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[PMID]:29231025
[Au] Autor:Shen X; Li M; Wang YL; Chen YL; Lin Y; Zhao ZM; Que TZ
[Ad] Endereço:Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
[Ti] Título:[Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method].
[So] Source:Fa Yi Xue Za Zhi;33(2):168-170, 2017 Apr.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the forensic application value of MPure-12 automatic nucleic acid purification (MPure-12 Method) for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. METHODS: Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles. RESULTS: The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains (20 µL, 15 µL, 10 µL, 5 µL, 1 µL) showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 µL blood volume by MPure-12 method. CONCLUSIONS: MPure-12 method is suitable for DNA extraction of a certain concentration blood samples.Chelex-100 method may be better for the extraction of trace blood samples.This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.
[Mh] Termos MeSH primário: Quelantes
DNA/isolamento & purificação
Medicina Legal/métodos
Reação em Cadeia da Polimerase/métodos
Poliestirenos
Polivinil
[Mh] Termos MeSH secundário: Alelos
Manchas de Sangue
DNA/sangue
Impressões Digitais de DNA
Genótipo
Seres Humanos
Masculino
Resinas Sintéticas
Saliva
Sêmen/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chelating Agents); 0 (Polystyrenes); 0 (Polyvinyls); 0 (Resins, Synthetic); 11139-85-8 (Chelex 100); 80208-96-4 (chelex); 9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.02.013


  2 / 13755 MEDLINE  
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[PMID]:29205006
[Au] Autor:Zou Y; Guo JJ; Li QP; Zuo DH; Liu JS; Guo YD; Yan J; Zha L; Cai JF; Lan LM
[Ad] Endereço:Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China.
[Ti] Título:Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population.
[So] Source:Fa Yi Xue Za Zhi;32(5):356-362, 2016 Oct.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To investigate the genetic polymorphisms of 21 short tandem repeat (STR) loci ( , , , , , , , , , , , , , , , , , , , and ). METHODS: A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer. RESULTS: A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except ( =0.023). The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10⁻²5, respectively. CONCLUSIONS: The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing.
[Mh] Termos MeSH primário: Grupo com Ancestrais do Continente Asiático/genética
Genética Populacional
Repetições de Microssatélites
Polimorfismo Genético
[Mh] Termos MeSH secundário: Alelos
China
Impressões Digitais de DNA
Frequência do Gene
Testes Genéticos
Genótipo
Seres Humanos
Reação em Cadeia da Polimerase
Probabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.05.010


  3 / 13755 MEDLINE  
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[PMID]:28743451
[Au] Autor:He G; Li Y; Wang Z; Liang W; Luo H; Liao M; Zhang J; Yan J; Li Y; Hou Y; Wu J
[Ad] Endereço:Department of Forensic Genetics, West China School of Preclinical Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
[Ti] Título:Genetic diversity of 21 autosomal STR loci in the Han population from Sichuan province, Southwest China.
[So] Source:Forensic Sci Int Genet;31:e33-e35, 2017 11.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Exploration of the ethnic origin and genetic differentiation of 56 Chinese officially recognized nationalities populations played a fundamental role in the research field of population genetics, forensic science, linguistics, anthropology, and archaeology. In the present study, population data of 21 autosomal STR loci (CSF1PO, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D2S1338, D2S441, D3S1358, D5S818, D6S1043, D7S820, D8S1179, FGA, Penta D, Penta E, TH01, TPOX, and vWA) included in the AGCU EX22 kit in 2793 Southwest Han Chinese individuals was obtained and population genetic relationships among 28 Chinese populations were investigated. Our study indicated that the twenty-one autosomal STRs are highly polymorphic in the Sichuan Han population and can be used as a powerful tool in the routine forensic usage. MDS and phylogenetic analysis suggested that the Sichuan Han population kept a close genetic relationship with the southwest populations.
[Mh] Termos MeSH primário: Grupos Étnicos/genética
Genética Populacional
Repetições de Microssatélites
Polimorfismo Genético
[Mh] Termos MeSH secundário: China
Impressões Digitais de DNA
Frequência do Gene
Seres Humanos
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:28743033
[Au] Autor:Yahya P; Sulong S; Harun A; Wan Isa H; Ab Rajab NS; Wangkumhang P; Wilantho A; Ngamphiw C; Tongsima S; Zilfalil BA
[Ad] Endereço:Department of Paediatric, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, 16150 Kelantan, Malaysia.
[Ti] Título:Analysis of the genetic structure of the Malay population: Ancestry-informative marker SNPs in the Malay of Peninsular Malaysia.
[So] Source:Forensic Sci Int Genet;30:152-159, 2017 09.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Malay, the main ethnic group in Peninsular Malaysia, is represented by various sub-ethnic groups such as Melayu Banjar, Melayu Bugis, Melayu Champa, Melayu Java, Melayu Kedah Melayu Kelantan, Melayu Minang and Melayu Patani. Using data retrieved from the MyHVP (Malaysian Human Variome Project) database, a total of 135 individuals from these sub-ethnic groups were profiled using the Affymetrix GeneChip Mapping Xba 50-K single nucleotide polymorphism (SNP) array to identify SNPs that were ancestry-informative markers (AIMs) for Malays of Peninsular Malaysia. Prior to selecting the AIMs, the genetic structure of Malays was explored with reference to 11 other populations obtained from the Pan-Asian SNP Consortium database using principal component analysis (PCA) and ADMIXTURE. Iterative pruning principal component analysis (ipPCA) was further used to identify sub-groups of Malays. Subsequently, we constructed an AIMs panel for Malays using the informativeness for assignment (I ) of genetic markers, and the K-nearest neighbor classifier (KNN) was used to teach the classification models. A model of 250 SNPs ranked by I , correctly classified Malay individuals with an accuracy of up to 90%. The identified panel of SNPs could be utilized as a panel of AIMs to ascertain the specific ancestry of Malays, which may be useful in disease association studies, biomedical research or forensic investigation purposes.
[Mh] Termos MeSH primário: Grupos Étnicos/genética
Genética Populacional
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Impressões Digitais de DNA
Genótipo
Seres Humanos
Malásia
Análise de Componente Principal
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  5 / 13755 MEDLINE  
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[PMID]:28743032
[Au] Autor:Gouy A; Zieger M
[Ad] Endereço:Computational and Molecular Population Genetics, Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012 Bern, Switzerland; Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland. Electronic address: Alexandre.Gouy@iee.unibe.ch.
[Ti] Título:STRAF-A convenient online tool for STR data evaluation in forensic genetics.
[So] Source:Forensic Sci Int Genet;30:148-151, 2017 09.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Population data in forensic genetics has to be checked for a variety of statistical parameters before it can be employed for case work. A lot of very powerful statistical tools are available for this task, most of them developed by labs having their research focus on population genetics or evolution. However, most of these programs require a substantial amount of experience. In addition, to our knowledge, none of the freely available programs calculates all the common parameters for a population study in forensic genetics at once, based on a single input file. We present here a convenient online tool that fills this gap. STRAF (STR Analysis for Forensics) provides an intuitive interface and input file format and computes all the relevant parameters for a classical population study based on autosomal STR data at once and in a convenient way. In addition, STRAF includes a PCA module that can be used for population substructure detection or quality control. The results generated by the program were verified by recalculating parameters from an already published population study.
[Mh] Termos MeSH primário: Genética Populacional
Internet
Repetições de Microssatélites
Software
[Mh] Termos MeSH secundário: Impressões Digitais de DNA
Frequência do Gene
Variação Genética
Seres Humanos
Desequilíbrio de Ligação
Análise de Componente Principal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  6 / 13755 MEDLINE  
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[PMID]:28463807
[Au] Autor:Phillips C
[Ad] Endereço:Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Galicia, Spain. Electronic address: c.phillips@mac.com.
[Ti] Título:A genomic audit of newly-adopted autosomal STRs for forensic identification.
[So] Source:Forensic Sci Int Genet;29:193-204, 2017 07.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In preparation for the growing use of massively parallel sequencing (MPS) technology to genotype forensic STRs, a comprehensive genomic audit of 73 STRs was made in 2016 [Parson et al., Forensic Sci. Int. Genet. 22, 54-63]. The loci examined included miniSTRs that were not in widespread use, but had been incorporated into MPS kits or were under consideration for this purpose. The current study expands the genomic analysis of autosomal STRs that are not commonly used, to include the full set of developed miniSTRs and an additional 24 STRs, most of which have been recently included in several supplementary forensic multiplex kits for capillary electrophoresis. The genomic audit of these 47 newly-adopted STRs examined the linkage status of new loci on the same chromosome as established forensic STRs; analyzed world-wide population variation of the newly-adopted STRs using published data; assessed their forensic informativeness; and compiled the sequence characteristics, repeat structures and flanking regions of each STR. A further 44 autosomal STRs developed for forensic analyses but not incorporated into commercial kits, are also briefly described.
