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Pesquisa : E05.393.760.700.825 [Categoria DeCS]
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  1 / 15 MEDLINE  
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[PMID]:29458686
[Au] Autor:Anson LW; Chau K; Sanderson N; Hoosdally S; Bradley P; Iqbal Z; Phan H; Foster D; Oakley S; Morgan M; Peto TEA; Modernizing Medical Microbiology Informatics Group Mmmig; Crook DW; Pankhurst LJ
[Ad] Endereço:1​Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, UK.
[Ti] Título:DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing.
[So] Source:J Med Microbiol;67(3):347-357, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Speed of bloodstream infection diagnosis is vital to reduce morbidity and mortality. Whole genome sequencing (WGS) performed directly from liquid blood culture could provide single-assay species and antibiotic susceptibility prediction; however, high inhibitor and human cell/DNA concentrations limit pathogen recovery. We develop a method for the preparation of bacterial DNA for WGS-based diagnostics direct from liquid blood culture. METHODOLOGY: We evaluate three commercial DNA extraction kits: BiOstic Bacteraemia, Amplex Hyplex and MolYsis Plus. Differential centrifugation, filtration, selective lysis and solid-phase reversible immobilization bead clean-up are tested to improve human cells/DNA and inhibitor removal. Using WGS (Illumina/MinION), we assess human DNA removal, pathogen recovery, and predict species and antibiotic susceptibility inpositive blood cultures of 44 Gram-negative and 54 Staphylococcus species.Results/Key findings. BiOstic kit extractions yield the greatest mean DNA concentration, 94-301 ng µl , versus 0-2.5 ng µl using Amplex and MolYsis kits. However, we note higher levels of inhibition (260/280 ratio 0.9-2.1) and human DNA (0.0-4.4×10 copies) in BiOstic extracts. Differential centrifugation (2000 g, 1 min) prior to BiOstic extraction reduces human DNA by 63-89 % with selective lysis minimizing by a further 62 %. Post-extraction bead clean-up lowers inhibition. Overall, 67 % of sequenced samples (Illumina MiSeq) contain <10 % human DNA, with >93 % concordance between WGS-based species and susceptibility predictions and clinical diagnosis. If >60 % of sequencing reads are human (7/98 samples) susceptibility prediction becomes compromised. Novel MinION-based WGS (n=9) currently gives rapid species identification but not susceptibility prediction. CONCLUSION: Our method for DNA preparation allows WGS-based diagnosis direct from blood culture bottles, providing species and antibiotic susceptibility prediction in a single assay.
[Mh] Termos MeSH primário: Bacteriemia/diagnóstico
Hemocultura
DNA Bacteriano/isolamento & purificação
Genoma Bacteriano
Sequenciamento Completo do Genoma
[Mh] Termos MeSH secundário: Bacteriemia/microbiologia
Infecções Relacionadas a Cateter/diagnóstico
Infecções Relacionadas a Cateter/microbiologia
DNA Bacteriano/análise
DNA Bacteriano/genética
Escherichia coli/genética
Seres Humanos
Testes de Sensibilidade Microbiana
Técnicas de Diagnóstico Molecular/métodos
Kit de Reagentes para Diagnóstico
Análise de Sequência de DNA/métodos
Staphylococcus aureus/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000664


  2 / 15 MEDLINE  
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[PMID]:29254926
[Au] Autor:Hu PF; Xu JP; Ai C; Shao XJ; Wang HL; Dong YM; Cui XZ; Fuhe Y; Xiumei X
[Ad] Endereço:State Key Laboratory for Molecular Biology of Special Economic Animals, Key Laboratory of Special Economic Animal Genetics and Breeding, Ministry of Agriculture, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
[Ti] Título:Screening weight related genes of velvet antlers by whole genome re-sequencing.
[So] Source:Yi Chuan;39(11):1090-1101, 2017 Nov 20.
