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[PMID]: 29331744 [Au] Autor: Zhang X; Li P; Hua Y; Ji P; Yao W; Ma Q; Yuan Z; Wen Y; Yang C; Wei Y [Ad] Endereço: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. [Ti] Título: Urinary metabolomics study the mechanism of Taohong Siwu Decoction intervention in acute blood stasis model rats based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. [So] Source: J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:51-60, 2018 Feb 01. [Is] ISSN: 1873-376X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Taohong Siwu Decoction (TSD) is a classic prescription in traditional Chinese medicine and is widely used to promote blood circulation to remove blood stasis. However, the effect mechanisms are not yet well understood. Here, a urinary metabolomic approach based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC/Q-TOF-MS) was conducted to explore the changes in the endogenous metabolites and to assess the integral efficacy of TSD on acute blood stasis model rats. Then, parameters for hemorheology and coagulation functions were detected. Principal component analysis (PCA) and orthogonal partial least squares discriminate analysis (OPLS-DA) was used to investigate the global metabolite alterations and to evaluate the preventive effects of TSD in rats. Potential metabolite markers were found using OPLS-DA and t-test. Furthermore, metabolic pathway analysis was performed to construct metabolic networks. The results showed that TSD could significantly decrease whole blood viscosity and plasma viscosity. It also significantly prolonged partial thromboplastin time (APPT) and prothrombin time (PT), increased thrombin time (TT) and lowered fibrinogen content (FIB). Moreover, 24 potential metabolite markers of acute blood stasis were screened, and the levels were all reversed to different degrees after TSD administration. In metabolic networks, amino acid metabolism (arginine and proline metabolism; histidine metabolism; alanine, aspartate, and glutamate metabolism; phenylalanine, tyrosine, and tryptophan biosynthesis; phenylalanine metabolism) and lipid metabolism (glycerophospholipid metabolism; linoleic acid metabolism; alpha-linolenic acid metabolism) were closely related with the intervention mechanism of TSD on acute blood stasis. The urinary metabolomic approach can be applied to clarify the mechanism of TSD in promoting blood circulation to remove acute blood stasis and to provide the theoretical basis for further research on the therapeutic mechanism of TSD in clinical practice. [Mh] Termos MeSH primário: Biomarcadores/metabolismo Biomarcadores/urina Cromatografia Líquida/métodos Medicamentos de Ervas Chinesas/farmacocinética Metabolômica/métodos Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos [Mh] Termos MeSH secundário: Animais Feminino Hemorreologia Metaboloma Análise de Componente Principal Ratos Ratos Wistar [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Drugs, Chinese Herbal); 0 (Taohong Siwu decoction II) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180115 [St] Status: MEDLINE

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[PMID]: 29244812 [Au] Autor: Chang SS; Tu S; Baek KI; Pietersen A; Liu YH; Savage VM; Hwang SL; Hsiai TK; Roper M [Ad] Endereço: Department of Mathematics, University of California Los Angeles, Los Angeles, California, United States of America. [Ti] Título: Optimal occlusion uniformly partitions red blood cells fluxes within a microvascular network. [So] Source: PLoS Comput Biol;13(12):e1005892, 2017 12. [Is] ISSN: 1553-7358 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In animals, gas exchange between blood and tissues occurs in narrow vessels, whose diameter is comparable to that of a red blood cell. Red blood cells must deform to squeeze through these narrow vessels, transiently blocking or occluding the vessels they pass through. Although the dynamics of vessel occlusion have been studied extensively, it remains an open question why microvessels need to be so narrow. We study occlusive dynamics within a model microvascular network: the embryonic zebrafish trunk. We show that pressure feedbacks created when red blood cells enter the finest vessels of the trunk act together to uniformly partition red blood cells through the microvasculature. Using mathematical models as well as direct observation, we show that these occlusive feedbacks are tuned throughout the trunk network to prevent the vessels closest to the heart from short-circuiting the network. Thus occlusion is linked with another open question of microvascular function: how are red blood cells delivered at the same rate to each micro-vessel? Our analysis shows that tuning of occlusive feedbacks increase the total dissipation within the network by a factor of 11, showing that uniformity of flows rather than minimization of transport costs may be prioritized by the microvascular network. [Mh] Termos MeSH primário: Microcirculação/fisiologia Microvasos/fisiologia Modelos Cardiovasculares [Mh] Termos MeSH secundário: Animais Animais Geneticamente Modificados Velocidade do Fluxo Sanguíneo/fisiologia Biologia Computacional Eritrócitos/fisiologia Retroalimentação Fisiológica Hemorreologia Microvasos/anatomia & histologia Peixe-Zebra [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180216 [Lr] Data última revisão: 180216 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171216 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pcbi.1005892

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[PMID]: 29215846 [Au] Autor: Masljakov VV; Suhanova OA; Barsukov VG; Kurkin KG; Suhanov SA [Ti] Título: [Possibilities of correction rheological blood svoytv at chipped and cut wounds of the breast ]. [So] Source: Patol Fiziol Eksp Ter;61(2):72-5, 2017 Apr-Jun. [Is] ISSN: 0031-2991 [Cp] País de publicação: Russia (Federation) [La] Idioma: eng [Ab] Resumo: The purpose: research objective: to study influence of electromagnetic oscillations of millimetric range on rheological properties of blood at patients with chipped and cut wounds of a breast for the purpose of their correction. Methods. For the solution of a research objective we have carried out studying of changes of rheological properties of blood at the 22nd patient with the getting chipped and cut wounds of a breast without internal injury during the next postoperative period. All patient has executed primary surgical processing and drainage of a pleural cavity. At all patients the volume of blood loss has made 200-500 ml. Criteria of inclusion were: existence of the getting wound of a thorax, existence of a small gemotoraks. Criteria of an exception: blood loss existence more than 500 ml, existence of the combined and multiple damages. The main group is divided into two subgroups, in the first 12 patients with application of electromagnetic oscillations of millimetric range, have entered the second 10 people without application of electromagnetic oscillations of millimetric range. The group of comparison was made by 15 rather healthy donor volunteers of the same age and a floor. To all patients the hemotransfusion wasn't carried out, the volume of infusional therapy was comparable in both groups. Changes of a rheology of blood came to light by means of the accounting of viscosity of blood, change of an index of deformation and aggregation of erythrocytes. Conclusion. As a result of the conducted research it is established that application of electromagnetic oscillation of millimetric range for patients with chipped and cut wounds of a breast prevents development of changes of rheological properties of blood, at the same time patients well transfer this procedure that is shown by lack of side effects. [Mh] Termos MeSH primário: Hemorreologia Hemorragia/sangue Traumatismos Torácicos/fisiopatologia Ferimentos Penetrantes/sangue [Mh] Termos MeSH secundário: Hemorragia/fisiopatologia Hemorragia/cirurgia Seres Humanos Masculino Traumatismos Torácicos/sangue Traumatismos Torácicos/cirurgia Ferimentos Penetrantes/fisiopatologia Ferimentos Penetrantes/cirurgia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171221 [Lr] Data última revisão: 171221 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171208 [St] Status: MEDLINE

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[PMID]: 28741718 [Au] Autor: Nader E; Connes P; Lamarre Y; Renoux C; Joly P; Hardy-Dessources MD; Cannas G; Lemonne N; Ballas SK [Ad] Endereço: Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team "Vascular Biology and Red Blood Cell," Université Claude Bernard Lyon 1, Lyon, France. [Ti] Título: Plasmapheresis may improve clinical condition in sickle cell disease through its effects on red blood cell rheology. [So] Source: Am J Hematol;92(11):E629-E630, 2017 Nov. [Is] ISSN: 1096-8652 [Cp] País de publicação: United States [La] Idioma: eng [Mh] Termos MeSH primário: Síndrome Torácica Aguda/terapia Eritrócitos/metabolismo Hemorreologia Plasmaferese [Mh] Termos MeSH secundário: Síndrome Torácica Aguda/sangue Síndrome Torácica Aguda/genética Adulto Feminino Homozigoto Seres Humanos [Pt] Tipo de publicação: CASE REPORTS; LETTER [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171130 [Lr] Data última revisão: 171130 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170726 [St] Status: MEDLINE [do] DOI: 10.1002/ajh.24870

