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  1 / 1020 MEDLINE  
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[PMID]:29262451
[Au] Autor:Chen XQ; Ma Q; Zhou LY; Ma HA; Wu JY; Zhao JJ; Yan DN
[Ad] Endereço:Department of Otorhinolaryngology, Affiliated Hospital of Nanjing University of TCM, Nanjing 210029, China.
[Ti] Título:[Experimental study on the effect of Yiqi Wenyang Decoction on nasal mucosa infiltration of NK cells in mice with allergic rhinitis].
[So] Source:Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi;52(12):921-926, 2017 Dec 07.
[Is] ISSN:1673-0860
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To observe the effect of Yiqi Wenyang Decoction on the infiltration and activation of NK cells in nasal mucosa of mouse model with allergic rhinitis (AR), and to explore the potential mechanism for effective intervention of AR with Yiqi Wenyang Decoction. Fourty-eight mice were randomly divided into blank group, model group, low, medium and high dose of Yiqi Wenyang Decoction group and Cetirizine group, with 8 rats in each group. After modeling of AR, the model group was filled with 0.9% sodium chloride solution. Yiqi Wenyang Decoction groups of each dose were given different concentrations of Yiqi Wenyang Decoction water extract, while the Cetirizine group was given aqueous solution of Cetirizine. The behavior, morphological changes of nasal mucosa and infiltration of NK cells in nasal mucosa were observed. The levels of IL-4 and INF-γ in nasal lavage fluid were measured. Besides, the drug safety was observed by acute toxicity test. In the respect of behavioral scoring, middle and high dose of Yiqi Wenyang Decoction group were superior to the model group (number of sneezing: value was 7.189, 8.748, respectively; number of scratching nose: value was 12.074, 14.560, respectively; all <0.05). In middle and high dose of Yiqi Wenyang Decoction group, the infiltration of NK cells and nasal lavage fluid IL-4 levels were lower than those in model group (IOD: value was 10.073, 12.322, respectively; IOD/Area: value was 10.954, 14.073, respectively; IL-4: value was 4.705, 6.801, respectively; all <0.05). There was no significant difference in nasal lavage fluid of INF-γ among each group ( =1.166, >0.05). In acute toxicity test, no obvious poisoning symptoms and death occurred in mice. Yiqi Wenyang Decoction can control the nasal symptom, reduce the local NK cell infiltration of nasal mucosa and inhibit the expression of the 2-type cytokines released by NK cells, which may be related with the potential mechanism of effective intervention of AR with Yiqi Wenyang Decoction.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/uso terapêutico
Células Matadoras Naturais/efeitos dos fármacos
Rinite Alérgica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antialérgicos/efeitos adversos
Antialérgicos/uso terapêutico
Cetirizina/efeitos adversos
Cetirizina/uso terapêutico
Modelos Animais de Doenças
Medicamentos de Ervas Chinesas/efeitos adversos
Interferon gama/análise
Interleucina-4/análise
Camundongos
Líquido da Lavagem Nasal/química
Mucosa Nasal/citologia
Mucosa Nasal/imunologia
Distribuição Aleatória
Rinite Alérgica/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Allergic Agents); 0 (Drugs, Chinese Herbal); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma); YO7261ME24 (Cetirizine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1673-0860.2017.12.009


  2 / 1020 MEDLINE  
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[PMID]:28898981
[Au] Autor:Peterson LR; Woods CW; Davis TE; Wang ZX; Young SA; Osiecki JC; Lewinski MA; Liesenfeld O
[Ad] Endereço:Department of Laboratory Medicine and Pathology, NorthShore University HealthSystem, Evanston, IL.
[Ti] Título:Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus From Nasal Swabs.
