Base de dados : MEDLINE
Pesquisa : E05.197.124 [Categoria DeCS]
Referências encontradas : 2274 [refinar]
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[PMID]:29289887
[Au] Autor:Moussa G; Alaaeddine R; Alaeddine LM; Nassra R; Belal ASF; Ismail A; El-Yazbi AF; Abdel-Ghany YS; Hazzaa A
[Ad] Endereço:Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
[Ti] Título:Novel click modifiable thioquinazolinones as anti-inflammatory agents: Design, synthesis, biological evaluation and docking study.
[So] Source:Eur J Med Chem;144:635-650, 2018 Jan 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Click chemistry was used to synthesize a new series of thioquinazolinone molecules equipped with propargyl moiety,1,2,3-triazolyl and isoxazolyl rings. Our design was based on merging pharmacophores previously reported to exhibit COX-2 inhibitory activities to a thioquinazolinone-privileged scaffold. The synthesized compounds were subjected to in vitro cyclooxygenase COX-1/COX-2 and 15-LOX inhibition assays. Compounds 2c, 3b, 3h, 3j, and 3k showed COX-2 inhibition with IC (µM) 0.18, 0.19, 0.11, 0.16 and 0.17 respectively. These values were compared to celecoxib (IC 0.05 µM), diclofenac (IC 0.8 µM) and indomethacin (IC 0.49 µM) reference drugs. They also showed 15-LOX inhibition with IC (µM) 6.21, 4.33, 7.62, 5.21 and 3.98 respectively. These values were compared with Zileuton (IC 2.41 µM) and Meclofenamate sodium (IC 5.64 µM) as positive controls. These compounds were further challenged by PMA-induced THP-1 differentiation assay where compounds 2c and 3j inhibited monocyte to macrophage differentiation efficiently with IC values of 4.78 µM and 5.63 µM, respectively, compared to that of diclofenac sodium (4.86 µM). On the other hand, 3h demonstrated a significantly increased potency compared to diclofenac in this assay (IC = 0.13 µM). The same compounds exhibited significant in vivo anti-inflammatory effect as indicated by the formalin-induced rat-paw edema test. Docking experiments of compounds 2c, 3b, 3h, 3j, and 3k into COX-2 binding pocket have been conducted, where strong binding interactions have been identified and effective overall docking scores have been recorded. Their drug-likeness has been assessed using Molinspiration, Molsoft and Pre-ADMET software products.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia
Inibidores de Ciclo-Oxigenase/farmacologia
Desenho de Drogas
Inibidores de Lipoxigenase/farmacologia
Quinazolinonas/farmacologia
Compostos de Sulfidrila/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/síntese química
Anti-Inflamatórios não Esteroides/química
Araquidonato 15-Lipoxigenase/metabolismo
Diferenciação Celular/efeitos dos fármacos
Química Click
Ciclo-Oxigenase 1/metabolismo
Ciclo-Oxigenase 2/metabolismo
Inibidores de Ciclo-Oxigenase/síntese química
Inibidores de Ciclo-Oxigenase/química
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Inibidores de Lipoxigenase/síntese química
Inibidores de Lipoxigenase/química
Macrófagos/efeitos dos fármacos
Simulação de Acoplamento Molecular
Estrutura Molecular
Quinazolinonas/síntese química
Quinazolinonas/química
Ratos
Ratos Wistar
Relação Estrutura-Atividade
Compostos de Sulfidrila/síntese química
Compostos de Sulfidrila/química
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cyclooxygenase Inhibitors); 0 (Lipoxygenase Inhibitors); 0 (Quinazolinones); 0 (Sulfhydryl Compounds); EC 1.13.11.33 (Arachidonate 15-Lipoxygenase); EC 1.14.99.1 (Cyclooxygenase 1); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS1 protein, human); EC 1.14.99.1 (PTGS2 protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180101
[St] Status:MEDLINE


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[PMID]:29324340
[Au] Autor:Brandão GC; Rocha Missias FC; Arantes LM; Soares LF; Roy KK; Doerksen RJ; Braga de Oliveira A; Pereira GR
[Ad] Endereço:Departamento de Farmácia, Escola de Farmácia, UFOP, Campus Morro do Cruzeiro, s/n, Balxita, CEP 35400-000, Ouro Preto, MG, Brazil.
[Ti] Título:Antimalarial naphthoquinones. Synthesis via click chemistry, in vitro activity, docking to PfDHODH and SAR of lapachol-based compounds.
[So] Source:Eur J Med Chem;145:191-205, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Lapachol is an abundant prenyl naphthoquinone occurring in Brazilian Bignoniaceae that was clinically used, in former times, as an antimalarial drug, despite its moderate effect. Aiming to search for potentially better antimalarials, a series of 1,2,3-triazole derivatives was synthesized by chemical modification of lapachol. Alkylation of the hydroxyl group gave its propargyl ether which, via copper-catalyzed cycloaddition (CuAAC) click chemistry with different organic azides, afforded 17 naphthoquinonolyl triazole derivatives. All the synthetic compounds were evaluated for their in vitro activity against chloroquine resistant Plasmodium falciparum (W2) and for cytotoxicity to HepG2 cells. Compounds containing the naphthoquinolyl triazole moieties showed higher antimalarial activity than lapachol (IC 123.5 µM) and selectivity index (SI) values in the range of 4.5-197.7. Molecular docking simulations of lapachol, atovaquone and all the newly synthesized compounds were carried out for interactions with PfDHODH, a mitochondrial enzyme of the parasite respiratory chain that is essential for de novo pyrimidine biosynthesis. Docking of the naphthoquinonolyl triazole derivatives to PfDHODH yielded scores between -9.375 and -14.55 units, compared to -9.137 for lapachol and -12.95 for atovaquone and disclosed the derivative 17 as a lead compound. Therefore, the study results show the enhancement of DHODH binding affinity correlated with improvement of SI values and in vitro activities of the lapachol derivatives.
[Mh] Termos MeSH primário: Antimaláricos/farmacologia
Naftoquinonas/farmacologia
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antimaláricos/síntese química
Antimaláricos/química
Sobrevivência Celular/efeitos dos fármacos
Cloroquina/farmacologia
Química Click
Relação Dose-Resposta a Droga
Resistência a Medicamentos/efeitos dos fármacos
Células Hep G2
Seres Humanos
Modelos Moleculares
Estrutura Molecular
Naftoquinonas/química
Testes de Sensibilidade Parasitária
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimalarials); 0 (Naphthoquinones); 886U3H6UFF (Chloroquine); B221938VB6 (lapachol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


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[PMID]:28457141
[Au] Autor:Liu X; Zhang P; Rödl W; Maier K; Lächelt U; Wagner E
[Ad] Endereço:Pharmaceutical Biotechnology, Center for System-based Drug Research and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München , Butenandtstrasse 5-13, D-81377 Munich, Germany.
[Ti] Título:Toward Artificial Immunotoxins: Traceless Reversible Conjugation of RNase A with Receptor Targeting and Endosomal Escape Domains.
[So] Source:Mol Pharm;14(5):1439-1449, 2017 May 01.
[Is] ISSN:1543-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The specific transport of bioactive proteins into designated target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A via this polycation-free delivery strategy resulted in high cytotoxicity against receptor-positive KB tumor cells only when both PEG-folate and INF7 were attached.
[Mh] Termos MeSH primário: Química Click/métodos
Endossomos/metabolismo
Imunotoxinas/química
Ribonuclease Pancreático/química
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Eletroforese em Gel de Poliacrilamida
Ácido Fólico/análogos & derivados
Ácido Fólico/química
Seres Humanos
Concentração de Íons de Hidrogênio
Imunotoxinas/metabolismo
Modelos Biológicos
Peptídeos/química
Polietilenoglicóis/química
Ribonuclease Pancreático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (INF7 peptide); 0 (Immunotoxins); 0 (Peptides); 0 (poly(ethylene glycol)-folate); 30IQX730WE (Polyethylene Glycols); 935E97BOY8 (Folic Acid); EC 3.1.27.5 (Ribonuclease, Pancreatic)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1021/acs.molpharmaceut.6b00701


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[PMID]:29025662
[Au] Autor:Li S; Wang L; Yu X; Wang C; Wang Z
[Ad] Endereço:Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, PR China.
[Ti] Título:Synthesis and characterization of a novel double cross-linked hydrogel based on Diels-Alder click reaction and coordination bonding.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:299-309, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hydrogels, promising biological materials, need to have both strong mechanical properties and also inherent self-healing properties. In this work a double cross-linked network (DN) hydrogel was designed and prepared by combining a Diels-Alder click reaction and coordination effects. This DN hydrogel had good thermodynamic properties, anti-EDTA performance and self-healing properties. In addition, the mechanical properties, swelling properties and surface morphology of DN hydrogels can be controlled by adjusting the ratio of Fe -catechol. The adjustment of pH value can change the color, crosslinking mode and mechanical properties of the DN hydrogel. This smart hydrogel created from DA click chemistry and coordination effects has significance for guiding the design of new hydrogels with good mechanical properties, self-healing properties and controlled cross-link density.
[Mh] Termos MeSH primário: Hidrogéis/química
[Mh] Termos MeSH secundário: Varredura Diferencial de Calorimetria
Catecóis/química
Quitosana/química
Química Click
Reação de Cicloadição
Compostos Férricos/química
Hidrogéis/síntese química
Concentração de Íons de Hidrogênio
Espectroscopia de Ressonância Magnética
Reologia
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Termogravimetria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 0 (Ferric Compounds); 0 (Hydrogels); 9012-76-4 (Chitosan); LF3AJ089DQ (catechol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  5 / 2274 MEDLINE  
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[PMID]:27775109
[Au] Autor:Zhang XR; Zhang Y; Chen FT; Li Y; Zhang SS
[Ad] Endereço:Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
[Ti] Título:Visual detection of single-nucleotide polymorphisms and DNA methyltransferase based on cation-exchange of CuS nanoparticles and click chemistry of functionalized gold nanoparticles.
[So] Source:Chem Commun (Camb);52(90):13261-13264, 2016 Nov 03.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs). As a proof-of-principle, novel applications of this method as a versatile biosensor for single-nucleotide polymorphisms (SNPs) and DNA methyltransferase (MTase) were presented.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Calorimetria/métodos
Cobre/química
Metilases de Modificação do DNA/metabolismo
Ouro/química
Nanopartículas Metálicas/química
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Química Click
Troca Iônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7440-57-5 (Gold); 789U1901C5 (Copper); EC 2.1.1.- (DNA Modification Methylases); KL4YU612X7 (cupric sulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE


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[PMID]:27778291
[Au] Autor:Soriano GP; Overkleeft HS; Florea BI
[Ad] Endereço:Bio-organic Synthesis Group, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.
[Ti] Título:Two-Step Activity-Based Protein Profiling with the Proteasome System as Model of Study.
[So] Source:Methods Mol Biol;1491:205-215, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Activity-based protein profiling (ABPP) is a method to highlight enzymatic activities in a biological sample, which uses chemical probes that react covalently with the catalytic nucleophile of the enzyme. To circumvent disadvantages associated with the presence of reporter tags on chemical probes, the probe is equipped with a ligation handle to which the reporter can be reacted at the desired time and place in the ABPP workflow. This chapter demonstrates the power of a triple bioorthogonal ligation strategy which addresses the three activities of the proteasome: the ß5-subunit selective norbornene-tagged probe is reacted with fluorescent tetrazine, the ß1-selective azide-functionalized probe was addressed with a biotinylated phosphine, followed by an alkyne-substituted pan-reactive probe to label the remaining ß2 activity to which an azide-coupled fluorophore was ligated. The result of the triple ligation was similar to each reaction performed separately demonstrating the value of the triple ligation strategy for a single experiment.
[Mh] Termos MeSH primário: Modelos Químicos
Complexo de Endopeptidases do Proteassoma/química
Proteínas/química
[Mh] Termos MeSH secundário: Azidas/química
Química Click
Células HEK293
Seres Humanos
Sondas Moleculares/química
Complexo de Endopeptidases do Proteassoma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (Molecular Probes); 0 (Proteins); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:27778282
[Au] Autor:Ravindran MS; Wenk MR
[Ad] Endereço:NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore (NUS), Singapore, 117456, Singapore.
[Ti] Título:Activity-Based Lipid Esterase Profiling of M. bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait.
[So] Source:Methods Mol Biol;1491:75-85, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This chapter provides a step-by-step protocol using activity-based protein profiling (ABPP) as a chemical-proteomic tool to survey the antibiotic properties of a small molecule. Here, we investigate the molecular mechanism behind the bactericidal activity of tetrahydrolipstatin (THL). ABPP relies on small molecule probes that target the active site of specific enzymes in complex proteomes. These probes in turn are equipped with a reporter tag that allows capturing, visualization, enrichment, identification, and quantification of its targets either in vitro or in situ. THL possesses bactericidal activities, but its precise spectrum of molecular targets is poorly characterized. Here, we used THL analogs functionalized to enable Huisgen-base cycloaddition, commonly known as "click chemistry," to identify target proteins after enrichment from mycobacterial cell lysates obtained from different physiological conditions.
[Mh] Termos MeSH primário: Esterases/metabolismo
Lactonas/farmacologia
Metabolismo dos Lipídeos
Mycobacterium bovis/enzimologia
Proteômica
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Química Click
Reação de Cicloadição
Inibidores Enzimáticos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enzyme Inhibitors); 0 (Lactones); 95M8R751W8 (orlistat); EC 3.1.- (Esterases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:27778279
[Au] Autor:Zweerink S; Pollmann T; Ninck S; Kaschani F; Kaiser M
[Ad] Endereço:ZMB, Faculty of Biology, University of Duisburg-Essen, Universitätsstr. 2, 45117, Essen, Germany.
[Ti] Título:Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates.
[So] Source:Methods Mol Biol;1491:23-46, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bioreactive natural products represent versatile starting points for the development of structurally unique activity-based probes. In the present protocol, we describe the workflow for an activity-based protein profiling (ABPP) experiment with an alkyne-tagged natural product derivative. Our protocol includes experimental procedures for in vivo labeling, sample preparation and 2-step (click chemistry) visualization and sample preparation for mass spectrometry-based target identification.
[Mh] Termos MeSH primário: Produtos Biológicos/química
Sondas Moleculares/química
Proteínas/química
[Mh] Termos MeSH secundário: Química Click
Células Hep G2
Seres Humanos
Proteoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Molecular Probes); 0 (Proteins); 0 (Proteome)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:27770631
[Au] Autor:Guo J; Kim GB; Shan D; Kim JP; Hu J; Wang W; Hamad FG; Qian G; Rizk EB; Yang J
[Ad] Endereço:Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
[Ti] Título:Click chemistry improved wet adhesion strength of mussel-inspired citrate-based antimicrobial bioadhesives.
[So] Source:Biomaterials;112:275-286, 2017 01.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:For the first time, a convenient copper-catalyzed azide-alkyne cycloaddition (CuAAC, click chemistry) was successfully introduced into injectable citrate-based mussel-inspired bioadhesives (iCMBAs, iCs) to improve both cohesive and wet adhesive strengths and elongate the degradation time, providing numerous advantages in surgical applications. The major challenge in developing such adhesives was the mutual inhibition effect between the oxidant used for crosslinking catechol groups and the Cu(II) reductant used for CuAAC, which was successfully minimized by adding a biocompatible buffering agent typically used in cell culture, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), as a copper chelating agent. Among the investigated formulations, the highest adhesion strength achieved (223.11 ± 15.94 kPa) was around 13 times higher than that of a commercially available fibrin glue (15.4 ± 2.8 kPa). In addition, dual-crosslinked (i.e. click crosslinking and mussel-inspired crosslinking) iCMBAs still preserved considerable antibacterial and antifungal capabilities that are beneficial for the bioadhesives used as hemostatic adhesives or sealants for wound management.
[Mh] Termos MeSH primário: Adesivos/administração & dosagem
Anti-Infecciosos/administração & dosagem
Fenômenos Fisiológicos Bacterianos/efeitos dos fármacos
Materiais Biomiméticos/síntese química
Bivalves/química
Ácido Cítrico/administração & dosagem
Ácido Cítrico/síntese química
[Mh] Termos MeSH secundário: Adesividade
Adesivos/química
Animais
Anti-Infecciosos/síntese química
Materiais Biomiméticos/administração & dosagem
Química Click/métodos
Desenho de Drogas
Teste de Materiais
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Adhesives); 0 (Anti-Infective Agents); 2968PHW8QP (Citric Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180106
[Lr] Data última revisão:
180106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:28743396
[Au] Autor:Finetti C; Sola L; Elliott J; Chiari M
[Ad] Endereço:National Research Council of Italy, Institute of Chemistry of Molecular Recognition, Via Mario Bianco 9 20131 Milan, Italy.
[Ti] Título:Synthesis of hydrogel via click chemistry for DNA electrophoresis.
[So] Source:J Chromatogr A;1513:226-234, 2017 Sep 01.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This work introduces a novel sieving gel for DNA electrophoresis using a classical click chemistry reaction, the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), to cross-link functional polymer chains. The efficiency of this reaction provides, under mild conditions, hydrogels with near-ideal network connectivity and improved physical properties. Hydrogel formation via click chemistry condensation of functional polymers does not involve the use of toxic monomers and UV initiation. The performance of the new hydrogel in the separation of double stranded DNA fragments was evaluated in the 2200 TapeStation system, an analytical platform, recently introduced by Agilent that combines the advantages of CE in terms of miniaturization and automation with the simplicity of use of slab gel electrophoresis. The click gel enables addition of florescent dyes prior to electrophoresis with considerable improvement of resolution and separation efficiency over conventional cross-linked polyacrylamide gels.
[Mh] Termos MeSH primário: Química Click/métodos
DNA/análise
Eletroforese/métodos
Hidrogel de Polietilenoglicol-Dimetacrilato/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Alquinos/química
Catálise
Polietilenoglicóis/química
Sefarose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Alkynes); 0 (polyacrylamide gels); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); 30IQX730WE (Polyethylene Glycols); 9007-49-2 (DNA); 9012-36-6 (Sepharose)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE



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