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  1 / 46022 MEDLINE  
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[PMID]:28749335
[Au] Autor:Larsen J; Sunde M; Islam MZ; Urdahl AM; Barstad AS; Larsen AR; Grøntvedt CA; Angen Ø
[Ad] Endereço:Statens Serum Institut, Copenhagen S, Denmark.
[Ti] Título:Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016.
[So] Source:Euro Surveill;22(28), 2017 Jul 13.
[Is] ISSN:1560-7917
[Cp] País de publicação:Sweden
[La] Idioma:eng
[Ab] Resumo:We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.
[Mh] Termos MeSH primário: Técnicas de Cultura/métodos
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Infecções Estafilocócicas/diagnóstico
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Animais
Dinamarca
Gado
Noruega
Sensibilidade e Especificidade
Infecções Estafilocócicas/veterinária
Sus scrofa
Suínos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE


  2 / 46022 MEDLINE  
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[PMID]:27772619
[Au] Autor:Abad CL; Razonable RR
[Ad] Endereço:Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN.
[Ti] Título:α Herpes Virus Infections Among Renal Transplant Recipients.
[So] Source:Semin Nephrol;36(5):344-350, 2016 09.
[Is] ISSN:1558-4488
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The α herpes viruses HSV-1, HSV-2, and VZV often reactivate in the setting of immune suppression after solid organ transplantation. Oral or genital mucocutaneous disease is the most common clinical manifestation of HSV disease while VZV manifests as varicella (or chickenpox) or reactivation herpes zoster, characterized by a diffuse rash, or a painful unilateral vesicular eruption in a dermatomal distribution, respectively. The diagnosis of HSV and VZV is primarily based on history and clinical presentation, although diagnostic tests may be necessary for atypical presentations of disease. Treatment usually involves oral or intravenous antiviral therapy, depending on severity of illness.
[Mh] Termos MeSH primário: Varicela/induzido quimicamente
Rejeição de Enxerto/prevenção & controle
Herpes Simples/induzido quimicamente
Herpes Zoster/induzido quimicamente
Imunossupressores/efeitos adversos
Falência Renal Crônica/cirurgia
Transplante de Rim
[Mh] Termos MeSH secundário: Antivirais/uso terapêutico
Varicela/diagnóstico
Varicela/tratamento farmacológico
Varicela/prevenção & controle
Vacina contra Varicela/uso terapêutico
Técnicas de Cultura
Técnica Direta de Fluorescência para Anticorpo
Herpes Simples/diagnóstico
Herpes Simples/tratamento farmacológico
Herpes Zoster/diagnóstico
Herpes Zoster/tratamento farmacológico
Herpes Zoster/prevenção & controle
Herpesvirus Humano 1
Herpesvirus Humano 2
Herpesvirus Humano 3
Seres Humanos
Reação em Cadeia da Polimerase
Testes Sorológicos
Ativação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Chickenpox Vaccine); 0 (Immunosuppressive Agents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  3 / 46022 MEDLINE  
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[PMID]:28471044
[Au] Autor:Villano M; Paiano P; Palma E; Miccheli A; Majone M
[Ad] Endereço:Department of Chemistry, Sapienza University of Rome, P. le Aldo Moro 5, 00185, Rome, Italy.
[Ti] Título:Electrochemically Driven Fermentation of Organic Substrates with Undefined Mixed Microbial Cultures.
[So] Source:ChemSusChem;10(15):3091-3097, 2017 08 10.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Growing scientific interest in mixed microbial culture-based anaerobic biotechnologies for the production of value-added chemicals and fuels from organic waste residues requires a parallel focus on the development and implementation of strategies to control the distribution of products. This study examined the feasibility of an electrofermentation approach, based on the introduction of a polarized (-700 mV vs. the standard hydrogen electrode) graphite electrode in the fermentation medium, to steer the product distribution during the conversion of organic substrates (glucose, ethanol, and acetate supplied as single compounds or in mixtures) by undefined mixed microbial cultures. In batch experiments, the polarized electrode triggered a nearly 20-fold increase (relative to open circuit controls) in the yield of isobutyrate production (0.43±0.01 vs. 0.02±0.02 mol mol glucose) during the anaerobic fermentation of the ternary mixture of substrates, without adversely affecting the rate of substrate bioconversion. The observed change in the fermentative metabolism was most likely triggered by the (potentiostatic) regulation of the oxidation-reduction potential of the reaction medium rather than by the electrode serving as an electron donor.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica/microbiologia
Fermentação
Compostos Orgânicos/metabolismo
[Mh] Termos MeSH secundário: Técnicas de Cultura
Eletroquímica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Organic Chemicals)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201700360


  4 / 46022 MEDLINE  
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[PMID]:28818779
[Au] Autor:Swathy PS; Rupal G; Prabhu V; Mahato KK; Muthusamy A
[Ad] Endereço:Department of Plant Sciences, School of Life Sciences, Manipal University, Manipal, Karnataka, India.
[Ti] Título:In vitro culture responses, callus growth and organogenetic potential of brinjal (Solanum melongena L.) to He-Ne laser irradiation.
[So] Source:J Photochem Photobiol B;174:333-341, 2017 Sep.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present investigation was designed to analyze the influence of Helium-Neon (He-Ne 632.8nm) laser irradiation on defense enzymes, proline content and in vitro responses of callus induction, shoot initiation and on plantlet regeneration potential of brinjal. The seeds of Mattu Gulla (Solanum melongena L.) were irradiated with 20, 25 and 30J/cm of He-Ne laser followed by surface sterilization and sprouted on Murashige and Skoog medium without plant growth regulators. The activity of defense enzymes, proline content and the organogenetic potential of hypocotyl, leaf and shoot tip explants were determined from thirty day old seedlings. During seed germination, most of the seedlings showed normal two cotyledons whereas small number of seedlings showed tricotyledonous at 20J/cm treatment and no other morphological abnormalities were observed during further growth and development. There was no substantial variation was noted in both ß-1,3-glucanase and chitinase activity as well as proline content which proves the He-Ne laser irradiation does not causes any stresses for the plant. The in vitro culture of hypocotyl, leaf and shoot tip explants from laser irradiated seedlings showed differential responses as compared to un-irradiated control. The laser induced enhancement of callus induction, growth rate of callus tissues and shoot tip, percentage of responses of shoot and root initiation, days to shoot and root initiation, shoots formed per callus, number of roots per shoots, length of roots and nuclear DNA content of in vitro raised plants were evaluated. Among the tested laser doses (20, 25 and 30J/cm ), 25J/cm showed significant biostimulatory effect over un-irradiated control seedlings. The present observations reveal and endorsed our earlier reports with substantial enhancement of in vitro and ex vitro by He-Ne laser irradiation.
[Mh] Termos MeSH primário: Lasers
Solanum melongena/crescimento & desenvolvimento
Solanum melongena/efeitos da radiação
[Mh] Termos MeSH secundário: Núcleo Celular/genética
Núcleo Celular/efeitos da radiação
Técnicas de Cultura
DNA de Plantas/metabolismo
Germinação/efeitos da radiação
Organogênese/efeitos da radiação
Prolina/metabolismo
Plântulas/crescimento & desenvolvimento
Plântulas/efeitos da radiação
Solanum melongena/citologia
Solanum melongena/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 9DLQ4CIU6V (Proline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


  5 / 46022 MEDLINE  
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[PMID]:28812427
[Au] Autor:Furumoto T; Sato R
[Ad] Endereço:a Faculty of Agriculture, Department of Applied Biological Science , Kagawa University , Kagawa , Japan.
[Ti] Título:Biosynthesis of anthraquinone derivatives in a Sesamum indicum hairy root culture.
[So] Source:Biosci Biotechnol Biochem;81(10):1868-1873, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In order to investigate the intermediacy of 2-(4-methylpent-3-en-1-yl)anthraquinone (MPAQ), a possible intermediate for the biosynthesis of anthraquinone derivatives in sesame (Sesamum indicum), H-labeled MPAQ was administered to a hairy root culture of S. indicum. Efficient conversion of fed MPAQ to 2-[(Z)-4-methylpenta-1,3-dien-1-yl]anthraquinone ((Z)-MPDEAQ) was observed. Furthermore, administration experiment with H-labeled 2-geranyl-1,4-naphthohydroquinone, another possible intermediate, showed that it was converted to MPAQ and (Z)-MPDEAQ. The results clearly demonstrated that these substrates are the actual precursors for the production of (Z)-MPDEAQ. In contrast, neither MPAQ nor 2-geranyl-1,4-naphthohydroquinone was converted to anthrasesamone B and 2,3-epoxyanthrasesamone B, other anthraquinone derivatives in the hairy roots, suggesting that these substrates may not be the common precursors in the biosynthesis of anthraquinone derivatives.
[Mh] Termos MeSH primário: Antraquinonas/metabolismo
Raízes de Plantas/crescimento & desenvolvimento
Sesamum/crescimento & desenvolvimento
Sesamum/metabolismo
[Mh] Termos MeSH secundário: Técnicas de Cultura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthraquinones); 030MS0JBDO (9,10-anthraquinone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1362974


  6 / 46022 MEDLINE  
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[PMID]:28782454
[Au] Autor:Gotoh A; Nara M; Sugiyama Y; Sakanaka M; Yachi H; Kitakata A; Nakagawa A; Minami H; Okuda S; Katoh T; Katayama T; Kurihara S
[Ad] Endereço:a Research Institute for Bioresources and Biotechnology , Ishikawa Prefectural University , Nonoichi , Japan.
[Ti] Título:Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles.
[So] Source:Biosci Biotechnol Biochem;81(10):2009-2017, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.
[Mh] Termos MeSH primário: Bactérias/crescimento & desenvolvimento
Bactérias/metabolismo
Ácidos Graxos Voláteis/metabolismo
Fermentação
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Anaerobiose
Bactérias/genética
Simulação por Computador
Técnicas de Cultura
DNA Bacteriano/genética
Genômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids, Volatile)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1359486


  7 / 46022 MEDLINE  
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[PMID]:28682896
[Au] Autor:Zaiyou J; Li M; Xiqiao H
[Ad] Endereço:aInstitute of Science and Technology, Xinxiang, Henan Province bHenan Collaborative Innovation Center of Modern Biological Breeding, Xinxiang. China.
[Ti] Título:An endophytic fungus efficiently producing paclitaxel isolated from Taxus wallichiana var. mairei.
[So] Source:Medicine (Baltimore);96(27):e7406, 2017 Jul.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Paclitaxel is a medicinal ingredient with high anticancer activity and widely used in hospitals and clinics. In this study, we isolate endophytic fungi efficiently producing paclitaxel from yew for the purpose of paclitaxel manufacture.The bark of Taxus wallichiana var. mairei was surface sterilized and then inoculated in potato dextrose agar culture medium to isolate endophytic fungi. The paclitaxel in the fungal culture was extracted with mixture of chloroform and the same amount of methanol. The content of paclitaxel in the extract was determined and identified with LC-MS. The endophytic fungus efficiently producing paclitaxel was species identified with ITS rDNA and 26S D1/D2 rDNA sequencing.There were 528 endophytic fungal strains were isolated from the bark of T wallichiana var. mairei in total. There was only a strain efficiently producing paclitaxel in these endophytic fungi. The unique strain was identified as Phoma medicaginis. The paclitaxel contents in whole potato dextrose broth (PDB) culture, spent culture medium from this strain and that in dry mycelium is 1.215 mg/L, 0.936 mg/L, and 20 mg/kg, respectively.An endophytic fungus efficiently producing paclitaxel was isolated from T wallichiana var. mairei. This isolated endophytic fungus can be used as a producing strain for paclitaxel manufacture.
[Mh] Termos MeSH primário: Endófitos/isolamento & purificação
Endófitos/metabolismo
Fungos/isolamento & purificação
Fungos/metabolismo
Paclitaxel/biossíntese
Taxus/microbiologia
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Meios de Cultura
Técnicas de Cultura
Glucose
Melhoria de Qualidade
Solanum tuberosum
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); IY9XDZ35W2 (Glucose); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007406


  8 / 46022 MEDLINE  
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[PMID]:28493770
[Au] Autor:Davis C; Rantell A
[Ad] Endereço:Clinical Nurse Specialist in Urogynaecology, King's College Hospital, London.
[Ti] Título:Lower urinary tract infections in women.
[So] Source:Br J Nurs;26(9):S12-S19, 2017 May 11.
[Is] ISSN:0966-0461
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In her lifetime, a woman is highly likely to develop at least one lower urinary tract infection. Early detection and treatment are key. Being aware of predisposing factors for infection and understanding appropriate diagnosis and treatment regimens will help nurses in both primary and acute care manage these patients correctly. This will not only benefit patients but will also help prevent incorrect antimicrobial management and avoid unplanned admissions. This aim of this article is to provide nurses with the information they need to best advise both colleagues and patients on how to manage lower urinary tract infections in women.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Infecções Assintomáticas
Farmacorresistência Bacteriana
Infecções Urinárias/tratamento farmacológico
[Mh] Termos MeSH secundário: Fatores Etários
Anti-Infecciosos Urinários/uso terapêutico
Vaginite Atrófica/tratamento farmacológico
Vaginite Atrófica/epidemiologia
Bacteriúria/diagnóstico
Bacteriúria/tratamento farmacológico
Bacteriúria/prevenção & controle
Bacteriúria/urina
Anticoncepção
Técnicas de Cultura
Terapia de Reposição de Estrogênios
Feminino
Hipuratos/uso terapêutico
Seres Humanos
Metenamina/análogos & derivados
Metenamina/uso terapêutico
Nitritos/urina
Educação de Pacientes como Assunto
Probióticos/uso terapêutico
Recidiva
Fatores de Risco
Comportamento Sexual
Infecções Urinárias/diagnóstico
Infecções Urinárias/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents, Urinary); 0 (Hippurates); 0 (Nitrites); J50OIX95QV (Methenamine); M329791L57 (methenamine hippurate)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.12968/bjon.2017.26.9.S12


  9 / 46022 MEDLINE  
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[PMID]:28426643
[Au] Autor:Marder EP; Cieslak PR; Cronquist AB; Dunn J; Lathrop S; Rabatsky-Ehr T; Ryan P; Smith K; Tobin-D'Angelo M; Vugia DJ; Zansky S; Holt KG; Wolpert BJ; Lynch M; Tauxe R; Geissler AL
[Ti] Título:Incidence and Trends of Infections with Pathogens Transmitted Commonly Through Food and the Effect of Increasing Use of Culture-Independent Diagnostic Tests on Surveillance - Foodborne Diseases Active Surveillance Network, 10 U.S. Sites, 2013-2016.
[So] Source:MMWR Morb Mortal Wkly Rep;66(15):397-403, 2017 Apr 21.
[Is] ISSN:1545-861X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Foodborne diseases represent a substantial public health concern in the United States. CDC's Foodborne Diseases Active Surveillance Network (FoodNet) monitors cases reported from 10 U.S. sites* of laboratory-diagnosed infections caused by nine enteric pathogens commonly transmitted through food. This report describes preliminary surveillance data for 2016 on the nine pathogens and changes in incidences compared with 2013-2015. In 2016, FoodNet identified 24,029 infections, 5,512 hospitalizations, and 98 deaths caused by these pathogens. The use of culture-independent diagnostic tests (CIDTs) by clinical laboratories to detect enteric pathogens has been steadily increasing since FoodNet began surveying clinical laboratories in 2010 (1). CIDTs complicate the interpretation of FoodNet surveillance data because pathogen detection could be affected by changes in health care provider behaviors or laboratory testing practices (2). Health care providers might be more likely to order CIDTs because these tests are quicker and easier to use than traditional culture methods, a circumstance that could increase pathogen detection (3). Similarly, pathogen detection could also be increasing as clinical laboratories adopt DNA-based syndromic panels, which include pathogens not often included in routine stool culture (4,5). In addition, CIDTs do not yield isolates, which public health officials rely on to distinguish pathogen subtypes, determine antimicrobial resistance, monitor trends, and detect outbreaks. To obtain isolates for infections identified by CIDTs, laboratories must perform reflex culture ; if clinical laboratories do not, the burden of culturing falls to state public health laboratories, which might not be able to absorb that burden as the adoption of these tests increases (2). Strategies are needed to preserve access to bacterial isolates for further characterization and to determine the effect of changing trends in testing practices on surveillance.
[Mh] Termos MeSH primário: Testes Diagnósticos de Rotina/métodos
Testes Diagnósticos de Rotina/utilização
Microbiologia de Alimentos
Parasitologia de Alimentos
Doenças Transmitidas por Alimentos/diagnóstico
Doenças Transmitidas por Alimentos/epidemiologia
Vigilância da População
[Mh] Termos MeSH secundário: Técnicas de Cultura/utilização
Seres Humanos
Incidência
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.15585/mmwr.mm6615a1


  10 / 46022 MEDLINE  
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[PMID]:28419503
[Au] Autor:Teramura H; Sota K; Iwasaki M; Ogihara H
[Ad] Endereço:Yokohama Research Center, JNC Corporation, Yokohama, Japan.
[Ti] Título:Comparison of the quantitative dry culture methods with both conventional media and most probable number method for the enumeration of coliforms and Escherichia coli/coliforms in food.
[So] Source:Lett Appl Microbiol;65(1):57-65, 2017 Jul.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sanita-kun™ CC (coliform count) and EC (Escherichia coli/coliform count), sheet quantitative culture systems which can avoid chromogenic interference by lactase in food, were evaluated in comparison with conventional methods for these bacteria. Based on the results of inclusivity and exclusivity studies using 77 micro-organisms, sensitivity and specificity of both Sanita-kun™ met the criteria for ISO 16140. Both media were compared with deoxycholate agar, violet red bile agar, Merck Chromocult™ coliform agar (CCA), 3M Petrifilm™ CC and EC (PEC) and 3-tube MPN, as reference methods, in 100 naturally contaminated food samples. The correlation coefficients of both Sanita-kun™ for coliform detection were more than 0·95 for all comparisons. For E. coli detection, Sanita-kun™ EC was compared with CCA, PEC and MPN in 100 artificially contaminated food samples. The correlation coefficients for E. coli detection of Sanita-kun™ EC were more than 0·95 for all comparisons. There were no significant differences in all comparisons when conducting a one-way analysis of variance (anova). Both Sanita-kun™ significantly inhibited colour interference by lactase when inhibition of enzymatic staining was assessed using 40 natural cheese samples spiked with coliform. Our results demonstrated Sanita-kun™ CC and EC are suitable alternatives for the enumeration of coliforms and E. coli/coliforms, respectively, in a variety of foods, and specifically in fermented foods. SIGNIFICANCE AND IMPACT OF THE STUDY: Current chromogenic media for coliforms and Escherichia coli/coliforms have enzymatic coloration due to breaking down of chromogenic substrates by food lactase. The novel sheet culture media which have film layer to avoid coloration by food lactase have been developed for enumeration of coliforms and E. coli/coliforms respectively. In this study, we demonstrated these media had comparable performance with reference methods and less interference by food lactase. These media have a possibility not only to be useful alternatives but also to contribute for accurate enumeration of these bacteria in a variety of foods, and specifically in fermented foods.
[Mh] Termos MeSH primário: Contagem de Colônia Microbiana/métodos
Meios de Cultura/química
Escherichia coli/crescimento & desenvolvimento
Contaminação de Alimentos/análise
Microbiologia de Alimentos/métodos
[Mh] Termos MeSH secundário: Ágar/química
Técnicas de Cultura
Lactase/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 9002-18-0 (Agar); EC 3.2.1.108 (Lactase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12744



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