Base de dados : MEDLINE
Pesquisa : E05.588.570 [Categoria DeCS]
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  1 / 10099 MEDLINE  
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[PMID]:29287883
[Au] Autor:Ma Y; Shi L; Zheng C
[Ad] Endereço:Department of Otolaryngology-Head and Neck Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai Key Clinical Disciplines of Otorhinolaryngology, PR China.
[Ti] Título:Microarray analysis of lncRNA and mRNA expression profiles in mice with allergic rhinitis.
[So] Source:Int J Pediatr Otorhinolaryngol;104:58-65, 2018 Jan.
[Is] ISSN:1872-8464
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: We aimed to identify the effect of lncRNAs in CD4 T cells on Allergic rhinitis (AR). METHODS: The present study conducted a microarray to identify the expression profiles of lncRNA and mRNA in CD4 T cells in both AR murine models and normal controls. And qRT-PCR was used to confirm the results. GO and KEGG enrichment analysis were used to show all related pathways and a co-expression network was conducted to find lncRNAs which have high correlation with these pathways. RESULTS: The results showed that the two groups contained a total of 158 deregulated lncRNAs, of which 110 were upregulated and 48 were downregulated. And positive regulation of calcium ion transport, B cell activation, chemokine-signaling pathways and calcium-signaling pathways may be involved in the development of T cells in AR pathology. Finally, we can find the differentially expressed mRNA in the pathways related to T cell differentiation correlated with many deregulated lncRNAs. CONCLUSIONS: The present study was the first to show the differential expression profiles of lncRNAs in the CD4 T cells of an AR murine model, which may provide significant insights into AR pathogenesis and offer new treatment targets to alleviate it.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/metabolismo
Análise em Microsséries/métodos
RNA Longo não Codificante/metabolismo
RNA Mensageiro/metabolismo
Rinite Alérgica/genética
[Mh] Termos MeSH secundário: Animais
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase em Tempo Real
Rinite Alérgica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Long Noncoding); 0 (RNA, Messenger)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


  2 / 10099 MEDLINE  
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[PMID]:29177276
[Au] Autor:Dallabernardina P; Ruprecht C; Smith PJ; Hahn MG; Urbanowicz BR; Pfrengle F
[Ad] Endereço:Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany. Fabian.Pfrengle@mpikg.mpg.de.
[Ti] Título:Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies.
[So] Source:Org Biomol Chem;15(47):9996-10000, 2017 Dec 06.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).
[Mh] Termos MeSH primário: Anticorpos Monoclonais/química
Arabidopsis/química
Automação
Parede Celular/química
Oligossacarídeos/síntese química
Polissacarídeos/química
[Mh] Termos MeSH secundário: Arabidopsis/enzimologia
Parede Celular/enzimologia
Análise em Microsséries
Oligossacarídeos/química
Oligossacarídeos/metabolismo
Pentosiltransferases/metabolismo
Polissacarídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Oligosaccharides); 0 (Polysaccharides); EC 2.4.2.- (Pentosyltransferases); EC 2.4.2.- (xyloglucan xylosyltransferase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob02605f


