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[PMID]:28631900
[Au] Autor:Glybochko PV; Alekseenko SN; Gubareva EA; Kuevda EV; Basov AA; Sotnichenko AS; Dzhimak SS; Gumenyuk IS; Egiev IK; Chechelyan VN; Nakokhov RZ; Lyasota OM; Teterin YV
[Ad] Endereço:I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
[Ti] Título:[Experimental development and rationale for a renal decellularization protocol with subsequent comprehensive assessment of the biological scaffold].
[So] Source:Urologiia;(2):5-13, 2017 Jun.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Chronic renal failure (CRF) is one of the most challenging problems of contemporary medicine. Patients with chronic renal failure usually need renal replacement therapy as either hemodialysis, peritoneal dialysis or a kidney transplant. The latter is the most promising option for end-stage kidney disease. However, the shortage of donor organs, the complexity of their delivery, the difficulty in finding an immunologically compatible donor and the need for lifelong immunosuppression triggered advances in modern tissue engineering. In this field, the primary priority is focused on developing bioengineered scaffolds with subsequent recellularization with autologous cells. Using such constructs would allow for solving both ethical and immunological problems of transplantation. The aim of this pilot study was to develop a new method of renal decellularization using small laboratory animals. MATERIALS AND METHODS: The study investigated the morphological structure of the obtained decellularized matrix and quantitatively tested DNA residues in the resulting scaffold. We proposed a new biophysical method for assessing the matrix quality using the EPR spectroscopy and conducted experiments on the matrix recellularization with mesenchymal multipotent stem cells to estimate cytotoxicity, cell viability and metabolic activity. RESULTS: The obtained decellularized renal matrix retained the native tissue architecture after a complete removal of the cell material, had no cytotoxic properties and supported cell adhesion and proliferation. CONCLUSION: All the above suggests that the proposed decellularization protocol is a promising method to produce tissue-engineered kidney constructs with possible clinical application in the foreseeable future.
[Mh] Termos MeSH primário: Falência Renal Crônica/terapia
Rim/anatomia & histologia
Rins Artificiais
Engenharia Tecidual
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Separação Celular
Rim/citologia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


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[PMID]:28279413
[Au] Autor:Sheth RA; Sheth AU
[Ad] Endereço:Department of Interventional Radiology, MD Anderson Cancer Center, Houston, TX. Electronic address: rasheth@mdanderson.org.
[Ti] Título:A Primer on Hemodialysis From an Interventional Radiology Perspective.
[So] Source:Tech Vasc Interv Radiol;20(1):9-13, 2017 Mar.
[Is] ISSN:1557-9808
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interventional radiologists play a central role in the care of patients with end-stage renal disease receiving renal replacement therapy. Ensuring that a patient׳s dialysis access remains suitable for high-quality dialysis is of paramount importance. However, although much has been spoken and written about endovascular techniques and outcomes based on angiographic criteria, little is generally known regarding the function and therefore the requirements of hemodialysis. In this article, we provide a heuristic overview of the mechanics of hemodialysis, with an emphasis on the "breaking points" in the extracorporeal circuit that trigger a patient׳s referral to Interventional Radiology. We also describe how dialysis quality is increasingly becoming linked with dialysis reimbursements. It is thus becoming progressively incumbent on the interventional radiologist to not only ensure that a patient receives high-quality outpatient dialysis but also that the patient׳s dialysis center meets its performance metrics.
[Mh] Termos MeSH primário: Derivação Arteriovenosa Cirúrgica
Cateterismo Venoso Central
Falência Renal Crônica/terapia
Diálise Renal
[Mh] Termos MeSH secundário: Derivação Arteriovenosa Cirúrgica/efeitos adversos
Derivação Arteriovenosa Cirúrgica/economia
Derivação Arteriovenosa Cirúrgica/normas
Cateterismo Venoso Central/efeitos adversos
Cateterismo Venoso Central/economia
Cateterismo Venoso Central/normas
Desenho de Equipamento
Planos de Pagamento por Serviço Prestado
Custos de Cuidados de Saúde
Seres Humanos
Falência Renal Crônica/diagnóstico
Rins Artificiais
Indicadores de Qualidade em Assistência à Saúde
Radiografia Intervencionista/efeitos adversos
Radiografia Intervencionista/economia
Radiografia Intervencionista/normas
Diálise Renal/economia
Diálise Renal/instrumentação
Diálise Renal/normas
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE


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[PMID]:28062681
[Au] Autor:Malhotra A; Garg P; Bishnoi AK; Sharma P; Wadhawa V; Shah K; Patel S; Ahirwar UK; Rodricks D; Pandya H
[Ad] Endereço:Department of Cardiovascular and Thoracic Surgery, U.N. Mehta Institute of Cardiology and Research Center (Affiliated to BJ Medical College, Ahmedabad), Ahmedabad, Gujarat, India.
[Ti] Título:Dialyzer-based cell salvage system: a superior alternative to conventional cell salvage in off-pump coronary artery bypass grafting.
[So] Source:Interact Cardiovasc Thorac Surg;24(4):489-497, 2017 04 01.
[Is] ISSN:1569-9285
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Our goal was to test the hypothesis that the use of a dialyzer-based cell salvage system during off-pump coronary artery bypass grafting (OPCABG) reduces requirements for homologous blood transfusions (HBT) and improves postoperative haemtochemical parameters. Methods: Data were prospectively collected for 222 patients who had OPCABG using 3 different cell salvage techniques: (1) dialyzer-based cell salvage (DBCS) ( n = 75), (2) conventional cell salvage (CCS) ( n = 73) and (3) without cell salvage (WCS) ( n = 74). Salvaged blood was transfused at the end of the operation. The primary outcome of the study was the amount of homologous blood transfused. Secondary outcomes were changes in haemtochemical parameters, postoperative bleeding, need for non-invasive ventilation (NIV), postoperative complications, renal dysfunction, clotting derangement, duration of intensive care unit (ICU) and hospital stay and mortality rates. Results: There were no deaths. In patients with >1000 ml blood loss, there was a significant reduction in HBT in the DBCS group (300 ± 161 ml) compared with the WCS group (550 ± 85 ml) ( P < 0.0001). Postoperative changes in haemtochemical parameters were significantly fewer in the DBCS group compared with the other 2 groups. The incidence of NIV ( P = 0.002), renal dysfunction ( P = 0.009) and postoperative complications ( P = 0.003) was least in the DBCS group and highest in the WCS group. Mean ICU stays were comparable ( P = 0.208); however, the mean hospital stay was significantly shorter in the DBCS group (6.08 ± 3.12 days) compared with the WCS group (7.54 ± 4.46 days) ( P = 0.022). There was no significant increase in coagulopathy in any group as suggested by comparable chest tube drainage ( P = 0.285) and comparable prothrombin time. Conclusions: The use of the DBCS system in OPCABG resulted in a significant reduction in HBT, improvement in postoperative levels of haemoglobin, platelets and albumin and reduction in complications without increased risk of coagulopathy.
[Mh] Termos MeSH primário: Ponte de Artéria Coronária sem Circulação Extracorpórea/efeitos adversos
Doença da Artéria Coronariana/terapia
Rins Artificiais
Hemorragia Pós-Operatória/etiologia
[Mh] Termos MeSH secundário: Idoso
Transfusão de Sangue
Feminino
Hemoglobinas
Seres Humanos
Tempo de Internação
Masculino
Meia-Idade
Hemorragia Pós-Operatória/terapia
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Hemoglobins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1093/icvts/ivw371


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[PMID]:27940061
[Au] Autor:Edens C; Wong J; Lyman M; Rizzo K; Nguyen D; Blain M; Horwich-Scholefield S; Moulton-Meissner H; Epson E; Rosenberg J; Patel PR
[Ad] Endereço:Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, GA; Epidemic Intelligence Service, Centers for Disease Control and Prevention, Atlanta, GA. Electronic address: wedens@cdc.gov.
[Ti] Título:Hemodialyzer Reuse and Gram-Negative Bloodstream Infections.
[So] Source:Am J Kidney Dis;69(6):726-733, 2017 Jun.
[Is] ISSN:1523-6838
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clusters of bloodstream infections caused by Burkholderia cepacia and Stenotrophomonas maltophilia are uncommon, but have been previously identified in hemodialysis centers that reprocessed dialyzers for reuse on patients. We investigated an outbreak of bloodstream infections caused by B cepacia and S maltophilia among hemodialysis patients in clinics of a dialysis organization. STUDY DESIGN: Outbreak investigation, including matched case-control study. SETTING & PARTICIPANTS: Hemodialysis patients treated in multiple outpatient clinics owned by a dialysis organization. PREDICTORS: Main predictors were dialyzer reuse, dialyzer model, and dialyzer reprocessing practice. OUTCOMES: Case patients had a bloodstream infection caused by B cepacia or S maltophilia; controls were patients without infection dialyzed at the same clinic on the same day as a case; results of environmental cultures and organism typing. RESULTS: 17 cases (9 B cepacia and 8 S maltophilia bloodstream infections) occurred in 5 clinics owned by the same dialysis organization. Case patients were more likely to have received hemodialysis with a dialyzer that had been used more than 6 times (matched OR, 7.03; 95% CI, 1.38-69.76) and to have been dialyzed with a specific reusable dialyzer (Model R) with sealed ends (OR, 22.87; 95% CI, 4.49-∞). No major lapses during dialyzer reprocessing were identified that could explain the outbreak. B cepacia was isolated from samples collected from a dialyzer header-cleaning machine from a clinic with cases and was indistinguishable from a patient isolate collected from the same clinic, by pulsed-field gel electrophoresis. Gram-negative bacteria were isolated from 2 reused Model R dialyzers that had undergone the facility's reprocessing procedure. LIMITATIONS: Limited statistical power and overmatching; few patient isolates and dialyzers available for testing. CONCLUSIONS: This outbreak was likely caused by contamination during reprocessing of reused dialyzers. Results of this and previous investigations demonstrate that exposing patients to reused dialyzers increases the risk for bloodstream infections. To reduce infection risk, providers should consider implementing single dialyzer use whenever possible.
[Mh] Termos MeSH primário: Bacteriemia/epidemiologia
Infecções por Burkholderia/epidemiologia
Surtos de Doenças
Desinfecção/estatística & dados numéricos
Infecções por Bactérias Gram-Negativas/epidemiologia
Falência Renal Crônica/terapia
Rins Artificiais/estatística & dados numéricos
Stenotrophomonas maltophilia/imunologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Burkholderia cepacia
Estudos de Casos e Controles
Descontaminação
Contaminação de Equipamentos
Feminino
Seres Humanos
Controle de Infecções
Rins Artificiais/microbiologia
Masculino
Meia-Idade
Diálise Renal
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27709305
[Au] Autor:Saito M; Iwasaki K
[Ad] Endereço:Cooperative Major in Advanced Biomedical Sciences, Joint Graduate School of Tokyo Women's Medical University and Waseda University, Tokyo, Japan.
[Ti] Título:Analysis of the safety evaluation for premarketing clinical trials of hemodialyzer and of postmarketing safety reports of hemodialyzer in Japan and the US: insights into the construction of a sophisticated premarketing evaluation.
[So] Source:J Artif Organs;20(1):62-70, 2017 Mar.
[Is] ISSN:1619-0904
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Our aim was to conduct a scoping review of the regulations for hemodialyzers in the safety evaluation in Japan and the United States, and to evaluate the criteria for premarketing clinical trials and postmarketing safety reports to inform the development of a sophisticated premarketing evaluation in Japan. Regulations for approval of hemodialyzers were identified from the databases of the Ministry of Health, Labor and Welfare in Japan and the Federal Drug Agency (FDA) in the United States (US). The criteria for premarket clinical trials and postmarketing safety reports were evaluated for both countries. Standards in Japan required evaluation of blood compatibility and reporting of acute adverse effects by a premarketing clinical trial in 6 of 86 applications with semipermeable membrane materials deemed to be different to those of previously approved devices from 1983 to 31 August 2015. By comparison, the clinical trial was required in one of 545 approvals in the US from 1976 to 29 January 2016, but blood compatibility was not the point. All postmarketing adverse effects identified in Japan were included in the set of 'warnings'. The more stringent requirements for evaluation of blood compatibility and acute adverse effects in Japan seemed to be related to differences in the history of quality management systems for medical devices between the two countries. This study revealed that there were differences between Japan and the US in requiring the premarketing clinical trials for the hemodialyzers. Our findings could be useful for constructing sophisticated premarketing safety evaluation.
