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[PMID]:29381759
[Au] Autor:Narayan E; Sawyer G; Parisella S
[Ad] Endereço:School of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, New South Wales, Australia.
[Ti] Título:Faecal glucocorticoid metabolites and body temperature in Australian merino ewes (Ovis aries) during summer artificial insemination (AI) program.
[So] Source:PLoS One;13(1):e0191961, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reproductive wastage is a key issue for sheep producers, both regionally and globally. The reproductive output of farm animals can be influenced by physiological and environmental factors. Rapid and reliable quantification of physiological stress can provide a useful tool for designing and testing on-farm management interventions to improve farm animal welfare and productivity. In this study, we quantified physiological stress non-invasively using faecal glucocorticoid metabolites-FGMs analysis and body temperature measurements of 15 superovulated donor merino ewes (Ovis aries) during participation in artificial insemination (AI) program conducted during 2015/2016 Australian summer. We hypothesized that low percentage transferable embryos in donor merino ewes will be associated positively with higher body temperature and/or higher FGMs in these ewes. Temperature humidity index (THI) was calculated and found within high thermal stress range during the two AI trials. Overall, results showed none of the factors (ewe ID, AI trial no., THI or FGMs) were significant for reduced percentage transferrable embryos, except ewe body temperature was highly significant (p = 0.014). Within AI trial comparisons showed significant positive associations between higher FGMs and body temperature with reduced transferrable embryos. These results suggest that Australian merino ewes participating in summer AI trials can experience physiological stress. Prolonged activation of the stress endocrine response and high body temperature (e.g. ensued from heat stress) could impact on ewe reproductive output. Therefore, future research should apply minimally invasive physiological tools to gather baseline information on physiological stress in merino sheep to enable the development of new farm-friendly methods of managing stress.
[Mh] Termos MeSH primário: Fezes
Glucocorticoides/metabolismo
Inseminação Artificial
[Mh] Termos MeSH secundário: Animais
Austrália
Clima
Feminino
Técnicas Imunoenzimáticas
Estações do Ano
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glucocorticoids)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191961


  2 / 67370 MEDLINE  
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[PMID]:29203739
[Au] Autor:Berezniakova AI; Cheremisina VF
[Ad] Endereço:National Pharmaceutical University, Kharkov, Ukraine.
[Ti] Título:4 and 6 interleukin's action in the pathogenesis of periodontitis, gingivitis and dental alveolitis.
[So] Source:Wiad Lek;70(5):910-912, 2017.
[Is] ISSN:0043-5147
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The paper presents the results of studying the role of interleukins 4 and 6 in the pathogenesis of periodontal tissue diseases, specifically, in periodontitis, gingivitis and alveolitis. THE AIM: To study the nature of participation of IL-4 and IL-6 in the mechanisms of development of periodontitis, gingivitis and alveolitis. MATERIALS AND METHODS: Studies were carried out on 80 nonlinear male rats with a body weight of 200.0 to 220.0 g divided into four groups of 20 animals each. The serum level of cytokines was determined by an enzyme immunoassay on the Multiscane Biotech analyzer using test systems manufactured by Caltag laboratories (USA). Statistical processing of the obtained digital results was processed with the help of the program "Statistica 8.0". Indicators of the reliability of changes between the control and intact groups also used the Student's test and the Excel program. The confidence level was taken at p <0.05. RESULTS: As a result of our experiments, noticeable changes in the anti-inflammatory cytokine IL-4 were observed in rats with experimental periodontitis. The level of IL-4 cytokine in rats with alveolitis did not differ from control. The level of proinflammatory cytokine IL-6 from all groups of animals with periodontal disease differed from control only in rats with gingivitis, where it decreased by 74% and its level became less with alveolitis and periodontitis, since in these diseases the level of IL-6 was practically the same from the control (p <0,05). We also succeeded in revealing that at a low level of profibrogenic IL-6, there is not enough stimulation of collagen synthesis in the periodontal bone tissue. The increased level of IL-4 in a group of animals with gingivitis, on the contrary, indicates the realization of a pathological reaction of the organism. CONCLUSIONS: The change in the levels of pro- and anti-inflammatory interleukins, especially with gingivitis, indicates a decrease in the body's adaptive reserves and may affect the further dynamics of the inflammatory process in the periodontal tissues.
[Mh] Termos MeSH primário: Processo Alveolar/imunologia
Gengivite/imunologia
Interleucina-4/sangue
Interleucina-6/sangue
Doenças Periodontais/imunologia
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Técnicas Imunoenzimáticas
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-6); 207137-56-2 (Interleukin-4)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE


  3 / 67370 MEDLINE  
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[PMID]:27773873
[Au] Autor:Rourk AR; Nolte FS; Litwin CM
[Ti] Título:Performance Characteristics of the Reverse Syphilis Screening Algorithm in a Population With a Moderately High Prevalence of Syphilis.
[So] Source:Am J Clin Pathol;146(5):572-577, 2016 Nov 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: With the recent introduction of automated treponemal tests, a new reverse syphilis algorithm has been proposed and now used by many clinical laboratories. We analyzed the impact of instituting the reverse screening syphilis algorithm in a laboratory that serves a geographic area with a moderately high prevalence of syphilis infection. Methods: Serum samples sent for syphilis testing were tested using a treponemal enzyme immunoassay (EIA) as the screening assay. EIA reactive samples were tested by rapid plasma reagin (RPR) and titered to end point if reactive. RPR nonreactive samples were analyzed by the Treponema pallidum particle agglutination test (TP-PA). Pertinent medical records were reviewed for false-reactive screens and samples with evidence of past syphilis infection. Results: Among 10,060 patients tested, 502 (5%) were reactive on the initial EIA screen. The RPR was reactive in 150 (1.5%). TP-PA testing determined that 103 (1.0%) were falsely reactive on initial EIA screen. The reverse screening algorithm, however, identified 242 (2.4%) with evidence of latent, secondary, or past syphilis, 21 of whom had no or unknown prior treatment with antibiotics. Conclusions: Despite a 1.0% false-reactive rate, the reverse syphilis algorithm detected 21 patients with possible latent syphilis that may have gone undetected by traditional syphilis screening.
[Mh] Termos MeSH primário: Algoritmos
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Sífilis/diagnóstico
Treponema pallidum/imunologia
[Mh] Termos MeSH secundário: Reações Falso-Negativas
Seres Humanos
Técnicas Imunoenzimáticas
Imunoglobulina G/sangue
Prevalência
Sífilis/epidemiologia
Sífilis/microbiologia
Sorodiagnóstico da Sífilis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Immunoglobulin G)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqw182


  4 / 67370 MEDLINE  
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[PMID]:29370173
[Au] Autor:Benedé S; Berin MC
[Ad] Endereço:Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.
[Ti] Título:Mast cell heterogeneity underlies different manifestations of food allergy in mice.
[So] Source:PLoS One;13(1):e0190453, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Food can trigger a diverse array of symptoms in food allergic individuals from isolated local symptoms affecting skin or gut to multi-system severe reactions (systemic anaphylaxis). Although we know that gastrointestinal and systemic manifestations of food allergy are mediated by tissue mast cells (MCs), it is not clear why allergen exposure by the oral route can result in such distinct clinical manifestations. Our aim was to assess the contribution of mast cell subsets to different manifestations of food allergy. We used two common models of IgE-mediated food allergy, one resulting in systemic anaphylaxis and the other resulting in acute gastrointestinal symptoms, to study the immune basis of allergic reactions. We used responders and non-responders in each model system, as well as naïve controls to identify the association of mast cell activation with clinical reactivity rather than sensitization. Systemic anaphylaxis was uniquely associated with activation of connective tissue mast cells (identified by release of mouse mast cell protease (MMCP) -7 into the serum) and release of histamine, while activation of mucosal mast cells (identified by release of MMCP-1 in the serum) did not correlate with symptoms. Gastrointestinal manifestations of food allergy were associated with an increase of MMCP-1-expressing mast cells in the intestine, and evidence of both mucosal and connective tissue mast cell activation. The data presented in this paper demonstrates that mast cell heterogeneity is an important contributor to manifestations of food allergy, and identifies the connective tissue mast cell subset as key in the development of severe systemic anaphylaxis.
[Mh] Termos MeSH primário: Hipersensibilidade Alimentar/imunologia
Mastócitos/imunologia
[Mh] Termos MeSH secundário: Animais
Trato Gastrointestinal/imunologia
Liberação de Histamina
Técnicas Imunoenzimáticas
Mastócitos/enzimologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C3H
Reação em Cadeia da Polimerase em Tempo Real
Triptases/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
EC 3.4.21.59 (Tpsab1 protein, mouse); EC 3.4.21.59 (Tryptases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190453


  5 / 67370 MEDLINE  
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[PMID]:29195765
[Au] Autor:Walkty A; Keynan Y; Karlowsky J; Dhaliwal P; Embil J
[Ad] Endereço:Department of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada. Electronic address: AWalkty@dsmanitoba.ca.
[Ti] Título:Central nervous system blastomycosis diagnosed using the MVista® Blastomyces quantitative antigen enzyme immunoassay test on cerebrospinal fluid: A case report and review of the literature.
[So] Source:Diagn Microbiol Infect Dis;90(2):102-104, 2018 Feb.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blastomyces dermatitidis is a thermally dimorphic fungus that is capable of causing pulmonary and extra-pulmonary disease, including infections of the central nervous system (CNS). Diagnosis of CNS blastomycosis with non-invasive testing can be difficult, and a surgical biopsy may ultimately be required for microbiological and/or histopathological confirmation. A case of B. dermatitidis meningitis is presented where the diagnosis was made by testing cerebrospinal fluid (CSF) using the MVista® Blastomyces Quantitative Antigen Enzyme Immunoassay test. The utility of performing this test on CSF for diagnosis of CNS mass lesions/abscesses caused by B. dermatitidis in the absence of associated meningitis remains unclear. Cross reaction of the Blastomyces antigen test with other dimorphic fungi is a concern, necessitating that positive test results are interpreted in the context of the patient's exposure and travel history.
[Mh] Termos MeSH primário: Antígenos de Fungos/líquido cefalorraquidiano
Blastomicose/diagnóstico
Infecções Fúngicas do Sistema Nervoso Central/diagnóstico
Técnicas Imunoenzimáticas/métodos
[Mh] Termos MeSH secundário: Idoso
Blastomyces
Blastomicose/líquido cefalorraquidiano
Blastomicose/microbiologia
Infecções Fúngicas do Sistema Nervoso Central/líquido cefalorraquidiano
Infecções Fúngicas do Sistema Nervoso Central/microbiologia
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, Fungal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


  6 / 67370 MEDLINE  
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[PMID]:29374725
[Au] Autor:Suzuki K; Watanabe A; Araki K; Yokobori T; Harimoto N; Gantumur D; Hagiwara K; Yamanaka T; Ishii N; Tsukagoshi M; Igarashi T; Kubo N; Gombodorj N; Nishiyama M; Hosouchi Y; Kuwano H; Shirabe K
[Ad] Endereço:Department of Hepatobiliary and Pancreatic Surgery, Gunma University, Graduate School of Medicine, Maebashi, Japan.
[Ti] Título:High STMN1 Expression Is Associated with Tumor Differentiation and Metastasis in Clinical Patients with Pancreatic Cancer.
[So] Source:Anticancer Res;38(2):939-944, 2018 02.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer-related deaths worldwide. Stathmin 1 (STMN1) suppression was reported to reduce cellular viability and migration potential. However, no previous studies have addressed whether STMN1 overexpression is associated with malignant potential in PDAC. MATERIALS AND METHODS: To clarify the clinical significance of STMN1 in PDAC, the STMN1 expression in 104 PDAC samples was evaluated by immunohistochemistry. Moreover, we evaluated the proliferative potential and migration ability of pancreatic cancer cell line Suit2 cells highly expressing STMN1. RESULTS: Cytoplasmic STMN1 were higher levels in PDAC than in corresponding non-cancerous tissues. PDAC patients with high STMN1 (n=29) were significantly associated with poor differentiation and distant metastasis compared to those with low STMN1 (n=75). The proliferation rates and migration ability in Suit2-STMN1 were higher than those of Suit2-mock. CONCLUSION: STMN1 evaluation could be a useful progression marker, and STMN1 may be a promising candidate for targeted therapies in PDAC.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Carcinoma Ductal Pancreático/secundário
Diferenciação Celular
Movimento Celular
Regulação Neoplásica da Expressão Gênica
Neoplasias Pancreáticas/patologia
Estatmina/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/genética
Carcinoma Ductal Pancreático/genética
Carcinoma Ductal Pancreático/metabolismo
Estudos de Casos e Controles
Proliferação Celular
Progressão da Doença
Feminino
Seguimentos
Seres Humanos
Técnicas Imunoenzimáticas
Metástase Linfática
Masculino
Meia-Idade
Pâncreas/metabolismo
Neoplasias Pancreáticas/genética
Neoplasias Pancreáticas/metabolismo
Prognóstico
Estatmina/genética
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (STMN1 protein, human); 0 (Stathmin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180129
[St] Status:MEDLINE


  7 / 67370 MEDLINE  
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[PMID]:27739315
[Au] Autor:Sedláková I; Laco J; Tosner J; Spacek J
[Ti] Título:[Expression of ATP-binding Cassette Proteins Pgp, MRP1, and MRP3 in Malignant and Benign Ovarian Lesions].
[Ti] Título:Exprese ABC transportéru Pgp, MRP1 a MRP3 u maligních a benigních zmen vajecníku..
[So] Source:Klin Onkol;29(5):358-363, 2016.
[Is] ISSN:0862-495X
[Cp] País de publicação:Czech Republic
[La] Idioma:cze
[Ab] Resumo:BACKGROUND: This study was designed to compare the expression of PgP (P-glycoprotein), MRP1 (multidrug related protein), and MRP3 in ovarian cancer patients, patients with benign ovarian tumors, and healthy women, and to evaluate the correlation between the expression of ATP-binding cassette proteins Pgp, MRP1, and MRP3 with stage, grade, and histological type. PATIENTS AND METHODS: Tissue specimens from 212 women who underwent surgery at the Department of Obstetrics and Gynecology at University Hospital Hradec Králové were subjected to immunohistochemical staining for Pgp, MRP1, and MRP3. RESULTS: The expression of Pgp and MRP1 was higher in ovarian tumor cells than in the cells lining the ovarian cyst. The lowest level of expression was found in normal ovarian tissue (p < 0.001). Histological subtype of epithelial ovarian cancer correlated with the expression of PgP, MRP1, and MRP3. The lowest level of Pgp and MRP1 expression was found in endometrioid ovarian cancers (p = 0.151; p = 0.013). Patients with advanced ovarian cancer (FIGO III + IV) had higher MRP1 expression than those with early stage ovarian cancer (median MRP1 FIGO I + II 80%; CI 60-100; FIGO III + IV 100%; CI 90-100; p = 0.100). An association was observed between MRP1 and tumor grade (p < 0.001). CONCLUSION: Pgp and MRP1 expression was higher in ovarian tumor cells than in cells lining the ovarian cyst. The lowest level of expression was found in normal ovarian tissue. ATP-binding cassette proteins play an important role in ovarian cancer pathogenesis.Key words: ATP-binding cassette proteins - ovarian cancer - P-glycoprotein (Pgp) - multidrug related protein 1 (MRP1) - multidrug related protein 3 (MRP3) - drug resistanceThis work was supported by the Czech Ministry of Health NT 14107-3/2013.The authors declare they have no potential confl icts of interest concerning drugs, products, or services used in the study.The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers.Submitted: 9. 11. 2015Accepted: 30. 8. 2016.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Neoplasias do Endométrio/metabolismo
Proteínas Associadas à Resistência a Múltiplos Medicamentos/metabolismo
Neoplasias Ovarianas/metabolismo
[Mh] Termos MeSH secundário: Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo
Neoplasias do Endométrio/patologia
Neoplasias do Endométrio/cirurgia
Feminino
Seres Humanos
Técnicas Imunoenzimáticas
Neoplasias Ovarianas/patologia
Neoplasias Ovarianas/cirurgia
Ovário/metabolismo
Ovário/patologia
Prognóstico
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCB1 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (Biomarkers, Tumor); 0 (Multidrug Resistance-Associated Proteins); 1YV0492L5Z (multidrug resistance-associated protein 3); Y49M64GZ4Q (multidrug resistance-associated protein 1)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161015
[St] Status:MEDLINE


  8 / 67370 MEDLINE  
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[PMID]:27773698
[Au] Autor:Duan L; Davis JS; Woolbright BL; Du K; Cahkraborty M; Weemhoff J; Jaeschke H; Bourdi M
[Ad] Endereço:Department of Pharmacology, Toxicology & Therapeutics, Kansas City, KS, 66160, USA. Electronic address: lduan@kumc.edu.
[Ti] Título:Differential susceptibility to acetaminophen-induced liver injury in sub-strains of C57BL/6 mice: 6N versus 6J.
[So] Source:Food Chem Toxicol;98(Pt B):107-118, 2016 Dec.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mouse models of acetaminophen (APAP) hepatotoxicity are considered relevant for the human pathophysiology. The C57BL/6 strain is most popular because it is the background strain of gene knock-out mice. However, conflicting results in the literature may have been caused by sub-strain mismatches, e.g. C57BL/6J and C57BL/6N. This study was initiated to determine the mechanism behind the sub-strain susceptibility to APAP toxicity. C57BL/6N and C57BL/6J mice were dosed with 200 mg/kg APAP and sacrificed at different time points. C57BL/6N mice developed significantly more liver injury as measured by plasma ALT activities and histology. Although there was no difference in glutathione depletion or cytochrome P450 activity between groups, C57BL/6N had a higher glutathione disulfide-to-glutathione ratio and more APAP protein adducts. C57BL/6N showed more mitochondrial translocation of phospho-JNK and BAX, and more release of mitochondrial intermembrane proteins apoptosis-inducing factor (AIF), second mitochondria-derived activator of caspases (SMAC), which caused more DNA fragmentation. The increased mitochondrial dysfunction was confirmed in vitro as C57BL/6N hepatocytes had a more precipitous drop in JC-1 fluorescence after APAP exposure. CONCLUSION: C57BL/6N mice are more susceptible to APAP-induced hepatotoxicity, likely due to increased formation of APAP-protein adducts and a subsequent enhancement of mitochondrial dysfunction associated with aggravated nuclear DNA fragmentation.
[Mh] Termos MeSH primário: Acetaminofen/toxicidade
Doença Hepática Induzida por Substâncias e Drogas/etiologia
Mitocôndrias Hepáticas/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Western Blotting
Doença Hepática Induzida por Substâncias e Drogas/patologia
Suscetibilidade a Doenças
Glutationa/metabolismo
Seres Humanos
Técnicas Imunoenzimáticas
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Mitocôndrias Hepáticas/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
362O9ITL9D (Acetaminophen); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  9 / 67370 MEDLINE  
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[PMID]:29023489
[Au] Autor:Biasutti S; Dart A; Smith M; Blaker C; Clarke E; Jeffcott L; Little C
[Ad] Endereço:Research and Clinical Training Unit, University Veterinary Teaching Hospital, University of Sydney, Camden, Australia.
[Ti] Título:Spatiotemporal variations in gene expression, histology and biomechanics in an ovine model of tendinopathy.
[So] Source:PLoS One;12(10):e0185282, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flexor tendinopathy is a common problem affecting humans and animals. Tendon healing is poorly understood and the outcomes of conservative and surgical management are often suboptimal. While often considered a localized injury, recent evidence indicates that in the short term, tendinopathic changes are distributed widely throughout the tendon, remote from the lesion itself. Whether these changes persist throughout healing is unknown. The aim of this study was to document gene expression, histopathological and biomechanical changes that occur throughout the superficial digital flexor tendon (SDFT) up to 16 weeks post-injury, using an ovine surgical model of tendinopathy. Partial tendon transection was associated with decreased gene expression for aggrecan, decorin, fibromodulin, tissue inhibitors of metalloproteinases (TIMPS 1, 2 and 3), collagen I and collagen II. Gene expression for collagen III, lumican and matrix metalloproteinase 13 (MMP13) increased locally around the lesion site. Expression of collagen III and MMP13 decreased with time, but compared to controls, collagen III, MMP13 and lumican expression remained regionally high throughout the study. An increase in TIMP3 was observed over time. Histologically, operated tendons had higher pathology scores than controls, especially around the injured region. A chondroid phenotype was observed with increased cellular rounding and marked proteoglycan accumulation which only partially improved with time. Biomechanically, partial tendon transection resulted in a localized decrease in elastic modulus (in compression) but only at 8 weeks postoperatively. This study improves our understanding of tendon healing, demonstrating an early 'peak' in pathology characterized by altered gene expression and notable histopathological changes. Many of these pathological changes become more localized to the region of injury during healing. Collagen III and MMP13 expression levels remained high close to the lesion throughout the study and may reflect the production of tendon tissue with suboptimal biomechanical properties. Further studies evaluating the long-term response of tendon to injury (6-12 months) are warranted to provide additional information on tendon healing and provide further understanding of the mechanisms underlying the pathology observed in this study.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Modelos Animais de Doenças
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Análise Espaço-Temporal
Tendinopatia/genética
Tendões/metabolismo
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Técnicas Imunoenzimáticas
Masculino
Ovinos
Tendinopatia/metabolismo
Tendinopatia/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185282


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[PMID]:28973595
[Au] Autor:Miceli MH; Kauffman CA
[Ad] Endereço:Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
[Ti] Título:Aspergillus Galactomannan for Diagnosing Invasive Aspergillosis.
[So] Source:JAMA;318(12):1175-1176, 2017 Sep 26.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Aspergillus fumigatus
Líquido da Lavagem Broncoalveolar/química
Hospedeiro Imunocomprometido
Técnicas Imunoenzimáticas
Aspergilose Pulmonar Invasiva/diagnóstico
Mananas/análise
[Mh] Termos MeSH secundário: Idoso
Aspergillus fumigatus/isolamento & purificação
Biópsia
Líquido da Lavagem Broncoalveolar/microbiologia
Granulomatose com Poliangiite/complicações
Granulomatose com Poliangiite/tratamento farmacológico
Seres Humanos
Masculino
Mananas/sangue
Sensibilidade e Especificidade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannans); 11078-30-1 (galactomannan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.10661



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