Base de dados : MEDLINE
Pesquisa : E05.830.666 [Categoria DeCS]
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  1 / 5886 MEDLINE  
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[PMID]:29377915
[Au] Autor:VanInsberghe M; Zahn H; White AK; Petriv OI; Hansen CL
[Ad] Endereço:Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.
[Ti] Título:Highly multiplexed single-cell quantitative PCR.
[So] Source:PLoS One;13(1):e0191601, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and measurement in twenty separate qPCR reactions. Each of these steps is performed in parallel on up to 200 single cells per run. Experiments performed on dilutions of purified RNA establish assay linearity over a dynamic range of at least 104, a qPCR precision of 15%, and detection sensitivity down to a single cDNA molecule. We demonstrate the application of our device for rapid profiling of microRNA expression in single cells. Measurements performed on a panel of twenty miRNAs in two types of cells revealed clear cell-to-cell heterogeneity, with evidence of spontaneous differentiation manifested as distinct expression signatures. Highly multiplexed microfluidic RT-qPCR fills a gap in current capabilities for single-cell analysis, providing a rapid and cost-effective approach for profiling panels of marker genes, thereby complementing single-cell genomics methods that are best suited for global analysis and discovery. We expect this approach to enable new studies requiring fast, cost-effective, and precise measurements across hundreds of single cells.
[Mh] Termos MeSH primário: Reação em Cadeia da Polimerase Multiplex/métodos
[Mh] Termos MeSH secundário: Limite de Detecção
Microfluídica/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191601


  2 / 5886 MEDLINE  
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[PMID]:29364930
[Au] Autor:Tang M; Zhang M; Yan S; Xia L; Yang Z; Du C; Cui HL; Wei D
[Ad] Endereço:Chongqing Key laboratory of Multi-Scale manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.
[Ti] Título:Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures.
[So] Source:PLoS One;13(1):e0191515, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA oligonucleotides with a 5-base mutation at the 3'-terminus were investigated by terahertz (THz) spectroscopy in a marker-free manner. The four single-stranded oligonucleotides with 17nt have been detected with specificity on a microfluidic chip, and corroborated by spectral measurements with split-ring resonators. The number of hydrogen bonds formed between the oligonucleotide and its surrounding water molecules, deemed a key contribution to the THz absorption of biological solutions, was explored by molecular dynamics simulations to explain the experimental findings. Our work underlies the feasibility of THz spectroscopy combined with microstructures for marker-free detection of DNA, which may form the basis of a prospective diagnostic tool for studying genic mutation.
[Mh] Termos MeSH primário: DNA/química
Mutação
Espectroscopia Terahertz/métodos
[Mh] Termos MeSH secundário: DNA/genética
Microfluídica
Simulação de Dinâmica Molecular
Conformação de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191515


  3 / 5886 MEDLINE  
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[PMID]:28471354
[Au] Autor:Li D; Jacobsen MM; Gyune Rim N; Backman D; Kaplan DL; Wong JY
[Ad] Endereço:Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, United States of America.
[Ti] Título:Introducing biomimetic shear and ion gradients to microfluidic spinning improves silk fiber strength.
[So] Source:Biofabrication;9(2):025025, 2017 May 31.
[Is] ISSN:1758-5090
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Silkworm silk is an attractive biopolymer for biomedical applications due to its high mechanical strength and biocompatibility; as a result, there is increasing interest in scalable devices to spin silk and recombinant silk so as to improve and customize their properties for diverse biomedical purposes (Vepari and Kaplan 2007 Prog. Polym. Sci. 32 ). While artificial spinning of regenerated silk fibroins adds tunability to properties such as degradation rate and surface functionalization, the resulting fibers do not yet approach the mechanical strength of native silkworm silk. These drawbacks reduce the applicability and attractiveness of artificial silk (Kinahan et al 2011 Biomacromolecules 12 ). Here, we used computational fluid dynamic simulations to incorporate shear in tandem with biomimetic ion gradients by coupling a modular novel glass microfluidic device to our previous co-axial flow device. Fibers spun with this combined apparatus demonstrated a significant increase in mechanical strength compared to fibers spun with the basic apparatus alone, with a three-fold increase in Young's modulus and extensibility and a twelve-fold increase in toughness. These results thus demonstrate the critical importance of ionic milieu and shear stress in spinning strong fibers from solubilized silk fibroin.
[Mh] Termos MeSH primário: Biomimética/métodos
Microfluídica/métodos
Seda/química
Resistência à Tração
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Biomimética/instrumentação
Simulação por Computador
Hidrodinâmica
Íons
Metais/química
Microfluídica/instrumentação
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ions); 0 (Metals); 0 (Silk)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1088/1758-5090/aa711b


  4 / 5886 MEDLINE  
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[PMID]:29231937
[Au] Autor:Ugrinic M; Zambrano A; Berger S; Mann S; Tang TD; deMello A
[Ad] Endereço:Department of Chemistry & Applied Biosciences, ETH Zurich, Vladimir Prelog Weg 1, 8093 Zurich, Switzerland. andrew.demello@chem.ethz.ch.
[Ti] Título:Microfluidic formation of proteinosomes.
[So] Source:Chem Commun (Camb);54(3):287-290, 2018 Jan 02.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herein we describe a novel microfluidic method for the generation of proteinosome micro-droplets, based on bovine serum albumin and glucose oxidase conjugated to PNIPAAm chains. The size of such water-in-oil droplets is regulated via control of the input reagent flow rate, with generated proteinosome populations exhibiting narrower size distributions than those observed when using standard bulk methodologies. Importantly, proteinosomes transferred from an oil to an aqueous-environment remain intact, become fully hydrated and exhibit an increase in average size. Moreover, functional proteinosomes prepared via microfluidics exhibit lower K values and higher enzymatic activities than proteinosomes produced by bulk methodologies.
[Mh] Termos MeSH primário: Células Artificiais/química
Glucose Oxidase/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Animais
Bovinos
Fluoresceína-5-Isotiocianato/química
Peroxidase do Rábano Silvestre/química
Microfluídica
Tamanho da Partícula
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 25189-55-3 (poly-N-isopropylacrylamide); 27432CM55Q (Serum Albumin, Bovine); EC 1.1.3.4 (Glucose Oxidase); EC 1.11.1.- (Horseradish Peroxidase); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1039/c7cc08466h


  5 / 5886 MEDLINE  
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[PMID]:28453962
[Au] Autor:Iswardy E; Tsai TC; Cheng IF; Ho TC; Perng GC; Chang HC
[Ad] Endereço:Institute of Biomedical Engineering, National Cheng Kung University, Tainan 70101, Taiwan; Department of Physics, Syiah Kuala University, Banda Aceh 23111, Indonesia.
[Ti] Título:A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection.
[So] Source:Biosens Bioelectron;95:174-180, 2017 Sep 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The proof of concept of utilizing a microfluidic dielectrophoresis (DEP) chip was conducted to rapidly detect a dengue virus (DENV) in vitro based on the fluorescence immunosensing. The mechanism of detection was that the DEP force was employed to capture the modified beads (mouse anti-flavivirus monoclonal antibody-coated beads) in the microfluidic chip and the DENV modified with fluorescence label, as the detection target, can be then captured on the modified beads by immunoreaction. The fluorescent signal was then obtained through fluorescence microscopy, and then quantified by ImageJ freeware. The platform can accelerate an immuno-reaction time, in which the on-chip detection time was 5min, and demonstrating an ability for DENV detection as low as 10 PFU/mL. Furthermore, the required volume of DENV samples dramatically reduced, from the commonly used ~50µL to ~15µL, and the chip was reusable (>50x). Overall, this platform provides a rapid detection (5min) of the DENV with a low sample volume, compared to conventional methods. This proof of concept with regard to a microfluidic dielectrophoresis chip thus shows the potential of immunofluorescence based-assay applications to meet diagnostic needs.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Vírus da Dengue/isolamento & purificação
Dengue/diagnóstico
Microfluídica
[Mh] Termos MeSH secundário: Bioensaio
Dengue/virologia
Vírus da Dengue/genética
Vírus da Dengue/patogenicidade
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 5886 MEDLINE  
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[PMID]:29243668
[Au] Autor:Jin C; Hokmabad BV; Baldwin KA; Maass CC
[Ad] Endereço:Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
[Ti] Título:Chemotactic droplet swimmers in complex geometries.
[So] Source:J Phys Condens Matter;30(5):054003, 2018 Feb 07.
[Is] ISSN:1361-648X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemotaxis and auto-chemotaxis are key mechanisms in the dynamics of micro-organisms, e.g. in the acquisition of nutrients and in the communication between individuals, influencing the collective behaviour. However, chemical signalling and the natural environment of biological swimmers are generally complex, making them hard to access analytically. We present a well-controlled, tunable artificial model to study chemotaxis and autochemotaxis in complex geometries, using microfluidic assays of self-propelling oil droplets in an aqueous surfactant solution (Herminghaus et al 2014 Soft Matter 10 7008-22; Krüger et al 2016 Phys. Rev. Lett. 117). Droplets propel via interfacial Marangoni stresses powered by micellar solubilisation. Moreover, filled micelles act as a chemical repellent by diffusive phoretic gradient forces. We have studied these chemotactic effects in a series of microfluidic geometries, as published in Jin et al (2017 Proc. Natl Acad. Sci. 114 5089-94): first, droplets are guided along the shortest path through a maze by surfactant diffusing into the maze from the exit. Second, we let auto-chemotactic droplet swimmers pass through bifurcating microfluidic channels and record anticorrelations between the branch choices of consecutive droplets. We present an analytical Langevin model matching the experimental data. In a previously unpublished experiment, pillar arrays of variable sizes and shapes provide a convex wall interacting with the swimmer and, in the case of attachment, bending its trajectory and forcing it to revert to its own trail. We observe different behaviours based on the interplay of wall curvature and negative autochemotaxis, i.e. no attachment for highly curved interfaces, stable trapping at large pillars, and a narrow transition region where negative autochemotaxis makes the swimmers detach after a single orbit.
[Mh] Termos MeSH primário: Quimiotaxia
Tensoativos/química
[Mh] Termos MeSH secundário: Bactérias
Difusão
Microfluídica
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Surface-Active Agents); 059QF0KO0R (Water)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1088/1361-648X/aaa208


  7 / 5886 MEDLINE  
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[PMID]:27775555
[Au] Autor:Chretiennot T; Dubuc D; Grenier K
[Ad] Endereço:LAAS-CNRS, Université de Toulouse, CNRS, Toulouse 31031, France. thomas.chretiennot@gmail.com.
[Ti] Título:Microwave-Based Microfluidic Sensor for Non-Destructive and Quantitative Glucose Monitoring in Aqueous Solution.
[So] Source:Sensors (Basel);16(10), 2016 Oct 19.
[Is] ISSN:1424-8220
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This paper presents a reliable microwave and microfluidic miniature sensor dedicated to the measurement of glucose concentration in aqueous solution. The device; which is integrated with microtechnologies; is made of a bandstop filter implemented in a thin film microstrip technology combined with a fluidic microchannel. Glucose aqueous solutions have been characterized for concentration ranging from 80 g/L down to 0.3 g/L and are identified with the normalized insertion loss at optimal frequency. The sensitivity of the sensor has consequently been estimated at 7.6 × 10 dB/(g/L); together with the experimental uncertainty; the resolution of the sensor comes to 0.4 g/L. These results demonstrate the potentialities of such a sensor for the quantitative analysis of glucose in aqueous solution.
[Mh] Termos MeSH primário: Glucose/análise
Microfluídica/métodos
Micro-Ondas
Água/química
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 5886 MEDLINE  
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[PMID]:29320488
[Au] Autor:Muhammad N; Nadeem S; Mustafa MT
[Ad] Endereço:Department of Mathematics, Quaid-I-Azam University 45320, Islamabad 44000, Pakistan.
[Ti] Título:Analysis of ferrite nanoparticles in the flow of ferromagnetic nanofluid.
[So] Source:PLoS One;13(1):e0188460, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Theoretical analysis has been carried out to establish the heat transport phenomenon of six different ferromagnetic MnZnFe2O4-C2H6O2 (manganese zinc ferrite-ethylene glycol), NiZnFe2O4-C2H6O2 (Nickel zinc ferrite-ethylene glycol), Fe2O4-C2H6O2 (magnetite ferrite-ethylene glycol), NiZnFe2O4-H2O (Nickel zinc ferrite-water), MnZnFe2O4-H2O (manganese zinc ferrite-water), and Fe2O4-H2O (magnetite ferrite-water) nanofluids containing manganese zinc ferrite, Nickel zinc ferrite, and magnetite ferrite nanoparticles dispersed in a base fluid of ethylene glycol and water mixture. The performance of convective heat transfer is elevated in boundary layer flow region via nanoparticles. Magnetic dipole in presence of ferrites nanoparticles plays a vital role in controlling the thermal and momentum boundary layers. In perspective of this, the impacts of magnetic dipole on the nano boundary layer, steady, and laminar flow of incompressible ferromagnetic nanofluids are analyzed in the present study. Flow is caused by linear stretching of the surface. Fourier's law of heat conduction is used in the evaluation of heat flux. Impacts of emerging parameters on the magneto-thermomechanical coupling are analyzed numerically. Further, it is evident that Newtonian heating has increasing behavior on the rate of heat transfer in the boundary layer. Comparison with available results for specific cases show an excellent agreement.
[Mh] Termos MeSH primário: Compostos Férricos/química
Magnetismo
Nanopartículas Metálicas/química
Microfluídica
[Mh] Termos MeSH secundário: Modelos Teóricos
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ferric Compounds); 1317-54-0 (ferrite)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188460


  9 / 5886 MEDLINE  
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[PMID]:29198118
[Au] Autor:Pang B; Ding X; Wang G; Zhao C; Xu Y; Fu K; Sun J; Song X; Wu W; Liu Y; Song Q; Hu J; Li J; Mu Y
[Ad] Endereço:Department of Hygienic Inspection, School of Public Health, Jilin University , Changchun, Jilin 130021, People's Republic of China.
[Ti] Título:Rapid and Quantitative Detection of Vibrio parahemolyticus by the Mixed-Dye-Based Loop-Mediated Isothermal Amplification Assay on a Self-Priming Compartmentalization Microfluidic Chip.
[So] Source:J Agric Food Chem;65(51):11312-11319, 2017 Dec 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of bacterial food-poisoning events worldwide, which could be reduced using a promising on-site detection method. Herein, a rapid and quantitative method for VP detection was developed by applying a mixed-dye-loaded loop-mediated isothermal amplification (LAMP) assay on a self-priming compartmentalization (SPC) microfluidic chip, termed on-chip mixed-dye-based LAMP (CMD-LAMP). In comparison to conventional approaches, CMD-LAMP was advantageous on the limit of detection, which reached down to 1 × 10 CFU/mL in food-contaminated samples without the pre-enrichment of bacteria. Additionally, as a result of the use of a mixed dye and SPC chip, the quantitative result could be easily acquired, avoiding the requirement of sophisticated instruments and tedious operation. Also, CMD-LAMP was rapid and cost-effective. Conclusively, CMD-LAMP has great potential in realizing the on-site quantitative analysis of VP for food safety.
[Mh] Termos MeSH primário: Microfluídica/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
Vibrio parahaemolyticus/isolamento & purificação
[Mh] Termos MeSH secundário: Primers do DNA/genética
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Limite de Detecção
Microfluídica/instrumentação
Técnicas de Amplificação de Ácido Nucleico/instrumentação
Vibrio parahaemolyticus/genética
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03655


  10 / 5886 MEDLINE  
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[PMID]:29236762
[Au] Autor:Borne F; Kasimatis KR; Phillips PC
[Ad] Endereço:Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, United States of America.
[Ti] Título:Quantifying male and female pheromone-based mate choice in Caenorhabditis nematodes using a novel microfluidic technique.
[So] Source:PLoS One;12(12):e0189679, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pheromone cues are an important component of intersexual communication, particularly in regards to mate choice. Caenorhabditis nematodes predominant rely on pheromone production for mate finding and mate choice. Here we describe a new microfluidic paradigm for studying mate choice in nematodes. Specifically, the Pheromone Arena allows for a constant flow of odorants, including pheromones and other small molecules, to be passed in real time from signaling worms to those making a choice without any physical contact. We validated this microfluidic paradigm by corroborating previous studies in showing that virgin C. remanei and C. elegans males have a strong preference for virgin females over mated ones. Moreover, our results suggest that the strength of attraction is an additive effect of male receptivity and female signal production. We also explicitly examine female choice and find that females are more attracted to virgin males. However, a female's mate choice is strongly dependent on her mating status.
[Mh] Termos MeSH primário: Caenorhabditis elegans/fisiologia
Microfluídica
Atrativos Sexuais/análise
Comportamento Sexual Animal
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sex Attractants)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189679



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