Base de dados : MEDLINE
Pesquisa : E05.064 [Categoria DeCS]
Referências encontradas : 1721 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 173 ir para página                         

  1 / 1721 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27779124
[Au] Autor:Kim Y; Bae SK; Cheng T; Tao C; Ge Y; Chapman AB; Torres VE; Yu AS; Mrug M; Bennett WM; Flessner MF; Landsittel DP; Bae KT
[Ad] Endereço:Department of Radiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
[Ti] Título:Automated segmentation of liver and liver cysts from bounded abdominal MR images in patients with autosomal dominant polycystic kidney disease.
[So] Source:Phys Med Biol;61(22):7864-7880, 2016 Nov 21.
[Is] ISSN:1361-6560
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Liver and liver cyst volume measurements are important quantitative imaging biomarkers for assessment of disease progression in autosomal dominant polycystic kidney disease (ADPKD) and polycystic liver disease (PLD). To date, no study has presented automated segmentation and volumetric computation of liver and liver cysts in these populations. In this paper, we proposed an automated segmentation framework for liver and liver cysts from bounded abdominal MR images in patients with ADPKD. To model the shape and variations in ADPKD livers, the spatial prior probability map (SPPM) of liver location and the tissue prior probability maps (TPPMs) of liver parenchymal tissue intensity and cyst morphology were generated. Formulated within a three-dimensional level set framework, the TPPMs successfully captured liver parenchymal tissues and cysts, while the SPPM globally constrained the initial surfaces of the liver into the desired boundary. Liver cysts were extracted by combined operations of the TPPMs, thresholding, and false positive reduction based on spatial prior knowledge of kidney cysts and distance map. With cross-validation for the liver segmentation, the agreement between the radiology expert and the proposed method was 84% for shape congruence and 91% for volume measurement assessed by the intra-class correlation coefficient (ICC). For the liver cyst segmentation, the agreement between the reference method and the proposed method was ICC = 0.91 for cyst volumes and ICC = 0.94 for % cyst-to-liver volume.
[Mh] Termos MeSH primário: Abdome/patologia
Algoritmos
Cistos/patologia
Interpretação de Imagem Assistida por Computador/métodos
Hepatopatias/patologia
Fígado/patologia
Imagem por Ressonância Magnética/métodos
Rim Policístico Autossômico Dominante/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Automação Laboratorial
Progressão da Doença
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161027
[St] Status:MEDLINE


  2 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27775692
[Au] Autor:Aeffner F; Martin NT; Peljto M; Black JC; Major JK; Jangani M; Ports MO; Krueger JS; Young GD
[Ad] Endereço:Flagship Biosciences Inc., Westminster, CO, USA.
[Ti] Título:Quantitative assessment of pancreatic cancer precursor lesions in IHC-stained tissue with a tissue image analysis platform.
[So] Source:Lab Invest;96(12):1327-1336, 2016 12.
[Is] ISSN:1530-0307
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tissue image analysis (tIA) is emerging as a powerful tool for quantifying biomarker expression and distribution in complex diseases and tissues. Pancreatic ductal adenocarcinoma (PDAC) develops in a highly complex and heterogeneous tissue environment and, generally, has a very poor prognosis. Early detection of PDAC is confounded by limited knowledge of the pre-neoplastic disease stages and limited methods to quantitatively assess disease heterogeneity. We sought to develop a tIA approach to assess the most common PDAC precursor lesions, pancreatic intraepithelial neoplasia (PanIN), in tissues from Kras ; Trp53 ; Pdx-Cre (KPC) mice, a validated model of PDAC development. tIA profiling of training regions of PanIN and tumor microenvironment (TME) cells was utilized to guide identification of PanIN/TME tissue compartment stratification criteria. A custom CellMap algorithm implementing these criteria was applied to whole-slide images of KPC mice pancreata sections to quantify p53 and Ki-67 biomarker staining in each tissue compartment as a proof-of-concept for the algorithm platform. The algorithm robustly identified a higher percentage of p53-positive cells in PanIN lesions relative to the TME, whereas no difference was observed for Ki-67. Ki-67 expression was also quantified in a human pancreatic tissue sample available to demonstrate the translatability of the CellMap algorithm to human samples. Together, our data demonstrated the utility of CellMap to enable objective and quantitative assessments, across entire tissue sections, of PDAC precursor lesions in preclinical and clinical models of this disease to support efforts leading to novel insights into disease progression, diagnostic markers, and potential therapeutic targets.
[Mh] Termos MeSH primário: Adenocarcinoma in Situ/diagnóstico
Carcinoma Ductal Pancreático/diagnóstico
Pâncreas/patologia
Neoplasias Pancreáticas/diagnóstico
Lesões Pré-Cancerosas/diagnóstico
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Adenocarcinoma in Situ/diagnóstico por imagem
Adenocarcinoma in Situ/metabolismo
Adenocarcinoma in Situ/patologia
Algoritmos
Animais
Automação Laboratorial
Carcinoma Ductal Pancreático/diagnóstico por imagem
Carcinoma Ductal Pancreático/metabolismo
Carcinoma Ductal Pancreático/patologia
Cruzamentos Genéticos
Modelos Animais de Doenças
Detecção Precoce de Câncer/métodos
Seres Humanos
Processamento de Imagem Assistida por Computador
Imuno-Histoquímica
Antígeno Ki-67/metabolismo
Camundongos Mutantes
Camundongos Transgênicos
Pâncreas/metabolismo
Neoplasias Pancreáticas/diagnóstico por imagem
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
Lesões Pré-Cancerosas/diagnóstico por imagem
Lesões Pré-Cancerosas/metabolismo
Lesões Pré-Cancerosas/patologia
Software
Organismos Livres de Patógenos Específicos
Bancos de Tecidos
Ultrassonografia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ki-67 Antigen); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1038/labinvest.2016.111