  3 / 10099 MEDLINE  
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[PMID]:29480850
[Au] Autor:Cho HY; Cho Y; Shin YJ; Park J; Shim S; Jung Y; Shim S; Cha D
[Ad] Endereço:Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam.
[Ti] Título:Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction.
[So] Source:Medicine (Baltimore);97(2):e9572, 2018 Jan.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The prediction and monitoring of fetal growth restriction (FGR) fetuses has become with the use of ultrasound. However, these tools lack the fundamental evidence for the growth of fetus with FGR excluding pathogenic factors.Amniotic fluid samples were obtained from pregnant women for fetal karyotyping and genetic diagnosis at 16 to 19 weeks of gestation. For this study, 15 FGR and 9 control samples were selected, and cell-free fetal RNA was isolated from each supernatant of the amniotic fluid for microarray analysis.In this study, 411 genes were differentially expressed between the FGR and control group. Of these genes, 316 genes were up-regulated, while 95 genes were down-regulated. In terms of gene ontology, the up-regulated genes were highly related to metabolic process as well as protein synthesis, while the down-regulated genes were related to receptor activity and biological adhesion. In terms of tissue-specific expression, the up-regulated genes were involved in various organs while down-regulated genes were involved only in the brain. In terms of organ-specific expression, many genes were enriched for B-cell lymphoma, pancreas, eye, placenta, epithelium, skin, and muscle. In the functional significance of gene, low-density lipoprotein receptor-related protein 10 (LRP10) was significantly increased (6-fold) and insulin-like growth factor (IGF-2) was dramatically increased (17-fold) in the FGR cases.The results show that the important brain-related genes are predominantly down-regulated in the intrauterine growth restriction fetuses during the second trimester of pregnancy. This study also suggested possible genes related to fetal development such as B-cell lymphoma, LRP10, and IGF-2. To monitor the fetal development, further study may be needed to elucidate the role of the genes identified.
[Mh] Termos MeSH primário: Líquido Amniótico/química
Retardo do Crescimento Fetal/diagnóstico
RNA/análise
[Mh] Termos MeSH secundário: Adulto
Líquido Amniótico/metabolismo
Feminino
Retardo do Crescimento Fetal/metabolismo
Seres Humanos
Masculino
Análise em Microsséries
Gravidez
Segundo Trimestre da Gravidez
Cuidado Pré-Natal
Estudos Prospectivos
RNA/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180227
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009572


  4 / 10099 MEDLINE  
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[PMID]:28454701
[Au] Autor:Gunel T; Hosseini MK; Gumusoglu E; Kisakesen HI; Benian A; Aydinli K
[Ad] Endereço:Istanbul University, Faculty of Science, Department of Molecular Biology and Genetics, Istanbul, Turkey. Electronic address: gunel@istanbul.edu.tr.
[Ti] Título:Expression profiling of maternal plasma and placenta microRNAs in preeclamptic pregnancies by microarray technology.
[So] Source:Placenta;52:77-85, 2017 Apr.
[Is] ISSN:1532-3102
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Preeclampsia (PE) is one of the leading causes of maternal and fetal morbidity and mortality, occurring usually in the second half of pregnancy and affecting approximately 5-8% of pregnancies in the world. miRNAs play critical role in the regulation of placental development processes. We aimed to determine specific novel miRNAs for early diagnosis of preeclampsia which is one of the most dangerous pregnancy diseases. In this study 72 samples, maternal age 22 ≤ and ≤36, have been analyzed; maternal plasma and placental miRNAs were isolated from 18 severe preeclampsia (sPE) patients and 18 controls, respectively. Profiling of human miRNAs (1368 probe) was performed in samples with Agilent v16 microarrays for detection of the differences in miRNA expression between two groups. The results were validated by using TaqMan RT-qPCR method. The analysis indicated that 406 of these miRNAs in all placentas and 42 of these miRNAs in all maternal plasma were expressed. The relative expression analysis has shown that 12 miRNAs (p < 0.05 and >2-fold) in maternal plasma were differentially expressed in PE and control group. However, five miRNAs were validated by qRT-PCR. Once validated miRNAs have been searched in databases for their target genes and function, it has been shown that there are some preeclampsia related pathways as a target such as angiogenesis, cardiovascular, hypertension, placental abruption and preeclampsia disorders. Differentially expressed and validated plasma miRNAs might be used as notable biomarkers for non-invasive early diagnosis of preeclampsia and treatment of disease.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
MicroRNAs/metabolismo
Placenta/metabolismo
Pré-Eclâmpsia/metabolismo
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
MicroRNAs/sangue
MicroRNAs/genética
Análise em Microsséries
Pré-Eclâmpsia/sangue
Pré-Eclâmpsia/genética
Gravidez
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  5 / 10099 MEDLINE  
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[PMID]:29299749
[Au] Autor:Chai R; Zhang K; Wang K; Li G; Huang R; Zhao Z; Liu Y; Chen J
[Ad] Endereço:Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, No. 6 Tiantan Xili, Dongcheng District, Beijing, 100050, China.
[Ti] Título:A novel gene signature based on five glioblastoma stem-like cell relevant genes predicts the survival of primary glioblastoma.
[So] Source:J Cancer Res Clin Oncol;144(3):439-447, 2018 Mar.
[Is] ISSN:1432-1335
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Primary glioblastoma (pGBM) is the most common and lethal type of neoplasms in the central nervous system, while the existing biomarkers, lacking consideration on the stemness changes of GBM cells, are not specific enough to predict the complex prognosis respectively. We aimed to build a high-efficiency prediction gene signature related to GBM cell stemness and investigate its prognostic value in primary glioblastoma. METHODS: Differentially expressed genes were screened in GSE23806 database. The selected genes were then verified by univariate Cox regression in 591 patients from four enormous independent databases, including the Chinese Glioma Genome Atlas (CGGA), TCGA, REMBRANDT and GSE16011. Finally, the intersected genes were included to build the gene signature. GO analysis and GSEA were carried out to explore the bioinformatic implication. RESULTS: The novel five-gene signature was used to identify high- and low-risk groups in the four databases, and the high-risk group showed notably poorer prognosis (P < 0.05). Gene ontology (GO) terms including "immune response", "apoptotic process", and "angiogenesis" were picked out by GO analysis and GSEA, which revealed that the gene signature was highly possibly related to the stemness of GSCs and predicting the prognosis of GBM effectively. CONCLUSION: We built a gene signature with five glioblastoma stem-like cell (GSC) relevant genes, and predicted the survival in four independent databases effectively, which is possibly related to the stemness of GSCs in pGBM. Several GO terms were investigated to be correlated to the signature. The signature can predict the prognosis of glioblastoma efficiently.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Neoplasias Encefálicas/diagnóstico
Glioblastoma/diagnóstico
Células-Tronco Neoplásicas/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Neoplasias Encefálicas/genética
Neoplasias Encefálicas/mortalidade
Conjuntos de Dados como Assunto
Perfilação da Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Glioblastoma/genética
Glioblastoma/mortalidade
Seres Humanos
Análise em Microsséries
Valor Preditivo dos Testes
Prognóstico
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1007/s00432-017-2572-6


