Base de dados : MEDLINE
Pesquisa : E05.481.500.484 [Categoria DeCS]
Referências encontradas : 24310 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2431 ir para página                         

  1 / 24310 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29223396
[Au] Autor:Matsuishi YI; Kato H; Masuda K; Yamaza H; Hirofuji Y; Sato H; Wada H; Kiyoshima T; Nonaka K
[Ad] Endereço:Section of Oral Medicine for Children, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.
[Ti] Título:Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1.
[So] Source:Biochem Biophys Res Commun;495(2):1655-1660, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Undifferentiated odontogenic epithelium and dental papilla cells differentiate into ameloblasts and odontoblasts, respectively, both of which are essential for tooth development. These differentiation processes involve dramatic functional and morphological changes of the cells. For these changes to occur, activation of mitochondrial functions, including ATP production, is extremely important. In addition, these changes are closely related to mitochondrial fission and fusion, known as mitochondrial dynamics. However, few studies have focused on the role of mitochondrial dynamics in tooth development. The purpose of this study was to clarify this role. We used mouse tooth germ organ cultures and a mouse dental papilla cell line with the ability to differentiate into odontoblasts, in combination with knockdown of the mitochondrial fission factor, dynamin related protein (DRP)1. In organ cultures of the mouse first molar, tooth germ developed to the early bell stage. The amount of dentin formed under DRP1 inhibition was significantly larger than that of the control. In experiments using a mouse dental papilla cell line, differentiation into odontoblasts was enhanced by inhibiting DRP1. This was associated with increased mitochondrial elongation and ATP production compared to the control. These results suggest that DRP1 inhibition accelerates dentin formation through mitochondrial elongation and activation. This raises the possibility that DRP1 might be a therapeutic target for developmental disorders of teeth.
[Mh] Termos MeSH primário: Dentinogênese/fisiologia
Dinaminas/antagonistas & inibidores
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/biossíntese
Ameloblastos/citologia
Ameloblastos/fisiologia
Animais
Diferenciação Celular/genética
Diferenciação Celular/fisiologia
Linhagem Celular
Dinaminas/genética
Dinaminas/fisiologia
Proteínas da Matriz Extracelular/biossíntese
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Dinâmica Mitocondrial/fisiologia
Odontoblastos/citologia
Odontoblastos/fisiologia
Técnicas de Cultura de Órgãos
Fosfoproteínas/biossíntese
Gravidez
RNA Interferente Pequeno/genética
Sialoglicoproteínas/biossíntese
Germe de Dente/citologia
Germe de Dente/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Phosphoproteins); 0 (RNA, Small Interfering); 0 (Sialoglycoproteins); 0 (dentin sialophosphoprotein); 8L70Q75FXE (Adenosine Triphosphate); EC 3.6.5.5 (Dnm1l protein, mouse); EC 3.6.5.5 (Dynamins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE


  2 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28463660
[Au] Autor:Jimi S; Miyazaki M; Takata T; Ohjimi H; Akita S; Hara S
[Ad] Endereço:1​Central Laboratory for Pathology and Morphology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
[Ti] Título:Increased drug resistance of meticillin-resistant Staphylococcus aureus biofilms formed on a mouse dermal chip model.
[So] Source:J Med Microbiol;66(4):542-550, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODOLOGY: We established a novel tissue substrate model, namely MRSA grown on segments of mouse skin tissue (dermal chips, DCs), and compared its resistance capacity against VCM with that of MRSA biofilms grown on plastic chips (PCs).Results/Key findings. For one MRSA isolate, we found that the VCM MIC was identical (1.56 µg ml-1) for planktonic cultures and for biofilms-formed on PCs (PC-BF), although the minimum bactericidal concentration (MBC) increased to 6.25 µg ml-1 in PC-BF. On the contrary, the MIC and MBC for biofilms formed on DCs (DC-BF) significantly increased (25 and 50 µg ml-1, respectively). Furthermore, the minimum biofilm-eradicating concentration was higher for DC-BF (100 µg ml-1) than for PC-BF (25 µg ml-1). Using six MRSA strains, we found that in PC-BF, the c.f.u. number decreased with increasing VCM concentration, whereas in DC-BF, it greatly increased until the MIC was reached, accompanied by the formation of large colonies, thicker bacterial walls and the presence of many mitotic cells. CONCLUSION: Our results indicate that the VCM resistance of MRSA was greater in DC-BF. We conclude that DCs may provide a specific environment for MRSA that enhances bacterial growth under cytotoxic VCM concentrations, and might be useful for the study of skin wound infections and the effects of antimicrobial drugs.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Resistência a Vancomicina/fisiologia
Vancomicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Feminino
Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Camundongos
Camundongos Endogâmicos C57BL
Testes de Sensibilidade Microbiana
Técnicas de Cultura de Órgãos
Pele/microbiologia
Infecções Estafilocócicas/tratamento farmacológico
Infecções Estafilocócicas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 6Q205EH1VU (Vancomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000461


  3 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29331377
[Au] Autor:Jeong WY; Yoo HY; Kim CW
[Ad] Endereço:Department of Biotechnology, BK21 Plus Program, College of Life Sciences and Biotechnology, Korea University, 1-5, Anam Dong, Seongbuk-Gu, Seoul 136-701, South Korea.
[Ti] Título:ß-cellulin promotes the proliferation of corneal epithelial stem cells through the phosphorylation of erk1/2.
[So] Source:Biochem Biophys Res Commun;496(2):359-366, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The proliferation of corneal epithelial stem cells (CESCs) is a very important process in the recovery of corneal wounds. Recent studies have shown that ß-cellulin (BC) is effective in the repair of other tissues. However, its mechanism of action in corneal wound healing is not yet clear. The purpose of this study was to investigate how BC accelerates wound healing of the cornea. Here, we confirmed that the proliferation of CESCs was induced at a specific concentration (0.2, 2 and 20 ng/mL) by treatment with BC. Markers associated with proliferation activity (ΔNp63, bmi-1, abcg2) were also upregulated. In vivo experiments showed that the corneal wound healing rate was increased in mice. We found that BC stimulates the phosphorylation of the erk1/2 signaling pathway, which is triggered during the recovery of mouse corneal wounds. However, the inhibition of erk1/2 phosphorylation delayed the recovery of mouse corneal wounds in an organ culture assay. According to these results, BC may be a potential treatment factor for corneal wound healing.
[Mh] Termos MeSH primário: Betacelulina/farmacologia
Células Epiteliais/efeitos dos fármacos
Epitélio Anterior/efeitos dos fármacos
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 3 Ativada por Mitógeno/genética
Células-Tronco/efeitos dos fármacos
[Mh] Termos MeSH secundário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Animais
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Epitélio Anterior/lesões
Epitélio Anterior/metabolismo
Regulação da Expressão Gênica
Camundongos
Camundongos Endogâmicos BALB C
Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Técnicas de Cultura de Órgãos
Fosfoproteínas/genética
Fosfoproteínas/metabolismo
Fosforilação/efeitos dos fármacos
Complexo Repressor Polycomb 1/genética
Complexo Repressor Polycomb 1/metabolismo
Inibidores de Proteínas Quinases/farmacologia
Proteínas Proto-Oncogênicas/genética
Proteínas Proto-Oncogênicas/metabolismo
Células-Tronco/metabolismo
Células-Tronco/patologia
Transativadores/genética
Transativadores/metabolismo
Cicatrização/efeitos dos fármacos
Cicatrização/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (Abcg2 protein, mouse); 0 (Betacellulin); 0 (Bmi1 protein, mouse); 0 (Btc protein, mouse); 0 (Phosphoproteins); 0 (Protein Kinase Inhibitors); 0 (Proto-Oncogene Proteins); 0 (Trans-Activators); 0 (Trp63 protein, mouse); EC 2.3.2.27 (Polycomb Repressive Complex 1); EC 2.7.11.24 (Mapk1 protein, mouse); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


  4 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28468172
[Au] Autor:Heo KW; Park SK; Lee YM; Choe SH; Gu PM; Hong TU; Hur DY
[Ad] Endereço:*Department of Otorhinolaryngology-Head and Neck Surgery †Department of Anatomy and Research Center for Tumor Immunology, Inje University Busan Paik Hospital, Busan, South Korea.
[Ti] Título:Methotrexate Induces Apoptosis in Organ-Cultured Nasal Polyps Via the Fas Pathway.
[So] Source:J Craniofac Surg;28(3):806-809, 2017 May.
[Is] ISSN:1536-3732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Methotrexate (MTX) is very effective when used to treat chronic inflammatory diseases, and also induces apoptosis in nasal polyps (NPs). Increasing evidence suggests that Fas-Fas ligand (FasL) interactions activate multiple pathways involved in the regulation of immune and inflammatory cell functions. The aim of the present study was to identify pathways activated by Fas signaling when NPs were treated with MTX. METHODS: Nasal polyps tissues were cultured using an air-liquid interface organ culture method. Cultures were maintained in the absence or presence of MTX (10 or 100 µM) for 24 hours. The authors used the reverse transcription-polymerase chain reaction method and Western blotting to identify pathways activated by Fas when NPs were treated with MTX. RESULTS: The Fas mRNA expression ratio was unchanged upon MTX treatment, but the FasL mRNA expression ratio was significantly higher in MTX-treated than nontreated polyps. In addition, the expression levels of the Fas and FasL proteins were significantly higher in polyps treated with both 10 and 100 µM MTX compared with nontreated polyps. CONCLUSIONS: Methotrexate induces apoptosis in NPs via the Fas pathway. Future studies should explore the topical use of MTX for NP control. Methotrexate may be a useful alternative steroid-sparing agent for the treatment of NPs.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Proteína Ligante Fas/genética
Regulação da Expressão Gênica
Metotrexato/farmacologia
Pólipos Nasais/patologia
Técnicas de Cultura de Órgãos/métodos
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Apoptose/genética
Western Blotting
Proteína Ligante Fas/biossíntese
Seres Humanos
Imunossupressores/uso terapêutico
Pólipos Nasais/tratamento farmacológico
Pólipos Nasais/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FASLG protein, human); 0 (Fas Ligand Protein); 0 (Immunosuppressive Agents); 0 (RNA, Messenger); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1097/SCS.0000000000003562


  5 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29326255
[Au] Autor:Shultz D
[Ti] Título:Creating a modern monster.
[So] Source:Science;359(6372):151, 2018 Jan 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Órgãos Artificiais
Biônica
Clonagem de Organismos
Técnicas de Cultura de Órgãos
Transplantes
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1126/science.359.6372.151


  6 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28471698
[Au] Autor:Akintewe OO; Roberts EG; Rim NG; Ferguson MAH; Wong JY
[Ad] Endereço:Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215; email: olukemi@bu.edu , ngrim@bu.edu , fergu@bu.edu.
[Ti] Título:Design Approaches to Myocardial and Vascular Tissue Engineering.
[So] Source:Annu Rev Biomed Eng;19:389-414, 2017 Jun 21.
[Is] ISSN:1545-4274
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Engineered tissues represent an increasingly promising therapeutic approach for correcting structural defects and promoting tissue regeneration in cardiovascular diseases. One of the challenges associated with this approach has been the necessity for the replacement tissue to promote sufficient vascularization to maintain functionality after implantation. This review highlights a number of promising prevascularization design approaches for introducing vasculature into engineered tissues. Although we focus on encouraging blood vessel formation within myocardial implants, we also discuss techniques developed for other tissues that could eventually become relevant to engineered cardiac tissues. Because the ultimate solution to engineered tissue vascularization will require collaboration between wide-ranging disciplines such as developmental biology, tissue engineering, and computational modeling, we explore contributions from each field.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Vasos Sanguíneos/crescimento & desenvolvimento
Coração/crescimento & desenvolvimento
Dispositivos Lab-On-A-Chip
Técnicas de Cultura de Órgãos/métodos
Engenharia Tecidual/métodos
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Reatores Biológicos
Vasos Sanguíneos/citologia
Células Cultivadas
Seres Humanos
Miocárdio/citologia
Técnicas de Cultura de Órgãos/instrumentação
Engenharia Tecidual/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-bioeng-071516-044641


  7 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470123
[Au] Autor:Dojo K; Yamaguchi Y; Fustin JM; Doi M; Kobayashi M; Okamura H
[Ad] Endereço:Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
[Ti] Título:Carbachol Induces Phase-dependent Phase Shifts of Per1 Transcription Rhythms in Cultured Suprachiasmatic Nucleus Slices.
[So] Source:J Biol Rhythms;32(2):101-108, 2017 Apr.
[Is] ISSN:1552-4531
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among nonphotic stimulants, a classic cholinergic agonist, carbachol, is known to have a strong and unique phase-resetting effect on the circadian clock: Intracerebroventricular carbachol treatment causes phase delays during the subjective early night and phase advances in the subjective late night, but the effects of this drug on the suprachiasmatic nucleus (SCN) in vivo and in vitro are still controversial. In the present study, we succeeded in reproducing the biphasic phase-shifting effect of carbachol on clock gene expression in organotypic SCN slices prepared from mice carrying a Per1-promoter fused luciferase gene ( Per1-luc). Since this biphasic effect of carbachol in Per1-luc SCN was prevented by atropine but not by mecamylamine, we concluded that these phase shifts were muscarinic receptor-dependent. Next, we analyzed the expression of muscarinic receptors in the SCN by in situ hybridization and found that M3 and M4 subtypes were expressed in SCN cells. These signals appeared neonatally and reached adult levels at postnatal day 10. Together, these findings suggest that carbachol has a phase-dependent phase-shifting effect on the SCN clock through muscarinic receptor subtypes expressed in the SCN.
[Mh] Termos MeSH primário: Carbacol/farmacologia
Agonistas Colinérgicos/farmacologia
Ritmo Circadiano/efeitos dos fármacos
Proteínas Circadianas Period/genética
Núcleo Supraquiasmático/efeitos dos fármacos
Núcleo Supraquiasmático/fisiologia
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Atropina/farmacologia
Relógios Circadianos/efeitos dos fármacos
Expressão Gênica
Luciferases/genética
Mecamilamina/farmacologia
Camundongos
Atividade Motora
Antagonistas Muscarínicos/farmacologia
Antagonistas Nicotínicos/farmacologia
Técnicas de Cultura de Órgãos
Regiões Promotoras Genéticas
Receptores Muscarínicos/genética
Receptores Muscarínicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Agonists); 0 (Muscarinic Antagonists); 0 (Nicotinic Antagonists); 0 (Per1 protein, mouse); 0 (Period Circadian Proteins); 0 (Receptors, Muscarinic); 6EE945D3OK (Mecamylamine); 7C0697DR9I (Atropine); 8Y164V895Y (Carbachol); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1177/0748730417691205


  8 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27771052
[Au] Autor:Zhao W; Alama T; Kusamori K; Katsumi H; Sakane T; Yamamoto A
[Ad] Endereço:Department of Biopharmaceutics, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
[Ti] Título:Effects of 2 Polyoxyethylene Alkyl Ethers on the Function of Intestinal P-glycoprotein and Their Inhibitory Mechanisms.
[So] Source:J Pharm Sci;105(12):3668-3679, 2016 Dec.
[Is] ISSN:1520-6017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to investigate the effects of polyoxyethylene 10-oleyl ether and polyoxyethylene 9-lauryl ether, 2 polyoxyethylene alkyl ethers, on the transport and absorption of 2 P-glycoprotein (P-gp) substrates, quinidine and prednisolone, across the intestinal membrane and to elucidate the inhibitory mechanisms of intestinal P-gp by these polyoxyethylene alkyl ethers. For in vitro studies, we used a diffusion chamber method and the Caco-2 cell model. An in situ closed-loop method was used for in vivo study. The 2 polyoxyethylene alkyl ethers, nonionic surfactants, increased the intestinal absorptive transport of quinidine and prednisolone in the diffusion chamber studies, and absorptive permeability was enhanced in the in vitro Caco-2 cell study. Furthermore, these surfactants enhanced the rat intestinal absorption of prednisolone, and we observed no intestinal membrane damage in the presence of these surfactants. Furthermore, these surfactants increased membrane fluidity in intestinal brush border membranes and inhibited P-gp ATPase activity. For in vitro and in vivo studies, these surfactants enhanced the intestinal absorption of quinidine and prednisolone, 2 P-gp substrates. The alteration in intestinal membrane fluidity and the inhibition of P-gp ATPase activity by these 2 polyoxyethylene alkyl ethers may be confirmed as mechanisms of P-gp inhibition.
[Mh] Termos MeSH primário: Subfamília B de Transportador de Cassetes de Ligação de ATP/antagonistas & inibidores
Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo
Absorção Intestinal/fisiologia
Polietilenoglicóis/metabolismo
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Seres Humanos
Absorção Intestinal/efeitos dos fármacos
Masculino
Técnicas de Cultura de Órgãos
Polietilenoglicóis/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (polyoxyethylene-10-oleyl ether); 0AWH8BFG9A (polidocanol); 30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  9 / 24310 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29040332
[Au] Autor:Fukuzawa R; Anaka MR; Morison IM; Reeve AE
[Ad] Endereço:Cancer Genetics Laboratory, Department of Biochemistry, University of Otago, Dunedin, New Zealand.
[Ti] Título:The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour.
[So] Source:PLoS One;12(10):e0186333, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wilms tumour (WT) is an embryonal tumour that recapitulates kidney development. The normal kidney is formed from two distinct embryological origins: the metanephric mesenchyme (MM) and the ureteric bud (UB). It is generally accepted that WT arises from precursor cells in the MM; however whether UB-equivalent structures participate in tumorigenesis is uncertain. To address the question of the involvement of UB, we assessed 55 Wilms tumours for the molecular features of MM and UB using gene expression profiling, immunohistochemsitry and immunofluorescence. Expression profiling primarily based on the Genitourinary Molecular Anatomy Project data identified molecular signatures of the UB and collecting duct as well as those of the proximal and distal tubules in the triphasic histology group. We performed immunolabeling for fetal kidneys and WTs. We focused on a central epithelial blastema pattern which is the characteristic of triphasic histology characterized by UB-like epithelial structures surrounded by MM and MM-derived epithelial structures, evoking the induction/aggregation phase of the developing kidney. The UB-like epithelial structures and surrounding MM and epithelial structures resembling early glomerular epithelium, proximal and distal tubules showed similar expression patterns to those of the developing kidney. These observations indicate WTs can arise from a precursor cell capable of generating the entire kidney, such as the cells of the intermediate mesoderm from which both the MM and UB are derived. Moreover, this provides an explanation for the variable histological features of mesenchymal to epithelial differentiation seen in WT.
[Mh] Termos MeSH primário: Rim/metabolismo
Mesoderma/metabolismo
Ureter/metabolismo
Tumor de Wilms/genética
[Mh] Termos MeSH secundário: Carcinogênese/genética
Diferenciação Celular/genética
Membrana Celular/genética
Membrana Celular/metabolismo
Feto/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Rim/crescimento & desenvolvimento
Rim/patologia
Mesoderma/crescimento & desenvolvimento
Técnicas de Cultura de Órgãos
Organogênese/genética
Ureter/crescimento & desenvolvimento
Tumor de Wilms/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186333


  10 / 24310 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29024668
[Au] Autor:Sheffield MEJ; Adoff MD; Dombeck DA
[Ad] Endereço:Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.
[Ti] Título:Increased Prevalence of Calcium Transients across the Dendritic Arbor during Place Field Formation.
[So] Source:Neuron;96(2):490-504.e5, 2017 Oct 11.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hippocampal place cell ensembles form a cognitive map of space during exposure to novel environments. However, surprisingly little evidence exists to support the idea that synaptic plasticity in place cells is involved in forming new place fields. Here we used high-resolution functional imaging to determine the signaling patterns in CA1 soma, dendrites, and axons associated with place field formation when mice are exposed to novel virtual environments. We found that putative local dendritic spikes often occur prior to somatic place field firing. Subsequently, the first occurrence of somatic place field firing was associated with widespread regenerative dendritic events, which decreased in prevalence with increased novel environment experience. This transient increase in regenerative events was likely facilitated by a reduction in dendritic inhibition. Since regenerative dendritic events can provide the depolarization necessary for Hebbian potentiation, these results suggest that activity-dependent synaptic plasticity underlies the formation of many CA1 place fields.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Região CA1 Hipocampal/metabolismo
Cálcio/metabolismo
Dendritos/metabolismo
Locomoção/fisiologia
Plasticidade Neuronal/fisiologia
[Mh] Termos MeSH secundário: Animais
Região CA1 Hipocampal/química
Região CA1 Hipocampal/citologia
Dendritos/química
Masculino
Camundongos
Camundongos da Linhagem 129
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Técnicas de Cultura de Órgãos
Prevalência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE



página 1 de 2431 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde