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  1 / 5443 MEDLINE  
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[PMID]:29257652
[Au] Autor:Ganie AH; Yousuf PY; Ahad A; Pandey R; Ahmad S; Aref IM; Noor JJ; Iqbal M
[Ti] Título:Quantification of phenolic acids and antioxidant potential of inbred, hybrid and composite cultivars of maize under different nitrogen regimes.
[So] Source:J Environ Biol;37(6):1273-79, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Maize (Zea mays L.) is a multipurpose crop, which is immensely used worldwide for its nutritional as well as medicinal properties. This study evaluates the effect of varying concentrations of nitrogen (N) on accumulation of phenolic acids and antioxidant activity in different maize cultivars, including inbreds, hybrids and a composite, which were grown in natural light under controlled temperature (30°C/20°C D/N) and humidity (80%), with sufficient (4.5mM) and low (0.05mM) nitrogen supply. Seeds of different cultivars were powdered and extracted in a methanol:water (80:20) mixture through reflux at 60-75°C, and the extracts obtained were subjected to high performance thin layer chromatography (HPTLC), using ethyl acetate: acetic acid: formic acid: water (109:16:12:31) solvent system for the separation of phenolic acids. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and H2O2-scavenging activity assays. At sufficient nitrogen condition, the contents of different phenolic acids were higher in the composite cultivar (8.7 mg g-1 d.wt. in gallic acid to 39.3 mg g-1 d.wt. in cinnamic and salicylic acids) than in inbreds and hybrids. Under low nitrogen condition, the phenolic acids contents declined significantly in inbreds and hybrids, but remained almost unaffected in the composite. The antioxidant activity was also the maximum in the composite, and declined similarly as phenolic acids under low nitrogen supply, showing a significant reduction in inbreds and hybrids only. Therefore, the maize composite has a potential for being used as a nutraceutical in human-health sector.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Hidroxibenzoatos/metabolismo
Nitrogênio/farmacologia
Zea mays/genética
Zea mays/fisiologia
[Mh] Termos MeSH secundário: Antioxidantes/química
Cruzamentos Genéticos
Hidroxibenzoatos/química
Endogamia
Zea mays/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Hydroxybenzoates); 29656-58-4 (phenolic acid); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  2 / 5443 MEDLINE  
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[PMID]:28748554
[Au] Autor:Martikainen K; Tyrisevä AM; Matilainen K; Pösö J; Uimari P
[Ad] Endereço:Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
[Ti] Título:Estimation of inbreeding depression on female fertility in the Finnish Ayrshire population.
[So] Source:J Anim Breed Genet;134(5):383-392, 2017 Oct.
[Is] ISSN:1439-0388
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Single nucleotide polymorphism (SNP) data enable the estimation of inbreeding at the genome level. In this study, we estimated inbreeding levels for 19,075 Finnish Ayrshire cows genotyped with a low-density SNP panel (8K). The genotypes were imputed to 50K density, and after quality control, 39,144 SNPs remained for the analysis. Inbreeding coefficients were estimated for each animal based on the percentage of homozygous SNPs (F ), runs of homozygosity (F ) and pedigree (F ). Phenotypic records were available for 13,712 animals including non-return rate (NRR), number of inseminations (AIS) and interval from first to last insemination (IFL) for heifers and up to three parities for cows, as well as interval from calving to first insemination (ICF) for cows. Average F was 0.02, F 0.06 and F 0.63. A correlation of 0.71 was found between F and F , 0.66 between F and F and 0.94 between F and F . Pedigree-based inbreeding coefficients did not show inbreeding depression in any of the traits. However, when F or F was used as a covariate, significant inbreeding depression was observed; a 10% increase in F was associated with 5 days longer IFL0 and IFL1, 2 weeks longer IFL3 and 3 days longer ICF2 compared to non-inbred cows.
[Mh] Termos MeSH primário: Bovinos/genética
Bovinos/fisiologia
Fertilidade
Depressão por Endogamia
Polimorfismo de Nucleotídeo Único
Característica Quantitativa Herdável
[Mh] Termos MeSH secundário: Animais
Feminino
Finlândia
Genômica
Endogamia
Fenótipo
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1111/jbg.12285


  3 / 5443 MEDLINE  
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[PMID]:29261806
[Au] Autor:Zubrzycki JE; Maringolo CA; Filippi CV; Quiróz FJ; Nishinakamasu V; Puebla AF; Di Rienzo JA; Escande A; Lia VV; Heinz RA; Hopp HE; Cervigni GDL; Paniego NB
[Ad] Endereço:Instituto de Biotecnología, Centro de Investigaciones en Ciencias Agronómicas y Veterinarias, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina.
[Ti] Título:Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower.
[So] Source:PLoS One;12(12):e0189859, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant Inbred Lines (RILs) along with their parental lines (PAC2 -partially resistant-and RHA266 -susceptible-) by using a 384 single nucleotide polymorphism (SNP) Illumina Oligo Pool Assay to saturate a sunflower genetic map. Subsequently, we tested these lines for SHR resistance using assisted inoculations with S. sclerotiorum ascospores. We also conducted a randomized complete-block assays with three replicates to visually score disease incidence (DI), disease severity (DS), disease intensity (DInt) and incubation period (IP) through four field trials (2010-2014). We finally assessed main effect quantitative trait loci (M-QTLs) and epistatic QTLs (E-QTLs) by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively. As a result of this study, the improved map incorporates 61 new SNPs over candidate genes. We detected a broad range of narrow sense heritability (h2) values (1.86-59.9%) as well as 36 M-QTLs and 13 E-QTLs along 14 linkage groups (LGs). On LG1, LG10, and LG15, we repeatedly detected QTLs across field trials; which emphasizes their putative effectiveness against SHR. In all selected variables, most of the identified QTLs showed high determination coefficients, associated with moderate to high heritability values. Using markers shared with previous Sclerotinia resistance studies, we compared the QTL locations in LG1, LG2, LG8, LG10, LG11, LG15 and LG16. This study constitutes the largest report of QTLs for SHR resistance in sunflower. Further studies focusing on the regions in LG1, LG10, and LG15 harboring the detected QTLs are necessary to identify causal alleles and contribute to unraveling the complex genetic basis governing the resistance.
[Mh] Termos MeSH primário: Ascomicetos/fisiologia
Resistência à Doença/genética
Epistasia Genética
Helianthus/genética
Helianthus/microbiologia
Doenças das Plantas/microbiologia
Locos de Características Quantitativas/genética
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Ligação Genética
Marcadores Genéticos
Genótipo
Endogamia
Fenótipo
Polimorfismo de Nucleotídeo Único/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189859


  4 / 5443 MEDLINE  
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[PMID]:29261718
[Au] Autor:Bhusal N; Sarial AK; Sharma P; Sareen S
[Ad] Endereço:Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, Haryana, India.
[Ti] Título:Mapping QTLs for grain yield components in wheat under heat stress.
[So] Source:PLoS One;12(12):e0189594, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The current perspective of increasing global temperature makes heat stress as a major threat to wheat production worldwide. In order to identify quantitative trait loci (QTLs) associated with heat tolerance, 251 recombinant inbred lines (RILs) derived from a cross between HD2808 (heat tolerant) and HUW510 (heat susceptible) were evaluated under timely sown (normal) and late sown (heat stress) conditions for two consecutive crop seasons; 2013-14 and 2014-15. Grain yield (GY) and its components namely, grain weight/spike (GWS), grain number/spike (GNS), thousand grain weight (TGW), grain filling rate (GFR) and grain filling duration (GFD) were recorded for both conditions and years. The data collected for both timely and late sown conditions and heat susceptibility index (HSI) of these traits were used as phenotypic data for QTL identification. The frequency distribution of HSI for all the studied traits was continuous during both the years and also included transgressive segregants. Composite interval mapping identified total 24 QTLs viz., 9 (timely sown traits), 6 (late sown traits) and 9 (HSI of traits) mapped on linkage groups 2A, 2B, and 6D during both the crop seasons 2013-14 and 2014-15. The QTLs were detected for GWS (6), GNS (6), GFR (4), TGW (3), GY (3) and GFD (2). The LOD score of identified QTLs varied from 3.03 (Qtgns.iiwbr-6D) to 21.01 (Qhsitgw.iiwbr-2A) during 2014-15, explaining 11.2 and 30.6% phenotypic variance, respectively. Maximum no of QTLs were detected in chromosome 2A followed by 6D and 2B. All the QTL detected under late sown and HSI traits were identified on chromosome 2A except for QTLs associated with GFD. Fifteen out of 17 QTL detected on chromosome 2A were clustered within the marker interval between gwm448 and wmc296 and showed tight linkage with gwm122 and these were localized in 49-52 cM region of Somers consensus map of chromosome 2A i.e. within 18-59.56 cM region of chromosome 2A where no QTL related to heat stress were reported earlier. Besides, three consistent QTLs, Qgws.iiwbr-2A, Qgns.iiwbr-2A and Qgns.iiwbr-2A were also detected in all the environments in this region. The nearest QTL detected in earlier studies, QFv/Fm.cgb-2A was approximately 6cM below the presently identified QTLs region, respectively Additionally, QTLs for physiological and phenological traits and plant height under late sown and HSI of these traits were also detected on chromosome 2A. QTL for HSI of plant height and physiological maturity were located in the same genomic region of chromosome 2Awhereas QTLs for physiological and phonological traits under late sown were located 8cM and 33.5 cM below the genomic location associated with grain traits, respectively in consensus map of Somers. This QTL hot-spot region with consistent QTLs could be used to improve heat tolerance after validation.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Grãos Comestíveis/genética
Temperatura Alta
Locos de Características Quantitativas/genética
Estresse Fisiológico/genética
Triticum/genética
Triticum/fisiologia
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Cromossomos de Plantas/genética
Produtos Agrícolas/genética
Produtos Agrícolas/fisiologia
Epistasia Genética
Endogamia
Fenótipo
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189594


  5 / 5443 MEDLINE  
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[PMID]:28463982
[Au] Autor:Purfield DC; McParland S; Wall E; Berry DP
[Ad] Endereço:Animal & Grassland Research and Innovation Center, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.
[Ti] Título:The distribution of runs of homozygosity and selection signatures in six commercial meat sheep breeds.
[So] Source:PLoS One;12(5):e0176780, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Domestication and the subsequent selection of animals for either economic or morphological features can leave a variety of imprints on the genome of a population. Genomic regions subjected to high selective pressures often show reduced genetic diversity and frequent runs of homozygosity (ROH). Therefore, the objective of the present study was to use 42,182 autosomal SNPs to identify genomic regions in 3,191 sheep from six commercial breeds subjected to selection pressure and to quantify the genetic diversity within each breed using ROH. In addition, the historical effective population size of each breed was also estimated and, in conjunction with ROH, was used to elucidate the demographic history of the six breeds. ROH were common in the autosomes of animals in the present study, but the observed breed differences in patterns of ROH length and burden suggested differences in breed effective population size and recent management. ROH provided a sufficient predictor of the pedigree inbreeding coefficient, with an estimated correlation between both measures of 0.62. Genomic regions under putative selection were identified using two complementary algorithms; the fixation index and hapFLK. The identified regions under putative selection included candidate genes associated with skin pigmentation, body size and muscle formation; such characteristics are often sought after in modern-day breeding programs. These regions of selection frequently overlapped with high ROH regions both within and across breeds. Multiple yet uncharacterised genes also resided within putative regions of selection. This further substantiates the need for a more comprehensive annotation of the sheep genome as these uncharacterised genes may contribute to traits of interest in the animal sciences. Despite this, the regions identified as under putative selection in the current study provide an insight into the mechanisms leading to breed differentiation and genetic variation in meat production.
[Mh] Termos MeSH primário: Homozigoto
Endogamia
Seleção Genética
Carneiro Doméstico/genética
[Mh] Termos MeSH secundário: Algoritmos
Animais
Domesticação
Frequência do Gene
Técnicas de Genotipagem
Carne
Modelos Genéticos
Polimorfismo de Nucleotídeo Único
Densidade Demográfica
Análise de Componente Principal
Recombinação Genética
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176780


  6 / 5443 MEDLINE  
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[PMID]:28938023
[Au] Autor:Klápste J; Suontama M; Telfer E; Graham N; Low C; Stovold T; McKinley R; Dungey H
[Ad] Endereço:Scion (New Zealand Forest Research Institute Ltd.), 49 Sala Street, 3046 Rotorua, New Zealand.
[Ti] Título:Exploration of genetic architecture through sib-ship reconstruction in advanced breeding population of Eucalyptus nitens.
[So] Source:PLoS One;12(9):e0185137, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accurate inference of relatedness between individuals in breeding population contributes to the precision of genetic parameter estimates, effectiveness of inbreeding management and the amount of genetic progress delivered from breeding programs. Pedigree reconstruction has been proven to be an efficient tool to correct pedigree errors and recover hidden relatedness in open pollinated progeny tests but the method can be limited by the lack of parental genotypes and the high proportion of alien pollen from outside the breeding population. Our study investigates the efficiency of sib-ship reconstruction in an advanced breeding population of Eucalyptus nitens with only partially tracked pedigree. The sib-ship reconstruction allowed the identification of selfs (4% of the sample) and the exploration of their potential effect on inbreeding depression in the traits studied. We detected signs of inbreeding depression in diameter at breast height and growth strain while no indications were observed in wood density, wood stiffness and tangential air-dry shrinkage. After the application of a corrected sib-ship relationship matrix, additive genetic variance and heritability were observed to increase where signs of inbreeding depression were initially detected. Conversely, the same genetic parameters for traits that appeared to be free of inbreeding depression decreased in size. It therefore appeared that greater genetic variance may be due, at least in part, to contributions from inbreeding in these studied populations rather than a removal of inbreeding as is traditionally thought.
[Mh] Termos MeSH primário: Eucalyptus/genética
Modelos Genéticos
Melhoramento Vegetal
[Mh] Termos MeSH secundário: Austrália
Variação Genética
Endogamia
Análise Multivariada
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185137


  7 / 5443 MEDLINE  
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[PMID]:28787007
[Au] Autor:Giacopuzzi E; Gennarelli M; Minelli A; Gardella R; Valsecchi P; Traversa M; Bonvicini C; Vita A; Sacchetti E; Magri C
[Ad] Endereço:Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
[Ti] Título:Exome sequencing in schizophrenic patients with high levels of homozygosity identifies novel and extremely rare mutations in the GABA/glutamatergic pathways.
[So] Source:PLoS One;12(8):e0182778, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inbreeding is a known risk factor for recessive Mendelian diseases and previous studies have suggested that it could also play a role in complex disorders, such as psychiatric diseases. Recent inbreeding results in the presence of long runs of homozygosity (ROHs) along the genome, which are also defined as autozygosity regions. Genetic variants in these regions have two alleles that are identical by descent, thus increasing the odds of bearing rare recessive deleterious mutations due to a homozygous state. A recent study showed a suggestive enrichment of long ROHs in schizophrenic patients, suggesting that recent inbreeding could play a role in the disease. To better understand the impact of autozygosity on schizophrenia risk, we selected, from a cohort of 180 Italian patients, seven subjects with extremely high numbers of large ROHs that were likely due to recent inbreeding and characterized the mutational landscape within their ROHs using Whole Exome Sequencing and, gene set enrichment analysis. We identified a significant overlap (17%; empirical p-value = 0.0171) between genes inside ROHs affected by low frequency functional homozygous variants (107 genes) and the group of most promising candidate genes mutated in schizophrenia. Moreover, in four patients, we identified novel and extremely rare damaging mutations in the genes involved in neurodevelopment (MEGF8) and in GABA/glutamatergic synaptic transmission (GAD1, FMN1, ANO2). These results provide insights into the contribution of rare recessive mutations and inbreeding as risk factors for schizophrenia. ROHs that are likely due to recent inbreeding harbor a combination of predisposing low-frequency variants and extremely rare variants that have a high impact on pivotal biological pathways implicated in the disease. In addition, this study confirms that focusing on patients with high levels of homozygosity could be a useful prioritization strategy for discovering new high-impact mutations in genetically complex disorders.
[Mh] Termos MeSH primário: Análise Mutacional de DNA
Exoma/genética
Ácido Glutâmico/metabolismo
Homozigoto
Esquizofrenia/genética
Ácido gama-Aminobutírico/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Feminino
Predisposição Genética para Doença/genética
Seres Humanos
Endogamia
Masculino
Meia-Idade
Mutação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3KX376GY7L (Glutamic Acid); 56-12-2 (gamma-Aminobutyric Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182778


  8 / 5443 MEDLINE  
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[PMID]:28700592
[Au] Autor:Zhang X; Liu X; Zhang D; Tang H; Sun B; Li C; Hao L; Liu C; Li Y; Shi Y; Xie X; Song Y; Wang T; Li Y
[Ad] Endereço:Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Genome-wide identification of gene expression in contrasting maize inbred lines under field drought conditions reveals the significance of transcription factors in drought tolerance.
[So] Source:PLoS One;12(7):e0179477, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drought is a major threat to maize growth and production. Understanding the molecular regulation network of drought tolerance in maize is of great importance. In this study, two maize inbred lines with contrasting drought tolerance were tested in the field under natural soil drought and well-watered conditions. In addition, the transcriptomes of their leaves was analyzed by RNA-Seq. In total, 555 and 2,558 genes were detected to specifically respond to drought in the tolerant and the sensitive line, respectively, with a more positive regulation tendency in the tolerant genotype. Furthermore, 4,700, 4,748, 4,403 and 4,288 genes showed differential expression between the two lines under moderate drought, severe drought and their well-watered controls, respectively. Transcription factors were enriched in both genotypic differentially expressed genes and specifically responsive genes of the tolerant line. It was speculated that the genotype-specific response of 20 transcription factors in the tolerance line and the sustained genotypically differential expression of 22 transcription factors might enhance tolerance to drought in maize. Our results provide new insight into maize drought tolerance-related regulation systems and provide gene resources for subsequent studies and drought tolerance improvement.
[Mh] Termos MeSH primário: Secas
Proteínas de Plantas/genética
Estresse Fisiológico
Fatores de Transcrição/genética
Transcriptoma
Zea mays/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Regulação da Expressão Gênica de Plantas
Genoma de Planta
Endogamia
Proteínas de Plantas/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179477


