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[PMID]:27777265
[Au] Autor:Gellatly C; Petrie M
[Ad] Endereço:Institute of Health and Society, Newcastle University, Newcastle upon Tyne, UK.
[Ti] Título:Prenatal sex selection and female infant mortality are more common in India after firstborn and second-born daughters.
[So] Source:J Epidemiol Community Health;71(3):269-274, 2017 Mar.
[Is] ISSN:1470-2738
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The Indian sex ratio has become highly male-biased in recent decades. This may be attributed to prenatal sex selection (PSS) and excess female infant mortality. However, the question of whether these factors are related has not been adequately studied. Here we examine whether increased use of PSS may offset excess female infant mortality, by reducing the number of 'unwanted' daughters being born. METHODS: We analyse the National Family Health Survey (NHFS) data sets for India, which contain nationally representative samples of birth histories for women aged 15-49, interviewed in 1992-1993, 1998-1999 and 2005-2006. We test for missing female births at the second and third birth order, by analysis of the frequencies of sibling sex combinations, and examine the mortality differential between male and female infants, controlling for household wealth and sex(es) of older siblings. RESULTS: PSS was used most in wealthier households at the second and third birth order, when the firstborn, or firstborn and second-born, siblings were female. Having preceding female siblings was a significant risk factor for female infant mortality, but was not correlated with household wealth. CONCLUSIONS: PSS and female infant mortality increase with the presence of older female siblings, yet we find no evidence that increasing use of PSS prevents female infant mortality, because PSS and the proportion of female infant mortality attributable to having older sisters increased over the study period. Increased pressure on higher birth order females caused by the trend towards smaller family sizes may explain this.
[Mh] Termos MeSH primário: Mortalidade Infantil
Núcleo Familiar
Pré-Seleção do Sexo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Ordem de Nascimento
Feminino
Seres Humanos
Índia
Lactente
Recém-Nascido
Meia-Idade
Fatores de Risco
Razão de Masculinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1136/jech-2016-207489


  2 / 1223 MEDLINE  
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[PMID]:28373728
[Au] Autor:Colazo MG; Mapletoft RJ
[Ad] Endereço:Livestock Research Branch, Alberta Agriculture and Forestry, Edmonton, Alberta T6H 5T6 (Colazo); Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Mapletoft).
[Ti] Título:Pregnancy per AI in Holstein heifers inseminated with sex-selected or conventional semen after estrus detection or timed-AI.
[So] Source:Can Vet J;58(4):365-370, 2017 Apr.
[Is] ISSN:0008-5286
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:This study compared pregnancy per AI (P/AI) of heifers inseminated with sex-selected or conventional semen after estrus detection (ED) or timed-AI (TAI). Heifers in the ED group received 2 treatments with prostaglandin F2α 14 d apart and those in the TAI group received a modified 5-day Co-synch protocol plus an intravaginal progesterone releasing insert device (PRID) and were inseminated 72 h after PRID removal. Overall P/AI were 69.2% (74/107) and 64.1% (75/117) for conventional and sex-selected semen ( > 0.05). Although P/AI in ED heifers following the use of conventional semen were numerically higher (75.0% 63.6%), P/AI with sex-selected semen were almost identical (65.0% 63.2%) for ED and TAI heifers. Pregnancy losses from first pregnancy diagnosis to term did not differ between ED and TAI heifers (6.0% 11.3%). In summary, heifers subjected to TAI with sex-selected semen following the application of a modified 5-day Co-synch plus PRID protocol had acceptable P/AI.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Detecção do Estro
Inseminação Artificial/veterinária
Prenhez/fisiologia
[Mh] Termos MeSH secundário: Aborto Animal
Animais
Cloprostenol/administração & dosagem
Dinoprosta/administração & dosagem
Feminino
Inseminação Artificial/métodos
Gravidez
Progesterona/administração & dosagem
Pré-Seleção do Sexo/veterinária
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
4208238832 (Cloprostenol); 4G7DS2Q64Y (Progesterone); B7IN85G1HY (Dinoprost)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


  3 / 1223 MEDLINE  
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[PMID]:28285278
[Au] Autor:Neogi SB; Zodpey S; Negandhi P; Gupta R
[Ad] Endereço:Indian Institute of Public Health-Delhi, Public Health Foundation of India, New Delhi, India. Correspondence to: Dr Sutapa B Neogi, Indian Institute of Public Health Delhi, Public Health Foundation of India, Plot number 47, Sector 44, Gurgaon 122 002, Haryana, India. sutapa.bneogi@iiphd.org.
[Ti] Título:Use of Sex Selection Techniques for Social Reasons: A Menace.
[So] Source:Indian Pediatr;54(2):99-101, 2017 Feb 15.
[Is] ISSN:0974-7559
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Sex selection skewed towards males is a malady that our society is grappling with. The desire to have a child of preferred gender has encouraged people to move beyond the ambit of traditions and explore scientific methods. Despite the controversies around sex-selection for social reasons and strong regulatory mechanisms in place, the demand for such measures has not gone down. On the contrary, traditional practice of consuming indigenous medicines during pregnancy for a male child continues. Recent research highlights the harms of this practice in the form of birth defects and stillbirths. This has led to stricter enforcement of PCPNDT Act and has stimulated the propagation of messages on the harms of these practices in the community.
[Mh] Termos MeSH primário: Pré-Seleção do Sexo
[Mh] Termos MeSH secundário: Feminino
História do Século XX
História do Século XXI
Seres Humanos
Índia
Masculino
Gravidez
Pré-Seleção do Sexo/ética
Pré-Seleção do Sexo/história
Pré-Seleção do Sexo/legislação & jurisprudência
Pré-Seleção do Sexo/estatística & dados numéricos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


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[PMID]:28237584
[Au] Autor:Ettema JF; Thomasen JR; Hjortø L; Kargo M; Østergaard S; Sørensen AC
[Ad] Endereço:SimHerd A/S, Agro Business Park, Niels Pedersens Allé 2, 8830 Tjele, Denmark; Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, Blichers Allé 20, PO Box 50, 8830 Tjele, Denmark. Electronic address: je@simherd.com.
[Ti] Título:Economic opportunities for using sexed semen and semen of beef bulls in dairy herds.
[So] Source:J Dairy Sci;100(5):4161-4171, 2017 May.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dairy farmers can increase the number of dairy heifer calves born in their herd by using sexed semen. They can reduce the number of both dairy bull and heifer calves by using beef semen. Long before sexed semen became commercially available, it was believed that it would provide opportunities for increasing genetic level in both herds and populations. In this study, we studied the potential for increasing the genetic level of a herd by using beef semen in combination with sexed semen. We tested the hypothesis that the potential of increasing the genetic level and the overall net return would depend on herd management. To test this hypothesis, we simulated 7 scenarios using beef semen and sexed semen in 5 herds at different management levels. We combined the results of 2 stochastic simulation models, SimHerd and ADAM. SimHerd simulated the effects of the scenarios and management levels on economic outcomes (i.e., operational return) and on technical outcomes such as the parity distribution of the dams of heifer calves, but it disregarded genetic progress. The ADAM model quantified genetic level by using the dams' parity distributions and the frequency of sexed and beef semen to estimate genetic return per year. We calculated the annual net return per slot as the sum of the operational return and the genetic return, divided by the total number of slots. Net return increased up to €18 per slot when using sexed semen in 75% genetically superior heifers and beef semen in 70% genetically inferior, multiparous cows. The assumed reliability of selection was 0.84. These findings were for a herd with overall high management for reproductive performance, longevity, and calf survival. The same breeding strategy reduced net return by €55 per slot when management levels were average. The main reason for the large reduction in net return was the heifer shortage that arose in this scenario. Our hypothesis that the potential for beef semen to increase genetic level would be herd-specific was supported. None of the scenarios were profitable under Danish circumstances when the value of the increased genetic level was not included. A comparable improvement in genetic level could be realized by selectively selling dairy heifer calves rather than using beef semen.
[Mh] Termos MeSH primário: Indústria de Laticínios
Sêmen
Pré-Seleção do Sexo/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Inseminação Artificial/veterinária
Masculino
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


  5 / 1223 MEDLINE  
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[PMID]:28237327
[Au] Autor:Anel-López L; Garcia-Álvarez O; Maroto-Morales A; Tarantini T; Del Olmo D; Ortiz JA; Martinez EA; Roca J; Vazquez JM; Garde JJ; Parrilla I
[Ad] Endereço:SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain.
[Ti] Título:Optimization of protocols for Iberian red deer (Cervus elaphus hispanicus) sperm handling before sex sorting by flow cytometry.
[So] Source:Theriogenology;92:129-136, 2017 Apr 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Currently, sperm reproductive biotechnologies such as sex sorting and cryopreservation are undoubtedly valuable tools for improving the economic and biological efficiency of red deer production systems. In this context, and because of the particular characteristics of this species (extensive exploitation typically far from laboratory facilities), a key goal is to optimize the design of an adequate handling protocol of sperm samples before samples are subjected to sex sorting and cryopreservation procedures to obtain better outputs from the application of these technologies. The main aim of this paper was to design an adequate protocol for Iberian red deer sperm handling before sex sorting by flow cytometry to obtain optimal yields when sex sorting is used in this species. Semen samples from 11 adult males were obtained by electroejaculation during the breeding season. In this study, we tested different protocols for the handling of Iberian red deer spermatozoa before sorting by using different concentrations of sperm (400 or 800 × 10 ) and adding or not Hoechst 33342 before the transport of samples to the sorting facilities. Based on the results, the most adequate method used to handle samples before sorting was transportation at a high sperm concentration (800 × 10 /mL) without Hoechst 33342. These transportation conditions in combination with Hoechst 33342 staining at 5.2 µL/mL once at the flow cytometry laboratory resulted in better (P < 0.05) sorting efficiency (99.9% of the samples showing split) than both, those samples transported at 400 × 10 sperm/mL (between 51.2 and 55.2% of the samples showing split) and those samples stained before transport at a sperm concentration of 400 × 10 sperm/mL (between 15.4 and 75.7% of the samples showing split). Sorting rates and sperm quality after sorting and cryopreservation was not affected (P > 0.05) by sperm handling before sorting. Moreover, the sorting yields were compatible with the practical application of these reproductive biotechnologies.
[Mh] Termos MeSH primário: Cervos/fisiologia
Citometria de Fluxo/veterinária
Pré-Seleção do Sexo/veterinária
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Animais
Criopreservação/veterinária
Masculino
Análise do Sêmen/veterinária
Preservação do Sêmen/veterinária
Pré-Seleção do Sexo/métodos
Manejo de Espécimes
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


  6 / 1223 MEDLINE  
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[PMID]:28078817
[Au] Autor:Wei YF; Chen FL; Tang SS; Mao AG; Li LG; Cheng LG; Chen C; Li FX; Wang B; Xu T; Zhang YJ; Li J; Wan JS
[Ad] Endereço:Kunming Police Dog Base of the Ministry of Public Security, Kunming, Yunnan, China.
[Ti] Título:Birth of puppies of predetermined sex after artificial insemination with a low number of sex-sorted, frozen-thawed spermatozoa in field conditions.
[So] Source:Anim Sci J;88(8):1232-1238, 2017 Aug.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate fertility and sex ratios after artificial insemination in dogs under field conditions. Semen was cryopreserved as unsorted (control) or was separated into X- and Y-chromosome-bearing sperm using a cell sorter. Sixty female dogs were inseminated with frozen-thawed spermatozoa of 100 × 10 unsorted (a dose in practice) and 4 × 10 sorted (X and Y group, respectively). A total of 20 dogs became pregnant and 126 puppies were born from the three groups. The percentage of parturition was similar for the X (5/20; 25.0%) and Y (4/20; 20.0%) group (P > 0.05), but lower than controls (11/20; 55.0%) (P < 0.05). Ultimately 28 out of the 32 puppies produced from X group were female (87.5%) and 19/22 (86.4%) puppies of Y group were male. In contrast, sex ratio (51.4% to 48.6%) in the control was significantly different from the X, Y group (P < 0.05). However, male and female puppies in the control had similar birth weights and weaning weights to those from the X and Y groups. This preliminary information indicated that normal puppies of predicted sex can be produced with low numbers of sorted cryopreserved dog spermatozoa at a farm level, making sperm-sexing technology potentially applicable for elite breeding units.
[Mh] Termos MeSH primário: Criopreservação
Fertilidade
Congelamento
Inseminação Artificial
Parto
Prenhez
Preservação do Sêmen
Pré-Seleção do Sexo/métodos
Razão de Masculinidade
Espermatozoides
[Mh] Termos MeSH secundário: Animais
Cães
Feminino
Masculino
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12763


  7 / 1223 MEDLINE  
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[PMID]:28043354
[Au] Autor:Anel-López L; García-Álvarez O; Parrilla I; Del Olmo D; Maroto-Morales A; Fernandez-Santos MR; Ortiz JA; Soler AJ; Martínez EM; Vazquez JM; Garde JJ
[Ad] Endereço:SaBio IREC (CSIC-UCLM-JCCM), Campus Universitario s. n., 02071 Albacete, Spain.
[Ti] Título:Effect of sex-sorting and cryopreservation on the post-thaw sperm quality of Iberian red deer spermatozoa.
[So] Source:Theriogenology;89:206-213, 2017 Feb.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen. Two straws per stag and sample type were analyzed immediately post-thaw and following a 2-h incubation period at 37 °C. Post-thaw total motility (TM) as assessed by CASA was not different (P < 0.05) among NS, BSS and YSS sperm. For XSS, post-thaw TM was lower (39%, P < 0.05) than that for NS (54%) or BSS (50%), but similar (P > 0.05) to that of YSS (47%) sperm. The percentage of apoptotic spermatozoa as assessed by PI/YO-PRO-1 and flow cytometry analysis, was higher (17%, P ≤ 0.05) for XSS sperm than NS (12%), BSS (13%) and YSS (14%) sperm. Following incubation there were no differences (P > 0.05) in TM or percent apoptosis among treatments. Post-thaw chromatin stability calculated as the DNA fragmentation index (%DFI) was similar among treatments; following incubation %DFI increased in all except YSS, which displayed the lowest value (P < 0.05). Artificial insemination of synchronized hinds yielded 44, 52 and 62% delivery rates for YSS, NS and standard frozen-thawed sperm, respectively (P < 0.05). Notably, 93 and 55% of fawns born were males for the YSS and NS spermatozoa, respectively (P < 0.05). In summary, Y-sorted sperm displayed acceptable post-thaw sperm evaluation parameters and the expected offspring sex ratio. More studies are needed to understand the source of sperm damage that may compromise the fertility of Y-sorted red deer sperm.
[Mh] Termos MeSH primário: Criopreservação/veterinária
Cervos
Pré-Seleção do Sexo/veterinária
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Animais
Criopreservação/métodos
Fragmentação do DNA
Citometria de Fluxo/veterinária
Inseminação Artificial/veterinária
Masculino
Preservação do Sêmen/métodos
Preservação do Sêmen/veterinária
Razão de Masculinidade
Motilidade Espermática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


  8 / 1223 MEDLINE  
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[PMID]:27678515
[Au] Autor:Holden SA; Fernandez-Fuertes B; Murphy C; Whelan H; O'Gorman A; Brennan L; Butler ST; Lonergan P; Fair S
[Ad] Endereço:Laboratory of Animal Reproduction, Department of Life Sciences, Faculty of Science and Engineering, University of Limerick, Limerick, Ireland.
[Ti] Título:Relationship between in vitro sperm functional assessments, seminal plasma composition, and field fertility after AI with either non-sorted or sex-sorted bull semen.
[So] Source:Theriogenology;87:221-228, 2017 Jan 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The hypothesis of this study was that different in vitro parameters are required to predict the in vivo fertility of non-sorted (NS) and sex-sorted (SS) semen. Thus, the aim was to correlate in vitro bull sperm functional parameters (experiment 1) and seminal plasma composition (experiment 2) with pregnancy rates using 2 cohorts of bulls (NS and SS). Experiment 1: ejaculates from each bull (n = 3 ejaculates per bull; n = 6 bulls for both NS and SS) were assessed for motility, thermal stress tolerance and morphology using microscopy, and viability, osmotic resistance, mitochondrial membrane potential, and acrosome integrity using flow cytometry. Fertilizing ability was assessed using IVF. Experiment 2: ejaculates (n = 3 per bull; n = 8 and 6 bulls for NS and SS, respectively) were collected, seminal plasma harvested and frozen and later analyzed for amino acid and fatty acid composition using gas chromatography mass spectrometry. In the NS cohort of bulls, there was no correlation between pregnancy rate and any of the sperm functional parameters assessed. However, within the SS cohort, motility and viability were correlated with pregnancy rate (r = 0.84 and 0.80, respectively; P < 0.05). There was no correlation between IVF outcome and pregnancy rate in either the SS or NS cohort of bulls. In the NS cohort of bulls, concentrations of the amino acid isoleucine and the fatty acid tricosylic acid (C23:0) were correlated with pregnancy rate (r = 0.80 and 0.74, respectively; P < 0.05). Within the SS cohort of bulls, the amino acid glutamic acid and the fatty acid arachidic acid (C20:0) were correlated with pregnancy rate (r = 0.84 and 0.82, respectively; P < 0.05). In conclusion, this study suggests that different in vitro markers of fertility are required to predict the fertility of NS and SS sperm.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Inseminação Artificial/veterinária
Sêmen/química
Pré-Seleção do Sexo/veterinária
[Mh] Termos MeSH secundário: Acrossomo
Animais
Sobrevivência Celular
Fragmentação do DNA
Feminino
Masculino
Potencial da Membrana Mitocondrial/fisiologia
Gravidez
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160929
[St] Status:MEDLINE


  9 / 1223 MEDLINE  
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[PMID]:27662774
[Au] Autor:Mikkola M; Taponen J
[Ad] Endereço:Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland; VikingGenetics Finland, Hollola, Finland. Electronic address: marja.mikkola@vikinggenetics.com.
[Ti] Título:Quality and developmental rate of embryos produced with sex-sorted and conventional semen from superovulated dairy cattle.
[So] Source:Theriogenology;87:135-140, 2017 Jan 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effect of sex-sorted semen compared with conventional semen on the outcome of embryo recovery, placing special emphasis on the quality, and developmental stage of embryos. Data were analyzed for 443 embryo collections with sex-sorted semen (SEX group) and 1528 with conventional semen (CONV group) in superovulated dairy heifers and cows. The insemination protocol for conventional semen included two inseminations, comprising a total dose of 30 million sperm passing into the uterine body. For sex-sorted semen, two (30%) to three (70%) deep uterine inseminations were performed, the total dose ranging from eight to 12 million sperm. The data were analyzed separately for heifers and cows. The total number of recovered structures was similar among the groups. The number of viable embryos decreased in the SEX groups compared with the CONV (with 1.4 and 3.2 fewer embryos in heifers and cows, correspondingly, P < 0.001), and correspondingly the proportions of unfertilized ova and degenerated embryos increased in the SEX groups (P < 0.001). The proportion of unsuccessful collections, yielding no transferable embryos, increased in the SEX groups for both heifers (from 7.2% to 11.2%, P = 0.025) and cows (from 9.0% to 20.7%, P < 0.001). Regarding the quality of viable embryos, the quality grades were superior in the CONV group compared with the SEX group for heifers (P < 0.001) and cows (P < 0.001). The proportion of grade 1 embryos decreased by 6.5 percentage points in heifers and 11.9 percentage points in cows when sex-sorted semen was used. Correspondingly, the proportions of grade 2 and 3 embryos increased in heifers and cows when sexed semen was used. The mean developmental stages of embryo collections were numerically slightly lower in the SEX group. In heifers, the delay in developmental stage was statistically significant (P = 0.001), but in cows, there was only a tendency toward that (P = 0.067). In conclusion, sex-sorted sperm decreased the transferable embryo yield and increased the risk of a recovery yielding no transferable embryos. Furthermore, use of sex-sorted semen decreased the proportion of grade 1 embryos. In addition, it also seemed to delay embryonic development, although the delay in embryonic development was minimal and its biological relevance remains undefined. Despite the compromised embryo production, taken into account the optimization of recipient resources, the use of sex-sorted semen is advantageous, especially in superovulated heifers, which are of most importance in the modern breeding strategies using genomic selection.
[Mh] Termos MeSH primário: Bovinos/embriologia
Embrião de Mamíferos/fisiologia
Desenvolvimento Embrionário/fisiologia
Sêmen
Pré-Seleção do Sexo
Superovulação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bovinos/fisiologia
Feminino
Inseminação Artificial/veterinária
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


  10 / 1223 MEDLINE  
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[PMID]:27060979
[Au] Autor:Gibb Z; Grupen CG; Maxwell WM; Morris LH
[Ad] Endereço:Faculty of Veterinary Science, The University of Sydney, New South Wales, Australia.
[Ti] Título:Field fertility of liquid stored and cryopreserved flow cytometrically sex-sorted stallion sperm.
[So] Source:Equine Vet J;49(2):160-166, 2017 Mar.
[Is] ISSN:2042-3306
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:REASONS FOR PERFORMING STUDY: The fertility of sex-sorted, cryopreserved stallion sperm must be improved for the sex-sorting technology to be applied commercially. OBJECTIVES: To optimise the conditions used to liquid store stallion sperm prior to sex-sorting and assess the fertility of sperm following sex-sorting and cryopreservation. STUDY DESIGN: Both in vitro experiment and randomised controlled trial in healthy, client-owned mares. METHODS: Stallion ejaculates (n = 9) were diluted in either a skimmed milk (KMT) or BSA (I-BSA) based media to 25 × 10 sperm/ml directly (+SP25) or washed to remove seminal plasma and diluted to 25 or 111 × 10 sperm/ml (-SP25 and -SP111). Sperm were stored for 18 h at 10 to 15°C and -SP25 and +SP25 treatments were centrifuged and resuspended to 111 × 10 sperm/ml. Sperm were incubated under H33342 staining conditions and motility, viability and acrosome integrity assessed. Semen was collected from stallions (n = 4), liquid stored at 10-15°C for up to 5 h and sperm either cryopreserved directly, sex-sorted and cryopreserved, or sex-sorted and returned to liquid storage until insemination. Low-dose hysteroscopic insemination was performed in 23 mares randomly allocated to the semen preparation group and pregnancy determined following embryo flushing on Day 9 after ovulation, or via transrectal ultrasonography on Day 14 after ovulation. RESULTS: Skimmed milk was superior to I-BSA in maintaining motility, viability and acrosome integrity. Seminal plasma removal did not affect the parameters measured at the concentrations examined. Conception rates did not differ significantly between the groups, although a high incidence of pregnancy loss was observed in both the cryopreserved groups. CONCLUSIONS: While the conception rates achieved are among the highest yet reported for sex-sorted, cryopreserved stallion sperm, the high incidence of pregnancy loss suggests that the development of the resulting embryos was significantly impaired by the sperm processing treatments.
[Mh] Termos MeSH primário: Criopreservação/veterinária
Cavalos/fisiologia
Preservação do Sêmen/veterinária
Pré-Seleção do Sexo/veterinária
[Mh] Termos MeSH secundário: Acrossomo/fisiologia
Animais
Sobrevivência Celular
Feminino
Inseminação Artificial/veterinária
Masculino
Gravidez
Motilidade Espermática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160411
[St] Status:MEDLINE
[do] DOI:10.1111/evj.12583



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