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[PMID]:28470335
[Au] Autor:Han GG; Song AA; Kim EB; Yoon SH; Bok JD; Cho CS; Kil DY; Kang SK; Choi YJ
[Ad] Endereço:Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Improved antimicrobial activity of Pediococcus acidilactici against Salmonella Gallinarum by UV mutagenesis and genome shuffling.
[So] Source:Appl Microbiol Biotechnol;101(13):5353-5363, 2017 Jul.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pediococcus acidilactici is a widely used probiotic, and Salmonella enterica serovar Gallinarum (SG) is a significant pathogen in the poultry industry. In this study, we improved the antimicrobial activity of P. acidilactici against SG using UV mutation and genome shuffling (GS). To improve antimicrobial activity against SG, UV mutagenesis was performed against wild-type P. acidilactici (WT), and five mutants showed improved antimicrobial activity. To further improve antimicrobial activity, GS was performed on five UV mutants. Following GS, four mutants showed improved antimicrobial activity compared with the UV mutants and WT. The antimicrobial activity of GS1 was highest among the mutants; however, the activity was reduced when the culture supernatant was treated with proteinase K, suggesting that the improved antimicrobial activity is due to a proteinous substance such as bacteriocin. To validate the activity of GS1 in vivo, we designed multi-species probiotics and performed broiler feeding experiments. Groups consisted of no treatment (NC), avilamycin-treated (PC), probiotic group 1 containing WT (T1), and probiotic group 2 containing GS1 (T2). In broiler feeding experiments, coliform bacteria were significantly reduced in T2 compared with NC, PC, and T1. The cecal microbiota was modulated and pathogenic bacteria were reduced by GS1 oral administration. In this study, GS1 showed improved antimicrobial activity against SG in vitro and reduced pathogenic bacteria in a broiler feeding experiment. These results suggest that GS1 can serve as an efficient probiotic, as an alternative to antibiotics in the poultry industry.
[Mh] Termos MeSH primário: Antibiose
Embaralhamento de DNA
Mutagênese
Pediococcus acidilactici/genética
Pediococcus acidilactici/fisiologia
Probióticos
Salmonella/fisiologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Anti-Infecciosos
Bacteriocinas/biossíntese
Bacteriocinas/farmacologia
Ceco/microbiologia
Galinhas/microbiologia
Meios de Cultura/química
Endopeptidase K/metabolismo
Genoma Bacteriano
Pediococcus acidilactici/efeitos dos fármacos
Pediococcus acidilactici/efeitos da radiação
Doenças das Aves Domésticas/microbiologia
Doenças das Aves Domésticas/terapia
Probióticos/química
Salmonella/efeitos dos fármacos
Salmonelose Animal/microbiologia
Salmonelose Animal/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Bacteriocins); 0 (Culture Media); EC 3.4.21.64 (Endopeptidase K)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8293-6


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[PMID]:28280866
[Au] Autor:Capel C; Yuste-Lisbona FJ; López-Casado G; Angosto T; Heredia A; Cuartero J; Fernández-Muñoz R; Lozano R; Capel J
[Ad] Endereço:Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, 04120, Almería, Spain.
[Ti] Título:QTL mapping of fruit mineral contents provides new chances for molecular breeding of tomato nutritional traits.
[So] Source:Theor Appl Genet;130(5):903-913, 2017 May.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Agronomical characterization of a RIL population for fruit mineral contents allowed for the identification of QTL controlling these fruit quality traits, flanked by co-dominant markers useful for marker-assisted breeding. Tomato quality is a multi-variant attribute directly depending on fruit chemical composition, which in turn determines the benefits of tomato consumption for human health. Commercially available tomato varieties possess limited variability in fruit quality traits. Wild species, such as Solanum pimpinellifolium, could provide different nutritional advantages and can be used for tomato breeding to improve overall fruit quality. Determining the genetic basis of the inheritance of all the traits that contribute to tomato fruit quality will increase the efficiency of the breeding program necessary to take advantage of the wild species variability. A high-density linkage map has been constructed from a recombinant inbred line (RIL) population derived from a cross between tomato Solanum lycopersicum and the wild-relative species S. pimpinellifolium. The RIL population was evaluated for fruit mineral contents during three consecutive growing seasons. The data obtained allowed for the identification of main QTL and novel epistatic interaction among QTL controlling fruit mineral contents on the basis of a multiple-environment analysis. Most of the QTL were flanked by candidate genes providing valuable information for both tomato breeding for new varieties with novel nutritional properties and the starting point to identify the genes underlying these QTL, which will help to reveal the genetic basis of tomato fruit nutritional properties.
[Mh] Termos MeSH primário: Embaralhamento de DNA
Frutas/química
Lycopersicon esculentum/genética
Locos de Características Quantitativas
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Cromossomos de Plantas
Cruzamentos Genéticos
Epistasia Genética
Ligação Genética
Minerais/análise
Valor Nutritivo
Melhoramento Vegetal
Solanum/genética
Oligoelementos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Minerals); 0 (Trace Elements)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2859-7


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[PMID]:28130668
[Au] Autor:Lumme J; Zietara MS; Lebedeva D
[Ad] Endereço:Department of Biology, University of Oulu, 90014, Oulu, Finland.
[Ti] Título:Ancient and modern genome shuffling: Reticulate mito-nuclear phylogeny of four related allopatric species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae), ectoparasites on the Eurasian minnow Phoxinus phoxinus (L.) (Cyprinidae).
[So] Source:Syst Parasitol;94(2):183-200, 2017 Feb.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phylogenetic analyses including four allopatric species of Gyrodactylus von Nordmann, 1832 on the Eurasian minnow Phoxinus phoxinus (L.) (Cyprinidae) revealed incongruence between the nuclear ITS1-5.8S-ITS2 and mitochondrial cox1 phylogenies due to ancient hybridisation. Gyrodactylus pannonicus Molnár, 1968 was sampled close to its type-locality, the upper reaches of River Tisza, tributary of Danube in the Black Sea Basin. Faunistic search detected three new related species with maximum composite likelihood distances in cox1 between 16.8-23.2% (tentatively 1.3 to 1.8 My of divergence). Gyrodactylus albolacustris n. sp. recorded in the White Sea Basin, eastern Baltic Basin and Mongolia was close to G. pannonicus in the nuclear ITS (divergence of 0.9%), but diverged in cox1 by 19.8%. The Mongolian isolate of G. albolacustris n. sp. diverged from the European isolates in cox1 by 8.9%, suggesting 0.7 My of isolation. The two other new species differed from G. pannonicus by >4% in ITS and some large indels in ITS1, and by >20% in cox1. Gyrodactylus danastriae n. sp. was found in River Strwiaz, a tributary of the River Dniester (Black Sea Basin) and was characterised by smaller size of anchors and by 29-41 bp dimorphic insertion in ITS1. Gyrodactylus botnicus n. sp. is considered endemic in the Baltic Basin, but was also found in the White Sea Basin as a postglacial immigrant, where it had hybridised with G. albolacustris n. sp. in spite of the high divergence in ITS (3.9%) and cox1 (22%). The discordant nuclear and mitochondrial phylogenies revealed an ancient mitochondrial introgression: G. albolacustris n. sp. was derived from a hybridisation combining proto-pannonicus ITS with proto-danastriae mitochondria, perhaps 1.3 My ago. The postglacial hybridisation of G. albolacustris n. sp. (as the donor of mtDNA and ITS ) and G. botnicus n. sp. (donor of the ITS ) offered a model of shuffling of the genomic components: the process of the homogenisation and stabilisation of nuclear ITS (concerted evolution) and the lineage sorting has hardly begun.
[Mh] Termos MeSH primário: Embaralhamento de DNA
Filogenia
Trematódeos/classificação
Trematódeos/genética
[Mh] Termos MeSH secundário: Animais
Cyprinidae/parasitologia
DNA Mitocondrial/genética
DNA Espaçador Ribossômico/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-016-9696-y


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[PMID]:28126817
[Au] Autor:Tieman D; Zhu G; Resende MF; Lin T; Nguyen C; Bies D; Rambla JL; Beltran KS; Taylor M; Zhang B; Ikeda H; Liu Z; Fisher J; Zemach I; Monforte A; Zamir D; Granell A; Kirst M; Huang S; Klee H
[Ad] Endereço:Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, No. 7, Pengfei Road, Dapeng District, Shenzhen 518124, China.
[Ti] Título:A chemical genetic roadmap to improved tomato flavor.
[So] Source:Science;355(6323):391-394, 2017 01 27.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Modern commercial tomato varieties are substantially less flavorful than heirloom varieties. To understand and ultimately correct this deficiency, we quantified flavor-associated chemicals in 398 modern, heirloom, and wild accessions. A subset of these accessions was evaluated in consumer panels, identifying the chemicals that made the most important contributions to flavor and consumer liking. We found that modern commercial varieties contain significantly lower amounts of many of these important flavor chemicals than older varieties. Whole-genome sequencing and a genome-wide association study permitted identification of genetic loci that affect most of the target flavor chemicals, including sugars, acids, and volatiles. Together, these results provide an understanding of the flavor deficiencies in modern commercial varieties and the information necessary for the recovery of good flavor through molecular breeding.
[Mh] Termos MeSH primário: Lycopersicon esculentum/química
Lycopersicon esculentum/genética
Melhoramento Vegetal
Paladar
[Mh] Termos MeSH secundário: Alelos
Carboidratos/genética
Cromossomos de Plantas/genética
Embaralhamento de DNA
Loci Gênicos
Estudo de Associação Genômica Ampla
Cetonas/química
Compostos Orgânicos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Ketones); 0 (Volatile Organic Compounds); 448353S93V (methylheptenone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1126/science.aal1556


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[PMID]:28096780
[Au] Autor:Manoharan RK; Jung HJ; Hwang I; Jeong N; Kho KH; Chung MY; Nou IS
[Ad] Endereço:Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon, Jeonnam 57922 Republic of Korea.
[Ti] Título:Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation.
[So] Source:Hereditas;154:1, 2017.
[Is] ISSN:1601-5223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tomatoes provide a significant dietary source of the carotenoids, lycopene and ß-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties. METHODS: We crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel 'orange-brown' fruit color was identified. RESULTS: We confirm two SNPs, one in and another in gene sequence, associated with segregation of 'orange-brown' fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high ß-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.
[Mh] Termos MeSH primário: Clorofila/química
Embaralhamento de DNA
Frutas/química
Lycopersicon esculentum/genética
beta Caroteno/química
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Fenótipo
Melhoramento Vegetal
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
01YAE03M7J (beta Carotene); 1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1186/s41065-016-0023-z


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[PMID]:28074224
[Au] Autor:Gu C; Wang G; Mai S; Wu P; Wu J; Wang G; Liu H; Zhang J
[Ad] Endereço:Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
[Ti] Título:ARTP mutation and genome shuffling of ABE fermentation symbiotic system for improvement of butanol production.
[So] Source:Appl Microbiol Biotechnol;101(5):2189-2199, 2017 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Butanol is an ideal renewable biofuel which possesses superior fuel properties. Previously, butanol-producing symbiotic system TSH06 was isolated in our lab, with microoxygen tolerance ability. To boost butanol yield for large-scale industrial production, TSH06 was used as parental strain and subjected to atmospheric and room temperature plasma (ARTP) and four rounds of genome shuffling (GS). ARTP mutant and GS strain were co-cultured with facultative anaerobic Bacillus cereus TSH2 to form a symbiotic system with microoxygen tolerance, which was then subjected to fermentation. Relative messenger RNA (mRNA) level of key enzyme gene was measured by real-time PCR. The highest butanol titer of TS4-30 reached 15.63 g/L, which was 34% higher than TSH06 (12.19 g/L). Compared with parental strain, mRNA of acid-forming gene in TS4-30 decreased in acidogenesis phase, while solvent-forming gene increased in solventogenesis phase. This gene expression pattern was consistent with high butanol yield and low acid level in TS4-30. In summary, symbiotic system TS4-30 was obtained with butanol titer improvement and microoxygen tolerance.
[Mh] Termos MeSH primário: Bacillus cereus/metabolismo
Biocombustíveis/microbiologia
Butanóis/metabolismo
Clostridium acetobutylicum/metabolismo
Embaralhamento de DNA
[Mh] Termos MeSH secundário: Anaerobiose/genética
Fermentação
Biblioteca Gênica
Mutação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Butanols)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8093-z


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[PMID]:28004139
[Au] Autor:Lee BU; Choi MS; Kim DM; Oh KH
[Ad] Endereço:Division of Biological Sciences and Chemistry, Kosin University, Pusan, 49104, Republic of Korea.
[Ti] Título:Genome Shuffling of Stenotrophomonas maltophilia OK-5 for Improving the Degradation of Explosive RDX (Hexahydro-1,3,5-trinitro-1,3,5-triazine).
[So] Source:Curr Microbiol;74(2):268-276, 2017 Feb.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A genome-shuffled Stenotrophomonas maltophilia strain showing the enhanced ability of RDX degradation was constructed, and its characteristics were compared with those of the wild-type one. The shuffled strain was able to completely degrade 25, 50, and 75 µM RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) within 10, 30, and 50 days, respectively. However, it took 30 and 70 days for the wild-type strain to degrade 25 and 50 µM RDX, respectively, and at day 70, the strain degraded only 67% of 75 µM RDX. The shuffled strain reached its maximum growth at 50-60 days and exhibited approximately 1.5-fold increased cell numbers. SEM revealed more severe damage on the surface of the wild-type cells compared to the genome-shuffled cells. The mRNA levels of dnaK and groEL encoding the heat shock proteins were increased by 2.5-fold and fourfold, and DnaK and GroEL proteins were more highly produced in the shuffled cells. In addition, the mRNA levels of pnrB encoding a TNT nitroreductase, and algA involved in exopolymer biosynthesis, were slightly higher in the shuffled strain, but not as high as those of dnaK and groEL. These results indicate that the genome shuffling rendered the shuffled cells more resistant to RDX stress. A proteomic comparison revealed changes in the production levels of certain proteins including nitrate and cell protection, particularly those involved in metabolism. These proteomic analyses provide clues for understanding the improved RDX degradation by the genome-shuffled S. maltophilia strain.
[Mh] Termos MeSH primário: Embaralhamento de DNA
Substâncias Explosivas/metabolismo
Genoma Bacteriano
Stenotrophomonas maltophilia/genética
Stenotrophomonas maltophilia/metabolismo
Triazinas/metabolismo
[Mh] Termos MeSH secundário: Biotransformação
Perfilação da Expressão Gênica
Redes e Vias Metabólicas/genética
Microscopia Eletrônica de Varredura
Proteoma/análise
Stenotrophomonas maltophilia/ultraestrutura
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Explosive Agents); 0 (Proteome); 0 (Triazines); W91SSV5831 (cyclonite)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1179-5


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[PMID]:27853895
[Au] Autor:Wang S; Duan M; Liu Y; Fan S; Lin X; Zhang Y
[Ad] Endereço:School of Bioscience and Bioengineering, Guangzhou Higher Education Mega Centre, South China University of Technology, Panyu District, Guangzhou, 510006, China.
[Ti] Título:Enhanced production of fructosyltransferase in Aspergillus oryzae by genome shuffling.
[So] Source:Biotechnol Lett;39(3):391-396, 2017 Mar.
[Is] ISSN:1573-6776
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To breed Aspergillus oryzae strains with high fructosyltransferase (FTase) activity using intraspecific protoplast fusion via genome-shuffling. RESULTS: A candidate library was developed using UV/LiCl of the conidia of A. oryzae SBB201. By screening for enzyme activity and cell biomass, two mutants (UV-11 and UV-76) were chosen for protoplast fusion and subsequent genome shuffling. After three rounds of genome recombination, a fusion mutant RIII-7 was obtained. Its FTase activity was 180 U g , approximately double that of the original strain, and RIII-7 was genetically stable. In fermentation culture, FTase activity of the genome-shuffled strain reached a maximum of 353 U g using substrate-feeding method, and this value was approximately 3.4-times higher than that of the original strain A. oryzae SBB201. CONCLUSIONS: Intraspecific protoplast fusion of A. oryzae significantly enhanced FTase activity and generated a potentially useful strain for industrial production.
[Mh] Termos MeSH primário: Aspergillus oryzae/enzimologia
Embaralhamento de DNA/métodos
Genoma Fúngico
Hexosiltransferases/biossíntese
[Mh] Termos MeSH secundário: Aspergillus oryzae/efeitos dos fármacos
Fermentação/efeitos dos fármacos
Instabilidade Genômica
Fusão de Membrana/efeitos dos fármacos
Mutagênese/genética
Mutação/genética
Protoplastos/efeitos dos fármacos
Protoplastos/metabolismo
Regeneração/efeitos dos fármacos
Sacarose/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
57-50-1 (Sucrose); EC 2.4.1.- (Hexosyltransferases); EC 2.4.1.9 (inulosucrase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1007/s10529-016-2254-5


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[PMID]:27691905
[Au] Autor:Zhao Y; Duan C; Gao L; Yu X; Niu C; Li S
[Ad] Endereço:a Institute of Agro-food Technology , Jilin Academy of Agricultural Sciences , Changchun , PR China.
[Ti] Título:Genome shuffling of Lactobacillus plantarum C88 improves adhesion.
[So] Source:Biosci Biotechnol Biochem;81(1):184-193, 2017 Jan.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Genome shuffling is an important method for rapid improvement in microbial strains for desired phenotypes. In this study, ultraviolet irradiation and nitrosoguanidine were used as mutagens to enhance the adhesion of the wild-type Lactobacillus plantarum C88. Four strains with better property were screened after mutagenesis to develop a library of parent strains for three rounds of genome shuffling. Fusants F3-1, F3-2, F3-3, and F3-4 were screened as the improved strains. The in vivo and in vitro tests results indicated that the population after three rounds of genome shuffling exhibited improved adhesive property. Random Amplified Polymorphic DNA results showed significant differences between the parent strain and recombinant strains at DNA level. These results suggest that the adhesive property of L. plantarum C88 can be significantly improved by genome shuffling. Improvement in the adhesive property of bacterial cells by genome shuffling enhances the colonization of probiotic strains which further benefits to exist probiotic function.
[Mh] Termos MeSH primário: Aderência Bacteriana/genética
Embaralhamento de DNA
Genoma Bacteriano/genética
Lactobacillus plantarum/genética
Lactobacillus plantarum/fisiologia
[Mh] Termos MeSH secundário: Ácidos e Sais Biliares/metabolismo
Interações Hidrofóbicas e Hidrofílicas
Lactobacillus plantarum/metabolismo
Viabilidade Microbiana
Mutagênese
Polimorfismo Genético
Probióticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bile Acids and Salts)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:27676587
[Au] Autor:Luna-Flores CH; Palfreyman RW; Krömer JO; Nielsen LK; Marcellin E
[Ad] Endereço:Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Qld, Australia.
[Ti] Título:Improved production of propionic acid using genome shuffling.
[So] Source:Biotechnol J;12(2), 2017 Feb.
[Is] ISSN:1860-7314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Traditionally derived from fossil fuels, biological production of propionic acid has recently gained interest. Propionibacterium species produce propionic acid as their main fermentation product. Production of other organic acids reduces propionic acid yield and productivity, pointing to by-products gene-knockout strategies as a logical solution to increase yield. However, removing by-product formation has seen limited success due to our inability to genetically engineer the best producing strains (i.e. Propionibacterium acidipropionici). To overcome this limitation, random mutagenesis continues to be the best path towards improving strains for biological propionic acid production. Recent advances in next generation sequencing opened new avenues to understand improved strains. In this work, we use genome shuffling on two wild type strains to generate a better propionic acid producing strain. Using next generation sequencing, we mapped the genomic changes leading to the improved phenotype. The best strain produced 25% more propionic acid than the wild type strain. Sequencing of the strains showed that genomic changes were restricted to single point mutations and gene duplications in well-conserved regions in the genomes. Such results confirm the involvement of gene conversion in genome shuffling as opposed to long genomic insertions.
[Mh] Termos MeSH primário: Biotecnologia/métodos
Embaralhamento de DNA
Propionatos/metabolismo
[Mh] Termos MeSH secundário: Sequenciamento de Nucleotídeos em Larga Escala
Propionibacterium/genética
Propionibacterium/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Propionates); JHU490RVYR (propionic acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160928
[St] Status:MEDLINE
[do] DOI:10.1002/biot.201600120



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