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  1 / 41945 MEDLINE  
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[PMID]:29279438
[Au] Autor:Ebitani M; Ebitani T
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University.
[Ti] Título:[Rotational Isomers of Diphenhydramine].
[So] Source:Yakugaku Zasshi;138(3):417-424, 2018 Mar 01.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo: Diphenhydramine (DP), an antihistaminic agent, may become colored and daker or more fluorescent during storage. Herein, we spectroscopically examined the causes of this phenomenon under various DP storage conditions and durations. The infrared vibration-rotation spectrum shows multiple Gauche (G)-type conformers with different intramolecular n→π interaction strengths. The splitting pattern of the dimethylamino group protons in the H-NMR spectrum indicates that DP is mainly in the G-type with a small portion in the Trans (T)-type. The correlation between the red-shifted peak intensity in the UV•VIS absorbance spectrum and the coloring progression indicates a decreased intramolecular n→π interaction of the G-type under elevated temperature during storage. Enhanced fluorescence detected in the Excitation•Fluorescence spectrum demonstrates G-type (quenching) to T-type (fluorescent) conformation conversion, which is due to activated internal rotation of the dimethylamino group under elevated storage temperature and electronic excitation in the phenyl groups under light irradiation during storage. A signal detected in the ESR spectrum corresponds to the G-type charge transfer (CT) structure wherein part of the nonbonding electron pair on the N atom is intramolecularly redistributed to the phenyl groups. The CT structure presents the G-type quenching characteristics, whereas weak CT bonding corresponds to coloring. The results indicate that the quenching G-type is converted to T-type by heat or light to become color faded and bright with enhanced fluorescence and that T-type is reverted to G-type after storage under cool and dark conditions or by vacuum distillation to lose fluorescence.
[Mh] Termos MeSH primário: Difenidramina/química
Estabilidade de Medicamentos
Armazenamento de Medicamentos
Antagonistas dos Receptores Histamínicos/química
[Mh] Termos MeSH secundário: Cor
Espectroscopia de Ressonância de Spin Eletrônica
Fluorescência
Isomerismo
Luz
Espectroscopia de Ressonância Magnética
Conformação Molecular
Rotação
Espectrometria de Fluorescência
Espectrofotometria Infravermelho
Análise Espectral
Temperatura Ambiente
Vibração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histamine Antagonists); 8GTS82S83M (Diphenhydramine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.17-00175


  2 / 41945 MEDLINE  
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[PMID]:29238190
[Au] Autor:Salade L; Wauthoz N; Deleu M; Vermeersch M; De Vriese C; Amighi K; Goole J
[Ad] Endereço:Laboratoire de Pharmacie Galénique et de Biopharmacie, Université libre de Bruxelles (ULB), Brussels.
[Ti] Título:Development of coated liposomes loaded with ghrelin for nose-to-brain delivery for the treatment of cachexia.
[So] Source:Int J Nanomedicine;12:8531-8543, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to develop a ghrelin-containing formulation based on liposomes coated with chitosan intended for nose-brain delivery for the treatment of cachexia. Among the three types of liposomes developed, anionic liposomes provided the best results in terms of encapsulation efficiency (56%) and enzymatic protection against trypsin (20.6% vs 0% for ghrelin alone) and carboxylesterase (81.6% vs 17.2% for ghrelin alone). Ghrelin presented both electrostatic and hydrophobic interactions with the anionic lipid bilayer, as demonstrated by isothermal titration calorimetry. Then, anionic liposomes were coated with -(2-hydroxy) propyl-3-trimethyl ammonium chitosan chloride. The coating involved a size increment from 146.9±2.7 to 194±6.1 nm, for uncoated and coated liposomes, respectively. The ζ-potential was similarly increased from -0.3±1.2 mV to 6±0.4 mV before and after coating, respectively. Chitosan provided mucoadhesion, with an increase in mucin adsorption of 22.9%. Enhancement of permeation through the Calu3 epithelial monolayer was also observed with 10.8% of ghrelin recovered in the basal compartment in comparison to 0% for ghrelin alone. Finally, aerosols generated from two nasal devices (VP3 and SP270) intended for aqueous dispersion were characterized with either coated or uncoated liposomes. Contrarily to the SP270 device, VP3 device showed minor changes between coated and uncoated liposome aerosols, as shown by their median volume diameters of 38.4±5.76 and 37.6±5.74 µm, respectively. Overall, the results obtained in this study show that the developed formulation delivered by the VP3 device can be considered as a potential candidate for nose-brain delivery of ghrelin.