[Is] ISSN:0253-9772
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The velvet antler is a special organ that has important biological significance for deer, and its growth is a complicated biological metabolism process. Growing evidence suggests that genetics factors play essential roles in the weight of velvet antlers. In this study, we investigated five sika deer (Cervus nippon) populations under the same feeding condition, and screened genetic variations in the 100 samples (including 50 heavy and 50 light velvet antler weight samples) by whole genome re-sequencing. The results showed that 94 genetic variations were related to the velvet antler weight, among which two single nucleotide polymorphism (SNP) sites were located on the exon regions of OAS2 and ALYREF/THOC4, respectively. Furthermore, ALYREF/THOC4 is highly expressed in the velvet antler. The biological functions of these genetic variations were highly related to the growth and development of deer velvet antlers. Collectively, we screened genes related to the velvet antler weight in sika deer populations by whole genome re-sequencing and identified 94 sites as candidate genetic variations related to the velvet antler weight. We hope that it will contribute to further mechanistic studies of velvet antler development and weight variations.
[Mh] Termos MeSH primário: Chifres de Veado
Cervos/genética
Tamanho do Órgão/genética
Sequenciamento Completo do Genoma
[Mh] Termos MeSH secundário: Animais
Chifres de Veado/crescimento & desenvolvimento
Variação Genética
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.16288/j.yczz.17-116


  3 / 15 MEDLINE  
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[PMID]:28749333
[Au] Autor:Bassi C; Taha MK; Merle C; Hong E; Lévy-Bruhl D; Barret AS; Mounchetrou Njoya I
[Ad] Endereço:Santé publique France, French National Public Health Agency, Regional Unit (Cire) Ile-de-France, Paris, France.
[Ti] Título:A cluster of invasive meningococcal disease (IMD) caused by Neisseria meningitidis serogroup W among university students, France, February to May 2017.
[So] Source:Euro Surveill;22(28), 2017 Jul 13.
[Is] ISSN:1560-7917
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:Between February and May 2017, two cases of invasive meningococcal disease caused by a new, rapidly expanding serogroup W meningococci variant were reported among students of an international university in Paris. Bacteriological investigations showed that isolates shared identical genotypic formula (W:P1.5,2:F1-1:cc11) and belonged to the South American/UK lineage. A vaccination campaign was organised that aimed at preventing new cases linked to potential persistence of the circulation of the bacteria in the students.
[Mh] Termos MeSH primário: Infecções Meningocócicas/diagnóstico
Neisseria meningitidis Sorogrupo W-135/isolamento & purificação
[Mh] Termos MeSH secundário: Busca de Comunicante
Febre/etiologia
Genótipo
Seres Humanos
Masculino
Infecções Meningocócicas/sangue
Infecções Meningocócicas/microbiologia
Tipagem Molecular
Neisseria meningitidis Sorogrupo W-135/genética
Paris
Sorogrupo
Estudantes
Sequenciamento Completo do Genoma
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  4 / 15 MEDLINE  
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[PMID]:29302025
[Au] Autor:Guéant JL; Chéry C; Oussalah A; Nadaf J; Coelho D; Josse T; Flayac J; Robert A; Koscinski I; Gastin I; Filhine-Tresarrieu P; Pupavac M; Brebner A; Watkins D; Pastinen T; Montpetit A; Hariri F; Tregouët D; Raby BA; Chung WK; Morange PE; Froese DS; Baumgartner MR; Benoist JF; Ficicioglu C; Marchand V; Motorin Y; Bonnemains C; Feillet F; Majewski J; Rosenblatt DS
[Ad] Endereço:INSERM, UMR_S954 Nutrition-Genetics-Environmental Risk Exposure and Reference Centre of Inborn Metabolism Diseases, University of Lorraine and University Hospital Centre of Nancy (CHRU Nancy), 54505, Nancy, France. jean-louis.gueant@univ-lorraine.fr.
[Ti] Título:APRDX1 mutant allele causes a MMACHC secondary epimutation in cblC patients.
[So] Source:Nat Commun;9(1):67, 2018 01 04.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To date, epimutations reported in man have been somatic and erased in germlines. Here, we identify a cause of the autosomal recessive cblC class of inborn errors of vitamin B metabolism that we name "epi-cblC". The subjects are compound heterozygotes for a genetic mutation and for a promoter epimutation, detected in blood, fibroblasts, and sperm, at the MMACHC locus; 5-azacytidine restores the expression of MMACHC in fibroblasts. MMACHC is flanked by CCDC163P and PRDX1, which are in the opposite orientation. The epimutation is present in three generations and results from PRDX1 mutations that force antisense transcription of MMACHC thereby possibly generating a H3K36me3 mark. The silencing of PRDX1 transcription leads to partial hypomethylation of the epiallele and restores the expression of MMACHC. This example of epi-cblC demonstrates the need to search for compound epigenetic-genetic heterozygosity in patients with typical disease manifestation and genetic heterozygosity in disease-causing genes located in other gene trios.
[Mh] Termos MeSH primário: Proteínas de Transporte/genética
Epistasia Genética
Erros Inatos do Metabolismo/genética
Mutação
Peroxirredoxinas/genética
Vitamina B 12/metabolismo
[Mh] Termos MeSH secundário: Alelos
Azacitidina/farmacologia
Sequência de Bases
Inibidores Enzimáticos/farmacologia
Saúde da Família
Feminino
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Heterozigoto
Seres Humanos
Masculino
Erros Inatos do Metabolismo/metabolismo
Linhagem
Sequenciamento Completo do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Enzyme Inhibitors); 0 (MMACHC protein, human); EC 1.11.1.15 (PRDX1 protein, human); EC 1.11.1.15 (Peroxiredoxins); M801H13NRU (Azacitidine); P6YC3EG204 (Vitamin B 12)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02306-5


  5 / 15 MEDLINE  
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[PMID]:29352281
[Au] Autor:Al-Harrasi I; Al-Yahyai R; Yaish MW
[Ad] Endereço:Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.
[Ti] Título:Differential DNA methylation and transcription profiles in date palm roots exposed to salinity.
[So] Source:PLoS One;13(1):e0191492, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a salt-adaptive plant, the date palm (Phoenix dactylifera L.) requires a suitable mechanism to adapt to the stress of saline soils. There is growing evidence that DNA methylation plays an important role in regulating gene expression in response to abiotic stresses, including salinity. Thus, the present study sought to examine the differential methylation status that occurs in the date palm genome when plants are exposed to salinity, and to identify salinity responsive genes that are regulated by DNA methylation. To achieve these, whole-genome bisulfite sequencing (WGBS) was employed and mRNA was sequenced from salinity-treated and untreated roots. The WGBS analysis included 324,987,795 and 317,056,091 total reads of the control and the salinity-treated samples, respectively. The analysis covered about 81% of the total genomic DNA with about 40% of mapping efficiency of the sequenced reads and an average read depth of 17-fold coverage per DNA strand, and with a bisulfite conversion rate of around 99%. The level of methylation within the differentially methylated regions (DMRs) was significantly (p < 0.05, FDR ≤ 0.05) increased in response to salinity specifically at the mCHG and mCHH sequence contexts. Consistently, the mass spectrometry and the enzyme-linked immunosorbent assay (ELISA) showed that there was a significant (p < 0.05) increase in the global DNA methylation in response to salinity. mRNA sequencing revealed the presence of 6,405 differentially regulated genes with a significant value (p < 0.001, FDR ≤ 0.05) in response to salinity. Integration of high-resolution methylome and transcriptome analyses revealed a negative correlation between mCG methylation located within the promoters and the gene expression, while a positive correlation was noticed between mCHG/mCHH methylation rations and gene expression specifically when plants grew under control conditions. Therefore, the methylome and transcriptome relationships vary based on the methylated sequence context, the methylated region within the gene, the protein-coding ability of the gene, and the salinity treatment. These results provide insights into interplay among DNA methylation and gene expression, and highlight the effect of salinity on the nature of this relationship, which may involve other genetic and epigenetic players under salt stress conditions. The results obtained from this project provide the first draft map of the differential methylome and transcriptome of date palm when exposed to an abiotic stress.
[Mh] Termos MeSH primário: Metilação de DNA
Phoeniceae/genética
Phoeniceae/metabolismo
Salinidade
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
DNA de Plantas/genética
DNA de Plantas/metabolismo
Epigênese Genética
Perfilação da Expressão Gênica
Genes de Plantas
Anotação de Sequência Molecular
Phoeniceae/crescimento & desenvolvimento
Fotossíntese
Raízes de Plantas/genética
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Regiões Promotoras Genéticas
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA de Plantas/genética
RNA de Plantas/metabolismo
Sequenciamento Completo do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Plant); 0 (RNA, Messenger); 0 (RNA, Plant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191492


  6 / 15 MEDLINE  
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[PMID]:28463423
[Au] Autor:Guo H; Benndorf R; Leichnitz D; Klassen JL; Vollmers J; Görls H; Steinacker M; Weigel C; Dahse HM; Kaster AK; de Beer ZW; Poulsen M; Beemelmanns C
[Ad] Endereço:Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Beutenbergstraße 11a, 07745, Jena, Germany.
[Ti] Título:Isolation, Biosynthesis and Chemical Modifications of Rubterolones A-F: Rare Tropolone Alkaloids from Actinomadura sp. 5-2.
[So] Source:Chemistry;23(39):9338-9345, 2017 Jul 12.
[Is] ISSN:1521-3765
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The discovery of six new, highly substituted tropolone alkaloids, rubterolones A-F, from Actinomadura sp. 5-2, isolated from the gut of the fungus-growing termite Macrotermes natalensis is reported. Rubterolones were identified by using fungus-bacteria challenge assays and a HRMS-based dereplication strategy, and characterised by NMR and HRMS analyses and by X-ray crystallography. Feeding experiments and subsequent chemical derivatisation led to a first library of rubterolone derivatives (A-L). Genome sequencing and comparative analyses revealed their putative biosynthetic pathway, which was supported by feeding experiments. This study highlights how gut microbes can present a prolific source of secondary metabolites.
[Mh] Termos MeSH primário: Actinomycetales/química
Alcaloides/biossíntese
Tropolona/química
[Mh] Termos MeSH secundário: Actinomycetales/classificação
Actinomycetales/genética
Alcaloides/química
Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Animais
Vias Biossintéticas/genética
Cromatografia Líquida de Alta Pressão
Cristalografia por Raios X
Intestinos/microbiologia
Isópteros/microbiologia
Espectroscopia de Ressonância Magnética
Espectrometria de Massas
Conformação Molecular
Família Multigênica
Filogenia
Sequenciamento Completo do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 7L6DL16P1T (Tropolone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/chem.201701005


  7 / 15 MEDLINE  
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[PMID]:29218572
[Au] Autor:Peng X; Pan H; Muhammad A; An H; Fang S; Li W; Zhang S
[Ad] Endereço:Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
[Ti] Título:Complete genome sequence of a new strain of Lagenaria siceraria endornavirus from China.
[So] Source:Arch Virol;163(3):805-808, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:An RNA virus tentatively named Lagenaria siceraria endornavirus-Hubei (LsEV-HuB) was isolated from Lagenaria siceraria var. hispida in Hubei, China. The LsEV-HuB genome consists of 15,098 bp and contains a single open reading frame (ORF) encoding a large protein with several conserved domains, including one helicase domain, one glycosyltransferase domain, two capsular polysaccharide synthesis protein (CPS) domains, and one RNA-dependent RNA polymerase (RdRp) domain. LsEV-HuB has nucleotide and amino acid sequence identities of 72.96% and 77.95%, respectively, to Lagenaria siceraria endornavirus-California (LsEV-CA), the closest relative of LsEV-HuB.
[Mh] Termos MeSH primário: Cucurbitaceae/virologia
DNA Viral/genética
Genoma Viral
Filogenia
Poliproteínas/genética
Vírus de RNA/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
China
Tamanho do Genoma
Fases de Leitura Aberta
Doenças das Plantas/virologia
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
Recombinação Genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Sequenciamento Completo do Genoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Polyproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3664-y


  8 / 15 MEDLINE  
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[PMID]:29247978
[Au] Autor:Xiao X; Garbutt CC; Hornicek F; Guo Z; Duan Z
[Ad] Endereço:Department of Orthopedics, Xijing Hospital, Forth Military Medical University, Xian 710032, Shaanxi, PR China; Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, 615 Charles E. Young. Dr. South, Los Angeles, CA 90095-6902, USA.
[Ti] Título:Advances in chromosomal translocations and fusion genes in sarcomas and potential therapeutic applications.
[So] Source:Cancer Treat Rev;63:61-70, 2018 Feb.
[Is] ISSN:1532-1967
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chromosomal translocations and fusion genes are very common in human cancer especially in subtypes of sarcomas, such as rhabdomyosarcoma, Ewing's sarcoma, synovial sarcoma and liposarcoma. The discovery of novel chromosomal translocations and fusion genes in different tumors are due to the advancement of next-generation sequencing (NGS) technologies such as whole genome sequencing. Recently, many novel chromosomal translocations and gene fusions have been identified in different types of sarcoma through NGS approaches. In addition to previously known sarcoma fusion genes, these novel specific fusion genes and associated molecular events represent important targets for novel therapeutic approaches in the treatment of sarcomas. This review focuses on recent advances in chromosomal translocations and fusion genes in sarcomas and their potential therapeutic applications in the treatment of sarcomas.
[Mh] Termos MeSH primário: Fusão Gênica/genética
Sarcoma/genética
Translocação Genética/genética
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Sequenciamento Completo do Genoma/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


  9 / 15 MEDLINE  
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[PMID]:29369557
[Au] Autor:Belova IV; Tochilina AG; Solovyeva IV; Efimov EI; Gorlova IS; Ivanova TP; Zhirnov VA
[Ti] Título:[Lactobacillus fermentum 90 TC-4 taxonomic status confirmation using whole genome sequencing and MALDI TOF mass spectrum].
[So] Source:Genetika;52(9):1021-8, 2016 Sep.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:With the use of whole genome sequencing, the taxonomic status of Lactobacillus fermentum 90 TC-4 strain from Russian collections were studied. Complex analysis of phenotypical and genetic properties was conducted using phenotypic and molecular genetic methods. The main characteristics of the genome and biochemical activity profile of the strain were determined. A comparative analysis of the mass spectrum of ribosomal proteins of the strain, its biochemical properties, a fragment of 16S rRNA gene sequencing, and the entire genome revealed that the present strain belongs to the species L. fermentum, confirming its taxonomic status in accordance with modern taxonomy.
[Mh] Termos MeSH primário: Lactobacillus fermentum
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Sequenciamento Completo do Genoma
[Mh] Termos MeSH secundário: Lactobacillus fermentum/classificação
Lactobacillus fermentum/genética
Lactobacillus fermentum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  10 / 15 MEDLINE  
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[PMID]:29368834
[Au] Autor:Vatlin AA; Bekker OB; Lysenkova LN; Korolev AM; Shchekotikhin AE; Danilenko VN
[Ti] Título:[Sequencing and analysis of the resistome of Streptomyces fradiae ATCC19609 in order to develop a test system for screening of new antimicrobial agents].
[So] Source:Genetika;52(6):723-7, 2016 Jun.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The paper provides the annotation and data on sequencing the antibiotic resistance genes in Streptomyces fradiae strain ATCC19609, highly sensitive to different antibiotics. Genome analysis revealed four groups of genes that determined the resistome of the tested strain. These included classical antibiotic resistance genes (nine aminoglycoside phosphotransferase genes, two beta-lactamase genes, and the genes of puromycin N-acetyltransferase, phosphinothricin N-acetyltransferase, and aminoglycoside acetyltransferase); the genes of ATP-dependent ABC transporters, involved in the efflux of antibiotics from the cell (MacB-2, BcrA, two-subunit MDR1); the genes of positive and negative regulation of transcription (whiB and padR families); and the genes of post-translational modification (serine-threonine protein kinases). A comparative characteristic of aminoglycoside phosphotransferase genes in S. fradiae ATCC19609, S. lividans TK24, and S. albus J1074, the causative agent of actinomycosis, is provided. The possibility of using the S. fradiae strain ATCC19609 as the test system for selection of the macrolide antibiotic oligomycin A derivatives with different levels of activity is demonstrated. Analysis of more than 20 semisynthetic oligomycin A derivatives made it possible to divide them into three groups according to the level of activity: inactive (>1 nmol/disk), 10 substances; with medium activity level (0.05­1 nmol/disk), 12 substances; and more active (0.01­0.05 nmol/disk), 2 substances. Important for the activity of semisynthetic derivatives is the change in the position of the 33rd carbon atom in the oligomycin A molecule.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Farmacorresistência Bacteriana
Genoma Bacteriano
Oligomicinas/farmacologia
Streptomyces
Sequenciamento Completo do Genoma
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Farmacorresistência Bacteriana/efeitos dos fármacos
Farmacorresistência Bacteriana/genética
Testes de Sensibilidade Microbiana
Streptomyces/genética
Streptomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Oligomycins); 05HQS4AI99 (oligomycin A)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE



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