  9 / 5443 MEDLINE  
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[PMID]:28692127
[Au] Autor:Cabral JC; Baldoni AB; Tonini H; Azevedo VCR; Giustina LD; Tiago AV; Rossi AAB
[Ad] Endereço:Laboratório de Genética Vegetal e Biologia Molecular, Faculdade de Ciências Biológicas e Agrárias, Centro de Tecnologia da Amazônia Meridional, Universidade do Estado de Mato Grosso, Campus de Alta Floresta, Alta Floresta, MT, Brasil.
[Ti] Título:Diversity and genetic structure of the native Brazil nut tree (Bertholletia excelsa Bonpl.) population.
[So] Source:Genet Mol Res;16(3), 2017 Jul 06.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The present study was carried out in a native Brazil nut tree population (Bertholletia excelsa Bonpl., Lecythidaceae) to assess its genetic diversity and structure. Ten microsatellite markers were used to genotype 198 adult trees (B. excelsa). The population presented high genetic diversity and inbreeding absence rates. The empirical Bayesian method showed three distinct groups in the structure of this population. Molecular analysis of variance showed 98% variability within groups, and 2% between groups. The genetic divergence (F ) indicated little difference between groups; thus, suggesting efficient gene flow between the analyzed B. excelsa adult trees.
[Mh] Termos MeSH primário: Bertholletia/genética
Genótipo
Polimorfismo Genético
[Mh] Termos MeSH secundário: Teorema de Bayes
Fluxo Gênico
Endogamia
Repetições de Microssatélites
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16039702


  10 / 5443 MEDLINE  
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[PMID]:28692118
[Au] Autor:Pereira GS; Pinho RGV; Pinho EVRV; Pires LPM; Bernardo Junior LAY; Pereira JLA; Melo MP
[Ad] Endereço:Programa de Pós-Graduação em Genética e Melhoramento de Plantas, Universidade Federal de Lavras, Lavras, MG, Brasil.
[Ti] Título:Selection of maize inbred lines and gene expression for resistance to ear rot.
[So] Source:Genet Mol Res;16(3), 2017 Jul 06.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:In recent years, there has been a large incidence of fungi causing "ear rot" in maize in Brazil, the main fungus being Fusarium verticillioides. The most efficient and competitive alternative for control of this disease consists of using maize hybrids resistant to this pathogen. Thus, the aims of this study were to analyze the genetic variability of maize inbred lines in regard to resistance to ear rot to observe if there is a maternal effect to resistance to ear rot, to study genetic control of the traits evaluated in hybrids originating from inbred lines of the maize breeding program at the Agriculture Department of Universidade Federal de Lavras (Lavras, MG, Brazil), and characterize the gene expression pattern related to the plant defense mechanism against F. verticillioides. High genetic availability was observed for resistance to this disease among the inbred lines evaluated. Considering combined diallel analysis, it was observed that the mean square of general combining ability (GCA) was not significant for the characteristic under study. However, specific combining ability (SCA) was significant, which indicates the predominance of non-additive effects involved in control of the characteristic for the population evaluated. A maternal effect was not observed for the characteristic of ear rot resistance in this study. Inbred lines 22, 58, and 91 showed potential for use in breeding programs aiming at resistance to F. verticillioides. Only two genes, LOX8 and Hsp82, had a satisfactory result that was able to be related to a plant defense mechanism when there is ear rot infection, though expression of these genes was observed in only one susceptible genotype. Thus, the genes LOX8 and Hsp82 are potential molecular markers for selection of maize inbred lines resistant to F. verticillioides.
[Mh] Termos MeSH primário: Melhoramento Vegetal
Imunidade Vegetal/genética
Seleção Genética
Zea mays/genética
[Mh] Termos MeSH secundário: Fusarium/patogenicidade
Regulação da Expressão Gênica de Plantas
Marcadores Genéticos
Variação Genética
Proteínas de Choque Térmico/genética
Endogamia
Lipoxigenase/genética
Herança Materna
Proteínas de Plantas/genética
Zea mays/imunologia
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Heat-Shock Proteins); 0 (Plant Proteins); EC 1.13.11.12 (Lipoxygenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16039415



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