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  1 / 10906 MEDLINE  
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[PMID]:29377949
[Au] Autor:Hibino A; Saito R; Taniguchi K; Zaraket H; Shobugawa Y; Matsui T; Suzuki H; Japanese HRSV Collaborative Study Group
[Ad] Endereço:Division of International Health (Public Health), Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
[Ti] Título:Molecular epidemiology of human respiratory syncytial virus among children in Japan during three seasons and hospitalization risk of genotype ON1.
[So] Source:PLoS One;13(1):e0192085, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the genetic diversity, the circulation patterns, and risk for hospital admission of human respiratory syncytial virus (HRSV) strains in Japan between 2012 through 2015. During the study period, 744 HRSV-positive cases were identified by rapid diagnostic test. Of these, 572 samples were positive by real-time PCR; 400 (69.9%) were HRSV-A, and 172 (30.1%) were HRSV-B. HRSV-A and -B alternated as the dominant strain in the subsequent seasons. Phylogenetic tree analysis of the second hyper-variable region of the G protein classified the HRSV-A specimens into NA1 (n = 242) and ON1 (n = 114) genotypes and the HRSV-B specimens into BA9 (n = 60), and BA10 (n = 27). The ON1 genotype, containing a 72-nucleotide duplication in the G protein's second hyper-variable region, was first detected in the 2012-2013 season but it predominated and replaced the older NA1 HRSV-A in the 2014-2015 season, which also coincided with a record number of HRSV cases reported to the National Infectious Disease Surveillance in Japan. The risk of hospitalization was 6.9 times higher for the ON1 genotype compared to NA1. In conclusion, our data showed that the emergence and predominance of the relatively new ON1 genotype in Japan was associated with a record high number of cases and increased risk for hospitalization.
[Mh] Termos MeSH primário: Hospitalização
Epidemiologia Molecular
Vírus Sincicial Respiratório Humano/genética
[Mh] Termos MeSH secundário: Genótipo
Seres Humanos
Japão
Reação em Cadeia da Polimerase em Tempo Real
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192085


  2 / 10906 MEDLINE  
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[PMID]:29458550
[Au] Autor:Petridou E; Jensen CB; Arvanitidis A; Giannaki-Psinaki M; Michos A; Krogfelt KA; Petersen RF
[Ad] Endereço:1​Department of Clinical Microbiology, 'Aghia Sophia' Children's Hospital, Athens, Greece.
[Ti] Título:Molecular epidemiology of Bordetella pertussis in Greece, 2010-2015.
[So] Source:J Med Microbiol;67(3):400-407, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY: Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 real-time PCR confirmed Bordetella spp./B. pertussis infection in 300 samples. A subset of samples (n=153) were analysed by multi-locus variable number tandem repeat analysis (MLVA) and (n=25) by sequence-based typing of the toxin promotor region (ptxP) on DNA extracted from clinical specimens.Results/Key findings. A complete MLVA profile was determined in 66 out of 153 samples; the B. pertussis MLVA type 27 (n=55) was the dominant genotype and all tested samples (n=25) expressed the ptxP3 genotype. The vaccine coverage in the Greek population was 90 %; however, the study population expressed complete coverage in 2 out of 264 infants (0-11 months) and in 20 out of 36 children (1-14 years). Roma and immigrant minorities represent 7 % of the Greek population, but make up 50 % of the study population, indicating a low vaccine coverage among these groups. CONCLUSIONS: The B. pertussis MT27 and ptxP3 genotype is dominant in Greek, Roma and immigrant infants and children hospitalized in Greece. Thus, the predominant MLVA genotype in Greece is similar to other countries using acellular vaccines.
[Mh] Termos MeSH primário: Bordetella pertussis/genética
Coqueluche/epidemiologia
Coqueluche/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Bordetella pertussis/isolamento & purificação
Criança
Pré-Escolar
DNA Bacteriano/genética
Feminino
Variação Genética
Genótipo
Grécia/epidemiologia
Hospitais Pediátricos
Seres Humanos
Lactente
Recém-Nascido
Masculino
Repetições Minissatélites
Epidemiologia Molecular
Tipagem de Sequências Multilocus
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
Coqueluche/diagnóstico
Coqueluche/etnologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000688


  3 / 10906 MEDLINE  
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[PMID]:29107195
[Au] Autor:Yokoyama E; Hirai S; Ishige T; Murakami S
[Ad] Endereço:Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2 Nitona, Chuo, Chiba City, Chiba 260-8715, Japan. Electronic address: e.ykym@pref.chiba.lg.jp.
[Ti] Título:Application of whole genome sequence data in analyzing the molecular epidemiology of Shiga toxin-producing Escherichia coli O157:H7/H.
[So] Source:Int J Food Microbiol;264:39-45, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Seventeen clusters of Shiga toxin-producing Escherichia coli O157:H7/- (O157) strains, determined by cluster analysis of pulsed-field gel electrophoresis patterns, were analyzed using whole genome sequence (WGS) data to investigate this pathogen's molecular epidemiology. The 17 clusters included 136 strains containing strains from nine outbreaks, with each outbreak caused by a single source contaminated with the organism, as shown by epidemiological contact surveys. WGS data of these strains were used to identify single nucleotide polymorphisms (SNPs) by two methods: short read data were directly mapped to a reference genome (mapping derived SNPs) and common SNPs between the mapping derived SNPs and SNPs in assembled data of short read data (common SNPs). Among both SNPs, those that were detected in genes with a gap were excluded to remove ambiguous SNPs from further analysis. The effectiveness of both SNPs was investigated among all the concatenated SNPs that were detected (whole SNP set); SNPs were divided into three categories based on the genes in which they were located (i.e., backbone SNP set, O-island SNP set, and mobile element SNP set); and SNPs in non-coding regions (intergenic region SNP set). When SNPs from strains isolated from the nine single source derived outbreaks were analyzed using an unweighted pair group method with arithmetic mean tree (UPGMA) and a minimum spanning tree (MST), the maximum pair-wise distances of the backbone SNP set of the mapping derived SNPs were significantly smaller than those of the whole and intergenic region SNP set on both UPGMAs and MSTs. This significant difference was also observed when the backbone SNP set of the common SNPs were examined (Steel-Dwass test, P≤0.01). When the maximum pair-wise distances were compared between the mapping derived and common SNPs, significant differences were observed in those of the whole, mobile element, and intergenic region SNP set (Wilcoxon signed rank test, P≤0.01). When all the strains included in one complex on an MST or one cluster on a UPGMA were designated as the same genotype, the values of the Hunter-Gaston Discriminatory Power Index for the backbone SNP set of the mapping derived and common SNPs were higher than those of other SNP sets. In contrast, the mobile element SNP set could not robustly subdivide lineage I strains of tested O157 strains using both the mapping derived and common SNPs. These results suggested that the backbone SNP set were the most effective for analysis of WGS data for O157 in enabling an appropriation of its molecular epidemiology.
[Mh] Termos MeSH primário: DNA Intergênico/genética
Infecções por Escherichia coli/epidemiologia
Escherichia coli O157/genética
Genoma Bacteriano/genética
Polimorfismo de Nucleotídeo Único/genética
[Mh] Termos MeSH secundário: Surtos de Doenças
Eletroforese em Gel de Campo Pulsado
Infecções por Escherichia coli/microbiologia
Escherichia coli O157/isolamento & purificação
Escherichia coli O157/patogenicidade
Genótipo
Seres Humanos
Epidemiologia Molecular
Toxina Shiga/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Intergenic); 75757-64-1 (Shiga Toxin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171107
[St] Status:MEDLINE


  4 / 10906 MEDLINE  
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[PMID]:28454556
[Au] Autor:Daw MA; El-Bouzedi A; Ahmed MO; Dau AA; In association with the Libyan Study Group of Hepatitis & HIV
[Ad] Endereço:Department of Medical Microbiology, Faculty of Medicine, University of Tripoli, CC 82668, Tripoli, Libya. mohamedadaw@gmail.com.
[Ti] Título:Molecular and epidemiological characterization of HIV-1 subtypes among Libyan patients.
[So] Source:BMC Res Notes;10(1):170, 2017 Apr 28.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The epidemiological and clinical aspects of human immunodeficiency virus subtypes are of great interest worldwide. These subtypes are rarely studied in North African countries. Libya is a large country with the longest coast on the Mediterranean Sea, facing the Southern European countries. Studies on the characterization of HIV-1 subtypes are limited in Libya. This study aimed to determine the magnitude of the HIV problem among the Libyan population and to better understand the genetic diversity and the epidemiologic dynamics of HIV 1, as well as to correlate that with the risk factors involved. METHODS: A total of 159 HIV-1 strains were collected from 814 HIV positive patients from the four Libyan regions during a 16-year period (1995-2010). To determine the HIV-1 subtypes, genetic analysis and molecular sequencing were carried out using provirus polygene. Epidemiologic and demographic information was obtained from each participant and correlated with HIV-1 subtypes using logistic regression. RESULTS: The overall prevalence of HIV among Libyans ranged from 5 to 10 per 100,000 during the study period. It was higher among intravenous drug users (IVDUs) (53.9%), blood recipients (25.9%) and heterosexuals (17.6%) than by vertical transmission (2.6%). Prevalence was higher among males aged 20-40 years (M:F 1:6, P > 0.001). Among the 159 strains of HIV-1 available for typing, 117 strains (73.6%) were subtype B, 29 (18.2%) were CRF02_AG, and 13 (8.2%) were subtype A. HIV-1 subtype B was the most prevalent all over the country, and it was more prevalent in the Northern region, particularly among IVDUs (P < 0.001). GRF02_AG was common in the Eastern region, particularly among blood recipients while subtype A emerged in the Western region, particularly among IVDUs. CONCLUSIONS: HIV-1 infection is emerging in Libya with a shifting prevalence of subtypes associated with the changing epidemiology of HIV-1 among risk groups. A genetic analysis of HIV-1 strains demonstrated low subtype heterogeneity with the evolution of subtype B, and CRF_20 AG, as well as HIV-1 subtype A. Our study highlights the importance of expanded surveillance programs to control HIV infection and the necessity of introducing public health strategies to target the risk groups, particularly IVDUs.
[Mh] Termos MeSH primário: Infecções por HIV/epidemiologia
Infecções por HIV/transmissão
HIV-1/genética
Transmissão Vertical de Doença Infecciosa/estatística & dados numéricos
Filogenia
Abuso de Substâncias por Via Intravenosa/epidemiologia
[Mh] Termos MeSH secundário: Adulto
Transfusão de Sangue
Feminino
Infecções por HIV/complicações
Infecções por HIV/virologia
HIV-1/classificação
HIV-1/isolamento & purificação
Seres Humanos
Líbia/epidemiologia
Masculino
Meia-Idade
Epidemiologia Molecular
Tipagem Molecular
Prevalência
Vigilância em Saúde Pública
Fatores de Risco
Fatores Sexuais
Abuso de Substâncias por Via Intravenosa/complicações
Abuso de Substâncias por Via Intravenosa/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-017-2491-2


  5 / 10906 MEDLINE  
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[PMID]:29224130
[Au] Autor:Wang M; Wang Y; Baloch AR; Pan Y; Xu F; Tian L; Zeng Q
[Ad] Endereço:College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
[Ti] Título:Molecular epidemiology and characterization of bovine leukemia virus in domestic yaks (Bos grunniens) on the Qinghai-Tibet Plateau, China.
[So] Source:Arch Virol;163(3):659-670, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine leukemia virus (BLV) is a member of the genus Deltaretrovirus of the family Retroviridae and cause a chronic lymphosarcoma, which is extensive in cattle. In yaks (Bos grunniens), the distribution, strains and genetic characteristics of BLV have rarely been studied. The aim of our study was to investigate BLV infections in domestic yaks and determine the genetic variability of BLV circulating in a region of the Qinghai Tibet Plateau, China. Blood samples were collected from 798 yaks, which were from different farms from Gansu, Qinghai and Sichuan provinces surrounding the Qinghai-Tibet Plateau. Nested PCR targeting BLV long terminal repeats was used to detect the BLV provirus. The highest prevalence of BLV infection was in Gansu province, where it was 18.93% (39/206) in white yaks from Tianzhu City and 19.14% (31/162) in black yaks from Gannan City. In Qinghai and Sichuan provinces, the prevalence of BLV in black yaks was 14.83% (35/236) and 14.94% (29/194), respectively. The prevalence of BLV was not significantly different in yaks up to one year old than in older animals. Phylogenetic analysis was performed using 16 different env-gp51 (497-bp) gene sequences from the three provinces and 71 known BLV strains, which revealed that in both Gansu and Qinghai provinces, genotypes 6 and 10 of the BLV strains were at high levels, whereas only genotype 10 was prevalent in Sichuan Province. Phylogenetic analysis and sequence comparisons revealed 95.7-99.8% sequence identity among the full-length env genes of 16 strains, nearly full-length genome sequences of six BLV strains, and those of the known genotypes 6 and 10 of BLV. This study provides comprehensive information is regarding the widespread infection of domestic yaks with BLV on the Qinghai-Tibet Plateau of China, and shows that at least two BLV genotypes (genotypes 6 and 10) are circulating in this population.
