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  1 / 2887 MEDLINE  
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[PMID]:28745619
[Au] Autor:Barradale F; Sinha K; Lebestky T
[Ad] Endereço:Department of Biology, Williams College.
[Ti] Título:Quantification of Drosophila Grooming Behavior.
[So] Source:J Vis Exp;(125), 2017 Jul 19.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drosophila grooming behavior is a complex multi-step locomotor program that requires coordinated movement of both forelegs and hindlegs. Here we present a grooming assay protocol and novel chamber design that is cost-efficient and scalable for either small or large-scale studies of Drosophila grooming. Flies are dusted all over their body with Brilliant Yellow dye and given time to remove the dye from their bodies within the chamber. Flies are then deposited in a set volume of ethanol to solubilize the dye. The relative spectral absorbance of dye-ethanol samples for groomed versus ungroomed animals are measured and recorded. The protocol yields quantitative data of dye accumulation for individual flies, which can be easily averaged and compared across samples. This allows experimental designs to easily evaluate grooming ability for mutant animal studies or circuit manipulations. This efficient procedure is both versatile and scalable. We show work-flow of the protocol and comparative data between WT animals and mutant animals for the Drosophila type I Dopamine Receptor (DopR).
[Mh] Termos MeSH primário: Comportamento Animal/fisiologia
Drosophila/metabolismo
Asseio Animal/fisiologia
[Mh] Termos MeSH secundário: Animais
Drosophila/citologia
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55231


  2 / 2887 MEDLINE  
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[PMID]:28743598
[Au] Autor:Ebihara K; Fujiwara H; Awale S; Dibwe DF; Araki R; Yabe T; Matsumoto K
[Ad] Endereço:Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
[Ti] Título:Decrease in endogenous brain allopregnanolone induces autism spectrum disorder (ASD)-like behavior in mice: A novel animal model of ASD.
[So] Source:Behav Brain Res;334:6-15, 2017 09 15.
[Is] ISSN:1872-7549
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Autism spectrum disorder (ASD) is a neurodevelopmental disorder with core symptoms of social impairments and restrictive repetitive behaviors. Recent evidence has implicated a dysfunction in the GABAergic system in the pathophysiology of ASD. We investigated the role of endogenous allopregnanolone (ALLO), a neurosteroidal positive allosteric modulator of GABA receptors, in the regulation of ASD-like behavior in male mice using SKF105111 (SKF), an inhibitor of type I and type II 5α-reductase, a rate-limiting enzyme of ALLO biosynthesis. SKF impaired sociability-related performance, as analyzed by three different tests; i.e., the 3-chamber test and social interaction in the open field and resident-intruder tests, without affecting olfactory function elucidated by the buried food test. SKF also induced repetitive grooming behavior without affecting anxiety-like behavior. SKF had no effect on short-term spatial working memory or long-term fear memory, but enhanced latent learning ability in male mice. SKF-induced ASD-like behavior in male mice was abolished by the systemic administration of ALLO (1mg/kg, i.p.) and methylphenidate (MPH: 2.5mg/kg, i.p.), a dopamine transporter inhibitor. The effects of SKF on brain ALLO contents in male mice were reversed by ALLO, but not MPH. On the other hand, SKF failed to induce ASD-like behavior or a decline in brain ALLO contents in female mice. These results suggest that ALLO regulates episodes of ASD-like behavior by positively modulating the function of GABA receptors linked to the dopaminergic system. Moreover, a sex-dependently induced decrease in brain ALLO contents may provide an animal model to study the main features of ASD.
