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[PMID]:28508381
[Au] Autor:Wu K; Jia F; Zheng W; Luo Q; Zhao Y; Wang F
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences, Beijing, 100190, People's Republic of China.
[Ti] Título:Visualization of metallodrugs in single cells by secondary ion mass spectrometry imaging.
[So] Source:J Biol Inorg Chem;22(5):653-661, 2017 Jul.
[Is] ISSN:1432-1327
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Secondary ion mass spectrometry, including nanoscale secondary ion mass spectrometry (NanoSIMS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), has emerged as a powerful tool for biological imaging, especially for single cell imaging. SIMS imaging can provide information on subcellular distribution of endogenous and exogenous chemicals, including metallodrugs, from membrane through to cytoplasm and nucleus without labeling, and with high spatial resolution and chemical specificity. In this mini-review, we summarize recent progress in the field of SIMS imaging, particularly in the characterization of the subcellular distribution of metallodrugs. We anticipate that the SIMS imaging method will be widely applied to visualize subcellular distributions of drugs and drug candidates in single cells, exerting significant influence on early drug evaluation and metabolism in medicinal and pharmaceutical chemistry. Recent progress of SIMS applications in characterizing the subcellular distributions of metallodrugs was summarized.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Compostos Organometálicos/farmacologia
Análise de Célula Única
Espectrometria de Massa de Íon Secundário
[Mh] Termos MeSH secundário: Antineoplásicos/química
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Compostos Organometálicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Organometallic Compounds)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1007/s00775-017-1462-3


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[PMID]:28253633
[Au] Autor:Cizinauskas V; Elie N; Brunelle A; Briedis V
[Ad] Endereço:Department of Clinical Pharmacy, Lithuanian University of Health Sciences, Sukileliu pr. 13, 50166 Kaunas, Lithuania.
[Ti] Título:Fatty acids penetration into human skin ex vivo: A TOF-SIMS analysis approach.
[So] Source:Biointerphases;12(1):011003, 2017 Mar 02.
[Is] ISSN:1559-4106
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Linoleic, oleic, palmitoleic, palmitic, and stearic fatty acids (FAs) are commonly used in dermatological formulations. They differ by their structure, presence in the skin, and mode of application in pharmaceuticals and cosmetics compounding. These FAs are also known as chemical penetration enhancers, but their mechanisms of penetration enhancement and effect on barrier characteristics of the skin require additional study. In this study, the authors conducted an ex vivo analysis of the distribution of lipid components in the epidermis and dermis of human skin after applying individual FAs. The goal was to elucidate possible mechanisms of penetration enhancement and FA effects on barrier characteristics of the skin. FA penetration studies were conducted ex vivo on human skin and time-of-flight secondary ion mass spectrometry (TOF-SIMS) bioimaging analysis was performed to visualize and analyze distribution of FAs in skin sections. The current study demonstrated that TOF-SIMS imaging was effective in visualizing the distribution of linoleic, oleic, palmitoleic, palmitic, and stearic acid in the human skin ex vivo after the skin penetration experiment of individual FAs. The integration of the obtained TOF-SIMS images allowed a semiquantitative comparison of the effects induced by individual FA applications on the human skin ex vivo. FAs showed varying abilities to penetrate the skin and disorder the FAs within the skin, based on their structures and physicochemical properties. Linoleic acid penetrated the skin and changed the distribution of all the analyzed FAs. Skin treatment with palmitoleic or oleic acid increased the amounts of singular FAs in the skin. Penetration of saturated FAs was low, but it increased the detected amounts of linoleic acid in both skin layers. The results indicate that application of FAs on the skin surface induce redistribution of native FAs not only in the stratum corneum layer of epidermis but also in the lipid content of full epidermis and dermis layers. The results indicate that topically applied pharmaceutical products should be evaluated for potential chemical penetration enhancement and lipid component redistribution effects during formulation.
[Mh] Termos MeSH primário: Derme/efeitos dos fármacos
Epiderme/efeitos dos fármacos
Ácidos Graxos/farmacocinética
[Mh] Termos MeSH secundário: Derme/química
Epiderme/química
Ácidos Graxos/análise
Seres Humanos
Imagem Óptica
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1116/1.4977941


