Base de dados : MEDLINE
Pesquisa : E05.196.353.500 [Categoria DeCS]
Referências encontradas : 10549 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1055 ir para página                         

  1 / 10549 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29324792
[Au] Autor:Cibicek N; Ehrmann J; Proskova J; Vecera R
[Ad] Endereço:Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
[Ti] Título:Critical evaluation of colon submucosal microdialysis in awake, mobile rats.
[So] Source:PLoS One;13(1):e0191041, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sensors able to record large bowel physiology and biochemistry in situ in awake rodents are lacking. Microdialysis is a mini-invasive technique that may be utilized to continuously deliver or recover low-molecular substances from various tissues. In this experiment we evaluated the feasibility of in vivo microdialysis to monitor extracellular fluid chemistry in the descending colon submucosa of conscious, freely moving rodents. Following surgical implantation of a microdialysis probe, male Wistar rats were housed in metabolic cages where they were analgized and clinically followed for four days with free access to standard diet and water. To assess local microcirculation and probe function, glucose, lactate, glucose-to-lactate ratio and urea clearance were determined in the dialysates from the three postoperative days with focus on the final 24-h period. In an attempt to mitigate the expected tissue inflammatory response, one group of animals had the catheters perfused with 5-aminosalicylic acid-enriched medium with final concentration 1 µmol/L. For verification of probe position and the assessment of the surrounding foreign body reaction, standard histological and immunohistochemical methods were employed. Microdialysis of rat gut is associated with considerable technical challenges that may lead to the loss of probe function and high drop-out rate. In this setting, limited data did not allow to draw any firm conclusion regarding local anti-inflammatory effectiveness of 5-aminosalicylic acid perfusion. Although intestinal microdialysis may be suitable for larger anesthetized animals, low reproducibility of the presented method compromises its routine experimental use in awake and freely moving small-sized rodents.
[Mh] Termos MeSH primário: Colo/fisiologia
[Mh] Termos MeSH secundário: Animais
Colo/irrigação sanguínea
Glucose/metabolismo
Mucosa Intestinal/fisiologia
Ácido Láctico/metabolismo
Masculino
Microcirculação
Microdiálise
Ratos
Ratos Wistar
Ureia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
33X04XA5AT (Lactic Acid); 8W8T17847W (Urea); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191041


  2 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29406030
[Au] Autor:Zhang X; Pi Z; Zheng Z; Liu Z; Song F
[Ad] Endereço:National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
[Ti] Título:Comprehensive investigation of in-vivo ingredients and action mechanism of iridoid extract from Gardeniae Fructus by liquid chromatography combined with mass spectrometry, microdialysis sampling and network pharmacology.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:70-76, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gardeniae Fructus is a widely used Traditional Chinese Medicines in treating various diseases. However, the absorbed components and metabolites of its main bioactive iridoid ingredients from iridoid extract of the fruits of Gardeniae Fructus in rat plasma need further study. In this study, a systematic method based on ultra-performance liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) technique was developed to speculate the absorbed components and metabolites of iridoid extract in rat plasma after oral administration. A total of 19 compounds, including 9 prototype components and 10 metabolites were identified in plasma. 5 metabolites containing 4 new metabolites (M1, M2, M7, M10) were tentatively determined in rat plasma. Besides, Microdialysis-intensity-fading mass spectrometry (MD-IF-MS) method was originally employed to reveal the binding affinities with α-glucosidase for in-vivo prototype components and their metabolites. Finally, the absorbed constituents and the corresponding target proteins were used to generate compound-target network to find the related diseases and action pathways by a network pharmacology method. The results provide useful information for further study of pharmacology and in vivo mechanism of action of iridoid extract from the fruits of Gardeniae Fructus.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Gardenia/química
Iridoides/sangue
Iridoides/metabolismo
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Animais
Frutas/química
Masculino
Microdiálise
Extratos Vegetais/metabolismo
Ratos
Ratos Sprague-Dawley
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iridoids); 0 (Plant Extracts)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  3 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27773601
[Au] Autor:Huff CL; Morano RL; Herman JP; Yamamoto BK; Gudelsky GA
[Ad] Endereço:Division of Pharmaceutical Sciences, University of Cincinnati-James Winkle College of Pharmacy, Cincinnati, OH 45267, United States.