[Mh] Termos MeSH primário: Androstanos
Transtorno do Espectro Autista/metabolismo
Encéfalo/metabolismo
Modelos Animais de Doenças
Pregnanolona/deficiência
[Mh] Termos MeSH secundário: Inibidores de 5-alfa Redutase/farmacologia
Animais
Ansiedade/metabolismo
Transtorno do Espectro Autista/tratamento farmacológico
Transtorno do Espectro Autista/psicologia
Encéfalo/efeitos dos fármacos
Medo/fisiologia
Feminino
Asseio Animal/fisiologia
Aprendizagem/fisiologia
Masculino
Memória/fisiologia
Metilfenidato/farmacologia
Camundongos Endogâmicos ICR
Psicotrópicos/farmacologia
Caracteres Sexuais
Comportamento Social
Comportamento Estereotipado/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5-alpha Reductase Inhibitors); 0 (Androstanes); 0 (Psychotropic Drugs); 0 (SKF 105111); 207ZZ9QZ49 (Methylphenidate); BXO86P3XXW (Pregnanolone)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  3 / 2887 MEDLINE  
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[PMID]:28902896
[Au] Autor:Maraz A; Hende B; Urbán R; Demetrovics Z
[Ad] Endereço:Institute of Psychology, Eötvös Loránd University, Budapest, Hungary.
[Ti] Título:Pathological grooming: Evidence for a single factor behind trichotillomania, skin picking and nail biting.
[So] Source:PLoS One;12(9):e0183806, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although trichotillomania (TTM), skin picking (SP), and nail biting (NB) have been receiving growing scientific attention, the question as to whether these disorders can be regarded as separate entities or they are different manifestations of the same underlying tendency is unclear. Data were collected online in a community survey, yielding a sample of 2705 participants (66% women, mean age: 29.1, SD: 8.6). Hierarchical factor analysis was used to identify a common latent factor and the multiple indicators and multiple causes (MIMIC) modelling was applied to test the predictive effect of borderline personality disorder symptoms, impulsivity, distress and self-esteem on pathological grooming. Pearson correlation coefficients between TTM, SP and NB were between 0.13 and 0.29 (p < 0.01). The model yielded an excellent fit to the data (CFI = 0.992, TLI = 0.991, χ2 = 696.65, p < 0.001, df = 222, RMSEA = 0.030, Cfit of RMSEA = 1.000), supporting the existence of a latent factor. The MIMIC model indicated an adequate fit (CFI = 0.993, TLI = 0.992, χ2 = 655.8, p < 0.001, df = 307, RMSEA = 0.25, CI: 0.022-0.028, pclose = 1.000). TTM, SP and NB each were loaded significantly on the latent factor, indicating the presence of a general grooming factor. Impulsivity, psychiatric distress and contingent self-esteem had significant predictive effects, whereas borderline personality disorder had a nonsignificant predictive effect on the latent factor. We found evidence that the category of pathological grooming is meaningful and encompasses three symptom manifestations: trichotillomania, skin picking and nail biting. This latent underlying factor is not better explained by indicators of psychopathology, which supports the notion that the urge to self-groom, rather than general psychiatric distress, impulsivity, self-esteem or borderline symptomatology, is what drives individual grooming behaviours.
[Mh] Termos MeSH primário: Asseio Animal
Hábito de Roer Unhas/psicologia
Transtorno Obsessivo-Compulsivo/etiologia
Comportamento Autodestrutivo/etiologia
Pele/lesões
Estresse Psicológico/complicações
Tricotilomania/etiologia
[Mh] Termos MeSH secundário: Adulto
Animais
Transtornos Disruptivos, de Controle do Impulso e da Conduta/classificação
Transtornos Disruptivos, de Controle do Impulso e da Conduta/diagnóstico
Transtornos Disruptivos, de Controle do Impulso e da Conduta/epidemiologia
Transtornos Disruptivos, de Controle do Impulso e da Conduta/etiologia
Feminino
Seres Humanos
Masculino
Transtorno Obsessivo-Compulsivo/classificação
Transtorno Obsessivo-Compulsivo/diagnóstico
Transtorno Obsessivo-Compulsivo/epidemiologia
Comportamento Autodestrutivo/epidemiologia
Pele/patologia
Estresse Psicológico/epidemiologia
Tricotilomania/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183806


  4 / 2887 MEDLINE  
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[PMID]:28877178
[Au] Autor:Koch L; Lunau K; Wester P
[Ad] Endereço:Institute of Sensory Ecology, Heinrich-Heine-University, Düsseldorf, Germany.
