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[PMID]:28748727
[Au] Autor:Yan S; Pan S; Ji J
[Ad] Endereço:a School of Petrochemical Engineering , Changzhou University , Changzhou , PR China.
[Ti] Título:Silk fabric dyed with extract of sophora flower bud.
[So] Source:Nat Prod Res;32(3):308-315, 2018 Feb.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study analysed the use of sophora flower bud extract for dyeing and the resulting colour character and fastness of dyed silk fabric. The pigment composition on the silk fabric and recycling of this extract were also studied. The results indicated that the dyed silk fabric possessed good washing, rubbing and perspiration fastness, and the pigment composition on the silk fabric was mainly rutin and quercetin. The average recovery rate of the dye was 55.00%. These results demonstrate that the sophora flower bud extract is an effective natural dye.
[Mh] Termos MeSH primário: Corantes/química
Extratos Vegetais/química
Seda/química
Sophora/química
Têxteis
[Mh] Termos MeSH secundário: Precipitação Química
Cromatografia Líquida de Alta Pressão
Corantes/análise
Flores/química
Espectroscopia de Ressonância Magnética
Extratos Vegetais/análise
Quercetina/análise
Rutina/análise
Espectroscopia de Infravermelho com Transformada de Fourier
Têxteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Plant Extracts); 0 (Silk); 5G06TVY3R7 (Rutin); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1359170


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[PMID]:29426296
[Au] Autor:Iu LPL; Fan MCY; Lam WC; Wong IYH
[Ad] Endereço:Department of Ophthalmology, The University of Hong Kong, Grantham Hospital, 125 Wong Chuk Hang Road, Aberdeen, Hong Kong, Hong Kong. lawipl@hku.hk.
[Ti] Título:Repeated intraocular crystallization of ganciclovir in one eye after bilateral intravitreal injections: a case report.
[So] Source:BMC Ophthalmol;18(1):36, 2018 Feb 09.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cytomegalovirus (CMV) retinitis is an opportunistic infection that primarily affects immunocompromised individuals. Intravitreal ganciclovir injection monotherapy or in combination with systemic anti-CMV therapy are effective treatments for CMV retinitis. Crystallization of ganciclovir after intravitreal injection is extremely rare. Only two cases had been reported in literature. Crystallization in only one eye after bilateral injections had not been reported before. We hereby report a case of intraocular ganciclovir crystallization in one eye after bilateral intravitreal injections, and repeated crystallization in the same eye after repeated injections. CASE PRESENTATION: A 79-year-old patient had bilateral cytomegalovirus retinitis and received bilateral intravitreal ganciclovir injections of 2.5 mg in 0.05 ml sterile water. Fundus examination after injection showed formation of needle-shaped, golden-yellow crystals in the vitreous of right eye but not in left eye. The crystals dissolved spontaneously. Repeated bilateral intravitreal ganciclovir injections 4 days later resulted in repeated crystallization of ganciclovir in right eye but not in left eye. The crystals dissolved spontaneously and completely after 5 minutes. Visual acuity remained unchanged and intraocular pressure was normal. CONCLUSIONS: Intraocular ganciclovir crystallization could occur after intravitreal injections. It is important to perform fundus examination after injection. The crystals may dissolve rapidly and vitrectomy may not be necessary. Our case suggested intraocular ganciclovir crystallization is an idiosyncratic phenomenon, subjects to distinctive intraocular environment which could be different between two eyes of the same patient. The susceptible intraocular environment could be persistent leading to repeated crystallization.
[Mh] Termos MeSH primário: Antivirais/química
Precipitação Química
Retinite por Citomegalovirus/tratamento farmacológico
Ganciclovir/química
Corpo Vítreo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Idoso
Antivirais/uso terapêutico
Cristalização
Retinite por Citomegalovirus/diagnóstico
Evolução Fatal
Ganciclovir/uso terapêutico
Seres Humanos
Injeções Intravítreas
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); P9G3CKZ4P5 (Ganciclovir)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180211
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0703-8


  3 / 16506 MEDLINE  
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[PMID]:29301069
[Au] Autor:Li Q; Zhao Z
[Ad] Endereço:College of Light Industry and Food Engineering, Guangxi University , Nanning, Guangxi 530004, People's Republic of China.
[Ti] Título:Characterization of the Structural and Colloidal Properties of α-Lactalbumin/Chitosan Complexes as a Function of Heating.
[So] Source:J Agric Food Chem;66(4):972-978, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This research investigated the interaction between α-lactalbumin (α-la) and chitosan at different temperatures. Chitosan was added to α-la solution (5 g L ) to achieve different α-la/chitosan ratios (8:1, 5:1, and 2:1), which were then subjected to different heating temperatures (20, 70, and 90 °C). The results indicated that a low amount of chitosan (8:1) precipitated α-la molecules. Increasing chitosan to a ratio of 5:1 resulted in exposure of the internal structure of α-la, and those formed complexes had high turbidity and average size, which were decreased by an increasing temperature. A further increase of chitosan to a ratio of 2:1 protected the internal structure of α-la molecules. All samples exhibited a similar adsorption behavior at the air/water interface, but the presence of chitosan significantly increased film elasticity. The produced complexes can be regarded as functional ingredients, which can be used as an emulsifying agent and a delivery material to control the release of bioactive compounds.
[Mh] Termos MeSH primário: Quitosana/química
Coloides/química
Temperatura Alta
Lactalbumina/química
[Mh] Termos MeSH secundário: Adsorção
Precipitação Química
Difusão Dinâmica da Luz
Elasticidade
Indústria Alimentícia
Nefelometria e Turbidimetria
Soluções
Espectrometria de Fluorescência
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Colloids); 0 (Solutions); 9012-76-4 (Chitosan); 9013-90-5 (Lactalbumin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04628


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[PMID]:27773244
[Au] Autor:Thokchom B; Qiu P; Cui M; Park B; Pandit AB; Khim J
[Ad] Endereço:School of Civil, Environmental and Architectural Engineering, Korea University, 5-ga, Anam-dong, Seongbuk-gu, Seoul 136-701, South Korea.
[Ti] Título:Magnetic Pd@Fe O composite nanostructure as recoverable catalyst for sonoelectrohybrid degradation of Ibuprofen.
[So] Source:Ultrason Sonochem;34:262-272, 2017 01.
[Is] ISSN:1873-2828
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present research, the degradation of an emerging pharmaceutical micro-pollutant, Ibuprofen (IBP) by using Pd@Fe O and a hybrid sono-electrolytical (US/EC) treatment system has been demonstrated for the first time. The magnetically separable nanocomposite, Pd@Fe O catalyst was synthesized following co-precipitation method to enhance the efficiency of US/EC system. The synthesized catalyst showed a strong reusable property even after applying for five times and in all the five cases, 100% degradation of IBP was maintained. It not only enhanced the IBP degradation rate, but also reduced the energy consumption of the system by ∼35%. Its strong magnetization value of 64.27emug made it easily separable. Hence, a comprehensive knowledge on the application of combined energy based US/EC system and magnetically separable multifunctional catalysts for degradation of intractable pollutants like Ibuprofen was achieved, assuring that US/EC can be an effective option for IBP treatment.
[Mh] Termos MeSH primário: Poluentes Ambientais/química
Ibuprofeno/química
Nanopartículas de Magnetita/química
Paládio/química
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Adsorção
Catálise
Precipitação Química
Eletroquímica
Concentração de Íons de Hidrogênio
Ferro/química
Reciclagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Magnetite Nanoparticles); 5TWQ1V240M (Palladium); E1UOL152H7 (Iron); WK2XYI10QM (Ibuprofen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  5 / 16506 MEDLINE  
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[PMID]:28986240
[Au] Autor:Acero-Navarro KE; Jiménez-Ramírez M; Villalobos MA; Vargas-Martínez R; Perales-Vela HV; Velasco-García R
[Ad] Endereço:Laboratorio de Osmorregulación, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, C. P. 54090, Mexico.
[Ti] Título:Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa.
[So] Source:Protein Expr Purif;142:53-61, 2018 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes. P. aeruginosa G6PDH is also a key enzyme in the metabolism of various carbon sources, such as glucose, glycerol, fructose, and mannitol. Understanding the kinetic characteristics and mechanisms that control the activity of this enzyme is crucial for future studies in this context. However, one of the impediments to achieving this goal is the limited amount of protein obtained when current purification protocols are implemented, a factor curtailing its biochemical characterization. In this study, we report a fast, efficient and reproducible procedure for the purification of P. aeruginosa G6PDH that can be implemented in a short period (2 days). In order to establish this protocol, the zwf gene, which encodes for this enzyme, was cloned and overexpressed in Escherichia coli cells. In contrast to other procedures, our method is based on protein precipitation with CaCl and further purification by ion exchange chromatography. Using this protocol, we were able to obtain 31 mg/L of pure protein that manifested specific activity of 145.7 U/mg. The recombinant enzyme obtained in this study manifested similar physicochemical and kinetic properties to those reported in previous works for this molecule. The large quantities of active enzyme obtained using this procedure will facilitate its structural characterization and identify differences between P. aeruginosa- and human G6PDH, thus contributing to the search for selective inhibitors against the bacterial enzyme.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Clonagem Molecular/métodos
Glucose/metabolismo
Glucosefosfato Desidrogenase/genética
NADP/biossíntese
Pseudomonas aeruginosa/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Cloreto de Cálcio/química
Precipitação Química
Cromatografia por Troca Iônica
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Engenharia Genética
Glucosefosfato Desidrogenase/isolamento & purificação
Glucosefosfato Desidrogenase/metabolismo
Plasmídeos/química
Plasmídeos/metabolismo
Pseudomonas aeruginosa/enzimologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); 53-59-8 (NADP); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); IY9XDZ35W2 (Glucose); M4I0D6VV5M (Calcium Chloride)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171008
[St] Status:MEDLINE


  6 / 16506 MEDLINE  
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[PMID]:29023592
[Au] Autor:Niu Z; Pang RTK; Liu W; Li Q; Cheng R; Yeung WSB
[Ad] Endereço:Department of Obstetrics and Gynecology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
[Ti] Título:Polymer-based precipitation preserves biological activities of extracellular vesicles from an endometrial cell line.
[So] Source:PLoS One;12(10):e0186534, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extracellular vesicles (EVs) are membrane-bound vesicles released by cells and act as media for transfer of proteins, small RNAs and mRNAs to distant sites. They can be isolated by different methods. However, the biological activities of the purified EVs have seldom been studied. In this study, we compared the use of ultracentrifugation (UC), ultra-filtration (UF), polymer-based precipitation (PBP), and PBP with size-based purification (PBP+SP) for isolation of EVs from human endometrial cells and mouse uterine luminal fluid (ULF). Electron microscopy revealed that the diameters of the isolated EVs were similar among the tested methods. UF recovered the highest number of EVs followed by PBP, while UC and PBP+SP were significantly less efficient (P<0.05). Based on the number of EVs-to-protein ratios, PBP had the least protein contamination, significantly better than the other methods (P<0.05). All the isolated EVs expressed exosome-enriched proteins CD63, TSG101 and HSP70. Incubation of the trophoblast JEG-3 cells with an equal amount of the fluorescence-labelled EVs isolated by the studied methods showed that many of the PBP-EVs treated cells were fluorescence positive but only a few cells were labelled in the UC- and UF-EVs treated groups. Moreover, the PBP-EVs could transfer significantly more miRNA to the recipient cells than the other 3 methods (P<0.05). The PBP method could isolate EVs from mouse ULF; the diameter of the isolated EVs was 62±19 nm and expressed CD63, TSG101 and HSP70 proteins. In conclusion, PBP could best preserve the activities of the isolated EVs among the 4 methods studied and was able to isolate EVs from a small volume of sample. The simple setup and low equipment demands makes PBP the most suitable method for rapid EV assessment and isolation of EVs in clinical and basic research settings.
[Mh] Termos MeSH primário: Vesículas Extracelulares/metabolismo
Polímeros/química
[Mh] Termos MeSH secundário: Compostos de Anilina/farmacologia
Animais
Compostos de Benzilideno/farmacologia
Blastocisto/citologia
Blastocisto/metabolismo
Linhagem Celular
Precipitação Química
Técnicas de Cocultura
Proteínas de Ligação a DNA/metabolismo
Neoplasias do Endométrio/metabolismo
Neoplasias do Endométrio/patologia
Complexos Endossomais de Distribuição Requeridos para Transporte/metabolismo
Exossomos/efeitos dos fármacos
Exossomos/metabolismo
Vesículas Extracelulares/ultraestrutura
Feminino
Proteínas de Choque Térmico HSP70/metabolismo
Seres Humanos
Camundongos
MicroRNAs/metabolismo
Microscopia Eletrônica
Microscopia de Fluorescência
Tetraspanina 30/metabolismo
Fatores de Transcrição/metabolismo
Trofoblastos/citologia
Trofoblastos/metabolismo
Ultracentrifugação
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Benzylidene Compounds); 0 (DNA-Binding Proteins); 0 (Endosomal Sorting Complexes Required for Transport); 0 (GW 4869); 0 (HSP70 Heat-Shock Proteins); 0 (MicroRNAs); 0 (Polymers); 0 (Tetraspanin 30); 0 (Transcription Factors); 0 (Tsg101 protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186534


  7 / 16506 MEDLINE  
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[PMID]:28850891
[Au] Autor:Satomi Y; Hirayama M; Kobayashi H
[Ad] Endereço:Integrated Technology Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited, Japan. Electronic address: yoshinori.satomi@takeda.com.
[Ti] Título:One-step lipid extraction for plasma lipidomics analysis by liquid chromatography mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:93-100, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the past decade, various lipidomics methodologies have been developed using mass spectrometry based analytical technologies, enabling wide coverage lipid detection in a quantitative manner. Hence, lipidomics has become a widely-accepted approach for biomarker discovery and mechanism elucidation in both medical and biology research fields; however, there are still technical challenges. In this study, focusing on the sample preparation procedure, a single step deproteinization by a water-soluble organic solvent, such as methanol (MeOH), ethanol (EtOH), isopropanol (IPA) or acetonitrile (ACN), was evaluated and proved to be satisfactory for lipidomics analysis. Moreover, during this investigation ACN deproteinization was revealed to not be an effective method for lipid extraction because lipid decomposition was observed during the protein precipitation process through lipase activation, potentially due to the insufficient protein denaturation. Therefore, excluding ACN, protein precipitation by alcohol was evaluated as the lipid extraction reagent. Moreover, adding the MTBE-MeOH (mMM) method, one of the major liquid-liquid extraction methods for shotgun lipidomics, these four approaches were compared. Lipids were extracted from mouse plasma by these four methods and used for exhaustive lipid profiling by liquid chromatography mass spectrometry (LC/MS) analysis. Comparison of these four methods revealed that alcohol based protein precipitation was a useful sample preparation procedure for LC/MS based lipidomics analysis. Whereas MeOH extraction was appropriate for hydrophilic lipid species, IPA was effective for hydrophobic lipids such as triacylglycerols (TG). In practice, EtOH extraction is thought to be the best approach to cover wide range of lipid species using a simple preparation procedure.
[Mh] Termos MeSH primário: Fracionamento Químico/métodos
Cromatografia Líquida/métodos
Lipídeos/sangue
Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Acetonitrilos
Animais
Precipitação Química
Ácidos Cólicos
Gangliosídeos
Lipídeos/química
Lipídeos/isolamento & purificação
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Cholic Acids); 0 (Gangliosides); 0 (Lipids); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


  8 / 16506 MEDLINE  
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[PMID]:28778001
[Au] Autor:Hu H; Li X; Huang P; Zhang Q; Yuan W
[Ad] Endereço:School of Resources & Environmental Engineering, Wuhan University of Technology, 430070 Wuhan, China.
[Ti] Título:Efficient removal of copper from wastewater by using mechanically activated calcium carbonate.
[So] Source:J Environ Manage;203(Pt 1):1-7, 2017 Dec 01.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Copper removal from aqueous solution is necessary from the stances of both environmental protection and copper resource recycling. It is important to develop a new chemical precipitation method suitable for removing copper particularly at low concentration as the case of waste mine water, with regards to the various problems related to the current precipitation methods by using strong alkalis or soluble sulfides. In this research, we studied a possible chemical precipitation of copper ions at concentration around 60 mg/L or lower by cogrinding copper sulfate in water with calcium carbonate (CaCO ) using wet stirred ball milling. With the aid of ball milling, copper precipitation as a basic sulfate (posnjakite: Cu (SO ) (OH) ·H O) occurred at a very high copper removal rate of 99.76%, to reduce the residual copper concentration in the solution less than 0.5 mg/L, reaching the discharge limit, even with the addition amount of CaCO as a stoichiometric ratio of CaCO /Cu at 1:1. It is more interesting to notice that, at the same conditions, other heavy metals such as Ni, Mn, Zn and Cd do not precipitate obviously just with CaCO addition at CaCO /M at 1:1 so that the precipitate without the impurities can be processed as good source to recover copper. This newly proposed concept can be further developed to treat wastewaters with other metals to serve both purposes of environmental purification and resource recovery in a similar way.
[Mh] Termos MeSH primário: Cobre
Águas Residuais
Purificação da Água
[Mh] Termos MeSH secundário: Carbonato de Cálcio
Precipitação Química
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 789U1901C5 (Copper); H0G9379FGK (Calcium Carbonate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  9 / 16506 MEDLINE  
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[PMID]:28652759
[Au] Autor:Fernández-Cidón B; Padró-Miquel A; Alía-Ramos P; Castro-Castro MJ; Fanlo-Maresma M; Dot-Bach D; Valero-Politi J; Pintó-Sala X; Candás-Estébanez B
[Ad] Endereço:Clinical Laboratory, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Spain.
[Ti] Título:Reference values assessment in a Mediterranean population for small dense low-density lipoprotein concentration isolated by an optimized precipitation method.
[So] Source:Vasc Health Risk Manag;13:201-207, 2017.
[Is] ISSN:1178-2048
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: High serum concentrations of small dense low-density lipoprotein cholesterol (sd-LDL-c) particles are associated with risk of cardiovascular disease (CVD). Their clinical application has been hindered as a consequence of the laborious current method used for their quantification. OBJECTIVE: Optimize a simple and fast precipitation method to isolate sd-LDL particles and establish a reference interval in a Mediterranean population. MATERIALS AND METHODS: Forty-five serum samples were collected, and sd-LDL particles were isolated using a modified heparin-Mg precipitation method. sd-LDL-c concentration was calculated by subtracting high-density lipoprotein cholesterol (HDL-c) from the total cholesterol measured in the supernatant. This method was compared with the reference method (ultracentrifugation). Reference values were estimated according to the Clinical and Laboratory Standards Institute and The International Federation of Clinical Chemistry and Laboratory Medicine recommendations. sd-LDL-c concentration was measured in serums from 79 subjects with no lipid metabolism abnormalities. RESULTS: The Passing-Bablok regression equation is = 1.52 (0.72 to 1.73) + 0.07 (-0.1 to 0.13), demonstrating no significant statistical differences between the modified precipitation method and the ultracentrifugation reference method. Similarly, no differences were detected when considering only sd-LDL-c from dyslipidemic patients, since the modifications added to the precipitation method facilitated the proper sedimentation of triglycerides and other lipoproteins. The reference interval for sd-LDL-c concentration estimated in a Mediterranean population was 0.04-0.47 mmol/L. CONCLUSION: An optimization of the heparin-Mg precipitation method for sd-LDL particle isolation was performed, and reference intervals were established in a Spanish Mediterranean population. Measured values were equivalent to those obtained with the reference method, assuring its clinical application when tested in both normolipidemic and dyslipidemic subjects.
[Mh] Termos MeSH primário: Análise Química do Sangue/métodos
Dislipidemias/sangue
Dislipidemias/diagnóstico
Lipoproteínas LDL/sangue
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Biomarcadores/sangue
Análise Química do Sangue/normas
Precipitação Química
Feminino
Heparina/química
Seres Humanos
Lipoproteínas LDL/isolamento & purificação
Magnésio/química
Masculino
Meia-Idade
Tamanho da Partícula
Guias de Prática Clínica como Assunto
Valor Preditivo dos Testes
Padrões de Referência
Análise de Regressão
Reprodutibilidade dos Testes
Espanha
Ultracentrifugação
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Lipoproteins, LDL); 9005-49-6 (Heparin); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.2147/VHRM.S132475


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[PMID]:28635655
[Au] Autor:van der Meer JY; Kellenbach E; van den Bos LJ
[Ad] Endereço:Development and Technical Support Aspen Oss, Kloosterstraat 6, P.O. Box 98, 5340 AB Oss, The Netherlands. jvandermeer@nl.aspenpharma.com.
[Ti] Título:From Farm to Pharma: An Overview of Industrial Heparin Manufacturing Methods.
[So] Source:Molecules;22(6), 2017 Jun 21.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The purification of heparin from offal is an old industrial process for which commercial recipes date back to 1922. Although chemical, chemoenzymatic, and biotechnological alternatives for this production method have been published in the academic literature, animal-tissue is still the sole source for commercial heparin production in industry. Heparin purification methods are closely guarded industrial secrets which are not available to the general (scientific) public. However by reviewing the academic and patent literature, we aim to provide a comprehensive overview of the general methods used in industry for the extraction of heparin from animal tissue.
[Mh] Termos MeSH primário: Anticoagulantes/isolamento & purificação
Heparina/isolamento & purificação
[Mh] Termos MeSH secundário: Matadouros
Animais
Anticoagulantes/farmacologia
Anticoagulantes/uso terapêutico
Precipitação Química
Fazendas
Heparina/biossíntese
Heparina/química
Heparina/uso terapêutico
Mucosa Intestinal/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anticoagulants); 9005-49-6 (Heparin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE



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