[Ti] Título:MDMA decreases glutamic acid decarboxylase (GAD) 67-immunoreactive neurons in the hippocampus and increases seizure susceptibility: Role for glutamate.
[So] Source:Neurotoxicology;57:282-290, 2016 12.
[Is] ISSN:1872-9711
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:3,4-Methylenedioxy-methamphetamine (MDMA) is a unique psychostimulant that continues to be a popular drug of abuse. It has been well documented that MDMA reduces markers of 5-HT axon terminals in rodents, as well as humans. A loss of parvalbumin-immunoreactive (IR) interneurons in the hippocampus following MDMA treatment has only been documented recently. In the present study, we tested the hypothesis that MDMA reduces glutamic acid decarboxylase (GAD) 67-IR, another biochemical marker of GABA neurons, in the hippocampus and that this reduction in GAD67-IR neurons and an accompanying increase in seizure susceptibility involve glutamate receptor activation. Repeated exposure to MDMA (3×10mg/kg, ip) resulted in a reduction of 37-58% of GAD67-IR cells in the dentate gyrus (DG), CA1, and CA3 regions, as well as an increased susceptibility to kainic acid-induced seizures, both of which persisted for at least 30days following MDMA treatment. Administration of the NMDA antagonist MK-801 or the glutamate transporter type 1 (GLT-1) inducer ceftriaxone prevented both the MDMA-induced loss of GAD67-IR neurons and the increased vulnerability to kainic acid-induced seizures. The MDMA-induced increase in the extracellular concentration of glutamate in the hippocampus was significantly diminished in rats treated with ceftriaxone, thereby implicating a glutamatergic mechanism in the neuroprotective effects of ceftriaxone. In summary, the present findings support a role for increased extracellular glutamate and NMDA receptor activation in the MDMA-induced loss of hippocampal GAD67-IR neurons and the subsequent increased susceptibility to evoked seizures.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica/efeitos dos fármacos
Glutamato Descarboxilase/metabolismo
Alucinógenos/farmacologia
Hipocampo/citologia
N-Metil-3,4-Metilenodioxianfetamina/farmacologia
Neurônios/efeitos dos fármacos
Neurônios/enzimologia
[Mh] Termos MeSH secundário: Animais
Temperatura Corporal/efeitos dos fármacos
Modelos Animais de Doenças
Maleato de Dizocilpina/uso terapêutico
Agonistas de Aminoácidos Excitatórios/toxicidade
Ácido Glutâmico/metabolismo
Ácido Caínico/toxicidade
Masculino
Microdiálise
Ratos
Ratos Sprague-Dawley
Convulsões/induzido quimicamente
Convulsões/tratamento farmacológico
Convulsões/metabolismo
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Excitatory Amino Acid Agonists); 0 (Hallucinogens); 3KX376GY7L (Glutamic Acid); 6LR8C1B66Q (Dizocilpine Maleate); EC 4.1.1.15 (Glutamate Decarboxylase); EC 4.1.1.15 (glutamate decarboxylase 1); KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine); SIV03811UC (Kainic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  4 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28865330
[Au] Autor:Li X; Pi Z; Liu S; Wang W; Liu Z; Song F
[Ad] Endereço:National Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; The Base State Key Laboratory of the Ministry of Agriculture Agricultu
[Ti] Título:Online monitoring of astragaloside II metabolism using a homemade cultural device coupled with microdialysis and ultra-performance liquid chromatography-mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:141-148, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new system was described for the online monitoring of astragaloside II (AII) metabolism in intestinal microbial community. The system was based on a homemade cultural device coupled with microdialysis (MD) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Main improvements include a simplified anaerobic incubator enabling the experiment to be conducted in ambient atmosphere, continuous sampling, and decreased matrix effect. Importantly, our method distinctly decreases the interference of small molecules by adding 20mgml of 2-hydroxypropyl-ß-cyclodextrin (HP-ß-CD) to the perfusion fluid. Using the developed method, the metabolism of AII in intestinal bacteria was successfully investigated. Results were then compared with those obtained by conventional incubation and sampling method. We found that the integrated experimental system maintained the proper fermentation environment for bacteria and enabled high chromatography performance. With the advantages of auto-sampling, online detection, non-requirement of expensive fermenting equipment, and negligible matrix interference, the method can greatly contribute to the investigation of the dynamic biotransformation of astragalosides in complicated matrix-based biological samples.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Microdiálise/instrumentação
Saponinas/análise
Saponinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Desenho de Equipamento
Fezes/microbiologia
Microbioma Gastrointestinal/fisiologia
Modelos Lineares
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saponins); 0 (astragaloside II)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE


  5 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28542429
[Au] Autor:Amdisen C; Jespersen B; Møldrup U; Keller AK
[Ad] Endereço:Institute of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark.
[Ti] Título:The unsuitability of implantable Doppler probes for the early detection of renal vascular complications - a porcine model for prevention of renal transplant loss.
[So] Source:PLoS One;12(5):e0178301, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vascular occlusion is a rare, but serious complication after kidney transplantation often resulting in graft loss. We therefore aimed to develop an experimental porcine model for stepwise reduction of the renal venous blood flow and to compare an implantable Doppler probe and microdialysis for fast detection of vascular occlusion. METHODS: In 20 pigs, implantable Doppler probes were placed on the renal artery and vein and a microdialysis catheter was placed in the renal cortex. An arterial flowprobe served as gold standard. Following two-hour baseline measurements, the pigs were randomised to stepwise venous occlusion, complete venous occlusion, complete arterial occlusion or controls. RESULTS: All parameters were stable through baseline measurements. Glutamate and lactate measured by microdialysis increased significantly (p = 0.02 and p = 0.03 respectively) 30 minutes after a 2/3 (66%) reduction in renal blood flow. The implantable Doppler probe was not able to detect flow changes until there was total venous occlusion. Microdialysis detected changes in local metabolism after both arterial and venous occlusion; the implantable Doppler probe could only detect vascular occlusions on the vessel it was placed. CONCLUSIONS: We developed a new model for stepwise renal venous blood flow occlusion. Furthermore, the first comparison of the implantable Doppler probe and microdialysis for detection of renal vascular occlusions was made. The implantable Doppler probe could only detect flow changes after a complete occlusion, whereas microdialysis detected changes earlier, and could detect both arterial and venous occlusion. Based on these results, the implantable Doppler probe for early detection of vascular occlusions cannot be recommended.
[Mh] Termos MeSH primário: Transplante de Rim/efeitos adversos
Rim/irrigação sanguínea
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Feminino
Rim/diagnóstico por imagem
Microdiálise
Artéria Renal/diagnóstico por imagem
Circulação Renal
Veias Renais/diagnóstico por imagem
Suínos
Falha de Tratamento
Ultrassonografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178301


  6 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28521971
[Au] Autor:Yuan W; Wu JY; Zhao YZ; Li J; Li JB; Li ZH; Li CS
[Ad] Endereço:Department of Emergency, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China; Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, China.
[Ti] Título:Comparison of early sequential hypothermia and delayed hypothermia on neurological function after resuscitation in a swine model.
[So] Source:Am J Emerg Med;35(11):1645-1652, 2017 Nov.