[Ti] Título:To be on the safe site - Ungroomed spots on the bee's body and their importance for pollination.
[So] Source:PLoS One;12(9):e0182522, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flower-visiting bees collect large quantities of pollen to feed their offspring. Pollen deposited in the bees' transport organs is lost for the flowers' pollination. It has been hypothesised that specific body areas, bees cannot groom, serve as 'safe sites' for pollen transfer between flowers. For the first time, we experimentally demonstrated the position, area and pollen amount of safe sites at the examples of Apis mellifera and Bombus terrestris by combining artificial contamination of the bees' body with pine or sunflower pollen and the subsequent bees' incomplete grooming. We found safe sites on the forehead, the dorsal thorax and waist, and on the dorsal and ventral abdomen of the bees. These areas were less groomed by the bees' legs. The largest amount of pollen was found on the waist, followed by the dorsal areas of thorax and abdomen. At the example of Salvia pratensis, S. officinalis and Borago officinalis, we experimentally demonstrated with fluorescent dye that the flowers' pollen-sacs and stigma contact identical safe sites. These results confirm that pollen deposition on the bees' safe sites improves pollen transfer to stigmas of conspecific flowers sti. Future research will demonstrate the importance of safe sites for plant pollination under field conditions.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Pigmentação/fisiologia
Polinização/fisiologia
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
Flores/fisiologia
Asseio Animal
Helianthus/fisiologia
Pinus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182522


  5 / 2887 MEDLINE  
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[PMID]:28683272
[Au] Autor:Neukomm LJ; Burdett TC; Seeds AM; Hampel S; Coutinho-Budd JC; Farley JE; Wong J; Karadeniz YB; Osterloh JM; Sheehan AE; Freeman MR
[Ad] Endereço:Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA, USA. Electronic address: lukas.neukomm@gmail.com.
[Ti] Título:Axon Death Pathways Converge on Axundead to Promote Functional and Structural Axon Disassembly.
[So] Source:Neuron;95(1):78-91.e5, 2017 Jul 05.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Axon degeneration is a hallmark of neurodegenerative disease and neural injury. Axotomy activates an intrinsic pro-degenerative axon death signaling cascade involving loss of the NAD biosynthetic enzyme Nmnat/Nmnat2 in axons, activation of dSarm/Sarm1, and subsequent Sarm-dependent depletion of NAD . Here we identify Axundead (Axed) as a mediator of axon death. axed mutants suppress axon death in several types of axons for the lifespan of the fly and block the pro-degenerative effects of activated dSarm in vivo. Neurodegeneration induced by loss of the sole fly Nmnat ortholog is also fully blocked by axed, but not dsarm, mutants. Thus, pro-degenerative pathways activated by dSarm signaling or Nmnat elimination ultimately converge on Axed. Remarkably, severed axons morphologically preserved by axon death pathway mutations remain integrated in circuits and able to elicit complex behaviors after stimulation, indicating that blockade of axon death signaling results in long-term functional preservation of axons.
[Mh] Termos MeSH primário: Proteínas do Domínio Armadillo/genética
Axônios/metabolismo
Proteínas do Citoesqueleto/genética
Proteínas de Drosophila/genética
Nicotinamida-Nucleotídeo Adenililtransferase/genética
Degeneração Walleriana/genética
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Proteínas do Domínio Armadillo/metabolismo
Antenas de Artrópodes/lesões
Antenas de Artrópodes/inervação
Axotomia
Comportamento Animal
Western Blotting
Linhagem Celular
Proteínas do Citoesqueleto/metabolismo
Proteínas de Drosophila/metabolismo
Drosophila melanogaster
Asseio Animal
Imunidade Ativa
NAD/metabolismo
Neurônios/metabolismo
Nicotinamida-Nucleotídeo Adenililtransferase/metabolismo
Optogenética
Degeneração Walleriana/metabolismo
Asas de Animais/lesões
Asas de Animais/inervação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Armadillo Domain Proteins); 0 (Cytoskeletal Proteins); 0 (Drosophila Proteins); 0 (Sarm protein, Drosophila); 0 (axundead protein, Drosophila); 0U46U6E8UK (NAD); EC 2.7.7.1 (Nicotinamide-Nucleotide Adenylyltransferase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE


  6 / 2887 MEDLINE  
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[PMID]:28622341
[Au] Autor:Nganso BT; Fombong AT; Yusuf AA; Pirk CWW; Stuhl C; Torto B
[Ad] Endereço:International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.
[Ti] Título:Hygienic and grooming behaviors in African and European honeybees-New damage categories in Varroa destructor.
[So] Source:PLoS One;12(6):e0179329, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Varroa destructor is an ectoparasitic pest of honeybees, and a threat to the survival of the apiculture industry. Several studies have shown that unlike European honeybees, African honeybee populations appear to be minimally affected when attacked by this mite. However, little is known about the underlying drivers contributing to survival of African honeybee populations against the mite. We hypothesized that resistant behavioral defenses are responsible for the survival of African honeybees against the ectoparasite. We tested this hypothesis by comparing grooming and hygienic behaviors in the African savannah honeybee Apis mellifera scutellata in Kenya and A. mellifera hybrids of European origin in Florida, USA against the mite. Grooming behavior was assessed by determining adult mite infestation levels, daily mite fall per colony and percentage mite damage (as an indicator of adult grooming rate), while hygienic behavior was assessed by determining the brood removal rate after freeze killing a section of the brood. Our results identified two additional undescribed damaged mite categories along with the six previously known damage categories associated with the grooming behavior of both honeybee subspecies. Adult mite infestation level was approximately three-fold higher in A. mellifera hybrids of European origin than in A. m. scutellata, however, brood removal rate, adult grooming rate and daily natural mite fall were similar in both honeybee subspecies. Unlike A. mellifera hybrids of European origin, adult grooming rate and brood removal rate did not correlate with mite infestation levels on adult worker honeybee of A. m. scutellata though they were more aggressive towards the mites than their European counterparts. Our results provide valuable insights into the tolerance mechanisms that contribute to the survival of A. m. scutellata against the mite.
[Mh] Termos MeSH primário: Abelhas/parasitologia
Asseio Animal
Varroidae
[Mh] Termos MeSH secundário: África
Animais
Europa (Continente)
Florida
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179329


  7 / 2887 MEDLINE  
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[PMID]:28542485
[Au] Autor:Sauce B; Goes CP; Forti I; O do Monte BG; Watanabe IM; Cunha J; Peripato AC
[Ad] Endereço:Department of Psychology, Program in Behavioral and Systems Neuroscience Rutgers University, Piscataway, United States of America.
[Ti] Título:A link between thrifty phenotype and maternal care across two generations of intercrossed mice.