[So] Source:Am J Clin Pathol;148(2):119-127, 2017 Aug 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Health care-associated methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (SA) infections are continuing problems. Rapidly determining the MRSA colonization status of a patient facilitates practice to reduce spread of MRSA clinical disease. Sensitive detection of all SA prior to surgery, followed by decolonization, can significantly reduce postoperative infection from this pathogen. Our goal was to validate a new automated assay for this testing. Methods: We compared performance of the cobas MRSA/SA Test on the cobas 4800 System to direct and enriched chromogenic culture using nasal swabs collected from patients at six United States sites. Results: Compared to direct and enriched culture, the sensitivity for MRSA and SA was 93.1% and 93.9%, and the specificity was 97.5% and 94.2%, respectively. After discrepancy analysis, the sensitivity for MRSA and SA was 97.1% and 98.6%, and the specificity was 98.3% and 95.5%, respectively. Compared to direct culture, sensitivity for detecting any SA was 99.6%. Conclusions: The cobas MRSA/SA Test is an effective tool to simultaneously perform surveillance testing for nasal colonization of both MRSA and MSSA.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Cavidade Nasal/microbiologia
Infecções Estafilocócicas/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
DNA Bacteriano/análise
DNA Bacteriano/isolamento & purificação
Feminino
Seres Humanos
Masculino
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Meia-Idade
Líquido da Lavagem Nasal/microbiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqx040


  3 / 1020 MEDLINE  
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[PMID]:28731413
[Au] Autor:Di Cara G; Panfili E; Marseglia GL; Pacitto A; Salvatori C; Testa I; Fabiano C; Verrotti A; Latini A
[Ad] Endereço:Università degli Studi di Perugia, Dipartimento di Scienze Chirurgiche e Biomediche, Perugia, Italy.
[Ti] Título:Association Between Pollen Exposure and Nasal Cytokines in Grass Pollen-Allergic Children.
[So] Source:J Investig Allergol Clin Immunol;27(4):261-263, 2017.
[Is] ISSN:1018-9068
[Cp] País de publicação:Spain
[La] Idioma:eng
[Mh] Termos MeSH primário: Alérgenos/imunologia
Citocinas/imunologia
Exposição Ambiental
Proteína Catiônica de Eosinófilo/imunologia
Poaceae/imunologia
Pólen/imunologia
Rinite Alérgica Sazonal/imunologia
Triptases/imunologia
[Mh] Termos MeSH secundário: Criança
Eosinófilos/imunologia
Feminino
Seres Humanos
Masculino
Mastócitos/imunologia
Líquido da Lavagem Nasal/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Cytokines); EC 3.1.27.- (Eosinophil Cationic Protein); EC 3.1.27.- (RNASE3 protein, human); EC 3.4.21.59 (Tryptases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.18176/jiaci.0161


  4 / 1020 MEDLINE  
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[PMID]:28714988
[Au] Autor:Allen EK; Randolph AG; Bhangale T; Dogra P; Ohlson M; Oshansky CM; Zamora AE; Shannon JP; Finkelstein D; Dressen A; DeVincenzo J; Caniza M; Youngblood B; Rosenberger CM; Thomas PG
[Ad] Endereço:Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
[Ti] Título:SNP-mediated disruption of CTCF binding at the IFITM3 promoter is associated with risk of severe influenza in humans.
[So] Source:Nat Med;23(8):975-983, 2017 Aug.
[Is] ISSN:1546-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies have reported associations of IFITM3 SNP rs12252 with severe influenza, but evidence of association and the mechanism by which risk is conferred remain controversial. We prioritized SNPs in IFITM3 on the basis of putative biological function and identified rs34481144 in the 5' UTR. We found evidence of a new association of rs34481144 with severe influenza in three influenza-infected cohorts characterized by different levels of influenza illness severity. We determined a role for rs34481144 as an expression quantitative trait locus (eQTL) for IFITM3, with the risk allele associated with lower mRNA expression. The risk allele was found to have decreased IRF3 binding and increased CTCF binding in promoter-binding assays, and risk allele carriage diminished transcriptional correlations among IFITM3-neighboring genes, indicative of CTCF boundary activity. Furthermore, the risk allele disrupts a CpG site that undergoes differential methylation in CD8 T cell subsets. Carriers of the risk allele had reduced numbers of CD8 T cells in their airways during natural influenza infection, consistent with IFITM3 promoting accumulation of CD8 T cells in airways and indicating that a critical function for IFITM3 may be to promote immune cell persistence at mucosal sites.Our study identifies a new regulator of IFITM3 expression that associates with CD8 T cell levels in the airways and a spectrum of clinical outcomes.