[Mh] Termos MeSH primário: Impressões Digitais de DNA
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Impressões Digitais de DNA/instrumentação
Ligação Genética
Genoma Humano
Seres Humanos
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  7 / 13755 MEDLINE  
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[PMID]:28461042
[Au] Autor:Wang Z; Du W; He G; Liu J; Hou Y
[Ad] Endereço:Institute of Forensic Medicine, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
[Ti] Título:Forensic characteristics and phylogenetic analysis of Hubei Han population in central China using 17 Y-STR loci.
[So] Source:Forensic Sci Int Genet;29:e4-e8, 2017 07.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Currently, the largest national database within the Y chromosome haplotype reference database (YHRD, https://yhrd.org, release 53) is China, which has approximately 38000 Y chromosomal 17-marker (Yfiler) haplotypes. These haplotype profiles derived from the vast majority of Chinese administrative divisions, but no haplotype data was available for Hubei province, which is located in the Central China region. Herein, 429 unrelated male Chinese Han individuals residing in Hubei province were recruited and genotyped with 17 Y-STR loci. 115 alleles were identified with corresponding allele frequencies spanned from 0.0023 to 07506. The gene diversity (GD) values ranged from 0.3988 at DYS438 to 0.9573 at DYS385a/b. A total of 410 distinct haplotypes were obtained with the overall haplotype diversity (HD) and discrimination capacity (DC) was 0.9995 and 0.9557, respectively. Additionally, genetic relationships along administrative (Han Chinese from different provinces) and ethnic divisions (minority ethnic groups) were analyzed using analysis of molecular variance (AMOVA) tests and visualized by multidimensional scaling plots (MDS). The Han ethnicity including the Hubei Han shows a high genetic homogeneity all across China and significant genetic differences existed between the Hubei Han and some ethnic groups, most prominently for the Kazakhs and the Tibetans.
[Mh] Termos MeSH primário: Cromossomos Humanos Y
Grupos Étnicos/genética
Genética Populacional
Repetições de Microssatélites
[Mh] Termos MeSH secundário: China
Impressões Digitais de DNA
Genótipo
Haplótipos
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  8 / 13755 MEDLINE  
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[PMID]:29099833
[Au] Autor:Andersen MM; Balding DJ
[Ad] Endereço:Department of Mathematical Sciences, Aalborg University, Aalborg, Denmark.
[Ti] Título:How convincing is a matching Y-chromosome profile?
[So] Source:PLoS Genet;13(11):e1007028, 2017 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The introduction of forensic autosomal DNA profiles was controversial, but the problems were successfully addressed, and DNA profiling has gone on to revolutionise forensic science. Y-chromosome profiles are valuable when there is a mixture of male-source and female-source DNA, and interest centres on the identity of the male source(s) of the DNA. The problem of evaluating evidential weight is even more challenging for Y profiles than for autosomal profiles. Numerous approaches have been proposed, but they fail to deal adequately with the fact that men with matching Y-profiles are related in extended patrilineal clans, many of which may not be represented in available databases. The higher mutation rates of modern profiling kits have led to increased discriminatory power but they have also exacerbated the problem of fairly conveying evidential value. Because the relevant population is difficult to define, yet the number of matching relatives is fixed as population size varies, it is typically infeasible to derive population-based match probabilities relevant to a specific crime. We propose a conceptually simple solution, based on a simulation model and software to approximate the distribution of the number of males with a matching Y profile. We show that this distribution is robust to different values for the variance in reproductive success and the population growth rate. We also use importance sampling reweighting to derive the distribution of the number of matching males conditional on a database frequency, finding that this conditioning typically has only a modest impact. We illustrate the use of our approach to quantify the value of Y profile evidence for a court in a way that is both scientifically valid and easily comprehensible by a judge or juror.
[Mh] Termos MeSH primário: Cromossomos Humanos Y/genética
DNA/genética
[Mh] Termos MeSH secundário: Impressões Digitais de DNA/métodos
Genética Forense/métodos
Seres Humanos
Masculino
Probabilidade
Reprodução
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007028


  9 / 13755 MEDLINE  
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[PMID]:28854191
[Au] Autor:Miño CI; de Souza ED; Moralez-Silva E; Valdes TA; Cortiço Corrêa Rodrigues VL; Del Lama SN
[Ad] Endereço:Instituto de Biología Subtropical (IBS), Nodo Iguazú, Universidad Nacional de Misiones (UNaM)-CONICET, Puerto Iguazú, Misiones, Argentina.
[Ti] Título:Use of noninvasive 'bug-eggs' to enable comparative inferences on genetic mating system with and without parental information: A study in a cattle egret colony.
[So] Source:PLoS One;12(8):e0183153, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Colonial waterbirds such as herons, egrets and spoonbills exhibit ecological characteristics that could have promoted the evolution of conspecific brood parasitism and extra-pair copulation. However, an adequate characterization of the genetic mating systems of this avian group has been hindered by the lack of samples of elusive candidate parents which precluded conducting conventional parentage allocation tests. Here, we investigate the genetic mating system of the invasive cattle egret using hematophagous insects contained in fake eggs to collect blood from incubating adults in a wild breeding colony. We tested a protocol with a previously unused Neotropical Triatominae, Panstrongylus megistus, obtained blood samples from males and females in 31 nests built on trees, drew blood from 89 nestlings at those nests, and genotyped all samples at 14 microsatellite loci, including six new species-specific loci. We comparatively addressed the performance of parentage allocation versus kinship classification of nestlings to infer the genetic mating system of cattle egrets. In line with previous behavioral observations, we found evidence in support of a non-monogamous genetic mating system, including extra-pair paternity (EPP) and conspecific brood parasitism (CBP). Parentage allocation tests detected a higher percentage of nests with alternative reproductive tactics (EPP: 61.7%; CBP: 64.5%) than the kinship classification method (EPP: 50.0%; CBP: 43.3%). Overall, these results indicate that rates of alternative reproductive tactics inferred in the absence of parental genetic information could be underestimated and should be interpreted with caution. This study highlights the importance of incorporating samples from candidate parents to adequately determine the genetic mating system of a species. We expand knowledge on the reproductive tactics of colonial waterbirds, contributing novel data on the genetic mating system of the cattle egret, valuable for the design of management strategies for this invasive bird.
[Mh] Termos MeSH primário: Aves/fisiologia
Copulação/fisiologia
Genótipo
Padrões de Herança
Preferência de Acasalamento Animal/fisiologia
Reprodução/genética
[Mh] Termos MeSH secundário: Animais
Brasil
Impressões Digitais de DNA/métodos
Feminino
Técnicas de Genotipagem
Espécies Introduzidas
Masculino
Repetições de Microssatélites
Comportamento de Nidação/fisiologia
Triatominae/química
Triatominae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183153


  10 / 13755 MEDLINE  
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[PMID]:28817590
[Au] Autor:Prendergast ME; Buckley M; Crowther A; Frantz L; Eager H; Lebrasseur O; Hutterer R; Hulme-Beaman A; Van Neer W; Douka K; Veall MA; Quintana Morales EM; Schuenemann VJ; Reiter E; Allen R; Dimopoulos EA; Helm RM; Shipton C; Mwebi O; Denys C; Horton M; Wynne-Jones S; Fleisher J; Radimilahy C; Wright H; Searle JB; Krause J; Larson G; Boivin NL
[Ad] Endereço:Radcliffe Institute for Advanced Study, Harvard University, Cambridge, MA, United States of America.
[Ti] Título:Reconstructing Asian faunal introductions to eastern Africa from multi-proxy biomolecular and archaeological datasets.
[So] Source:PLoS One;12(8):e0182565, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human-mediated biological exchange has had global social and ecological impacts. In sub-Saharan Africa, several domestic and commensal animals were introduced from Asia in the pre-modern period; however, the timing and nature of these introductions remain contentious. One model supports introduction to the eastern African coast after the mid-first millennium CE, while another posits introduction dating back to 3000 BCE. These distinct scenarios have implications for understanding the emergence of long-distance maritime connectivity, and the ecological and economic impacts of introduced species. Resolution of this longstanding debate requires new efforts, given the lack of well-dated fauna from high-precision excavations, and ambiguous osteomorphological identifications. We analysed faunal remains from 22 eastern African sites spanning a wide geographic and chronological range, and applied biomolecular techniques to confirm identifications of two Asian taxa: domestic chicken (Gallus gallus) and black rat (Rattus rattus). Our approach included ancient DNA (aDNA) analysis aided by BLAST-based bioinformatics, Zooarchaeology by Mass Spectrometry (ZooMS) collagen fingerprinting, and direct AMS (accelerator mass spectrometry) radiocarbon dating. Our results support a late, mid-first millennium CE introduction of these species. We discuss the implications of our findings for models of biological exchange, and emphasize the applicability of our approach to tropical areas with poor bone preservation.
[Mh] Termos MeSH primário: Espécies Introduzidas/história
[Mh] Termos MeSH secundário: África
Animais
Animais Domésticos/genética
Arqueologia
Ásia
Galinhas
Colágeno/análise
Colágeno/genética
Impressões Digitais de DNA
História Antiga
Datação Radiométrica
Ratos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182565



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