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[PMID]:28183674
[Au] Autor:Höhn S; Braem A; Neirinck B; Virtanen S
[Ad] Endereço:Institute for Surface Science and Corrosion, Dept. of Mat. Science, University of Erlangen-Nürnberg, 91058 Erlangen, Germany.. Electronic address: sarah.hoehn@fau.de.
[Ti] Título:Albumin coatings by alternating current electrophoretic deposition for improving corrosion resistance and bioactivity of titanium implants.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:798-807, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Although Ti alloys are generally regarded to be highly corrosion resistant, inflammatory conditions following surgery can instigate breakdown of the TiO passivation layer leading to an increased metal ion release. Furthermore proteins present in the surrounding tissue will readily adsorb on a titanium surface after implantation. In this paper alternating current electrophoretic deposition (AC-EPD) of bovine serum albumin (BSA) on Ti6Al4V was investigated in order to increase the corrosion resistance and control the protein adsorption capability of the implant surface. The Ti6Al4V surface was characterized with SEM, XPS and ToF-SIMS after long-term immersion tests under physiological conditions and simulated inflammatory conditions either in Dulbecco's Modified Eagle Medium (DMEM) or DMEM supplemented with fetal calf serum (FCS). The analysis showed an increased adsorption of amino acids and proteins from the different immersion solutions. The BSA coating was shown to prevent selective dissolution of the vanadium (V) rich ß-phase, thus effectively limiting metal ion release to the environment. Electrochemical impedance spectroscopy measurements confirmed an increase of the corrosion resistance for BSA coated surfaces as a function of immersion time due to the time-dependent adsorption of the different amino acids (from DMEM) and proteins (from FCS) as observed by ToF-SIMS analysis.
[Mh] Termos MeSH primário: Materiais Revestidos Biocompatíveis/farmacologia
Eletricidade
Eletroforese
Próteses e Implantes
Soroalbumina Bovina/farmacologia
Titânio/farmacologia
[Mh] Termos MeSH secundário: Animais
Cálcio/análise
Bovinos
Corrosão
Espectroscopia Dielétrica
Nanopartículas/ultraestrutura
Fósforo/análise
Espectroscopia Fotoeletrônica
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 12743-70-3 (titanium alloy (TiAl6V4)); 27432CM55Q (Serum Albumin, Bovine); 27YLU75U4W (Phosphorus); D1JT611TNE (Titanium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


  4 / 1191 MEDLINE  
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[PMID]:28176531
[Au] Autor:Wright P; Eckers C
[Ad] Endereço:Smithers Rapra, Shawbury, Shropshire, SY4 4NR, UK.
[Ti] Título:37th British Mass Spectrometry Society annual meeting.
[So] Source:Bioanalysis;9(5):423-426, 2017 Mar.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 37th British Mass Spectrometry Society (BMSS) annual meeting took place over a brilliantly sunny 3 days by the sea in the historic Eastbourne Winter Gardens on the south coast of England. It was held between 13 and 15 September 2016. Two-hundred attendees enjoyed a conference covering all aspects of MS with speakers drawn from across Europe and North America. The BMSS is particularly proud of the encouragement it offers students and early career scientists, both financially in the form of travel grants and also in terms of opportunities to present at an international level in a supportive atmosphere. Further encouragement to newcomers to the field is offered in the form of the Barber Prize for best oral presentation and the Bordoli Prize for the best poster. This year's winners were Patrick Knight (University of Leeds), the Bordoli Prize for the poster 'Characterising the Interaction of Ataxin-3 and the Poly-Glutamine Aggregation Inhibitor QBP1'; Lisa Deininger (Sheffield Hallam University), the Barber Prize for 'Out Damned Spot! Bottom Up Proteomics for the Analysis of Bloodied Fingermarks'. In addition, the Delegates Choice for best poster went to Hannah Britt (Durham University) for 'Monitoring Reactions of Small Molecules with Cell Membranes by Liquid Chromatography-Mass Spectrometry'. For services to the MS community, Professor Gareth Brenton (Swansea University) was awarded the BMSS Medal; Professor Alison Ashcroft (Leeds University) and Anna Upton (former BMSS administrator) were given lifetime membership of the BMSS.
[Mh] Termos MeSH primário: Espectrometria de Massas
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Inglaterra
Preparações Farmacêuticas/análise
Sociedades Científicas
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:CONGRESSES
[Nm] Nome de substância:
0 (Pharmaceutical Preparations)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2016-4981


