Base de dados : MEDLINE
Pesquisa : E05.393.420.270 [Categoria DeCS]
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  1 / 775 MEDLINE  
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[PMID]:29219994
[Ti] Título:Gene-drive technology needs thorough scrutiny.
[So] Source:Nature;552(7683):6, 2017 12 07.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Tecnologia de Impulso Genético/efeitos adversos
Tecnologia de Impulso Genético/legislação & jurisprudência
Edição de Genes/legislação & jurisprudência
Medição de Risco
Nações Unidas/legislação & jurisprudência
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Canadá
Congressos como Assunto
Correio Eletrônico
Cooperação Internacional
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1038/d41586-017-08214-4


  2 / 775 MEDLINE  
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[PMID]:29231142
[Au] Autor:Duardo-Sanchez A
[Ad] Endereço:Department of Public Law, Law and the Human Genome Research Group, University of the Basque Country UPV/EHU, 48940, Leioa, Biscay, Spain.
[Ti] Título:CRISPR-Cas in Medicinal Chemistry: Applications and Regulatory Concerns.
[So] Source:Curr Top Med Chem;17(30):3308-3315, 2017.
[Is] ISSN:1873-4294
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A rapid search in scientific publication's databases shows how the use of CRISPR-Cas genome editions' technique has considerably expanded, and its growing importance, in modern molecular biology. Just in pub-med platform, the search of the term gives more than 3000 results. Specifically, in Drug Discovery, Medicinal Chemistry and Chemical Biology in general CRISPR method may have multiple applications. Some of these applications are: resistance-selection studies of antimalarial lead organic compounds; investigation of druggability; development of animal models for chemical compounds testing, etc. In this paper, we offer a review of the most relevant scientific literature illustrated with specific examples of application of CRISPR technique to medicinal chemistry and chemical biology. We also present a general overview of the main legal and ethical trends regarding this method of genome editing.
[Mh] Termos MeSH primário: Química Farmacêutica
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas/genética
Edição de Genes/ética
Edição de Genes/legislação & jurisprudência
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.2174/1568026618666171211151142


  3 / 775 MEDLINE  
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[PMID]:29254925
[Au] Autor:Huang JJ; Cao CW; Zheng GM; Zhao JG
[Ad] Endereço:State Key Lab of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:Genome editing technologies drive the development of pig genetic improvement.
[So] Source:Yi Chuan;39(11):1078-1089, 2017 Nov 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Nuclease-mediated genome editing technologies contribute to the rapid advances in life sciences via the ability to edit the genomes within living cells, and present a new era for porcine genetic improvement. In this review, we introduce the development of various genomic editing technologies, particularly CRISPR/Cas9 strategies and characteristics of various naturally occurring and artificially engineered CRISPR enzymes. Also, we summarize progress in pig genetic improvement mediated by genome editing, especially those associated with meat quality traits and anti-virus resistance. We highlight the challenges in the implementation of pig genetic improvement and the prospects of pig genetic breeding based on genome editing technologies.
[Mh] Termos MeSH primário: Edição de Genes/métodos
Suínos/genética
[Mh] Termos MeSH secundário: Animais
Cruzamento
Sistemas CRISPR-Cas
Engenharia Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.16288/j.yczz.17-130


  4 / 775 MEDLINE  
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[PMID]:29254920
[Au] Autor:Ma SY; Xia QY
[Ad] Endereço:State Key Laboratory of Silkworm Genome Biology, Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400716, China.
[Ti] Título:Genetic breeding of silkworms: from traditional hybridization to molecular design.
[So] Source:Yi Chuan;39(11):1025-1032, 2017 Nov 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Sericulture is one of the great inventions of the Chinese people and has become an important cultural feature of China. As China is the long-lasting center of silk production, genetic breeding of silkworm was highly developed historically, and has formed a comprehensive system for breeding and preservation of new varieties. However, silkworm breeding reached a bottleneck recently, because most of the traditional genetic resources have been utilized and silkworm strains have become homogeneous. Meanwhile, sericulture in China meets huge challenges in the 21 century. In recent years, with the development and rapid application of molecular biology, genomics, transgene and genome editing, silkworm genetic breeding has entered a new era. In this review, we summarize the development of silkworm genetic breeding, especially the progress and perspective of transgene and genome editing in genetic engineering of silkworms. We also discuss the future development of silkworm genetic breeding.
[Mh] Termos MeSH primário: Bombyx/genética
Cruzamento
[Mh] Termos MeSH secundário: Animais
Edição de Genes
Hibridização Genética
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.16288/j.yczz.17-103


  5 / 775 MEDLINE  
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[PMID]:29379011
[Au] Autor:Yoon Y; Wang D; Tai PWL; Riley J; Gao G; Rivera-Pérez JA
[Ad] Endereço:Department of Pediatrics, Division of Genes and Development, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
[Ti] Título:Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses.
[So] Source:Nat Commun;9(1):412, 2018 01 29.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent advances using CRISPR-Cas9 approaches have dramatically enhanced the ease for genetic manipulation in rodents. Notwithstanding, the methods to deliver nucleic acids into pre-implantation embryos have hardly changed since the original description of mouse transgenesis more than 30 years ago. Here we report a novel strategy to generate genetically modified mice by transduction of CRISPR-Cas9 components into pre-implantation mouse embryos via recombinant adeno-associated viruses (rAAVs). Using this approach, we efficiently generated a variety of targeted mutations in explanted embryos, including indel events produced by non-homologous end joining and tailored mutations using homology-directed repair. We also achieved gene modification in vivo by direct delivery of rAAV particles into the oviduct of pregnant females. Our approach greatly simplifies the generation of genetically modified mice and, more importantly, opens the door for streamlined gene editing in other mammalian species.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Dependovirus/genética
Desenvolvimento Embrionário/genética
Edição de Genes/métodos
Engenharia Genética/métodos
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Blastocisto
Reparo do DNA por Junção de Extremidades
Dependovirus/metabolismo
Endonucleases/genética
Endonucleases/metabolismo
Tubas Uterinas/embriologia
Feminino
Genes Reporter
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Células HEK293
Seres Humanos
Camundongos
Camundongos Transgênicos
Mutagênese Sítio-Dirigida
Gravidez
Reparo de DNA por Recombinação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); EC 3.1.- (Cas9 endonuclease Streptococcus pyogenes); EC 3.1.- (Endonucleases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02706-7


  6 / 775 MEDLINE  
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[PMID]:29267279
[Au] Autor:Cheung NKM; Nakamura R; Uno A; Kumagai M; Fukushima HS; Morishita S; Takeda H
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
[Ti] Título:Unlinking the methylome pattern from nucleotide sequence, revealed by large-scale in vivo genome engineering and methylome editing in medaka fish.
[So] Source:PLoS Genet;13(12):e1007123, 2017 12.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The heavily methylated vertebrate genomes are punctuated by stretches of poorly methylated DNA sequences that usually mark gene regulatory regions. It is known that the methylation state of these regions confers transcriptional control over their associated genes. Given its governance on the transcriptome, cellular functions and identity, genome-wide DNA methylation pattern is tightly regulated and evidently predefined. However, how is the methylation pattern determined in vivo remains enigmatic. Based on in silico and in vitro evidence, recent studies proposed that the regional hypomethylated state is primarily determined by local DNA sequence, e.g., high CpG density and presence of specific transcription factor binding sites. Nonetheless, the dependency of DNA methylation on nucleotide sequence has not been carefully validated in vertebrates in vivo. Herein, with the use of medaka (Oryzias latipes) as a model, the sequence dependency of DNA methylation was intensively tested in vivo. Our statistical modeling confirmed the strong statistical association between nucleotide sequence pattern and methylation state in the medaka genome. However, by manipulating the methylation state of a number of genomic sequences and reintegrating them into medaka embryos, we demonstrated that artificially conferred DNA methylation states were predominantly and robustly maintained in vivo, regardless of their sequences and endogenous states. This feature was also observed in the medaka transgene that had passed across generations. Thus, despite the observed statistical association, nucleotide sequence was unable to autonomously determine its own methylation state in medaka in vivo. Our results apparently argue against the notion of the governance on the DNA methylation by nucleotide sequence, but instead suggest the involvement of other epigenetic factors in defining and maintaining the DNA methylation landscape. Further investigation in other vertebrate models in vivo will be needed for the generalization of our observations made in medaka.
[Mh] Termos MeSH primário: Metilação de DNA
Oryzias/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Ilhas de CpG
DNA/genética
Epigênese Genética
Epigenômica/métodos
Evolução Molecular
Edição de Genes
Regulação da Expressão Gênica
Genoma
Oryzias/metabolismo
Regiões Promotoras Genéticas
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007123


  7 / 775 MEDLINE  
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[PMID]:29261646
[Au] Autor:Hamm DC; Larson ED; Nevil M; Marshall KE; Bondra ER; Harrison MM
[Ad] Endereço:Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America.
[Ti] Título:A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster.
[So] Source:PLoS Genet;13(12):e1007120, 2017 12.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In nearly all metazoans, the earliest stages of development are controlled by maternally deposited mRNAs and proteins. The zygotic genome becomes transcriptionally active hours after fertilization. Transcriptional activation during this maternal-to-zygotic transition (MZT) is tightly coordinated with the degradation of maternally provided mRNAs. In Drosophila melanogaster, the transcription factor Zelda plays an essential role in widespread activation of the zygotic genome. While Zelda expression is required both maternally and zygotically, the mechanisms by which it functions to remodel the embryonic genome and prepare the embryo for development remain unclear. Using Cas9-mediated genome editing to generate targeted mutations in the endogenous zelda locus, we determined the functional relevance of protein domains conserved amongst Zelda orthologs. We showed that neither a conserved N-terminal zinc finger nor an acidic patch were required for activity. Similarly, a previously identified splice isoform of zelda is dispensable for viability. By contrast, we identified a highly conserved zinc-finger domain that is essential for the maternal, but not zygotic functions of Zelda. Animals homozygous for mutations in this domain survived to adulthood, but embryos inheriting these loss-of-function alleles from their mothers died late in embryogenesis. These mutations did not interfere with the capacity of Zelda to activate transcription in cell culture. Unexpectedly, these mutations generated a hyperactive form of the protein and enhanced Zelda-dependent gene expression. These data have defined a protein domain critical for controlling Zelda activity during the MZT, but dispensable for its roles later in development, for the first time separating the maternal and zygotic requirements for Zelda. This demonstrates that highly regulated levels of Zelda activity are required for establishing the developmental program during the MZT. We propose that tightly regulated gene expression is essential to navigate the MZT and that failure to precisely execute this developmental program leads to embryonic lethality.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Herança Materna/genética
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Sistemas CRISPR-Cas
Sequência Conservada
Drosophila melanogaster
Edição de Genes
Regulação da Expressão Gênica no Desenvolvimento
Mutação
Regiões Promotoras Genéticas
Domínios Proteicos
Estabilidade de RNA/genética
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Dedos de Zinco/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (RNA, Messenger); 0 (Transcription Factors); 0 (Zelda protein, Drosophila)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007120


  8 / 775 MEDLINE  
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[PMID]:29377933
[Au] Autor:Sato'o Y; Hisatsune J; Yu L; Sakuma T; Yamamoto T; Sugai M
[Ad] Endereço:Department of Bacteriology, Hiroshima University, Graduate school of Biomedical and Health Sciences, Hiroshima, Hiroshima, Japan.
[Ti] Título:Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference.
[So] Source:PLoS One;13(1):e0185987, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Preparing the genetically modified organisms have required much time and labor, making it the rate-limiting step but CRISPR/Cas9 technology appearance has changed this difficulty. Although reports on CRISPR/Cas9 technology such as genome editing and CRISPR interference (CRISPRi) in eukaryotes increased, those in prokaryotes especially in Staphylococci were limited. Thus, its potential in the bacteriology remains unexplored. This is attributed to ecological difference between eukaryotes and prokaryotes. Here, we constructed a novel CRISPRi plasmid vector, pBACi for Staphylococcus aureus. The transformation efficiency of S. aureus was ~104 CFU/µg DNA using a vector extracted from dcm negative, which encoded one of DNA modification genes, E. coli. Further, pBACi was introduced into various clinical isolates including that not accepting the conventional temperature-sensitive vector. dcas9 in the vector was expressed throughout the growth phases of S. aureus and this vector decreased various gene mRNA expressions based on the crRNA targeting sequences and altered the knockdown strains' phenotypes. The targeted genes included various virulence and antibiotic resistant genes. Bioinformatics suggest this vector can be introduced into wide range of low-GC Gram-positive bacteria. Because this new CRISPR/Cas9-based vector can easily prepare knockdown strains, we believe the novel vector will facilitate the characterization of the function of genes from S. aureus and other Gram-positive bacteria.
[Mh] Termos MeSH primário: Técnicas de Silenciamento de Genes/métodos
Vetores Genéticos/síntese química
Staphylococcus aureus/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sistemas CRISPR-Cas/genética
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Escherichia coli/genética
Edição de Genes
Inativação Gênica
Vetores Genéticos/genética
Genoma Bacteriano
Plasmídeos/genética
RNA Guia/genética
Infecções Estafilocócicas/genética
Staphylococcus aureus/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Guide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185987


  9 / 775 MEDLINE  
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[PMID]:28457885
[Au] Autor:Brunger JM; Zutshi A; Willard VP; Gersbach CA; Guilak F
[Ad] Endereço:Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
[Ti] Título:Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs.
[So] Source:Stem Cell Reports;8(5):1202-1213, 2017 May 09.
[Is] ISSN:2213-6711
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic inflammatory diseases such as arthritis are characterized by dysregulated responses to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α). Pharmacologic anti-cytokine therapies are often effective at diminishing this inflammatory response but have significant side effects and are used at high, constant doses that do not reflect the dynamic nature of disease activity. Using the CRISPR/Cas9 genome-engineering system, we created stem cells that antagonize IL-1- or TNF-α-mediated inflammation in an autoregulated, feedback-controlled manner. Our results show that genome engineering can be used successfully to rewire endogenous cell circuits to allow for prescribed input/output relationships between inflammatory mediators and their antagonists, providing a foundation for cell-based drug delivery or cell-based vaccines via a rapidly responsive, autoregulated system. The customization of intrinsic cellular signaling pathways in stem cells, as demonstrated here, opens innovative possibilities for safer and more effective therapeutic approaches for a wide variety of diseases.
[Mh] Termos MeSH primário: Edição de Genes/métodos
Fatores Imunológicos/genética
Células-Tronco Pluripotentes Induzidas/metabolismo
Transplante de Células-Tronco/métodos
[Mh] Termos MeSH secundário: Animais
Artrite/terapia
Sistemas CRISPR-Cas
Cartilagem/fisiologia
Células Cultivadas
Retroalimentação Fisiológica
Fatores Imunológicos/metabolismo
Células-Tronco Pluripotentes Induzidas/citologia
Células-Tronco Pluripotentes Induzidas/transplante
Interleucina-1/genética
Interleucina-1/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Regeneração
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Interleukin-1); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  10 / 775 MEDLINE  
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[PMID]:28749735
[Au] Autor:Borges AL; Davidson AR; Bondy-Denomy J
[Ad] Endereço:Department of Microbiology and Immunology, University of California, San Francisco, California 94158; email: joseph.bondy-denomy@ucsf.edu.
[Ti] Título:The Discovery, Mechanisms, and Evolutionary Impact of Anti-CRISPRs.
[So] Source:Annu Rev Virol;4(1):37-59, 2017 Sep 29.
[Is] ISSN:2327-0578
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria and archaea use CRISPR-Cas adaptive immune systems to defend themselves from infection by bacteriophages (phages). These RNA-guided nucleases are powerful weapons in the fight against foreign DNA, such as phages and plasmids, as well as a revolutionary gene editing tool. Phages are not passive bystanders in their interactions with CRISPR-Cas systems, however; recent discoveries have described phage genes that inhibit CRISPR-Cas function. More than 20 protein families, previously of unknown function, have been ascribed anti-CRISPR function. Here, we discuss how these CRISPR-Cas inhibitors were discovered and their modes of action were elucidated. We also consider the potential impact of anti-CRISPRs on bacterial and phage evolution. Finally, we speculate about the future of this field.
[Mh] Termos MeSH primário: Bactérias/genética
Bacteriófagos/genética
Bacteriófagos/fisiologia
Sistemas CRISPR-Cas
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Evolução Molecular
[Mh] Termos MeSH secundário: Archaea/genética
Bactérias/virologia
Bacteriófagos/metabolismo
Edição de Genes
Proteínas Virais/genética
Proteínas Virais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180217
[Lr] Data última revisão:
180217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-virology-101416-041616



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