  3 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29241086
[Au] Autor:Hameed R; Huddleston J; Ignatova S
[Ad] Endereço:Advanced Bioprocessing Centre, Institute of Environment, Health and Societies, College of Engineering, Design and Physical Sciences, Brunel University London, Uxbridge, UB8 3PH, UK.
[Ti] Título:Practical aspects of the automated preparation of aqueous two phase systems for the analysis of biological macromolecules.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:60-68, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A robust strategy for the automated preparation of aqueous two-phase systems (ATPS) using a liquid handling sample processor was developed using gravimetric methods: to determine the accuracy of preparation. The major robotic control parameters requiring adjustment were; speed of aspiration and dispense; delay times following aspiration and dispense alongside measures to control cross-contamination during phase sampling. In general mixture compositions of both polymer/polymer and polymer/salt mixtures could be prepared with a target bias accuracy of less than 5%. However, we found that the bias accuracy with which systems of defined TLL and MR could be constructed was highly dependent on the tie line length of the ATPS and the geometrical form of the ATPS co-existence curve. For systems with a very low degree of curvature (PEG/salt systems here) increases in bias (accuracy) are appreciable at relatively long tie line lengths. Where the degree of curvature is more pronounced (PEG/dextran systems) closer approach to the critical point was possible without major effect on bias/accuracy. Application of the strategy to the measurement of the partitioning of phosphorylated and dephosphorylated forms of the model protein ovalbumin are reported. Differences in partition of phosphorylated (native) forms and dephosphorylated forms could be demonstrated. In a PEG/salt system this was manifest as a substantial decrease in solubility based on overall protein recovery derived from accurate knowledge of the system mass ratio. In a PEG/dextran system differences in partition coefficient could be demonstrated between phosphorylated and dephosphorylated forms.
[Mh] Termos MeSH primário: Automação Laboratorial/métodos
Cromatografia Líquida/métodos
Extração Líquido-Líquido/métodos
Modelos Químicos
[Mh] Termos MeSH secundário: Ovalbumina/análise
Ovalbumina/química
Ovalbumina/isolamento & purificação
Isoformas de Proteínas/análise
Isoformas de Proteínas/química
Isoformas de Proteínas/isolamento & purificação
Proteínas Recombinantes/análise
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Robótica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Isoforms); 0 (Recombinant Proteins); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  4 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29304138
[Au] Autor:Thakur SS; Li H; Chan AMY; Tudor R; Bigras G; Morris D; Enwere EK; Yang H
[Ad] Endereço:Department of Oncology, University of Calgary, Calgary, Alberta, Canada.
[Ti] Título:The use of automated Ki67 analysis to predict Oncotype DX risk-of-recurrence categories in early-stage breast cancer.
[So] Source:PLoS One;13(1):e0188983, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ki67 is a commonly used marker of cancer cell proliferation, and has significant prognostic value in breast cancer. In spite of its clinical importance, assessment of Ki67 remains a challenge, as current manual scoring methods have high inter- and intra-user variability. A major reason for this variability is selection bias, in that different observers will score different regions of the same tumor. Here, we developed an automated Ki67 scoring method that eliminates selection bias, by using whole-slide analysis to identify and score the tumor regions with the highest proliferative rates. The Ki67 indices calculated using this method were highly concordant with manual scoring by a pathologist (Pearson's r = 0.909) and between users (Pearson's r = 0.984). We assessed the clinical validity of this method by scoring Ki67 from 328 whole-slide sections of resected early-stage, hormone receptor-positive, human epidermal growth factor receptor 2-negative breast cancer. All patients had Oncotype DX testing performed (Genomic Health) and available Recurrence Scores. High Ki67 indices correlated significantly with several clinico-pathological correlates, including higher tumor grade (1 versus 3, P<0.001), higher mitotic score (1 versus 3, P<0.001), and lower Allred scores for estrogen and progesterone receptors (P = 0.002, 0.008). High Ki67 indices were also significantly correlated with higher Oncotype DX risk-of-recurrence group (low versus high, P<0.001). Ki67 index was the major contributor to a machine learning model which, when trained solely on clinico-pathological data and Ki67 scores, identified Oncotype DX high- and low-risk patients with 97% accuracy, 98% sensitivity and 80% specificity. Automated scoring of Ki67 can thus successfully address issues of consistency, reproducibility and accuracy, in a manner that integrates readily into the workflow of a pathology laboratory. Furthermore, automated Ki67 scores contribute significantly to models that predict risk of recurrence in breast cancer.
[Mh] Termos MeSH primário: Neoplasias da Mama/química
Processamento de Imagem Assistida por Computador/métodos
Antígeno Ki-67/análise
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Automação Laboratorial/métodos
Automação Laboratorial/estatística & dados numéricos
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Proliferação Celular
Estudos de Coortes
Feminino
Seres Humanos
Processamento de Imagem Assistida por Computador/estatística & dados numéricos
Imuno-Histoquímica/métodos
Imuno-Histoquímica/estatística & dados numéricos
Aprendizado de Máquina
Meia-Idade
Recidiva Local de Neoplasia/química
Recidiva Local de Neoplasia/patologia
Prognóstico
Receptor ErbB-2/metabolismo
Receptores Estrogênicos/metabolismo
Receptores de Progesterona/metabolismo
Reprodutibilidade dos Testes
Estudos Retrospectivos
Fatores de Risco
Viés de Seleção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ki-67 Antigen); 0 (Receptors, Estrogen); 0 (Receptors, Progesterone); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188983