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[PMID]: 28694192 [Au] Autor: Kim SA; Sung JY; Woo CH; Choi HC [Ad] Endereço: Department of Pharmacology, College of Medicine, Yeungnam University, 170 Hyunchung-Ro, Nam-Gu, Daegu 42415, Republic of Korea. [Ti] Título: Laminar shear stress suppresses vascular smooth muscle cell proliferation through nitric oxide-AMPK pathway. [So] Source: Biochem Biophys Res Commun;490(4):1369-1374, 2017 Sep 02. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In healthy condition, vascular smooth muscle cells (VSMCs) are not directly exposed to shear stresses, because they are shielded by endothelial cell (EC) layer that lines blood vessels. After injury to EC layer caused by rupture of atherosclerotic lesions or invasive techniques such as angioplasty, VSMCs are directly exposed to blood flow which modulate molecular signaling and function. In endothelium, exposure to fluid shear stress has been reported to induce AMP-activated protein kinase (AMPK) phosphorylation and nitric oxide (NO) production. However, the influence of laminar shear stress on exposed VSMC is not defined. In this study, we investigated whether laminar shear stress regulates AMPK phosphorylation in VSMC and tried to identify underlying signaling pathway. NO production was increased by shear stress. The expression of NOS isoforms was increased 1 h after exposure to shear stress, and AMPK phosphorylation started to increase after 2 h. AMPK and LKB1, the upstream kinases of AMPK, phosphorylation were decreased by the non-selective NOS inhibitor l-NAME and the selective iNOS inhibitor aminoguanidine despite exposure to shear stress. On the other hand, compound C, a specific AMPK inhibitor, did not affect the expression of NOS isoforms. In addition, PDGF-induced VSMC proliferation was decreased by shear stress and restored by l-NAME. These findings suggest that shear stress upregulated AMPK phosphorylation in VSMC via NOS expression may be a beneficial route to prevent pathogenesis in the vascular system. [Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/genética Células Endoteliais/metabolismo Mecanotransdução Celular Óxido Nítrico Sintase Tipo II/genética Óxido Nítrico/biossíntese Proteínas Serina-Treonina Quinases/genética [Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/antagonistas & inibidores Proteínas Quinases Ativadas por AMP/metabolismo Animais Aorta Torácica/citologia Aorta Torácica/metabolismo Proliferação Celular Células Endoteliais/citologia Regulação da Expressão Gênica Guanidinas/farmacologia Hemorreologia Masculino Músculo Liso Vascular/citologia Músculo Liso Vascular/metabolismo NG-Nitroarginina Metil Éster/farmacologia Óxido Nítrico Sintase Tipo II/antagonistas & inibidores Óxido Nítrico Sintase Tipo II/metabolismo Fosforilação/efeitos dos fármacos Cultura Primária de Células Inibidores de Proteínas Quinases/farmacologia Proteínas Serina-Treonina Quinases/metabolismo Ratos Ratos Sprague-Dawley Estresse Mecânico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Guanidines); 0 (Protein Kinase Inhibitors); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, rat); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Stk11 protein, rat); EC 2.7.11.31 (AMP-Activated Protein Kinases); SCQ4EZQ113 (pimagedine); V55S2QJN2X (NG-Nitroarginine Methyl Ester) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170712 [St] Status: MEDLINE

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[PMID]: 28638872 [Au] Autor: Zhang JX; Feng Y; Zhang Y; Liu Y; Li SD; Yang MH [Ad] Endereço: TCM Department, PLA General Hospital, Beijing 100853, China. [Ti] Título: HEMORHEOLOGY INDEX CHANGES IN A RAT ACUTE BLOOD STASIS MODEL: A SYSTEMATIC REVIEW AND META-ANALYSIS. [So] Source: Afr J Tradit Complement Altern Med;14(4):96-107, 2017. [Is] ISSN: 2505-0044 [Cp] País de publicação: Nigeria [La] Idioma: eng [Ab] Resumo: BACKGROUND: Blood stasis has received increasing attention in research related to traditional Chinese medicine (TCM) and integrative Chinese and Western medicine. More than 90% of research studies use hemorheology indexes to evaluate the establishment of animal blood stasis models rather than pathological methods, as hemorheology index evaluations of blood stasis were short of the consolidated standard. The aim of this study was to evaluate the accuracy of hemorheology indexes in rat models of acute blood stasis (ABS) based on studies in which the ABS model had been confirmed by pathological methods. MATERIALS AND METHODS: We searched the Chinese National Knowledge Infrastructure database (CNKI), Chinese Medical Journal Database (CMJD), Chinese Biology Medicine disc (CBM), Wanfang database, and PubMed for studies of rat blood stasis models; the search identified 18 studies of rat ABS models induced by subcutaneous injection of epinephrine combined with an ice bath. Each included study received a modified Collaborative Approach to Meta-Analysis and Review of Animal Data from Experimental Studies (CAMARADES) score list and methodological quality assessment, then data related to whole blood viscosity, plasma viscosity, platelet aggregation rate, erythrocyte aggregation index, and fibrinogen concentration were extracted. Extracted data were analyzed using Revman 5.3; heterogeneity was tested using Egger's test. RESULTS: A total of 343 studies of rat blood stasis were reviewed. Eighteen studies were included in this meta-analysis; the mean CAMARADES score was 3.5. The rat ABS model revealed a significant increase in whole blood viscosity (medium shear rate), whole blood viscosity (high shear rate), plasma viscosity, platelet aggregation rate, erythrocyte aggregation index, and fibrinogen concentration compared to controls, with weighted mean differences (WMD) of 2.42 mPa/s (95% confidence interval (CI) = 1.73 - 3.10); 1.76 mPa/s (95% CI = 1.28 - 2.24); 0.39 mPa/s (95% CI = 0.24 - 0.55); 13.66% (95% CI = 9.78 - 17.55); 0.84 (95% CI = 0.53 - 1.16); and 1.22 g/L (95% CI = 0.76 - 1.67), respectively. Subgroup analysis showed that whole blood viscosity, plasma viscosity, and the platelet aggregation rate test methods were more sensitive when measured at 0-24 h than at 24-72 h after induction of blood stasis. CONCLUSIONS: Rat blood stasis studies have incomplete experimental design and quality controls, and thus need an integrated improvement. Meta-analysis of included studies indicated that the unified hemorheology index of whole blood viscosity (medium and high shear rate), platelet aggregation rate, erythrocyte aggregation rate, and fibrinogen concentration might be used for assessment of rat ABS models independent of pathology methods. [Mh] Termos MeSH primário: Doenças Hematológicas/diagnóstico [Mh] Termos MeSH secundário: Doença Aguda Animais Modelos Animais de Doenças Doenças Hematológicas/sangue Doenças Hematológicas/patologia Hemorreologia Seres Humanos Medicina Tradicional Chinesa Ratos [Pt] Tipo de publicação: JOURNAL ARTICLE; META-ANALYSIS; REVIEW [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170623 [St] Status: MEDLINE [do] DOI: 10.21010/ajtcam.v14i4.12

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[PMID]: 28587739 [Au] Autor: Berdajs D; Mosbahi S; Ferrari E; Charbonnier D; von Segesser LK [Ad] Endereço: Department of Cardiac Surgery, University Hospital Basel, Basel; Department of Surgery and Anesthesiology, Cardiovascular Research, University Hospital Lausanne, Lausanne. Electronic address: denis.berdajs@bluewin.ch. [Ti] Título: Aortic Valve Pathology as a Predictive Factor for Acute Aortic Dissection. [So] Source: Ann Thorac Surg;104(4):1340-1348, 2017 Oct. [Is] ISSN: 1552-6259 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: BACKGROUND: In this study, the effect of aortic valve (AV) pathology on local hemodynamic conditions was evaluated as a potential trigger for the onset of acute type A and B aortic dissection. METHODS: A time- and pressure-related four-dimensional (4-D) computed fluid dynamic model of the aorta was established. In an experimental setup, AV stenosis and AV insufficiency were created. 4-D pressure-related geometry of the aortic root (AR) with valve insufficiency and valve stenosis were determined by high-fidelity (200 Hz) microsonometric crystals. Flow and pressure were obtained at the left ventricle, ascending aorta, and aortic arch. RESULTS: Expansion of the AR in AV insufficiency was higher with expansion in AV stenosis, at peak ejection, and at the end of systole. In AV insufficiency, there was low shear stress (0 to 0.6 Pa), turbulent flow, and high pressure (80 to 95 mm Hg) at the anterior wall of the ascending aorta, at the proximal aortic arch, and at the aortic isthmus. In stenosis, high shear stress (>2 Pa) and high pressure (>95 mm Hg) were found at the ascending aorta and at the bifurcation of the brachiocephalic trunk. CONCLUSIONS: In AV insufficiency, low shear stresses and turbulent flow regions were documented at the traditional levels of entry tears for acute type A and B dissection. In AV stenosis, high shear stress with elevated pressure at the ascending aorta may be a trigger element for vessel dilatation, aneurysm formation, and intimal tear, which is typical for type A aortic dissection. [Mh] Termos MeSH primário: Aneurisma Dissecante/etiologia Aneurisma Aórtico/etiologia Insuficiência da Valva Aórtica/complicações Estenose da Valva Aórtica/complicações Valva Aórtica/patologia [Mh] Termos MeSH secundário: Valva Aórtica/fisiopatologia Insuficiência da Valva Aórtica/patologia Insuficiência da Valva Aórtica/fisiopatologia Estenose da Valva Aórtica/patologia Estenose da Valva Aórtica/fisiopatologia Fenômenos Biomecânicos Hemorreologia Seres Humanos Modelos Cardiovasculares [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171010 [Lr] Data última revisão: 171010 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 170608 [St] Status: MEDLINE

