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[PMID]:29381969
[Au] Autor:Rad A; Sørbye SW; Dreyer G; Hovland S; Falang BM; Louw M; Skjeldestad FE
[Ad] Endereço:Department of Community Medicine, Faculty of Health Sciences, UiT The Arctic University of Norway.
[Ti] Título:HPV types in cervical cancer tissue in South Africa: A head-to-head comparison by mRNA and DNA tests.
[So] Source:Medicine (Baltimore);96(47):e8752, 2017 Nov.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accurate identification of human papillomavirus (HPV)-types in cervical cancer tissue may be important for tailoring tests for primary screening and types to be included in a vaccine. The aim of this study was to compare test-performance of a 45-type HPV deoxyribonucleic acid (DNA)-test with a 9-type HPV messenger ribonucleic acid (mRNA)-test in cervical cancer tissues.In a case-series design 188 women with diagnosed cervical cancer during the period January 2008 to July 1, 2011 at the Gynaecological Oncology Unit, University of Pretoria, South Africa were recruited to the study. After cases with negative internal controls for DNA/mRNA detection (n = 18) and unconfirmed histology (n = 3) of cervical cancer were excluded, 167 women remained eligible for analysis. We compared 45 DNA-types detected through general primer (GP)5/6 polymerase chain reaction (PCR) and reverse line blot (RLB) genotyping with a modified version of the mRNA test PreTect HPV-Proofer detecting 9 genotypes (16, 18, 31, 33, 35, 45, 51, 52, 58).Histological types were 92.2% squamous cell carcinoma, 4.8% adenocarcinoma, and 3.0% adenosquamous carcinoma. Overall, HPV was detected in 95.2% (159/167) of specimens. The DNA- and mRNA tests each rendered 153/167 (91.6%) HPV positive results. When restricting the analysis to the 9 high-risk HPV-types included in the mRNA test, 91.6% (153/167) and 88.0% (147/167) were positive by the mRNA- and DNA-tests (P = .28), respectively. After hierarchical categorization of 9 comparable types, we found concordance in 66 of 67 specimens for HPV16, 25 of 27 specimens for HPV18, 19 of 21 specimens for HPV45, and only in 33 of 45 for HPV31, 33, 35, 51, 52, 58. The positivity rate for the HPV types 16, 18, and 45 and the positivity rate for HPV 16, 18, 45, 33 and 35 by both tests was 66% to 68% and 80% to 83%, respectively.Overall and when considering established high-risk types, the mRNA test has at least as high detection rate as the DNA test. The mRNA test can be an appropriate research tool to describe causative HPV-types in cervical cancer tissue for health care planning purposes.
[Mh] Termos MeSH primário: Técnicas Genéticas
Papillomaviridae/genética
Infecções por Papillomavirus/virologia
Neoplasias do Colo do Útero/virologia
[Mh] Termos MeSH secundário: Adenocarcinoma/virologia
Carcinoma Adenoescamoso/virologia
Carcinoma de Células Escamosas/virologia
DNA Viral
Feminino
Seres Humanos
Reação em Cadeia da Polimerase
RNA Mensageiro
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (RNA, Messenger)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008752


  2 / 11161 MEDLINE  
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[PMID]:29304036
[Au] Autor:Oren E; Klingeman W; Gazis R; Moulton J; Lambdin P; Coggeshall M; Hulcr J; Seybold SJ; Hadziabdic D
[Ad] Endereço:Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, United States of America.
[Ti] Título:A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease.
[So] Source:PLoS One;13(1):e0185087, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thousand Cankers Disease (TCD) of Juglans and Pterocarya (Juglandaceae) involves a fungal pathogen, Geosmithia morbida, and a primary insect vector, Pityophthorus juglandis. TCD was described originally from dying Juglans nigra trees in the western United States (USA), but it was reported subsequently from the eastern USA and northern Italy. The disease is often difficult to diagnose due to the absence of symptoms or signs on the bark surface of the host. Furthermore, disease symptoms can be confused with those caused by other biotic and abiotic agents. Thus, there is a critical need for a method for rapid detection of the pathogen and vector of TCD. Using species-specific microsatellite DNA markers, we developed a molecular protocol for the detection of G. morbida and P. juglandis. To demonstrate the utility of the method for delineating TCD quarantine zones, we tested whether geographical occurrence of symptoms and signs of TCD was correlated with molecular evidence for the presence of the cryptic TCD organisms. A total of 1600 drill cores were taken from branch sections collected from three regions (n = 40 trees for each location): California-J. hindsii (heavy disease incidence); Tennessee-J. nigra (mild disease incidence); and outside the known TCD zone (Missouri-J. nigra, no record of the disease). California samples had the highest incidence of the TCD organisms (85%, 34/40). Tennessee had intermediate incidence (42.5%, 17/40), whereas neither organism was detected in samples from Missouri. The low cost molecular protocol developed here has a high degree of sensitivity and specificity, and it significantly reduces sample-processing time, making the protocol a powerful tool for rapid detection of TCD.
[Mh] Termos MeSH primário: Hypocreales/genética
Hypocreales/patogenicidade
Insetos Vetores/genética
Insetos Vetores/microbiologia
Juglans/microbiologia
Doenças das Plantas/microbiologia
Gorgulhos/genética
Gorgulhos/microbiologia
[Mh] Termos MeSH secundário: Animais
California
DNA Fúngico/genética
Técnicas Genéticas
Hypocreales/isolamento & purificação
Repetições de Microssatélites
Missouri
Especificidade da Espécie
Tennessee
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185087


  3 / 11161 MEDLINE  
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[PMID]:29186505
[Au] Autor:Oudelaar AM; Davies JOJ; Downes DJ; Higgs DR; Hughes JR
[Ad] Endereço:Medical Research Council (MRC) Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.
[Ti] Título:Robust detection of chromosomal interactions from small numbers of cells using low-input Capture-C.
[So] Source:Nucleic Acids Res;45(22):e184, 2017 Dec 15.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin architecture in rare cell populations. We present a new low-input Capture-C approach that can generate high-quality 3C interaction profiles from 10 000-20 000 cells, depending on the resolution used for analysis. We also present a PCR-free, sequencing-free 3C technique based on NanoString technology called C-String. By comparing C-String and Capture-C interaction profiles we show that the latter are not skewed by PCR amplification. Furthermore, we demonstrate that chromatin interactions detected by Capture-C do not depend on the degree of cross-linking by performing experiments with varying formaldehyde concentrations.
[Mh] Termos MeSH primário: Cromatina/metabolismo
Cromossomos/metabolismo
Técnicas Genéticas
Nanotecnologia/métodos
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Células Cultivadas
Cromatina/química
Cromatina/genética
Cromossomos/química
Cromossomos/genética
Feminino
Formaldeído/química
Regulação da Expressão Gênica
Camundongos Endogâmicos C57BL
Conformação Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx1194


  4 / 11161 MEDLINE  
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[PMID]:29223150
[Au] Autor:Filippova JA; Semenov DV; Juravlev ES; Komissarov AB; Richter VA; Stepanov GA
[Ad] Endereço:Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. stepanovga@niboch.nsc.ru.
[Ti] Título:Modern Approaches for Identification of Modified Nucleotides in RNA.
[So] Source:Biochemistry (Mosc);82(11):1217-1233, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies. The development of novel methods for detection of chemical modifications of RNA nucleotides in the cells of living organisms and accumulation of knowledge on the contribution of modified monomers to metabolism and functioning of individual RNA species establish the basis for creation of novel diagnostic and therapeutic approaches. This review includes a short description of routine methods for determination of modified nucleotides in RNA and considers in detail modern approaches that enable not only detection but also quantitative assessment of the modification level of various nucleotides in individual RNA species.
[Mh] Termos MeSH primário: Nucleotídeos/química
Processamento Pós-Transcricional do RNA
RNA/genética
[Mh] Termos MeSH secundário: Animais
Técnicas de Química Analítica/instrumentação
Técnicas de Química Analítica/métodos
Cromatografia Líquida de Alta Pressão
Técnicas Genéticas
Seres Humanos
Espectrometria de Massas
Métodos
Nucleotídeos/análise
Transcrição Reversa
Ribonucleases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nucleotides); 63231-63-0 (RNA); EC 3.1.- (Ribonucleases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110013


  5 / 11161 MEDLINE  
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[PMID]:28985360
[Au] Autor:Gorman KT; Roby LC; Giuffre A; Huang R; Kay BK
[Ad] Endereço:Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60622, USA.
[Ti] Título:Tandem phage-display for the identification of non-overlapping binding pairs of recombinant affinity reagents.
[So] Source:Nucleic Acids Res;45(18):e158, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to the target. To bridge this gap, we invented Megaprimer Shuffling for Tandem Affinity Reagents (MegaSTAR) to identify non-competitive binding pairs of recombinant affinity reagents through phage-display. The key innovation in MegaSTAR is the construction of a tandem library, in which two reagents are randomly-displayed on the phage surface. This is accomplished by using a pool of 300-nucleotide long 'megaprimers', which code for previously-selected reagents, to prime second strand synthesis of a single-stranded DNA template and generate millions of pair-wise combinations. The tandem library is then affinity selected to isolate pairs that both reagents contribute to binding the target. As a proof-of-concept, we used MegaSTAR to identify pairs of fibronectin type III monobodies for three human proteins. For each target, we could identify between five and fifteen unique pairs and successfully used a single pair in a sandwich assay. MegaSTAR is a versatile tool for generating sandwich ELISA-grade and bispecific reagents.
[Mh] Termos MeSH primário: Marcadores de Afinidade/metabolismo
Técnicas de Visualização da Superfície Celular/métodos
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/metabolismo
[Mh] Termos MeSH secundário: Ensaio de Imunoadsorção Enzimática
Técnicas Genéticas
Ensaios de Triagem em Larga Escala/métodos
Seres Humanos
Biblioteca de Peptídeos
Polimerização
Ligação Proteica
Proteínas Recombinantes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Affinity Labels); 0 (Peptide Library); 0 (Recombinant Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx688


  6 / 11161 MEDLINE  
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[PMID]:28870990
[Au] Autor:Vergara MN; Flores-Bellver M; Aparicio-Domingo S; McNally M; Wahlin KJ; Saxena MT; Mumm JS; Canto-Soler MV
[Ad] Endereço:The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA natalia.vergara@ucdenver.edu valeria.canto-soler@ucdenver.edu.
[Ti] Título:Three-dimensional automated reporter quantification (3D-ARQ) technology enables quantitative screening in retinal organoids.
[So] Source:Development;144(20):3698-3705, 2017 10 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The advent of stem cell-derived retinal organoids has brought forth unprecedented opportunities for developmental and physiological studies, while presenting new therapeutic promise for retinal degenerative diseases. From a translational perspective, organoid systems provide exciting new prospects for drug discovery, offering the possibility to perform compound screening in a three-dimensional (3D) human tissue context that resembles the native histoarchitecture and to some extent recapitulates cellular interactions. However, inherent variability issues and a general lack of robust quantitative technologies for analyzing organoids on a large scale pose severe limitations for their use in translational applications. To address this need, we have developed a screening platform that enables accurate quantification of fluorescent reporters in complex human iPSC-derived retinal organoids. This platform incorporates a fluorescence microplate reader that allows -dimensional detection and fine-tuned wavelength selection. We have established optimal parameters for fluorescent reporter signal detection, devised methods to compensate for organoid size variability, evaluated performance and sensitivity parameters, and validated this technology for functional applications.
[Mh] Termos MeSH primário: Técnicas Genéticas
Células-Tronco Pluripotentes Induzidas/citologia
Organoides/fisiologia
Retina/fisiologia
[Mh] Termos MeSH secundário: Técnicas de Cultura de Células
Diferenciação Celular
Linhagem Celular
Corantes Fluorescentes
Genes Reporter
Seres Humanos
Microscopia de Fluorescência
Estresse Oxidativo
Transplante de Células-Tronco
Transgenes
Pesquisa Médica Translacional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1242/dev.146290


  7 / 11161 MEDLINE  
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[PMID]:28841410
[Au] Autor:Liu X; Zhang Y; Chen Y; Li M; Zhou F; Li K; Cao H; Ni M; Liu Y; Gu Z; Dickerson KE; Xie S; Hon GC; Xuan Z; Zhang MQ; Shao Z; Xu J
[Ad] Endereço:Children's Medical Center Research Institute, Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
[Ti] Título:In Situ Capture of Chromatin Interactions by Biotinylated dCas9.
[So] Source:Cell;170(5):1028-1043.e19, 2017 Aug 24.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chromatin-regulating protein complexes and long-range DNA interactions. Using an in vivo biotinylated nuclease-deficient Cas9 protein and sequence-specific guide RNAs, we show high-resolution and selective isolation of chromatin interactions at a single-copy genomic locus. Purification of human telomeres using CAPTURE identifies known and new telomeric factors. In situ capture of individual constituents of the enhancer cluster controlling human ß-globin genes establishes evidence for composition-based hierarchical organization. Furthermore, unbiased analysis of chromatin interactions at disease-associated cis-elements and developmentally regulated super-enhancers reveals spatial features that causally control gene transcription. Thus, comprehensive and unbiased analysis of locus-specific regulatory composition provides mechanistic insight into genome structure and function in development and disease.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Endonucleases/metabolismo
Técnicas Genéticas
Elementos Reguladores de Transcrição
[Mh] Termos MeSH secundário: Animais
Biotinilação
Células Cultivadas
Células-Tronco Embrionárias/metabolismo
Endonucleases/genética
Elementos Facilitadores Genéticos
Seres Humanos
Células K562
Camundongos
RNA Guia/metabolismo
Telômero/metabolismo
Globinas beta/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Guide); 0 (beta-Globins); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  8 / 11161 MEDLINE  
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[PMID]:28817122
[Au] Autor:Kim JW; Seo D; Lee JU; Southard KM; Lim Y; Kim D; Gartner ZJ; Jun YW; Cheon J
[Ad] Endereço:Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.
[Ti] Título:Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles.
[So] Source:Nat Protoc;12(9):1871-1889, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spatiotemporal interrogation of signal transduction at the single-cell level is necessary to answer a host of important biological questions. This protocol describes a nanotechnology-based single-cell and single-molecule perturbation tool, termed mechanogenetics, that enables precise spatial and mechanical control over genetically encoded cell-surface receptors in live cells. The key components of this tool are a magnetoplasmonic nanoparticle (MPN) actuator that delivers defined spatial and mechanical cues to receptors through target-specific one-to-one engagement and a micromagnetic tweezers (µMT) that remotely controls the magnitude of force exerted on a single MPN. In our approach, a SNAP-tagged cell-surface receptor of interest is conjugated with a single-stranded DNA oligonucleotide, which hybridizes to its complementary oligonucleotide on the MPN. This protocol consists of four major stages: (i) chemical synthesis of MPNs, (ii) conjugation with DNA and purification of monovalent MPNs, (iii) modular targeting of MPNs to cell-surface receptors, and (iv) control of spatial and mechanical properties of targeted mechanosensitive receptors in live cells by adjusting the µMT-to-MPN distance. Using benzylguanine (BG)-functionalized MPNs and model cell lines expressing either SNAP-tagged Notch or vascular endothelial cadherin (VE-cadherin), we provide stepwise instructions for mechanogenetic control of receptor clustering and for mechanical receptor activation. The ability of this method to differentially control spatial and mechanical inputs to targeted receptors makes it particularly useful for interrogating the differential contributions of each individual cue to cell signaling. The entire procedure takes up to 1 week.
[Mh] Termos MeSH primário: DNA/metabolismo
Imãs/química
Nanopartículas/metabolismo
Análise de Célula Única/métodos
[Mh] Termos MeSH secundário: Fenômenos Biomecânicos/fisiologia
Linhagem Celular Tumoral
DNA/química
Técnicas Genéticas
Seres Humanos
Fenômenos Mecânicos
Nanopartículas/química
Nanotecnologia/métodos
Receptores de Superfície Celular/química
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Cell Surface); 9007-49-2 (DNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.071


  9 / 11161 MEDLINE  
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[PMID]:28796234
[Au] Autor:Martell JD; Deerinck TJ; Lam SS; Ellisman MH; Ting AY
[Ad] Endereço:Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
[Ti] Título:Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells.
[So] Source:Nat Protoc;12(9):1792-1816, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Electron microscopy (EM) is the premiere technique for high-resolution imaging of cellular ultrastructure. Unambiguous identification of specific proteins or cellular compartments in electron micrographs, however, remains challenging because of difficulties in delivering electron-dense contrast agents to specific subcellular targets within intact cells. We recently reported enhanced ascorbate peroxidase 2 (APEX2) as a broadly applicable genetic tag that generates EM contrast on a specific protein or subcellular compartment of interest. This protocol provides guidelines for designing and validating APEX2 fusion constructs, along with detailed instructions for cell culture, transfection, fixation, heavy-metal staining, embedding in resin, and EM imaging. Although this protocol focuses on EM in cultured mammalian cells, APEX2 is applicable to many cell types and contexts, including intact tissues and organisms, and is useful for numerous applications beyond EM, including live-cell proteomic mapping. This protocol, which describes procedures for sample preparation from cell monolayers and cell pellets, can be completed in 10 d, including time for APEX2 fusion construct validation, cell growth, and solidification of embedding resins. Notably, the only additional steps required relative to a standard EM sample preparation are cell transfection and a 2- to 45-min staining period with 3,3-diaminobenzidine (DAB) and hydrogen peroxide (H O ).
[Mh] Termos MeSH primário: Ascorbato Peroxidases/genética
Técnicas Citológicas/métodos
Técnicas Genéticas
Microscopia Eletrônica/métodos
Imagem Molecular/métodos
[Mh] Termos MeSH secundário: Animais
Células COS
Células Cultivadas
Estruturas Celulares/ultraestrutura
Cercopithecus aethiops
Células HEK293
Hipocampo/citologia
Seres Humanos
Ratos
Proteínas Recombinantes de Fusão/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Fusion Proteins); EC 1.11.1.11 (Ascorbate Peroxidases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.065


  10 / 11161 MEDLINE  
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[PMID]:28790452
[Au] Autor:Claesson MJ; Clooney AG; O'Toole PW
[Ad] Endereço:School of Microbiology, University College Cork, Western Road, T12 Y337 Cork, Ireland.
[Ti] Título:A clinician's guide to microbiome analysis.
[So] Source:Nat Rev Gastroenterol Hepatol;14(10):585-595, 2017 Oct.
[Is] ISSN:1759-5053
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microbiome analysis involves determining the composition and function of a community of microorganisms in a particular location. For the gastroenterologist, this technology opens up a rapidly evolving set of challenges and opportunities for generating novel insights into the health of patients on the basis of microbiota characterizations from intestinal, hepatic or extraintestinal samples. Alterations in gut microbiota composition correlate with intestinal and extraintestinal disease and, although only a few mechanisms are known, the microbiota are still an attractive target for developing biomarkers for disease detection and management as well as potential therapeutic applications. In this Review, we summarize the major decision points confronting new entrants to the field or for those designing new projects in microbiome research. We provide recommendations based on current technology options and our experience of sequencing platform choices. We also offer perspectives on future applications of microbiome research, which we hope convey the promise of this technology for clinical applications.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Microbioma Gastrointestinal
Metagenômica/métodos
[Mh] Termos MeSH secundário: Técnicas Genéticas
Seres Humanos
Projetos de Pesquisa
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1038/nrgastro.2017.97



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