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[PMID]:28818859
[Au] Autor:Uzuka H; Matsumoto Y; Nishimiya K; Ohyama K; Suzuki H; Amamizu H; Morosawa S; Hirano M; Shindo T; Kikuchi Y; Hao K; Shiroto T; Ito K; Takahashi J; Fukuda K; Miyata S; Funaki Y; Ishibashi-Ueda H; Yasuda S; Shimokawa H
[Ad] Endereço:From the Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan (H.U., Y.M., K.N., K.O., H. Suzuki, H.A., S.M., M.H., T.S., Y.K., K.H., T.S., K.I., J.T., K.F., S.M., H. Shimokawa); Wellman Center for Photomedicine, Massachusetts General Hospital, Boston (
[Ti] Título:Renal Denervation Suppresses Coronary Hyperconstricting Responses After Drug-Eluting Stent Implantation in Pigs In Vivo Through the Kidney-Brain-Heart Axis.
[So] Source:Arterioscler Thromb Vasc Biol;37(10):1869-1880, 2017 Oct.
[Is] ISSN:1524-4636
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Drug-eluting stent-induced coronary hyperconstricting responses remain an important issue. The adventitia harbors a variety of components that potently modulate vascular tone, including sympathetic nerve fibers (SNF) and vasa vasorum. Catheter-based renal denervation (RDN) inhibits sympathetic nerve activity. We, thus, examined whether RDN suppresses drug-eluting stent-induced coronary hyperconstricting responses, and if so, what mechanisms are involved. APPROACH AND RESULTS: Protocol 1: pigs implanted with everolimus-eluting stents into the left coronary arteries underwent coronary angiography at 1 month after implantation for assessment of coronary vasomotion and adventitial SNF formation. Drug-eluting stent-induced coronary hyperconstricting responses were significantly enhanced associated with enhanced coronary adventitial SNF and vasa vasorum formation. Protocol 2: pigs implanted with everolimus-eluting stents were randomly assigned to the RDN or sham group. The RDN group underwent renal ablation. At 1 month, RDN significantly caused marked damage of the SNF at the renal arteries without any stenosis, thrombus, or dissections. Notably, RDN significantly upregulated the expression of α -adrenergic receptor-binding sites in the nucleus tractus solitarius, attenuated muscle sympathetic nerve activity, and decreased systolic blood pressure and plasma renin activity. In addition, RDN attenuated coronary hyperconstricting responses to intracoronary serotonin at the proximal and distal stent edges associated with decreases in SNF and vasa vasorum formation, inflammatory cell infiltration, and Rho-kinase expression/activation. Furthermore, there were significant positive correlations between SNF and vasa vasorum and between SNF and coronary vasoconstricting responses. CONCLUSIONS: These results provide the first evidence that RDN ameliorates drug-eluting stent-induced coronary hyperconstricting responses in pigs in vivo through the kidney-brain-heart axis.
[Mh] Termos MeSH primário: Encéfalo/fisiologia
Vasos Coronários/fisiologia
Stents Farmacológicos
Coração/inervação
Rim/inervação
Sistema Nervoso Simpático/fisiologia
Vasoconstrição
[Mh] Termos MeSH secundário: Animais
Pressão Sanguínea/fisiologia
Denervação
Stents Farmacológicos/efeitos adversos
Frequência Cardíaca/fisiologia
Suínos
Quinases Associadas a rho/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.11.1 (rho-Associated Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1161/ATVBAHA.117.309777


  8 / 13168 MEDLINE  
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[PMID]:28701323
[Au] Autor:Becker BK; Feagans AC; Chen D; Kasztan M; Jin C; Speed JS; Pollock JS; Pollock DM
[Ad] Endereço:Cardio-Renal Physiology and Medicine, Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama.
[Ti] Título:Renal denervation attenuates hypertension but not salt sensitivity in ET receptor-deficient rats.
[So] Source:Am J Physiol Regul Integr Comp Physiol;313(4):R425-R437, 2017 Oct 01.
