Base de dados : MEDLINE
Pesquisa : E05.522 [Categoria DeCS]
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  1 / 17781 MEDLINE  
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[PMID]:28449301
[Au] Autor:van Delft P; Akay A; Huber SM; Bueschl C; Rudolph KLM; Di Domenico T; Schuhmacher R; Miska EA; Balasubramanian S
[Ad] Endereço:Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
[Ti] Título:The Profile and Dynamics of RNA Modifications in Animals.
[So] Source:Chembiochem;18(11):979-984, 2017 06 01.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:More than a hundred distinct modified nucleosides have been identified in RNA, but little is known about their distribution across different organisms, their dynamic nature and their response to cellular and environmental stress. Mass-spectrometry-based methods have been at the forefront of identifying and quantifying modified nucleosides. However, they often require synthetic reference standards, which do not exist in the case of many modified nucleosides, and this therefore impedes their analysis. Here we use a metabolic labelling approach to achieve rapid generation of bio-isotopologues of the complete Caenorhabditis elegans transcriptome and its modifications and use them as reference standards to characterise the RNA modification profile in this multicellular organism through an untargeted liquid-chromatography tandem high-resolution mass spectrometry (LC-HRMS) approach. We furthermore show that several of these RNA modifications have a dynamic response to environmental stress and that, in particular, changes in the tRNA wobble base modification 5-methoxycarbonylmethyl-2-thiouridine (mcm s U) lead to codon-biased gene-expression changes in starved animals.
[Mh] Termos MeSH primário: Processamento Pós-Transcricional do RNA
Estresse Fisiológico/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans
Cromatografia Líquida
Marcação por Isótopo
Espectrometria de Massas em Tandem
Tiouridina/análogos & derivados
Tiouridina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5-carbomethoxymethyl-2-thiouridine); 13957-31-8 (Thiouridine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700093


  2 / 17781 MEDLINE  
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[PMID]:29381065
[Au] Autor:Lin N; Chen S; Zhang H; Li J; Fu L
[Ti] Título:Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.
[So] Source:J Agric Food Chem;66(5):1270-1278, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Major royal jelly protein 1 (MRJP1) is the most abundant protein in royal jelly (RJ), and the level of MRJP1 has been suggested as a promising parameter for standardization and evaluation of RJ authenticity in quality. Here, a quantitative method was developed for the quantification of MRJP1 in RJ based on a signature peptide and a stable isotope-labeled internal standard peptide FFDYDFGSDER*(R*, C , N ) by ultraperformance liquid chromatography-tandem mass spectrometry. Recoveries of the established method ranged from 85.33 to 95.80%, and both the intra- and interday precision were RSD < 4.97%. Quantification results showed that content of MRJP1 in fresh RJ was 41.96-55.01 mg/g. Abnormal levels of MRJP1 were found in three commercial RJs and implied that these samples were of low quality and might be adulterated. Results of the present work suggested that the developed method could be successfully applied to quantify MRJP1 in RJ and also could evaluate the quality of RJ.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Ácidos Graxos/química
Glicoproteínas/análise
Proteínas de Insetos/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Isótopos de Carbono
Marcação por Isótopo
Isótopos de Nitrogênio
Fragmentos de Peptídeos/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (Fatty Acids); 0 (Glycoproteins); 0 (Insect Proteins); 0 (MRJP1 protein, Apis mellifera); 0 (Nitrogen Isotopes); 0 (Peptide Fragments); L497I37F0C (royal jelly)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05698


  3 / 17781 MEDLINE  
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[PMID]:28470617
[Au] Autor:Sugiki T; Fujiwara T; Kojima C
[Ad] Endereço:Graduate School of Engineering, Yokohama National University, Yokohama, Japan.
[Ti] Título:Cold-Shock Expression System in E. coli for Protein NMR Studies.
[So] Source:Methods Mol Biol;1586:345-357, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies.
[Mh] Termos MeSH primário: Clonagem Molecular/métodos
Resposta ao Choque Frio
Escherichia coli/genética
Vetores Genéticos/genética
[Mh] Termos MeSH secundário: Técnicas de Cultura de Células/métodos
Temperatura Baixa
Escherichia coli/fisiologia
Marcação por Isótopo/métodos
Ressonância Magnética Nuclear Biomolecular/métodos
Proteínas Recombinantes/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6887-9_23


  4 / 17781 MEDLINE  
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[PMID]:28456973
[Au] Autor:Thelen M; Winter D; Braulke T; Gieselmann V
[Ad] Endereço:Institute for Biochemistry and Molecular Biology, Rheinische-Friedrich-Wilhelms-University, Bonn, Germany. mthelen@uni-bonn.de.
[Ti] Título:SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS.
[So] Source:Methods Mol Biol;1594:1-18, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mass spectrometry-based proteomics of lysosomal proteins has led to significant advances in understanding lysosomal function and pathology. The ever-increasing sensitivity and resolution of mass spectrometry in combination with labeling procedures which allow comparative quantitative proteomics can be applied to shed more light on the steadily increasing range of lysosomal functions. In addition, investigation of alterations in lysosomal protein composition in the many lysosomal storage diseases may yield further insights into the molecular pathology of these disorders. Here, we describe a protocol which allows to determine quantitative differences in the lysosomal proteome of cells which are genetically and/or biochemically different or have been exposed to certain stimuli. The method is based on stable isotope labeling of amino acids in cell culture (SILAC). Cells are exposed to superparamagnetic iron oxide particles which are endocytosed and delivered to lysosomes. After homogenization of cells, intact lysosomes are rapidly enriched by passing the cell homogenates over a magnetic column. Lysosomes are eluted after withdrawal of the magnetic field and subjected to mass spectrometry.
[Mh] Termos MeSH primário: Aminoácidos/química
Cromatografia Líquida/métodos
Marcação por Isótopo/métodos
Lisossomos/metabolismo
Proteômica/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6934-0_1


  5 / 17781 MEDLINE  
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[PMID]:29298299
[Au] Autor:Youngblut ND; Barnett SE; Buckley DH
[Ad] Endereço:School of Integrative Plant Science, Cornell University, Ithaca, New York, United States of America.
[Ti] Título:HTSSIP: An R package for analysis of high throughput sequencing data from nucleic acid stable isotope probing (SIP) experiments.
[So] Source:PLoS One;13(1):e0189616, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Combining high throughput sequencing with stable isotope probing (HTS-SIP) is a powerful method for mapping in situ metabolic processes to thousands of microbial taxa. However, accurately mapping metabolic processes to taxa is complex and challenging. Multiple HTS-SIP data analysis methods have been developed, including high-resolution stable isotope probing (HR-SIP), multi-window high-resolution stable isotope probing (MW-HR-SIP), quantitative stable isotope probing (qSIP), and ΔBD. Currently, there is no publicly available software designed specifically for analyzing HTS-SIP data. To address this shortfall, we have developed the HTSSIP R package, an open-source, cross-platform toolset for conducting HTS-SIP analyses in a straightforward and easily reproducible manner. The HTSSIP package, along with full documentation and examples, is available from CRAN at https://cran.r-project.org/web/packages/HTSSIP/index.html and Github at https://github.com/buckleylab/HTSSIP.
[Mh] Termos MeSH primário: Sequenciamento de Nucleotídeos em Larga Escala/métodos
[Mh] Termos MeSH secundário: Marcação por Isótopo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189616


  6 / 17781 MEDLINE  
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[PMID]:28467334
[Au] Autor:Maioli C; Luciniani G; Strinchini A; Tagliabue L; Del Sole A
[Ad] Endereço:University of Milan, Department of Health Science, Via di Rudinì 8, Milan, Italy. claudio.maioli@unimi.it.
[Ti] Título:Quality control on radiochemical purity in Technetium-99m radiopharmaceuticals labelling: three years of experience on 2280 procedures.
[So] Source:Acta Biomed;88(1):49-56, 2017 Apr 28.
[Is] ISSN:0392-4203
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: the purpose of this study was to offer an example of evaluations of the ISO9001 certified internal quality assurance (QA) system of 99mTc-radiopharmaceutical preparations and quality control in vivo use, using industrial kits and generators in order to identify possible sources of errors in the procedures labeling and quality control procedures. METHODS: The study was performed at a single institution over a period of three years (July 1st, 2011 - July 1st, 2014), and included a total of 2280 radiopharmaceutical preparations prepared by four different technologists. All the radiopharmaceutical preparations and quality controls were performed according to each SPC provided by the manufacturer. The radiopharmaceutical preparations were the following (trade names are reported in brackets): 99mTc-albumin colloid [Nanocoll] (n=349), 99mTc-oxidronate [Technescan®hdp] (n=701), 99mTc-exametazime [Ceretec] (n=169), 99mTc-sestamibi [Cardiolite] (n=92), 99mTc-albumin aggregated [Technescan®lyomaa] (n=140), 99mTc-tetrofosmin [Myoview]) (n=567), 99mTc-diethylene triamine pentacetic acid [Technescan®dtpa] (n=254), and 99mTc-dimercapto succinic acid [Renocis®] (n=8). Data were analyzed to determine the number and type of radiopharmaceutical labelling failure and to derive the sources of these failures to define corrective actions and optimize the quality assurance program. RESULTS: A total of 2280 procedures were performed and recorded. Following the quality control procedure six out of the 2280 preparations (0.26%) were non-conforming for clinical use with the RCP limits indicated in the SPC. Five of these were due to gross technical errors in measurements and manual procedures and were immediately repeated, returning within the limits of acceptability. The sixth failure was due to short incubation time, though compliant with the manufacturer's instructions. CONCLUSIONS: We concluded that the quality of the final product depends on a controlled production system based on the implementation of specific standard operating procedures (ISO9001, SOP) for each radiopharmaceutical production, according to strict adherence to the SPC of each radiopharmaceutical. Based on these conclusions, in our opinion every quality control suggesting a possible error in the synthesis procedure recommended in the SPC should be immediately reported to the manufacturer, for a revision of the SPC, as well as to the regulatory agencies for an alert. This strategy may in fact allow the continuous improvement of the labelling procedures and therefore the optimization of the quality control procedures frequency to ensure both patients safety and a more rational management of resources for economic sustainability.
[Mh] Termos MeSH primário: Marcação por Isótopo/normas
Compostos de Organotecnécio/química
Controle de Qualidade
Compostos Radiofarmacêuticos/química
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organotechnetium Compounds); 0 (Radiopharmaceuticals)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180125
[Lr] Data última revisão:
180125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.23750/abm.v88i1.5285


  7 / 17781 MEDLINE  
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[PMID]:29267346
[Au] Autor:Westrop GD; Wang L; Blackburn GJ; Zhang T; Zheng L; Watson DG; Coombs GH
[Ad] Endereço:Strathclyde Institute of Pharmacy and Biomedical Science, Strathclyde University, Glasgow, United Kingdom.
[Ti] Título:Metabolomic profiling and stable isotope labelling of Trichomonas vaginalis and Tritrichomonas foetus reveal major differences in amino acid metabolism including the production of 2-hydroxyisocaproic acid, cystathionine and S-methylcysteine.
[So] Source:PLoS One;12(12):e0189072, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trichomonas vaginalis and Tritrichomonas foetus are pathogens that parasitise, respectively, human and bovine urogenital tracts causing disease. Using LC-MS, reference metabolomic profiles were obtained for both species and stable isotope labelling with D-[U-13C6] glucose was used to analyse central carbon metabolism. This facilitated a comparison of the metabolic pathways of T. vaginalis and T. foetus, extending earlier targeted biochemical studies. 43 metabolites, whose identities were confirmed by comparison of their retention times with authentic standards, occurred at more than 3-fold difference in peak intensity between T. vaginalis and T. foetus. 18 metabolites that were removed from or released into the medium during growth also showed more than 3-fold difference between the species. Major differences were observed in cysteine and methionine metabolism in which homocysteine, produced as a bi-product of trans-methylation, is catabolised by methionine γ-lyase in T. vaginalis but converted to cystathionine in T. foetus. Both species synthesise methylthioadenosine by an unusual mechanism, but it is not used as a substrate for methionine recycling. T. vaginalis also produces and exports high levels of S-methylcysteine, whereas only negligible levels were found in T. foetus which maintains significantly higher intracellular levels of cysteine. 13C-labeling confirmed that both cysteine and S-methylcysteine are synthesised by T. vaginalis; S-methylcysteine can be generated by recombinant T. vaginalis cysteine synthase using phosphoserine and methanethiol. T. foetus contained higher levels of ornithine and citrulline than T. vaginalis and exported increased levels of putrescine, suggesting greater flux through the arginine dihydrolase pathway. T. vaginalis produced and exported hydroxy acid derivatives of certain amino acids, particularly 2-hydroxyisocaproic acid derived from leucine, whereas negligible levels of these metabolites occurred in T. foetus.
[Mh] Termos MeSH primário: Aminoácidos/metabolismo
Caproatos/metabolismo
Cistationina/biossíntese
Cisteína/análogos & derivados
Metabolômica
Trichomonas vaginalis/metabolismo
Tritrichomonas foetus/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Cromatografia Líquida
Cisteína/biossíntese
Glicólise
Seres Humanos
Marcação por Isótopo
Espectrometria de Massas
Trichomonas vaginalis/genética
Tritrichomonas foetus/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Caproates); 375YFJ481O (Cystathionine); 498-36-2 (alpha-hydroxyisocaproic acid); A34I1H07YM (S-methylcysteine); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189072


  8 / 17781 MEDLINE  
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[PMID]:27773674
[Au] Autor:Rafiee MR; Girardot C; Sigismondo G; Krijgsveld J
[Ad] Endereço:German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, 69120 Heidelberg, Germany; Excellence Cluster CellNetworks, Heidelberg University, 69120 Heidelberg, Germany; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
[Ti] Título:Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.
[So] Source:Mol Cell;64(3):624-635, 2016 Nov 03.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. ChIP-SICAP in mouse embryonic stem cells (ESCs) identified over 400 proteins associating with OSN, including several whose interaction depends on the pluripotent state. Trim24, a previously unrecognized protein in the network, converges with OSN on multiple enhancers and suppresses the expression of developmental genes while activating cell cycle genes. Consistently, Trim24 significantly improved efficiency of cellular reprogramming, demonstrating its direct functionality in establishing pluripotency. Collectively, ChIP-SICAP provides a powerful tool to decode chromatin protein composition, further enhanced by its integrative capacity to perform ChIP-seq.
[Mh] Termos MeSH primário: Cromatina/química
Células-Tronco Embrionárias Murinas/metabolismo
Proteína Homeobox Nanog/genética
Proteínas Nucleares/genética
Fator 3 de Transcrição de Octâmero/genética
Células-Tronco Pluripotentes/metabolismo
Fatores de Transcrição SOXB1/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Diferenciação Celular
Reprogramação Celular
Cromatina/metabolismo
Imunoprecipitação da Cromatina/métodos
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Marcação por Isótopo
Espectrometria de Massas/métodos
Camundongos
Células-Tronco Embrionárias Murinas/citologia
Proteína Homeobox Nanog/metabolismo
Proteínas Nucleares/metabolismo
Fator 3 de Transcrição de Octâmero/metabolismo
Células-Tronco Pluripotentes/citologia
Ligação Proteica
Fatores de Transcrição SOXB1/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Nanog Homeobox Protein); 0 (Nanog protein, mouse); 0 (Nuclear Proteins); 0 (Octamer Transcription Factor-3); 0 (Pou5f1 protein, mouse); 0 (SOXB1 Transcription Factors); 0 (Sox2 protein, mouse); 0 (Transcription Factors); 0 (transcriptional intermediary factor 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180114
[Lr] Data última revisão:
180114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


  9 / 17781 MEDLINE  
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[PMID]:29261795
[Au] Autor:Rezek RJ; Lebreton B; Sterba-Boatwright B; Beseres Pollack J
[Ad] Endereço:Department of Life Sciences, Texas A&M University - Corpus Christi, Corpus Christi, Texas, United States of America.
[Ti] Título:Ecological structure and function in a restored versus natural salt marsh.
[So] Source:PLoS One;12(12):e0189871, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Habitat reconstruction is commonly employed to restore degraded estuarine habitats and lost ecological functions. In this study, we use a combination of stable isotope analyses and macrofauna community analysis to compare the ecological structure and function between a recently constructed Spartina alterniflora salt marsh and a natural reference habitat over a 2-year period. The restored marsh was successful in providing habitat for economically and ecologically important macrofauna taxa; supporting similar or greater density, biomass, and species richness to the natural reference during all but one sampling period. Stable isotope analyses revealed that communities from the natural and the restored marshes relied on a similar diversity of food resources and that decapods had similar trophic levels. However, some generalist consumers (Palaemonetes spp. and Penaeus aztecus) were more 13C-enriched in the natural marsh, indicating a greater use of macrophyte derived organic matter relative to restored marsh counterparts. This difference was attributed to the higher quantities of macrophyte detritus and organic carbon in natural marsh sediments. Reduced marsh flooding frequency was associated with a reduction in macrofaunal biomass and decapod trophic levels. The restored marsh edge occurred at lower elevations than natural marsh edge, apparently due to reduced fetch and wind-wave exposure provided by the protective berm structures. The lower elevation of the restored marsh edge mitigated negative impacts in sampling periods with low tidal elevations that affected the natural marsh. The results of this study highlight the importance of considering sediment characteristics and elevation in salt marsh constructions.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Ecossistema
Salinidade
Zonas Úmidas
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Biodiversidade
Biomassa
Decápodes (Crustáceos)
Inundações
Geografia
Sedimentos Geológicos/química
Marcação por Isótopo
Modelos Teóricos
Análise Multivariada
Texas
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189871


  10 / 17781 MEDLINE  
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[PMID]:29191024
[Au] Autor:Billaud EMF; Belderbos S; Cleeren F; Maes W; Van de Wouwer M; Koole M; Verbruggen A; Himmelreich U; Geukens N; Bormans G
[Ad] Endereço:Laboratory for Radiopharmaceutical Research, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven , Campus Gasthuisberg, O&N2, Herestraat 49, Box 821, 3000 Leuven, Belgium.
[Ti] Título:Pretargeted PET Imaging Using a Bioorthogonal F-Labeled trans-Cyclooctene in an Ovarian Carcinoma Model.
[So] Source:Bioconjug Chem;28(12):2915-2920, 2017 Dec 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In cancer research, pretargeted positron emission tomography (PET) imaging has emerged as an effective two-step approach that combines the excellent target affinity and selectivity of antibodies with the advantages of using short-lived radionuclides such as fluorine-18. One possible approach is based on the bioorthogonal inverse-electron-demand Diels-Alder (IEDDA) reaction between tetrazines and trans-cyclooctene (TCO) derivatives. Here, we report the first successful use of an F-labeled small TCO compound, [ F]1 recently developed in our laboratory, to perform pretargeted immuno-PET imaging. The study was performed in an ovarian carcinoma mouse model, using a trastuzumab-tetrazine conjugate.
[Mh] Termos MeSH primário: Ciclo-Octanos/química
Radioisótopos de Flúor
Neoplasias Ovarianas/patologia
Tomografia por Emissão de Pósitrons/métodos
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Reação de Cicloadição
Feminino
Seres Humanos
Imunoconjugados/química
Imunoconjugados/farmacocinética
Marcação por Isótopo
Camundongos
Neoplasias Ovarianas/diagnóstico por imagem
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclooctanes); 0 (Fluorine Radioisotopes); 0 (Fluorine-18); 0 (Immunoconjugates)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00635



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