[Mh] Termos MeSH primário: Influenza Humana/genética
Fator Regulador 3 de Interferon/metabolismo
Proteínas de Membrana/genética
Regiões Promotoras Genéticas/genética
Proteínas de Ligação a RNA/genética
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Alelos
Western Blotting
Fator de Ligação a CCCTC
Linfócitos T CD8-Positivos/imunologia
Metilação de DNA
Predisposição Genética para Doença
Genótipo
Seres Humanos
Influenza Humana/imunologia
Proteínas de Membrana/imunologia
Líquido da Lavagem Nasal/citologia
Polimorfismo de Nucleotídeo Único
Locos de Características Quantitativas
Proteínas de Ligação a RNA/imunologia
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCCTC-Binding Factor); 0 (CTCF protein, human); 0 (IFITM3 protein, human); 0 (IRF3 protein, human); 0 (Interferon Regulatory Factor-3); 0 (Membrane Proteins); 0 (RNA-Binding Proteins); 0 (Repressor Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1038/nm.4370


  5 / 1020 MEDLINE  
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[PMID]:28647455
[Au] Autor:de Silva TI; Gould V; Mohammed NI; Cope A; Meijer A; Zutt I; Reimerink J; Kampmann B; Hoschler K; Zambon M; Tregoning JS
[Ad] Endereço:Section of Paediatrics, Imperial College London, St Mary's Campus, London, W2 1PG, UK; Sheffield Teaching Hospitals NHS Foundation Trust, Royal Hallamshire Hospital, Glossop Road, Sheffield, S10 2JF, UK; Vaccines and Immunity Theme, Medical Research Council Unit The Gambia, PO Box 273, Banjul, Gambi
[Ti] Título:Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity.
[So] Source:J Immunol Methods;449:1-6, 2017 Oct.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We need greater understanding of the mechanisms underlying protection against influenza virus to develop more effective vaccines. To do this, we need better, more reproducible methods of sampling the nasal mucosa. The aim of the current study was to compare levels of influenza virus A subtype-specific IgA collected using three different methods of nasal sampling. Samples were collected from healthy adult volunteers before and after LAIV immunization by nasal wash, flocked swabs and Synthetic Absorptive Matrix (SAM) strips. Influenza A virus subtype-specific IgA levels were measured by haemagglutinin binding ELISA or haemagglutinin binding microarray and the functional response was assessed by microneutralization. Nasosorption using SAM strips lead to the recovery of a more concentrated sample of material, with a significantly higher level of total and influenza H1-specific IgA. However, an equivalent percentage of specific IgA was observed with all sampling methods when normalized to the total IgA. Responses measured using a recently developed antibody microarray platform, which allows evaluation of binding to multiple influenza strains simultaneously with small sample volumes, were compared to ELISA. There was a good correlation between ELISA and microarray values. Material recovered from SAM strips was weakly neutralizing when used in an in vitro assay, with a modest correlation between the level of IgA measured by ELISA and neutralization, but a greater correlation between microarray-measured IgA and neutralizing activity. In conclusion we have tested three different methods of nasal sampling and show that flocked swabs and novel SAM strips are appropriate alternatives to traditional nasal washes for assessment of mucosal influenza humoral immunity.
[Mh] Termos MeSH primário: Anticorpos Antivirais/análise
Imunoglobulina A/análise
Vírus da Influenza A/imunologia
Vacinas contra Influenza/imunologia
Mucosa Nasal/imunologia
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Adulto
Anticorpos Antivirais/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Seres Humanos
Imunidade nas Mucosas
Imunoglobulina A/imunologia
Masculino
Líquido da Lavagem Nasal/imunologia
Mucosa Nasal/virologia
Análise Serial de Proteínas
Manejo de Espécimes/instrumentação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Immunoglobulin A); 0 (Influenza Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE


  6 / 1020 MEDLINE  
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[PMID]:28628840
[Au] Autor:Bernard A; Sardella A; Voisin C; Dumont X
[Ad] Endereço:Louvain Centre for Toxicology and Applied Pharmacology, Institute of Experimental and Clinical Research (IREC), Catholic University of Louvain, Brussels, Belgium.