  5 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28985361
[Au] Autor:Bonaldi K; Li Z; Kang SE; Breton G; Pruneda-Paz JL
[Ad] Endereço:Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
[Ti] Título:Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens.
[So] Source:Nucleic Acids Res;45(18):e157, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter.
[Mh] Termos MeSH primário: Genes Reporter
Ensaios de Triagem em Larga Escala/métodos
Luciferases/genética
Técnicas do Sistema de Duplo-Híbrido
[Mh] Termos MeSH secundário: Automação Laboratorial
Sítios de Ligação/genética
Regulação da Expressão Gênica/genética
Técnicas de Transferência de Genes
Organismos Geneticamente Modificados
Regiões Promotoras Genéticas
Ligação Proteica
Elementos Reguladores de Transcrição/genética
Saccharomyces cerevisiae
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Transcription Factors); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx682


  6 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28910313
[Au] Autor:Horsch S; Kopczynski D; Kuthe E; Baumbach JI; Rahmann S; Rahnenführer J
[Ad] Endereço:Department of Statistics, TU Dortmund University, Dortmund, Germany.
[Ti] Título:A detailed comparison of analysis processes for MCC-IMS data in disease classification-Automated methods can replace manual peak annotations.
[So] Source:PLoS One;12(9):e0184321, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MOTIVATION: Disease classification from molecular measurements typically requires an analysis pipeline from raw noisy measurements to final classification results. Multi capillary column-ion mobility spectrometry (MCC-IMS) is a promising technology for the detection of volatile organic compounds in the air of exhaled breath. From raw measurements, the peak regions representing the compounds have to be identified, quantified, and clustered across different experiments. Currently, several steps of this analysis process require manual intervention of human experts. Our goal is to identify a fully automatic pipeline that yields competitive disease classification results compared to an established but subjective and tedious semi-manual process. METHOD: We combine a large number of modern methods for peak detection, peak clustering, and multivariate classification into analysis pipelines for raw MCC-IMS data. We evaluate all combinations on three different real datasets in an unbiased cross-validation setting. We determine which specific algorithmic combinations lead to high AUC values in disease classifications across the different medical application scenarios. RESULTS: The best fully automated analysis process achieves even better classification results than the established manual process. The best algorithms for the three analysis steps are (i) SGLTR (Savitzky-Golay Laplace-operator filter thresholding regions) and LM (Local Maxima) for automated peak identification, (ii) EM clustering (Expectation Maximization) and DBSCAN (Density-Based Spatial Clustering of Applications with Noise) for the clustering step and (iii) RF (Random Forest) for multivariate classification. Thus, automated methods can replace the manual steps in the analysis process to enable an unbiased high throughput use of the technology.
[Mh] Termos MeSH primário: Automação Laboratorial/métodos
Curadoria de Dados
Modelos Teóricos
Análise Espectral
[Mh] Termos MeSH secundário: Testes Respiratórios/instrumentação
Testes Respiratórios/métodos
Seres Humanos
Análise Espectral/instrumentação
Análise Espectral/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184321


  7 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28802157
[Au] Autor:Americo JL; Earl PL; Moss B
[Ad] Endereço:Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, United States.
[Ti] Título:Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses.
[So] Source:Virology;511:19-22, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Droplet digital polymerase chain reaction (ddPCR) was adapted for quantifying the number of orthopoxviral genomes in purified virus samples, infected cell lysates and tissues of infected animals. In contrast to the more commonly used qPCR, the newer ddPCR provides absolute numbers of DNA copies in samples without need for standard curves and has the ability to detect rare mutants in a population. The genome/infectious unit ratio for several sucrose gradient-purified orthopoxviruses varied from 5 to 10, which correlated well with values obtained using the Virocyt, a dedicated fluorescence flow cytometer. By employing a nuclease step to digest unencapsulated DNA, the genome/infectious unit ratios of virus in crude cell lysates approached that of purified virus particles. The speed, accuracy, sensitivity, and dynamic range of less than one to millions of infectious units in a sample make this semi-automated method well suited to a variety of laboratory, animal and clinical studies.
[Mh] Termos MeSH primário: Orthopoxvirus/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Carga Viral/métodos
[Mh] Termos MeSH secundário: Animais
Automação Laboratorial
Orthopoxvirus/genética
Sensibilidade e Especificidade
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE


  8 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28759622
[Au] Autor:Hardie DR; Korsman SN; Hsiao NY; Morobadi MD; Vawda S; Goedhals D
[Ad] Endereço:Division of Virology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
[Ti] Título:Contamination with HIV antibody may be responsible for false positive results in specimens tested on automated platforms running HIV 4th generation assays in a region of high HIV prevalence.
[So] Source:PLoS One;12(7):e0182167, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: In South Africa where the prevalence of HIV infection is very high, 4th generation HIV antibody/p24 antigen combo immunoassays are the tests of choice for laboratory based screening. Testing is usually performed in clinical pathology laboratories on automated analysers. To investigate the cause of false positive results on 4th generation HIV testing platforms in public sector laboratories, the performance of two automated platforms was compared in a clinical pathology setting, firstly on routine diagnostic specimens and secondly on known sero-negative samples. METHODS: Firstly, 1181 routine diagnostic specimens were sequentially tested on Siemens and Roche automated 4th generation platforms. HIV viral load, western blot and follow up testing were used to determine the true status of inconclusive specimens. Subsequently, known HIV seronegative samples from a single donor were repeatedly tested on both platforms and an analyser was tested for surface contamination with HIV positive serum to identify how suspected specimen contamination could be occurring. RESULTS: Serial testing of diagnostic specimens yielded 163 weakly positive or discordant results. Only 3 of 163 were conclusively shown to indicate true HIV infection. Specimen contamination with HIV antibody was suspected, based on the following evidence: the proportion of positive specimens increased on repeated passage through the analysers; viral loads were low or undetectable and western blots negative or indeterminate on problem specimens; screen negative, 2nd test positive specimens tested positive when reanalysed on the screening assay; follow up specimens (where available) were negative. Similarly, an increasing number of known negative specimens became (repeatedly) sero-positive on serial passage through one of the analysers. Internal and external analyser surfaces were contaminated with HIV serum, evidence that sample splashes occur during testing. CONCLUSIONS: Due to the extreme sensitivity of these assays, contamination with minute amounts of HIV antibody can cause a negative sample to test positive. Better contamination control measures are needed on analysers used in clinical pathology environments, especially in regions where HIV sero-prevalence is high.
[Mh] Termos MeSH primário: Sorodiagnóstico da AIDS/normas
Automação Laboratorial/normas
Contaminação de Equipamentos
Infecções por HIV/sangue
[Mh] Termos MeSH secundário: Sorodiagnóstico da AIDS/instrumentação
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Automação Laboratorial/instrumentação
Reações Falso-Positivas
HIV/imunologia
Infecções por HIV/epidemiologia
Seres Humanos
Prevalência
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182167


  9 / 1721 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28700909
[Au] Autor:Feriani L; Juenet M; Fowler CJ; Bruot N; Chioccioli M; Holland SM; Bryant CE; Cicuta P
[Ad] Endereço:Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
[Ti] Título:Assessing the Collective Dynamics of Motile Cilia in Cultures of Human Airway Cells by Multiscale DDM.
[So] Source:Biophys J;113(1):109-119, 2017 Jul 11.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The technique of differential dynamic microscopy is extended here, showing that it can provide a powerful and objective method of video analysis for optical microscopy videos of in vitro samples of live human bronchial epithelial ciliated cells. These cells are multiciliated, with motile cilia that play key physiological roles. It is shown that the ciliary beat frequency can be recovered to match conventional analysis, but in a fully automated fashion. Furthermore, it is shown that the properties of spatial and temporal coherence of cilia beat can be recovered and distinguished, and that if a collective traveling wave (the metachronal wave) is present, this has a distinct signature and its wavelength and direction can be measured.
[Mh] Termos MeSH primário: Brônquios/metabolismo
Cílios/metabolismo
Células Epiteliais/metabolismo
Microscopia de Vídeo/métodos
Mucosa Nasal/metabolismo
[Mh] Termos MeSH secundário: Automação Laboratorial/métodos
Células Cultivadas
Seres Humanos
Imagem Óptica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


  10 / 1721 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28687633
[Au] Autor:Kahaly GJ; Algeciras-Schimnich A; Davis TE; Diana T; Feldkamp J; Karger S; König J; Lupo MA; Raue F; Ringel MD; Sipos JA; Kratzsch J
[Ad] Endereço:Molecular Thyroid Research Laboratory, Department of Medicine I, Johannes Gutenberg University Medical Center, Mainz, Germany; gkahaly@uni-mainz.de.
[Ti] Título:United States and European Multicenter Prospective Study for the Analytical Performance and Clinical Validation of a Novel Sensitive Fully Automated Immunoassay for Calcitonin.
[So] Source:Clin Chem;63(9):1489-1496, 2017 Sep.
[Is] ISSN:1530-8561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The objective of this study is the validation and proof of clinical relevance of a novel electrochemiluminescence immunoassay (ECLIA) for the determination of serum calcitonin (CT) in patients with medullary thyroid carcinoma (MTC) and in different diseases of the thyroid and of calcium homeostasis. METHODS: This was a multicenter prospective study on basal serum CT concentrations performed in 9 US and European referral institutions. In addition, stimulated CT concentrations were measured in 50 healthy volunteers after intravenous calcium administration (2.5 mg/kg bodyweight). RESULTS: In total, 1929 patients and healthy controls were included. Limits of blank, detection, and quantification for the ECLIA were 0.3, 0.5, and 1 ng/L, respectively. Highest intra- and interassay coefficients of variation were 7.4% (CT concentration, 0.8 ng/L) and 7.0% (1.1 ng/L), respectively. Medians (interval) of serum CT concentrations in 783 healthy controls were 0.8 ng/L (<0.5-12.7) and 3 ng/L (<0.5-18) for females and males, respectively (97.5th percentile, 6.8 and 11.6 ng/L, respectively). Diagnostic sensitivity and specificity were 100%/97.1% and 96.2%/96.4%, for female/males, respectively. Patients (male/female) with primary hyperparathyroidism, renal failure, and neuroendocrine tumors showed CT concentrations >97.5th percentile in 33%/4.7%, 18.5%/10%, and 8.3%/12%, females/males, respectively. Peak serum CT concentrations were reached 2 min after calcium administration (161.7 and 111.8 ng/L in males and females, respectively; < 0.001). CONCLUSIONS: Excellent analytical performance, low interindividual variability, and low impact of confounders for increased CT concentrations in non-MTC patients indicate that the investigated assay has appropriate clinical utility. Calcium-stimulated CT results suggest good test applicability owing to low interindividual variability.
[Mh] Termos MeSH primário: Calcitonina/sangue
Imunoensaio/métodos
Imunoensaio/normas
[Mh] Termos MeSH secundário: Adulto
Automação Laboratorial/instrumentação
Automação Laboratorial/normas
Cálcio/administração & dosagem
Europa (Continente)
Feminino
Seres Humanos
Imunoensaio/tendências
Masculino
Estudos Prospectivos
Padrões de Referência
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Estados Unidos
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; MULTICENTER STUDY; VALIDATION STUDIES
[Nm] Nome de substância:
9007-12-9 (Calcitonin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE
[do] DOI:10.1373/clinchem.2016.270009



página 1 de 173 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde