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Pesquisa : E05.196.155.650 [Categoria DeCS]
Referências encontradas : 1427 [refinar]
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  1 / 1427 MEDLINE  
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[PMID]:29367487
[Au] Autor:Lin Y; Xie X; Yuan B; Fu J; Liu L; Tian H; Chen T; He D
[Ad] Endereço:College of Food Science and Engineering, Wuhan polytechnic University.
[Ti] Título:Optimization of Enzymatic Cell Disruption for Improving Lipid Extraction from Schizochytrium sp. through Response Surface Methodology.
[So] Source:J Oleo Sci;67(2):215-224, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:This study is aimed to explore the optimal conditions of cell disruption in the extraction algae oil process, using alkaline protease to disrupt cell of Schizochytrium sp. to extract oil in this paper. The effects of enzymatic lysis temperature, enzymatic lysis time, enzyme dosage and pH value on oil yield and DHA yield were studied. Through the combination of single factor test and response surface design, the optimal cell disruption conditions were screened out. The fatty acid composition of algal oil was analyzed by gas chromatography-massspectrometry (GC-MS). The results showed that when the conditions were: enzymatic lysis temperature 55°C, enzymatic lysis time 9 h, enzyme dosage 3% of biomass and pH 8,oil yield and DHA yield reached the highest 14.52 g/L and 7.12 g/L, respectively. When the strains were cultured in 50 L fermentor, oil yield reached 26.27 g/L and DHA yield reached 12.89 g/L. They were 1.81 times higher than that in shake-flask cultivation. The optimization experiment provides the basis for the industrial production of Schizochytrium sp.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Endopeptidases
Extração Líquido-Líquido/métodos
Óleos/isolamento & purificação
Estramenópilas/química
[Mh] Termos MeSH secundário: Ácidos Graxos/análise
Cromatografia Gasosa-Espectrometria de Massas
Concentração de Íons de Hidrogênio
Óleos/química
Estramenópilas/citologia
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fatty Acids); 0 (Oils); EC 3.4.- (Endopeptidases); EC 3.4.99.- (alkaline protease)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17166


  2 / 1427 MEDLINE  
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[PMID]:29367480
[Au] Autor:Mingyai S; Srikaeo K; Kettawan A; Singanusong R; Nakagawa K; Kimura F; Ito J
[Ad] Endereço:Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University.
[Ti] Título:Effects of Extraction Methods on Phytochemicals of Rice Bran Oils Produced from Colored Rice.
[So] Source:J Oleo Sci;67(2):135-142, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Rice bran oil (RBO) especially from colored rice is rich in phytochemicals and has become popular in food, cosmetic, nutraceutical and pharmaceutical applications owing to its offering health benefits. This study determined the contents of phytochemicals including oryzanols, phytosterols, tocopherols (Toc) and tocotrienols (T3) in RBOs extracted using different methods namely cold-press extraction (CPE), solvent extraction (SE) and supercritical CO extraction (SC-CO ). Two colored rice, Red Jasmine rice (RJM, red rice) and Hom-nin rice (HN, black rice), were studied in comparison with the popular Thai fragrant rice Khao Dawk Mali 105 (KDML 105, white rice). RBOs were found to be the rich source of oryzanols, phytosterols, Toc and T3. Rice varieties had a greater effect on the phytochemicals concentrations than extraction methods. HN rice showed the significantly highest concentration of all phytochemicals, followed by RJM and KDML 105 rice, indicating that colored rice contained high concentration of phytochemicals in the oil than non-colored rice. The RBO samples extracted by the CPE method had a greater concentration of the phytochemicals than those extracted by the SC-CO and SE methods, respectively. In terms of phytochemical contents, HN rice extracted using CPE method was found to be the best.
[Mh] Termos MeSH primário: Extração Líquido-Líquido/métodos
Oryza/química
Compostos Fitoquímicos/isolamento & purificação
Óleo de Farelo de Arroz/análise
[Mh] Termos MeSH secundário: Fenilpropionatos/análise
Fenilpropionatos/isolamento & purificação
Compostos Fitoquímicos/análise
Fitosteróis/análise
Fitosteróis/isolamento & purificação
Tocoferóis/análise
Tocoferóis/isolamento & purificação
Tocotrienóis/análise
Tocotrienóis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenylpropionates); 0 (Phytochemicals); 0 (Phytosterols); 0 (Tocotrienols); LZO6K1506A (Rice Bran Oil); R0ZB2556P8 (Tocopherols); SST9XCL51M (gamma-oryzanol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17122


  3 / 1427 MEDLINE  
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[PMID]:29367478
[Au] Autor:Sun M; Xu X; Zhang Q; Rui X; Wu J; Dong M
[Ad] Endereço:College of Food Science and Technology, Nanjing Agricultural University.
[Ti] Título:Ultrasonic-assisted Aqueous Extraction and Physicochemical Characterization of Oil from Clanis bilineata.
[So] Source:J Oleo Sci;67(2):151-165, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Ultrasound-assisted aqueous extraction (UAAE) was used to extract oil from Clanis bilineata (CB), a traditional edible insect that can be reared on a large scale in China, and the physicochemical property and antioxidant capacity of the UAAE-derived oil (UAAEO) were investigated for the first time. UAAE conditions of CB oil was optimized using response surface methodology (RSM) and the highest oil yield (19.47%) was obtained under optimal conditions for ultrasonic power, extraction temperature, extraction time, and ultrasonic interval time at 400 W, 40°C, 50 min, and 2 s, respectively. Compared with Soxhlet extraction-derived oil (SEO), UAAEO had lower acid (AV), peroxide (PV) and p-anisidine values (PAV) as well as higher polyunsaturated fatty acids contents and thermal stability. Furthermore, UAAEO showed stronger antioxidant activities than those of SEO, according to DPPH radical scavenging and ß-carotene bleaching tests. Therefore, UAAE is a promising process for the large-scale production of CB oil and CB has a developing potential as functional oil resource.
[Mh] Termos MeSH primário: Fenômenos Químicos
Larva/química
Lepidópteros/química
Extração Líquido-Líquido/métodos
Óleos/química
Óleos/isolamento & purificação
Ultrassom
Água
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Ácidos Graxos Insaturados/análise
Óleos/farmacologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fatty Acids, Unsaturated); 0 (Oils); 059QF0KO0R (Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17108


  4 / 1427 MEDLINE  
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[PMID]:28749489
[Au] Autor:Stragierowicz J; Daragó A; Brzeznicki S; Kilanowicz A
[Ad] Endereço:Medical University of Lodz, Lódz, Poland (Faculty of Pharmacy, Department of Toxicology). joanna.stragierowicz@umed.lodz.pl.
[Ti] Título:Optimization of ultra-performance liquid chromatography (UPLC) with fluorescence detector (FLD) method for the quantitative determination of selected neurotransmitters in rat brain.
[Ti] Título:Optimization of ultra-performance liquid chromatography (UPLC) with fluorescence detector (FLD) method for the quantitative determination of selected neurotransmitters in rat brain..
[So] Source:Med Pr;68(5):583-591, 2017 Jul 26.
[Is] ISSN:0465-5893
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Glutamate (Glu) and γ-aminobutyric acid (GABA) are the main neurotransmitters in the central nervous system for excitatory and inhibitory processes, respectively. Monitoring these neurotransmitters is an essential tool in establishing pathological functions, among others in terms of occupational exposure to toxic substances. MATERIAL AND METHODS: We present modification of the HPLC (high-performance liquid chromatography) to the UPLC (ultra-performance liquid chromatography) method for the simultaneous determination of glutamate and γ-aminobutyric acid in a single injection. The isocratic separation of these neurotransmitter derivatives was performed on Waters Acquity BEH (ethylene bridged hybrid) C18 column with particle size of 1.7 µm at 35°C using a mobile phase consisting of 0.1 M acetate buffer (pH 6.0) and methanol (60:40, v/v) at a flow rate of 0.3 ml/min. The analytes were detected with the fluorescence detector (FLD) using derivatization with o-phthaldialdehyde (OPA), resulting in excitation at 340 nm and emission at 455 nm. RESULTS: Several validation parameters including linearity (0.999), accuracy (101.1%), intra-day precision (1.52-1.84%), inter-day precision (2.47-3.12%), limit of detection (5-30 ng/ml) and quantification (100 ng/ml) were examined. The developed method was also used for the determination of these neurotransmitters in homogenates of selected rat brain structures. CONCLUSIONS: The presented UPLC-FLD is characterized by shorter separation time (3.5 min), which is an adaptation of the similar HPLC methods and is an alternative for more expensive references techniques such as liquid chromatography coupled with tandem mass-spectrometry (LC-MS/MS) methods. Med Pr 2017;68(5):583-591.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Ácido Glutâmico/análise
Extração Líquido-Líquido/métodos
Neurotransmissores/análise
Ácido gama-Aminobutírico/análise
[Mh] Termos MeSH secundário: Animais
Ratos
Padrões de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotransmitter Agents); 3KX376GY7L (Glutamic Acid); 56-12-2 (gamma-Aminobutyric Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  5 / 1427 MEDLINE  
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[PMID]:29247927
[Au] Autor:Tu X; Sun F; Wu S; Liu W; Gao Z; Huang S; Chen W
[Ad] Endereço:College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, China; MOE Engineering Research Center of Bee Products Processing and Application, Fujian Agriculture and Forestry University, Fuzhou, China.
[Ti] Título:Comparison of salting-out and sugaring-out liquid-liquid extraction methods for the partition of 10-hydroxy-2-decenoic acid in royal jelly and their co-extracted protein content.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:90-95, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Homogeneous liquid-liquid extraction (h-LLE) has been receiving considerable attention as a sample preparation method due to its simple and fast partition of compounds with a wide range of polarities. To better understand the differences between the two h-LLE extraction approaches, salting-out assisted liquid-liquid extraction (SALLE) and sugaring-out assisted liquid-liquid extraction (SULLE), have been compared for the partition of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly, and for the co-extraction of proteins. Effects of the amount of phase partition agents and the concentration of acetonitrile (ACN) on the h-LLE were discussed. Results showed that partition efficiency of 10-HDA depends on the phase ratio in both SALLE and SULLE. Though the partition triggered by NaCl and glucose is less efficient than MgSO in the 50% (v/v) ACN-water mixture, their extraction yields can be improved to be similar with that in MgSO SALLE by increasing the initial concentration of ACN in the ACN-water mixture. The content of co-extracted protein was correlated with water concentration in the obtained upper phase. MgSO showed the largest protein co-extraction at the low concentration of salt. Glucose exhibited a large protein co-extraction in the high phase ratio condition. Furthermore, NaCl with high initial ACN concentration is recommended because it produced high extraction yield for 10-HDA and the lowest amount of co-extracted protein. These observations would be valuable for the sample preparation of royal jelly.
[Mh] Termos MeSH primário: Ácidos Graxos Monoinsaturados/isolamento & purificação
Ácidos Graxos/química
Glucose/química
Extração Líquido-Líquido/métodos
Proteínas/isolamento & purificação
Cloreto de Sódio/química
[Mh] Termos MeSH secundário: Acetonitrilos
Ácidos Graxos Monoinsaturados/análise
Proteínas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Fatty Acids); 0 (Fatty Acids, Monounsaturated); 0 (Proteins); 451W47IQ8X (Sodium Chloride); 765-01-5 (10-hydroxy-2-decenoic acid); IY9XDZ35W2 (Glucose); L497I37F0C (royal jelly); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


  6 / 1427 MEDLINE  
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[PMID]:29241086
[Au] Autor:Hameed R; Huddleston J; Ignatova S
[Ad] Endereço:Advanced Bioprocessing Centre, Institute of Environment, Health and Societies, College of Engineering, Design and Physical Sciences, Brunel University London, Uxbridge, UB8 3PH, UK.
[Ti] Título:Practical aspects of the automated preparation of aqueous two phase systems for the analysis of biological macromolecules.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:60-68, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A robust strategy for the automated preparation of aqueous two-phase systems (ATPS) using a liquid handling sample processor was developed using gravimetric methods: to determine the accuracy of preparation. The major robotic control parameters requiring adjustment were; speed of aspiration and dispense; delay times following aspiration and dispense alongside measures to control cross-contamination during phase sampling. In general mixture compositions of both polymer/polymer and polymer/salt mixtures could be prepared with a target bias accuracy of less than 5%. However, we found that the bias accuracy with which systems of defined TLL and MR could be constructed was highly dependent on the tie line length of the ATPS and the geometrical form of the ATPS co-existence curve. For systems with a very low degree of curvature (PEG/salt systems here) increases in bias (accuracy) are appreciable at relatively long tie line lengths. Where the degree of curvature is more pronounced (PEG/dextran systems) closer approach to the critical point was possible without major effect on bias/accuracy. Application of the strategy to the measurement of the partitioning of phosphorylated and dephosphorylated forms of the model protein ovalbumin are reported. Differences in partition of phosphorylated (native) forms and dephosphorylated forms could be demonstrated. In a PEG/salt system this was manifest as a substantial decrease in solubility based on overall protein recovery derived from accurate knowledge of the system mass ratio. In a PEG/dextran system differences in partition coefficient could be demonstrated between phosphorylated and dephosphorylated forms.
[Mh] Termos MeSH primário: Automação Laboratorial/métodos
Cromatografia Líquida/métodos
Extração Líquido-Líquido/métodos
Modelos Químicos
[Mh] Termos MeSH secundário: Ovalbumina/análise
Ovalbumina/química
Ovalbumina/isolamento & purificação
Isoformas de Proteínas/análise
Isoformas de Proteínas/química
Isoformas de Proteínas/isolamento & purificação
Proteínas Recombinantes/análise
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Robótica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Isoforms); 0 (Recombinant Proteins); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  7 / 1427 MEDLINE  
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[PMID]:29241085
[Au] Autor:Alshishani A; Salhimi SM; Saad B
[Ad] Endereço:School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia. Electronic address: anasshishani@gmail.com.
[Ti] Título:Salting-out assisted liquid-liquid extraction coupled with hydrophilic interaction chromatography for the determination of biguanides in biological and environmental samples.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:51-59, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new salting-out assisted liquid-liquid extraction (SALLE) sample preparation method for the determination of the polar anti-diabetic biguanide drugs (metformin, buformin and phenformin) in blood plasma, urine and lake water samples were developed. The SALLE was performed by mixing samples (plasma (0.2mL), urine or lake water (1.0mL)) with acetonitrile (0.4mL for plasma, 0.5mL for urine or lake water), sodium hydroxide powder was then added for the phase separation. The effects of type of salting-out reagent, type of extraction solvent, volumes of acetonitrile and sample, amount of sodium hydroxide, vortexing and centrifugation times on the extraction efficiency were investigated. The upper layer, containing the biguanides, was directly injected into a HPLC unit using ZIC-HILIC column (150mm×2.1mm×3.5µm) and was detected at 236nm. The method was validated and calibration curves were linear with r >0.99 over the range of 20-2000µgL for plasma and 5-2000µgL for urine and lake water samples. The limits of detection were in the range (3.8-5.6)µgL , (0.8-1.5)µgL and (0.3-0.8)µgL for plasma, urine and lake water, respectively. The accuracies in the three matrices were within 87.3-103%, 87.4-109%, 82.2-109% of the nominal concentration for metformin, buformin and phenformin, respectively. The relative standard deviation for inter- and intra -day precision were in the range of 1.0-17% for all analytes in the three matrices.
[Mh] Termos MeSH primário: Biguanidas/análise
Biguanidas/isolamento & purificação
Cromatografia Líquida de Alta Pressão/métodos
Extração Líquido-Líquido/métodos
Poluentes Químicos da Água/análise
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Acetonitrilos
Biguanidas/química
Biguanidas/urina
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Lagos/química
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Cloreto de Sódio
Poluentes Químicos da Água/química
Poluentes Químicos da Água/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Biguanides); 0 (Water Pollutants, Chemical); 451W47IQ8X (Sodium Chloride); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  8 / 1427 MEDLINE  
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[PMID]:29223048
[Au] Autor:Zheng N; Lian B; Du W; Xu G; Ji J
[Ad] Endereço:Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), National Drug Clinical Trial Center, Peking University Cancer Hospital & Institute, Beijing 100142, China.
[Ti] Título:Extraction protocol and liquid chromatography/tandem mass spectrometry method for determining micelle-entrapped paclitaxel at the cellular and subcellular levels: Application to a cellular uptake and distribution study.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:347-354, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Paclitaxel-loaded polymeric micelles (PTX-PM) are commonly used as tumor-targeted nanocarriers and display outstanding antitumor features in clinic, but its accumulation and distribution in vitro are lack of investigation. It is probably due to the complex micellar system and its low concentration at the cellular or subcellular levels. In this study, we developed an improved extraction method, which was a combination of mechanical disruption and liquid-liquid extraction (LLE), to extract the total PTX from micelles in the cell lysate and subcellular compartments. An ultra-performance liquid chromatography tandem mass spectroscopy (UPLC-MS/MS) method was optimized to detect the low concentration of PTX at cellular and subcellular levels simultaneously, using docetaxel as internal standard (IS). The method was proved to release PTX totally from micelles (≥95.93%) with a consistent and reproducible extraction recovery (≥75.04%). Good linearity was obtained at concentrations ranging from 0.2 to 20ng/mL. The relative error (RE%) for accuracy varied from 0.68 to 7.56%, and the intra- and inter-precision (relative standard deviation, RSD%) was less than 8.64% and 13.14%, respectively. This method was fully validated and successfully applied to the cellular uptake and distribution study of PTX-loaded PLGA-PEG micelles in human breast cancer cells (MCF-7).
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Paclitaxel/análise
Paclitaxel/farmacocinética
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Estabilidade de Medicamentos
Seres Humanos
Limite de Detecção
Modelos Lineares
Extração Líquido-Líquido
Células MCF-7
Micelas
Paclitaxel/química
Paclitaxel/isolamento & purificação
Poliésteres/química
Polietilenoglicóis/química
Reprodutibilidade dos Testes
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Micelles); 0 (Polyesters); 0 (polyethylene glycol-poly(lactide-co-glycolide)); 30IQX730WE (Polyethylene Glycols); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  9 / 1427 MEDLINE  
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[PMID]:29300475
[Au] Autor:Zou D; Zhu X; Zhang F; Du Y; Ma J; Jiang R
[Ad] Endereço:Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University , Guangzhou 510632, P. R. China.
[Ti] Título:An Efficient Strategy Based on Liquid-Liquid Extraction with Three-Phase Solvent System and High Speed Counter-Current Chromatography for Rapid Enrichment and Separation of Epimers of Minor Bufadienolide from Toad Meat.
[So] Source:J Agric Food Chem;66(4):1008-1014, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study presents an efficient strategy based on liquid-liquid extraction with three-phase solvent system and high speed counter-current chromatography for rapid enrichment and separation of epimers of minor bufadienolide from toad meat. The reflux extraction conditions were optimized by response surface methodology first, and a novel three-phase solvent system composed of n-hexane/methyl acetate/acetonitrile/water (3:6:5:5, v/v) was developed for liquid-liquid extraction of the crude extract. This integrative extraction process could enrich minor bufadienolide from complex matrix efficiently and minimize the loss of minor targets induced by repeated extraction with different kinds of organic solvents occurring in the classical liquid two-phase extraction. As a result, four epimers of minor bufadienolide were greatly enriched in the middle phase and total content of these epimers of minor bufadienolide was increased from 3.25% to 46.23%. Then, the enriched four epimers were separated by HSCCC with a two-phase solvent system composed of chloroform/methanol/water (4:2:2, v/v) successfully. Furthermore, we tested Na ,K -ATPase (NKA) inhibitory effect of the four epimers. 3ß-Isomers of bufadienolide showed stronger (>8-fold) inhibitory activity than 3α-isomers. The characterization of minor bufadienolide in toad meat and their significant difference of inhibitory effect on NKA would promote the further quantitative analysis and safety evaluation of toad meat as a food source.
[Mh] Termos MeSH primário: Bufanolídeos/química
Bufanolídeos/isolamento & purificação
Bufonidae
Distribuição Contracorrente/métodos
Extração Líquido-Líquido/métodos
Carne/análise
[Mh] Termos MeSH secundário: Animais
Bufanolídeos/farmacologia
Inibidores Enzimáticos
Isomerismo
ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bufanolides); 0 (Enzyme Inhibitors); 0 (Solvents); 29565-35-3 (bufadienolide); EC 3.6.1.- (ATP1A1 protein, human); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05310


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Fotocópia
[PMID]:29364597
[Au] Autor:Solov'ev AA; Ashikhmin AA; Moskalenko AA
[Ti] Título:Formation of a Subunit Form of the Core Light-Harvesting Complex from Sulfur Purple Bacteria Ectothiorhodospira haloalkaliphila with Different Carotenoid Composition.
[So] Source:Mikrobiologiia;85(5):497-505, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:B820 subunits from a purple sulfur bacterium Ectothiorhodospira. haloalkaliphila strain ATCC 51935T were obtained by treatment of Carotenoid free LH I-RC complexes of this bacterium with P--octylglu- copyranoside (ß-OG). The same complexes with 100% carotenoid content were unable to dissociate to B820 subunits, but disintegrated to monomeric bacteriochlorophyll (BChl) regardless of their carotenoid compo- sition. The degree of dissociation of the LH 1-RC complexes with an intermediate content of carotenoids (the' B820 formation) was directly dependent on the quantity of carotenoids in the samples. The resulting B820 subunits did not contain carotenoids. B820 subunits easily aggregated to form a complex with an absorption . peak at 880 nm at decreased ß-OG concentration. Analysis of the spectra of the LH I-RC complexes isolated from the cells with different'levels of carotenogenesis inhibition led to the conclusion of the heterogeneity of the samples with a predominance in them of (a) the fraction with 100% of carotenoids and (b) the fraction of carotenoid free complexes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Carotenoides/isolamento & purificação
Chromatiaceae/química
Ectothiorhodospiraceae/química
Complexos de Proteínas Captadores de Luz/química
Subunidades Proteicas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/isolamento & purificação
Bacterioclorofilas/química
Bacterioclorofilas/isolamento & purificação
Carotenoides/química
Carotenoides/classificação
Chromatiaceae/metabolismo
Detergentes/química
Ectothiorhodospiraceae/metabolismo
Glucosídeos/química
Complexos de Proteínas Captadores de Luz/isolamento & purificação
Extração Líquido-Líquido/métodos
Agregados Proteicos
Subunidades Proteicas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriochlorophylls); 0 (Detergents); 0 (Glucosides); 0 (Light-Harvesting Protein Complexes); 0 (Protein Aggregates); 0 (Protein Subunits); 29836-26-8 (octyl-beta-D-glucoside); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE



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