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  1 / 11422 MEDLINE  
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[PMID]:29385196
[Au] Autor:Milanese C; Cavedon V; Sandri M; Tam E; Piscitelli F; Boschi F; Zancanaro C
[Ad] Endereço:Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
[Ti] Título:Metabolic effect of bodyweight whole-body vibration in a 20-min exercise session: A crossover study using verified vibration stimulus.
[So] Source:PLoS One;13(1):e0192046, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability of whole body vibration (WBV) to increase energy expenditure (EE) has been investigated to some extent in the past using short-term single exercises or sets of single exercises. However, the current practice in WBV training for fitness is based on the execution of multiple exercises during a WBV training session for a period of at least 20 min; nevertheless, very limited and inconsistent data are available on EE during long term WBV training session. This crossover study was designed to demonstrate, in an adequately powered sample of participants, the ability of WBV to increase the metabolic cost of exercise vs. no vibration over the time span of a typical WBV session for fitness (20 min). Twenty-two physically active young males exercised on a vibration platform (three identical sets of six different exercises) using an accelerometer-verified vibration stimulus in both the WBV and no vibration condition. Oxygen consumption was measured with indirect calorimetry and expressed as area under the curve (O2(AUC)). Results showed that, in the overall 20-min training session, WBV increased both the O2(AUC) and the estimated EE vs. no vibration by about 22% and 20%, respectively (P<0.001 for both, partial eta squared [η2] ≥0.35) as well as the metabolic equivalent of task (+5.5%, P = 0.043; η2 = 0.02) and the rate of perceived exertion (+13%, P<0.001; Å‹2 = 0.16). Results demonstrated that vibration is able to significantly increase the metabolic cost of exercise in a 20-min WBV training session.
[Mh] Termos MeSH primário: Peso Corporal
Exercício
Vibração
[Mh] Termos MeSH secundário: Antropometria
Calorimetria
Estudos Cross-Over
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192046


  2 / 11422 MEDLINE  
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[PMID]:28985538
[Au] Autor:Chillè D; Foti C; Giuffrè O
[Ad] Endereço:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, 98166 Messina, Italy.
[Ti] Título:Thermodynamic parameters for the protonation and the interaction of arsenate with Mg , Ca and Sr : Application to natural waters.
[So] Source:Chemosphere;190:72-79, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thermodynamic parameters for the protonation of AsO and for the interaction with Mg , Ca and Sr were reported, comprehensive also of their dependence on ionic strength, considering the 0.1 ≤ I ≤ 1 M range and using NaCl as background salt. The same speciation models were obtained for Mg , Ca and Sr systems, with the formation of three different species: ML, MLH and MLH (L = AsO ). Mono- and di-protonated species were very weak, with formation constant values (log K) ranging from 1.45 to 3.23. In order to have a complete picture of thermodynamic properties of the systems under study and to fill the shortage of thermodynamic data on arsenate complex systems, the ligand protonation and metal complex enthalpies were also determined by calorimetric titrations, at t = 25 °C and in NaCl at I = 0.7 M (for H -AsO species also at I = 0.1 M). On the light of the proposed speciation models, examples of As(V) distribution in some natural waters are reported.
[Mh] Termos MeSH primário: Arseniatos/química
Concentração Osmolar
Termodinâmica
Água/química
[Mh] Termos MeSH secundário: Cálcio/química
Calorimetria
Ligantes
Magnésio/química
Cloreto de Sódio/química
Estrôncio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Ligands); 059QF0KO0R (Water); 451W47IQ8X (Sodium Chloride); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium); YZS2RPE8LE (Strontium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


  3 / 11422 MEDLINE  
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[PMID]:29274339
[Au] Autor:Alniss H; Zamiri B; Khalaj M; Pearson CE; Macgregor RB
[Ad] Endereço:College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates.
[Ti] Título:Thermodynamic and spectroscopic investigations of TMPyP4 association with guanine- and cytosine-rich DNA and RNA repeats of C9orf72.
[So] Source:Biochem Biophys Res Commun;495(4):2410-2417, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An expansion of the hexanucleotide repeat (GGGGCC)n·(GGCCCC)n in the C9orf72 promoter has been shown to be the cause of Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The C9orf72 repeat can form four-stranded structures; the cationic porphyrin (TMPyP4) binds and distorts these structures. METHODS: Isothermal titration calorimetry (ITC), and circular dichroism (CD) were used to study the binding of TMPyP4 to the C-rich and G-rich DNA and RNA oligos containing the hexanucleotide repeat at pH 7.5 and 0.1 M K . RESULTS: The CD spectra of G-rich DNA and RNA TMPyP4 complexes showed features of antiparallel and parallel G-quadruplexes, respectively. The shoulder at 260 nm in the CD spectrum becomes more intense upon formation of complexes between TMPyP4 and the C-rich DNA. The peak at 290 nm becomes more intense in the c-rich RNA molecules, suggesting induction of an i-motif structure. The ITC data showed that TMPyP4 binds at two independent sites for all DNA and RNA molecules. CONCLUSIONS: For DNA, the data are consistent with TMPyP4 stacking on the terminal tetrads and intercalation. For RNA, the thermodynamics of the two binding modes are consistent with groove binding and intercalation. In both cases, intercalation is the weaker binding mode. These findings are considered with respect to the structural differences of the folded DNA and RNA molecules and the energetics of the processes that drive site-specific recognition by TMPyP4; these data will be helpful in efforts to optimize the specificity and affinity of the binding of porphyrin-like molecules.
[Mh] Termos MeSH primário: Proteína C9orf72/química
Proteína C9orf72/genética
Citosina/química
DNA/química
Guanina/química
RNA/química
Sequências Repetitivas de Ácido Nucleico
[Mh] Termos MeSH secundário: Composição de Bases
Sítios de Ligação
Calorimetria
Dicroísmo Circular
DNA/genética
Ligação Proteica
RNA/genética
Relação Estrutura-Atividade
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (C9orf72 Protein); 5Z93L87A1R (Guanine); 63231-63-0 (RNA); 8J337D1HZY (Cytosine); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


  4 / 11422 MEDLINE  
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[PMID]:29381744
[Au] Autor:Biniaminov N; Bandt S; Roth A; Haertel S; Neumann R; Bub A
[Ad] Endereço:Institute of Sports and Sports Science, Karlsruhe Institute of Technology, Karlsruhe, Germany.
[Ti] Título:Irisin, physical activity and fitness status in healthy humans: No association under resting conditions in a cross-sectional study.
[So] Source:PLoS One;13(1):e0189254, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Regular physical activity and physical fitness are closely related to a positive health status in humans. In this context, the muscle becomes more important due to its function as an endocrine organ. Muscle tissue secretes "myokines" in response to physical activity and it is speculated that these myokines are involved in physical activity induced positive health effects. Recently, the newly discovered myokine Irisin thought to be secreted by the muscle in response to physical activity and might be related to the health inducing effect by inducing browning of white adipose tissue. Speculating that myokines at least partly mediate exercise related health effects one would assume that regular physical activity and physical fitness are associated with resting Irisin concentrations in healthy humans. To investigate the association between resting Irisin concentration and either short-term physical activity, habitual physical activity, or physical fitness, data of 300 healthy participants from the cross-sectional KarMeN-study were analyzed. By applying different activity measurements we determined short-term and habitual physical activity, as well as physical fitness. Fasting serum samples were collected to determine resting Irisin concentrations by Enzyme-linked Immunosorbent Assay. Multivariate linear regression analysis served to investigate associations of the individual physical activity parameters with Irisin concentrations. Therefore, lean body mass and total fat mass (both determined by dual-energy X-ray absorptiometry) as well as age and parameters of glucose metabolism were included as confounders in multivariate linear regression analysis. Results showed that Irisin serum concentrations were not related to measures of physical activity and physical fitness in healthy humans under resting conditions, irrespective of the applied methods. Therefore we assume that if physical activity related effects are partly induced by myokines, permanently increased Irisin serum concentration may not be necessary to induce health-related exercise effects.
[Mh] Termos MeSH primário: Fibronectinas/metabolismo
Aptidão Física
Descanso
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Calorimetria
Estudos Transversais
Feminino
Seres Humanos
Masculino
Meia-Idade
Músculo Esquelético/secreção
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (FNDC5 protein, human); 0 (Fibronectins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189254


  5 / 11422 MEDLINE  
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[PMID]:29351348
[Au] Autor:Jasinski M; Kulik M; Wojciechowska M; Stolarski R; Trylska J
[Ad] Endereço:Centre of New Technologies, University of Warsaw, Warsaw, Poland.
[Ti] Título:Interactions of 2'-O-methyl oligoribonucleotides with the RNA models of the 30S subunit A-site.
[So] Source:PLoS One;13(1):e0191138, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Synthetic oligonucleotides targeting functional regions of the prokaryotic rRNA could be promising antimicrobial agents. Indeed, such oligonucleotides were proven to inhibit bacterial growth. 2'-O-methylated (2'-O-Me) oligoribonucleotides with a sequence complementary to the decoding site in 16S rRNA were reported as inhibitors of bacterial translation. However, the binding mode and structures of the formed complexes, as well as the level of selectivity of the oligonucleotides between the prokaryotic and eukaryotic target, were not determined. We have analyzed three 2'-O-Me oligoribonucleotides designed to hybridize with the models of the prokaryotic rRNA containing two neighboring aminoglycoside binding pockets. One pocket is the paromomycin/kanamycin binding site corresponding to the decoding site in the small ribosomal subunit and the other one is the close-by hygromycin B binding site whose dynamics has not been previously reported. Molecular dynamics (MD) simulations, as well as isothermal titration calorimetry, gel electrophoresis and spectroscopic studies have shown that the eukaryotic rRNA model is less conformationally stable (in terms of hydrogen bonds and stacking interactions) than the corresponding prokaryotic one. In MD simulations of the eukaryotic construct, the nucleotide U1498, which plays an important role in correct positioning of mRNA during translation, is flexible and spontaneously flips out into the solvent. In solution studies, the 2'-O-Me oligoribonucleotides did not interact with the double stranded rRNA models but all formed stable complexes with the single-stranded prokaryotic target. 2'-O-Me oligoribonucleotides with one and two mismatches bound less tightly to the eukaryotic target. This shows that at least three mismatches between the 2'-O-Me oligoribonucleotide and eukaryotic rRNA are required to ensure target selectivity. The results also suggest that, in the ribosome environment, the strand invasion is the preferred binding mode of 2'-O-Me oligoribonucleotides targeting the aminoglycoside binding sites in 16S rRNA.
[Mh] Termos MeSH primário: Modelos Moleculares
Oligonucleotídeos/química
RNA Ribossômico 16S/química
[Mh] Termos MeSH secundário: Calorimetria
Eletroforese em Gel de Poliacrilamida
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191138


  6 / 11422 MEDLINE  
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[PMID]:29293679
[Au] Autor:Bauters D; Bedossa P; Lijnen HR; Hemmeryckx B
[Ad] Endereço:Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences, University of Leuven, Leuven, Belgium.
[Ti] Título:Functional role of ADAMTS5 in adiposity and metabolic health.
[So] Source:PLoS One;13(1):e0190595, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies with gene-deficient mice (ADAMTS5-P) revealed that ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin type 1 motifs, member 5) plays a functional role in adiposity and metabolic health. To confirm these observations, we have performed similar studies with an independently generated strain of ADAMTS5 deficient mice (ADAMTS5-J). Upon cold exposure as well as after high-fat diet feeding (diet-induced obesity or DIO model), these knockout (KO) mice developed less subcutaneous and gonadal white adipose tissue (WAT) as compared to their wild-type (WT) littermates (reduction was more pronounced in ADAMTS5-P mice). Enhanced browning of WAT, as monitored by expression of UCP-1 was seen in the ADAMTS5-J KO mice upon cold exposure but not in the DIO model (seen in both conditions with the ADAMTS5-P mice). Brown adipose tissue (BAT) mass was not different between KO and WT ADAMTS5-J mice, either upon cold exposure or in the DIO model (in contrast to the enhanced BAT mass with the ADAMTS5-P mice). Energy expenditure and thermogenesis were not significantly different between KO and WT ADAMTS5-J mice (in contrast to somewhat enhanced levels in ADAMTS5-P mice). Insulin sensitivity was improved in the ADAMTS5-J KO mice, and they were protected against non-alcoholic steatohepatitis in the DIO model (as the ADAMTS5-P mice). These data are thus similar for both strains of KO mice, confirming specificity of the phenotype, but some quantitative and qualitative differences are also observed.
[Mh] Termos MeSH primário: Proteína ADAMTS5/fisiologia
Adiposidade/fisiologia
[Mh] Termos MeSH secundário: Proteína ADAMTS5/genética
Tecido Adiposo Marrom/metabolismo
Animais
Western Blotting
Calorimetria
Temperatura Baixa
Dieta Hiperlipídica
Metabolismo Energético
Regulação da Expressão Gênica
Camundongos
Camundongos Knockout
Termogênese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.4.24.- (ADAMTS5 Protein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190595


  7 / 11422 MEDLINE  
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[PMID]:29298351
[Au] Autor:Gerszon J; Serafin E; Buczkowski A; Michlewska S; Bielnicki JA; Rodacka A
[Ad] Endereço:Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
[Ti] Título:Functional consequences of piceatannol binding to glyceraldehyde-3-phosphate dehydrogenase.
[So] Source:PLoS One;13(1):e0190656, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key redox-sensitive proteins whose activity is largely affected by oxidative modifications at its highly reactive cysteine residue in the enzyme's active site (Cys149). Prolonged exposure to oxidative stress may cause, inter alia, the formation of intermolecular disulfide bonds leading to accumulation of GAPDH aggregates and ultimately to cell death. Recently these anomalies have been linked with the pathogenesis of Alzheimer's disease. Novel evidences indicate that low molecular compounds may be effective inhibitors potentially preventing the GAPDH translocation to the nucleus, and inhibiting or slowing down its aggregation and oligomerization. Therefore, we decided to establish the ability of naturally occurring compound, piceatannol, to interact with GAPDH and to reveal its effect on functional properties and selected parameters of the dehydrogenase structure. The obtained data revealed that piceatannol binds to GAPDH. The ITC analysis indicated that one molecule of the tetrameric enzyme may bind up to 8 molecules of polyphenol (7.3 ± 0.9). Potential binding sites of piceatannol to the GAPDH molecule were analyzed using the Ligand Fit algorithm. Conducted analysis detected 11 ligand binding positions. We indicated that piceatannol decreases GAPDH activity. Detailed analysis allowed us to presume that this effect is due to piceatannol ability to assemble a covalent binding with nucleophilic cysteine residue (Cys149) which is directly involved in the catalytic reaction. Consequently, our studies strongly indicate that piceatannol would be an exceptional inhibitor thanks to its ability to break the aforementioned pathologic disulfide linkage, and therefore to inhibit GAPDH aggregation. We demonstrated that by binding with GAPDH piceatannol blocks cysteine residue and counteracts its oxidative modifications, that induce oligomerization and GAPDH aggregation.
[Mh] Termos MeSH primário: Gliceraldeído-3-Fosfato Desidrogenases/metabolismo
Estilbenos/metabolismo
[Mh] Termos MeSH secundário: Calorimetria
Domínio Catalítico
Dicroísmo Circular
Gliceraldeído-3-Fosfato Desidrogenases/química
Peróxido de Hidrogênio/metabolismo
Ligantes
Microscopia Eletrônica de Transmissão
Modelos Moleculares
Ligação Proteica
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ligands); 0 (Stilbenes); 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene); BBX060AN9V (Hydrogen Peroxide); EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190656


  8 / 11422 MEDLINE  
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[PMID]:29178995
[Au] Autor:Das S; Chatterjee S; Pramanik S; Devi PS; Kumar GS
[Ad] Endereço:Sensor and Actuator Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India.
[Ti] Título:A new insight into the interaction of ZnO with calf thymus DNA through surface defects.
[So] Source:J Photochem Photobiol B;178:339-347, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Experimental evidences on the binding interaction of ZnO and Calf Thymus (CT) DNA using several biophysical techniques are the centre of interest of the present study. The interaction of ZnO with CT DNA has been investigated in detail by absorption spectral study, fluorescence titration, Raman analysis, zeta potential measurement, viscometric experiment along with thermal melting study and microscopic analysis. Steady-state fluorescence study revealed the quenching (48%) of the surface defect related peak intensity of ZnO on interaction with DNA. The optimized concentration of ZnO and DNA to obtain this level of quenching has been found to be 0.049mM and 1.027µM, respectively. Additional fluorescence study with 8-hydroxy-5-quinoline (HQ) as a fluorescence probe for Zn ruled out the dissolution effect of ZnO under the experimental conditions. DNA conjugation on the surface of ZnO was also supported by Raman study. The quantitative variation in conductivity as well as electrophoretic mobility indicated significant interaction of ZnO with the DNA molecule. Circular dichroism (CD) and viscometry titrations provided clear evidence in support of the conformational retention of the DNA on interaction with ZnO. The binding interaction was found to be predominantly entropy driven in nature. The bio-physical studies presented in this paper exploring ZnO-CT DNA interaction could add a new horizon to understand the interaction between metal oxide and DNA.
[Mh] Termos MeSH primário: DNA/química
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Animais
Calorimetria
Bovinos
Dicroísmo Circular
Ensaio de Desvio de Mobilidade Eletroforética
Microscopia Eletrônica de Transmissão
Oxiquinolina/química
Espectrometria de Fluorescência
Análise Espectral Raman
Propriedades de Superfície
Termodinâmica
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5UTX5635HP (Oxyquinoline); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  9 / 11422 MEDLINE  
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[PMID]:27775109
[Au] Autor:Zhang XR; Zhang Y; Chen FT; Li Y; Zhang SS
[Ad] Endereço:Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
[Ti] Título:Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles.
[So] Source:Chem Commun (Camb);52(90):13261-13264, 2016 Nov 03.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs). As a proof-of-principle, novel applications of this method as a versatile biosensor for single-nucleotide polymorphisms (SNPs) and DNA methyltransferase (MTase) were presented.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Calorimetria/métodos
Cobre/química
Metilases de Modificação do DNA/metabolismo
Ouro/química
Nanopartículas Metálicas/química
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Química Click
Troca Iônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7440-57-5 (Gold); 789U1901C5 (Copper); EC 2.1.1.- (DNA Modification Methylases); KL4YU612X7 (cupric sulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE


  10 / 11422 MEDLINE  
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[PMID]:29337670
[Au] Autor:Hanif M; Khan HU; Afzal S; Mahmood A; Maheen S; Afzal K; Iqbal N; Andleeb M; Abbas N
[Ad] Endereço:1Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
[Ti] Título:Sustained release biodegradable solid lipid microparticles: Formulation, evaluation and statistical optimization by response surface methodology.
[So] Source:Acta Pharm;67(4):441-461, 2017 Dec 20.
[Is] ISSN:1846-9558
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:For preparing nebivolol loaded solid lipid microparticles (SLMs) by the solvent evaporation microencapsulation process from carnauba wax and glyceryl monostearate, central composite design was used to study the impact of independent variables on yield (Y1), entrapment efficiency (Y2) and drug release (Y3). SLMs having a 10-40 µm size range, with good rheological behavior and spherical smooth surfaces, were produced. Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray diffractometry pointed to compatibility between formulation components and the zeta-potential study confirmed better stability due to the presence of negative charge (-20 to -40 mV). The obtained outcomes for Y1 (29-86 %), Y2 (45-83 %) and Y3 (49-86 %) were analyzed by polynomial equations and the suggested quadratic model were validated. Nebivolol release from SLMs at pH 1.2 and 6.8 was significantly (p < 0.05) affected by lipid concentration. The release mechanism followed Higuchi and zero order models, while n > 0.85 value (Korsmeyer- Peppas) suggested slow erosion along with diffusion. The optimized SLMs have the potential to improve nebivolol oral bioavailability.
[Mh] Termos MeSH primário: Preparações de Ação Retardada/química
Composição de Medicamentos/métodos
Glicerídeos/química
Ceras/química
[Mh] Termos MeSH secundário: Calorimetria/métodos
Portadores de Fármacos/química
Nebivolol/farmacocinética
Tamanho da Partícula
Espectroscopia de Infravermelho com Transformada de Fourier
Propriedades de Superfície
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Delayed-Action Preparations); 0 (Drug Carriers); 0 (Glycerides); 0 (Waxes); 030Y90569U (Nebivolol); 230OU9XXE4 (glyceryl monostearate); R12CBM0EIZ (carnauba wax)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE



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