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[PMID]:29446274
[Au] Autor:Bragina IV; Fedorova NE; Volkova VN; Egorchenkova OE; Mukhina LP; Larkina MV
[Ti] Título:[The method of multicomponent determination of herbicides of various chemical classes in water].
[So] Source:Gig Sanit;95(11):1099-104, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:In the work there are considered results of the development of the multicomponent method of measurement of concentration of herbicides of various chemical nature under their joint presence in the water. There was justified the optimality of application of HPLC-DAD (the working wavelength of 240 nm) for the determination of levels of 10 active ingredients of herbicides of class of sulfonylurea (metsulfuron-methyl, nikosulfuron, sulfometuron-methyl, thifensulfuron-methyl, triflusulfuron-methyl), imidazolinone (imazapyr, imazethapyr), 2,6-Bis[(4,6-dimethoxy-2- pyrimidinyl)oxy]benzoic acid (bispyribac acid), triazol-pyrimidines (Penoxsulam), a benzoylpyrazole compound (Topramenzone). For the concentrating and cleaning of samples of water there were used cartridges for solid-phase extraction of Oasis HLB - the macro porous copolymer made on the basis of the balanced ratio of 2 monomers - lipophilic divinylbenzene and hydrophilic N-vinylpirrolidone. The range of the detected concentrations in water was volatile between 0.0005 and 0.005 mg/L, values of standard deviation vary in the range of 1.8-3.9%. Chlorine-containing acidic herbicides were analyzed by the method of GC-ECD and GC-MS (IE) after preliminary converting of compounds into flying derivatives with the use of diazomethane. Satisfactory extraction of substances from a water sample may be achieved by classical extraction in the system "liquid-liquid" with the application of Methyl tert-butyl ester. For cleaning of the derivatized sample there were used cartridges for solid-phase extraction on the basis of silica gel. The range of the determination of 9 active ingredients referring to classes of phenoxy-acetic acid (2,4- D, MCPA), pyridinecarboxylic (aminopyiralid, picloram, clopyralid), benzoic acids (dicamba), benzothiadiazinone (bentazone), biphenyl ester (acifluorfen) and a chloroacetamide (acetochlor) - 0.0001-0.001 mg/L, SD values vary in the range of 1.8-33%.
[Mh] Termos MeSH primário: Cromatografia Gasosa/métodos
Cromatografia de Fase Reversa/métodos
Herbicidas
[Mh] Termos MeSH secundário: Monitoramento Ambiental/métodos
Herbicidas/análise
Herbicidas/classificação
Seres Humanos
Reprodutibilidade dos Testes
Poluição Química da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


  2 / 2731 MEDLINE  
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[PMID]:29396367
[Au] Autor:Police A; Shankar VK; Narasimha Murthy S
[Ad] Endereço:Department of Pharmaceutics and Drug Delivery, University of Mississippi, MS 38677, USA.
[Ti] Título:RP-HPLC method for simultaneous estimation of vigabatrin, gamma-aminobutyric acid and taurine in biological samples.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:44-53, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Vigabatrin is used as first line drug in treatment of infantile spasms for its potential benefit overweighing risk of causing permanent peripheral visual field defects and retinal damage. Chronic administration of vigabatrin in rats has demonstrated these ocular events are result of GABA accumulation and depletion of taurine levels in retinal tissues. In vigabatrin clinical studies taurine plasma level is considered as biomarker for studying structure and function of retina. The analytical method is essential to monitor taurine levels along with vigabatrin and GABA. A RP-HPLC method has been developed and validated for simultaneous estimation of vigabatrin, GABA and taurine using surrogate matrix. Analytes were extracted from human plasma, rat plasma, retina and brain by simple protein precipitation method and derivatized by naphthalene 2, 3­dicarboxaldehyde to produce stable fluorescent active isoindole derivatives. The chromatographic analysis was performed on Zorbax Eclipse AAA column using gradient elution profile and eluent was monitored using fluorescence detector. A linear plot of calibration curve was observed in concentration range of 64.6 to 6458, 51.5 to 5150 and 62.5 to 6258 ng/mL for vigabatrin, GABA and taurine, respectively with r ≥ 0.997 for all analytes. The method was successfully applied for estimating levels of vigabatrin and its modulator effect on GABA and taurine levels in rat plasma, brain and retinal tissue. This RP-HPLC method can be applied in clinical and preclinical studies to explore the effect of taurine deficiency and to investigate novel approaches for alleviating vigabatrin induced ocular toxicity.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Taurina/análise
Vigabatrina/análise
Ácido gama-Aminobutírico/análise
[Mh] Termos MeSH secundário: Animais
Química Encefálica
Cromatografia de Fase Reversa/métodos
Seres Humanos
Modelos Lineares
Masculino
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Retina/química
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1EQV5MLY3D (Taurine); 56-12-2 (gamma-Aminobutyric Acid); GR120KRT6K (Vigabatrin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


  3 / 2731 MEDLINE  
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[PMID]:29229336
[Au] Autor:Panda SK; Muller H; Al-Qunaysi TA; Koseoglu OR
[Ad] Endereço:Saudi Aramco, Research & Development Center, 31311, Dhahran, Saudi Arabia. Electronic address: saroj.panda@aramco.com.
[Ti] Título:Determination of heavy polycyclic aromatic hydrocarbons by non-aqueous reversed phase liquid chromatography: Application and limitation in refining streams.
[So] Source:J Chromatogr A;1533:30-37, 2018 Jan 19.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The heavy polycyclic aromatic hydrocarbons (HPAHs) cause detrimental effects to hydrocracker operations by deactivating the catalysts and depositing in the downstream of the reactor/ exchangers. Therefore, it is essential to continuously monitor the accumulation of HPAHs in a hydrocracker unit. To accurately measure the concentration of HPAHs, the development of a fast and reliable analytical method is inevitable. In this work, an analytical method based on non-aqueous reversed phase chromatography in combination with high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was developed. As a first step, five different types of stationary phases were evaluated for the separation of HPAHs in non-aqueous mode and the best suited phase was further used for the fractionation of HPAHs in a fractionator bottom sample obtained from a refinery hydrocracker unit. The eight major fractions or peaks obtained from the separation were further characterized by UV spectroscopy and FT-ICR MS and the compounds in the fractions were tentatively confirmed as benzoperylene, coronene, methylcoronene, naphthenocoronene, benzocoronene, dibenzoperylene, naphthocoronene and ovalene. The developed liquid chromatography method can be easily adapted in a refinery laboratory for the quantitation of HPAHs in hydrocracking products. The method was further tested to check the interference of sulfur aromatics and/or large alkylated aromatic hydrocarbons on the determination of HPAHs in hydrocracking products.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Cromatografia de Fase Reversa
Espectrometria de Massas
Indústria de Petróleo e Gás/métodos
Hidrocarbonetos Aromáticos Policíclicos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  4 / 2731 MEDLINE  
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[PMID]:29191406
[Au] Autor:Ghanem A; Wang C
[Ad] Endereço:Chirality Program, Faculty of Science and Technology, University of Canberra, ACT, 2601, Australia. Electronic address: ashraf.ghanem@canberra.edu.au.
[Ti] Título:Enantioselective separation of racemates using CHIRALPAK IG amylose-based chiral stationary phase under normal standard, non-standard and reversed phase high performance liquid chromatography.
[So] Source:J Chromatogr A;1532:89-97, 2018 Jan 12.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We have previously reported on the solvent versatility of immobilized amylose and cellulose-based chiral stationary phases in enantioselective liquid chromatographic separation of racemates. The studies were mainly focusing on the tris substituted 3,5-dimethylphenylcarbamate polysaccharide-based chiral stationary phases namely CHIRALPAK IA [Amylose tris (3,5-dimethylphenylcarbamate)] or ADMPC and CHIRALPAK IB [Cellulose tris (3,5-dimethylphenylcarbamate)] or CDMPC. Here we focus on the application of the recently introduced amylose tris (3-chloro-5-methylphenylcarbamate) or ACMPC and brand name CHIRALPAK IG with a chlorine substituent replacing the methyl group in CHIRALPAK IA . This was investigated for the enantioslective separation of different classes of pharmaceuticals namely ß- and α-blockers, anti-inflammatory and antifungal drugs, norepinephrine-dopamine reuptake inhibitor, catecholamines, sedative hypnotics, anti-histaminics, anticancer drugs, antiarrhythmic drugs, flavonoids, amino acids, alpha-2 adrenergic agonist, adrenaline and miscellaneous.A brief comparison between CHIRALPAK IG and CHIRALPAK IA under normal standard, non-standard and reversed mobile phase is demonstrated. The results revealed the versatility of the CHIRALPAK IG column, its compatibility with a wide ranges of solvent and operation modes and its ability to separate chiral compounds not separated with other amylose based chiral stationary phases.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Cromatografia Líquida de Alta Pressão
Preparações Farmacêuticas/isolamento & purificação
[Mh] Termos MeSH secundário: Amilose/química
Técnicas de Química Analítica/instrumentação
Cromatografia de Fase Reversa
Preparações Farmacêuticas/química
Solventes/química
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pharmaceutical Preparations); 0 (Solvents); 9005-82-7 (Amylose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


  5 / 2731 MEDLINE  
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Bartolini, Paolo
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[PMID]:29179059
[Au] Autor:Dias PVS; Arthuso FS; Oliveira JE; Suzuki MF; Sousa JM; Ribela MTCP; Bartolini P; Soares CRJ
[Ad] Endereço:Biotechnology Center, Instituto de Pesquisas Energéticas e Nucleares, IPEN - CNEN/SP, São Paulo, Brazil.
[Ti] Título:Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:193-198, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Reversed-phase high-performance liquid chromatography (RP-HPLC) has been used to analyze Interferon α-2 (IFN-α2) as a pure protein or as a pharmaceutical preparation: a method for analyzing periplasmic IFN-α2 directly in osmotic shock extract has, however, never been reported. This work describes an RP-HPLC methodology for the qualitative and quantitative analysis of human IFN-α2a and IFN-α2b directly in bacterial periplasmic extracts or in purified preparations. The analytical method has been set up and validated for accuracy, precision, linearity, sensitivity and specificity. A recovery test indicated an average bias of ∼1%, intra-day and inter-day quantitative determinations presented relative standard deviations always≤5%, while the working sensitivity was of ∼0.3µg of IFN-α2 (RSD=5%). The method proved to be suitable for detecting and quantifying also glycosylated and oxidized forms and N-methionylated IFN-α2 molecules, it was, however, not able to distinguish between IFN-α2a and IFN-α2b. This rapid methodology allows the application of RP-HPLC as a powerful tool to monitor the production yield and quality of IFN-α2 in osmotic shock fluids, right after, or even during the fermentation process.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Cromatografia de Fase Reversa/métodos
Escherichia coli/genética
Interferon-alfa/análise
Proteínas Recombinantes/análise
[Mh] Termos MeSH secundário: Glicosilação
Seres Humanos
Interferon-alfa/química
Interferon-alfa/genética
Interferon-alfa/isolamento & purificação
Modelos Lineares
Oxirredução
Periplasma/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IFNA2 protein, human); 0 (Interferon-alpha); 0 (Recombinant Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  6 / 2731 MEDLINE  
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[PMID]:29175698
[Au] Autor:Santos Á; Soares JX; Cravo S; Tiritan ME; Reis S; Afonso C; Fernandes C; Pinto MMM
[Ad] Endereço:Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
[Ti] Título:Lipophilicity assessement in drug discovery: Experimental and theoretical methods applied to xanthone derivatives.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:182-192, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:For the last several years, searching of new xanthone derivatives (XDs) with potential pharmacological activities has remained one of the main areas of interest of our group. The optimization of biological activity and drug-like properties of hits and leads is crucial at early stage of the drug discovery pipeline. Lipophilicity is one of the most important drug-like properties having a great impact in both pharmacokinetics and pharmacodynamics processes. In this work, we describe the lipophilicity of a small library of bioactive XDs, previously synthesized by our group, using different methods: computational, vortex-assisted liquid-liquid microextraction coupled with high-performance liquid chromatography (VALLME-HPLC), reversed-phase high-performance thin layer chromatography (RP-HPTLC), reversed-phase high-performance liquid chromatography (RP-HPLC), and biomembrane model by the partition between micelles and aqueous phase. The different results obtained by the used methods were compared and discussed. The methodologies and data gathered in this study will expand the investigation of lipophilicity of XDs, an important class of compounds in medicinal chemistry.
[Mh] Termos MeSH primário: Descoberta de Drogas/métodos
Xantonas/análise
Xantonas/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Cromatografia de Fase Reversa/métodos
Interações Hidrofóbicas e Hidrofílicas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Xanthones)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  7 / 2731 MEDLINE  
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[PMID]:29175696
[Au] Autor:Bergh MS; Bogen IL; Andersen JM; Øiestad ÅML; Berg T
[Ad] Endereço:Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway; Department of Chemistry, University of Oslo, Oslo, Norway. Electronic address: Marianne.Skov-Skov.Bergh@ous-hf.no.
[Ti] Título:Determination of adrenaline, noradrenaline and corticosterone in rodent blood by ion pair reversed phase UHPLC-MS/MS.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:161-172, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel ion pair reversed phase ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of the stress hormones adrenaline, noradrenaline and corticosterone in rodent blood was developed and fully validated. Separations were performed on an Acquity HSS T3 column (2.1mm i.d.×100mm, 1.8µm) with gradient elution and a runtime of 5.5min. The retention of adrenaline and noradrenaline was substantially increased by employing the ion pair reagent heptafluorobutyric acid (HFBA). Ion pair reagents are usually added to the mobile phase only, but we demonstrate for the first time that including HFBA to the sample reconstitution solvent as well, has a major impact on the chromatography of these compounds. The stability of adrenaline and corticosterone in rodent blood was investigated using the surrogate analytes adrenaline-d and corticosterone-d . The applicability of the described method was demonstrated by measuring the concentration of stress hormones in rodent blood samples.
[Mh] Termos MeSH primário: Cromatografia de Fase Reversa/métodos
Corticosterona/sangue
Epinefrina/sangue
Norepinefrina/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Estabilidade de Medicamentos
Limite de Detecção
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
W980KJ009P (Corticosterone); X4W3ENH1CV (Norepinephrine); YKH834O4BH (Epinephrine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  8 / 2731 MEDLINE  
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[PMID]:29324762
[Au] Autor:Showalter MR; Nonnecke EB; Linderholm AL; Cajka T; Sa MR; Lönnerdal B; Kenyon NJ; Fiehn O
[Ad] Endereço:NIH West Coast Metabolomics Center, University of California Davis, Davis, CA, United States of America.
[Ti] Título:Obesogenic diets alter metabolism in mice.
[So] Source:PLoS One;13(1):e0190632, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects the lung are not well characterized. Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets. Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days. We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition. From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments. Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members. While some metabolites were altered in both obese groups compared to control, others were different between obesogenic diet groups. Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ. These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.
[Mh] Termos MeSH primário: Dieta Hiperlipídica
Sacarose na Dieta/administração & dosagem
Metabolômica
Obesidade/etiologia
[Mh] Termos MeSH secundário: Animais
Cromatografia de Fase Reversa
Metabolismo Energético
Masculino
Espectrometria de Massas
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Dietary Sucrose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190632


  9 / 2731 MEDLINE  
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[PMID]:29216582
[Au] Autor:Fiori J; Amadesi E; Fanelli F; Tropeano CV; Rugolo M; Gotti R
[Ad] Endereço:Department of Pharmacy and Biotechnology, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy. Electronic address: jessica.fiori@unibo.it.
[Ti] Título:Cellular and mitochondrial determination of low molecular mass organic acids by LC-MS/MS.
[So] Source:J Pharm Biomed Anal;150:33-38, 2018 Feb 20.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A selective and sensitive method for the determination of low molecular mass organic acids (LMMOAs) in cell and mitochondrial extracts is presented. The analytical method consists in the separation by reversed phase liquid chromatography and detection with tandem mass spectrometry (LC-MS/MS) of the LMMOAs like malic, succinic, formic and citric acids. These acids are among the cellular intermediates of the tricarboxylic acid cycle (TCA), thus their quantitation can provide essential information about the catabolic and anabolic processes occurring in cells under physiological and pathological conditions. The analytical method was fully validated in terms of linearity, detection and quantification limits, recovery and precision. Detection limits (LOD) for malic, succinic and fumaric acids were in the range of 1-10nM, while 20nM was obtained for citric acid. Analytical recovery in cell and mitochondrial extracts was found between 88 and 105% (CV% ≤7.1) and matrix effect was estimated to be less than 108%. The LC-MS/MS method applied to the quantification of TCA cycle metabolites revealed a different distribution of the four acids in cells and mitochondria, and it could be used to monitoring metabolic alterations associated with TCA cycle and oxidative phosphorylation dysfunctions.
[Mh] Termos MeSH primário: Ácidos Acíclicos/análise
Cromatografia de Fase Reversa/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Ácidos Acíclicos/metabolismo
Ciclo do Ácido Cítrico
Seres Humanos
Limite de Detecção
Mitocôndrias/metabolismo
Peso Molecular
Compostos Orgânicos/análise
Compostos Orgânicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acids, Acyclic); 0 (Organic Chemicals)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  10 / 2731 MEDLINE  
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[PMID]:29179555
[Au] Autor:Ma L; Liu G
[Ad] Endereço:School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
[Ti] Título:Simultaneous Analysis of Malondialdehyde, 4-Hydroxy-2-hexenal, and 4-Hydroxy-2-nonenal in Vegetable Oil by Reversed-Phase High-Performance Liquid Chromatography.
[So] Source:J Agric Food Chem;65(51):11320-11328, 2017 Dec 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A group of toxic aldehydes such as, malondialdehyde (MDA), 4-hydroxy-2-hexenal (HHE), and 4-hydroxy-2-nonenal (HNE) have been found in various vegetable oils and oil-based foods. Then simultaneous determination of them holds a great need in both the oil chemistry field and food field. In the present study, a simple and efficient analytical method was successfully developed for the simultaneous separation and detection of MDA, HHE, and HNE in vegetable oils by reversed-phase-high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detector (PAD) at dual-channel detection mode. The effect of various experimental factors on the extraction performance, such as coextraction solvent system, butylated hydroxytoluene addition, and trichloroacetic acid addition were systematically investigated. Results showed that the linear ranges were 0.02-10.00 µg/mL for MDA, 0.02-4.00 µg/mL for HHE, and 0.03-4.00 µg/mL for HNE with the satisfactory correlation coefficient of >0.999 for all detected aldehydes. The limit of detection (LOD) and limit of quantification (LOQ) of MDA, HHE, and HNE were ∼0.021and 0.020 µg/mL, ∼0.009 and 0.020 µg/mL, and ∼0.014 and 0.030 µg/mL, respectively. Their recoveries were 99.64-102.18%, 102.34-104.61%, and 98.87-103.04% for rapeseed oil and 96.38-98.05%, 96.19-101.34%, and 96.86-99.04% for French fries, separately. Under the selected conditions, the developed methods was successfully applied to the simultaneous determination of MDA, HHE, and HNE in different tested vegetable oils. The results indicated that this method could be employed for the quality assessment of vegetable oils.
[Mh] Termos MeSH primário: Aldeídos/análise
Cromatografia de Fase Reversa/métodos
Malondialdeído/análise
Óleos Vegetais/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Plant Oils); 17427-08-6 (4-hydroxy-2-hexenal); 4Y8F71G49Q (Malondialdehyde); K1CVM13F96 (4-hydroxy-2-nonenal)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04566



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