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[PMID]: 29458687 [Au] Autor: Tracz DM; Tober AD; Antonation KS; Corbett CR [Ad] Endereço: 1​National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3R2, Canada. [Ti] Título: MALDI-TOF mass spectrometry and high-consequence bacteria: safety and stability of biothreat bacterial sample testing in clinical diagnostic laboratories. [So] Source: J Med Microbiol;67(3):341-346, 2018 Mar. [Is] ISSN: 1473-5644 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: We considered the application of MALDI-TOF mass spectrometry for BSL-3 bacterial diagnostics, with a focus on the biosafety of live-culture direct-colony testing and the stability of stored extracts. Biosafety level 2 (BSL-2) bacterial species were used as surrogates for BSL-3 high-consequence pathogens in all live-culture MALDI-TOF experiments. Viable BSL-2 bacteria were isolated from MALDI-TOF mass spectrometry target plates after 'direct-colony' and 'on-plate' extraction testing, suggesting that the matrix chemicals alone cannot be considered sufficient to inactivate bacterial culture and spores in all samples. Sampling of the instrument interior after direct-colony analysis did not recover viable organisms, suggesting that any potential risks to the laboratory technician are associated with preparation of the MALDI-TOF target plate before or after testing. Secondly, a long-term stability study (3 years) of stored MALDI-TOF extracts showed that match scores can decrease below the threshold for reliable species identification (<1.7), which has implications for proficiency test panel item storage and distribution. [Mh] Termos MeSH primário: Infecções Bacterianas/diagnóstico Técnicas Bacteriológicas Armas Biológicas Técnicas de Laboratório Clínico/métodos Contenção de Riscos Biológicos Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos [Mh] Termos MeSH secundário: Bactérias/isolamento & purificação Infecções Bacterianas/microbiologia Técnicas Bacteriológicas/instrumentação Técnicas de Laboratório Clínico/instrumentação Seres Humanos Manejo de Espécimes/efeitos adversos Manejo de Espécimes/instrumentação Manejo de Espécimes/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biological Warfare Agents) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180308 [Lr] Data última revisão: 180308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180221 [St] Status: MEDLINE [do] DOI: 10.1099/jmm.0.000695

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[PMID]: 28748948 [Au] Autor: Callaway E [Ti] Título: US defence agencies grapple with gene drives. [So] Source: Nature;547(7664):388-389, 2017 07 21. [Is] ISSN: 1476-4687 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Contenção de Riscos Biológicos Tecnologia de Impulso Genético/efeitos adversos Tecnologia de Impulso Genético/métodos Medidas de Segurança [Mh] Termos MeSH secundário: Animais Derramamento de Material Biológico/prevenção & controle Sistemas CRISPR-Cas/genética Culicidae/genética Culicidae/parasitologia Drosophila melanogaster/genética Ecossistema Extinção Biológica Tecnologia de Impulso Genético/legislação & jurisprudência Edição de Genes/legislação & jurisprudência Malária/parasitologia Malária/transmissão Ciência Militar/métodos Medição de Risco Estados Unidos [Pt] Tipo de publicação: NEWS [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170728 [St] Status: MEDLINE [do] DOI: 10.1038/nature.2017.22345

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[PMID]: 28643508 [Ti] Título: Global polio eradication: progress towards containment of poliovirus type 2, worldwide 2017. [Ti] Título: Éradication mondiale de la poliomyélite: progrès réalisés dans le confinement des poliovirus de type 2 à l'échelle mondiale, 2017.. [So] Source: Wkly Epidemiol Rec;92(25):350-6, 2017 06 23. [Is] ISSN: 0049-8114 [Cp] País de publicação: Switzerland [La] Idioma: eng; fre [Mh] Termos MeSH primário: Erradicação de Doenças/organização & administração Saúde Global Poliomielite/prevenção & controle Poliomielite/virologia Vacinas contra Poliovirus/normas Poliovirus [Mh] Termos MeSH secundário: Contenção de Riscos Biológicos/normas Erradicação de Doenças/tendências Seres Humanos Laboratórios/normas Poliovirus/isolamento & purificação Vacina Antipólio Oral/efeitos adversos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Poliovirus Vaccine, Oral); 0 (Poliovirus Vaccines) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170626 [Lr] Data última revisão: 170626 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170624 [St] Status: MEDLINE

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[PMID]: 28628619 [Au] Autor: Paweska JT; Jansen van Vuren P; Meier GH; le Roux C; Conteh OS; Kemp A; Fourie C; Naidoo P; Naicker S; Ohaebosim P; Storm N; Hellferscee O; Ming Sun LK; Mogodi B; Prabdial-Sing N; du Plessis D; Greyling D; Loubser S; Goosen M; McCulloch SD; Scott TP; Moerdyk A; Dlamini W; Konneh K; Kamara IL; Sowa D; Sorie S; Kargbo B; Madhi SA [Ad] Endereço: National Institute for Communicable Diseases, National Health Laboratory Service, Sandringham, South Africa. [Ti] Título: South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory. [So] Source: PLoS Negl Trop Dis;11(6):e0005665, 2017 Jun. [Is] ISSN: 1935-2735 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. METHODS AND FINDINGS: The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone. Furthermore, the NICD set out to establish local capacity amongst Sierra Leonean nationals in all aspects of the FEDL functions from the outset. This led to the successful hand-over of the FEDL to the Sierra Leone Ministry of Health and Sanitation in March 2015. Between 25 August 2014 and 22 June 2016, the laboratory tested 11,250 specimens mostly from the Western Urban and Western Rural regions of Sierra Leone, of which 2,379 (21.14%) tested positive for Ebola virus RNA. CONCLUSIONS: The bio-safety standards and the portability of the SA FEDL, offered a cost-effective and practical alternative for the rapid deployment of a field-operated high biocontainment facility. The SA FEDL teams demonstrated that it is highly beneficial to train the national staff in the course of formidable disease outbreak and accomplished their full integration into all operational and diagnostic aspects of the laboratory. This initiative contributed to the international efforts in bringing the EVD outbreak under control in Sierra Leone, as well as capacitating local African scientists and technologists to respond to diagnostic needs that might be required in future outbreaks of highly contagious pathogens. [Mh] Termos MeSH primário: Contenção de Riscos Biológicos/métodos Testes Diagnósticos de Rotina/métodos Doença pelo Vírus Ebola/diagnóstico Laboratórios/organização & administração [Mh] Termos MeSH secundário: Doença pelo Vírus Ebola/epidemiologia Seres Humanos Cooperação Internacional Serra Leoa/epidemiologia África do Sul [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170720 [Lr] Data última revisão: 170720 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170620 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0005665

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[PMID]: 28505171 [Au] Autor: Zhang Y; Gong Y; Wang C; Liu W; Wang Z; Xia Z; Bu Z; Lu H; Sun Y; Zhang X; Cao Y; Yang F; Su H; Hu Y; Deng Y; Zhou B; Zhao Z; Fu Y; Kargbo D; Dafae F; Kargbo B; Kanu A; Liu L; Qian J; Guo Z [Ad] Endereço: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Changchun, Jilin, China. [Ti] Título: Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic. [So] Source: PLoS Negl Trop Dis;11(5):e0005622, 2017 May. [Is] ISSN: 1935-2735 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: BACKGROUND: Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. METHODOLOGY/PRINCIPAL FINDINGS: On September 26, 2014, China dispatched a Mobile Biosafety Level-3 Laboratory (MBSL-3 Lab) and a well-trained diagnostic team to Sierra Leone to assist in EVD diagnosis using quantitative real-time PCR, which allowed the diagnosis of suspected EVD cases in less than 4 hours from the time of sample receiving. This laboratory was composed of three container vehicles equipped with advanced ventilation system, communication system, electricity and gas supply system. We strictly applied multiple safety precautions to reduce exposure risks. Personnel, materials, water and air flow management were the key elements of the biosafety measures in the MBSL-3 Lab. Air samples were regularly collected from the MBSL-3 Lab, but no evidence of Ebola virus infectious aerosols was detected. Potentially contaminated objects were also tested by collecting swabs. On one occasion, a pipette tested positive for EVD. A total of 1,635 suspected EVD cases (824 positive [50.4%]) were tested from September 28 to November 11, 2014, and no member of the diagnostic team was infected with Ebola virus or other pathogens, including Lassa fever. The specimens tested included blood (69.2%) and oral swabs (30.8%) with positivity rates of 54.2% and 41.9%, respectively. The China mobile laboratory was thus instrumental in the EVD outbreak response by providing timely and reliable diagnostics. CONCLUSIONS/SIGNIFICANCE: The MBSL-3 Lab significantly contributed to establishing a suitable laboratory response capacity during the emergence of EVD in Sierra Leone. [Mh] Termos MeSH primário: Contenção de Riscos Biológicos Arquitetura de Instituições de Saúde/normas Doença pelo Vírus Ebola/diagnóstico Laboratórios/normas Segurança/normas [Mh] Termos MeSH secundário: Ebolavirus Epidemias Doença pelo Vírus Ebola/epidemiologia Seres Humanos Laboratórios/organização & administração RNA Viral/análise Serra Leoa/epidemiologia Fluxo de Trabalho [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Viral) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170720 [Lr] Data última revisão: 170720 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170516 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0005622

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[PMID]: 28131867 [Au] Autor: Hughes L; Wilkins K; Goldsmith CS; Smith S; Hudson P; Patel N; Karem K; Damon I; Li Y; Olson VA; Satheshkumar PS [Ad] Endereço: Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. Electronic address: bkz2@cdc.gov. [Ti] Título: A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories. [So] Source: J Virol Methods;243:68-73, 2017 May. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Virus purification in a high-containment setting provides unique challenges due to barrier precautions and operational safety approaches that are not necessary in lower biosafety level (BSL) 2 environments. The need for high risk group pathogen diagnostic assay development, anti-viral research, pathogenesis and vaccine efficacy research necessitates work in BSL-3 and BSL-4 labs with infectious agents. When this work is performed in accordance with BSL-4 practices, modifications are often required in standard protocols. Classical virus purification techniques are difficult to execute in a BSL-3 or BSL-4 laboratory because of the work practices used in these environments. Orthopoxviruses are a family of viruses that, in some cases, requires work in a high-containment laboratory and due to size do not lend themselves to simpler purification methods. Current CDC purification techniques of orthopoxviruses uses 1,1,2-trichlorotrifluoroethane, commonly known as Genetron . Genetron is a chlorofluorocarbon (CFC) that has been shown to be detrimental to the ozone and has been phased out and the limited amount of product makes it no longer a feasible option for poxvirus purification purposes. Here we demonstrate a new Orthopoxvirus purification method that is suitable for high-containment laboratories and produces virus that is not only comparable to previous purification methods, but improves on purity and yield. [Mh] Termos MeSH primário: Orthopoxvirus/isolamento & purificação Virologia/métodos [Mh] Termos MeSH secundário: Animais Contenção de Riscos Biológicos Seres Humanos Laboratórios Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171002 [Lr] Data última revisão: 171002 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170130 [St] Status: MEDLINE

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[PMID]: 27932614 [Au] Autor: Loftis AJ; Quellie S; Chason K; Sumo E; Toukolon M; Otieno Y; Ellerbrok H; Hobbs MM; Hoover D; Dube K; Wohl DA; Fischer WA [Ad] Endereço: Division of Infectious Diseases, University of North Carolina, Chapel Hill. [Ti] Título: Validation of the Cepheid GeneXpert for Detecting Ebola Virus in Semen. [So] Source: J Infect Dis;215(3):344-350, 2017 Feb 01. [Is] ISSN: 1537-6613 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Background: Ebola virus (EBOV) RNA persistence in semen, reported sexual transmission, and sporadic clusters at the end of the 2013-2016 epidemic have prompted recommendations that male survivors refrain from unprotected sex unless their semen is confirmed to be EBOV free. However, there is no fully validated assay for EBOV detection in fluids other than blood. Methods: The Cepheid Xpert Ebola assay for EBOV RNA detection was validated for whole semen and blood using samples obtained from uninfected donors and spiked with inactivated EBOV. The validation procedure incorporated standards from Clinical and Laboratory Standards Institute and Good Clinical Laboratory Practices guidelines for evaluating molecular devices for use in infectious disease testing. Results: The assay produced limits of detection of 1000 copies/mL in semen and 275 copies/mL in blood. Limits of detection for both semen and blood increased with longer intervals between collection and testing, with acceptable results obtained up to 72 hours after specimen collection. Conclusions: The Cepheid Xpert Ebola assay is accurate and precise for detecting EBOV in whole semen. A validated assay for EBOV RNA detection in semen informs the care of male survivors of Ebola, as well as recommendations for public health. [Mh] Termos MeSH primário: Ebolavirus/isolamento & purificação Reação em Cadeia da Polimerase/métodos RNA Viral/análise Sêmen/virologia [Mh] Termos MeSH secundário: Contenção de Riscos Biológicos Ebolavirus/genética Doença pelo Vírus Ebola/sangue Doença pelo Vírus Ebola/virologia Seres Humanos Masculino Reprodutibilidade dos Testes [Pt] Tipo de publicação: JOURNAL ARTICLE; VALIDATION STUDIES [Nm] Nome de substância: 0 (RNA, Viral) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170606 [Lr] Data última revisão: 170606 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 161210 [St] Status: MEDLINE [do] DOI: 10.1093/infdis/jiw562

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[PMID]: 27754800 [Au] Autor: Howard J; Murashov V; Schulte P [Ad] Endereço: a National Institute for Occupational Safety and Health , Washington, DC. [Ti] Título: Synthetic biology and occupational risk. [So] Source: J Occup Environ Hyg;14(3):224-236, 2017 03. [Is] ISSN: 1545-9632 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Synthetic biology is an emerging interdisciplinary field of biotechnology that involves applying the principles of engineering and chemical design to biological systems. Biosafety professionals have done an excellent job in addressing research laboratory safety as synthetic biology and gene editing have emerged from the larger field of biotechnology. Despite these efforts, risks posed by synthetic biology are of increasing concern as research procedures scale up to industrial processes in the larger bioeconomy. A greater number and variety of workers will be exposed to commercial synthetic biology risks in the future, including risks to a variety of workers from the use of lentiviral vectors as gene transfer devices. There is a need to review and enhance current protection measures in the field of synthetic biology, whether in experimental laboratories where new advances are being researched, in health care settings where treatments using viral vectors as gene delivery systems are increasingly being used, or in the industrial bioeconomy. Enhanced worker protection measures should include increased injury and illness surveillance of the synthetic biology workforce; proactive risk assessment and management of synthetic biology products; research on the relative effectiveness of extrinsic and intrinsic biocontainment methods; specific safety guidance for synthetic biology industrial processes; determination of appropriate medical mitigation measures for lentiviral vector exposure incidents; and greater awareness and involvement in synthetic biology safety by the general occupational safety and health community as well as by government occupational safety and health research and regulatory agencies. [Mh] Termos MeSH primário: Contenção de Riscos Biológicos/métodos Exposição Ocupacional/prevenção & controle Saúde do Trabalhador/normas Biologia Sintética/métodos [Mh] Termos MeSH secundário: Técnicas de Transferência de Genes/normas Vetores Genéticos Seres Humanos Lentivirus/genética Medição de Risco Segurança/normas [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171120 [Lr] Data última revisão: 171120 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161019 [St] Status: MEDLINE [do] DOI: 10.1080/15459624.2016.1237031

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[PMID]: 27404371 [Au] Autor: Nisii C; Vincenti D; Fusco FM; Schmidt-Chanasit J; Carbonnelle C; Raoul H; Eickmann M; Hewson R; Brave A; Nuncio S; Sanchez-Seco MP; Palyi B; Kis Z; Zange S; Nitsche A; Kurth A; Strasser M; Capobianchi MR; Ozin A; Guglielmetti P; Menel-Lemos C; Jacob D; Grunow R; Ippolito G; Di Caro A [Ad] Endereço: 'Lazzaro Spallanzani' National Institute for Infectious Diseases-IRCCS, Rome, Italy. [Ti] Título: The contribution of the European high containment laboratories during the 2014-2015 Ebola Virus Disease emergency. [So] Source: Clin Microbiol Infect;23(2):58-60, 2017 Feb. [Is] ISSN: 1469-0691 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Contenção de Riscos Biológicos Doença pelo Vírus Ebola/epidemiologia Laboratórios [Mh] Termos MeSH secundário: Contenção de Riscos Biológicos/métodos Contenção de Riscos Biológicos/normas Emergências Europa (Continente) Doença pelo Vírus Ebola/diagnóstico Doença pelo Vírus Ebola/história Doença pelo Vírus Ebola/virologia História do Século XXI Seres Humanos Laboratórios/normas Microbiologia [Pt] Tipo de publicação: HISTORICAL ARTICLE; LETTER [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160713 [St] Status: MEDLINE

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[PMID]: 27903826 [Au] Autor: Torres L; Krüger A; Csibra E; Gianni E; Pinheiro VB [Ad] Endereço: Department of Structural and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, U.K. leticia.torres@ucl.ac.uk v.pinheiro@ucl.ac.uk. [Ti] Título: Synthetic biology approaches to biological containment: pre-emptively tackling potential risks. [So] Source: Essays Biochem;60(4):393-410, 2016 Nov 30. [Is] ISSN: 1744-1358 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Biocontainment comprises any strategy applied to ensure that harmful organisms are confined to controlled laboratory conditions and not allowed to escape into the environment. Genetically engineered microorganisms (GEMs), regardless of the nature of the modification and how it was established, have potential human or ecological impact if accidentally leaked or voluntarily released into a natural setting. Although all evidence to date is that GEMs are unable to compete in the environment, the power of synthetic biology to rewrite life requires a pre-emptive strategy to tackle possible unknown risks. Physical containment barriers have proven effective but a number of strategies have been developed to further strengthen biocontainment. Research on complex genetic circuits, lethal genes, alternative nucleic acids, genome recoding and synthetic auxotrophies aim to design more effective routes towards biocontainment. Here, we describe recent advances in synthetic biology that contribute to the ongoing efforts to develop new and improved genetic, semantic, metabolic and mechanistic plans for the containment of GEMs. [Mh] Termos MeSH primário: Contenção de Riscos Biológicos/métodos Biologia Sintética/métodos [Mh] Termos MeSH secundário: Engenharia Genética Genoma Plasmídeos/metabolismo Risco [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170619 [Lr] Data última revisão: 170619 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161202 [St] Status: MEDLINE

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