[Is] ISSN:1522-1490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypertension is a prevalent pathology that increases risk for numerous cardiovascular diseases. Because the etiology of hypertension varies across patients, specific and effective therapeutic approaches are needed. The role of renal sympathetic nerves is established in numerous forms of hypertension, but their contribution to salt sensitivity and interaction with factors such as endothelin-1 are poorly understood. Rats deficient of functional ET receptors (ET -def) on all tissues except sympathetic nerves are hypertensive and exhibit salt-sensitive increases in blood pressure. We hypothesized that renal sympathetic nerves contribute to hypertension and salt sensitivity in ET -def rats. The hypothesis was tested through bilateral renal sympathetic nerve denervation and measuring blood pressure during normal salt (0.49% NaCl) and high-salt (4.0% NaCl) diets. Denervation reduced mean arterial pressure in ET -def rats compared with sham-operated controls by 12 ± 3 (SE) mmHg; however, denervation did not affect the increase in blood pressure after 2 wk of high-salt diet (+19 ± 3 vs. +16 ± 3 mmHg relative to normal salt diet; denervated vs. sham, respectively). Denervation reduced cardiac sympathetic-to-parasympathetic tone [low frequency-high frequency (LF/HF)] during normal salt diet and vasomotor LF/HF tone during high-salt diet in ET -def rats. We conclude that the renal sympathetic nerves contribute to the hypertension but not to salt sensitivity of ET -def rats.
[Mh] Termos MeSH primário: Pressão Sanguínea/fisiologia
Denervação
Hipertensão/genética
Hipertensão/cirurgia
Rim/inervação
Receptor de Endotelina B/genética
Cloreto de Sódio na Dieta/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Barorreflexo/efeitos dos fármacos
Barorreflexo/fisiologia
Pressão Sanguínea/efeitos dos fármacos
Hipertensão/fisiopatologia
Rim/fisiopatologia
Rim/cirurgia
Sistema Nervoso Parassimpático/fisiopatologia
Ratos
Ratos Transgênicos
Sistema Nervoso Simpático/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptor, Endothelin B); 0 (Sodium Chloride, Dietary)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1152/ajpregu.00174.2017


  9 / 13168 MEDLINE  
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[PMID]:28672319
[Au] Autor:Juch JNS; Maas ET; Ostelo RWJG; Groeneweg JG; Kallewaard JW; Koes BW; Verhagen AP; van Dongen JM; Huygen FJPM; van Tulder MW
[Ad] Endereço:Department of Anesthesiology, Erasmus University Medical Centre, Rotterdam, the Netherlands.
[Ti] Título:Effect of Radiofrequency Denervation on Pain Intensity Among Patients With Chronic Low Back Pain: The Mint Randomized Clinical Trials.
[So] Source:JAMA;318(1):68-81, 2017 Jul 04.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Importance: Radiofrequency denervation is a commonly used treatment for chronic low back pain, but high-quality evidence for its effectiveness is lacking. Objective: To evaluate the effectiveness of radiofrequency denervation added to a standardized exercise program for patients with chronic low back pain. Design, Setting, and Participants: Three pragmatic multicenter, nonblinded randomized clinical trials on the effectiveness of minimal interventional treatments for participants with chronic low back pain (Mint study) were conducted in 16 multidisciplinary pain clinics in the Netherlands. Eligible participants were included between January 1, 2013, and October 24, 2014, and had chronic low back pain, a positive diagnostic block at the facet joints (facet joint trial, 251 participants), sacroiliac joints (sacroiliac joint trial, 228 participants), or a combination of facet joints, sacroiliac joints, or intervertebral disks (combination trial, 202 participants) and were unresponsive to conservative care. Interventions: All participants received a 3-month standardized exercise program and psychological support if needed. Participants in the intervention group received radiofrequency denervation as well. This is usually a 1-time procedure, but the maximum number of treatments in the trial was 3. Main Outcomes and Measures: The primary outcome was pain intensity (numeric rating scale, 0-10; whereby 0 indicated no pain and 10 indicated worst pain imaginable) measured 3 months after the intervention. The prespecified minimal clinically important difference was defined as 2 points or more. Final follow-up was at 12 months, ending October 2015. Results: Among 681 participants who were randomized (mean age, 52.2 years; 421 women [61.8%], mean baseline pain intensity, 7.1), 599 (88%) completed the 3-month follow-up, and 521 (77%) completed the 12-month follow-up. The mean difference in pain intensity between the radiofrequency denervation and control groups at 3 months was -0.18 (95% CI, -0.76 to 0.40) in the facet joint trial; -0.71 (95% CI, -1.35 to -0.06) in the sacroiliac joint trial; and -0.99 (95% CI, -1.73 to -0.25) in the combination trial. Conclusions and Relevance: In 3 randomized clinical trials of participants with chronic low back pain originating in the facet joints, sacroiliac joints, or a combination of facet joints, sacroiliac joints, or intervertebral disks, radiofrequency denervation combined with a standardized exercise program resulted in either no improvement or no clinically important improvement in chronic low back pain compared with a standardized exercise program alone. The findings do not support the use of radiofrequency denervation to treat chronic low back pain from these sources. Trial Registration: trialregister.nl Identifier: NTR3531.
[Mh] Termos MeSH primário: Denervação/métodos
Terapia por Exercício
Dor Lombar/terapia
Ondas de Rádio/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Idoso
Dor Crônica/terapia
Terapia Combinada
Feminino
Seres Humanos
Dor Lombar/diagnóstico
Vértebras Lombares
Meia-Idade
Diferença Mínima Clinicamente Importante
Bloqueio Nervoso
Articulação Sacroilíaca
Falha de Tratamento
Articulação Zigapofisária
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; PRAGMATIC CLINICAL TRIAL; RANDOMIZED CONTROLLED TRIAL
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.7918


  10 / 13168 MEDLINE  
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[PMID]:28534336
[Au] Autor:Shi H; Tian Y; Dai F; Xiao L; Ke Z; Tong W
[Ti] Título:[Establishment of pelvic nerve denervation modal in mice].
[So] Source:Zhonghua Wei Chang Wai Ke Za Zhi;20(5):560-565, 2017 May 25.
[Is] ISSN:1671-0274
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To establishment and verify pelvic nerve denervation (PND) model in mice. METHODS: (1) Establishment of models. Seventy-two healthy male SPE class C57 mice with age of 7 weeks and body weight of (25±1) g were chosen. These 72 mice were randomly divided into PND group containing 36 mice and sham operation group containing 36 mice. Referring to the establishment method of PND rats, after anesthesia, a laparotomy was performed on the mouse with an abdominal median incision. Under the dissection microscope, the pelvic nerves behind and after each sides of the prostate gland were bluntly separated with cotton swabs and cut with a dissecting scissor. After the operation, the urination of mice was assisted twice every day. For the mice of sham operation group, the pelvic nerves were only exposed without cutting. (2) Detection of models. Colonic transit test was performed in 18 mice chosen randomly from each group to detect the colonic transit ratio (colored colon by methylene blue/ whole colon) and visceral sensitivity tests was performed in the rest mice to observe and record the changes of electromyogram. RESULTS: Three mice died of colonic transit test in each group. Uroschesis occurred in all the mice of PND group and needed bladder massage to assist the urination. Colonic transit test showed that the colonic transit ratios of sham operation group at postoperative day (POD) 1, 3 and 7 were (0.4950±0.3858)%, (0.6386±0.1293)% and (0.6470±0.1088)% without significant difference (F=0.3647, P=0.058), while in PND group, the colonic transit ratio at POD 7 [(0.6044±0.1768) %] was obviously higher than that both at POD 3[(0.3876±0.1364)%, P=0.022] and POD 1[(0.2542±0.0371)%, P=0.001], indicating a recovery trend of colonic transit function (F=9.143, P=0.004). Compared with the sham operation group, the colonic transit function in PND group decreased significantly at POD 1 and POD 3(both P<0.05), and at POD 7, there was no significant difference between two groups. Visceral sensitivity test showed that the visceral sensitivity of sham operation group at POD 1, 3 and 7 was 24.2808±9.5566, 33.6725±7.9548 and 43.9086±12.1875 with significant difference (F=5.722, P=0.014). The visceral sensitivity of PND group at POD 1, 3 and 7 was 11.7609±2.1049, 21.8415±8.1527 and 26.2310±4.2235 with significant difference as well (F=11.154, P=0.001). The visceral sensitivity at POD 3 and POD 7 was obviously higher than that at POD 1 (P=0.006, P<0.001), and there was no significant difference between POD 3 and POD 7 (P=0.183). Compared with sham operation group, the visceral sensitivity of PND group decreased significantly at POD 1, 3 and 7(all P<0.05). CONCLUSIONS: Denervation of pelvic nerves can obviously decrease the colonic transit function and the visceral sensitivity of mice, but these changes can recover over time, which suggests that the establishment of PND model in mice is successful.
[Mh] Termos MeSH primário: Vias Autônomas/cirurgia
Colo/inervação
Denervação/métodos
Modelos Animais de Doenças
Tecido Nervoso/cirurgia
Pelve/inervação
[Mh] Termos MeSH secundário: Dor Abdominal/fisiopatologia
Animais
Vias Autônomas/crescimento & desenvolvimento
Vias Autônomas/fisiopatologia
Colo/fisiopatologia
Trânsito Gastrointestinal/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Tecido Nervoso/crescimento & desenvolvimento
Tecido Nervoso/fisiopatologia
Dor Pós-Operatória/fisiopatologia
Pelve/fisiopatologia
Pelve/cirurgia
Próstata/inervação
Recuperação de Função Fisiológica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE



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