Base de dados : MEDLINE
Pesquisa : E05.393.640 [Categoria DeCS]
Referências encontradas : 10990 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1099 ir para página                         

  1 / 10990 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28465148
[Au] Autor:Minucci A; De Paolis E; Concolino P; De Bonis M; Rizza R; Canu G; Scaglione GL; Mignone F; Scambia G; Zuppi C; Capoluongo E
[Ad] Endereço:Laboratory of Clinical Molecular and Personalized Diagnostics, Institute of Biochemistry and Clinical Biochemistry, Teaching and Research Hospital "Agostino Gemelli" Foundation, Rome, Italy. Electronic address: angelo.minucci@virgilio.it.
[Ti] Título:Competitive PCR-High Resolution Melting Analysis (C-PCR-HRMA) for large genomic rearrangements (LGRs) detection: A new approach to assess quantitative status of BRCA1 gene in a reference laboratory.
[So] Source:Clin Chim Acta;470:83-92, 2017 Jul.
[Is] ISSN:1873-3492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM OF THE STUDY: Evaluation of copy number variation (CNV) in BRCA1/2 genes, due to large genomic rearrangements (LGRs), is a mandatory analysis in hereditary breast and ovarian cancers families, if no pathogenic variants are found by sequencing. LGRs cannot be detected by conventional methods and several alternative methods have been developed. Since these approaches are expensive and time consuming, identification of alternative screening methods for LGRs detection is needed in order to reduce and optimize the diagnostic procedure. The aim of this study was to investigate a Competitive PCR-High Resolution Melting Analysis (C-PCR-HRMA) as molecular tool to detect recurrent BRCA1 LGRs. MATERIAL AND METHODS: C-PCR-HRMA was performed on exons 3, 14, 18, 19, 20 and 21 of the BRCA1 gene; exons 4, 6 and 7 of the ALB gene were used as reference fragments. RESULTS: This study showed that it is possible to identify recurrent BRCA1 LGRs, by melting peak height ratio between target (BRCA1) and reference (ALB) fragments. Furthermore, we underline that a peculiar amplicon-melting profile is associated to a specific BRCA1 LGR. All C-PCR-HRMA results were confirmed by Multiplex ligation-dependent probe amplification. CONCLUSIONS: C-PCR-HRMA has proved to be an innovative, efficient and fast method for BRCA1 LGRs detection. Given the sensitivity, specificity and ease of use, c-PCR-HRMA can be considered an attractive and powerful alternative to other methods for BRCA1 CNVs screening, improving molecular strategies for BRCA testing in the context of Massive Parallel Sequencing.
[Mh] Termos MeSH primário: Rearranjo Gênico
Genes BRCA1
Genoma Humano/genética
Laboratórios
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Variações do Número de Cópias de DNA
Éxons/genética
Feminino
Seres Humanos
Desnaturação de Ácido Nucleico
Neoplasias Ovarianas/genética
Reação em Cadeia da Polimerase/normas
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  2 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29215948
[Au] Autor:Osathanunkul M; Dheeranupattana S; Rotarayanont S; Sookkhee S; Osathanunkul K; Madesis P
[Ad] Endereço:a Department of Biology, Faculty of Science , Chiang Mai University , Chiang Mai , Thailand.
[Ti] Título:Evaluation of suitable DNA regions for molecular identification of high value medicinal plants in genus Kaempferia.
[So] Source:Nucleosides Nucleotides Nucleic Acids;36(12):726-735, 2017 Dec 02.
[Is] ISSN:1532-2335
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA barcoding coupled high resolution melting (Bar-HRM) is an emerging method for species discrimination based on DNA dissociation kinetics. The aim of this work was to evaluate the suitability of different primer sets, derived from selected DNA regions, for Bar-HRM analysis of species in Kaempferia (Zingiberaceae). Four primer pairs were evaluated (rbcL, rpoC, trnL and ITS1). It was observed that the ITS1 barcode was the most useful DNA barcoding region overall for species discrimination out of all of the regions and primers assessed. Thus, the primer pair derived from the ITS1 region was the single most effective region for the identification of the tested species, whereas the rbcL primer pair gave the lowest resolution. Our Bar-HRM developed here would not only be useful for identification of Kaempferia plant specimens lacking essential parts for morphological identification but will be useful for authenticating products in powdered form of a high value medicinal species Kaempferia parviflora, in particular.
[Mh] Termos MeSH primário: Código de Barras de DNA Taxonômico/métodos
DNA de Plantas/química
DNA de Plantas/genética
Zingiberaceae/classificação
Zingiberaceae/genética
[Mh] Termos MeSH secundário: Primers do DNA/genética
Mineração de Dados
Desnaturação de Ácido Nucleico
Plantas Medicinais/classificação
Plantas Medicinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Plant)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1080/15257770.2017.1391393


  3 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29020084
[Au] Autor:Osathanunkul M; Ounjai S; Osathanunkul R; Madesis P
[Ad] Endereço:Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
[Ti] Título:Evaluation of a DNA-based method for spice/herb authentication, so you do not have to worry about what is in your curry, buon appetito!
[So] Source:PLoS One;12(10):e0186283, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It is long believed that some spices may help protect against certain chronic conditions. Spices are usually parts of plants that have been powdered into small pieces. Have you ever wondered what the curry powder in your dish is made of? The aim of this work was to develop an appropriate DNA-based method for assessment of spice identity. Selecting the best marker for species recognition in the Zingiberaceae family. Six DNA regions were investigated in silico, including ITS, matK, rbcL, rpoC, trnH-psbA and trnL. Then, only four regions (ITS, matK, rbcL and trnH-psbA) were included in the simulated HRM (High-resolution Melting) analysis as the results from previous analysis showed that rpoC and trnL may not be suitable to be used to identify Zingiberaceae species in HRM analysis based on both the percentage of nucleotide variation and GC content. Simulated HRM analysis was performed to test the feasibility of Bar-HRM. We found that ITS2 is the most effective region to be used for identification of the studied species and thus was used in laboratory HRM analysis. All seven tested Zingiberaceae plants were then able to be distinguished using the ITS2 primers in laboratory HRM. Most importantly the melting curves gained from fresh and dried tissue overlapped, which is a crucial outcome for the applicability of the analysis. The method could be used in an authentication test for dried products. In the authentication test, only one of seven store-sold Zingiberaceae products that were tested contained the species listed on their labels, while we found substitution/contamination of the tested purchased products in the rest.
[Mh] Termos MeSH primário: DNA de Plantas/análise
Plantas Medicinais/genética
Especiarias/análise
[Mh] Termos MeSH secundário: Sequência de Bases
Simulação por Computador
DNA Espaçador Ribossômico/genética
Marcadores Genéticos
Desnaturação de Ácido Nucleico/genética
Reação em Cadeia da Polimerase
Zingiberaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer); 0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186283


  4 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28966282
[Au] Autor:Ito Y; Matsuo M; Osawa T; Hari Y
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Tokushima Bunri University.
[Ti] Título:Triplex- and Duplex-Forming Abilities of Oligonucleotides Containing 2'-Deoxy-5-trifluoromethyluridine and 2'-Deoxy-5-trifluoromethylcytidine.
[So] Source:Chem Pharm Bull (Tokyo);65(10):982-988, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A facile synthesis of 2'-deoxy-5-trifluoromethyluridine and 2'-deoxy-5-trifluoromethylcytidine phosphoramidites from commercially available 2'-deoxyuridine and 2'-deoxycytidine was achieved, respectively. The obtained phosphoramidites were incorporated into oligonucleotides, and their binding affinity to double-stranded DNA (dsDNA) and single-stranded RNA (ssRNA) was evaluated by UV-melting experiments. The triplex-forming abilities of oligonucleotides including 5-trifluoromethylpyrimidine nucleobases with dsDNA were decreased. Especially, the stability of the triplex containing a trifluoromethylcytosine ( C)-GC base triplet was low, likely due to the low pK of protonated C by the electron-withdrawing trifluoromethyl group. A slight decrease in stability of the duplex formed with ssRNA by oligonucleotides including 5-trifluoromethylpyrimidine nucleobases was only observed, suggesting that they might be applicable to various ssRNA-targeted technologies using features of fluorine atoms.
[Mh] Termos MeSH primário: Desoxicitidina/análogos & derivados
Desoxiuridina/análogos & derivados
Oligonucleotídeos/síntese química
[Mh] Termos MeSH secundário: Pareamento de Bases
DNA/química
DNA/metabolismo
Cinética
Desnaturação de Ácido Nucleico/efeitos da radiação
RNA/química
RNA/metabolismo
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (triplex DNA); 0W860991D6 (Deoxycytidine); 63231-63-0 (RNA); 9007-49-2 (DNA); W78I7AY22C (Deoxyuridine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00530


  5 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28965786
[Au] Autor:Miranda P; Oliveira LM; Weber G
[Ad] Endereço:Departamento de Física, Universidade Federal de Minas Gerais, Belo Horizonte-MG 31270-901, Brazil.
[Ti] Título:Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes.
[So] Source:Biophys Chem;230:62-67, 2017 Nov.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cy3 and Cy5 dyes linked to the 5' end of a double stranded DNA molecule are known to attach to both strands in a way that is very similar to an additional base pair and has a stabilizing effect on the oligonucleotide. Here we adapt the Peyrard-Bishop mesoscopic model to incorporate cyanine dyes and use the technique of thermal equivalence to obtain the appropriate parameters from existing melting temperatures. We have found that the stacking parameters are in the same range of ordinary AT and CG base pairs, in particular Cy3-A was found to be most rigidly stacked. While the cyanines stabilize the AT hydrogen bonds quite strongly the CG bonds are mostly unaffected.
[Mh] Termos MeSH primário: Carbocianinas/química
DNA/química
[Mh] Termos MeSH secundário: Pareamento de Bases
Carbocianinas/metabolismo
DNA/metabolismo
Ligações de Hidrogênio
Modelos Moleculares
Desnaturação de Ácido Nucleico
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (cyanine dye 3); 0 (cyanine dye 5); 9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


  6 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28941814
[Au] Autor:Basher MA; Rahman KM; Jackson PJM; Thurston DE; Fox KR
[Ad] Endereço:Biological Sciences, Life Sciences Building 85, University of Southampton, Southampton SO17 1BJ, UK.
[Ti] Título:Sequence-selective binding of C8-conjugated pyrrolobenzodiazepines (PBDs) to DNA.
[So] Source:Biophys Chem;230:53-61, 2017 Nov.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:DNA footprinting and melting experiments have been used to examine the sequence-specific binding of C8-conjugates of pyrrolobenzodiazepines (PBDs) and benzofused rings including benzothiophene and benzofuran, which are attached using pyrrole- or imidazole-containing linkers. The conjugates modulate the covalent attachment points of the PBDs, so that they bind best to guanines flanked by A/T-rich sequences on either the 5'- or 3'-side. The linker affects the binding, and pyrrole produces larger changes than imidazole. Melting studies with 14-mer oligonucleotide duplexes confirm covalent attachment of the conjugates, which show a different selectivity to anthramycin and reveal that more than one ligand molecule can bind to each duplex.
[Mh] Termos MeSH primário: Benzodiazepinas/química
DNA/química
Pirróis/química
[Mh] Termos MeSH secundário: Antramicina/química
Antramicina/metabolismo
Sequência de Bases
Benzodiazepinas/metabolismo
Sítios de Ligação
DNA/metabolismo
Pegada de DNA
Desoxirribonuclease I/metabolismo
Guanina/química
Simulação de Dinâmica Molecular
Conformação de Ácido Nucleico
Desnaturação de Ácido Nucleico
Oligonucleotídeos/química
Oligonucleotídeos/metabolismo
Pirróis/metabolismo
Espectrometria de Fluorescência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotides); 0 (Pyrroles); 0 (pyrrolo(2,1-c)(1,4)benzodiazepine); 0WZD9Y66WN (Anthramycin); 12794-10-4 (Benzodiazepines); 5Z93L87A1R (Guanine); 9007-49-2 (DNA); EC 3.1.21.1 (Deoxyribonuclease I)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  7 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28887044
[Au] Autor:Liu Y; Berrido AM; Hua ZC; Tse-Dinh YC; Leng F
[Ad] Endereço:Biomolecular Sciences Institute, Florida International University, Miami, FL 33199, United States; Department of Chemistry & Biochemistry, Florida International University, Miami, FL 33199, United States; School of Life Sciences, Nanjing University, Nanjing 210023, Jiangsu Province, PR China.
[Ti] Título:Biochemical and biophysical properties of positively supercoiled DNA.
[So] Source:Biophys Chem;230:68-73, 2017 Nov.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this paper we successfully developed a procedure to generate the (+) supercoiled (sc) plasmid DNA template pZXX6 in the milligram range. With the availability of the (+) sc DNA, we are able to characterize and compare certain biochemical and biophysical properties of (+) sc, (-) sc, and relaxed (rx) DNA molecules using different techniques, such as UV melting, circular dichroism, and fluorescence spectrometry. Our results show that (+) sc, (-) sc, and rx DNA templates can only be partially melted due to the fact that these DNA templates are closed circular DNA molecules and the two DNA strands cannot be completely separated upon denaturation at high temperatures. We also find that the fluorescence intensity of a DNA-binding dye SYTO12 upon binding to the (-) sc DNA is significantly higher than that of its binding to the (+) sc DNA. This unique property may be used to differentiate the (-) sc DNA from the (+) sc DNA. Additionally, we demonstrate that E. coli topoisomerase I cannot relax the (+) sc DNA. In contrast, E. coli DNA gyrase can efficiently convert the (+) sc DNA to the (-) sc DNA. Furthermore, our dialysis competition assays show that DNA intercalators prefer binding to the (-) sc DNA.
[Mh] Termos MeSH primário: DNA Super-Helicoidal/química
[Mh] Termos MeSH secundário: Dicroísmo Circular
DNA Girase/metabolismo
DNA Topoisomerases Tipo I/metabolismo
DNA Super-Helicoidal/metabolismo
Eletroforese em Gel de Ágar
Escherichia coli/metabolismo
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Substâncias Intercalantes/química
Substâncias Intercalantes/metabolismo
Desnaturação de Ácido Nucleico/efeitos da radiação
Plasmídeos/genética
Plasmídeos/metabolismo
Espectrometria de Fluorescência
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Superhelical); 0 (Fluorescent Dyes); 0 (Intercalating Agents); EC 5.99.1.2 (DNA Topoisomerases, Type I); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE


  8 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28825481
[Au] Autor:Mary V; Haris P; Varghese MK; Aparna P; Sudarsanakumar C
[Ad] Endereço:School of Pure and Applied Physics, Mahatma Gandhi University , Kottayam, Kerala 686560, India.
[Ti] Título:Experimental Probing and Molecular Dynamics Simulation of the Molecular Recognition of DNA Duplexes by the Flavonoid Luteolin.
[So] Source:J Chem Inf Model;57(9):2237-2249, 2017 Sep 25.
[Is] ISSN:1549-960X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Luteolin (C H O ) is an important flavonoid found in many fruits, plants, medicinal herbs, and vegetables exhibiting many pharmacological properties. The anticancer, antitumor, antioxidant, and anti-inflammatory activities of luteolin have been reported. The pharmacological action of small molecules is dependent upon its interaction with biomacromolecules. The interactions of small molecules with DNA play a major role in the transcription and translation process. In this work, we explored the energetic profile of DNA-luteolin interaction by isothermal titration calorimetry (ITC). The effect of temperature and salt concentration on DNA binding was examined by UV-Vis method. The mode of interaction was further probed by UV melting temperature analysis and differential scanning calorimetry. An atomic level insight on the recognition of luteolin with DNA was achieved by employing molecular dynamics (MD) simulation on luteolin in complex with AT- and GC-rich DNA sequences. AMBER force field proves to be appropriate in providing an understanding on the binding mode and specificity of luteolin with duplex DNA. MD results suggest a minor groove binding of luteolin with DNA and the binding free energy obtained is in agreement with the experimental results.
[Mh] Termos MeSH primário: DNA/metabolismo
Luteolina/metabolismo
Simulação de Dinâmica Molecular
[Mh] Termos MeSH secundário: Animais
Bovinos
DNA/química
Ligações de Hidrogênio
Conformação de Ácido Nucleico
Desnaturação de Ácido Nucleico
Termodinâmica
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA); KUX1ZNC9J2 (Luteolin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jcim.6b00747


  9 / 10990 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28818830
[Au] Autor:Pryor RJ; Myrick JT; Palais RA; Sundberg SO; Paek JY; Wittwer CT; Knight IT
[Ad] Endereço:Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT.
[Ti] Título:High-Speed Melting Analysis: The Effect of Melting Rate on Small Amplicon Microfluidic Genotyping.
[So] Source:Clin Chem;63(10):1624-1632, 2017 Oct.
[Is] ISSN:1530-8561
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS: Using a microfluidic platform for serial PCR and melting analysis, 4 targets containing single nucleotide variants were amplified and then melted at different rates over a 250-fold range from 0.13 to 32 °C/s. Genotypes (n = 1728) were determined manually by visual inspection after background removal, normalization, and conversion to negative derivative plots. Differences between genotypes were quantified by a genotype discrimination ratio on the basis of inter- and intragenotype differences using the absolute value of the maximum vertical difference between curves as a metric. RESULTS: Different homozygous curves were genotyped by melting temperature and heterozygous curves were identified by shape. Technical artifacts preventing analysis (0.3%), incorrect (0.06%), and indeterminate (0.4%) results were minimal, occurring mostly at slow melting rates (0.13-0.5 °C/s). Genotype discrimination was maximal at around 8 °C/s (2-8 °C/s for homozygotes and 8-16 °C/s for heterozygotes), and no genotyping errors were made at rates >0.5 °C/s. PCR was completed in 10-12.2 min, followed by melting curve acquisition in 4 min down to <1 s. CONCLUSIONS: Microfluidics enables genotyping by melting analysis at rates up to 32 °C/s, requiring <1 s to acquire an entire melting curve. High-speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons. Combined with extreme PCR, high-speed melting promises nucleic acid amplification and genotyping in < 1 min.
[Mh] Termos MeSH primário: DNA/genética
Técnicas de Genotipagem/métodos
Técnicas Analíticas Microfluídicas/métodos
Desnaturação de Ácido Nucleico
Reação em Cadeia da Polimerase/métodos
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Desenho de Equipamento
Genótipo
Técnicas de Genotipagem/economia
Técnicas de Genotipagem/instrumentação
Heterozigoto
Homozigoto
Seres Humanos
Técnicas Analíticas Microfluídicas/economia
Técnicas Analíticas Microfluídicas/instrumentação
Reação em Cadeia da Polimerase/economia
Reação em Cadeia da Polimerase/instrumentação
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1373/clinchem.2017.276147


  10 / 10990 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28793208
[Au] Autor:Carr CE; Marky LA
[Ad] Endereço:Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska.
[Ti] Título:Investigation of the Melting Behavior of DNA Three-Way Junctions in the Closed and Open States.
[So] Source:Biophys J;113(3):529-539, 2017 Aug 08.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intramolecular three-way junctions are commonly found in both DNA and RNA. These structures are functionally relevant in ribozymes, riboswitches, rRNA, and during replication. In this work, we present a thermodynamic description of the unfolding of DNA intramolecular three-way junctions. We used a combination of spectroscopic and calorimetric techniques to investigate the folding/unfolding thermodynamics of two three-way junctions with a closed (Closed-J) or open (Open-J) junction and their appropriate control stem-loop motifs (GAAATT-Hp, CTATC-Hp, and Dumbbell). The overall results show that both junctions are stable over a wide range of salt concentrations. However, Open-J is more stable due to a higher enthalpy contribution from the formation of a higher number of basepair stacks whereas Closed-J has a defined structure and retains the basepair stacking of all three stems. The comparison of the experimental results of Closed-J and Open-J with those of their component stem-loop motifs allowed us to be more specific about their cooperative unfolding. For instance, Closed-J sacrifices thermal stability of the Dumbbell structure to maintain an overall folded state. At higher salt concentration, the simultaneous unfolding of the above domains is lost, resulting in the unfolding of the three separate stems. In contrast, the junction of Open-J in low salt retains the thermal and enthalpic stability of the Dumbbell structure although sacrificing stability of the CTATC stem. The relative stability of Dumbbell is the primary reason for the higher ΔG° , or free energy, value seen for Open-J at low salt. Higher salt not only maintains thermal stability of the Dumbbell structure in Open-J but causes the CTATC stem to fully fold.
[Mh] Termos MeSH primário: DNA/química
[Mh] Termos MeSH secundário: Pareamento de Bases
Sequência de Bases
DNA/genética
Desnaturação de Ácido Nucleico
Temperatura de Transição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE



página 1 de 1099 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde