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[PMID]:28279448
[Au] Autor:Correa ME; Côté AM; De Silva DA; Wang L; Packianathan P; von Dadelszen P; Magee LA
[Ad] Endereço:Department of Obstetrics and Gynaecology, University of British Columbia, 4500 Oak Street, Vancouver, BC V6H 3N1, Canada; Department of Obstetrics and Gynaecology, Universidad Austral de Chile, Simpson 850, Valdivia, Chile.
[Ti] Título:Visual or automated dipstick testing for proteinuria in pregnancy?
[So] Source:Pregnancy Hypertens;7:50-53, 2017 Jan.
[Is] ISSN:2210-7797
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To compare the Multistix 10SG/visual-read with two automated methods (Multistix 10SG/Clinitek 50 and Chemstrip 10A/Urisys 1100) to detect significant proteinuria among high-risk pregnant women. STUDY DESIGN: Prospective cohort study at British Columbia Women's Hospital & Health Centre, Vancouver, Canada. MAIN OUTCOME MEASURES: Diagnostic accuracy determined by sensitivity, specificity, positive and negative likelihood ratios (LR+ and LR-). RESULTS: 303 (89.6%) of 338 women had a urine sample tested by all three dipstick methods. 196 samples (64.7%) were collected in the morning (subsequent to their first void) and from outpatients. 107 samples (35.3%) were from inpatients at various times throughout the day. A PrCr ⩾30mg/mmol was present in 46 (15.2%) samples. The sensitivity for proteinuria was higher with Multistix 10SG/Clinitek 50 (65.2%) than with Multistix 10SG/visual-read (41.3%, p<0.001) or Chemstrip 10A/Urisys 1100 (54.3%, p=0.06). Specificity was >90% for all methods studied, although it was highest for Multistix 10SG/visual-read (98.4%) compared with either Multistix 10SG/Clinitek 50 (92.6%, p<0.001) or Chemstrip 10A/Urisys 1100 (95.7%, p=0.04). For all methods, LR+ was good-excellent (>5), but LR- poor-fair (>0.20). 29 samples were discordant for proteinuria between methods. 28/29 women had negative proteinuria by Multistix 10SG/visual-read, but at least 1+ proteinuria by an automated method; 17/28 were false positives and 11/28 true positives. CONCLUSIONS: Automated dipstick methods are more sensitive than visual urinalysis for proteinuria, but test performance is still only poor-fair as a 'rule-out' test for proteinuria. Whether the enhanced sensitivity would be worth the false positives, cost, and personnel training remains to be determined for detection of low-level proteinuria in pregnancy.
[Mh] Termos MeSH primário: Proteinúria/urina
Fitas Reagentes
[Mh] Termos MeSH secundário: Autoanálise
Estudos de Coortes
Reações Falso-Positivas
Feminino
Seres Humanos
Pré-Eclâmpsia/urina
Gravidez
Estudos Prospectivos
Proteinúria/diagnóstico
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reagent Strips)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE


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[PMID]:27383489
[Au] Autor:Shukla S; Kim M
[Ad] Endereço:Department of Food Science and Technology, Yeungnam University, Gyeongsan-si, Gyeongsangbuk, Republic of Korea.
[Ti] Título:Short Communication - Determination of amino acid and free sugar contents in Korean traditional fermented soybean products of Doenjang and evaluation of their sensory attributes.
[So] Source:Pak J Pharm Sci;29(3 Suppl):1101-7, 2016 May.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:This study quantitatively determined contents of amino acids and free sugars in soybean fermented samples (Doenjang) using amino acid autoanalyzer and HPLC, respectively. As a result, hreonine, serine, glycine, alanine and lysine categorized as total sweet amino acids and free sugars (sucrose, maltose, glucose, galactose and fructose) were found in the range of 32.7 to 117.1 and 5.85 to 23.66mg/100 g, respectively. However, average of total sugar content was noted to be 71.63mg%. Three samples possessing the best sensory characteristics were selected for further study. The mean values of four sensory attributes such as sweet, sour, savory and bitter tastes of Doenjang samples were found to be 709.53, 210.25, 241.90 and 276.05. These findings confirm nutritive properties of fermented food products of Doenjang with improved quality and utilization.
[Mh] Termos MeSH primário: Aminoácidos/análise
Carboidratos/análise
Fermentação
Alimentos de Soja/análise
Percepção Gustatória
Paladar
[Mh] Termos MeSH secundário: Adulto
Autoanálise
Cromatografia Líquida de Alta Pressão
Feminino
Seres Humanos
Masculino
Meia-Idade
República da Coreia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Carbohydrates)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE


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[PMID]:27358150
[Au] Autor:Zhao X; Wang XF; Wang JB; Lu XJ; Zhao YW; Li CB; Wang BH; Wei J; Guo P; Xiao JP; Wang JH; Yang XL
[Ad] Endereço:Department of Laboratory Medicine, Beijing Hospital, Ministry of Health, Dongdan, Dongcheng District, Beijing, China.
[Ti] Título:Multicenter study of autoverification methods of hematology analysis.
[So] Source:J Biol Regul Homeost Agents;30(2):571-7, 2016 Apr-Jun.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:This study was designed to establish and validate a set of autoverification methods for hematology analysis. One thousand and twenty-four samples were selected from Shanghai Ruijin Hospital and 999 from Beijing Hospital, China. False positive, false negative and autoverification pass rates were verified and the rules were then adjusted and confirmed according to the verification results. After confirmation, at least 10,000 sample cases were selected from Shanghai Ruijin Hospital, Beijing Hospital and China Armed Police General Hospital and checked automatically. The differences in the autoverification pass rate and average report delivery time before and after the application of the autoverification methods were compared between the three hospitals. Preliminary validation results showed that the false negative rates of the Shanghai Ruijin Hospital and Beijing Hospital were less than 2%. The false positive rates of these two hospitals were high, close to 18%. After rule adjustment, the false negative rate was basically the same as before adjustment, but the false positive rate declined obviously while the pass rate of autoverification improved significantly. The autoverification pass rates of the three hospitals were 76.4%, 85.1% and 84.2%, respectively. The turnover time (TAT, time from receipt of sample to report of the result) of the three hospitals decreased by 4.1 min, 8.8 min and 10.2 min, respectively. Autoverification systems using a Mindray BC-6800 auto hematology analyzer and labXpert were confirmed as being effective in reducing TAT and enhancing working efficiency on the premise of ensuring low false negative rate.
[Mh] Termos MeSH primário: Testes Hematológicos/métodos
[Mh] Termos MeSH secundário: Autoanálise
Seres Humanos
Laboratórios Hospitalares
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160630
[Lr] Data última revisão:
160630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE


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[PMID]:27085000
[Au] Autor:Froment J; Thomas KV; Tollefsen KE
[Ad] Endereço:Norwegian Institute for Water Research (NIVA), Gaustadalléen 21, N-0349 Oslo, Norway; Department of Chemistry, University of Oslo (UiO), PO Box 1033, Blindern, N-0316 Oslo, Norway. Electronic address: jfr@niva.no.
[Ti] Título:Automated high-throughput in vitro screening of the acetylcholine esterase inhibiting potential of environmental samples, mixtures and single compounds.
[So] Source:Ecotoxicol Environ Saf;130:74-80, 2016 Aug.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A high-throughput and automated assay for testing the presence of acetylcholine esterase (AChE) inhibiting compounds was developed, validated and applied to screen different types of environmental samples. Automation involved using the assay in 96-well plates and adapting it for the use with an automated workstation. Validation was performed by comparing the results of the automated assay with that of a previously validated and standardised assay for two known AChE inhibitors (paraoxon and dichlorvos). The results show that the assay provides similar concentration-response curves (CRCs) when run according to the manual and automated protocol. Automation of the assay resulted in a reduction in assay run time as well as in intra- and inter-assay variations. High-quality CRCs were obtained for both of the model AChE inhibitors (dichlorvos IC50=120µM and paraoxon IC50=0.56µM) when tested alone. The effect of co-exposure of an equipotent binary mixture of the two chemicals were consistent with predictions of additivity and best described by the concentration addition model for combined toxicity. Extracts of different environmental samples (landfill leachate, wastewater treatment plant effluent, and road tunnel construction run-off) were then screened for AChE inhibiting activity using the automated bioassay, with only landfill leachate shown to contain potential AChE inhibitors. Potential uses and limitations of the assay were discussed based on the present results.
[Mh] Termos MeSH primário: Inibidores da Colinesterase/análise
Diclorvós/análise
Ensaios de Triagem em Larga Escala
Paraoxon/análise
[Mh] Termos MeSH secundário: Autoanálise
Bioensaio
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Cholinesterase Inhibitors); 0 (Water Pollutants, Chemical); 7U370BPS14 (Dichlorvos); Q9CX8P80JW (Paraoxon)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160417
[St] Status:MEDLINE


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[PMID]:27069030
[Au] Autor:Wesarachkitti B; Khejonnit V; Pratumvinit B; Reesukumal K; Meepanya S; Pattanavin C; Wongkrajang P
[Ad] Endereço:Department of Clinical Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
[Ti] Título:Performance Evaluation and Comparison of the Fully Automated Urinalysis Analyzers UX-2000 and Cobas 6500.
[So] Source:Lab Med;47(2):124-33, 2016 May.
[Is] ISSN:1943-7730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate and compare the performances of the automated urinalysis devices UX-2000 and Cobas 6500. METHOD: A total of 258 urine specimens were collected from the routine specimen workload. We analyzed all specimens on both automated instruments and recorded the turnaround time from each method. Physical, chemical, and sedimentary urine components were compared between the automated and the manual method for each analyzer. RESULTS: The correlation of urine physical/chemical properties between the 2 instruments was excellent. The Cobas 6500 instrument demonstrated a higher level of agreement for red blood cells (Cobas 6500:R= 0.94; UX-2000:R= 0.78) and white blood cells (Cobas 6500:R= 0.95; UX-2000:R= 0.85). The UX-2000 demonstrated higher sensitivity for small round cells, hyaline casts, pathological casts, and bacteria. The median turnaround time was 1.5 minutes and 8.5 minutes for the Cobas 6500 and UX-2000, respectively. CONCLUSIONS: The 2 devices showed similar performance in technical evaluation; they each reduce workload and increase time saving. However, manual examination by technicians is recommended for pathological specimens.
[Mh] Termos MeSH primário: Eritrócitos/patologia
Leucócitos/patologia
Urinálise/instrumentação
[Mh] Termos MeSH secundário: Autoanálise
Contagem de Células
Seres Humanos
Microscopia
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161231
[Lr] Data última revisão:
161231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160413
[St] Status:MEDLINE
[do] DOI:10.1093/labmed/lmw002


  6 / 10754 MEDLINE  
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[PMID]:27069029
[Au] Autor:Pocino K; Molinario R; Manieri R; Bianucci L; Capoluongo E
[Ad] Endereço:Institute of Clinical Biochemistry, Catholic University of Sacred Heart, Rome, Italy and krizia.pocino@gmail.com.
[Ti] Título:The Hemo One Autoanalyzer for Glycated Hemoglobin Assay.
[So] Source:Lab Med;47(2):119-23, 2016 May.
[Is] ISSN:1943-7730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Glycated hemoglobin (HbA1c) is widely used as a clinical marker of long-term blood glucose concentration in patients with diabetes. The clinical laboratory plays a vital role in the diagnosis and management of diabetes. Many methods for the measurement of HbA1c have been developed based on different analytical principles, often causing discordant results. For this reason, the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) established a reference method for HbA1c assay, namely, high-performance liquid chromatography-mass spectrometry/capillary electrophoresis (HPLC-MS/CE). OBJECTIVE: In order to evaluate in parallel 2 different routine methods, namely, ion-exchange HPLC and immunoturbidimetry. METHODS: For our comparison study, we used the Tosoh G8 HPLC analyzer and the Hemo One autoanalyzer system to test 100 blood specimens for HbA1c concentration, the values of which ranged from 4.3% (23.5 mmol/mol) to 14.7% (137 mmol/mol). RESULTS: Concordance between HPLC and the immunoturbidimetric method revealed perfect agreement with a Kappa value of 0.828. CONCLUSIONS: Our results confirm the validity of the immunoturbidimetric method compared with the reference method. Our findings highlight that these 2 methods are equivalent for the diagnosis and therapeutic monitoring of diabetes.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Diabetes Mellitus/diagnóstico
Hemoglobina A Glicada/metabolismo
Nefelometria e Turbidimetria/instrumentação
[Mh] Termos MeSH secundário: Autoanálise
Cromatografia Líquida de Alta Pressão
Eletroforese Capilar
Hemoglobina A Glicada/química
Seres Humanos
Padrões de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glycated Hemoglobin A)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160413
[St] Status:MEDLINE
[do] DOI:10.1093/labmed/lmv034


  7 / 10754 MEDLINE  
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[PMID]:26930553
[Au] Autor:Zhou L; Jones C; Gibani MM; Dobinson H; Thomaides-Brears H; Shrestha S; Blohmke CJ; Darton TC; Pollard AJ
[Ad] Endereço:Oxford Vaccine Group, Department of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom.
[Ti] Título:Development and Evaluation of a Blood Culture PCR Assay for Rapid Detection of Salmonella Paratyphi A in Clinical Samples.
[So] Source:PLoS One;11(3):e0150576, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enteric fever remains an important cause of morbidity in many low-income countries and Salmonella Paratyphi A has emerged as the aetiological agent in an increasing proportion of cases. Lack of adequate diagnostics hinders early diagnosis and prompt treatment of both typhoid and paratyphoid but development of assays to identify paratyphoid has been particularly neglected. Here we describe the development of a rapid and sensitive blood culture PCR method for detection of Salmonella Paratyphi A from blood, potentially allowing for appropriate diagnosis and antimicrobial treatment to be initiated on the same day. METHODS: Venous blood samples from volunteers experimentally challenged orally with Salmonella Paratyphi A, who subsequently developed paratyphoid, were taken on the day of diagnosis; 10 ml for quantitative blood culture and automated blood culture, and 5 ml for blood culture PCR. In the latter assay, bacteria were grown in tryptone soy broth containing 2.4% ox bile and micrococcal nuclease for 5 hours (37°C) before bacterial DNA was isolated for PCR detection targeting the fliC-a gene of Salmonella Paratyphi A. RESULTS: An optimized broth containing 2.4% ox bile and micrococcal nuclease, as well as a PCR test was developed for a blood culture PCR assay of Salmonella Paratyphi A. The volunteers diagnosed with paratyphoid had a median bacterial burden of 1 (range 0.1-6.9) CFU/ml blood. All the blood culture PCR positive cases where a positive bacterial growth was shown by quantitative blood culture had a bacterial burden of ≥ 0.3 CFU/ ml blood. The blood culture PCR assay identified an equal number of positive cases as automated blood culture at higher bacterial loads (≥0.3 CFU/ml blood), but utilized only half the volume of specimens. CONCLUSIONS: The blood culture PCR method for detection of Salmonella Paratyphi A can be completed within 9 hours and offers the potential for same-day diagnosis of enteric fever. Using 5 ml blood, it exhibited a lower limit of detection equal to 0.3 CFU/ml blood, and it performed at least as well as automated blood culture at higher bacterial loads (≥0.3 CFU/ml blood) of clinical specimens despite using half the volume of blood. The findings warrant its further study in endemic populations with a potential use as a novel diagnostic which fills the present gap of paratyphoid diagnostics.
[Mh] Termos MeSH primário: Febre Paratifoide/diagnóstico
Reação em Cadeia da Polimerase/métodos
Salmonella paratyphi A
[Mh] Termos MeSH secundário: Autoanálise/métodos
DNA Bacteriano/genética
Seres Humanos
Febre Paratifoide/sangue
Salmonella paratyphi A/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0150576


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[PMID]:26905347
[Au] Autor:Yilmaz S; Uysal HB; Avcil M; Yilmaz M; Dagli B; Bakis M; Ömürlü IK
[Ad] Endereço:Department of Anesthesiology and Reanimation, Adnan Menderes University School of Medicine, Aydin, Turkey. E-mail. dr_snnylmz@hotmail.com.
[Ti] Título:Comparison of different methods for measurement of electrolytes in patients admitted to the intensive care unit.
[So] Source:Saudi Med J;37(3):262-7, 2016 Mar.
[Is] ISSN:0379-5284
[Cp] País de publicação:Saudi Arabia
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To investigate whether electrolyte levels measured by using blood gas analyzers (ABG) and auto-analyzers (AA) are equivalent and can be used interchangeably. METHODS: This observational prospective study was conducted in 100 patients admitted to the Intensive Care Unit, Adnan Menderes University School of Medicine, Aydin, Turkey, between March and August 2014. Samples for both AA and ABG analyzers were collected simultaneously from invasive arterial catheters of patients. The electrolyte levels were measured by using 2 methods. RESULTS: The mean sodium level measured by ABG was 136.1 ± 6.3 mmol/L and 137.8 ± 5.4 mmol/L for AA (p=0.001). The Pearson's correlation coefficient was 0.561 (p less than 0.001). The Bland-Altman 95% limits of agreement were -9.4 to 12.6 mmol/L. The mean potassium levels measured by ABG was 3.4 ± 0.7 mmol/L and AA was 3.8 ± 0.7 mmol/L (p=0.001). The Bland-Altman comparison limits were -0.58 to 1.24 and the associated Pearson's correlation coefficient was 0.812 (p less than 0.001). CONCLUSION: The results of the 2 analyzing methods, in terms of sodium, were not equivalent and could not be used interchangeably. However, according to the statistical analyses results, by including, but not blindly trusting these findings, urgent and vital decisions could be made by the potassium levels obtained from the BGA, but a simultaneous follow-up sample had to be sent to the central laboratory for confirmation.
[Mh] Termos MeSH primário: Análise Química do Sangue/métodos
Potássio/sangue
Sódio/sangue
Desequilíbrio Hidroeletrolítico/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Autoanálise/instrumentação
Gasometria/instrumentação
Estudos de Coortes
Feminino
Hospitalização
Seres Humanos
Unidades de Terapia Intensiva
Masculino
Meia-Idade
Estudos Prospectivos
Desequilíbrio Hidroeletrolítico/sangue
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
9NEZ333N27 (Sodium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.15537/smj.2016.3.13539


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[PMID]:26792376
[Au] Autor:Kimura T; Kuwata H; Miyauchi K; Katayama Y; Kayahara N; Sugiuchi H; Matsushima K; Kondo Y; Ishitsuka Y; Irikura M; Irie T
[Ad] Endereço:Research & Development Department, Kyowa Medex, Tokyo 104-6004, Japan. Electronic address: takehide.kimura@kyowa-kirin.co.jp.
[Ti] Título:An enzyme combination assay for serum sphingomyelin: Improved specificity through avoiding the interference with lysophosphatidylcholine.
[So] Source:Anal Biochem;498:29-36, 2016 Apr 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Serum sphingomyelin (SM) has predictive value in the development of atherosclerosis. Furthermore, SM plays important roles in cell membrane structure, signal transduction pathways, and lipid raft formation. A convenient enzymatic method for SM is available for routine laboratory practice, but the enzyme specificity is not sufficient because of nonspecific reactions with lysophosphatidylcholine (LPC). Based on the differential specificity of selected enzymes toward choline-containing phospholipids, a two-step assay for measuring SM was constructed and its performance was evaluated using sera from healthy individuals on a Hitachi 7170 autoanalyzer. Results from this assay were highly correlated with theoretical serum SM concentrations estimated by subtracting phosphatidylcholine (PC) and LPC concentrations from that of total phospholipids determined using previously established methods. There was a good correlation between the results of SM assayed by the proposed method and the existing enzymatic method in sera from healthy individuals. Moreover, the proposed method was superior to the existing method in preventing nonspecific reactions with LPC present in sera. The proposed method does not require any pretreatment, uses 2.5 µl of serum samples, and requires only 10 min on an autoanalyzer. This high-throughput method can measure serum SM with sufficient specificity for clinical purposes and is applicable in routine laboratory practice.
[Mh] Termos MeSH primário: Autoanálise
Ensaios Enzimáticos
Lisofosfatidilcolinas/química
Esfingomielina Fosfodiesterase/metabolismo
Esfingomielinas/sangue
[Mh] Termos MeSH secundário: Adulto
Artefatos
Membrana Celular/química
Membrana Celular/metabolismo
Feminino
Seres Humanos
Lisofosfatidilcolinas/metabolismo
Masculino
Meia-Idade
Esfingomielinas/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lysophosphatidylcholines); 0 (Sphingomyelins); EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160122
[St] Status:MEDLINE


  10 / 10754 MEDLINE  
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[PMID]:26526598
[Au] Autor:Tormey WP; O'Hagan C
[Ad] Endereço:Department of Chemical Pathology, Beaumont Hospital, Dublin, Ireland ; Athlone Institute of Technology, Athlone, Ireland ; Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland.
[Ti] Título:Cerebrospinal fluid protein and glucose examinations and tuberculosis:Will laboratory safety regulations force a change of practice?
[So] Source:Biochem Med (Zagreb);25(3):359-62, 2015.
[Is] ISSN:1330-0962
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:Cerebrospinal fluid (CSF) protein and glucose examinations are usually performed in chemical pathology departments on autoanalysers. Tuberculosis (TB) is a group 3 biological agent under Directive 2000/54/EC of the European Parliament but in the biochemistry laboratory, no extra precautions are taken in its analysis in possible TB cases. The issue of laboratory practice and safety in the biochemical analyses of CSF specimens, when tuberculosis infection is in question is addressed in the context of ambiguity in the implementation of current national and international health and safety regulations. Additional protective measures for laboratory staff during the analysis of CSF TB samples should force a change in current laboratory practice and become a regulatory issue under ISO 15189. Annual Mantoux skin test or an interferon-γ release assay for TB should be mandatory for relevant staff. This manuscript addresses the issue of biochemistry laboratory practice and safety in the biochemical analyses of CSF specimens when tuberculosis infection is in question in the context of the ambiguity of statutory health and safety regulations.
[Mh] Termos MeSH primário: Proteínas do Líquido Cefalorraquidiano/análise
Contenção de Riscos Biológicos/normas
Glucose/líquido cefalorraquidiano
Infecção Laboratorial/prevenção & controle
Saúde do Trabalhador/legislação & jurisprudência
Prática Profissional/normas
Gestão da Segurança/legislação & jurisprudência
Tuberculose/líquido cefalorraquidiano
[Mh] Termos MeSH secundário: Autoanálise/instrumentação
Automação Laboratorial/instrumentação
Líquido Cefalorraquidiano/química
Líquido Cefalorraquidiano/microbiologia
Seres Humanos
Controle de Infecções/normas
Irlanda
Infecção Laboratorial/epidemiologia
Infecção Laboratorial/transmissão
Pessoal de Laboratório Médico
Saúde do Trabalhador/normas
Prática Profissional/tendências
Gestão da Segurança/normas
Manejo de Espécimes/normas
Tuberculose/epidemiologia
Tuberculose/prevenção & controle
Tuberculose/transmissão
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cerebrospinal Fluid Proteins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151104
[St] Status:MEDLINE
[do] DOI:10.11613/BM.2015.035



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