[Mh] Termos MeSH primário: Leucose Enzoótica Bovina/epidemiologia
Genes env
Genótipo
Vírus da Leucemia Bovina/classificação
Vírus da Leucemia Bovina/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bovinos
Leucose Enzoótica Bovina/transmissão
Leucose Enzoótica Bovina/virologia
Expressão Gênica
Vírus da Leucemia Bovina/isolamento & purificação
Epidemiologia Molecular
Prevalência
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Sequências Repetidas Terminais
Tibet/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3658-9


  6 / 10906 MEDLINE  
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[PMID]:28456705
[Au] Autor:Li QJ; Jiao WW; Yin QQ; Li YJ; Li JQ; Xu F; Sun L; Xiao J; Qi H; Wang T; Mokrousov I; Huang HR; Shen AD
[Ad] Endereço:Ministry of Education Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute
[Ti] Título:Positive epistasis of major low-cost drug resistance mutations rpoB531-TTG and katG315-ACC depends on the phylogenetic background of Mycobacterium tuberculosis strains.
[So] Source:Int J Antimicrob Agents;49(6):757-762, 2017 Jun.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycobacterium tuberculosis Beijing genotype strains increasingly circulate in different world regions, either as historical endemic, e.g. in East Asia, or recently imported, e.g. in South America, and this family is regarded as the most successful lineage of the global tuberculosis (TB) epidemic. Here we analysed the transmission capacity of these strains in the context of their phylogenetic background and drug resistance mutations. The study collection included all multidrug resistant (MDR) strains of Beijing genotype isolated in Beijing Chest Hospital, the largest tertiary TB facility in North China, in 2011-2013 (n = 278). Strains were subjected to NTF/IS6110 and 24-loci MIRU-VNTR analysis. Drug resistance mutations were detected in rpoB, katG, inhA and oxyR-ahpC. A total of 58 and 220 strains were assigned to the ancient and modern Beijing sublineages, respectively. 24-MIRU-VNTR clustering was higher in modern versus ancient Beijing strains (35.9% vs. 12.1%; P <0.001). After taking into consideration the presence of rpoB and katG mutations, clustering decreased to 15.9% in modern and 0% in ancient strains. The most frequent combination of mutations (rpoB531-TTG and katG315-ACC) was more prevalent in clustered versus non-clustered isolates in the modern sublineage (23/35 vs. 47/185; P <0.0001). To conclude, a combination of the known low-fitness-cost rpoB531-TTG and katG315-ACC mutations likely facilitates the increased transmission ability of MDR strains of the modern but not ancient Beijing sublineage. Accordingly, positive epistasis of major low-cost drug resistance-conferring mutations is influenced by the phylogenetic background of M. tuberculosis strains.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Farmacorresistência Bacteriana Múltipla
Mutação
Mycobacterium tuberculosis/classificação
Mycobacterium tuberculosis/efeitos dos fármacos
Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia
Tuberculose Resistente a Múltiplos Medicamentos/transmissão
[Mh] Termos MeSH secundário: Adulto
Idoso
China/epidemiologia
Análise por Conglomerados
Transmissão de Doença Infecciosa
Feminino
Seres Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
Mycobacterium tuberculosis/genética
Mycobacterium tuberculosis/isolamento & purificação
Filogenia
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  7 / 10906 MEDLINE  
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[PMID]:27771517
[Au] Autor:Amraoui F; Failloux AB
[Ad] Endereço:Institut Pasteur, Department of Virology, Arboviruses and Insect Vectors, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
[Ti] Título:Chikungunya: an unexpected emergence in Europe.
[So] Source:Curr Opin Virol;21:146-150, 2016 12.
[Is] ISSN:1879-6265
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Since the first outbreak of chikungunya in Italy in 2007, Europe has been facing an increase in local transmission of arboviral diseases. Dengue virus (DENV) and chikungunya virus (CHIKV) are both transmitted by the mosquito Aedes albopictus present in 20 European countries. CHIKV emergence in Europe was mainly associated with the East-Central-South African (ECSA) genotype, recently exemplified by the 11 CHIKV cases in southern France in 2014. Despite hundreds of travelers returning from the Americas where the Asian CHIKV genotype was responsible for more than one million cases, no autochthonous transmission associated with the Asian genotype was reported in Europe. Thus the pattern of transmission can be significantly different depending on the mosquito population, the virus genotype and environmental factors such as temperature.
[Mh] Termos MeSH primário: Aedes/crescimento & desenvolvimento
Febre de Chikungunya/epidemiologia
Febre de Chikungunya/transmissão
Vírus Chikungunya/isolamento & purificação
Transmissão de Doença Infecciosa
[Mh] Termos MeSH secundário: Animais
Vírus Chikungunya/classificação
Vírus Chikungunya/genética
França/epidemiologia
Itália/epidemiologia
Epidemiologia Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 10906 MEDLINE  
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[PMID]:28469994
[Au] Autor:Li T; Lu H; Wang X; Gao Q; Dai Y; Shang J; Li M
[Ad] Endereço:Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityShanghai, China.
[Ti] Título:Molecular Characteristics of Causing Bovine Mastitis between 2014 and 2015.
[So] Source:Front Cell Infect Microbiol;7:127, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is highly pathogenic and can cause diseases in both humans and domestic animals. In animal species, including ruminants, may cause severe or sub-clinical mastitis. This study aimed to investigate the molecular profile, antimicrobial resistance, and genotype/phenotype correlation of 212 isolates recovered from cases of bovine mastitis from 2014 to 2015 in the Shanghai and Zhejiang areas of China. Nineteen sequence types (STs) were determined by multi-locus sequence typing, while the dominant ST was ST97, followed by ST520, ST188, ST398, ST7, and ST9. Within 14 methicillin-resistant (MRSA) isolates and 198 methicillin-susceptible (MSSA) isolates, ST97 was the predominant MSSA clone and ST9-MRSA-SCCmecXII-spa t899 was the most common MRSA clone. The MRSA strains showed much higher rates of resistance to multiple antibiotics than did MSSA strains. Compared with other MSSA strains, MSSA ST398 was more resistant to clindamycin, erythromycin, and ciprofloxacin. No isolates were resistant to vancomycin, teicoplanin, or linezolid. The molecular profiles of the virulence genes varied in different strains. ST520 strains carried seg-sei-sem-sen-seo genes, and ST9 and ST97 harbored sdrD-sdrE genes. Virulence phenotype analysis showed diversity in different clones. Biofilm formation ability was significantly enhanced in ST188 and ST7, and red blood cell lysis capacity was relatively strong in all strains of animal origin except ST7. Our results indicate that MSSA was the predominant strain causing bovine mastitis in eastern regions of China. However, the presence of multidrug resistant and toxigenic MRSA clone ST9 suggests that comprehensive surveillance of infection should be implemented in the management of animal husbandry products.
[Mh] Termos MeSH primário: Mastite Bovina/epidemiologia
Mastite Bovina/microbiologia
Epidemiologia Molecular
Infecções Estafilocócicas/epidemiologia
Staphylococcus aureus/genética
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Antígenos de Bactérias/genética
Técnicas de Tipagem Bacteriana
Biofilmes/crescimento & desenvolvimento
Bovinos
China/epidemiologia
DNA Bacteriano
Farmacorresistência Bacteriana Múltipla/genética
Feminino
Genótipo
Resistência a Meticilina
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/patogenicidade
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus/métodos
Proteínas de Ligação às Penicilinas/genética
Fenótipo
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/classificação
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/isolamento & purificação
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antigens, Bacterial); 0 (DNA, Bacterial); 0 (Penicillin-Binding Proteins); 0 (Virulence Factors); 0 (streptococcal protective antigen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00127


  9 / 10906 MEDLINE  
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[PMID]:29254479
[Au] Autor:Sekizuka T; Nai E; Yoshida T; Endo S; Hamajima E; Akiyama S; Ikuta Y; Obana N; Kawaguchi T; Hayashi K; Noda M; Sumita T; Kokaji M; Katori T; Hashino M; Oba K; Kuroda M
[Ad] Endereço:Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
[Ti] Título:Streptococcal toxic shock syndrome caused by the dissemination of an invasive emm3/ST15 strain of Streptococcus pyogenes.
[So] Source:BMC Infect Dis;17(1):774, 2017 12 18.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Streptococcus pyogenes (group A Streptococcus [GAS]) is a major human pathogen that causes a wide spectrum of clinical manifestations. Although invasive GAS (iGAS) infections are relatively uncommon, emm3/ST15 GAS is a highly virulent, invasive, and pathogenic strain. Global molecular epidemiology analysis has suggested that the frequency of emm3 GAS has been recently increasing. CASE PRESENTATION: A 14-year-old patient was diagnosed with streptococcal toxic shock syndrome and severe pneumonia, impaired renal function, and rhabdomyolysis. GAS was isolated from a culture of endotracheal aspirates and designated as KS030. Comparative genome analysis suggested that KS030 is classified as emm3 (emm-type) and ST15 (multilocus sequencing typing [MLST]), which is similar to iGAS isolates identified in the UK (2013) and Switzerland (2015). CONCLUSIONS: We conclude that the global dissemination of emm3/ST15 GAS strain has the potential to cause invasive disease.
[Mh] Termos MeSH primário: Choque Séptico/microbiologia
Infecções Estreptocócicas/microbiologia
Streptococcus pyogenes/isolamento & purificação
[Mh] Termos MeSH secundário: Antígenos de Bactérias/genética
Proteínas da Membrana Bacteriana Externa/genética
Seres Humanos
Epidemiologia Molecular
Tipagem de Sequências Multilocus
Choque Séptico/epidemiologia
Infecções Estreptocócicas/epidemiologia
Streptococcus pyogenes/classificação
Streptococcus pyogenes/genética
Suíça/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Outer Membrane Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2870-2


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Suffys, Philip N
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[PMID]:29244821
[Au] Autor:Fontes ANB; Lima LNGC; Mota RMS; Almeida RLF; Pontes MA; Gonçalves HS; Frota CC; Vissa VD; Brennan PJ; Guimaraes RJPS; Kendall C; Kerr LRFS; Suffys PN
[Ad] Endereço:Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
[Ti] Título:Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil.
[So] Source:PLoS Negl Trop Dis;11(12):e0006117, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leprosy is endemic in large part of Brazil with 28,761 new patients in 2015, the second largest number worldwide and reaches 9/10.000 in highly endemic regions and 2.7/10.000 in the city of Fortaleza, Ceará, Northeast Brazil. For better understanding of risk factors for leprosy transmission, we conducted an epidemiologic study supplemented by 17 locus VNTR and SNP 1-4 typing of Mycobacterium leprae in skin biopsy samples from new multibacillary (MB) patients diagnosed at a reference center in 2009 and 2010. Among the 1,519 new patients detected during the study period, 998 (65.7%) were MB and we performed DNA extraction and genotyping on 160 skin biopsy samples, resulting in 159 (16%) good multilocus VNTR types. Thirty-eight of these patients also provided VNTR types from M. leprae in nasal swabs. The SNP-Type was obtained for 157 patients and 87% were of type 4. Upon consideration all VNTR markers, 156 different genotypes and three pairs with identical genotypes were observed; no epidemiologic relation could be observed between individuals in these pairs. Considerable variability in differentiating index (DI) was observed between the different markers and the four with highest DI [(AT)15, (TA)18, (AT)17 and (GAA)21] frequently demonstrated differences in copy number when comparing genotypes from both type of samples. Excluding these markers from analysis resulted in 83 genotypes, 20 of which included 96 of the patients (60.3%). These clusters were composed of two (n = 8), three (n = 6), four (n = 1), five (n = 2), six (n = 1), 19 (n = 1) and 23 (n = 23) individuals and suggests that recent transmission is contributing to the maintenance of leprosy in Fortaleza. When comparing epidemiological and clinical variables among patients within clustered or with unique M. leprae genotypes, a positive bacterial index in skin biopsies and knowledge of working with someone with the disease were significantly associated with clustering. A tendency to belong to a cluster was observed with later notification of disease (mean value of 3.4 months) and having disability grade 2. A tendency for lack of clustering was observed for patients who reported to have lived with another leprosy case but this might be due to lack of inclusion of household contacts in the study. Although clusters were spread over the city, kernel analysis revealed that some of the patients belonging to the two major clusters were spatially related to some neighborhoods that report poverty and high disease incidence in children. Finally, inclusion of genotypes from nasal swabs might be warranted. A major limitation of the study is that sample size of 160 patients from a two year period represents only 15% of the new patients and this could have weakened statistical outcomes. This is the first molecular epidemiology study of leprosy in Brazil and although the high clustering level suggests that recent transmission is the major cause of disease in Fortaleza; the existence of two large clusters needs further investigation.
[Mh] Termos MeSH primário: Hanseníase/transmissão
Mycobacterium leprae/genética
[Mh] Termos MeSH secundário: Brasil/epidemiologia
Análise por Conglomerados
Genótipo
Geografia
Seres Humanos
Hanseníase/microbiologia
Repetições Minissatélites/genética
Epidemiologia Molecular
Fatores de Risco
Análise Espacial
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006117



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