  5 / 1191 MEDLINE  
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[PMID]:28154861
[Au] Autor:Lee RFS; Theiner S; Meibom A; Koellensperger G; Keppler BK; Dyson PJ
[Ad] Endereço:Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), CH1-1015 Lausanne, Switzerland. paul.dyson@epfl.ch.
[Ti] Título:Application of imaging mass spectrometry approaches to facilitate metal-based anticancer drug research.
[So] Source:Metallomics;9(4):365-381, 2017 Apr 19.
[Is] ISSN:1756-591X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mass spectrometry imaging is being increasingly used in metal-based anticancer drug development to study elemental and/or molecular drug distributions in different biological systems. The main analytical tools employed are SIMS (especially nanoSIMS), LA-ICP-MSI and MALDI-MSI as well as a combination of complementary imaging techniques. Main challenges are appropriate sample preparation methods, reliable and validated quantification strategies and a trade-off between sensitivity and spatial resolution. So far, research has mostly focused on the development of analytical methods for imaging with the long term goal to study drug uptake into tumor tissue and toxicity affected organs and to identify cellular targets of metal-based drugs. In this review we cover the technological features of the mass spectrometry imaging methods used and give an overview of the applications in metal-based anticancer drug research as well as some future perspectives.
[Mh] Termos MeSH primário: Antineoplásicos/análise
Descoberta de Drogas/métodos
Espectrometria de Massas/métodos
Metais/análise
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacocinética
Seres Humanos
Metais/farmacocinética
Neoplasias/tratamento farmacológico
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Espectrometria de Massa de Íon Secundário/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Metals)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1039/c6mt00231e


  6 / 1191 MEDLINE  
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[PMID]:28146236
[Au] Autor:Pilgrim MG; Lengyel I; Lanzirotti A; Newville M; Fearn S; Emri E; Knowles JC; Messinger JD; Read RW; Guidry C; Curcio CA
[Ad] Endereço:UCL Institute of Ophthalmology, University College London, London, United Kingdom 2Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, United Kingdom.
[Ti] Título:Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model.
[So] Source:Invest Ophthalmol Vis Sci;58(2):708-719, 2017 Feb 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Extracellular deposits containing hydroxyapatite, lipids, proteins, and trace metals that form between the basal lamina of the RPE and the inner collagenous layer of Bruch's membrane are hallmarks of early AMD. We examined whether cultured RPE cells could produce extracellular deposits containing all of these molecular components. Methods: Retinal pigment epithelium cells isolated from freshly enucleated porcine eyes were cultured on Transwell membranes for up to 6 months. Deposit composition and structure were characterized using light, fluorescence, and electron microscopy; synchrotron x-ray diffraction and x-ray fluorescence; secondary ion mass spectroscopy; and immunohistochemistry. Results: Apparently functional primary RPE cells, when cultured on 10-µm-thick inserts with 0.4-µm-diameter pores, can produce sub-RPE deposits that contain hydroxyapatite, lipids, proteins, and trace elements, without outer segment supplementation, by 12 weeks. Conclusions: The data suggest that sub-RPE deposit formation is initiated, and probably regulated, by the RPE, as well as the loss of permeability of the Bruch's membrane and choriocapillaris complex associated with age and early AMD. This cell culture model of early AMD lesions provides a novel system for testing new therapeutic interventions against sub-RPE deposit formation, an event occurring well in advance of the onset of vision loss.
[Mh] Termos MeSH primário: Durapatita/metabolismo
Células Epiteliais/metabolismo
Epitélio Pigmentado Ocular/metabolismo
Drusas Retinianas/metabolismo
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Fluorescência
Imuno-Histoquímica
Degeneração Macular/metabolismo
Microscopia Eletrônica
Epitélio Pigmentado Ocular/citologia
Cultura Primária de Células
Espectrometria de Massa de Íon Secundário
Suínos
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21060


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[PMID]:27913138
[Au] Autor:Grivès S; Phan G; Bouvier-Capely C; Suhard D; Rebière F; Agarande M; Fattal E
[Ad] Endereço:Université Paris-Sud, Faculté de pharmacie, Institut Galien Paris-Sud, LabEx LERMIT, 5 rue JB Clément, 92296 Châtenay-Malabry Cedex, France; CNRS, UMR 8612, 5 rue JB Clément, 92296 Châtenay-Malabry Cedex, France; Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-Hom, SDI, Laboratoire de
[Ti] Título:Compared in vivo efficiency of nanoemulsions unloaded and loaded with calixarene and soapy water in the treatment of superficial wounds contaminated by uranium.
[So] Source:Chem Biol Interact;267:33-39, 2017 Apr 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:No emergency decontamination treatment is currently available in the case of radiological skin contamination by uranium compounds. First responders in the workplace or during an industrial nuclear accident must be able to treat internal contamination through skin. For this purpose, a calixarene nanoemulsion was developed for the treatment of intact skin or superficial wounds contaminated by uranium, and the decontamination efficiency of this nanoemulsion was investigated in vitro and ex vivo. The present work addresses the in vivo decontamination efficiency of this nanoemulsion, using a rat model. This efficiency is compared to the radio-decontaminant soapy water currently used in France (Trait rouge ) in the workplace. The results showed that both calixarene-loaded nanoemulsion and non-loaded nanoemulsion allowed a significant decontamination efficiency compared to the treatment with soapy water. Early application of the nanoemulsions on contaminated excoriated rat skin allowed decreasing the uranium content by around 85% in femurs, 95% in kidneys and 93% in urines. For skin wounded by microneedles, mimicking wounds by microstings, nanoemulsions allowed approximately a 94% decrease in the uranium retention in kidneys. However, specific chelation of uranium by calixarene molecules within the nanoemulsion was not statistically significant, probably because of the limited calixarene-to-uranium molar ratio in these experiment conditions. Moreover, these studies showed that the soapy water treatment potentiates the transcutaneous passage of uranium, thus making it bioavailable, in particular when the skin is superficially wounded.
[Mh] Termos MeSH primário: Calixarenos/farmacologia
Nanoestruturas/química
Substâncias Protetoras/farmacologia
Pele/efeitos dos fármacos
Sabões/farmacologia
Urânio/toxicidade
[Mh] Termos MeSH secundário: Animais
Calixarenos/química
Descontaminação
Rim/efeitos dos fármacos
Rim/patologia
Masculino
Ratos
Ratos Sprague-Dawley
Pele/patologia
Sabões/química
Espectrometria de Massa de Íon Secundário
Água/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protective Agents); 0 (Soaps); 059QF0KO0R (Water); 130036-26-9 (Calixarenes); 4OC371KSTK (Uranium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE


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[PMID]:27889387
[Au] Autor:Holmes AJ; Chew YV; Colakoglu F; Cliff JB; Klaassens E; Read MN; Solon-Biet SM; McMahon AC; Cogger VC; Ruohonen K; Raubenheimer D; Le Couteur DG; Simpson SJ
[Ad] Endereço:Charles Perkins Centre, University of Sydney, NSW 2006, Australia; School of Life and Environmental Science, University of Sydney, NSW 2006, Australia. Electronic address: andrew.holmes@sydney.edu.au.
[Ti] Título:Diet-Microbiome Interactions in Health Are Controlled by Intestinal Nitrogen Source Constraints.
[So] Source:Cell Metab;25(1):140-151, 2017 Jan 10.
[Is] ISSN:1932-7420
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diet influences health and patterns of disease in populations. How different diets do this and why outcomes of diets vary between individuals are complex and involve interaction with the gut microbiome. A major challenge for predicting health outcomes of the host-microbiome dynamic is reconciling the effects of different aspects of diet (food composition or intake rate) on the system. Here we show that microbial community assembly is fundamentally shaped by a dichotomy in bacterial strategies to access nitrogen in the gut environment. Consequently, the pattern of dietary protein intake constrains the host-microbiome dynamic in ways that are common to a very broad range of diet manipulation strategies. These insights offer a mechanism for the impact of high protein intake on metabolic health and form the basis for a general theory of the impact of different diet strategies on host-microbiome outcomes.
[Mh] Termos MeSH primário: Dieta
Saúde
Intestinos/metabolismo
Microbiota
Nitrogênio/metabolismo
[Mh] Termos MeSH secundário: Animais
Bactérias/efeitos dos fármacos
Bactérias/metabolismo
Biodiversidade
Simulação por Computador
Carboidratos da Dieta/farmacologia
Proteínas na Dieta/farmacologia
Metabolismo Energético/efeitos dos fármacos
Alimentos
Intestinos/efeitos dos fármacos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Microbiota/efeitos dos fármacos
Mucinas/metabolismo
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Carbohydrates); 0 (Dietary Proteins); 0 (Mucins); N762921K75 (Nitrogen)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE


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[PMID]:27847223
[Au] Autor:Gajos K; Budkowski A; Pagkali V; Petrou P; Biernat M; Awsiuk K; Rysz J; Bernasik A; Misiakos K; Raptis I; Kakabakos S
[Ad] Endereço:M. Smoluchowski Institute of Physics, Jagiellonian University, Lojasiewicza 11, 30-348 Kraków, Poland. Electronic address: katarzyna.gajos@doctoral.uj.edu.pl.
[Ti] Título:Indirect immunoassay on functionalized silicon surface: Molecular arrangement, composition and orientation examined step-by-step with multi-technique and multivariate analysis.
[So] Source:Colloids Surf B Biointerfaces;150:437-444, 2017 Feb 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The arrangement, composition and orientation of immunoreagents employed in an indirect immunoassay for determination of mycotoxin OchraToxin A (OTA) are specified for Si N substrate, aiming to imitate biosensor transducers made of the same material. Si N surfaces are examined after modification with (3-aminopropyl)triethoxysilane, spotting with OTA-ovalbumin conjugate (probe), blocking with bovine serum albumin, reaction with a mouse monoclonal antibody against OTA and, finally, reaction with a goat anti-mouse secondary antibody. Atomic force micrographs, their autocorrelation and height histogram parameters, show the stepwise development of a multi-component monolayer covered by groups of secondary antibody molecules. Time-Of-Flight Secondary Ion Mass Spectrometry reveals the composition of probe and blocking protein, as well as their partial desorption during the primary immunoreaction. Ellipsometry provides surface amount of all proteins, increasing step-by-step from 0.7 to 6.9mg/m . In addition, ellipsometry combined with TOF-SIMS reveals the mass loadings of different molecules in the intermediate and the final overlayer. Based on this, some orientations of the immobilized molecules are proposed and a molar ratio of ∼2.5 for secondary to primary antibody is calculated. The orientations of the primary and secondary antibody are further clarified by Principal Component Analysis of TOF-SIMS data, through which a side-on and a head-on orientation is deduced for the primary and the secondary antibody, respectively. These findings demonstrate how the combination of multiple surface analysis techniques can provide insight on the arrangement, composition and orientation of biomolecules in the course of multi-step procedures employed in biosensors.
[Mh] Termos MeSH primário: Ocratoxinas/química
Silício/química
[Mh] Termos MeSH secundário: Animais
Técnicas Biossensoriais
Bovinos
Imunoensaio/métodos
Imunoglobulina G/química
Camundongos
Microscopia de Força Atômica
Análise Multivariada
Análise de Componente Principal
Propilaminas/química
Soroalbumina Bovina/química
Silanos/química
Espectrometria de Massa de Íon Secundário
Eletricidade Estática
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Ochratoxins); 0 (Propylamines); 0 (Silanes); 1779SX6LUY (ochratoxin A); 27432CM55Q (Serum Albumin, Bovine); L8S6UBW552 (amino-propyl-triethoxysilane); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE


  10 / 1191 MEDLINE  
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[PMID]:27107265
[Au] Autor:França R; Alfa M; Olson N; Yahia L; Sacher E
[Ad] Endereço:Dental Biomaterials Research Laboratory, Faculty of Health Sciences, Department of Restorative Dentistry, University of Manitoba, 780 Bannatyne Avenue, Winnipeg, Manitoba R3E 0W2, Canada.
[Ti] Título:Characterization of endotoxins on orthopaedic fixation screws, using physicochemical surface analyses.
[So] Source:J Orthop Res;35(2):240-247, 2017 Feb.
[Is] ISSN:1554-527X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to determine if surface analysis techniques could be used to detect endotoxin on stainless steel malleolus screws. New malleolus screws were compared to ones that had been coated in purified lipopolysaccharide (LPS) or Artificial Test Soil (ATS) containing lipopolysaccharide. X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and time-of flight secondary ion mass spectrometry (TOF-SIMS) were used to assess the fixation screws surface. Organic material was visualized on the LPS and ATS-LPS inoculated screws but not on the new unsoiled screws. This was further supported by the peaks observed at masses between 40 and 100 D in TOF-SIMS spectra of the LPS and ATS-LPS inoculated screws. After deconvolution of N1s high resolution XPS spectra, the LPS inoculated screws showed amide groups whereas the ATS-LPS inoculated screws showed predominantly nitroso groups (C-NO). Our data demonstrate that surface analysis can be used to detect organic residuals present on fixation screws. The XPS data confirmed that LPS reacted predominantly with positively charged surface metallic ions (Fe and Cr), whereas proteins reacted with the surface oxide layer of fixation screws, forming C-NO groups. The application of these surface analysis techniques will be helpful in determining if the reprocessing of such items results in an accumulation of organic material that might lead to aseptic loosening, when implanted. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:240-247, 2017.
[Mh] Termos MeSH primário: Parafusos Ósseos
Endotoxinas/análise
Fixação Interna de Fraturas/instrumentação
[Mh] Termos MeSH secundário: Contaminação de Equipamentos
Lipopolissacarídeos
Microscopia Eletrônica de Varredura
Espectroscopia Fotoeletrônica
Espectrometria de Massa de Íon Secundário
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endotoxins); 0 (Lipopolysaccharides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160424
[St] Status:MEDLINE
[do] DOI:10.1002/jor.23271



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