[So] Source:PLoS One;12(5):e0177954, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maternal effects are causal influences from mother to offspring beyond genetic information, and have lifelong consequences for multiple traits. Previously, we reported that mice whose mothers did not nurse properly had low birth weight followed by rapid fat accumulation and disturbed development of some organs. That pattern resembles metabolic syndromes known collectively as the thrifty phenotype, which is believed to be an adaptation to a stressful environment which prepares offspring for reduced nutrient supply. The potential link between maternal care, stress reactivity, and the thrifty phenotype, however, has been poorly explored in the human and animal literature: only a couple of studies even mention (much less, test) these concepts under a cohesive framework. Here, we explored this link using mice of the parental inbred strains SM/J and LG/J-who differ dramatically in their maternal care-and the intercrossed generations F1 and F2. We measured individual differences in 15 phenotypes and used structural equation modeling to test our hypotheses. We found a remarkable relationship between thrifty phenotype and lower quality of maternal behaviors, including nest building, pup retrieval, grooming/licking, and nursing. To our knowledge, this is the first study to show, in any mammal, a clear connection between the natural variation in thrifty phenotype and maternal care. Both traits in the mother also had a substantial effect on survival rate in the F3 offspring. To our surprise, however, stress reactivity seemed to play no role in our models. Furthermore, the strain of maternal grandmother, but not of paternal grandmother, affected the variation of maternal care in F2 mice, and this effect was mediated by thrifty phenotype in F2. Since F1 animals were all genetically identical, this finding suggests that maternal effects pass down both maternal care and thrifty phenotype in these mice across generations via epigenetic transmission.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Comportamento Materno/psicologia
Fenômenos Fisiológicos da Nutrição/fisiologia
Efeitos Tardios da Exposição Pré-Natal
[Mh] Termos MeSH secundário: Animais
Metabolismo Energético
Feminino
Asseio Animal
Seres Humanos
Camundongos
Fenótipo
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177954


  8 / 2887 MEDLINE  
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[PMID]:28535866
[Au] Autor:Moore ES; Cleland TA; Williams WO; Peterson CM; Singh B; Southard TL; Pasch B; Labitt RN; Daugherity EK
[Ad] Endereço:Departments of Biomedical Sciences, Cornell Lab of Ornithology, Cornell University, Ithaca, New York;, Email: esm84@cornell.edu.
[Ti] Título:Comparing Phlebotomy by Tail Tip Amputation, Facial Vein Puncture, and Tail Vein Incision in C57BL/6 Mice by Using Physiologic and Behavioral Metrics of Pain and Distress.
[So] Source:J Am Assoc Lab Anim Sci;56(3):307-317, 2017 May 01.
[Is] ISSN:1559-6109
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tail tip amputation with minimal restraint is not widely used for mouse phlebotomy. In part, this infrequency may reflect policies influenced by tail tip amputation procedures for genotyping, which involve greater handling and tissue removal. To assess tail tip amputation with minimal restraint as a phlebotomy technique, we compared it with 2 more common methods: scruffing with facial vein puncture and lateral tail vein incision with minimal restraint. Blood glucose levels, audible and ultrasonic vocalizations, postphlebotomy activity and grooming behavior, open field and elevated plus maze behaviors, nest-building scores, and histologic changes at the phlebotomy site were evaluated. Mice in the facial vein phlebotomy group produced more audible vocalizations, exhibited lower postphlebotomy activity in the open field, and had more severe histologic changes than did mice in the tail incision and tail tip amputation groups. Facial vein phlebotomy did not affect grooming behavior relative to sham groups, whereas tail vein incision-but not tail tip amputation-increased tail grooming compared with that in control mice. Blood glucose levels, nest-building scores, and elevated plus maze behavior did not differ between groups, and no mice in any group produced ultrasonic vocalizations. Tail tip amputation mice did not perform differently than sham mice in any metric analyzed, indicating that this technique is a potentially superior method of blood collection in mice in terms of animal wellbeing.
[Mh] Termos MeSH primário: Camundongos
Flebotomia/veterinária
[Mh] Termos MeSH secundário: Animais
Comportamento Animal
Asseio Animal
Veias Jugulares
Camundongos Endogâmicos C57BL
Dor
Flebotomia/métodos
Punções
Distribuição Aleatória
Cauda
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE


  9 / 2887 MEDLINE  
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[PMID]:28426681
[Au] Autor:Vieira GLT; Lossie AC; Lay DC; Radcliffe JS; Garner JP
[Ad] Endereço:Department of Animal Sciences, Purdue University, West Lafayette, Indiana, United States of America.
[Ti] Título:Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania.