[Ti] Título:Nasal epithelium injury by chlorination products and other stressors predicts persistent sensitization to aeroallergens in young schoolchildren.
[So] Source:Environ Res;158:145-152, 2017 10.
[Is] ISSN:1096-0953
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Allergic sensitization during childhood is a dynamic process with a substantial rate of remission. Factors influencing this process are largely unknown. METHODS: We conducted a two-year prospective study among 121 schoolchildren (mean age, 5.8 years; 64 boys). We measured urea, club cell protein (CC16), ß -microglobulin and albumin in nasal lavage fluid (NALF) and IgE to cat, pollen or house dust mite (HDM) in nasal mucosa fluid. RESULTS: Odds of persistent sensitization to any aeroallergen increased across baseline ascending tertiles of urea-adjusted ß -microglobulin or albumin and descending tertiles of albumin- or ß -microglobulin-adjusted CC16 (P-trend = 0.006, 0.02, 0.044 and 0.006, respectively). Persistent HDM sensitization also increased with baseline descending tertiles of raw or urea-adjusted CC16 (both P-trend = 0.007). Such strong associations were not observed with new-onset or remitted sensitization to any aeroallergen or with raw NALF concentrations of urea, albumin or ß -microglobulin. At baseline, house cleaning with bleach and chlorinated pool attendance emerged among the strongest and most consistent determinants of NALF biomarkers, being both associated with higher urea and lower CC16 in NALF. CONCLUSION: In young children, a defective nasal epithelium attributable to immaturity or stressors such as chlorination products is predictive of more persistent aeroallergen sensitization.
[Mh] Termos MeSH primário: Alérgenos/toxicidade
Compostos Clorados/toxicidade
Líquido da Lavagem Nasal/química
Mucosa Nasal/efeitos dos fármacos
[Mh] Termos MeSH secundário: Albuminas/metabolismo
Bélgica
Pré-Escolar
Feminino
Seres Humanos
Imunoglobulina E/metabolismo
Masculino
Mucosa Nasal/lesões
Estudos Prospectivos
Fatores de Risco
Uteroglobina/metabolismo
Microglobulina-2 beta/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Albumins); 0 (Allergens); 0 (Chlorine Compounds); 0 (SCGB1A1 protein, human); 0 (beta 2-Microglobulin); 37341-29-0 (Immunoglobulin E); 9060-09-7 (Uteroglobin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


  7 / 1020 MEDLINE  
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[PMID]:28284536
[Au] Autor:Badorrek P; Müller M; Koch W; Hohlfeld JM; Krug N
[Ad] Endereço:Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany. Electronic address: philipp.badorrek@item.fraunhofer.de.
[Ti] Título:Specificity and reproducibility of nasal biomarkers in patients with allergic rhinitis after allergen challenge chamber exposure.
[So] Source:Ann Allergy Asthma Immunol;118(3):290-297, 2017 Mar.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Allergic rhinitis is an inflammatory disease that causes cellular influx and mediator release in the nose. These inflammatory changes might be used as nasal biomarkers to assess the efficacy of novel anti-allergic treatments. OBJECTIVE: To assess the specificity and reproducibility of nasal biomarkers in patients with allergic rhinitis after grass pollen exposure in an allergen challenge chamber. METHODS: In a monocenter pilot study, 15 patients with allergic rhinitis and 19 healthy individuals underwent two 4-hour Dactylis glomerate pollen challenges in the challenge chamber with an interval of 21 days. Before challenge, on exit, and after 2 and 22 hours, a nasal lavage was performed and nasal secretions were collected on filter paper to determine a wide panel of cells and mediators. Furthermore, total nasal symptom score, nasal flow, and nasal nitric oxide were measured. RESULTS: Pollen exposure significantly increased eosinophil, interleukin (IL) 5, IL-6, IL-13, and macrophage inflammatory protein 1ß levels in allergic patients but not in healthy individuals. The effect could be reproduced for eosinophils, IL-5, IL-6, and macrophage inflammatory protein 1ß after the second allergen challenge. By contrast, the IL-13 levels were higher and eotaxin levels first increased after repetitive allergen challenge. There was no correlation between total nasal symptom score and elevated cell or cytokine levels. Nasal nitric oxide levels were nonspecifically elevated in both patients with allergy and healthy controls. CONCLUSION: A subset of cellular and soluble biomarkers in nasal lavage and secretion reveals specificity and reproducibility in patients with allergic rhinitis. These can be used to measure the immunologic efficacy of antiallergic treatments in an allergen challenge chamber. Carryover effects attributable to priming must be considered when designing cross-over studies. TRIAL REGISTRATION: clinicaltrials.gov Identifier: NCT00297843.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Biomarcadores
Mucosa Nasal/imunologia
Mucosa Nasal/metabolismo
Rinite Alérgica/imunologia
Rinite Alérgica/metabolismo
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Citocinas/metabolismo
Feminino
Seres Humanos
Imunização
Mediadores da Inflamação/metabolismo
Contagem de Leucócitos
Masculino
Líquido da Lavagem Nasal/imunologia
Testes de Provocação Nasal
Óxido Nítrico/metabolismo
Projetos Piloto
Pólen/imunologia
Rinite Alérgica/diagnóstico
Rinomanometria
Índice de Gravidade de Doença
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Biomarkers); 0 (Cytokines); 0 (Inflammation Mediators); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  8 / 1020 MEDLINE  
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[PMID]:28238741
[Au] Autor:Takahashi T; Kato A; Berdnikovs S; Stevens WW; Suh LA; Norton JE; Carter RG; Harris KE; Peters AT; Hulse KE; Grammer LC; Welch KC; Shintani-Smith S; Tan BK; Conley DB; Kern RC; Bochner BS; Schleimer RP
[Ad] Endereço:Division of Allergy-Immunology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Ill.
[Ti] Título:Microparticles in nasal lavage fluids in chronic rhinosinusitis: Potential biomarkers for diagnosis of aspirin-exacerbated respiratory disease.
[So] Source:J Allergy Clin Immunol;140(3):720-729, 2017 Sep.
[Is] ISSN:1097-6825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Microparticles (MPs) are submicron-sized shed membrane vesicles released from activated or injured cells and are detectable by flow cytometry. MP levels have been used as biomarkers to evaluate cell injury or activation in patients with pathological conditions. OBJECTIVE: We sought to compare MP types and levels in nasal lavage fluids (NLFs) from controls and patients with chronic rhinosinusitis without nasal polyps (CRSsNP), chronic rhinosinusitis with nasal polyps (CRSwNP), and aspirin-exacerbated respiratory disease (AERD). METHODS: We collected NLFs from patients with CRSsNP (n = 33), CRSwNP (n = 45), and AERD (n = 31) and control (n = 24) subjects. Standardized flow cytometry methods were used to characterize the following MP types: endothelial MPs, epithelial MPs (epithelial cell adhesion molecule [EpCAM](+)MPs, E-cadherin(+)MPs), platelet MPs (CD31(+)CD41(+)MPs), eosinophil MPs (EGF-like module-containing mucin-like hormone receptor-like 1[EMR1](+)MPs), mast cell MPs (high-affinity IgE receptor [FcεRI](+)c-kit(+)MPs), and basophil MPs (CD203c(+)c-kit(-)MPs). Basophil activation was evaluated by the mean fluorescence intensity of CD203c on basophil MPs. RESULTS: Activated mast cell MPs (CD137(+) FcεRI(+)c-kit(+)MPs) were significantly increased in NLFs of controls compared with NLFs of patients with CRSsNP (2.3-fold; P < .02), CRSwNP (2.3-fold; P < .03), and AERD (7.4-fold; P < .0001). Platelet MPs (3.5-fold; P < .01) and basophil MPs (2.5-fold; P < .05) were increased only in patients with AERD. Mean fluorescence intensity of CD203c on MPs was increased in patients with CRSwNP (P < .002) and AERD (P < .0001), but not in patients with CRSsNP. EpCAM(+)MPs in patients with CRSwNP were no different from control (P = .91) and lower than those in patients with CRSsNP (P < .02) and AERD (P < .002). CONCLUSIONS: Based on released MPs, mast cells, platelets, and basophils were more highly activated in patients with AERD than in patients with CRS. Epithelial injury was lower in patients with CRSwNP than in patients with CRSsNP and AERD. MP analysis may help identify phenotypes of CRS, and in distinguishing AERD from CRSwNP.
[Mh] Termos MeSH primário: Asma Induzida por Aspirina/patologia
Micropartículas Derivadas de Células
Líquido da Lavagem Nasal/citologia
Pólipos Nasais/patologia
Rinite/patologia
Sinusite/patologia
[Mh] Termos MeSH secundário: Adulto
Biomarcadores
Doença Crônica
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE


  9 / 1020 MEDLINE  
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[PMID]:27986411
[Au] Autor:Teran LM; Ramirez-Jimenez F; Soid-Raggi G; Velazquez JR
[Ad] Endereço:Departamento de Inmunogenética y Alergia, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico.
[Ti] Título:Interleukin 16 and CCL17/thymus and activation-regulated chemokine in patients with aspirin-exacerbated respiratory disease.
[So] Source:Ann Allergy Asthma Immunol;118(2):191-196, 2017 Feb.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Interleukin (IL) 16 and thymus and activation-regulated cytokine (TARC) are chemoattractant cytokines for eosinophils and T 2 cells. Differential levels of these components in aspirin-exacerbated respiratory disease (AERD) and allergic rhinitis with asthma (ARwA) may be related to a different inflammatory response in both asthma phenotypes. OBJECTIVE: To assess the nasal lavage immunoreactivity of IL-16 and TARC cytokines. METHODS: We used multienzyme-linked immunosorbent assays to detect IL-5, IL-13, IL-16, IL-33, I-309/CCL1, TARC/CCL17, monocyte-derived chemokine/CCL22, periostin, and eosinophil cationic protein levels in nasal lavages from patients with AERD and patients with ARwA. RESULTS: The IL-13, IL-16, TARC, and periostin levels were significantly higher in patients with AERD compared with those of patients with ARwA. Correlation analysis of mediator levels in AERD revealed a possible role of IL-16 and TARC in eosinophil recruitment and activation. CONCLUSION: IL-16, TARC, and periostin distinguish between patients with AERD and those with ARwA. These mediators, taken together rather than individually, may comprise good specific nasal markers in patients with AERD. The effects of IL-16 and TARC on T 1, T 2, and T-regulatory cell functions in AERD cannot be disregarded.
[Mh] Termos MeSH primário: Aspirina/efeitos adversos
Quimiocina CCL17/metabolismo
Hipersensibilidade a Drogas/etiologia
Hipersensibilidade a Drogas/metabolismo
Interleucina-16/metabolismo
Hipersensibilidade Respiratória/etiologia
Hipersensibilidade Respiratória/metabolismo
[Mh] Termos MeSH secundário: Adulto
Biomarcadores
Hipersensibilidade a Drogas/diagnóstico
Eosinófilos/imunologia
Eosinófilos/metabolismo
Feminino
Seres Humanos
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Masculino
Meia-Idade
Líquido da Lavagem Nasal/imunologia
Fenótipo
Testes de Função Respiratória
Hipersensibilidade Respiratória/diagnóstico
Testes Cutâneos
Células Th2/imunologia
Células Th2/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Chemokine CCL17); 0 (Interleukin-16); 37341-29-0 (Immunoglobulin E); R16CO5Y76E (Aspirin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27717558
[Au] Autor:Min JY; Ocampo CJ; Stevens WW; Price CP; Thompson CF; Homma T; Huang JH; Norton JE; Suh LA; Pothoven KL; Conley DB; Welch KC; Shintani-Smith S; Peters AT; Grammer LC; Harris KE; Hulse KE; Kato A; Modyanov NN; Kern RC; Schleimer RP; Tan BK
[Ad] Endereço:Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, Ill.
[Ti] Título:Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric H,K-ATPase.
[So] Source:J Allergy Clin Immunol;139(1):130-141.e11, 2017 Jan.
[Is] ISSN:1097-6825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is often characterized by tissue eosinophilia that is associated with poor prognosis. Recent findings that proton pump inhibitors (PPIs) directly modulate the expression of eotaxin-3, an eosinophil chemoattractant, in patients with eosinophilic diseases suggest therapeutic potential for PPIs in those with CRSwNP. OBJECTIVE: We assessed the effect of type 2 mediators, particularly IL-13 and eotaxin-3, on tissue eosinophilia and disease severity in patients with chronic rhinosinusitis (CRS). Further investigation focused on PPI suppression of eotaxin-3 expression in vivo and in vitro, with exploration of underlying mechanisms. METHODS: Type 2 mediator levels in nasal tissues and secretions were measured by using a multiplex immunoassay. Eotaxin-3 and other chemokines expressed in IL-13-stimulated human sinonasal epithelial cells (HNECs) and BEAS-2B cells with or without PPIs were assessed by using ELISA, Western blotting, real-time PCR, and intracellular pH imaging. RESULTS: Nasal tissues and secretions from patients with CRSwNP had increased IL-13, eotaxin-2, and eotaxin-3 levels, and these were positively correlated with tissue eosinophil cationic protein levels and radiographic scores in patients with CRS (P < .05). IL-13 stimulation of HNECs and BEAS-2B cells dominantly induced eotaxin-3 expression, which was significantly inhibited by PPIs (P < .05). Patients with CRS taking PPIs also showed lower in vivo eotaxin-3 levels compared with those without PPIs (P < .05). Using intracellular pH imaging and altering extracellular K , we found that IL-13 enhanced H ,K -exchange, which was blocked by PPIs and the mechanistically unrelated H,K-ATPase inhibitor, SCH-28080. Furthermore, knockdown of ATP12A (gene for the nongastric H,K-ATPase) significantly attenuated IL-13-induced eotaxin-3 expression in HNECs. PPIs also had effects on accelerating IL-13-induced eotaxin-3 mRNA decay. CONCLUSION: Our results demonstrated that PPIs reduce IL-13-induced eotaxin-3 expression by airway epithelial cells. Furthermore, mechanistic studies suggest that the nongastric H,K-ATPase is necessary for IL-13-mediated epithelial responses, and its inhibitors, including PPIs, might be of therapeutic value in patients with CRSwNP by reducing epithelial production of eotaxin-3.
[Mh] Termos MeSH primário: Citocinas/imunologia
ATPase Trocadora de Hidrogênio-Potássio/imunologia
Pólipos Nasais/imunologia
Inibidores da Bomba de Prótons/farmacologia
Rinite/imunologia
Sinusite/imunologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Benzimidazóis/farmacologia
Linhagem Celular
Células Cultivadas
Doença Crônica
Citocinas/genética
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/imunologia
Feminino
Técnicas de Silenciamento de Genes
ATPase Trocadora de Hidrogênio-Potássio/genética
ATPase Trocadora de Hidrogênio-Potássio/metabolismo
Seres Humanos
Imidazóis/farmacologia
Masculino
Meia-Idade
Líquido da Lavagem Nasal/imunologia
Mucosa Nasal/citologia
Mucosa Nasal/efeitos dos fármacos
Mucosa Nasal/imunologia
Pólipos Nasais/diagnóstico por imagem
Eosinofilia Pulmonar/diagnóstico por imagem
Eosinofilia Pulmonar/imunologia
Rinite/diagnóstico por imagem
Sinusite/diagnóstico por imagem
Tomografia Computadorizada por Raios X
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzimidazoles); 0 (Cytokines); 0 (Imidazoles); 0 (Proton Pump Inhibitors); 00427X161I (Sch 28080); EC 3.6.3.10 (ATP12A protein, human); EC 3.6.3.10 (H(+)-K(+)-Exchanging ATPase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE



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