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Pesquisa : E05.196.401 [Categoria DeCS]
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[PMID]:27775531
[Au] Autor:Manocha P; Chandwani G; Das S
[Ti] Título:Dielectrophoretic Relay Assisted Molecular Communication for In-Sequence Molecule Delivery.
[So] Source:IEEE Trans Nanobioscience;15(7):781-791, 2016 10.
[Is] ISSN:1558-2639
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:With current research focus to interconnect the molecular communication environment with external environment, it is imperative to design external devices working on molecular communication schemes to be interfaced with in-vivo molecular network. Recently, efforts have been made to integrate molecular communication with Lab-on-chip (LOC); one of the techniques used in LOC for manipulation and transportation of molecules is Dielctrophoresis (DEP). We propose the use of DEP in molecular communication to maintain in-sequence delivery of molecules. DEP planar electrodes are modeled as relays used in telecommunications. We describe the theoretical system model and analyze the effect of introducing DEP relays in diffusive channel in terms of probability of in-sequence delivery of molecules. Information rate of DEP-based channel is analytically obtained for in-sequence delivery. The numerical results obtained show that the information rate for in-sequence delivery of molecules through diffusive channel increases by 26% if DEP relays are used in the channel. Though the system is sensitive to noise variance, incorporation of DEP relay results in a substantial improvement in the capacity of the channel.
[Mh] Termos MeSH primário: Computadores Moleculares
Eletroforese/métodos
Dispositivos Lab-On-A-Chip
[Mh] Termos MeSH secundário: Eletrodos
Análise de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1109/TNB.2016.2618904


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[PMID]:27775528
[Au] Autor:Hou J; Luo T; Ng KL; Leung AY; Liang R; Sun D
[Ti] Título:Characterization of Drug Effect on Leukemia Cells Through Single Cell Assay With Optical Tweezers and Dielectrophoresis.
[So] Source:IEEE Trans Nanobioscience;15(8):820-827, 2016 12.
[Is] ISSN:1558-2639
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the greatest challenges in acute myeloid leukemia (AML) treatment is preventing relapse. Leukemia cells can hide in bone marrow niche or vascular niche. Hence, many chemical drugs cannot kill these cells. To characterize migration and adhesion properties of leukemia cells in specific niches, CXCR4/SDF- 1α signal pathway has been widely used for investigation. AMD3100 is treated as one of the most common chemical drugs that can inhibit this signal. In the current study, we particularly investigate the effect of AMD3100 on the adhesion property of leukemia cells on stromal cells by using engineering tools, namely, optical tweezers (OT) and dielectrophoresis (DEP), to probe single cell property. AMD3100 not only inhibits the CXCR4/SDF- 1α signal pathway but also reduces gene expression of CXCR4 and VLA-4 on leukemia cells. The drug also softens leukemia cells. This work provides a new way to investigate cell behavior under drug treatment. The use of combined engineering tools will benefit drug discovery and assessment for leukemia treatment.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Eletroforese/métodos
Compostos Heterocíclicos/farmacologia
Leucemia Mieloide Aguda/metabolismo
Pinças Ópticas
Análise de Célula Única/métodos
[Mh] Termos MeSH secundário: Adesão Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Quimiocina CXCL12/análise
Quimiocina CXCL12/genética
Quimiocina CXCL12/metabolismo
Técnicas de Cocultura
Seres Humanos
Receptores CXCR4/análise
Receptores CXCR4/genética
Receptores CXCR4/metabolismo
Transdução de Sinais/efeitos dos fármacos
Células Estromais/citologia
Células Estromais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (CXCR4 protein, human); 0 (Chemokine CXCL12); 0 (Heterocyclic Compounds); 0 (Receptors, CXCR4); 155148-31-5 (JM 3100)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1109/TNB.2016.2616160


