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Pesquisa : E05.114 [Categoria DeCS]
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[PMID]:28314873
[Au] Autor:da Silva RR; Pedezzi R; Souto TB
[Ad] Endereço:Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café, s/n Campus Universitário da USP, Ribeirão Preto, São Paulo, 14040-903, Brazil. rds.roni@yahoo.com.br.
[Ti] Título:Exploring the bioprospecting and biotechnological potential of white-rot and anaerobic Neocallimastigomycota fungi: peptidases, esterases, and lignocellulolytic enzymes.
[So] Source:Appl Microbiol Biotechnol;101(8):3089-3101, 2017 Apr.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Fungi constitute an invaluable natural resource for scientific research, owing to their diversity; they offer a promising alternative for bioprospecting, thus contributing to biotechnological advances. For a long time, extensive information has been exploited and fungal products have been tested as a source of natural compounds. In this context, enzyme production remains a field of interest, since it offers an efficient alternative to the hazardous processes of chemical transformations. Owing to their vast biodiversity and peculiar biochemical characteristics, two fungal categories, white-rot and anaerobic Neocallimastigomycota, have gathered considerable attention for biotechnological applications. These fungi are known for their ability to depolymerize complex molecular structures and are used in degradation of lignocellulosic biomass, improvement of animal feed digestibility, biogas and bioethanol production, and various other applications. However, there are only limited reports that describe proteolytic enzymes and esterases in these fungi and their synergistic action with lignocellulolytic enzymes on degradation of complex polymers. Thus, in this minireview, we focus on the importance of these organisms in enzyme technology, their bioprospecting, possibility of integration of their enzyme repertoire, and their prospects for future biotechnological innovation.
[Mh] Termos MeSH primário: Bioprospecção/métodos
Biotecnologia/métodos
Neocallimastigomycota/enzimologia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Anaerobiose
Biocombustíveis
Biomassa
Biotecnologia/tendências
Esterases/metabolismo
Lignina/metabolismo
Neocallimastigomycota/metabolismo
Peptídeo Hidrolases/metabolismo
Polímeros/metabolismo
Madeira/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biofuels); 0 (Polymers); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 3.1.- (Esterases); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170319
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8225-5


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[PMID]:28272809
[Au] Autor:Martorell MM; Ruberto LAM; Fernández PM; Castellanos de Figueroa LI; Mac Cormack WP
[Ad] Endereço:Instituto Antártico Argentino (IAA), CONICET, Buenos Aires, Argentina.
[Ti] Título:Bioprospection of cold-adapted yeasts with biotechnological potential from Antarctica.
[So] Source:J Basic Microbiol;57(6):504-516, 2017 Jun.
[Is] ISSN:1521-4028
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia. Results from enzymes production at low temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which represent potential tools for biotechnological applications. While most the isolates proved to produce 2-4 of the investigated exoenzymes, two of them evidenced the six evaluated enzymatic activities: Pichia caribbica and Guehomyces pullulans, which were characterized as psycrotolerant and psycrophilic, respectively. In addition, P. caribbica could assimilate several n-alkanes and diesel fuel. The enzyme production profile and hydrocarbons assimilation capacity, combined with its high level of biomass production and the extended exponential growth phase make P. caribbica a promising tool for cold environments biotechnological purposes in the field of cold-enzymes production and oil spills bioremediation as well.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Ascomicetos/metabolismo
Basidiomycota/metabolismo
Bioprospecção
Biotecnologia
Temperatura Baixa
Microbiologia do Solo
[Mh] Termos MeSH secundário: Regiões Antárticas
Ascomicetos/enzimologia
Ascomicetos/isolamento & purificação
Basidiomycota/enzimologia
Basidiomycota/isolamento & purificação
Biodegradação Ambiental
Biomassa
DNA Fúngico
Proteínas Fúngicas/genética
Gasolina
Hidrocarbonetos/metabolismo
Filogenia
Pichia/enzimologia
Pichia/crescimento & desenvolvimento
Pichia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (Fungal Proteins); 0 (Gasoline); 0 (Hydrocarbons)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1002/jobm.201700021


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[PMID]:28168318
[Au] Autor:Tanvir R; Javeed A; Bajwa AG
[Ad] Endereço:University Diagnostic Lab (UDL), Department of Microbiology, University of Veterinary and Animal Sciences (UVAS), Lahore, 54000, Punjab, Pakistan. rabia.tanvir@uvas.edu.pk.
[Ti] Título:Endophyte bioprospecting in South Asian medicinal plants: an attractive resource for biopharmaceuticals.
[So] Source:Appl Microbiol Biotechnol;101(5):1831-1844, 2017 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:From the last several years, there has been an increasing interest in plant-associated bacteria commonly referred to as endophytes that reside asymptomatically in the internal plant tissues. This interest peaked since the last two decades due to the recognition that endophytes within medicinal plants have the capability to mimic and produce the bioactive metabolites of the host plant. A number of medicinal plants have been used for centuries by the people of South Asia to cure various diseases and this has led to abundant usage experience. Relating to prior ethanopharmacological experiences, scientists have searched for medicinal plants that could be valued sources for endophytes yielding novel metabolites of pharmaceutical importance. This review is therefore an effort to encompass the most recent efforts in the exploration of medicinal plants of South Asia and their endophytes.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Bioprospecção/métodos
Descoberta de Drogas/métodos
Endófitos/metabolismo
Plantas Medicinais/microbiologia
[Mh] Termos MeSH secundário: Ásia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8115-x


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[PMID]:28107573
[Au] Autor:Nalini MS; Prakash HS
[Ad] Endereço:Department of Studies in Botany, University of Mysore, Mysore, India.
[Ti] Título:Diversity and bioprospecting of actinomycete endophytes from the medicinal plants.
[So] Source:Lett Appl Microbiol;64(4):261-270, 2017 Apr.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The endophytic actinomycetes constitute one of the fascinating group of microorganisms associated with a wide range of plant species. The diversity of actinomycetes in plants and their tissue parts is a matter of debate as no consensus are derived between individual studies. Nevertheless, their diversity correlates with the occurrence in plant species harboured in unique regions of biologically diverse areas called "hot spots." Recent advances in the isolation techniques have facilitated the isolation of rare taxa from these environments. The biosynthetic ability of the endophytic actinomycetes has proven beyond doubt that these organisms have the potential to synthesize an array of compounds with novelty in structure and bioactivity and as a result are preferred in the natural product screening programs. In the years to come, the scientific world may await to discover many more novel actinomycete taxa with metabolic diversity and applications in therapeutics. SIGNIFICANCE AND IMPACT OF THE STUDY: "Endophytes" - the microbes residing in the living tissues of plants are virtually omnipresent. Actinomycete endophytes are diverse in distribution within plant tissues, especially in the roots as they have a close association with the rhizhosphere. An introspection into diversity studies necessitates careful sampling, analysis, and isolation data from the biodiverse and nonbiodiverse regions represented by unique environments. The key to the recovery of novel species and their bioprospection lies in these regions.
[Mh] Termos MeSH primário: Actinobacteria/isolamento & purificação
Plantas Medicinais/microbiologia
[Mh] Termos MeSH secundário: Actinobacteria/genética
Biodiversidade
Produtos Biológicos/isolamento & purificação
Bioprospecção
Endófitos
Geografia
Raízes de Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biological Products)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12718


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[PMID]:27936316
[Au] Autor:Stranska-Zachariasova M; Kurniatanty I; Gbelcova H; Jiru M; Rubert J; Nindhia TG; D'Acunto CW; Sumarsono SH; Tan MI; Hajslova J; Ruml T
[Ad] Endereço:Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague, Czech Republic.
[Ti] Título:Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds.
[So] Source:Chem Biodivers;14(2), 2017 Feb.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e., H O, MeOH/H O (80:20, v/v) and hexane/i-PrOH (50:50, v/v), were used for extraction. Both the biological activity and the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix/breast/pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed. To complete the information about macroalgae composition, organic compounds were characterized by the liquid chromatography coupled with high resolution tandem mass spectrometry. Attention was concentrated mainly on the lipidomic profile characterization. In spite the fact that any significant antiproliferative effect was not observed for cancer cells, both the Turbinaria species were shown to be good protectors against the oxidative stress of the non-cancer cells. Most of the antioxidants were determined in the hexane/i-PrOH extract. As regards the lipids identified, most of them belonged to the triacylglycerols followed by sphingomyelins, diacylglycerols, and polar (lyso)phospholipids. Additionally to fatty acids with 14, 16 and 18 carbons, also those with odd carbon numbers were frequently present.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Produtos Biológicos/química
Bioprospecção/métodos
Proliferação Celular/efeitos dos fármacos
Microalgas/química
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Células Cultivadas
Cromatografia Líquida de Alta Pressão
Seres Humanos
Lipídeos/análise
Lipídeos/química
Metaboloma
Metabolômica
Microalgas/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biological Products); 0 (Lipids); 0 (Protective Agents)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201600192


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[PMID]:27896615
[Au] Autor:Sahoo RK; Kumar M; Sukla LB; Subudhi E
[Ad] Endereço:Centre of Biotechnology, Siksha 'O' Anusandhan University, Kalinga Nagar, Bhubaneswar, Odisha, India.
[Ti] Título:Bioprospecting hot spring metagenome: lipase for the production of biodiesel.
[So] Source:Environ Sci Pollut Res Int;24(4):3802-3809, 2017 Feb.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65 °C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75 °C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate. RK-lip479 was resistant to many organic solvents, especially isopropanol, DMSO, methanol, DMF, ethanol, dichloromethane, acetone, glycerol and ethyl acetate. RK-lip479 also showed activity in the presence of monovalent (Na and K ), divalent (Mg , Mn , Ca , Hg , Cu , Co , Zn and Ag ) and trivalent cations (Fe and Al ). Yield of biodiesel production was in the range of 40-76% using various waste oils with RK-Lip479 under optimized conditions.
[Mh] Termos MeSH primário: Lipase/metabolismo
Metagenoma
[Mh] Termos MeSH secundário: Biocombustíveis
Bioprospecção
Fontes Termais
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-8118-7


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[PMID]:27856231
[Au] Autor:Choudhary J; Singh S; Nain L
[Ad] Endereço:Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
[Ti] Título:Bioprospecting thermotolerant ethanologenic yeasts for simultaneous saccharification and fermentation from diverse environments.
[So] Source:J Biosci Bioeng;123(3):342-346, 2017 Mar.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Lignocellulosic biomass, a promising renewable energy source, can be used for the production of second generation bioethanol. Simultaneous saccharification and fermentation (SSF), the process which alleviates the problem of separate hydrolysis and fermentation (SHF), requires thermotolerant ethanologenic yeast for bioethanol production. Therefore, ten yeast strains isolated from diverse sources, belonging to various genera like Saccharomyces, Candida, Pichia and Wickerhamomyces were evaluated for their thermotolerance, sugar utilization pattern, inhibitor tolerance and ethanol production potential with glucose, xylose and alkali pretreated paddy straw. All the tested strains were found to be thermotolerant, capable of significant growth at 40°C. Candida tropicalis Y6 was capable of utilizing a wide range of sugars as compared with other yeast isolates. Strains of Candida showed better inhibitor tolerance as compared to Saccharomyces and Pichia strains and exhibited only 5.1-18.8% and 4.7-7.9% reduction in growth with furfural and 5-hydroxymethyl furfural, respectively. Saccharomyces cerevisiae JRC6, isolated from distillery waste, produced ethanol with 88.3% and 89.1% theoretical efficiency at 40°C and 42°C, respectively, from glucose. This strain also produced significantly higher amount of ethanol (3.8 g/L) with better fermentation efficiency (87.9%) from alkali pretreated paddy straw at 40°C, as compared with the other yeast strains. Therefore, S. cerevisiae JRC6, based on its ability to ferment sugars at a higher temperature, can be a promising candidate for production of ethanol from lignocellulosic biomass via SSF process.
[Mh] Termos MeSH primário: Bioprospecção
Etanol/metabolismo
Fermentação
Temperatura Ambiente
Leveduras/isolamento & purificação
Leveduras/metabolismo
[Mh] Termos MeSH secundário: Álcalis
Biomassa
Candida/efeitos dos fármacos
Candida/metabolismo
Etanol/isolamento & purificação
Etanol/provisão & distribuição
Fermentação/efeitos dos fármacos
Furaldeído/análogos & derivados
Furaldeído/metabolismo
Furaldeído/farmacologia
Glucose/metabolismo
Glucose/farmacologia
Hidrólise/efeitos dos fármacos
Lignina/metabolismo
Pichia/efeitos dos fármacos
Pichia/metabolismo
Saccharomyces cerevisiae/efeitos dos fármacos
Saccharomyces cerevisiae/metabolismo
Saccharomycetales/efeitos dos fármacos
Saccharomycetales/metabolismo
Xilose/metabolismo
Xilose/farmacologia
Leveduras/classificação
Leveduras/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 11132-73-3 (lignocellulose); 3K9958V90M (Ethanol); 70ETD81LF0 (5-hydroxymethylfurfural); 9005-53-2 (Lignin); A1TA934AKO (Xylose); DJ1HGI319P (Furaldehyde); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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Vilegas, Wagner
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[PMID]:27644133
[Au] Autor:Tangerina MM; Correa H; Haltli B; Vilegas W; Kerr RG
[Ad] Endereço:Biosciences Institute, São Paulo State University - UNESP, Coastal Campus, Praça Infante Dom Henrique s/n, São Vicente, SP, P.O. Box 73601, 11380-972, Brazil.
[Ti] Título:Bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored Ubatuba region of Brazil.
[So] Source:Arch Microbiol;199(1):155-169, 2017 Jan.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Shrimp fisheries along the Brazilian coast have significant environmental impact due to high by-catch rates (21 kg per kilogram of shrimp). Typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. Therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate species, the snail Olivancillaria urceus and the sea star Luidia senegalensis. Sediment from the collection area was also investigated. Utilizing multiple isolation approaches, 134 isolates were obtained from the invertebrates and sediment. Small-subunit rRNA (16S) gene sequencing revealed that the isolates belonged to Proteobacteria, Firmicutes and Actinobacteria phyla and were distributed among 28 genera. Several genera known for their capacity to produce bioactive natural products (Micromonospora, Streptomyces, Serinicoccus and Verrucosispora) were retrieved from the invertebrate samples. To query the bacterial isolates for their ability to produce bioactive metabolites, all strains were fermented and fermentation extracts profiled by UP LC-HRMS and tested for antimicrobial activity. Four strains exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus warneri.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Sedimentos Geológicos/microbiologia
Invertebrados/microbiologia
Caramujos/microbiologia
Estrelas-do-Mar/microbiologia
[Mh] Termos MeSH secundário: Animais
Bactérias/química
Bactérias/classificação
Bactérias/genética
Bioprospecção
Brasil
Filogenia
Resíduos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Products)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-016-1290-9


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[PMID]:27614749
[Au] Autor:Sarmiento-Vizcaíno A; González V; Braña AF; Palacios JJ; Otero L; Fernández J; Molina A; Kulik A; Vázquez F; Acuña JL; García LA; Blanco G
[Ad] Endereço:Departamento de Biología Funcional, Área de Microbiología, e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, 33006, Oviedo, Spain.
[Ti] Título:Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea.
[So] Source:Microb Ecol;73(2):338-352, 2017 Feb.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest.
[Mh] Termos MeSH primário: Actinobacteria/química
Actinobacteria/classificação
Actinobacteria/isolamento & purificação
Antozoários/microbiologia
Produtos Biológicos/farmacologia
Filogenia
[Mh] Termos MeSH secundário: Actinobacteria/genética
Animais
Antibacterianos/farmacologia
Antineoplásicos/farmacologia
Bactérias/efeitos dos fármacos
Sequência de Bases
Biodiversidade
Produtos Biológicos/química
Produtos Biológicos/isolamento & purificação
Bioprospecção
Linhagem Celular Tumoral/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Classificação
Recifes de Corais
DNA Bacteriano
Ecossistema
Cromatografia Gasosa-Espectrometria de Massas
Genes Bacterianos
Invertebrados/microbiologia
Biologia Marinha
Extratos Vegetais
RNA Ribossômico 16S/genética
Água do Mar
Metabolismo Secundário
Espanha
Streptomyces/classificação
Streptomyces/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 0 (Biological Products); 0 (DNA, Bacterial); 0 (Plant Extracts); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160912
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0845-2


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[PMID]:27702563
[Au] Autor:Orozco RA; Molnár I; Bode H; Stock SP
[Ad] Endereço:Department of Entomology, University of Arizona, 1140 E. South Campus Dr., Tucson, AZ 85721-0036, United States.
[Ti] Título:Bioprospecting for secondary metabolites in the entomopathogenic bacterium Photorhabdus luminescens subsp. sonorensis.
[So] Source:J Invertebr Pathol;141:45-52, 2016 Nov.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Crude extracts of in vitro and in vivo cultures of two strains of Photorhabdus l. sonorensis (Enterobacteriaceae) were analyzed by TLC, HPLC-UV and LC-MS. Nine unique compounds with mass/charge ratios (m/z) ranging from 331.3 to 713.5 were found in MS analyses. Bioactivity of extracts was assessed on a selection of plant pathogens/pests and non-target species. Caborca strain extracts showed the highest activity against Helicoverpa zea (Lepidoptera: Noctuidae) neonates at all concentrations tested. Mortality ranged from 11% (at 10µg/ml) to 37% (at 40µg/ml). Strain CH35 extracts showed the highest nematicidal activity on Meloidogyne incognita (Tylenchida: Meloidogynidae) at 40µg/ml. Low to no nematicidal activity was observed against the non-target species Steinernema carpocapsae (Rhabditida: Steinernematidae) and Caenorhabditis elegans (Rhabditida: Rhabditidae). Caborca extracts exhibited a strong antibiotic effect on Pseudomonas syringae (Pseudomonadales: Pseudomonadacedae) at 40µg/ml, while both Caborca and CH35 extracts inhibited the growth of Bacillus subitillis (Bacillales: Bacillaceae) at 40µg/ml. All extracts strongly inhibited the growth of the fungus Fusarium oxysporum (Hypocreales: Nectriceae) but not that of Alternaria alternata (Pleosporales: Pleosporaceae). Contrastingly, a moderate to high inhibitory effect was denoted on the non-target biocontrol fungus Beauveria bassiana (Hypocreales: Clavivipitaceae).
[Mh] Termos MeSH primário: Bioprospecção/métodos
Misturas Complexas/farmacologia
Controle Biológico de Vetores/métodos
Photorhabdus/química
Photorhabdus/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Cromatografia em Camada Delgada
Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE



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