Base de dados : MEDLINE
Pesquisa : E07.858.082.212 [Categoria DeCS]
Referências encontradas : 546 [refinar]
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[PMID]:29274337
[Au] Autor:Ueda K; Onishi A; Ito SI; Nakamura M; Takahashi M
[Ad] Endereço:Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan; Division of Ophthalmology, Department of Surgery, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan.
[Ti] Título:Generation of three-dimensional retinal organoids expressing rhodopsin and S- and M-cone opsins from mouse stem cells.
[So] Source:Biochem Biophys Res Commun;495(4):2595-2601, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Three-dimensional retinal organoids can be differentiated from embryonic stem cells/induced pluripotent stem cells (ES/iPS cells) under defined medium conditions. We modified the serum-free floating culture of embryoid body-like aggregates with quick reaggregation (SFEBq) culture procedure to obtain retinal organoids expressing more rod photoreceptors and S- and M-cone opsins. METHODS: Retinal organoids differentiated from mouse Nrl-eGFP iPS cells were cultured in various mediums during photoreceptor development. To promote rod photoreceptor development, organoids were maintained in media containing 9-cis retinoic acids (9cRA). To obtain retinal organoids with M-opsin expression, we cultured in medium with 1% fetal bovine serum (FBS) supplemented with T3, BMP4, and DAPT. Section immunohistochemistry was performed to visualize the expression of photoreceptor markers. RESULTS: In three-dimensional (3D) retinas exposed to 9cRA, rhodopsin was expressed earlier and S-cone opsins were suppressed. We could maintain 3D retinas up to DD 35 in culture media with 1% FBS. The 3D retinas expressed rhodopsin, S- and M-opsins, but most cone photoreceptors expressed either S- or M-opsins. CONCLUSION: By modifying culture conditions in the SFEBq protocol, we obtained rod-dominated 3D retinas and S- and M-opsin expressing 3D retinas.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Opsinas dos Cones/metabolismo
Retina/citologia
Retina/crescimento & desenvolvimento
Rodopsina/metabolismo
Células-Tronco/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/fisiologia
Células Cultivadas
Camundongos
Organogênese/fisiologia
Impressão Tridimensional
Células Fotorreceptoras Retinianas Cones/metabolismo
Células-Tronco/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cone Opsins); 9009-81-8 (Rhodopsin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE


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[PMID]:28471698
[Au] Autor:Akintewe OO; Roberts EG; Rim NG; Ferguson MAH; Wong JY
[Ad] Endereço:Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215; email: olukemi@bu.edu , ngrim@bu.edu , fergu@bu.edu.
[Ti] Título:Design Approaches to Myocardial and Vascular Tissue Engineering.
[So] Source:Annu Rev Biomed Eng;19:389-414, 2017 Jun 21.
[Is] ISSN:1545-4274
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Engineered tissues represent an increasingly promising therapeutic approach for correcting structural defects and promoting tissue regeneration in cardiovascular diseases. One of the challenges associated with this approach has been the necessity for the replacement tissue to promote sufficient vascularization to maintain functionality after implantation. This review highlights a number of promising prevascularization design approaches for introducing vasculature into engineered tissues. Although we focus on encouraging blood vessel formation within myocardial implants, we also discuss techniques developed for other tissues that could eventually become relevant to engineered cardiac tissues. Because the ultimate solution to engineered tissue vascularization will require collaboration between wide-ranging disciplines such as developmental biology, tissue engineering, and computational modeling, we explore contributions from each field.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Vasos Sanguíneos/crescimento & desenvolvimento
Coração/crescimento & desenvolvimento
Dispositivos Lab-On-A-Chip
Técnicas de Cultura de Órgãos/métodos
Engenharia Tecidual/métodos
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Reatores Biológicos
Vasos Sanguíneos/citologia
Células Cultivadas
Seres Humanos
Miocárdio/citologia
Técnicas de Cultura de Órgãos/instrumentação
Engenharia Tecidual/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-bioeng-071516-044641


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[PMID]:28183636
[Au] Autor:Immich APS; Pennacchi PC; Naves AF; Felisbino SL; Boemo RL; Maria-Engler SS; Catalani LH
[Ad] Endereço:Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
[Ti] Título:Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:48-58, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (l-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Miringoplastia
Regeneração
Membrana Timpânica/fisiologia
Membrana Timpânica/cirurgia
[Mh] Termos MeSH secundário: Animais
Morte Celular
Criança
Pré-Escolar
Endoscopia
Seres Humanos
Imuno-Histoquímica
Lactente
Queratinas/metabolismo
Antígeno Ki-67/metabolismo
Masculino
Polímeros/química
Ratos Sprague-Dawley
Temperatura Ambiente
Engenharia Tecidual
Tecidos Suporte/química
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ki-67 Antigen); 0 (Polymers); 68238-35-7 (Keratins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


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[PMID]:28179160
[Au] Autor:Versteegden LR; van Kampen KA; Janke HP; Tiemessen DM; Hoogenkamp HR; Hafmans TG; Roozen EA; Lomme RM; van Goor H; Oosterwijk E; Feitz WF; van Kuppevelt TH; Daamen WF
[Ad] Endereço:Department of Biochemistry, Radboud Institute for Molecular Life Science, Radboud University Medical Center, Geert Grooteplein 26-28, PO Box 9101, 6500 HB Nijmegen, The Netherlands. Electronic address: Luuk.Versteegden@radboudumc.nl.
[Ti] Título:Tubular collagen scaffolds with radial elasticity for hollow organ regeneration.
[So] Source:Acta Biomater;52:1-8, 2017 Apr 01.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tubular collagen scaffolds have been used for the repair of damaged hollow organs in regenerative medicine, but they generally lack the ability to reversibly expand in radial direction, a physiological characteristic seen in many native tubular organs. In this study, tubular collagen scaffolds were prepared that display a shape recovery effect and therefore exhibit radial elasticity. Scaffolds were constructed by compression of fibrillar collagen around a star-shaped mandrel, mimicking folds in a lumen, a typical characteristic of empty tubular hollow organs, such as ureter or urethra. Shape recovery effect was introduced by in situ fixation using a star-shaped mandrel, 3D-printed clamps and cytocompatible carbodiimide crosslinking. Prepared scaffolds expanded upon increase of luminal pressure and closed to the star-shaped conformation after removal of pressure. In this study, we applied this method to construct a scaffold mimicking the dynamics of human urethra. Radial expansion and closure of the scaffold could be iteratively performed for at least 1000 cycles, burst pressure being 132±22mmHg. Scaffolds were seeded with human epithelial cells and cultured in a bioreactor under dynamic conditions mimicking urination (pulse flow of 21s every 2h). Cells adhered and formed a closed luminal layer that resisted flow conditions. In conclusion, a new type of a tubular collagen scaffold has been constructed with radial elastic-like characteristics based on the shape of the scaffold, and enabling the scaffold to reversibly expand upon increase in luminal pressure. These scaffolds may be useful for regenerative medicine of tubular organs. STATEMENT OF SIGNIFICANCE: In this paper, a new type I collagen-based tubular scaffold is presented that possesses intrinsic radial elasticity. This characteristic is key to the functioning of a number of tubular organs including blood vessels and organs of the gastrointestinal and urogenital tract. The scaffold was given a star-shaped lumen by physical compression and chemical crosslinking, mimicking the folding pattern observed in many tubular organs. In rest, the lumen is closed but it opens upon increase of luminal pressure, e.g. when fluids pass. Human epithelial cells seeded on the luminal side adhered well and were compatible with voiding dynamics in a bioreactor. Collagen scaffolds with radial elasticity may be useful in the regeneration of dynamic tubular organs.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Colágeno Tipo I/química
Células Epiteliais/citologia
Regeneração Tecidual Guiada/instrumentação
Técnicas de Cultura de Órgãos/instrumentação
Organogênese/fisiologia
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/química
Proliferação Celular/fisiologia
Células Cultivadas
Células Epiteliais/fisiologia
Desenho de Equipamento
Análise de Falha de Equipamento
Proteínas da Matriz Extracelular/química
Seres Humanos
Teste de Materiais
Impressão Tridimensional
Engenharia Tecidual/instrumentação
Engenharia Tecidual/métodos
Tecidos Suporte
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Collagen Type I); 0 (Extracellular Matrix Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE


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[PMID]:27956361
[Au] Autor:Pflaum M; Kühn-Kauffeldt M; Schmeckebier S; Dipresa D; Chauhan K; Wiegmann B; Haug RJ; Schein J; Haverich A; Korossis S
[Ad] Endereço:Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Hannover Medical School, 30625 Hannover, Germany. Electronic address: pflaum.michael@mh-hannover.de.
[Ti] Título:Endothelialization and characterization of titanium dioxide-coated gas-exchange membranes for application in the bioartificial lung.
[So] Source:Acta Biomater;50:510-521, 2017 Mar 01.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fouling on the gas-exchange hollow-fiber membrane (HFM) of extracorporeal membrane oxygenation (ECMO) devices by blood components and pathogens represents the major hurdle to their long-term application in patients with lung deficiency or unstable hemodynamics. Although patients are treated with anticoagulants, deposition of blood proteins onto the membrane surface may still occur after few days, leading to insufficient gas transfer and, consequently, to device failure. The aim of this study was to establish an endothelial cell (EC) monolayer onto the gas-exchange membrane of an ECMO device with a view to developing a hemocompatible bioartificial lung. Poly(4-methyl-1-pentene) (PMP) gas-exchange membranes were coated with titanium dioxide (TiO ), using the pulsed vacuum cathodic arc plasma deposition (PVCAPD) technique, in order to generate a stable interlayer, enabling cell adhesion onto the strongly hydrophobic PMP membrane. The TiO coating reduced the oxygen transfer rate (OTR) of the membrane by 22%, and it successfully mediated EC attachment. The adhered ECs formed a confluent monolayer, which retained a non-thrombogenic state and showed cell-to-cell, as well as cell-to-substrate contacts. The established monolayer was able to withstand physiological shear stress and possessed a "self-healing" capacity at areas of induced monolayer disruption. The study demonstrated that the TiO coating mediated EC attachment and the establishment of a functional EC monolayer. STATEMENT OF SIGNIFICANCE: Surface endothelialization is considered an effective approach to achieve complete hamocompatibility of blood-contacting devices. Several strategies to enable endothelial cell adhesion onto stents and vascular prostheses have already been described in the literature. However, only few studies investigated the feasibility of establishing an endothelial monolayer onto the gas exchange membrane of ECMO devices, using peptides or proteins that were weakly adsorbed via dip coating techniques. This study demonstrated the effectiveness of an alternative and stable titanium dioxide coating for gas-exchange membranes, which enabled the establishment of a confluent, functional and non-activated endothelial monolayer, while maintaining oxygen permeability.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Materiais Revestidos Biocompatíveis/farmacologia
Células Endoteliais da Veia Umbilical Humana/citologia
Pulmão/efeitos dos fármacos
Membranas Artificiais
Oxigênio/química
Titânio/farmacologia
[Mh] Termos MeSH secundário: Plaquetas/efeitos dos fármacos
Plaquetas/ultraestrutura
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células HL-60
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Seres Humanos
Adesividade Plaquetária/efeitos dos fármacos
Polienos/química
Reação em Cadeia da Polimerase em Tempo Real
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 0 (Membranes, Artificial); 0 (Polyenes); 15FIX9V2JP (titanium dioxide); 25068-26-2 (poly(4-methyl-1-pentene)); D1JT611TNE (Titanium); S88TT14065 (Oxygen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE


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[PMID]:27869295
[Au] Autor:Einstein SA; Weegman BP; Kitzmann JP; Papas KK; Garwood M
[Ad] Endereço:Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota, 2021 Sixth Street SE, Minneapolis, Minnesota, 55455.
[Ti] Título:Noninvasive assessment of tissue-engineered graft viability by oxygen-17 magnetic resonance spectroscopy.
[So] Source:Biotechnol Bioeng;114(5):1118-1121, 2017 May.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transplantation of macroencapsulated tissue-engineered grafts (TEGs) is being investigated as a treatment for type 1 diabetes, but there is a critical need to measure TEG viability both in vitro and in vivo. Oxygen deficiency is the most critical issue preventing widespread implementation of TEG transplantation and delivery of supplemental oxygen (DSO) has been shown to enhance TEG survival and function in vivo. In this study, we demonstrate the first use of oxygen-17 magnetic resonance spectroscopy ( O-MRS) to measure the oxygen consumption rate (OCR) of TEGs and show that in addition to providing therapeutic benefits to TEGs, DSO with O can also enable measurements of TEG viability. Macroencapsulated TEGs containing ßTC3 murine insulinoma cells were prepared with three fractional viabilities and provided with O . Cellular metabolism of O into nascent mitochondrial water (H O) was monitored by O-MRS and, from the measured data, OCR was calculated. For comparison, OCR was simultaneously measured on a separate, but equivalent sample of cells with a well-established stirred microchamber technique. OCR measured by O-MRS agreed well with measurements made in the stirred microchamber device. These studies confirm that O-MRS can quantify TEG viability noninvasively. Biotechnol. Bioeng. 2017;114: 1118-1121. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Sobrevivência de Enxerto/fisiologia
Espectroscopia de Ressonância Magnética/métodos
Isótopos de Oxigênio/metabolismo
Pâncreas Artificial
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Camundongos
Modelos Biológicos
Isótopos de Oxigênio/análise
Engenharia Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oxygen Isotopes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26227


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[PMID]:27600719
[Au] Autor:Martín-Piedra MA; Alaminos M; Fernández-Valadés-Gámez R; España-López A; Liceras-Liceras E; Sánchez-Montesinos I; Martínez-Plaza A; Sánchez-Quevedo MC; Fernández-Valadés R; Garzón I
[Ad] Endereço:Department of Histology (Tissue Engineering Group), University of Granada and research institute ibs.GRANADA, Granada, Spain.
[Ti] Título:Development of a multilayered palate substitute in rabbits: a histochemical ex vivo and in vivo analysis.
[So] Source:Histochem Cell Biol;147(3):377-388, 2017 Mar.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Current tissue engineering technology focuses on developing simple tissues, whereas multilayered structures comprising several tissue types have rarely been described. We developed a highly biomimetic multilayered palate substitute with bone and oral mucosa tissues using rabbit cells and biomaterials subjected to nanotechnological techniques based on plastic compression. This novel palate substitute was autologously grafted in vivo, and histological and histochemical analyses were used to evaluate biointegration, cell function, and cell differentiation in the multilayered palate substitute. The three-dimensional structure of the multilayered palate substitute was histologically similar to control tissues, but the ex vivo level of cell and tissue differentiation were low as determined by the absence of epithelial differentiation although cytokeratins 4 and 13 were expressed. In vivo grafting was associated with greater cell differentiation, epithelial stratification, and maturation, but the expression of cytokeratins 4, 13, 5, and 19 at did not reach control tissue levels. Histochemical analysis of the oral mucosa stroma and bone detected weak signals for proteoglycans, elastic and collagen fibers, mineralization deposits and osteocalcin in the multilayered palate substitute cultured ex vivo. However, in vivo grafting was able to induce cell and tissue differentiation, although the expression levels of these components were always significantly lower than those found in controls, except for collagen in the bone layer. These results suggest that generation of a full-thickness multilayered palate substitute is achievable and that tissues become partially differentiated upon in vivo grafting.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Materiais Biocompatíveis
Palato/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Osso e Ossos/citologia
Diferenciação Celular
Células Cultivadas
Técnicas In Vitro
Mucosa Bucal/citologia
Mucosa Bucal/transplante
Palato/anatomia & histologia
Coelhos
Transplante Autólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-016-1489-5


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[PMID]:27418522
[Au] Autor:Salehi-Nik N; Banikarimi SP; Amoabediny G; Pouran B; Shokrgozar MA; Zandieh-Doulabi B; Klein-Nulend J
[Ad] Endereço:School of Chemical Engineering, College of Engineering, University of Tehran, Tehran.
[Ti] Título:Flow Preconditioning of Endothelial Cells on Collagen-Immobilized Silicone Fibers Enhances Cell Retention and Antithrombotic Function.
[So] Source:Artif Organs;41(6):556-567, 2017 Jun.
[Is] ISSN:1525-1594
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stability and antithrombotic functionality of endothelial cells on silicone hollow fibers (SiHFs) are critical in the development of biohybrid artificial lungs. Here we aimed to enhance endothelial cell retention and anti-thrombotic function by low (12 dyn/cm , 24 h) fluid shear stress ("flow") preconditioning of endothelial cells seeded on collagen-immobilized SiHFs. The response of endothelial cells without preconditioning (48 h static culture) and with preconditioning (24 h static culture followed by 24 h flow preconditioning) on hollow fibers to high fluid shear stress (30 dyn/cm , 1 h) was assessed in a parallel-plate flow chamber. Finite element (FE) modeling was used to simulate shear stress within the flow chamber. We found that collagen immobilization on hollow fibers using carbodiimide bonds provided sufficient stability to high shear stress. Flow preconditioning for 24 h before treatment with high shear stress for 1 h on collagen-immobilized hollow fibers increased cell retention (1.3-fold). The FE model showed that cell flattening due to flow preconditioning reduced maximum shear stress on cells by 32%. Flow preconditioning prior to exposure to high fluid shear stress enhanced the production of nitric oxide (1.3-fold) and prostaglandin I (1.2-fold). In conclusion, flow preconditioning of endothelial cells on collagen-immobilized SiHFs enhanced cell retention and antithrombotic function, which could significantly improve current biohybrid artificial lungs.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Materiais Revestidos Biocompatíveis/química
Colágeno/química
Células Endoteliais/citologia
Silicones/química
Engenharia Tecidual/instrumentação
[Mh] Termos MeSH secundário: Adesão Celular
Células Endoteliais/metabolismo
Endotélio Vascular/citologia
Endotélio Vascular/metabolismo
Desenho de Equipamento
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Hidrodinâmica
Proteínas Imobilizadas/química
Pulmão/irrigação sanguínea
Pulmão/citologia
Pulmão/fisiologia
Teste de Materiais
Óxido Nítrico/metabolismo
Prostaglandinas/metabolismo
Estresse Mecânico
Engenharia Tecidual/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 0 (Immobilized Proteins); 0 (Prostaglandins); 0 (Silicones); 31C4KY9ESH (Nitric Oxide); 9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1111/aor.12759


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[PMID]:27830896
[Au] Autor:Tveito K
[Ti] Título:No shortcut to success.
[Ti] Título:Ingen snarvei til suksess..
[So] Source:Tidsskr Nor Laegeforen;136(20):1699, 2016 Nov.
[Is] ISSN:0807-7096
[Cp] País de publicação:Norway
[La] Idioma:eng; nor
[Mh] Termos MeSH primário: Má Conduta Científica
Experimentação Humana Terapêutica
Traqueia/transplante
[Mh] Termos MeSH secundário: Órgãos Bioartificiais
Seres Humanos
Transplante de Órgãos
Segurança do Paciente
Suécia
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE


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[PMID]:27107715
[Au] Autor:Schilders KA; Eenjes E; van Riet S; Poot AA; Stamatialis D; Truckenmüller R; Hiemstra PS; Rottier RJ
[Ad] Endereço:Department of Pediatric Surgery, Erasmus Medical Center-Sophia Children's Hospital, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
[Ti] Título:Regeneration of the lung: Lung stem cells and the development of lung mimicking devices.
[So] Source:Respir Res;17:44, 2016 Apr 23.
[Is] ISSN:1465-993X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inspired by the increasing burden of lung associated diseases in society and an growing demand to accommodate patients, great efforts by the scientific community produce an increasing stream of data that are focused on delineating the basic principles of lung development and growth, as well as understanding the biomechanical properties to build artificial lung devices. In addition, the continuing efforts to better define the disease origin, progression and pathology by basic scientists and clinicians contributes to insights in the basic principles of lung biology. However, the use of different model systems, experimental approaches and readout systems may generate somewhat conflicting or contradictory results. In an effort to summarize the latest developments in the lung epithelial stem cell biology, we provide an overview of the current status of the field. We first describe the different stem cells, or progenitor cells, residing in the homeostatic lung. Next, we focus on the plasticity of the different cell types upon several injury-induced activation or repair models, and highlight the regenerative capacity of lung cells. Lastly, we summarize the generation of lung mimics, such as air-liquid interface cultures, organoids and lung on a chip, that are required to test emerging hypotheses. Moreover, the increasing collaboration between distinct specializations will contribute to the eventual development of an artificial lung device capable of assisting reduced lung function and capacity in human patients.
[Mh] Termos MeSH primário: Órgãos Bioartificiais
Transplante de Pulmão/instrumentação
Pulmão/citologia
Pulmão/crescimento & desenvolvimento
Regeneração/fisiologia
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Biomimética/instrumentação
Seres Humanos
Respiração Artificial/instrumentação
Transplante de Células-Tronco/métodos
Engenharia Tecidual/instrumentação
Engenharia Tecidual/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160425
[St] Status:MEDLINE
[do] DOI:10.1186/s12931-016-0358-z



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