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[PMID]:29241087
[Au] Autor:Vosough M; Tehrani SM
[Ad] Endereço:Department of Clean Technologies, Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran. Electronic address: vosough@ccerci.ac.ir.
[Ti] Título:Development of a fast HPLC-DAD method for simultaneous quantitation of three immunosuppressant drugs in whole blood samples using intelligent chemometrics resolving of coeluting peaks in the presence of blood interferences.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:69-79, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study describes a fast high performance liquid chromatography-diode array detection analytical methodology for quantification of tacrolimus, everolimus and cyclosporine A in whole blood samples, with minimum sample preparation steps. A short isocratic chromatographic elution was coupled with second-order calibration using multivariate curve resolution to stablish a smart and green methodology. Due to presence of matrix effect, a sample-added calibration strategy was used for quantification purposes. The serious issues related to background drift, chromatographic shifts and co-elution of non-calibrated blood components, were resolved by a proper background correction and multivariate curve resolution-alternating least squares (MCR/ALS) methods The main features of this study were based on the fact that the acquired data matrices were handled intelligently and all features of the concerned target analytes were taken into account. Satisfactory resolution and quantification results in the presence of matrix interferences were achieved and the second-order advantage was fully exploited. The average recoveries in therapeutic concentration ranges were 102±10%, 99±11% and 104±12% for TAC, EVR and CsA, with average relative prediction errors of less than 7%. Considering the advantages of the present strategy, such as increased selectivity, sensitivity and sufficiency of lower limit of quantification through multivariate advantage, simplicity of sample treatment steps, a fast elution pattern and also a low-cost instrumentation compared with LC-MS/MS, the proposed method has the significant merits as an alternative for simultaneous therapeutic monitoring of immunosuppressants.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Imunossupressores/sangue
[Mh] Termos MeSH secundário: Biologia Computacional
Ciclosporina/sangue
Everolimo/sangue
Seres Humanos
Análise dos Mínimos Quadrados
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Tacrolimo/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunosuppressive Agents); 83HN0GTJ6D (Cyclosporine); 9HW64Q8G6G (Everolimus); WM0HAQ4WNM (Tacrolimus)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


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Registro de Ensaios Clínicos
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[PMID]:29443664
[Au] Autor:Mercuri E; Darras BT; Chiriboga CA; Day JW; Campbell C; Connolly AM; Iannaccone ST; Kirschner J; Kuntz NL; Saito K; Shieh PB; Tulinius M; Mazzone ES; Montes J; Bishop KM; Yang Q; Foster R; Gheuens S; Bennett CF; Farwell W; Schneider E; De Vivo DC; Finkel RS; CHERISH Study Group
[Ad] Endereço:From the Department of Pediatric Neurology, Catholic University, Rome (E.M., E.S.M.); the Department of Neurology, Boston Children's Hospital, Boston (B.T.D.), and Biogen, Cambridge (R.F., S.G., W.F.) - both in Massachusetts; the Departments of Neurology (C.A.C., J.M., D.C.D.), Pediatrics (C.A.C., D
[Ti] Título:Nusinersen versus Sham Control in Later-Onset Spinal Muscular Atrophy.
[So] Source:N Engl J Med;378(7):625-635, 2018 02 15.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nusinersen is an antisense oligonucleotide drug that modulates pre-messenger RNA splicing of the survival motor neuron 2 ( SMN2) gene. It has been developed for the treatment of spinal muscular atrophy (SMA). METHODS: We conducted a multicenter, double-blind, sham-controlled, phase 3 trial of nusinersen in 126 children with SMA who had symptom onset after 6 months of age. The children were randomly assigned, in a 2:1 ratio, to undergo intrathecal administration of nusinersen at a dose of 12 mg (nusinersen group) or a sham procedure (control group) on days 1, 29, 85, and 274. The primary end point was the least-squares mean change from baseline in the Hammersmith Functional Motor Scale-Expanded (HFMSE) score at 15 months of treatment; HFMSE scores range from 0 to 66, with higher scores indicating better motor function. Secondary end points included the percentage of children with a clinically meaningful increase from baseline in the HFMSE score (≥3 points), an outcome that indicates improvement in at least two motor skills. RESULTS: In the prespecified interim analysis, there was a least-squares mean increase from baseline to month 15 in the HFMSE score in the nusinersen group (by 4.0 points) and a least-squares mean decrease in the control group (by -1.9 points), with a significant between-group difference favoring nusinersen (least-squares mean difference in change, 5.9 points; 95% confidence interval, 3.7 to 8.1; P<0.001). This result prompted early termination of the trial. Results of the final analysis were consistent with results of the interim analysis. In the final analysis, 57% of the children in the nusinersen group as compared with 26% in the control group had an increase from baseline to month 15 in the HFMSE score of at least 3 points (P<0.001), and the overall incidence of adverse events was similar in the nusinersen group and the control group (93% and 100%, respectively). CONCLUSIONS: Among children with later-onset SMA, those who received nusinersen had significant and clinically meaningful improvement in motor function as compared with those in the control group. (Funded by Biogen and Ionis Pharmaceuticals; CHERISH ClinicalTrials.gov number, NCT02292537 .).
[Mh] Termos MeSH primário: Oligonucleotídeos Antissenso/uso terapêutico
Oligonucleotídeos/uso terapêutico
Atrofias Musculares Espinais da Infância/tratamento farmacológico
[Mh] Termos MeSH secundário: Idade de Início
Criança
Pré-Escolar
Método Duplo-Cego
Feminino
Seres Humanos
Lactente
Injeções Espinhais
Análise dos Mínimos Quadrados
Masculino
Destreza Motora
Oligonucleotídeos/efeitos adversos
Oligonucleotídeos Antissenso/efeitos adversos
Atrofias Musculares Espinais da Infância/fisiopatologia
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE III; COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (Oligonucleotides, Antisense); 0 (nusinersen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180215
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMoa1710504


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[PMID]:29351554
[Au] Autor:Sabry AH; W Hasan WZ; Ab Kadir MZA; Radzi MAM; Shafie S
[Ad] Endereço:Department of Control and Automation, Faculty of Engineering, UPM, Serdang, Malaysia.
[Ti] Título:Field data-based mathematical modeling by Bode equations and vector fitting algorithm for renewable energy applications.
[So] Source:PLoS One;13(1):e0191478, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The power system always has several variations in its profile due to random load changes or environmental effects such as device switching effects when generating further transients. Thus, an accurate mathematical model is important because most system parameters vary with time. Curve modeling of power generation is a significant tool for evaluating system performance, monitoring and forecasting. Several numerical techniques compete to fit the curves of empirical data such as wind, solar, and demand power rates. This paper proposes a new modified methodology presented as a parametric technique to determine the system's modeling equations based on the Bode plot equations and the vector fitting (VF) algorithm by fitting the experimental data points. The modification is derived from the familiar VF algorithm as a robust numerical method. This development increases the application range of the VF algorithm for modeling not only in the frequency domain but also for all power curves. Four case studies are addressed and compared with several common methods. From the minimal RMSE, the results show clear improvements in data fitting over other methods. The most powerful features of this method is the ability to model irregular or randomly shaped data and to be applied to any algorithms that estimating models using frequency-domain data to provide state-space or transfer function for the model.
[Mh] Termos MeSH primário: Algoritmos
Modelos Teóricos
Energia Renovável/estatística & dados numéricos
[Mh] Termos MeSH secundário: Análise dos Mínimos Quadrados
Conceitos Matemáticos
Energia Solar/estatística & dados numéricos
Vento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191478


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[PMID]:29353036
[Au] Autor:Wu Z; Jin L; Zheng W; Zhang C; Zhang L; Chen Y; Guan J; Fei H
[Ad] Endereço:Department of Obstetrics and Gynaecology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, People's Republic of China.
[Ti] Título:NMR-based serum metabolomics study reveals a innovative diagnostic model for missed abortion.
[So] Source:Biochem Biophys Res Commun;496(2):679-685, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A missed abortion (MA) is an in-utero death of the embryo or fetus before the 20th week of gestation with retained products of conception. In order to discover novel biomarkers for MA, a H NMR spectroscopy-based metabolomics approach was applied to detect human MA serum metabolic profiles. Serum samples were obtained from patients with MA (n = 15) and healthy controls (n = 9) for study. The NOESYPR1D spectrum combined with multi-variate pattern recognition analysis was used to cluster the groups and establish a disease-specific metabolites phenotype. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models were capable of distinguishing MA patients from healthy subjects. The results revealed that 24 metabolites altered in MA patients compared with the control population. Metabolomic pathway analysis demonstrated that alanine, aspartate and glutamate metabolism, citrate cycle (TCA cycle), taurine and hypotaurine metabolism were significantly altered in MA. The results indicated that serum NMR-based metabolomic profiling method is sensitive and specific enough to distinguish MA and from healthy controls, this method could be developed as a clinically useful diagnostic tool for MA. The finding from the MA serum metabolic profiling shed a new light on further understanding of MA disease mechanisms.
[Mh] Termos MeSH primário: Aborto Retido/sangue
Aborto Retido/diagnóstico
Espectroscopia de Ressonância Magnética/métodos
Metaboloma
Metabolômica/métodos
[Mh] Termos MeSH secundário: Adulto
Análise Discriminante
Feminino
Seres Humanos
Análise dos Mínimos Quadrados
Gravidez
Análise de Componente Principal
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180122
[St] Status:MEDLINE


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[PMID]:29304173
[Au] Autor:Ren P; Li B; Dong S; Chen L; Zhang Y
[Ad] Endereço:Department of Neurosurgery, Xi'an Jiaotong University School of Medicine, Xi'an, People's Republic of China.
[Ti] Título:The reliability of nonlinear least-squares algorithm for data analysis of neural response activity during sinusoidal rotational stimulation in semicircular canal neurons.
[So] Source:PLoS One;13(1):e0190596, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although many mathematical methods were used to analyze the neural activity under sinusoidal stimulation within linear response range in vestibular system, the reliabilities of these methods are still not reported, especially in nonlinear response range. Here we chose nonlinear least-squares algorithm (NLSA) with sinusoidal model to analyze the neural response of semicircular canal neurons (SCNs) during sinusoidal rotational stimulation (SRS) over a nonlinear response range. Our aim was to acquire a reliable mathematical method for data analysis under SRS in vestibular system. Our data indicated that the reliability of this method in an entire SCNs population was quite satisfactory. However, the reliability was strongly negatively depended on the neural discharge regularity. In addition, stimulation parameters were the vital impact factors influencing the reliability. The frequency had a significant negative effect but the amplitude had a conspicuous positive effect on the reliability. Thus, NLSA with sinusoidal model resulted a reliable mathematical tool for data analysis of neural response activity under SRS in vestibular system and more suitable for those under the stimulation with low frequency but high amplitude, suggesting that this method can be used in nonlinear response range. This method broke out of the restriction of neural activity analysis under nonlinear response range and provided a solid foundation for future study in nonlinear response range in vestibular system.
[Mh] Termos MeSH primário: Algoritmos
Neurônios/fisiologia
Canais Semicirculares/citologia
[Mh] Termos MeSH secundário: Animais
Chinchila
Feminino
Análise dos Mínimos Quadrados
Dinâmica não Linear
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190596


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[PMID]:28946278
[Au] Autor:Caporaso N; Whitworth MB; Fisk ID
[Ad] Endereço:Campden BRI, Chipping Campden, Gloucestershire GL55 6LD, UK; Division of Food Sciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, UK. Electronic address: stxnc7@nottingham.ac.uk.
[Ti] Título:Protein content prediction in single wheat kernels using hyperspectral imaging.
[So] Source:Food Chem;240:32-42, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyperspectral imaging (HSI) combines Near-infrared (NIR) spectroscopy and digital imaging to give information about the chemical properties of objects and their spatial distribution. Protein content is one of the most important quality factors in wheat. It is known to vary widely depending on the cultivar, agronomic and climatic conditions. However, little information is known about single kernel protein variation within batches. The aim of the present work was to measure the distribution of protein content in whole wheat kernels on a single kernel basis, and to apply HSI to predict this distribution. Wheat samples from 2013 and 2014 harvests were sourced from UK millers and wheat breeders, and individual kernels were analysed by HSI and by the Dumas combustion method for total protein content. HSI was applied in the spectral region 980-2500nm in reflectance mode using the push-broom approach. Single kernel spectra were used to develop partial least squares (PLS) regression models for protein prediction of intact single grains. The protein content ranged from 6.2 to 19.8% ("as-is" basis), with significantly higher values for hard wheats. The performance of the calibration model was evaluated using the coefficient of determination (R ) and the root mean square error (RMSE) from 3250 samples used for calibration and 868 used for external validation. The calibration performance for single kernel protein content was R of 0.82 and 0.79, and RMSE of 0.86 and 0.94% for the calibration and validation dataset, enabling quantification of the protein distribution between kernels and even visualisation within the same kernel. The performance of the single kernel measurement was poorer than that typically obtained for bulk samples, but is acceptable for some specific applications. The use of separate calibrations built by separating hard and soft wheat, or on kernels placed on similar orientation did not greatly improve the prediction ability. We simulated the use of the lower cost InGaAs detector (1000-1700nm), and reported that the use of proposed HgCdTe detectors over a restricted spectral range gave a lower prediction error (RMSEC=0.86% vs 1.06%, for HgCdTe and InGaAs, respectively), and increased R value (R =0.82 vs 0.73).
[Mh] Termos MeSH primário: Triticum
[Mh] Termos MeSH secundário: Calibragem
Dureza
Análise dos Mínimos Quadrados
Espectroscopia de Luz Próxima ao Infravermelho
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:25816814
[Au] Autor:Garland B; Wodahl E; Cota L
[Ad] Endereço:Missouri StateUniversity, Springfield, USA BrettGarland@MissouriState.edu.
[Ti] Título:Measuring Public Support for Prisoner Reentry Options.
[So] Source:Int J Offender Ther Comp Criminol;60(12):1406-24, 2016 Sep.
[Is] ISSN:1552-6933
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Few topics have been discussed more extensively or feverishly within correctional academic and professional circles in the past few decades than prisoner reentry. Although program and policy evaluations have been conducted, a lack of public support for prisoner reentry initiatives could undermine the sustainability of prisoner reentry as a large-scale movement. Interestingly, no multivariate, explanatory analyses of the correlates of support for prisoner reentry policies could be found in the literature. This omission is due in part to the absence of clear psychometric measures to assess support. The current study examines the data obtained from a sample of residents in a Midwestern state to determine the dimensionality of support for prisoner reentry interventions using both exploratory factor analysis (EFA) and ordinary least squares (OLS) regression. Specifically, our expectation is that the following three-factor structure will be identified: (a) support for transitional programs aimed at building skills and knowledge to handle the obstacles of the prison-to-community transitional process, (b) support for post-release transitional housing units, and (c) opposition to denying offenders housing opportunities. Our results support a three-factor model. The implications of these findings for future research are discussed.
[Mh] Termos MeSH primário: Integração Comunitária
Prisioneiros
Opinião Pública
[Mh] Termos MeSH secundário: Análise Fatorial
Feminino
Seres Humanos
Análise dos Mínimos Quadrados
Masculino
Meia-Idade
Meio-Oeste dos Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1177/0306624X15578438


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[PMID]:29248446
[Au] Autor:Sanchez Garcia D; Sjödin M; Hellstrandh M; Norinder U; Nikiforova V; Lindberg J; Wincent E; Bergman Å; Cotgreave I; Munic Kos V
[Ad] Endereço:Swetox, Karolinska Institutet, Unit of Toxicology Sciences, Forskargatan 20, SE-151 36 Södertälje, Sweden.
[Ti] Título:Cellular accumulation and lipid binding of perfluorinated alkylated substances (PFASs) - A comparison with lysosomotropic drugs.
[So] Source:Chem Biol Interact;281:1-10, 2018 Feb 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Many chemicals accumulate in organisms through a variety of different mechanisms. Cationic amphiphilic drugs (CADs) accumulate in lysosomes and bind to membranes causing phospholipidosis, whereas many lipophilic chemicals target adipose tissue. Perfluoroalkyl substances (PFASs) are widely used as surfactants, but many of them are highly bioaccumulating and persistent in the environment, making them notorious environmental toxicants. Understanding the mechanisms of their bioaccumulation is, therefore, important for their regulation and substitution with new, less harmful chemicals. We compared the highly bioaccumulative perfluorooctanesulfonic acid PFOS to its three less bioaccumulative alternatives perfluorooctanoic acid (PFOA), perfluorohexanoic acid (PFHxA) and perfluorobutane sulfonic acid (PFBS), in their ability to accumulate and remain in lung epithelial cells (NCI-H292) and adipocytes (3T3-L1K) in vitro. As a reference point we tested a set of cationic amphiphilic drugs (CADs), known to highly accumulate in cells and strongly bind to phospholipids, together with their respective non-CAD controls. Finally, all compounds were examined for their ability to bind to neutral lipids and phospholipids in cell-free systems. Cellular accumulation and retention of the test compounds were highly correlated between the lung epithelial cells and adipocytes. Interestingly, although an anion itself, intensities of PFOS accumulation and retention in cells were comparable to those of CAD compounds, but PFOS failed to induce phospholipidosis or alter lysosomal volume. Compared to other lipophilicity measures, phospholipophilicity shows the highest correlation (Rˆ2 = 0.75) to cellular accumulation data in both cell types and best distinguishes between high and low accumulating compounds. This indicates that binding to phospholipids may be the most important component in driving high cellular accumulation in lung epithelial cells, as well as in adipocytes, and for both CADs and bioaccumulating PFASs. Obtained continuous PLS models based on compound's affinity for phospholipids and neutral lipids can be used as good prediction models of cellular accumulation and retention of PFASs and CADs.
[Mh] Termos MeSH primário: Ácidos Alcanossulfônicos/metabolismo
Fluorcarbonetos/metabolismo
Lisossomos/metabolismo
Preparações Farmacêuticas/metabolismo
Fosfolipídeos/metabolismo
[Mh] Termos MeSH secundário: Adipócitos/citologia
Adipócitos/metabolismo
Ácidos Alcanossulfônicos/química
Animais
Azitromicina/química
Azitromicina/metabolismo
Caproatos/química
Caproatos/metabolismo
Caprilatos/química
Caprilatos/metabolismo
Cátions/química
Linhagem Celular
Sobrevivência Celular
Células Epiteliais/citologia
Células Epiteliais/metabolismo
Fluorcarbonetos/química
Seres Humanos
Análise dos Mínimos Quadrados
Modelos Lineares
Lipídeos/química
Camundongos
Preparações Farmacêuticas/química
Fosfolipídeos/química
Ácidos Sulfônicos/química
Ácidos Sulfônicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanesulfonic Acids); 0 (Caproates); 0 (Caprylates); 0 (Cations); 0 (Fluorocarbons); 0 (Lipids); 0 (Pharmaceutical Preparations); 0 (Phospholipids); 0 (Sulfonic Acids); 0 (perfluorobutanesulfonic acid); 83905-01-5 (Azithromycin); 947VD76D3L (perfluorooctanoic acid); 9H2MAI21CL (perfluorooctane sulfonic acid); ZP34Q2220R (perfluorohexanoic acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


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[PMID]:29232626
[Au] Autor:Wen C; Wang D; Li X; Huang T; Huang C; Hu K
[Ad] Endereço:State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Targeted isolation and identification of bioactive compounds lowering cholesterol in the crude extracts of crabapples using UPLC-DAD-MS-SPE/NMR based on pharmacology-guided PLS-DA.
[So] Source:J Pharm Biomed Anal;150:144-151, 2018 Feb 20.
[Is] ISSN:1873-264X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The anti-hyperlipidemic effects of crude crabapple extracts derived from Malus 'Red jade', Malus hupehensis (Pamp.) Rehd. and Malus prunifolia (Willd.) Borkh. were evaluated on high-fat diet induced obese (HF DIO) mice. The results revealed that some of these extracts could lower serum cholesterol levels in HF DIO mice. The same extracts were also parallelly analyzed by LC-MS in both positive and negative ionization modes. Based on the pharmacological results, 22 LC-MS variables were identified to be correlated with the anti-hyperlipidemic effects using partial least square discriminant analysis (PLS-DA) and independent samples t-test. Further, under the guidance of the bioactivity-correlated LC-MS signals, 10 compounds were targetedly isolated and enriched using UPLC-DAD-MS-SPE and identified/elucidated by NMR together with MS/MS as citric acid(1), p-coumaric acid(2), hyperoside(3), myricetin(4), naringenin(5), quercetin(6), kaempferol(7), gentiopicroside(8), ursolic acid(9) and 8-epiloganic acid(10). Among these 10 compounds, 6 compounds, hyperoside(3), myricetin(4), naringenin(5), quercetin(6), kaempferol(7) and ursolic acid(9), were individually studied and reported to indeed have effects on lowering the serum lipid levels. These results demonstrated the efficiency of this strategy for drug discovery. In contrast to traditional routes to discover bioactive compounds in the plant extracts, targeted isolation and identification of bioactive compounds in the crude plant extracts using UPLC-DAD-MS-SPE/NMR based on pharmacology-guided PLS-DA of LC-MS data brings forward a new efficient dereplicated approach to natural products research for drug discovery.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Hipolipemiantes/isolamento & purificação
Malus/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Animais
Colesterol/sangue
Cromatografia Líquida/métodos
Análise Discriminante
Feminino
Hipolipemiantes/análise
Hipolipemiantes/farmacologia
Análise dos Mínimos Quadrados
Espectroscopia de Ressonância Magnética
Espectrometria de Massas/métodos
Camundongos
Camundongos Endogâmicos C57BL
Obesidade/tratamento farmacológico
Extratos Vegetais/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypolipidemic Agents); 0 (Plant Extracts); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:29261760
[Au] Autor:Kabir A; Rahman MJ; Shamim AA; Klemm RDW; Labrique AB; Rashid M; Christian P; West KP
[Ad] Endereço:Department of Statistics, University of Rajshahi, Rajshahi, Bangladesh.
[Ti] Título:Identifying maternal and infant factors associated with newborn size in rural Bangladesh by partial least squares (PLS) regression analysis.
[So] Source:PLoS One;12(12):e0189677, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Birth weight, length and circumferences of the head, chest and arm are key measures of newborn size and health in developing countries. We assessed maternal socio-demographic factors associated with multiple measures of newborn size in a large rural population in Bangladesh using partial least squares (PLS) regression method. PLS regression, combining features from principal component analysis and multiple linear regression, is a multivariate technique with an ability to handle multicollinearity while simultaneously handling multiple dependent variables. We analyzed maternal and infant data from singletons (n = 14,506) born during a double-masked, cluster-randomized, placebo-controlled maternal vitamin A or ß-carotene supplementation trial in rural northwest Bangladesh. PLS regression results identified numerous maternal factors (parity, age, early pregnancy MUAC, living standard index, years of education, number of antenatal care visits, preterm delivery and infant sex) significantly (p<0.001) associated with newborn size. Among them, preterm delivery had the largest negative influence on newborn size (Standardized ß = -0.29 - -0.19; p<0.001). Scatter plots of the scores of first two PLS components also revealed an interaction between newborn sex and preterm delivery on birth size. PLS regression was found to be more parsimonious than both ordinary least squares regression and principal component regression. It also provided more stable estimates than the ordinary least squares regression and provided the effect measure of the covariates with greater accuracy as it accounts for the correlation among the covariates and outcomes. Therefore, PLS regression is recommended when either there are multiple outcome measurements in the same study, or the covariates are correlated, or both situations exist in a dataset.
[Mh] Termos MeSH primário: Mães
População Rural
[Mh] Termos MeSH secundário: Adulto
Bangladesh
Seres Humanos
Recém-Nascido
Análise dos Mínimos Quadrados
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189677



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