[Mh] Termos MeSH primário: Ensaios Clínicos como Assunto
Rins Artificiais/normas
Vigilância de Produtos Comercializados
Segurança
[Mh] Termos MeSH secundário: Seres Humanos
Japão
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1007/s10047-016-0928-3


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Registro de Ensaios Clínicos
[PMID]:27884120
[Au] Autor:Donati G; Moretti MI; Baraldi O; Spazzoli A; Capelli I; Comai G; Marchetti A; Sarma M; Mancini R; La Manna G
[Ad] Endereço:Nephrology Dialysis and Renal Transplantation Unit, S.Orsola University Hospital, Via Massarenti 9, 40138, Bologna, Italy.
[Ti] Título:Removal of free light chains in hemodialysis patients without multiple myeloma: a crossover comparison of three different dialyzers.
[So] Source:BMC Nephrol;17(1):193, 2016 11 25.
[Is] ISSN:1471-2369
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Immunoglobulin light chains are classified as middle molecule uremic toxins able to interact with B lymphocyte membranes leading to the activation of transmembrane signaling. The ensuing impairment of neutrophil function can contribute to the chronic inflammation state of uremic patients, and the increased risk of bacterial infections or vascular calcifications. The aim of this crossover observational study was to assess the difference in free light chain removal by three different hemodialysis filters in patients not affected by multiple myeloma. METHODS: Free light chain removal was compared in the polymethylmethacrylate (PMMA) membrane Filtryzer BK-F, the polyphenylene HFR17 filter and the conventional polysulfone filter F7HPS. Twenty chronic hemodialysis patients were enrolled: mean age was 67.7 ± 17.0 years, M/F = 14/6, dialysis vintage (months) 25.5 ± 32.0. The patients were randomized into two groups of treatment lasting 6 weeks each. The dialysis sessions checked were the midweek sessions and the blood was drawn at times 0, 120' and 240'. Kappa (k) and lambda (λ) light chain levels, ß2microglobulin (ß2M), C reactive protein (CRP) and albumin were checked. RESULTS: K light chain levels were 345.0 ± 100.0 mg/L, λ light chains were 121.4 ± 27.0 mg/L. The values of k light chains at times 120' and 240' were significantly lower with PMMA and HFR17 than those obtained with F7. The reduction ratio per session (RRs) for k light chains was 44.1 ± 4.3% with HFR17, 55.3 ± 3.4% with PMMA, 25.7 ± 8.3% with F7 (p = 0.018). The RRs for λ light chains was 30.3 ± 2.9% with HFR17, 37.8 ± 17.3% with PMMA, 14.0 ± 3.9% with F7 (p = 0.032). As to ß2M, RRs was 42.4 ± 3.2% with HFR17 vs. 33.9 ± 2.8% with PMMA vs. 6.3 ± 1.9% with F7 (p = 0.022). The three filters tested showed no differences in CRP or albumin levels. CONCLUSION: In terms of light chain and ß2M removal, the PMMA and on-line HFR filters are similar and both are significantly more effective than the F7 filter in chronic dialysis patients. TRIAL REGISTRATION: The present trial was registered retrospectively ( NCT02950389 , 31/10/2016).
[Mh] Termos MeSH primário: Cadeias Leves de Imunoglobulina/sangue
Rins Artificiais
Polímeros
Polimetil Metacrilato
Diálise Renal/métodos
Sulfonas
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Estudos Cross-Over
Feminino
Seres Humanos
Rins Artificiais/normas
Masculino
Meia-Idade
Mieloma Múltiplo
Polímeros/normas
Polimetil Metacrilato/normas
Diálise Renal/normas
Sulfonas/normas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; OBSERVATIONAL STUDY; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin Light Chains); 0 (Polymers); 0 (Sulfones); 25135-51-7 (polysulfone P 1700); 9011-14-7 (Polymethyl Methacrylate); 9016-75-5 (polyphenylene sulfide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE


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[PMID]:27632042
[Au] Autor:Loughlin S; Concepcion D; Gonzalez G; Maltais JA; Neuland C; Pulliam J; Treu D; Williams RJ
[Ti] Título:A Roundtable Discussion: Exploring Major Trends in Dialysis Practice and Technology.
[So] Source:Biomed Instrum Technol;50(5):364-9, 2016 Sep-Oct.
[Is] ISSN:0899-8205
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Biorretroalimentação Psicológica/instrumentação
Diálise/instrumentação
Testes de Função Renal/instrumentação
Rins Artificiais/tendências
Insuficiência Renal/terapia
Terapia Assistida por Computador/instrumentação
[Mh] Termos MeSH secundário: Diálise/tendências
Desenho de Equipamento
Análise de Falha de Equipamento
Seres Humanos
Testes de Função Renal/tendências
Terapia Assistida por Computador/tendências
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE
[do] DOI:10.2345/0899-8205-50.5.364


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[PMID]:27501003
[Au] Autor:Kakuta T; Komaba H; Takagi N; Takahashi Y; Suzuki H; Hyodo T; Nagaoka M; Tanaka R; Iwao S; Ishida M; Kobayashi H; Saito A; Fukagawa M
[Ad] Endereço:Division of Nephrology, Endocrinology and Metabolism, Department of Medicine, Tokai University Hachioji Hospital, Hachioji, Tokyo, Japan.
[Ti] Título:A Prospective Multicenter Randomized Controlled Study on Interleukin-6 Removal and Induction by a new Hemodialyzer With Improved Biocompatibility in Hemodialysis Patients: A Pilot Study.
[So] Source:Ther Apher Dial;20(6):569-578, 2016 Dec.
[Is] ISSN:1744-9987
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:We compared interleukin-6 (IL-6) removal and induction between conventional polysulfone (Con) and TORAYLIGHT NV (NV) dialyzers in hemodialysis patients. Twenty patients on Con with high IL-6 concentrations (2.7-8.5 pg/mL) were randomized to Con or NV group. Dialyzer performance was determined in NV group while patients were on Con and after being switched onto NV. Erythropoiesis-stimulating agent (ESA) response index (ERI) was assessed every 4 months for one year. IL-6 clearance was comparable between Con and NV. IL-6 removal rates were comparable for the first 1 h, but were higher with NV for the entire session (P = 0.03). Before-to-during-dialysis IL-6 concentration ratios were lower with NV on the venous side after the session (P = 0.03). During the one-year study, hemoglobin was lower in Con group than in NV group at month 8 (P = 0.046). ERI decreased in NV and increased in Con group, with a significant difference between the groups (P = 0.002). NV and Con are comparable in removing IL-6 and both induce IL-6. However, the data suggest that NV induces less IL-6, which may reduce the risk of ESA hyporesponsiveness.
[Mh] Termos MeSH primário: Interleucina-6
Falência Renal Crônica/terapia
Rins Artificiais
Diálise Renal/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Projetos Piloto
Polímeros
Estudos Prospectivos
Sulfonas
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Interleukin-6); 0 (Polymers); 0 (Sulfones); 25135-51-7 (polysulfone P 1700)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.1111/1744-9987.12454


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[PMID]:27474967
[Au] Autor:Cho S; Islas-Robles A; Nicolini AM; Monks TJ; Yoon JY
[Ad] Endereço:Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ, 85721-0038 USA.
[Ti] Título:In situ, dual-mode monitoring of organ-on-a-chip with smartphone-based fluorescence microscope.
[So] Source:Biosens Bioelectron;86:697-705, 2016 Dec 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of organ-on-a-chip (OOC) platforms enables improved simulation of the human kidney's response to nephrotoxic drugs. The standard method of analyzing nephrotoxicity from existing OOC has majorly consisted of invasively collecting samples (cells, lysates, media, etc.) from an OOC. Such disruptive analyses potentiate contamination, disrupt the replicated in vivo environment, and require expertize to execute. Moreover, traditional analyses, including immunofluorescence microscopy, immunoblot, and microplate immunoassay are essentially not in situ and require substantial time, resources, and costs. In the present work, the incorporation of fluorescence nanoparticle immunocapture/immunoagglutination assay into an OOC enabled dual-mode monitoring of drug-induced nephrotoxicity in situ. A smartphone-based fluorescence microscope was fabricated as a handheld in situ monitoring device attached to an OOC. Both the presence of γ-glutamyl transpeptidase (GGT) on the apical brush-border membrane of 786-O proximal tubule cells within the OOC surface, and the release of GGT to the outflow of the OOC were evaluated with the fluorescence scatter detection of captured and immunoagglutinated anti-GGT conjugated nanoparticles. This dual-mode assay method provides a novel groundbreaking tool to enable the internal and external in situ monitoring of the OOC, which may be integrated into any existing OOCs to facilitate their subsequent analyses.
[Mh] Termos MeSH primário: Bioprótese
Imunoensaio/instrumentação
Rim/imunologia
Dispositivos Lab-On-A-Chip
Microscopia de Fluorescência/instrumentação
Smartphone
[Mh] Termos MeSH secundário: Testes de Aglutinação/instrumentação
Linhagem Celular
Desenho de Equipamento
Análise de Falha de Equipamento
Seres Humanos
Imunoensaio/métodos
Rim/efeitos dos fármacos
Rins Artificiais
Microscopia de Fluorescência/métodos
Aplicativos Móveis
Técnicas de Cultura de Órgãos/instrumentação
Técnicas de Cultura de Órgãos/métodos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Testes de Toxicidade/instrumentação
Testes de Toxicidade/métodos
Interface Usuário-Computador
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE


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[PMID]:27395800
[Au] Autor:Chevtchik NV; Fedecostante M; Jansen J; Mihajlovic M; Wilmer M; Rüth M; Masereeuw R; Stamatialis D
[Ad] Endereço:Department of Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
[Ti] Título:Upscaling of a living membrane for bioartificial kidney device.
[So] Source:Eur J Pharmacol;790:28-35, 2016 Nov 05.
[Is] ISSN:1879-0712
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The limited removal of metabolic waste products in dialyzed kidney patients leads to high morbidity and mortality. One powerful solution for a more complete removal of those metabolites might be offered by a bioartificial kidney device (BAK), which contains a hybrid "living membrane" with functional proximal tubule epithelial cells (PTEC). These cells are supported by an artificial functionalized hollow fiber membrane (HFM) and are able to actively remove the waste products. In our earlier studies, conditionally immortalized human PTEC (ciPTEC) showed to express functional organic cationic transporter 2 (OCT2) when seeded on small size flat or hollow fiber polyethersulfone (PES) membranes. Here, an upscaled "living membrane" is presented. We developed and assessed the functionality of modules containing three commercially available MicroPES HFM supporting ciPTEC. The HFM were optimally coated with L-Dopa and collagen IV to support a uniform and tight monolayer formation of matured ciPTEC under static culturing conditions. Both abundant expression of zonula occludens-1 (ZO-1) protein and limited diffusion of FITC-inulin confirm a clear barrier function of the monolayer. Furthermore, the uptake of 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP ), a fluorescent OCT2 substrate, was studied in absence and presence of known OCT inhibitors, such as cimetidine and a cationic uremic solutes mixture. The ASP uptake by the living upscaled membrane was decreased by 60% in the presence of either inhibitor, proving the active function of OCT2. In conclusion, this study presents a successful upscaling of a living membrane with active organic cation transport as a support for BAK device.
[Mh] Termos MeSH primário: Rins Artificiais
Membranas Artificiais
[Mh] Termos MeSH secundário: Transporte Biológico
Células Epiteliais/citologia
Células Epiteliais/metabolismo
Fluoresceína-5-Isotiocianato/análogos & derivados
Fluoresceína-5-Isotiocianato/metabolismo
Inulina/análogos & derivados
Inulina/metabolismo
Túbulos Renais Proximais/citologia
Proteínas de Transporte de Cátions Orgânicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (FITC-inulin); 0 (Membranes, Artificial); 0 (Organic Cation Transport Proteins); 9005-80-5 (Inulin); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE



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