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[PMID]: 28580719 [Au] Autor: Nair PM; Pandya SG; Dallo SF; Reddoch KM; Montgomery RK; Pidcoke HF; Cap AP; Ramasubramanian AK [Ad] Endereço: Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, TX, USA. [Ti] Título: Platelets stored at 4°C contribute to superior clot properties compared to current standard-of-care through fibrin-crosslinking. [So] Source: Br J Haematol;178(1):119-129, 2017 Jul. [Is] ISSN: 1365-2141 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Currently, platelets for transfusion are stored at room temperature (RT) for 5-7 days with gentle agitation, but this is less than optimal because of loss of function and risk of bacterial contamination. We have previously demonstrated that cold (4°C) storage is an attractive alternative because it preserves platelet metabolic reserves, in vitro responses to agonists of activation, aggregation and physiological inhibitors, as well as adhesion to thrombogenic surfaces better than RT storage. Recently, the US Food and Drug Administration clarified that apheresis platelets stored at 4°C for up to 72 h may be used for treating active haemorrhage. In this work, we tested the hypothesis that cold-stored platelets contribute to generating clots with superior mechanical properties compared to RT-stored platelets. Rheological studies demonstrate that the clots formed from platelets stored at 4°C for 5 days are significantly stiffer (higher elastic modulus) and stronger (higher critical stress) than those formed from RT-stored platelets. Morphological analysis shows that clot fibres from cold-stored platelets were denser, thinner, straighter and with more branch points or crosslinks than those from RT-stored platelets. Our results also show that the enhanced clot strength and packed structure is due to cold-induced plasma factor XIII binding to platelet surfaces, and the consequent increase in crosslinking. [Mh] Termos MeSH primário: Plaquetas/fisiologia Preservação de Sangue/métodos Agregação Plaquetária/fisiologia [Mh] Termos MeSH secundário: Plaquetas/metabolismo Plaquetas/ultraestrutura Adesão Celular/fisiologia Fator XIII/metabolismo Fibrina/metabolismo Hemorreologia/fisiologia Seres Humanos Microscopia Eletrônica de Varredura/métodos Refrigeração Temperatura Ambiente Trombina/biossíntese [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 9001-31-4 (Fibrin); 9013-56-3 (Factor XIII); EC 3.4.21.5 (Thrombin) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170906 [Lr] Data última revisão: 170906 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170606 [St] Status: MEDLINE [do] DOI: 10.1111/bjh.14751

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[PMID]: 28511905 [Au] Autor: Marini MA; Fiorentino TV; Andreozzi F; Mannino GC; Succurro E; Sciacqua A; Perticone F; Sesti G [Ad] Endereço: Department of Systems Medicine, University of Rome Tor Vergata, 00133 Rome, Italy. [Ti] Título: Hemorheological alterations in adults with prediabetes identified by hemoglobin A1c levels. [So] Source: Nutr Metab Cardiovasc Dis;27(7):601-608, 2017 Jul. [Is] ISSN: 1590-3729 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: BACKGROUND AND AIMS: A link between increased blood viscosity and type 2 diabetes has been previously reported. Herein, we investigated the association of blood viscosity with prediabetes, identified by glycated hemoglobin A1c (HbA1c) according to the new American Diabetes Association criteria, and subclinical atherosclerosis. METHODS AND RESULTS: The study cohort includes 1136 non-diabetic adults submitted to anthropometrical evaluation, an oral glucose tolerance test and ultrasound measurement of carotid intima-media thickness (IMT). Whole blood viscosity was estimated using a validated formula based on hematocrit and total plasma proteins. After adjusting for age, and gender, individuals with HbA1c-defined prediabetes (HbA1c 5.7-6.4% [39-47 mmol/mol]) exhibited significantly higher values of hematocrit, and predicted blood viscosity as compared with controls. Increased levels of IMT were observed in subjects with HbA1c-defined prediabetes in comparison to controls. Predicted blood viscosity was positively correlated with age, waist circumference, blood pressure, cholesterol, triglycerides, fibrinogen, white blood cell, HbA1c, fasting and 2-h post-load glucose levels, fasting insulin, IMT and inversely correlated with HDL and Matsuda index of insulin sensitivity. Of the three glycemic parameters, i.e. HbA1c, fasting and 2-h post-load glucose, only HbA1c showed a significant correlation with predicted blood viscosity (ß = 0.054, P = 0.04) in a multivariate regression analysis model including multiple atherosclerosis risk factors. CONCLUSION: The study shows that individuals with HbA1c-defined prediabetes have increased predicted blood viscosity and IMT. The HbA1c criterion may be helpful to capture individuals with an increased risk of diabetes and cardiovascular disease who may benefit from an intensive lifestyle intervention. [Mh] Termos MeSH primário: Viscosidade Sanguínea Doenças Cardiovasculares/etiologia Hemoglobina A Glicada/análise Hemorreologia Estado Pré-Diabético/sangue Estado Pré-Diabético/fisiopatologia [Mh] Termos MeSH secundário: Adulto Biomarcadores/sangue Doenças Cardiovasculares/sangue Doenças Cardiovasculares/diagnóstico por imagem Doenças Cardiovasculares/fisiopatologia Espessura Intima-Media Carotídea Estudos de Casos e Controles Estudos Transversais Feminino Teste de Tolerância a Glucose Hematócrito Seres Humanos Masculino Meia-Idade Estado Pré-Diabético/complicações Estado Pré-Diabético/diagnóstico Valor Preditivo dos Testes Prognóstico Fatores de Risco [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Glycated Hemoglobin A); 0 (hemoglobin A1c protein, human) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170518 [St] Status: MEDLINE

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[PMID]: 28350986 [Au] Autor: Nakajima H; Yamamoto K; Agarwala S; Terai K; Fukui H; Fukuhara S; Ando K; Miyazaki T; Yokota Y; Schmelzer E; Belting HG; Affolter M; Lecaudey V; Mochizuki N [Ad] Endereço: Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. [Ti] Título: Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance. [So] Source: Dev Cell;40(6):523-536.e6, 2017 Mar 27. [Is] ISSN: 1878-1551 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Endothelial cells (ECs) line the inside of blood vessels and respond to mechanical cues generated by blood flow. Mechanical stimuli regulate the localization of YAP by reorganizing the actin cytoskeleton. Here we demonstrate blood-flow-mediated regulation of endothelial YAP in vivo. We indirectly monitored transcriptional activity of Yap1 (zebrafish YAP) and its spatiotemporal localization in living zebrafish and found that Yap1 entered the nucleus and promoted transcription in response to blood flow. In cultured human ECs, laminar shear stress induced nuclear import of YAP and its transcriptional activity in a manner independent of Hippo signaling. We uncovered a molecular mechanism by which flow induced the nuclear translocation of YAP through the regulation of filamentous actin and angiomotin. Yap1 mutant zebrafish showed a defect in vascular stability, indicating an essential role for Yap1 in blood vessels. Our data imply that endothelial Yap1 functions in response to flow to maintain blood vessels. [Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/metabolismo Vasos Sanguíneos/metabolismo Células Endoteliais/metabolismo Hemorreologia Fosfoproteínas/metabolismo Transativadores/metabolismo Proteínas de Peixe-Zebra/metabolismo [Mh] Termos MeSH secundário: Actinas/metabolismo Animais Núcleo Celular/metabolismo Células Endoteliais da Veia Umbilical Humana/metabolismo Seres Humanos Perfusão Transporte Proteico Proteínas Serina-Treonina Quinases/metabolismo Resistência ao Cisalhamento Transdução de Sinais/genética Estresse Mecânico Transcrição Genética Ativação Transcricional/genética Peixe-Zebra/embriologia Peixe-Zebra/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Actins); 0 (Adaptor Proteins, Signal Transducing); 0 (Phosphoproteins); 0 (Trans-Activators); 0 (YAP1 (Yes-associated) protein, human); 0 (Yes-associated protein (yap), zebrafish); 0 (Zebrafish Proteins); 0 (angiomotin protein, zebrafish); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (hippo protein, zebrafish) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170627 [Lr] Data última revisão: 170627 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170329 [St] Status: MEDLINE

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