[Mh] Termos MeSH primário: Caquexia/tratamento farmacológico
Sistemas de Liberação de Medicamentos/métodos
Grelina/administração & dosagem
Lipossomos/administração & dosagem
Lipossomos/química
[Mh] Termos MeSH secundário: Administração Intranasal/instrumentação
Adsorção
Aerossóis/química
Encéfalo/efeitos dos fármacos
Quitosana/análogos & derivados
Quitosana/química
Estabilidade de Medicamentos
Grelina/química
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Mucinas/metabolismo
Compostos de Amônio Quaternário/química
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Ghrelin); 0 (Liposomes); 0 (Mucins); 0 (N-(2-hydroxypropyl)-3-trimethylammonium chitosan); 0 (Quaternary Ammonium Compounds); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S147650


  3 / 41945 MEDLINE  
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[PMID]:29273570
[Au] Autor:White RM
[Ad] Endereço:Centre for Forensic Sciences, RTI International, Research Triangle Park, North Carolina, United States of America.
[Ti] Título:Instability and poor recovery of cannabinoids in urine, oral fluid, and hair.
[So] Source:Forensic Sci Rev;30(1):33-49, 2018 Jan.
[Is] ISSN:1042-7201
[Cp] País de publicação:China (Republic : 1949- )
[La] Idioma:eng
[Ab] Resumo:Cannabinoids including, but not limited to Δ9-tetrahydrocannabinol, 11-hydroxytetrahydrocannabinol, and (-)-11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid are known to toxicologists and synthetic chemists as difficult compounds because they are subject to numerous degradative pathways. It is the purpose of this short review article to discuss common pathways that result in the disappearance of cannabinoids - such as conjugate formation, adsorption to surfaces, chemical reactions, microbial action, thermal decomposition, chemical bonding, photosensitivity, sample handling, analytical methodology, and micelle trapping - and to point out possible ways to avoid such degradation.
[Mh] Termos MeSH primário: Canabinoides/análise
Cabelo/química
Saliva/química
[Mh] Termos MeSH secundário: Canabinoides/urina
Estabilidade de Medicamentos
Seres Humanos
Detecção do Abuso de Substâncias
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cannabinoids)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


  4 / 41945 MEDLINE  
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[PMID]:27774855
[Au] Autor:Yin S; Xia Y; Jia Q; Hou ZS; Zhang N
[Ad] Endereço:a Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, College of Chemistry, Chemical Engineering and Materials Science , Shandong Normal University , Jinan , China.
[Ti] Título:Preparation and properties of biomedical segmented polyurethanes based on poly(ether ester) and uniform-size diurethane diisocyanates.
[So] Source:J Biomater Sci Polym Ed;28(1):119-138, 2017 01.
[Is] ISSN:1568-5624
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study describes the preparation and properties of a novel aliphatic cost-effective segmented polyurethanes (SPUs) based on poly(ether ester) (poly-(ε-caprolactone-co-l-lactide)-poly(ethylene glycol)-poly-(ε-caprolactone-co-l-lactide), PECLA) and uniform-size diurethane diisocyanates (HDI-BDO-HDI). PECLA was synthesized via bulk ring-opening polymerization with poly(ethylene glycol) (PEG) as an initiator and ε-caprolactone, l-lactide as monomers. By chain extension of PECLA diol with HDI-BDO-HDI, three SPUs with different hydrophilic segments content and hard segments content were obtained. The chemical structures of the chain extender, PECLA and SPUs were confirmed by H NMR, C NMR, FT-IR, HR-TOF-MS and GPC. The influences of PEG content and uniform-size hard segments on in vitro degradability and mechanical properties of SPU films were researched. Similar thermostability observed in TGA curves of SPU films indicated that the hard segments and PEG content had little influence on the thermostability. The formation of microsphase-separated morphologies, which were demonstrated by the results of DSC and XRD, and physical-linking (H-bonds) network structures led to better mechanical properties of SPU films (ultimate stress: 23.1-17.9 MPa; elongation at break: 840-1130%). The results of water absorption and water contact angle showed that the bulk and surface hydrophilicity were closely related with the hydrophilic PEG content in SPU backbone. And the water absorption being less than 10 wt% indicated that the SPU films had low swelling property. In vitro hydrolytic degradation studies showed that the time of the SPU films becoming fragments was 34-19 days and the degradation rate increased with the increasing content of hydrophilic segments in SPUs, indicating that the degradation rate of SPU films could be controlled by adjusting PEG content. Cytotoxicity test of film extracts were conducted using L929 cells, and the relative growth rate exceeded 90% after incubation for 24, 48 and 72 h, showing excellent cytocompatibility. The acceptable mechanical properties, controllable biodegradability and excellent cytocompatibility of the polyurethanes can make them good candidates for further biomedical applications.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Éter/química
Poliésteres/química
Polietilenoglicóis/química
Poliuretanos/química
[Mh] Termos MeSH secundário: Adsorção
Animais
Materiais Biocompatíveis/toxicidade
Bovinos
Linhagem Celular
Estabilidade de Medicamentos
Fenômenos Mecânicos
Camundongos
Soroalbumina Bovina/química
Temperatura Ambiente
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polyesters); 0 (Polyurethanes); 0 (poly(epsilon-caprolactone-co-lactide)-poly(ethylene glycol)); 059QF0KO0R (Water); 0F5N573A2Y (Ether); 27432CM55Q (Serum Albumin, Bovine); 30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE


  5 / 41945 MEDLINE  
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[PMID]:29406033
[Au] Autor:Alsenedi KA; Morrison C
[Ad] Endereço:Forensic Medicine and Science, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Electronic address: khalid.alsenedi@formed.gla.ac.uk.
[Ti] Título:Determination and long-term stability of twenty-nine cathinones and amphetamine-type stimulants (ATS) in urine using gas chromatography-mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:91-102, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A method was developed for the screening and quantification of seven amphetamine-type stimulants (ATS) and 22 cathinones, including three metabolites, in urine with Gas Chromatography-Mass Spectrometry. This method allowed the detection and quantification of ATS and cathinones group molecules using one procedure. A study of the stability of the drug mixtures for a period of 201 days in human urine samples under three different conditions has been carried. The ATS and cathinones include amphetamine, methamphetamine, MDA, MDEA, MDMA, PMA, PMMA, cathinone, methcathinone, 3'-position-substituted, ring-substituted, methylenedioxy-substituted, N-alkyl-substituted and pyrrolidinyl-substituted. Twenty drugs out of twenty-nine were validated with a quantitative method. This method can be applied to the nine remaining drugs as a screening method. The linearity of the assay was from 50 to 2000 ng/ml, with limits of detection of 0.5 to 10 ng/ml. In terms of accuracy, between-run and within-run precision were ≤20% for 20 compounds with good selectivity. No carryover was seen, and the recovery was between 80 and 120% for most drugs tested. ATS and pyrrolidinyl-substituted groups were conducted to be stable compounds under all conditions. All compounds tested were stable at -20 °C. Some cathinones were primarily degraded after 21 days at 4 °C. They were detectable but unstable after 201 days at 4 °C. Most cathinones were unstable after a day and completely lost after 28 days at RT.
[Mh] Termos MeSH primário: Alcaloides/urina
Anfetamina/urina
Cromatografia Gasosa-Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Alcaloides/química
Anfetamina/química
Estabilidade de Medicamentos
Seres Humanos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 540EI4406J (cathinone); CK833KGX7E (Amphetamine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  6 / 41945 MEDLINE  
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[PMID]:29406031
[Au] Autor:Hofstetter R; Fassauer GM; Link A
[Ad] Endereço:Institute of Pharmacy, Pharmaceutical and Medicinal Chemistry, University of Greifswald, Greifswald, Germany.
[Ti] Título:Supercritical fluid extraction (SFE) of ketamine metabolites from dried urine and on-line quantification by supercritical fluid chromatography and single mass detection (on-line SFE-SFC-MS).
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:77-83, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:On-line solid-phase supercritical fluid extraction (SFE) and chromatography (SFC) coupled to mass spectrometry (MS) has been evaluated for its usefulness with respect to metabolic profiling and pharmacological investigations of ketamine in humans. The aim of this study was to develop and validate a rapid, highly selective and sensitive SFE-SFC-MS method for the quantification of ketamine and its metabolites in miniature amounts in human urine excluding liquid-liquid extraction (LLE). Several conditions were optimized systematically following the requirements of the European Medicines Agency: selectivity, carry-over, calibration curve parameters (LLOQ, range and linearity), within- and between-run accuracy and precision, dilution integrity, matrix effect, and stability. The method, which required a relatively small volume of human urine (20 µL per sample), was validated for pharmacologically and toxicologically relevant concentrations ranging from 25.0 to 1000 ng/mL (r > 0.995). The lower limit of quantification (LLOQ) for all compounds was found to be as low as 0.5 ng. In addition, stability of analytes during removal of water from the urine samples using different conditions (filter paper or ISOLUTE® HM-N) was studied. In conclusion, the method developed in this study can be successfully applied to studies of ketamine metabolites in humans, and may pave the way for routine application of on-line SFE-SFC-MS in clinical investigations.
[Mh] Termos MeSH primário: Cromatografia com Fluido Supercrítico/métodos
Ketamina/urina
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Estabilidade de Medicamentos
Temperatura Alta
Seres Humanos
Ketamina/química
Ketamina/metabolismo
Modelos Lineares
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
690G0D6V8H (Ketamine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  7 / 41945 MEDLINE  
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[PMID]:29406028
[Au] Autor:Yang K; Long XM; Liu YC; Chen FH; Liu XF; Sun ZL; Liu ZY
[Ad] Endereço:Hunan Engineering Research Center of Veterinary Drug, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China.
[Ti] Título:Development and in-house validation of a sensitive LC-MS/MS method for simultaneous quantification of gelsemine, koumine and humantenmine in porcine plasma.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:54-60, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three monomers of G. elegans indole alkaloids (gelsemine, koumine and humantenmine) were simultaneously detected in porcine plasma for the first time with the development and validation of a sensitive and reliable LC-ESI-MS/MS method. Using a gradient mobile phase at a constant flow rate of 0.2 mL/min via electrospray ionization (positive ion mode) in a multiple reaction monitoring (MRM) scan, gelsemine, koumine and humantenmine were eluted, separated and detected at an appropriate retention time. The porcine plasma was prepared using protein precipitation with 1% formic acid-acetonitrile: methanol (2:1, v/v). Using matrix-matched calibration curves and weighted least squares linear regression, a good linearity (r > 0.99) was achieved with a concentration range of 0.1-200 µg/L for gelsemine, koumine and humantenmine; estimated LOD and LOQ values were 0.10 µg/L and 0.2 µg/L, respectively. The mean of the recoveries was in the range of 82.68-100.35% of porcine plasma at four different levels, and the intra-day and inter-day precision (CV) were lower than 15% with a range of 2.46-8.76% and 2.73-10.83%, respectively. The proposed method has proved to be suitable for accurate, quantitative determination of gelsemine, koumine and humantenmine in porcine plasma.
[Mh] Termos MeSH primário: Alcaloides/sangue
Cromatografia Líquida/métodos
Alcaloides de Indol/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Estabilidade de Medicamentos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Indole Alkaloids); 0 (koumine); 5Y13A78Z72 (gelsemine); 82354-38-9 (humantenmine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  8 / 41945 MEDLINE  
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[PMID]:29318304
[Au] Autor:Nikoofard H; Sargolzaei M; Faridbod F
[Ti] Título:Prediction of Physico-chemical Properties of Bacteriostatic N1-Substituted Sulfonamides: Theoretical and Experimental Studies.
[So] Source:Acta Chim Slov;64(4):842-848, 2017 Dec.
[Is] ISSN:1318-0207
[Cp] País de publicação:Slovenia
[La] Idioma:eng
[Ab] Resumo:A computational study at the density functional theory (DFT) as well as electrochemical methods, was carried out on the structural and physico-chemical properties of a series of sulfonamide derivatives (SAs) as WHO essential medications in the treatment of basic health system. The B3LYP/6-311++G(d,p) level of theory carried out on sulfadiazine (SDZ), sulfathiazole (STZ), sulfaquinoxaline (SQX), sulfacetamide (SAA), and the reference unsubstituted sulfonamide (SA) was discussed and rationalized in term of the N1-sulfonamide substituent. The geometric structures and the electronic properties related to the bacteriostatic reactivity were revealed to be affected by the steric and "push-pull" characteristics of the substituents. Electrochemical experiments on oxidation of SAs, using cyclic voltammetry are presented. The results obtained showed that the calculated ionization potentials (IPs) could be correlated linearly with the electro-oxidation potentials. From the molecules studied it is evident that SDZ act as the most electro-active agent, possessing the highest biological activity. DFT computations carried out using the standard molar enthalpies of formation in the gas phase predicted improvements in the thermodynamic stabilization of the SDZ, SQX, and SAA molecules and an unstabilization of STZ with respect to the parent molecule SA.
[Mh] Termos MeSH primário: Antibacterianos/química
Sulfonamidas/química
[Mh] Termos MeSH secundário: Estabilidade de Medicamentos
Eletroquímica
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Sulfonamides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


  9 / 41945 MEDLINE  
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[PMID]:29413579
[Au] Autor:Hegstad S; Havnen H; Helland A; Spigset O; Frost J
[Ad] Endereço:Department of Clinical Pharmacology, St. Olav University Hospital, Trondheim, Norway. Electronic address: solfrid.hegstad@stolav.no.
[Ti] Título:Enantiomeric separation and quantification of R/S-amphetamine in urine by ultra-high performance supercritical fluid chromatography tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1077-1078:7-12, 2018 Mar 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To distinguish between legal and illegal consumption of amphetamine reliable analytical methods for chiral separation of the R- and S-enantiomers of amphetamine in biological specimens are required. In this regard, supercritical fluid chromatography (SFC) has several potential advantages over liquid chromatography, including rapid separation of enantiomers due to low viscosity and high diffusivity of supercritical carbon dioxide, the main component in the SFC mobile phase. A method for enantiomeric separation and quantification of R- and S-amphetamine in urine was developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS). Sample preparation prior to UHPSFC-MS/MS analysis was a semi-automatic solid phase extraction method. The UHPSFC-MS/MS method used a Chiralpak AD-3 column with a mobile phase consisting of CO and 0.2% cyclohexylamine in 2-propanol. The injection volume was 2 µL and run-time was 6 min. MS/MS detection was performed with positive electrospray ionization and two multiple reaction monitoring transitions (m/z 136.1 > 119.0 and m/z 136.1 > 91.0). The calibration range was 50-10,000 ng/mL for each enantiomer. The between-assay relative standard deviations were in the range of 3.7-7.6%. Recovery was 92-93% and matrix effects ranged from 100 to 104% corrected with internal standard. After development and validation, the method has been successfully implemented in routine use at our laboratory for both separation and quantification of R/S-amphetamine, and has proved to be a reliable and useful tool for distinguishing intake of R- and S-amphetamine in authentic patient samples.
[Mh] Termos MeSH primário: Anfetamina/química
Anfetamina/urina
Cromatografia Líquida de Alta Pressão/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estabilidade de Medicamentos
Feminino
Seres Humanos
Limite de Detecção
Modelos Lineares
Masculino
Meia-Idade
Reprodutibilidade dos Testes
Estereoisomerismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
CK833KGX7E (Amphetamine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


  10 / 41945 MEDLINE  
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[PMID]:29413574
[Au] Autor:Gao H; Yu X; Sun R; Yang N; He J; Tao M; Gu H; Yan C; Aa J; Wang G
[Ad] Endereço:Laboratory of Metabolomics, Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
[Ti] Título:Quantitative GC-MS assay of citric acid from humans and db/db mice blood serum to assist the diagnosis of diabetic nephropathy.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1077-1078:28-34, 2018 Mar 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The early diagnosis of diabetic nephropathy (DN) is rather challenging. Our previous study suggested that citric acid is a potential marker for the early diagnosis of diabetic nephropathy in db/db mice. For the first time, in this study, a surrogate analyte of C -citric acid was employed to generate calibration curves for the quantitative measurement of the endogenous citric acid in the sera of db/db mice and diabetic nephropathy patients by GC/MS after the analytes were extracted, methoximated and trimethylsilylated. The constant response factor of C -citric acid versus citric acid over the linear range indicated the identical ionization efficiency of these two compounds. The full validation assessments suggested that the method is sensitive, specific, reliable, reproducible and has acceptable parameters. Statistical analysis revealed cut-off citric acid concentrations of 29.24 µg/mL with a 95% confidence interval between 32.75 and 39.16 µg/mL in the diabetic nephropathy patients and 16.74 and 22.57 µg/mL in the normal controls. The areas under the receiver operating characteristic curves indicated accuracies of over 90% for the diagnoses of early diabetic nephropathy in both humans and db/db mice, which suggests that the serum citric acid level is potentially a biomarker that could assist in the diagnosis of diabetic nephropathy.
[Mh] Termos MeSH primário: Ácido Cítrico/sangue
Nefropatias Diabéticas/metabolismo
Cromatografia Gasosa-Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Animais
Nefropatias Diabéticas/diagnóstico
Estabilidade de Medicamentos
Seres Humanos
Modelos Lineares
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
2968PHW8QP (Citric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE



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