  6 / 10099 MEDLINE  
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[PMID]:29231925
[Au] Autor:Zhang L; Zhang X; Zhang G; Pang CP; Leung YF; Zhang M; Zhong W
[Ad] Endereço:Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA.
[Ti] Título:Expression profiling of the retina of pde6c, a zebrafish model of retinal degeneration.
[So] Source:Sci Data;4:170182, 2017 12 12.
[Is] ISSN:2052-4463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Retinal degeneration often affects the whole retina even though the disease-causing gene is specifically expressed in the light-sensitive photoreceptors. The molecular basis of the retinal defect can potentially be determined by gene-expression profiling of the whole retina. In this study, we measured the gene-expression profile of retinas microdissected from a zebrafish pde6c (pde6c) mutant. This retinal-degeneration model not only displays cone degeneration caused by a cone-specific mutation, but also other secondary cellular changes starting from 4 days postfertilization (dpf). To capture the underlying molecular changes, we subjected pde6c and wild-type (WT) retinas at 5 dpf/ 120 h postfertilization (hpf) to RNA sequencing (RNA-Seq) on the Illumina HiSeq 2,000 platform. We also validated the RNA-Seq results by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) of seven phototransduction genes. Our analyses indicate that the RNA-Seq dataset was of high quality, and effectively captured the molecular changes in the whole pde6c retina. This dataset will facilitate the characterization of the molecular defects in the pde6c retina at the initial stage of retinal degeneration.
[Mh] Termos MeSH primário: Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/genética
Retina/metabolismo
Degeneração Retiniana/genética
Proteínas de Peixe-Zebra/genética
[Mh] Termos MeSH secundário: Animais
Análise em Microsséries
Transcriptoma
Peixe-Zebra
[Pt] Tipo de publicação:DATASET; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Zebrafish Proteins); EC 3.1.4.- (Pde6c protein, zebrafish); EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 6)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1038/sdata.2017.182


  7 / 10099 MEDLINE  
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[PMID]:29384847
[Au] Autor:Lin H; Zhang Q; Li X; Wu Y; Liu Y; Hu Y
[Ad] Endereço:Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, Chongqing.
[Ti] Título:Identification of key candidate genes and pathways in hepatitis B virus-associated acute liver failure by bioinformatical analysis.
[So] Source:Medicine (Baltimore);97(5):e9687, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatitis B virus-associated acute liver failure (HBV-ALF) is a rare but life-threatening syndrome that carried a high morbidity and mortality. Our study aimed to explore the possible molecular mechanisms of HBV-ALF by means of bioinformatics analysis. In this study, genes expression microarray datasets of HBV-ALF from Gene Expression Omnibus were collected, and then we identified differentially expressed genes (DEGs) by the limma package in R. After functional enrichment analysis, we constructed the protein-protein interaction (PPI) network by the Search Tool for the Retrieval of Interacting Genes online database and weighted genes coexpression network by the WGCNA package in R. Subsequently, we picked out the hub genes among the DEGs. A total of 423 DEGs with 198 upregulated genes and 225 downregulated genes were identified between HBV-ALF and normal samples. The upregulated genes were mainly enriched in immune response, and the downregulated genes were mainly enriched in complement and coagulation cascades. Orosomucoid 1 (ORM1), orosomucoid 2 (ORM2), plasminogen (PLG), and aldehyde oxidase 1 (AOX1) were picked out as the hub genes that with a high degree in both PPI network and weighted genes coexpression network. The weighted genes coexpression network analysis found out 3 of the 5 modules that upregulated genes enriched in were closely related to immune system. The downregulated genes enriched in only one module, and the genes in this module majorly enriched in the complement and coagulation cascades pathway. In conclusion, 4 genes (ORM1, ORM2, PLG, and AOX1) with immune response and the complement and coagulation cascades pathway may take part in the pathogenesis of HBV-ALF, and these candidate genes and pathways could be therapeutic targets for HBV-ALF.
[Mh] Termos MeSH primário: Hepatite B/genética
Hepatite B/metabolismo
Falência Hepática Aguda/genética
Falência Hepática Aguda/metabolismo
[Mh] Termos MeSH secundário: Biologia Computacional
Vírus da Hepatite B
Fígado/metabolismo
Falência Hepática Aguda/etiologia
Falência Hepática Aguda/virologia
Análise em Microsséries
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009687


  8 / 10099 MEDLINE  
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[PMID]:28470525
[Au] Autor:Brown JP; Lynch BS; Curry-Chisolm IM; Shafer TJ; Strickland JD
[Ad] Endereço:Integrated Systems Toxicology Division, NHEERL, US EPA, MD105-05, Research Triangle Park, NC, 27711, USA.
[Ti] Título:Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays.
[So] Source:Methods Mol Biol;1601:153-170, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microelectrode array (MEA) technology is a neurophysiological method that allows for the spontaneous measure of activity in neural cultures and determination of drug and chemical effects thereon. Recent introduction of multi-well MEA (mwMEA) formats have dramatically increased the throughput of this technology, allowing more efficient compound screening. Rapid characterization of compounds for neuroactivity or neurotoxicity hazard evaluation following acute, chronic, or developmental exposures ideally would also consider compound effects on cell health, and to do so in the same well requires a multiplexed approach. Procedures describing the multiplexed method to acute and developmental screening are described in this chapter.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Citotoxinas/toxicidade
Análise em Microsséries/instrumentação
Microeletrodos
Rede Nervosa/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Testes de Toxicidade/instrumentação
[Mh] Termos MeSH secundário: Animais
Neocórtex/citologia
Cultura Primária de Células
Ratos
Ratos Long-Evans
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytotoxins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_13


  9 / 10099 MEDLINE  
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[PMID]:28448456
[Au] Autor:Othumpangat S; Bryan NB; Beezhold DH; Noti JD
[Ad] Endereço:Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA. seo8@cdc.gov.
[Ti] Título:Upregulation of miRNA-4776 in Influenza Virus Infected Bronchial Epithelial Cells Is Associated with Downregulation of NFKBIB and Increased Viral Survival.
[So] Source:Viruses;9(5), 2017 04 27.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Influenza A virus (IAV) infection remains a significant cause of morbidity and mortality worldwide. One key transcription factor that is activated upon IAV infection is nuclear factor Kappa B (NF-κB). NF-κB regulation involves the inhibitor proteins NF-κB inhibitor beta (NFKBIB), (also known as IκB ß), which form complexes with NF-κB to sequester it in the cytoplasm. In this study, microarray data showed differential expression of several microRNAs (miRNAs) on exposure to IAV. Target scan analysis revealed that miR-4776, miR-4514 and miR-4742 potentially target NFKBIB messenger RNA (mRNA). Time-course analysis of primary bronchial epithelial cells (HBEpCs) showed that miR-4776 expression is increased within 1 h of infection, followed by its downregulation 4 h post-exposure to IAV. NFKBIB upregulation of miR-4776 correlated with a decrease in NFKBIB expression within 1 h of infection and a subsequent increase in NFKBIB expression 4 h post-infection. In addition, miRNA ago-immunoprecipitation studies and the three prime untranslated region (3' UTR) luciferase assay confirmed that miR-4776 targets NFKBIB mRNA. Furthermore, uninfected HBEpCs transfected with miR-4776 mimic showed decreased expression of NFKBIB mRNA. Overexpression of NFKBIB protein in IAV infected cells led to lower levels of IAV. Taken together, our data suggest that miRNA-4776 modulates IAV production in infected cells through NFKBIB expression, possibly through the modulation of NF-κB.
[Mh] Termos MeSH primário: Brônquios/virologia
Células Epiteliais/virologia
Proteínas I-kappa B/genética
Vírus da Influenza A Subtipo H1N1/fisiologia
MicroRNAs/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Brônquios/citologia
Linhagem Celular
Regulação para Baixo
Regulação da Expressão Gênica
Seres Humanos
Proteínas I-kappa B/metabolismo
Análise em Microsséries
Viabilidade Microbiana
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (I kappa B beta protein); 0 (I-kappa B Proteins); 0 (MicroRNAs); 0 (RNA, Messenger)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


  10 / 10099 MEDLINE  
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[PMID]:29180488
[Au] Autor:Lin A; Liang F; Thompson EA; Vono M; Ols S; Lindgren G; Hassett K; Salter H; Ciaramella G; Loré K
[Ad] Endereço:Immunology and Allergy Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
[Ti] Título:Rhesus Macaque Myeloid-Derived Suppressor Cells Demonstrate T Cell Inhibitory Functions and Are Transiently Increased after Vaccination.
[So] Source:J Immunol;200(1):286-294, 2018 01 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myeloid-derived suppressor cells (MDSCs) are major regulators of T cell responses in several pathological conditions. Whether MDSCs increase and influence T cell responses in temporary inflammation, such as after vaccine administration, is unknown. Using the rhesus macaque model, which is critical for late-stage vaccine testing, we demonstrate that monocytic (M)-MDSCs and polymorphonuclear (PMN)-MDSCs can be detected using several of the markers used in humans. However, whereas rhesus M-MDSCs lacked expression of CD33, PMN-MDSCs were identified as CD33 low-density neutrophils. Importantly, both M-MDSCs and PMN-MDSCs showed suppression of T cell proliferation in vitro. The frequency of circulating MDSCs rapidly and transiently increased 24 h after vaccine administration. M-MDSCs infiltrated the vaccine injection site, but not vaccine-draining lymph nodes. This was accompanied by upregulation of genes relevant to MDSCs such as arginase-1, IDO1, PDL1, and IL-10 at the injection site. MDSCs may therefore play a role in locally maintaining immune balance during vaccine-induced inflammation.
[Mh] Termos MeSH primário: Vírus da Influenza A Subtipo H10N8/imunologia
Vacinas contra Influenza/imunologia
Influenza Humana/imunologia
Células Supressoras Mieloides/imunologia
Neutrófilos/imunologia
Infecções por Orthomyxoviridae/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Arginase/genética
Antígeno B7-H1/genética
Proliferação Celular
Regulação da Expressão Gênica
Seres Humanos
Tolerância Imunológica
Indolamina-Pirrol 2,3,-Dioxigenase/genética
Interleucina-10/genética
Macaca mulatta
Análise em Microsséries
Lectina 3 Semelhante a Ig de Ligação ao Ácido Siálico/metabolismo
Vacinação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (B7-H1 Antigen); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (Influenza Vaccines); 0 (Sialic Acid Binding Ig-like Lectin 3); 130068-27-8 (Interleukin-10); EC 3.5.3.1 (Arginase); EC 3.5.3.1 (arginase I, human)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1701005



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