[So] Source:PLoS One;12(4):e0175222, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Barbering, where a "barber" mouse plucks hair from its cagemates or itself, is both a spontaneously occurring abnormal behavior in mice and a well validated model of Trichotillomania (TTM). N-Acetylcysteine, (NAC) a cysteine derived food additive, is remarkably effective in treating TTM patients, but its mechanism of action is unknown. Reactive Oxygen Species (ROS), also known as free radicals, form as a natural byproduct of the normal metabolism of oxygen. Under normal circumstances, cells are able to defend themselves against ROS damage with antioxidant pathways. NAC is the precursor to the main antioxidant produced to defend the brain. Therefore, we hypothesized that barbering is a disease of oxidative stress, whereby ROS and/or a failure of antioxidant defenses leads to neuronal damage that induces barbering in susceptible animals. We tested this hypothesis in 32 female C57BL/6J mice by treating half with 1g/kg BW/day of NAC in their diet, and testing for protection against developing barbering behavior and curing of barbering behavior, and simultaneously testing for a panel of biomarkers of oxidative stress. NAC reduced the chance that mice would be barbers, and this effect did not differ between healthy (i.e. prevention) and affected animals (i.e. cure). Barbering animals had elevated urinary antioxidant capacity, indicative of oxidative stress, at all timepoints. Additionally, after treatment the risk of barbering increased with decreasing hydroxy-2'-deoxyguanosine (8-OHdG) levels, and with increasing glutathione (GSH) and oxidized glutathione (GSSG) levels, further indicating that barbering mice were under oxidative stress regardless of treatment with NAC. We did not find compelling evidence that urinary total antioxidant capacity, or urinary 8-OHdG, could predict response to NAC treatment. We conclude that NAC is effective in preventing and/or curing barbering at least in part by promoting GSH synthesis, thereby preventing oxidative damage.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Modelos Animais de Doenças
Asseio Animal
Estresse Oxidativo
Tricotilomania/metabolismo
[Mh] Termos MeSH secundário: Acetilcisteína/uso terapêutico
Animais
Desoxiguanosina/análogos & derivados
Desoxiguanosina/metabolismo
Desoxiguanosina/urina
Camundongos
Camundongos Endogâmicos C57BL
Tricotilomania/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 88847-89-6 (8-oxo-7-hydrodeoxyguanosine); G9481N71RO (Deoxyguanosine); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175222


  10 / 2887 MEDLINE  
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[PMID]:28403242
[Au] Autor:Wu JL; Zhou CX; Wu PJ; Xu J; Guo YQ; Xue F; Getachew A; Xu SF
[Ad] Endereço:Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Brain metabolomic profiling of eastern honey bee (Apis cerana) infested with the mite Varroa destructor.
[So] Source:PLoS One;12(4):e0175573, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mite Varroa destructor is currently the greatest threat to apiculture as it is causing a global decrease in honey bee colonies. However, it rarely causes serious damage to its native hosts, the eastern honey bees Apis cerana. To better understand the mechanism of resistance of A. cerana against the V. destructor mite, we profiled the metabolic changes that occur in the honey bee brain during V. destructor infestation. Brain samples were collected from infested and control honey bees and then measured using an untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based global metabolomics method, in which 7918 and 7462 ions in ESI+ and ESI- mode, respectively, were successfully identified. Multivariate statistical analyses were applied, and 64 dysregulated metabolites, including fatty acids, amino acids, carboxylic acid, and phospholipids, amongst others, were identified. Pathway analysis further revealed that linoleic acid metabolism; propanoate metabolism; and glycine, serine, and threonine metabolism were acutely perturbed. The data obtained in this study offer insight into the defense mechanisms of A. cerana against V. destructor mites and provide a better method for understanding the synergistic effects of parasitism on honey bee colonies.
[Mh] Termos MeSH primário: Abelhas/metabolismo
Encéfalo/metabolismo
Varroidae/fisiologia
[Mh] Termos MeSH secundário: Animais
Abelhas/parasitologia
Colapso da Colônia/parasitologia
Resistência à Doença
Asseio Animal
Interações Hospedeiro-Parasita
Redes e Vias Metabólicas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175573



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