  3 / 34352 MEDLINE  
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[PMID]:28743396
[Au] Autor:Finetti C; Sola L; Elliott J; Chiari M
[Ad] Endereço:National Research Council of Italy, Institute of Chemistry of Molecular Recognition, Via Mario Bianco 9 20131 Milan, Italy.
[Ti] Título:Synthesis of hydrogel via click chemistry for DNA electrophoresis.
[So] Source:J Chromatogr A;1513:226-234, 2017 Sep 01.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This work introduces a novel sieving gel for DNA electrophoresis using a classical click chemistry reaction, the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), to cross-link functional polymer chains. The efficiency of this reaction provides, under mild conditions, hydrogels with near-ideal network connectivity and improved physical properties. Hydrogel formation via click chemistry condensation of functional polymers does not involve the use of toxic monomers and UV initiation. The performance of the new hydrogel in the separation of double stranded DNA fragments was evaluated in the 2200 TapeStation system, an analytical platform, recently introduced by Agilent that combines the advantages of CE in terms of miniaturization and automation with the simplicity of use of slab gel electrophoresis. The click gel enables addition of florescent dyes prior to electrophoresis with considerable improvement of resolution and separation efficiency over conventional cross-linked polyacrylamide gels.
[Mh] Termos MeSH primário: Química Click/métodos
DNA/análise
Eletroforese/métodos
Hidrogel de Polietilenoglicol-Dimetacrilato/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Alquinos/química
Catálise
Polietilenoglicóis/química
Sefarose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Alkynes); 0 (polyacrylamide gels); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); 30IQX730WE (Polyethylene Glycols); 9007-49-2 (DNA); 9012-36-6 (Sepharose)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:28467599
[Au] Autor:Lapizco-Encinas BH
[Ti] Título:Editorial-Dielectrophoresis 2017.
[So] Source:Electrophoresis;38(11):1405-1406, 2017 06.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Eletroforese
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201700169


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[PMID]:28467596
[Au] Autor:Gilmore J; Islam M; Duncan J; Natu R; Martinez-Duarte R
[Ad] Endereço:Multiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC, USA.
[Ti] Título:Assessing the importance of the root mean square (RMS) value of different waveforms to determine the strength of a dielectrophoresis trapping force.
[So] Source:Electrophoresis;38(20):2561-2564, 2017 10.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Eletroforese
Modelos Teóricos
[Mh] Termos MeSH secundário: Eletricidade
Microesferas
Poliestirenos
Processamento de Sinais Assistido por Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polystyrenes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201600551


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[PMID]:28981578
[Au] Autor:Fry SC
[Ad] Endereço:The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Max Born Crescent, Edinburgh EH9 3BF, UK.
[Ti] Título:Potassium, not lepidimoide, is the principal 'allelochemical' of cress-seed exudate that promotes amaranth hypocotyl elongation.
[So] Source:Ann Bot;120(4):511-520, 2017 Oct 17.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background and Aims: Imbibed cress ( Lepidium sativum L.) seeds exude 'allelochemicals' that promote excessive hypocotyl elongation and inhibit root growth in neighbouring competitors, e.g. amaranth ( Amaranthus caudatus L.) seedlings. The major hypocotyl promoter has recently been shown not to be the previously suggested acidic disaccharide, lepidimoic acid (LMA), a fragment of the pectic polysaccharide domain rhamnogalacturonan-I. The nature of the hypocotyl promoter has now been re-assessed. Methods: Low-molecular weight cress-seed exudate (LCSE) was fractionated by high-voltage electrophoresis, and components with different charge:mass ratios were tested for effects on dark-grown amaranth seedlings. Further samples of LCSE were size-fractionated by gel permeation chromatography, and active fractions were analysed electrophoretically. Key Results: The LCSE strongly promoted amaranth hypocotyl elongation. The active principle was hydrophilic and, unlike LMA, stable to hot acid. After electrophoresis at pH 6·5, the only fractions that strongly promoted hypocotyl elongation were those with a very high positive charge:mass ratio, migrating towards the cathode 3-4 times faster than glucosamine. Among numerous naturally occurring cations tested, the only one with such a high mobility was potassium. K + was present in LCSE at approx. 4 m m , and pure KCl (1-10 m m ) strongly promoted amaranth hypocotyl elongation. No other cation tested (including Na + , spermidine and putrescine) had this effect. The peak of bioactivity from a gel permeation chromatography column exactly coincided with the peak of K + . Conclusions: The major 'allelopathic' substance present in cress-seed exudate that stimulates hypocotyl elongation in neighbouring seedlings is the inorganic cation, K + , not the oligosaccharin LMA.
[Mh] Termos MeSH primário: Amaranthus/crescimento & desenvolvimento
Dissacarídeos/fisiologia
Exsudatos e Transudatos/fisiologia
Hipocótilo/crescimento & desenvolvimento
Lepidium sativum/fisiologia
Potássio/fisiologia
Sementes/metabolismo
[Mh] Termos MeSH secundário: Amaranthus/metabolismo
Cromatografia em Gel
Eletroforese/métodos
Exsudatos e Transudatos/química
Hipocótilo/metabolismo
Lepidium sativum/metabolismo
Sementes/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disaccharides); 145039-76-5 (lepidimoide); RWP5GA015D (Potassium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcx081


  7 / 34352 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:28902924
[Au] Autor:Mohammadabadi MR; Jafari AHD; Bordbar F
[Ad] Endereço:Animal Science Department, Shahid Bahonar University of Kerman, Kerman, Iran.
[Ti] Título:Molecular analysis of CIB4 gene and protein in Kermani sheep.
[So] Source:Braz J Med Biol Res;50(11):e6177, 2017 Sep 12.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Different tissues were collected from the Kermani sheep and real time PCR was performed. The PCR products were sequenced, comparative analyses of the nucleotide sequences were performed, a phylogenetic tree was constructed, and different characteristics of CIB4 proteins were predicted. Real time PCR results showed that the CIB4 gene is expressed only in testis of Kermani sheep. The cDNA nucleotide sequence was identical with small tail Han sheep, cattle, goat, camel, horse, dog, mouse and human, respectively 100, 99, 99, 98, 98, 96, 96, and 96%. Hence, it can be suggested that the CIB4 gene plays a role in male fertility. Based on the phylogenetic analysis, sheep CIB4 gene has a close relationship with goat and cattle first, and then with camel and whale. Although we demonstrated that CIB4 is a testis-specific gene, expressed only in the testis and it interacts with other proteins, the mechanisms by which CIB4 expression is regulated need to be elucidated.
[Mh] Termos MeSH primário: Proteínas de Ligação ao Cálcio/análise
Proteínas de Ligação ao Cálcio/genética
Ovinos/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Eletroforese/veterinária
Feminino
Masculino
Filogenia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Valores de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE


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[PMID]:28833103
[Au] Autor:Wakita S; Kobayashi S; Kimura M; Kashimura A; Honda S; Sakaguchi M; Sugahara Y; Kamaya M; Matoska V; Bauer PO; Oyama F
[Ad] Endereço:Department of Chemistry and Life Science, Kogakuin University, Hachioji, Tokyo, Japan.
[Ti] Título:Mouse acidic mammalian chitinase exhibits transglycosylation activity at somatic tissue pH.
[So] Source:FEBS Lett;591(20):3310-3318, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mouse acidic mammalian chitinase (AMCase) degrades chitin with highest efficiency at pH 2.0 and is active up to pH 8.0. Here, we report that mouse AMCase also exhibits transglycosylation activity under neutral conditions. We incubated natural and artificial chitin substrates with mouse AMCase at pH 2.0 or 7.0 and analyzed the resulting oligomers using an improved method of fluorescence-assisted carbohydrate electrophoresis. Mouse AMCase produces primarily dimers of N-acetyl-d-glucosamine [(GlcNAc) ] under both pH conditions while generating transglycosylated (GlcNAc) primarily at pH 7.0 and at lower levels at pH 2.0. These results indicate that mouse AMCase catalyzes hydrolysis as well as transglycosylation and suggest that this enzyme can play a novel role under physiological conditions in peripheral tissues, such as the lungs.
[Mh] Termos MeSH primário: Acetilglucosamina/metabolismo
Quitina/metabolismo
Quitinases/metabolismo
[Mh] Termos MeSH secundário: Animais
Quitinases/genética
Clonagem Molecular
Dimerização
Eletroforese/métodos
Escherichia coli/genética
Escherichia coli/metabolismo
Fluorescência
Expressão Gênica
Glicosilação
Concentração de Íons de Hidrogênio
Hidrólise
Cinética
Pulmão/enzimologia
Camundongos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Recombinant Proteins); 1398-61-4 (Chitin); EC 3.2.1.14 (AMCase, mouse); EC 3.2.1.14 (Chitinases); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12798


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[PMID]:28806755
[Au] Autor:Flierl U; Tongers J; Berliner D; Sieweke JT; Zauner F; Wingert C; Riehle C; Bauersachs J; Schäfer A
[Ad] Endereço:Hannover Medical School, Department of Cardiology and Angiology, Cardiac Arrest Center and Advanced Heart Failure Unit, Hannover, Germany.
[Ti] Título:Acquired von Willebrand syndrome in cardiogenic shock patients on mechanical circulatory microaxial pump support.
[So] Source:PLoS One;12(8):e0183193, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Early use of mechanical circulatory support, e.g. veno-arterial extracorporeal membrane oxygenation (ECMO) or left ventricular unloading by microaxial pump in refractory cardiogenic shock is recommended in current guidelines. Development of acquired von Willebrand Syndrome (AVWS) in patients with left ventricular assist devices (LVADs) and ECMO has been reported. There is an increasing number of patients treated with the Impella® CP microaxial pump for left ventricular unloading. However, the prevalence of AVWS in these high risk patients is unknown and needs to be determined. We therefore screened 21 patients (68 ± 11years) treated with Impella® (17 for cardiogenic shock, 4 for protected PCI) for the presence of AVWS by determining von Willebrand factor multimers, VWF collagen binding capacity and VWF antigen. During the time course of Impella® support, 20/21 patients (95%) developed AVWS (mean duration of support: 135 ± 114 hours, mean time from device implantation to first diagnosis of AVWS: 10.6 ± 10.8 hours). Our data indicate that AVWS is a common phenomenon during left ventricular unloading via microaxial pump support. Thus, AVWS has to be considered as contributing factor for potential bleeding complications in this high risk patient population, especially in the context of dual antiplatelet therapy.
[Mh] Termos MeSH primário: Coração Auxiliar/efeitos adversos
Choque Cardiogênico/complicações
Doenças de von Willebrand/etiologia
[Mh] Termos MeSH secundário: Idoso
Antígenos/metabolismo
Colágeno/metabolismo
Eletroforese
Feminino
Seres Humanos
Masculino
Ligação Proteica
Síndrome
Doenças de von Willebrand/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Antigens); 9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183193


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[PMID]:28733325
[Au] Autor:Vidmar R; Vizovisek M; Turk D; Turk B; Fonovic M
[Ad] Endereço:Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
[Ti] Título:Protease cleavage site fingerprinting by label-free in-gel degradomics reveals pH-dependent specificity switch of legumain.
[So] Source:EMBO J;36(16):2455-2465, 2017 Aug 15.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Determination of protease specificity is of crucial importance for understanding protease function. We have developed the first gel-based label-free proteomic approach (DIPPS-direct in-gel profiling of protease specificity) that enables quick and reliable determination of protease cleavage specificities under large variety of experimental conditions. The methodology is based on in-gel digestion of the gel-separated proteome with the studied protease, enrichment of cleaved peptides by gel extraction, and subsequent mass spectrometry analysis combined with a length-limited unspecific database search. We applied the methodology to profile ten proteases ranging from highly specific (trypsin, endoproteinase GluC, caspase-7, and legumain) to broadly specific (matrix-metalloproteinase-3, thermolysin, and cathepsins K, L, S, and V). Using DIPPS, we were able to perform specificity profiling of thermolysin at its optimal temperature of 75°C, which confirmed the applicability of the method to extreme experimental conditions. Moreover, DIPPS enabled the first global specificity profiling of legumain at pH as low as 4.0, which revealed a pH-dependent change in the specificity of this protease, further supporting its broad applicability.
[Mh] Termos MeSH primário: Cisteína Endopeptidases/metabolismo
Proteômica/métodos
[Mh] Termos MeSH secundário: Eletroforese
Concentração de Íons de Hidrogênio
Espectrometria de Massas
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.22.- (Cysteine Endopeptidases); EC 3.4.22.34 (asparaginylendopeptidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170723
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201796750



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