[Is] ISSN:1532-8171
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We utilized a porcine cardiac arrest model to compare early sequential hypothermia (ESH) with delayed hypothermia (DH) and no hypothermia (NH) to investigate the different effects on cerebral function after resuscitation. METHODS: After return of spontaneous circulation (ROSC), resuscitated 24 pigs divided into three groups. The ESH group implemented early sequential hypothermia immediately, and the DH group implemented delayed hypothermia at 1 h after ROSC. The core temperature, hemodynamic parameters and oxygen metabolism were recorded. Cerebral metabolism variables and neurotransmitter in the extracellular fluid were collected through the microdialysis tubes. The bloods were analyzed for venous jugular bulb oxygen saturation, lactate and neuron specific nolase. The cerebral function was evaluated using the cerebral performance category and neurologic deficit score at 72h after ROSC and cerebral histology in the right posterior frontal lobe were collected. RESULTS: ESH reached the target temperature earlier and showed more favorable outcomes of neurological function than DH. Specifically, early sequential hypothermia reduced cerebral oxygen and energy consumption and decreased extracellular accumulation of neurotransmitters after resuscitation and protected the integrity of the BBB during reperfusion. CONCLUSIONS: Early sequential hypothermia could increase the protection of neurological function after resuscitation and produce better neurological outcomes. The institutional protocol number: 2010-D-013.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Reanimação Cardiopulmonar
Lobo Frontal/patologia
Parada Cardíaca/terapia
Hipotermia Induzida/métodos
[Mh] Termos MeSH secundário: Animais
Encéfalo/irrigação sanguínea
Encéfalo/patologia
Modelos Animais de Doenças
Dopamina/metabolismo
Glucose/metabolismo
Glicerol/metabolismo
Ácido Láctico/metabolismo
Masculino
Microdiálise
Neurotransmissores/metabolismo
Norepinefrina/metabolismo
Fosfopiruvato Hidratase/metabolismo
Sus scrofa
Suínos
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotransmitter Agents); 33X04XA5AT (Lactic Acid); EC 4.2.1.11 (Phosphopyruvate Hydratase); IY9XDZ35W2 (Glucose); PDC6A3C0OX (Glycerol); VTD58H1Z2X (Dopamine); X4W3ENH1CV (Norepinephrine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE


  7 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28521250
[Au] Autor:Kala A; Patel YT; Davis A; Stewart CF
[Ad] Endereço:Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN, United States.
[Ti] Título:Development and validation of LC-MS/MS methods for the measurement of ribociclib, a CDK4/6 inhibitor, in mouse plasma and Ringer's solution and its application to a cerebral microdialysis study.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1057:110-117, 2017 Jul 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:LC-MS/MS methods to measure ribociclib in mouse plasma and Ringer's solution were successfully developed and validated. Reverse phase chromatography was performed with gradient elution using C18 (100A, 50×4.6mm, 3µ) and C8-A (50×2.0mm, 5µ) columns for plasma and Ringer's samples, respectively. Mouse plasma samples were extracted using solid phase extraction method, whereas no extraction was required for the Ringer's solution samples. Analytes were detected using positive ion MRM mode. The precursor to product ions (Q1→Q3) selected for ribociclib and d6-ribociclib were (m/z) 435.2→252.1 and 441.2→252.1, respectively. The linear range of quantification of ribociclib was 62.5-10,000ng/ml for plasma method and 0.1-100ng/ml for Ringer's solution method. The results for the inter-day and intra-day accuracy and precision of quality control samples were within the acceptable range. The lower limit of quantitation (LLOQ) for plasma and Ringer's samples were 62.5ng/ml (S/N>30) and 0.1ng/ml (S/N>13), respectively, whereas the limit of detection (LOD) was 6.9ng/ml (S/N>7) and 0.05ng/ml (S/N>3), respectively. The developed methods were successfully applied to the analysis of ribociclib in mouse plasma and dialysate samples collected during a cerebral microdialysis study of ribociclib in a non-tumor bearing mouse.
[Mh] Termos MeSH primário: Aminopiridinas/sangue
Encéfalo/metabolismo
Cromatografia Líquida de Alta Pressão/métodos
Soluções Isotônicas/química
Purinas/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Aminopiridinas/administração & dosagem
Aminopiridinas/farmacocinética
Animais
Química Encefálica
Calibragem
Cromatografia de Fase Reversa
Líquido Extracelular/química
Feminino
Camundongos
Microdiálise
Purinas/administração & dosagem
Purinas/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Aminopyridines); 0 (Isotonic Solutions); 0 (Purines); 8026-10-6 (Ringer's solution); TK8ERE8P56 (ribociclib)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE


  8 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28479102
[Au] Autor:Papouin T; Dunphy JM; Tolman M; Dineley KT; Haydon PG
[Ad] Endereço:Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA.
[Ti] Título:Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness.
[So] Source:Neuron;94(4):840-854.e7, 2017 May 17.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The activation of the N-methyl D-aspartate receptor (NMDAR) is controlled by a glutamate-binding site and a distinct, independently regulated, co-agonist-binding site. In most brain regions, the NMDAR co-agonist is the astrocyte-derived gliotransmitter D-serine. We found that D-serine levels oscillate in mouse hippocampus as a function of wakefulness, in vitro and in vivo. This causes a full saturation of the NMDAR co-agonist site in the dark (active) phase that dissipates to sub-saturating levels during the light (sleep) phase, and influences learning performance throughout the day. We demonstrate that hippocampal astrocytes sense the wakefulness-dependent activity of septal cholinergic fibers through the α7-nicotinic acetylcholine receptor (α7nAChR), whose activation drives D-serine release. We conclude that astrocytes tune the gating of synaptic NMDARs to the vigilance state and demonstrate that this is directly relevant to schizophrenia, a disorder characterized by NMDAR and cholinergic hypofunctions. Indeed, bypassing cholinergic activity with a clinically tested α7nAChR agonist successfully enhances NMDAR activation. VIDEO ABSTRACT.
[Mh] Termos MeSH primário: Astrócitos/metabolismo
Potenciais Pós-Sinápticos Excitadores
Hipocampo/metabolismo
Receptores de N-Metil-D-Aspartato/metabolismo
Esquizofrenia/metabolismo
Sinapses/metabolismo
Vigília/genética
Receptor Nicotínico de Acetilcolina alfa7/genética
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Condicionamento (Psicologia)
Eletroencefalografia
Eletromiografia
Medo
Hipocampo/citologia
Imuno-Histoquímica
Aprendizagem
Memória
Camundongos
Camundongos Transgênicos
Microdiálise
Músculos do Pescoço
Agonistas Nicotínicos/farmacologia
Imagem Óptica
Optogenética
Quinuclidinas/farmacologia
Receptores de N-Metil-D-Aspartato/efeitos dos fármacos
Serina
Tiofenos/farmacologia
Vigília/efeitos dos fármacos
Receptor Nicotínico de Acetilcolina alfa7/agonistas
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (7-chloro-N-quinuclidin-3-yl-benzo(b)thiophene-2-carboxamide); 0 (Chrna7 protein, mouse); 0 (Nicotinic Agonists); 0 (Quinuclidines); 0 (Receptors, N-Methyl-D-Aspartate); 0 (Thiophenes); 0 (alpha7 Nicotinic Acetylcholine Receptor); 452VLY9402 (Serine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE


  9 / 10549 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28433865
[Au] Autor:Zandy SL; Doherty JM; Wibisono ND; Gonzales RA
[Ad] Endereço:Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA.
[Ti] Título:High sensitivity HPLC method for analysis of in vivo extracellular GABA using optimized fluorescence parameters for o-phthalaldehyde (OPA)/sulfite derivatives.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1055-1056:1-7, 2017 Jun 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Reversed-phase HPLC with derivatization using o-phthalaldehyde (OPA) and sulfite allows electrochemical detection of γ-aminobutyric acid (GABA) in microdialysis samples. However, OPA/sulfite derivatives have been reported to produce lower fluorescent yield than OPA derivatives using organic thiols as the nucleophile. To overcome this limitation we examined excitation and emission spectra, reaction time, pH, and concentration of reagents in the derivatization solution. Optimal detection parameters were determined as λ =220nm and λ =385nm for maximal fluorescence. The derivatization reaction occurred immediately and the product was stable up to 10min. A pH of 10.4 for the borate buffer used in the derivatization solution was significantly better than lower pH. Increasing the amount of sulfite combined with diluting the derivatization solution in borate buffer resulted in complete separation of the GABA peak from contaminants without any loss in signal. Controlling the temperature of the detector at 15°C significantly improved sensitivity with a detection limit of approximately 1nM. To validate this assay, we performed microdialysis in the dorsal striatum and ventral tegmental area (VTA) of adult Long Evans rats. GABA concentrations in dialysates were determined using external standards and standard additions, in order to further confirm interfering peaks were not present in biological samples. Within the dorsal striatum (n=4), basal GABA concentrations were 12.9±2.2 and 14.5±2.2nM (external and additions, respectively). Respective basal GABA concentrations in the VTA (n=3) were 4.6±1.1 and 5.1±0.6nM. Thus, we have developed a novel, sensitive fluorescence method to determine GABA in microdialysates using HPLC of an OPA/sulfite derivative.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Microdiálise/métodos
Ácido gama-Aminobutírico/análise
[Mh] Termos MeSH secundário: Animais
Corpo Estriado/química
Fluorescência
Limite de Detecção
Masculino
Ratos Long-Evans
Sulfitos/química
o-Ftalaldeído/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Sulfites); 56-12-2 (gamma-Aminobutyric Acid); 643-79-8 (o-Phthalaldehyde)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE


  10 / 10549 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28426993
[Au] Autor:Zheng L; Zhao XE; Zhu S; Tao Y; Ji W; Geng Y; Wang X; Chen G; You J
[Ad] Endereço:Shandong Provincial Key Laboratory of Life-Organic Analysis & Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, Shandong, PR China.
[Ti] Título:A new combined method of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in rat brain microdialysates by ultra high performance liquid chromatography tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1054:64-72, 2017 Jun 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this work, for the first time, a new hyphenated technique of stable isotope-labeling derivatization-ultrasound-assisted dispersive liquid-liquid microextraction has been developed for the simultaneous determination of monoamine neurotransmitters (MANTs) and their biosynthesis precursors and metabolites. The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry detection using multiple-reaction monitoring mode. A pair of mass spectrometry sensitizing reagents, d -10-methyl-acridone-2-sulfonyl chloride and d -10-methyl-acridone-2-sulfonyl chloride, as stable isotope probes was utilized to facilely label neurotransmitters, respectively. The heavy labeled MANTs standards were prepared and used as internal standards for quantification to minimize the matrix effects in mass spectrometry analysis. Low toxic bromobenzene (extractant) and acetonitrile (dispersant) were utilized in microextraction procedure. Under the optimized conditions, good linearity was observed with the limits of detection (S/N>3) and limits of quantification (S/N>10) in the range of 0.002-0.010 and 0.015-0.040nmol/L, respectively. Meanwhile, it also brought acceptable precision (4.2-8.8%, peak area RSDs %) and accuracy (recovery, 96.9-104.1%) results. This method was successfully applied to the simultaneous determination of monoamine neurotransmitters and their biosynthesis precursors and metabolites in rat brain microdialysates of Parkinson's disease and normal rats. This provided a new method for the neurotransmitters related studies in the future.
[Mh] Termos MeSH primário: Química Encefálica
Cromatografia Líquida de Alta Pressão/métodos
Microextração em Fase Líquida/métodos
Microdiálise/métodos
Neurotransmissores/análise
[Mh] Termos MeSH secundário: Animais
Encéfalo/metabolismo
Marcação por Isótopo/métodos
Masculino
Metabolômica/métodos
Neurotransmissores/metabolismo
Ratos
Ratos Sprague-Dawley
Sonicação/métodos
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotransmitter Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE



página 1 de 1055 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde