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[PMID]:28877216
[Au] Autor:Varadarajan B; Vogt A; Hartwich V; Vasireddy R; Consiglio J; Hugi-Mayr B; Eberle B
[Ad] Endereço:Department of Anesthesiology and Pain Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
[Ti] Título:An in vitro lung model to assess true shunt fraction by multiple inert gas elimination.
[So] Source:PLoS One;12(9):e0184212, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Multiple Inert Gas Elimination Technique, based on Micropore Membrane Inlet Mass Spectrometry, (MMIMS-MIGET) has been designed as a rapid and direct method to assess the full range of ventilation-to-perfusion (V/Q) ratios. MMIMS-MIGET distributions have not been assessed in an experimental setup with predefined V/Q-distributions. We aimed (I) to construct a novel in vitro lung model (IVLM) for the simulation of predefined V/Q distributions with five gas exchange compartments and (II) to correlate shunt fractions derived from MMIMS-MIGET with preset reference shunt values of the IVLM. Five hollow-fiber membrane oxygenators switched in parallel within a closed extracorporeal oxygenation circuit were ventilated with sweep gas (V) and perfused with human red cell suspension or saline (Q). Inert gas solution was infused into the perfusion circuit of the gas exchange assembly. Sweep gas flow (V) was kept constant and reference shunt fractions (IVLM-S) were established by bypassing one or more oxygenators with perfusate flow (Q). The derived shunt fractions (MM-S) were determined using MIGET by MMIMS from the retention data. Shunt derived by MMIMS-MIGET correlated well with preset reference shunt fractions. The in vitro lung model is a convenient system for the setup of predefined true shunt fractions in validation of MMIMS-MIGET.
[Mh] Termos MeSH primário: Pulmão/fisiologia
Troca Gasosa Pulmonar/fisiologia
Relação Ventilação-Perfusão/fisiologia
[Mh] Termos MeSH secundário: Oxigenação por Membrana Extracorpórea/métodos
Seres Humanos
Técnicas In Vitro
Espectrometria de Massas
Filtros Microporos
Modelos Biológicos
Gases Nobres/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Noble Gases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184212


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[PMID]:28237899
[Au] Autor:Zhai S; Zhao Y; Ji M; Qi W
[Ad] Endereço:School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China.
[Ti] Título:Simultaneous removal of nitrate and chromate in groundwater by a spiral fiber based biofilm reactor.
[So] Source:Bioresour Technol;232:278-284, 2017 May.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A spiral fiber based biofilm reactor was developed to remove nitrate and chromate simultaneously. The denitrification and Cr(VI) removal efficiency was evaluated with synthetic groundwater (NO -N=50mg/L) under different Cr(VI) concentrations (0-1.0mg/L), carbon nitrogen ratios (C/N) (0.8-1.2), hydraulic retention times (HRT) (2-16h) and initial pHs (4-10). Nitrate and Cr(VI) were completely removed without nitrite accumulation when the Cr(VI) concentration was lower than 0.4mg/L. As Cr(VI) up to 1.0mg/L, the system was obviously inhibited, but it recovered rapidly within 6days due to the strong adaption and domestication of microorganisms in the biofilm reactor. The results demonstrated that high removal efficiency of nitrate (≥99%) and Cr(VI) (≥95%) were achieved at lower C/N=0.9, HRT=8h, initial pH=7, and Cr(VI)=1.0mg/L. The technology proposed in present study can be alternative for simultaneous removal of co-contaminants in groundwater.
[Mh] Termos MeSH primário: Reatores Biológicos
Cromatos/isolamento & purificação
Água Subterrânea/química
Filtros Microporos
Nitratos/isolamento & purificação
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Carbono/química
Carbono/isolamento & purificação
Carbono/farmacocinética
Cromatos/farmacocinética
Desnitrificação
Nitratos/farmacocinética
Nitritos/isolamento & purificação
Nitritos/farmacocinética
Nitrogênio/química
Nitrogênio/isolamento & purificação
Nitrogênio/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromates); 0 (Nitrates); 0 (Nitrites); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


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[PMID]:28224696
[Au] Autor:Ordemann AG; Stanford JK; Sullivan DC; Reed JM
[Ad] Endereço:Department of Otolaryngology and Communicative Sciences, University of Mississippi Medical Center, Jackson, Mississippi, U.S.A.
[Ti] Título:Can contaminated water be rendered safe for nasal saline irrigations?
[So] Source:Laryngoscope;127(7):1513-1519, 2017 Jul.
[Is] ISSN:1531-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES/HYPOTHESIS: To compare sterile water to three methods of sterilization (carbon filtration, boiling, and ultraviolet [UV] light) for preparation of nasal saline irrigants free of bacterial and amebic contaminants. STUDY DESIGN: Bench-top translational research and cost comparison. METHODS: Sterile water was compared to common sterilization methods. Sterile water was contaminated with known concentrations of Staphylococcus aureus, Pseudomonas aeruginosa, Moraxella catarrhalis, Acinetobacter baumannii, Klebsiella pneumonia, Legionella pneumophila, and Naegleria fowleri. Test samples were subjected to boiling, carbon filtration, or ultraviolet light (UV) and then cultivated on appropriate media. Controls included samples of sterile water (negative control) and untreated test samples (positive control). RESULTS: Carbon filtration reduced but did not eliminate the number of organisms present in test samples. Boiling test samples for 5 minutes and UV light treatment resulted in sterilization of all organisms. Negative (sham contaminated) samples produced no growth, whereas positive (untreated) samples grew numerous organisms as expected. A cost comparison between bottled water and UV water sterilization (with SteriPEN Ultra) became equal in less than 2 years of consistent use. CONCLUSIONS: Carbon filtration reduces contamination but does not sterilize water and is thus unsafe for preparation of nasal saline irrigant. Boiling and UV treatment resulted in sterilization and are equivalent to purchased sterile water. Ultraviolet treatment was found to be safe, convenient, and a cost-effective alternative to purchased sterile water. LEVEL OF EVIDENCE: NA Laryngoscope, 127:1513-1519, 2017.
[Mh] Termos MeSH primário: Carvão Vegetal
Filtros Microporos
Lavagem Nasal/métodos
Cloreto de Sódio
Esterilização/métodos
Microbiologia da Água
Poluição da Água
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Carga Bacteriana
Seres Humanos
Pesquisa Médica Translacional
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
16291-96-6 (Charcoal); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1002/lary.26538


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[PMID]:27729703
[Au] Autor:Herbst DP
[Ad] Endereço:Department of Cardiac Surgery, Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Quebec, Canada.
[Ti] Título:Effects of Purge-Flow Rate on Microbubble Capture in Radial Arterial-Line Filters.
[So] Source:J Extra Corpor Technol;48(3):105-112, 2016 Sep.
[Is] ISSN:0022-1058
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The process of microbubble filtration from blood is complex and highly dependent on the forces of flow and buoyancy. To protect the patient from air emboli, arterial-line filters commonly use a micropore screen, a large volume housing with purpose-built shape, and a purge port to trap, separate, and remove circulating microbubbles. Although it has been proposed that an insufficient buoyancy force renders the purge port ineffective at removing microbubbles smaller than 500 µm, this research attempts to investigate the purge flow of an arterial-line filter to better understand the microbubble removal function in a typical radial filter design. As its primary objective, the study aims to determine the effect of purge-flow rate on bubble capture using air bolus injections from a syringe pump with 22-gauge needle and Doppler ultrasound bubble detection. The measureable bubble size generated in the test circuit ranged between 30 and 500 µm, while purge flow was varied between .1 and .5 L/min for testing. Statistical analysis of the test data was handled using a repeated measures design with significance set at < .05 level. Outcomes demonstrated that higher purge flows yielded higher bubble counts, but the effect of purge-flow rate on bubble capture decreased as bubble size increased. Results also showed that purge flow from the test filter was capable of capturing all bubble sizes being generated over the entire flow range tested, and confirms utility of the purge port in removing microbubbles smaller than 500 µm. By analyzing bubble counts in the purge flow of a typical radial-filter design, this study demonstrates that currently available micropore filter technology is capable of removing the size range of bubbles that commonly pass through modern pump-oxygenator systems and should continue to be considered during extracorporeal circulation as a measure to improve patient safety.
[Mh] Termos MeSH primário: Remoção de Componentes Sanguíneos/instrumentação
Velocidade do Fluxo Sanguíneo/fisiologia
Ponte Cardiopulmonar/instrumentação
Gases/isolamento & purificação
Filtros Microporos
Artéria Radial/fisiologia
[Mh] Termos MeSH secundário: Análise Química do Sangue/instrumentação
Desenho de Equipamento
Análise de Falha de Equipamento
Gases/química
Hemofiltração/instrumentação
Hemofiltração/métodos
Seres Humanos
Microbolhas
Ultrafiltração/instrumentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:T
[Da] Data de entrada para processamento:161013
[St] Status:MEDLINE


  5 / 2181 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:27163575
[Au] Autor:Basano Sde A; Medeiros JF; Fontes G; Vieira Gde D; Camargo JS; Vera LJ; Ferreira Rde G; Camargo LM
[Ad] Endereço:Centro de Medicina Tropical de Rondônia, Porto Velho, Rondônia, Brazil.
[Ti] Título:Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon.
[So] Source:Rev Soc Bras Med Trop;49(1):115-8, 2016 Feb.
[Is] ISSN:1678-9849
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longer-term residents (≥11 years; 60.2%). CONCLUSIONS M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.
[Mh] Termos MeSH primário: Mansonella/isolamento & purificação
Mansonelose/diagnóstico
Mansonelose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Brasil/epidemiologia
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Masculino
Mansonella/classificação
Filtros Microporos
Meia-Idade
Cimento de Policarboxilato
Prevalência
População Rural
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polycarboxylate Cement); 25766-59-0 (polycarbonate)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170105
[Lr] Data última revisão:
170105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


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[PMID]:27155911
[Au] Autor:Le Rouic JF; Breger D; Peronnet P; Hermouet-Leclair E; Alphandari A; Pousset-Decré C; Badat I; Becquet F
[Ad] Endereço:Clinique Sourdille, 3, place Anatole-France, 44000 Nantes, France. Electronic address: jflerouic@gmail.com.
[Ti] Título:[Extemporaneous withdrawal with a mini-spike filter: A low infection risk technique for drawing up bevacizumab for intravitreal injection].
[Ti] Título:Prélèvement extemporané du bévacizumab avec un mini-spike pour les injections intravitréennes : une technique de déconditionnement à risque infectieux minime..
[So] Source:J Fr Ophtalmol;39(5):415-20, 2016 May.
[Is] ISSN:1773-0597
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:PURPOSE: To describe a technique for extemporaneously drawing up bevacizumab for intravitreal injection (IVT) and report the rate of post-injection endophthtalmitis. PATIENTS AND METHODS: Retrospective monocentric analysis (January 2010-December 2014) of all IVT of bevacizumab drawn up with the following technique: in the operating room (class ISO 7) through a mini-spike with an integrated bacteria retentive air filter. The surgeon was wearing sterile gloves and a mask. The assisting nurse wore a mask. The bevacizumab vial was discarded at the end of each session. RESULTS: Six thousand two hundred and thirty-six bevacizumab injections were performed. One case of endophthalmitis was noted (0.016%). During the same period, 4 cases of endophthalmitis were found after IVT of other drugs (4/32,992; 0.012%. P=0.8). CONCLUSION: Intravitreal injection of bevacizumab after extemporaneous withdrawal through a mini-spike filter is a simple and safe technique. The risk of postoperative endophthalmitis is very low. This simple technique facilitates access to compounded bevacizumab.
[Mh] Termos MeSH primário: Inibidores da Angiogênese/administração & dosagem
Bevacizumab/administração & dosagem
Contaminação de Medicamentos/prevenção & controle
Infecções Oculares Bacterianas/prevenção & controle
Injeções Intravítreas/métodos
Degeneração Macular/tratamento farmacológico
Filtros Microporos
[Mh] Termos MeSH secundário: Inibidores da Angiogênese/efeitos adversos
Bevacizumab/efeitos adversos
Composição de Medicamentos/instrumentação
Composição de Medicamentos/métodos
Contaminação de Medicamentos/estatística & dados numéricos
Endoftalmite/epidemiologia
Endoftalmite/prevenção & controle
Infecções Oculares Bacterianas/epidemiologia
Infecções Oculares Bacterianas/etiologia
Seres Humanos
Injeções Intravítreas/efeitos adversos
Injeções Intravítreas/instrumentação
Injeções Intravítreas/normas
Complicações Pós-Operatórias/epidemiologia
Complicações Pós-Operatórias/prevenção & controle
Estudos Retrospectivos
Comportamento de Redução do Risco
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 2S9ZZM9Q9V (Bevacizumab)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160509
[St] Status:MEDLINE


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[PMID]:26905796
[Au] Autor:Woods GC; Sadmani AHMA; Andrews SA; Bagley DM; Andrews RC
[Ad] Endereço:Department of Civil Engineering, University of Toronto, 35 St. George Street, Toronto, ON M5S 1A4, Canada. Electronic address: Gwen.Woods-Chabane@HDRinc.com.
[Ti] Título:Rejection of pharmaceutically-based N-nitrosodimethylamine precursors using nanofiltration.
[So] Source:Water Res;93:179-186, 2016 Apr 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:N-Nitrosodimethylamine (NDMA) is a disinfection by-product (DBP) with many known precursors such as amine-containing pharmaceuticals that can enter the environment via treated wastewater. Reverse osmosis and tight nanofiltration membranes (MW cutoff < 200 Da) are treatment technologies that demonstrate high removal of many compounds, but at relatively high energy costs. Looser membranes (>200 Da) may provide sufficient removal of a wide range of contaminants with lower energy costs. This study examined the rejection of pharmaceuticals that are known NDMA precursors (∼300 Da) using nanofiltration (MW cutoff ∼350 Da). MQ water was compared to two raw water sources, and results illustrated that NDMA precursors (as estimated by formation potential testing) were effectively rejected in all water matrices (>84%). Mixtures of pharmaceuticals vs. single-spiked compounds were found to have no impact on rejection from the membranes used. The use of MQ water vs. surface waters illustrated that natural organic matter, colloids, and inorganic ions present did not significantly impact the rejection of the amine-containing pharmaceuticals. This study illustrates that NDMA formation potential testing can be effectively used for assessing NDMA precursor rejection from more complex samples with multiple and/or unknown NDMA precursors present, such as wastewater matrices.
[Mh] Termos MeSH primário: Dimetilnitrosamina/química
Pró-Fármacos/química
Águas Residuais/química
Poluentes Químicos da Água/química
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Cloraminas/química
Dimetilnitrosamina/isolamento & purificação
Desinfecção/instrumentação
Desinfecção/métodos
Filtração/instrumentação
Filtração/métodos
Membranas Artificiais
Filtros Microporos
Preparações Farmacêuticas/química
Preparações Farmacêuticas/isolamento & purificação
Pró-Fármacos/isolamento & purificação
Fatores de Tempo
Poluentes Químicos da Água/isolamento & purificação
Purificação da Água/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chloramines); 0 (Membranes, Artificial); 0 (Pharmaceutical Preparations); 0 (Prodrugs); 0 (Waste Water); 0 (Water Pollutants, Chemical); M43H21IO8R (Dimethylnitrosamine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


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[PMID]:26900973
[Au] Autor:Shan L; Fan H; Guo H; Ji S; Zhang G
[Ad] Endereço:Beijing Key Laboratory for Green Catalysis and Separation, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
[Ti] Título:Natural organic matter fouling behaviors on superwetting nanofiltration membranes.
[So] Source:Water Res;93:121-132, 2016 Apr 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nanofiltration has been widely recognized as a promising technology for the removal of micro-molecular organic components from natural water. Natural organic matter (NOM), a very important precursor of disinfection by-products, is currently considered as the major cause of membrane fouling. It is necessary to develop a membrane with both high NOM rejection and anti-NOM fouling properties. In this study, both superhydrophilic and superhydrophobic nanofiltration membranes for NOM removal have been fabricated. The fouling behavior of NOM on superwetting nanofiltration membranes has been extensively investigated by using humic acid (HA) as the model foulant. The extended Derjaguin-Landau-Verwey-Overbeek approach and nanoindentor scratch tests suggested that the superhydrophilic membrane had the strongest repulsion force to HA due to the highest positive total interaction energy (ΔG(TOT)) value and the lowest critical load. Excitation emission matrix analyses of natural water also indicated that the superhydrophilic membrane showed resistance to fouling by hydrophobic substances and therefore high removal thereof. Conversely, the superhydrophobic membrane showed resistance to fouling by hydrophilic substances and therefore high removal capacity. Long-term operation suggested that the superhydrophilic membrane had high stability due to its anti-NOM fouling capacity. Based on the different anti-fouling properties of the studied superwetting membranes, a combination of superhydrophilic and superhydrophobic membranes was examined to further improve the removal of both hydrophobic and hydrophilic pollutants. With a combination of superhydrophilic and superhydrophobic membranes, the NOM rejection (RUV254) and DOC removal rates (RDOC) could be increased to 83.6% and 73.3%, respectively.
[Mh] Termos MeSH primário: Incrustação Biológica
Membranas Artificiais
Compostos Orgânicos/química
Ultrafiltração/métodos
[Mh] Termos MeSH secundário: Algoritmos
Desinfetantes/química
Desinfetantes/isolamento & purificação
Desinfecção/instrumentação
Desinfecção/métodos
Substâncias Húmicas/análise
Interações Hidrofóbicas e Hidrofílicas
Filtros Microporos
Microscopia Eletrônica de Varredura
Modelos Químicos
Nanoestruturas/química
Nanoestruturas/ultraestrutura
Compostos Orgânicos/isolamento & purificação
Reprodutibilidade dos Testes
Espectrometria de Fluorescência
Ultrafiltração/instrumentação
Purificação da Água/instrumentação
Purificação da Água/métodos
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Disinfectants); 0 (Humic Substances); 0 (Membranes, Artificial); 0 (Organic Chemicals)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160223
[St] Status:MEDLINE


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[PMID]:26869432
[Au] Autor:Ölander M; Wisniewski JR; Matsson P; Lundquist P; Artursson P
[Ad] Endereço:Department of Pharmacy, Uppsala University, 751 23 Uppsala, Sweden.
[Ti] Título:The Proteome of Filter-Grown Caco-2 Cells With a Focus on Proteins Involved in Drug Disposition.
[So] Source:J Pharm Sci;105(2):817-827, 2016 Feb.
[Is] ISSN:1520-6017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Caco-2 cells are widely used in studies of intestinal cell physiology and drug transport. Here, the global proteome of filter-grown Caco-2 cells was quantified using the total protein approach and compared with the human colon and jejunum proteomes. In total, 8096 proteins were identified. In-depth analysis of proteins defining enterocyte differentiation-including brush-border hydrolases, integrins, and adherens and tight junctions-gave near-complete coverage of the expected proteins. Three hundred twenty-seven absorption, distribution, metabolism and excretion proteins were identified, including 112 solute carriers and 20 ATP-binding cassette transporters. OATP2B1 levels were 16-fold higher in Caco-2 cells than in jejunum. To investigate the impact of this difference on in vitro-in vivo extrapolations, we studied the uptake kinetics of the OATP2B1 substrate pitavastatin in Caco-2 monolayers, and found that the contribution of OATP2B1 was 60%-70% at clinically relevant intestinal concentrations. Pitavastatin kinetics was combined with transporter concentrations to model the contribution of active transport and membrane permeation in the jejunum. The lower OATP2B1 expression in jejunum led to a considerably lower transporter contribution (<5%), suggesting that transmembrane diffusion dominates pitavastatin absorption in vivo. In conclusion, we present the first in-depth quantification of the filter-grown Caco-2 proteome. We also demonstrate the crucial importance of considering transporter expression levels for correct interpretation of drug transport routes across the human intestine.
[Mh] Termos MeSH primário: Permeabilidade da Membrana Celular/fisiologia
Proteínas de Membrana/metabolismo
Filtros Microporos
Preparações Farmacêuticas/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Células CACO-2
Permeabilidade da Membrana Celular/efeitos dos fármacos
Colo/efeitos dos fármacos
Colo/metabolismo
Seres Humanos
Jejuno/efeitos dos fármacos
Jejuno/metabolismo
Preparações Farmacêuticas/administração & dosagem
Cimento de Policarboxilato/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (Pharmaceutical Preparations); 0 (Polycarboxylate Cement); 0 (Proteome); 25766-59-0 (polycarbonate)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE


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[PMID]:26838452
[Au] Autor:Mansour AA; Da Costa A; Arnaud T; Lu-Chau TA; Fdz-Polanco M; Moreira MT; Cacho Rivero JA
[Ad] Endereço:VEOLIA Research and Innovation, 291 Av. Dreyfous Ducas, F-78520, Limay, France. Electronic address: alicia.mansour@veolia.com.
[Ti] Título:Review of lignocellulolytic enzyme activity analyses and scale-down to microplate-based assays.
[So] Source:Talanta;150:629-37, 2016 Apr 01.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:With the increasing use of enzymes in environmental applications, there is a need for analytical methods adapted to large factorial experiments. Existing reference methods are chemical and labor intensive and unsuitable to analyze in parallel a large number of samples. Based on an extensive literature review and on experimental results, this work compares reference and microplate adapted methods to define the most adequate filter paper, carboxymethylcellulase, ß-glucosidase and xylanase activity tests. In the adapted methods, the total reaction volume was reduced from 2.2-24.5 mL to 0.21-0.24 mL. Statistical analysis of the activities measured on enzyme mixtures by applying the 96-well plate reduced methods showed that they were not significantly different to the activities obtained with reference tests.
[Mh] Termos MeSH primário: Bioensaio
Celulase/metabolismo
Técnicas de Química Analítica/instrumentação
Técnicas de Química Analítica/métodos
Endo-1,4-beta-Xilanases/metabolismo
Filtros Microporos
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.2.1.21 (beta-Glucosidase); EC 3.2.1.4 (Cellulase); EC 3.2.1.4 (carboxymethylcellulase); EC 3.2.1.8 (Endo-1,4-beta Xylanases)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE



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