Base de dados : MEDLINE
Pesquisa : E05.196.401.190.500 [Categoria DeCS]
Referências encontradas : 1222 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 123 ir para página                         

  1 / 1222 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28410480
[Au] Autor:Li S; Yang T; Zhao J; Huang Y; Zhao S
[Ad] Endereço:State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, 541004, China.
[Ti] Título:Chemiluminescence noncompetitive immunoassay based on microchip electrophoresis for the determination of ß-subunit of human chorionic gonadotropin.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1053:42-47, 2017 May 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this work, a microchip-electrophoresis chemiluminescence (MCE-CL)-based immunoassay method was established for the determination of ß-subunit of human chorionic gonadotropin (ß-HCG). This approach uses MCE-CL assay as a platform. First, the ß-HCG antigen (Ag) binds to excess horseradish peroxidase (HRP)-labeled anti-ß-HCG antibodies (Ab*) to form an immune complex (Ag-Ab ). Subsequently, the obtained Ag-Ab complex and unreacted Ab were separated by MCE, and detected by CL. The CL intensity (peak high) of Ag-Ab was used to estimate ß-HCG concentration. The calibration curve between the peak high and ß-HCG concentration showed a good linearity in the range of 0.6-60mIU/mL. Based on a signal/noise ratio (S/N) of 3, the detection limit for ß-HCG was estimated to be 0.36mIU/mL. The present method was successfully applied for the detection of ß-HCG in human serum, and the serum content of ß-HCG from three healthy subjects was found be in the range of 9.5-15.7mIU/mL, while that from three ovarian cancer patients was found be in the range of 160.9-210.4mIU/mL. These results suggest that cancer patients have higher contents of ß-HCG than healthy individuals do, indicating that this method can be applied for assisting diagnosis of ovarian cancer in clinical application.
[Mh] Termos MeSH primário: Gonadotropina Coriônica Humana Subunidade beta/sangue
Eletroforese em Microchip/métodos
[Mh] Termos MeSH secundário: Anticorpos Monoclonais/química
Biomarcadores Tumorais/sangue
Eletroforese em Microchip/instrumentação
Desenho de Equipamento
Seres Humanos
Imunoensaio/instrumentação
Imunoensaio/métodos
Limite de Detecção
Medições Luminescentes/instrumentação
Medições Luminescentes/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Biomarkers, Tumor); 0 (Chorionic Gonadotropin, beta Subunit, Human)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE


  2 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28396087
[Au] Autor:Moreira RC; Lopes MS; Medeiros Junior I; Coltro WKT
[Ad] Endereço:Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
[Ti] Título:High performance separation of quaternary amines using microchip non-aqueous electrophoresis coupled with contactless conductivity detection.
[So] Source:J Chromatogr A;1499:190-195, 2017 May 26.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study describes the development of an analytical methodology for the separation of quaternary amines using non-aqueous microchip electrophoresis (NAME) coupled with capacitively coupled contactless conductivity detection (C D). All experiments were performed using a commercial microchip electrophoresis system consisting of a C D detector, a high-voltage sequencer and a microfluidic platform to assemble a glass microchip with integrated sensing electrodes. The detection parameters were optimized and the best response was reached applying a 700-kHz sinusoidal wave with 14V excitation voltage. The running electrolyte composition was optimized aiming to achieve the best analytical performance. The mixture containing methanol and acetonitrile at the proportion of 90:10 (v:v) as well as sodium deoxycholate provided separations of ten quaternary amines with high efficiency and baseline resolution. The separation efficiencies ranged from 8.7×10 to 3.0×10 plates/m. The proposed methodology provided linear response in the concentration range between 50 and 1000µmol/L and limits of detection between 2 and 27µmol/L. The analytical feasibility of the proposed methodology was tested in the determination of quaternary amines in corrosion inhibitor samples often used for coating oil pipelines. Five quaternary amines (dodecyltrimethylammonium chloride, tetradecyltrimetylammonium bromide, cetyltrimethylammonium bromide, tetraoctylammonium bromide and tetradodecylammonium bromide) were successfully detected at concentration levels from 0.07 to 6.45mol/L. The accuracy of the developed methodology was investigated and the achieved recovery values varied from 85 to 122%. Based on the reported data, NAME-C D devices exhibited great potential to provide high performance separations of hydrophobic compounds. The developed methodology can be useful for the analysis of species that usually present strong adsorption on the channel inner walls.
[Mh] Termos MeSH primário: Aminas/química
Eletroforese em Microchip/métodos
[Mh] Termos MeSH secundário: Aminas/isolamento & purificação
Condutividade Elétrica
Eletrodos
Eletroforese em Microchip/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE


  3 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28347516
[Au] Autor:Tähkä SM; Bonabi A; Jokinen VP; Sikanen TM
[Ad] Endereço:Faculty of Pharmacy, Drug Research Programme, Viikinkaari 5E, FI-00014, University of Helsinki, Helsinki, Finland. Electronic address: sari.tahka@helsinki.fi.
[Ti] Título:Aqueous and non-aqueous microchip electrophoresis with on-chip electrospray ionization mass spectrometry on replica-molded thiol-ene microfluidic devices.
[So] Source:J Chromatogr A;1496:150-156, 2017 May 05.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This work describes aqueous and non-aqueous capillary electrophoresis on thiol-ene-based microfluidic separation devices that feature fully integrated and sharp electrospray ionization (ESI) emitters. The chip fabrication is based on simple and low-cost replica-molding of thiol-ene polymers under standard laboratory conditions. The mechanical rigidity and the stability of the materials against organic solvents, acids and bases could be tuned by adjusting the respective stoichiometric ratio of the thiol and allyl ("ene") monomers, which allowed us to carry out electrophoresis separation in both aqueous and non-aqueous (methanol- and ethanol-based) background electrolytes. The stability of the ESI signal was generally ≤10% RSD for all emitters. The respective migration time repeatabilities in aqueous and non-aqueous background electrolytes were below 3 and 14% RSD (n=4-6, with internal standard). The analytical performance of the developed thiol-ene microdevices was shown in mass spectrometry (MS) based analysis of peptides, proteins, and small molecules. The theoretical plate numbers were the highest (1.2-2.4×10 m ) in ethanol-based background electrolytes. The ionization efficiency also increased under non-aqueous conditions compared to aqueous background electrolytes. The results show that replica-molding of thiol-enes is a feasible approach for producing ESI microdevices that perform in a stable manner in both aqueous and non-aqueous electrophoresis.
[Mh] Termos MeSH primário: Eletroforese em Microchip
Técnicas Analíticas Microfluídicas
Peptídeos/análise
Proteínas/análise
Espectrometria de Massas por Ionização por Electrospray/métodos
Compostos de Sulfidrila/química
[Mh] Termos MeSH secundário: Solventes/química
Espectrometria de Massas por Ionização por Electrospray/instrumentação
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptides); 0 (Proteins); 0 (Solvents); 0 (Sulfhydryl Compounds); 059QF0KO0R (Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE


  4 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28342274
[Au] Autor:Douglas TA; Cemazar J; Balani N; Sweeney DC; Schmelz EM; Davalos RV
[Ad] Endereço:Bioelectromechanical Systems Laboratory, Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Blacksburg, VA, USA.
[Ti] Título:A feasibility study for enrichment of highly aggressive cancer subpopulations by their biophysical properties via dielectrophoresis enhanced with synergistic fluid flow.
[So] Source:Electrophoresis;38(11):1507-1514, 2017 06.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A common problem with cancer treatment is the development of treatment resistance and tumor recurrence that result from treatments that kill most tumor cells yet leave behind aggressive cells to repopulate. Presented here is a microfluidic device that can be used to isolate tumor subpopulations to optimize treatment selection. Dielectrophoresis (DEP) is a phenomenon where particles are polarized by an electric field and move along the electric field gradient. Different cell subpopulations have different DEP responses depending on their bioelectrical phenotype, which, we hypothesize, correlate with aggressiveness. We have designed a microfluidic device in which a region containing posts locally distorts the electric field created by an AC voltage and forces cells toward the posts through DEP. This force is balanced with a simultaneous drag force from fluid motion that pulls cells away from the posts. We have shown that by adjusting the drag force, cells with aggressive phenotypes are influenced more by the DEP force and trap on posts while others flow through the chip unaffected. Utilizing single-cell trapping via cell-sized posts coupled with a drag-DEP force balance, we show that separation of similar cell subpopulations may be achieved, a result that was previously impossible with DEP alone. Separated subpopulations maintain high viability downstream, and remain in a native state, without fluorescent labeling. These cells can then be cultured to help select a therapy that kills aggressive subpopulations equally or better than the bulk of the tumor, mitigating resistance and recurrence.
[Mh] Termos MeSH primário: Separação Celular
Eletroforese em Microchip/instrumentação
Eletroforese em Microchip/métodos
Dispositivos Lab-On-A-Chip
Neoplasias/patologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Separação Celular/instrumentação
Separação Celular/métodos
Simulação por Computador
Desenho de Equipamento/instrumentação
Desenho de Equipamento/métodos
Estudos de Viabilidade
Feminino
Seres Humanos
Fenômenos Mecânicos
Camundongos
Camundongos Endogâmicos C57BL
Microeletrodos
Modelos Teóricos
Movimento (Física)
Neoplasias Ovarianas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170326
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201600530


  5 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28277175
[Au] Autor:Xiao MW; Bai XL; Xu PL; Zhao Y; Yang L; Liu YM; Liao X
[Ad] Endereço:a Chengdu Institute of Biology , Chinese Academy of Sciences , Chengdu , China.
[Ti] Título:Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(5):760-765, 2017 May.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sensitive detection of gizzerosine, a causative agent for deadly gizzard erosion in chicken feeds, is very important to the poultry industry. In this work, a new method was developed based on microchip capillary electrophoresis (MCE) with laser-induced fluorescence (LIF) detection for rapid analysis of gizzerosine, a biogenic amine in fish meals. The MCE separation was performed on a glass microchip using sodium dodecyl sulfate (SDS) as dynamic coating modifier. Separation conditions, including running buffer pH and concentration, SDS concentration, and the separation voltage were investigated to achieve fast and sensitive quantification of gizzerosine. The assay proposed was very quick and could be completed within 65 s. A linear calibration curve was obtained in the range from 0.04 to 1.8 µg ml gizzerosine. The detection limit was 0.025 µg ml (0.025 mg kg ), which was far more sensitive than those previously reported. Gizzerosine was well separated from other endogenous components in fish meal samples. Recovery of gizzerosine from this sample matrix (n = 3) was determined to be 97.2-102.8%. The results from analysing fish meal samples indicated that the present MCE-LIF method might hold the potential for rapid detection of gizzerosine in poultry feeds.
[Mh] Termos MeSH primário: Ração Animal/análise
Peixes
Fluorescência
Imidazóis/análise
Lasers
[Mh] Termos MeSH secundário: Animais
Eletroforese em Microchip
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imidazoles); 89238-78-8 (gizzerosine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2017.1292053


  6 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28272749
[Au] Autor:Sonker M; Knob R; Sahore V; Woolley AT
[Ad] Endereço:Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
[Ti] Título:Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers.
[So] Source:Electrophoresis;38(13-14):1743-1754, 2017 07.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Integration in microfluidics is important for achieving automation. Sample preconcentration integrated with separation in a microfluidic setup can have a substantial impact on rapid analysis of low-abundance disease biomarkers. Here, we have developed a microfluidic device that uses pH-mediated solid-phase extraction (SPE) for the enrichment and elution of preterm birth (PTB) biomarkers. Furthermore, this SPE module was integrated with microchip electrophoresis for combined enrichment and separation of multiple analytes, including a PTB peptide biomarker (P1). A reversed-phase octyl methacrylate monolith was polymerized as the SPE medium in polyethylene glycol diacrylate modified cyclic olefin copolymer microfluidic channels. Eluent for pH-mediated SPE of PTB biomarkers on the monolith was optimized using different pH values and ionic concentrations. Nearly 50-fold enrichment was observed in single channel SPE devices for a low nanomolar solution of P1, with great elution time reproducibility (<7% RSD). The monolith binding capacity was determined to be 400 pg (0.2 pmol). A mixture of a model peptide (FA) and a PTB biomarker (P1) was extracted, eluted, injected, and then separated by microchip electrophoresis in our integrated device with ∼15-fold enrichment. This device shows important progress towards an integrated electrokinetically operated platform for preconcentration and separation of biomarkers.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Eletroforese em Microchip/instrumentação
Nascimento Prematuro
Extração em Fase Sólida/instrumentação
[Mh] Termos MeSH secundário: Eletroforese em Microchip/métodos
Desenho de Equipamento
Feminino
Seres Humanos
Polietilenoglicóis/química
Gravidez
Nascimento Prematuro/sangue
Nascimento Prematuro/diagnóstico
Reprodutibilidade dos Testes
Extração em Fase Sólida/métodos
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Biomarkers); 0 (poly(ethylene glycol)diacrylate); 30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201700054


  7 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28256738
[Au] Autor:Mansoorifar A; Koklu A; Sabuncu AC; Beskok A
[Ad] Endereço:Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA.
[Ti] Título:Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy.
[So] Source:Electrophoresis;38(11):1466-1474, 2017 06.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dielectric spectroscopy (DS) is a noninvasive, label-free, fast, and promising technique for measuring dielectric properties of biological cells in real time. We demonstrate a microchip that consists of electro-activated microwell arrays for positive dielectrophoresis assisted cell capture, DS measurements, and negative dielectrophoresis driven cell unloading; thus, providing a high-throughput cell analysis platform. To the best of our knowledge, this is the first microfluidic chip that combines electro-activated microwells and DS to analyze biological cells. Device performance is tested using Saccharomyces cerevisiae (yeast) cells. DEP response of yeast cells is determined by measuring their Clausius-Mossotti factor using biophysical models in parallel plate microelectrode geometry. This information is used to determine the excitation frequency to load and unload wells. Effect of yeast cells on the measured impedance spectrum was examined both experimentally and numerically. Good match between the numerical and experimental results establishes the potential use of the microchip device for extracting subcellular properties of biological cells in a rapid and nonexpensive manner.
[Mh] Termos MeSH primário: Espectroscopia Dielétrica/métodos
Eletroforese em Microchip
Dispositivos Lab-On-A-Chip
Microfluídica/instrumentação
Saccharomyces cerevisiae
[Mh] Termos MeSH secundário: Simulação por Computador
Eletroforese em Microchip/instrumentação
Eletroforese em Microchip/métodos
Desenho de Equipamento
Ouro
Microeletrodos
Modelos Teóricos
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
7440-57-5 (Gold)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201700020


  8 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28213894
[Au] Autor:Liu W; Ren Y; Tao Y; Li Y; Chen X
[Ad] Endereço:School of Electronics and Control Engineering, Chang'an University, Xi'an, Shaanxi, P. R. China.
[Ti] Título:Controllable rotating behavior of individual dielectric microrod in a rotating electric field.
[So] Source:Electrophoresis;38(11):1427-1433, 2017 06.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We report herein controllable rotating behavior of an individual dielectric microrod driven by a background rotating electric field. By disposing or removing structured floating microelectrode, the rigid rod suspended in electrolyte solution accordingly exhibits cofield or antifield rotating motion. In the absence of the ideally polarizable metal surface, the dielectric rod rotates opposite to propagation of electric field, with the measured rotating rate much larger than predicted by Maxwell-Wager interfacial polarization theory incorporating surface conduction of fixed bond charge. Surprisingly, with floating electrode embedded, a novel kind of cofield rotation mode occurs in the presence of induced double-layer polarization, due to the action of hydrodynamic torque from rotating induced-charge electroosmosis. This method of achieving switchable spin modes of dielectric particles would direct implications in constructing flexible electrokinetic framework for analyzing 3D profile of on-chip biomicrofluidic samples.
[Mh] Termos MeSH primário: Eletroforese
Microfluídica/métodos
Modelos Teóricos
[Mh] Termos MeSH secundário: Simulação por Computador
Eletricidade
Eletro-Osmose
Eletroforese/instrumentação
Eletroforese/métodos
Eletroforese em Microchip
Desenho de Equipamento
Microeletrodos
Técnicas Analíticas Microfluídicas
Rotação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201600574


  9 / 1222 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28187910
[Au] Autor:Tellez-Gabriel M; Charrier C; Brounais-Le Royer B; Mullard M; Brown HK; Verrecchia F; Heymann D
[Ad] Endereço:INSERM, UMR 957, Equipe LIGUE Nationale Contre le Cancer 2012, Nantes 44035, France; Université de Nantes, Nantes atlantique universités, Pathophysiology of Bone Resorption and Therapy of Primary Bone Tumours, Nantes, France; Laboratotio Hematologia Oncologica y de Transplantes, Institut Investigaci
[Ti] Título:Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip.
[So] Source:Eur J Cell Biol;96(2):110-118, 2017 Mar.
[Is] ISSN:1618-1298
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations. The present manuscript describes the setting up of a dielectrophoresis (DEP)-based lab-on-a-chip platform for the real-time study of Gap Junctional Intercellular Communication between osteosarcoma cells and the main cells accessible to their microenvironment. We conclude that using the DEParray technology for the GJIC assessment has several advantages comparing to current techniques. This methodology is less harmful for cells integrity; cells can be recovered after interaction to make further molecular analysis; it is possible to study GJIC in real time; we can promote cell interactions using up to five different populations. The setting up of this new methodology overcomes several difficulties to perform experiments for solving questions about GJIC process that we are not able to do with current technics.
[Mh] Termos MeSH primário: Comunicação Celular/fisiologia
Eletroforese em Microchip/instrumentação
Eletroforese em Microchip/métodos
Junções Comunicantes/fisiologia
[Mh] Termos MeSH secundário: Neoplasias Ósseas/patologia
Diferenciação Celular/fisiologia
Linhagem Celular Tumoral
Seres Humanos
Células Mesenquimais Estromais/citologia
Osteoblastos/citologia
Osteossarcoma/patologia
Imagem com Lapso de Tempo/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


  10 / 1222 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28112416
[Au] Autor:Allen DJ; Accolla RP; Williams SJ
[Ad] Endereço:Department of Mechanical Engineering, University of Louisville, Louisville, Kentucky, USA.
[Ti] Título:Isomotive dielectrophoresis for parallel analysis of individual particles.
[So] Source:Electrophoresis;38(11):1441-1449, 2017 06.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Two dielectrophoresis systems are introduced where the induced dielectrophoretic force is constant throughout the experimental region, resulting in uniform (isomotive) microparticle translation. Isomotive dielectrophoresis (isoDEP) is accomplished through a unique geometry where the gradient of the field-squared (∇Erms2) is constant, a characteristic that is otherwise highly nonuniform in traditional DEP platforms. The governing isoDEP equations were derived herein and applied to two different isoDEP prototypes: (i) one fabricated from deep reactive ion etching (DRIE) of a conductive silicon wafer (1-10 Ω-cm) whose patterned features served as electrodes and microchannel sidewalls simultaneously; (ii) a second where the electric field is applied lengthwise through a PDMS microchannel whose geometry follows a specific curvature. Both positive and negative dielectrophoresis was demonstrated with the isoDEP devices using silver-coated hollow glass spheres and polystyrene particles, respectively. Particle tracking was used to compare particle trajectory with the expected dielectrophoretic response; further, particle velocity was used to measure the Clausius-Mossotti factor of individual polystyrene particles (18-24.9 µm) in both devices with a value of -0.40 ± 0.063 (n = 110) and -0.48 ± 0.055 (n = 18) for the DRIE and PDMS isoDEP platforms, respectively. The isoDEP platform is capable of analyzing multiple particles simultaneously, providing greater throughput than traditional electrorotation platforms.
[Mh] Termos MeSH primário: Simulação por Computador
Eletroforese em Microchip
Desenho de Equipamento
[Mh] Termos MeSH secundário: Eletro-Osmose
Eletroforese em Microchip/instrumentação
Eletroforese em Microchip/métodos
Desenho de Equipamento/instrumentação
Desenho de Equipamento/métodos
Microeletrodos
Modelos Teóricos
Tamanho da Partícula
Poliestirenos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Polystyrenes)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201600517



página 1 de 123 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde