Base de dados : MEDLINE
Pesquisa : E05.393.240 [Categoria DeCS]
Referências encontradas : 5226 [refinar]
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  1 / 5226 MEDLINE  
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[PMID]:28463020
[Au] Autor:Saini M; Selokar NL; Agrawal H; Singla SK; Chauhan MS; Manik RS; Palta P
[Ad] Endereço:Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute , Karnal, India .
[Ti] Título:Treatment of Donor Cells and Reconstructed Embryos with a Combination of Trichostatin-A and 5-aza-2'-Deoxycytidine Improves the Developmental Competence and Quality of Buffalo Embryos Produced by Handmade Cloning and Alters Their Epigenetic Status and Gene Expression.
[So] Source:Cell Reprogram;19(3):208-215, 2017 Jun.
[Is] ISSN:2152-4998
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for altering the epigenetic status of cloned embryos. We optimized the doses of these epigenetic modifiers for production of buffalo embryos by handmade cloning and examined whether combined treatment with these epigenetic modifiers offered any advantage over treatment with the individual epigenetic modifier. Irrespective of whether donor cells or reconstructed embryos or both were treated with 50 nM TSA +7.5 nM 5-aza-dC, (1) the blastocyst rate was significantly higher (71.6 ± 3.5, 68.3 ± 2.6, and 71.8 ± 2.4, respectively, vs. 43.1 ± 3.4 for controls, p < 0.05); (2) the apoptotic index was lower (5.4 ± 1.1, 9.5 ± 1.0, and 7.4 ± 1.3, respectively, vs. 19.5 ± 2.1 for controls, p < 0.05) and was similar to that of in vitro fertilization blastocysts (6.0 ± 0.8); (3) the global level of H3K18ac was higher (p < 0.01) and that of H3K27me3 lower (p < 0.05) than in controls and was similar among all treatment groups; and (4) the expression level of epigenetic-(HDAC1, DNMT1, and DNMT3a), pluripotency-(OCT4 and NANOG), and development-related (FGF4) genes, but not that of SOX2 and CDX2, was similar among all treatment groups. These results demonstrate that similar levels of beneficial effects can be obtained following treatment of either donor cells or reconstructed embryos or both with the combination of TSA +5-aza-dC. Therefore, there is no advantage in treating both donor cells and reconstructed embryos when the combination of TSA and 5-aza-dC is used.
[Mh] Termos MeSH primário: Azacitidina/análogos & derivados
Búfalos
Clonagem de Organismos/métodos
Embrião de Mamíferos/metabolismo
Epigênese Genética/efeitos dos fármacos
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Ácidos Hidroxâmicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Azacitidina/farmacologia
Búfalos/embriologia
Búfalos/genética
Embrião de Mamíferos/citologia
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydroxamic Acids); 3X2S926L3Z (trichostatin A); 776B62CQ27 (decitabine); M801H13NRU (Azacitidine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1089/cell.2016.0061


  2 / 5226 MEDLINE  
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[PMID]:28468955
[Au] Autor:Kaminuma O; Katayama K; Inoue K; Saeki M; Nishimura T; Kitamura N; Shimo Y; Tofukuji S; Ishida S; Ogonuki N; Kamimura S; Oikawa M; Katoh S; Mori A; Shichijo M; Hiroi T; Ogura A
[Ad] Endereço:Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan osamuk@yamanashi.ac.jp ogura@rtc.riken.go.jp.
[Ti] Título:Hyper-reactive cloned mice generated by direct nuclear transfer of antigen-specific CD4 T cells.
[So] Source:EMBO Rep;18(6):885-893, 2017 Jun.
[Is] ISSN:1469-3178
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:T-cell receptor (TCR)-transgenic mice have been employed for evaluating antigen-response mechanisms, but their non-endogenous TCR might induce immune response differently than the physiologically expressed TCR Nuclear transfer cloning produces animals that retain the donor genotype in all tissues including germline and immune systems. Taking advantage of this feature, we generated cloned mice that carry endogenously rearranged TCR genes from antigen-specific CD4 T cells. We show that T cells of the cloned mice display distinct developmental pattern and antigen reactivity because of their endogenously pre-rearranged TCRα (rTα) and TCRß (rTß) alleles. These alleles were transmitted to the offspring, allowing us to establish a set of mouse lines that show chronic-type allergic phenotypes, that is, bronchial and nasal inflammation, upon local administrations of the corresponding antigens. Intriguingly, the existence of either rTα or rTß is sufficient to induce hypersensitivity. These cloned mice expressing intrinsic promoter-regulated antigen-specific TCR are a unique animal model with allergic predisposition for investigating CD4 T-cell-mediated pathogenesis and cellular commitment in immune diseases.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Hipersensibilidade/imunologia
Técnicas de Transferência Nuclear
Receptores de Antígenos de Linfócitos T/genética
[Mh] Termos MeSH secundário: Alelos
Animais
Antígenos/administração & dosagem
Antígenos/imunologia
Clonagem de Organismos
Modelos Animais de Doenças
Camundongos
Camundongos Transgênicos
Receptores de Antígenos de Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Receptors, Antigen, T-Cell)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.15252/embr.201643321


  3 / 5226 MEDLINE  
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[PMID]:29326255
[Au] Autor:Shultz D
[Ti] Título:Creating a modern monster.
[So] Source:Science;359(6372):151, 2018 Jan 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Órgãos Artificiais
Biônica
Clonagem de Organismos
Técnicas de Cultura de Órgãos
Transplantes
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1126/science.359.6372.151


  4 / 5226 MEDLINE  
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[PMID]:28980654
[Au] Autor:Cyranoski D
[Ti] Título:Chinese scientists fix genetic disorder in cloned human embryos.
[So] Source:Nature;550(7674):15-16, 2017 10 02.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Clonagem de Organismos
Técnicas de Transferência Nuclear
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1038/nature.2017.22694


  5 / 5226 MEDLINE  
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[PMID]:28800640
[Au] Autor:Macedo-Silva T; Araujo RBD; Meissner KA; Fotoran WL; Medeiros MM; de Azevedo MF; Wunderlich G
[Ad] Endereço:Department of Parasitology, Institute for Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 1374, São Paulo, Brazil.
[Ti] Título:Knockdown of the Plasmodium falciparum SURFIN4.1 antigen leads to an increase of its cognate transcript.
[So] Source:PLoS One;12(8):e0183129, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genome of the malaria parasite Plasmodium falciparum contains the surf gene family which encodes large transmembrane proteins of unknown function. While some surf alleles appear to be expressed in sexual stages, others occur in asexual blood stage forms and may be associated to virulence-associated processes and undergo transcriptional switching. We accessed the transcription of surf genes along multiple invasions by real time PCR. Based on the observation of persistent expression of gene surf4.1, we created a parasite line which expresses a conditionally destabilized SURFIN4.1 protein. Upon destabilization of the protein, no interference of parasite growth or morphological changes were detected. However, we observed a strong increase in the transcript quantities of surf4.1 and sometimes of other surf genes in knocked-down parasites. While this effect was reversible when SURFIN4.1 was stabilized again after a few days of destabilization, longer destabilization periods resulted in a transcriptional switch away from surf4.1. When we tested if a longer transcript half-life was responsible for increased transcript detection in SURFIN4.1 knocked-down parasites, no alteration was found compared to control parasite lines. This suggests a specific feedback of the expressed SURFIN protein to its transcript pointing to a novel type of regulation, inedited in Plasmodium.
[Mh] Termos MeSH primário: Antígenos de Protozoários/genética
Retroalimentação Fisiológica
Estágios do Ciclo de Vida/genética
Plasmodium falciparum/genética
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Alelos
Antígenos de Protozoários/metabolismo
Clonagem de Organismos
Eritrócitos/parasitologia
Regulação da Expressão Gênica
Técnicas de Silenciamento de Genes
Meia-Vida
Seres Humanos
Morfolinas/farmacologia
Plasmodium falciparum/crescimento & desenvolvimento
Plasmodium falciparum/metabolismo
Isoformas de Proteínas/antagonistas & inibidores
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Estabilidade de RNA
RNA Mensageiro/agonistas
RNA Mensageiro/metabolismo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Morpholines); 0 (Protein Isoforms); 0 (RNA, Messenger); 0 (Shield-1 compound)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183129


  6 / 5226 MEDLINE  
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[PMID]:28545049
[Au] Autor:Wani NA; Vettical BS; Hong SB
[Ad] Endereço:Reproductive Biotechnology Center, Dubai, UAE.
[Ti] Título:First cloned Bactrian camel (Camelus bactrianus) calf produced by interspecies somatic cell nuclear transfer: A step towards preserving the critically endangered wild Bactrian camels.
[So] Source:PLoS One;12(5):e0177800, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Studies were conducted to explore the possibility of employing dromedary camel (Camelus dromedarius) oocytes as recipient cytoplasts for the development of interspecies somatic cell nuclear transfer (iSCNT) embryos using skin fibroblast cells of an adult Bactrian camel (Camelus bactrianus) and Llama (Llama glama) as donor nuclei. Also, the embryos reconstructed with Bactrian cells were transferred into the uterus of synchronized dromedary camel recipients to explore the possibility of using them as surrogate mothers. Serum-starved skin fibroblast cells were injected into the perivitelline space of enucleated mature oocytes, collected from super-stimulated dromedary camels, and fused using an Eppendorf electroporator. After activation with 5µM ionomycin and 6-dimethylaminopurine, they were cultured at 38.5°C in an atmosphere of 5% CO2, 5% O2, and 90% N2 in air. In experiment 1, Day 7 blastocysts were stained with Hoechst to count their cell numbers, while in experiment 2, they were transferred to synchronized dromedary recipients. A lower number (P < 0.05) of blastocysts were obtained from reconstructs utilizing fibroblast cells from Llama when compared with those reconstructed with dromedary and Bactrian fibroblast cells. However, no difference was observed in their cell numbers. In experiment 2, a higher (P < 0.05) proportion of blastocysts were obtained from the cleaved embryos reconstructed with Bactrian fibroblast cells when compared to those reconstructed with dromedary cells. Twenty-six Day 7 blastocysts reconstructed with Bactrian cells were transferred to 23 synchronized dromedary recipients with 5 pregnancies established on Day 30, however, only one of the pregnancies developed to term and a healthy calf weighing 33 kgs was born after completing 392 days of gestation. Unfortunately, the calf died on day 7 due to acute septicemia. In conclusion, the present study reports, for the first time, birth of a cloned Bactrian calf by iSCNT using dromedary camel as a source for oocytes as well as a surrogate for carrying the pregnancy to term.
[Mh] Termos MeSH primário: Clonagem de Organismos/veterinária
Transferência Embrionária/veterinária
Técnicas de Transferência Nuclear/veterinária
Indução da Ovulação/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Camelídeos Americanos
Camelus
Técnicas de Cultura Embrionária
Espécies em Perigo de Extinção
Feminino
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177800


  7 / 5226 MEDLINE  
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[PMID]:28475629
[Au] Autor:Wang Y; Ding F; Wang T; Liu W; Lindquist S; Hernell O; Wang J; Li J; Li L; Zhao Y; Dai Y; Li N
[Ad] Endereço:State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, P. R. China.
[Ti] Título:Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows.
[So] Source:PLoS One;12(5):e0176864, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for gestational age preterm infants. Thus, purified recombinant human BSSL (rhBSSL) can be used for treatment of patients with fat malabsorption and expressing rhBSSL in the milk of transgenic cloned cows would therefore be a mean to meet a medical need. In the present study, a vector pBAC-hLF-hBSSL was constructed, which efficiently expressed active rhBSSL in milk of transgenic cloned cows to a concentration of 9.8 mg/ml. The rhBSSL purified from cow milk had the same enzymatic activity, N-terminal amino acid sequence, amino acid composition and isoelectric point and similar physicochemical characteristics as human native BSSL. Our study supports the use of transgenic cattle for the cost-competitive, large-scale production of therapeutic rhBSSL.
[Mh] Termos MeSH primário: Lipase/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Western Blotting
Bovinos
Clonagem de Organismos
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Vetores Genéticos
Temperatura Alta
Seres Humanos
Concentração de Íons de Hidrogênio
Cinética
Lipase/química
Lipase/genética
Lipase/uso terapêutico
Síndromes de Malabsorção/tratamento farmacológico
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.1.3 (CEL protein, human); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176864


  8 / 5226 MEDLINE  
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[PMID]:28407864
[Au] Autor:Kuwayama H; Tanabe Y; Wakayama T; Kishigami S
[Ad] Endereço:Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan.
[Ti] Título:Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer.
[So] Source:Theriogenology;94:79-85, 2017 May.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Less invasive methods for donor cell collection will facilitate reproduction of wild animals using somatic-cell nuclear transfer. Stages of the estrous cycle in mice have long been studies using somatic cells that can be collected from vaginal walls using cotton tipped swabs in a relatively non-invasive manner. In this study, we examined the feasibility of these cells as sources of nuclei for somatic-cell cloning using nuclear transfer. Estrous cycles generally comprise proestrus, estrus, metestrus, and diestrus stages. In the present experiments, more than 60% of cells were nucleated in vaginal smears from all but the estrus stage. However, after somatic-cell nuclear transfer of cells from proestrus, metestrus, and diestrus stages, 66%, 50%, and 72% of cloned embryos developed to the morula/blastocyst, and cloned female mouse birth rates after embryo transfer were 1.5%, 0.3%, and 1%, respectively. These results show that noninvasively collected vaginal smears contain somatic cells that can be used to clone female mice.
[Mh] Termos MeSH primário: Clonagem de Organismos
Camundongos/embriologia
Técnicas de Transferência Nuclear/veterinária
Esfregaço Vaginal/veterinária
[Mh] Termos MeSH secundário: Animais
Coeficiente de Natalidade
Transferência Embrionária/veterinária
Desenvolvimento Embrionário
Estro
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE


  9 / 5226 MEDLINE  
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[PMID]:28406938
[Au] Autor:Mun SE; Sim BW; Yoon SB; Jeong PS; Yang HJ; Choi SA; Park YH; Kim YH; Kang P; Jeong KJ; Lee Y; Jin YB; Song BS; Kim JS; Huh JW; Lee SR; Choo YK; Kim SU; Chang KT
[Ad] Endereço:National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Republic of Korea.
[Ti] Título:Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs.
[So] Source:PLoS One;12(4):e0175427, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embryos highly depends on the combination of FBS supplementation timing and embryonic reactive oxygen species (ROS) requirements. Interestingly, FBS significantly reduced intracellular ROS levels in parthenogenetically activated (PA) embryos regardless of the developmental stage. However, the beneficial effect of FBS on early embryogenesis was found only during the late phase (IVC 4-6 days) treatment group. In particular, developmental competence parameters, such as blastocyst formation rate, cellular survival, total cell number and trophectoderm proportion, were markedly increased by FBS supplementation during the late IVC phase. In addition, treatment with FBS elevated antioxidant transcript levels during the late IVC phase. In contrast, supplementation with FBS during the entire period (1-6 days) or during the early IVC phase (1-2 days) greatly impaired the developmental parameters. Consistent with the results from PA embryos, the developmental competence of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) embryos were markedly improved by treatment with FBS during the late IVC phase. Moreover, the embryonic stage-specific effects of FBS were reversed by the addition of an oxidant and were mimicked by treatment with an antioxidant. These findings may increase our understanding of redox-dependent early embryogenesis and contribute to the large-scale production of high-quality IVP embryos.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Técnicas de Cultura Embrionária/métodos
Embrião de Mamíferos/metabolismo
Desenvolvimento Embrionário/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
Soro
[Mh] Termos MeSH secundário: Animais
Bovinos
Clonagem de Organismos/métodos
Partenogênese
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175427


  10 / 5226 MEDLINE  
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[PMID]:28359974
[Au] Autor:Liang Z; Li G; An T
[Ad] Endereço:State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Purifying, cloning and characterizing a novel dehalogenase from Bacillus sp. GZT to enhance the biodegradation of 2,4,6-tribromophenol in water.
[So] Source:Environ Pollut;225:104-111, 2017 Jun.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:2,4,6-Tribromophenol (TBP), an intermediate of brominated flame retardants, can easily release to environment and recalcitrant to degradation. Previously, Bacillus sp. GZT, a pure aerobic strain capable of simultaneously debrominating and mineralizing TBP, was successfully isolated by us. To further obtain a practical application and dig up its TBP degradation mechanism, a total of 46.7-fold purification of a novel dehalogenase with a final specific activity of 18.9 U mg and a molecular mass of 63.4 kDa was achieved. Under optimal conditions (35 °C and 200 rpm), up to 80% degradation efficiencies were achieved within 120 min. Adding H O , NADPH, Mn and Mg promoted enzyme reaction effectively; while EDTA, methyl viologen, Ni , Cu , Ca and Fe strongly inhibited reaction activities. The debromination of TBP was catalyzed by the enzyme at a Km of 78 µM and a Vmax of 0.65 min mg protein , which indicated that this dehalogenase could specifically eliminate TBP with a high efficiency and stability. Based on MALDI-TOF/TOF analysis, the dehalogenase shared 98% identity with peptide ABC transporter substrate-binding protein. One open reading frame (ORF) encoding this peptide was found in Strain GZT genome, subjected to clone and expressed in Escherichia coli (E. coli) to characterize the encoding gene. Result showed that this recombinant strain could also remove as similar amount of TBP as Bacillus sp. GZT under the identical condition. Based on these results, we suggest that this newly-isolated TBP dehalogenase highlights a new approach for remediating TBP pollution.
[Mh] Termos MeSH primário: Bacillus/enzimologia
Proteínas de Bactérias/genética
Biodegradação Ambiental
Fenóis/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacillus/genética
Bacillus/metabolismo
Clonagem de Organismos
Escherichia coli/genética
Halogenação
Peróxido de Hidrogênio/metabolismo
Dados de Sequência Molecular
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Phenols); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); BBX060AN9V (Hydrogen Peroxide); YS6K3EU393 (2,4,6-tribromophenol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE



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