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Pesquisa : G01.249.335 [Categoria DeCS]
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  1 / 22171 MEDLINE  
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[PMID]:28454775
[Au] Autor:Eggert F; Kulikov K; Domnick C; Leifels P; Kath-Schorr S
[Ad] Endereço:LIMES Institute, Chemical Biology & Medicinal Chemistry Unit, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany.
[Ti] Título:Iluminated by foreign letters - Strategies for site-specific cyclopropene modification of large functional RNAs via in vitro transcription.
[So] Source:Methods;120:17-27, 2017 May 01.
[Is] ISSN:1095-9130
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The synthesis of sequence-specifically modified long RNA molecules, which cannot entirely be prepared via solid phase synthesis methods is experimentally challenging. We are using a new approach based on an expanded genetic alphabet preparing site-specifically modified RNA molecules via standard in vitro transcription. In this report, the site-specific labeling of functional RNAs, in particular ribozymes and a long non-coding RNA with cyclopropene moieties, is presented. We provide detailed instructions for RNA labeling via in vitro transcription and include required analytical methods to verify production and identity of the transcript. We further present post-transcriptional inverse electron demand Diels-Alder cycloaddition reactions on the cyclopropene-modified sequences and discuss applications of the genetic alphabet expansion transcription for in vitro preparation of labeled functional RNAs with complex foldings. In detail, the glmS and CPEB3 ribozymes were site-specifically decorated with methyl cyclopropene moieties using the unnatural TPT3 triphosphate and were proven to be still functional. In addition, the structurally complex A region of the Xist lncRNA (401nt) was site-specifically modified with methyl cyclopropene and detected by fluorescence after cycloaddition reaction with a tetrazine-BODIPY conjugate.
[Mh] Termos MeSH primário: Reação de Cicloadição/métodos
Ciclopropanos/química
RNA Catalítico/química
RNA Longo não Codificante/química
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Elétrons
Corantes Fluorescentes/química
Técnicas In Vitro/métodos
Nucleotídeos/química
Processamento Pós-Transcricional do RNA
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopropanes); 0 (Fluorescent Dyes); 0 (Nucleotides); 0 (RNA, Catalytic); 0 (RNA, Long Noncoding); J6UJO23JGU (1-methylcyclopropene)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  2 / 22171 MEDLINE  
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[PMID]:28987407
[Au] Autor:Shao HY; Wu MH; Deng F; Xu G; Liu N; Li X; Tang L
[Ad] Endereço:School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, PR China; Shanghai Environmental Monitoring Center, Shanghai, 200235, PR China.
[Ti] Título:Electron beam irradiation induced degradation of antidepressant drug fluoxetine in water matrices.
[So] Source:Chemosphere;190:184-190, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With the development of psychiatric disorder in the current society, abuse of antidepressant drug fluoxetine (FLX) has made such compound an emerging contaminant in natural waters, and causes endocrine systems disturbance on some aquatic species. Herein, an efficient advanced oxidation process (AOP), electron beam irradiation was carried out to investigate the decomposition characteristics of such novel environmental pollutant, including the effects of initial concentration, pH, radical scavengers and anions. The results showed that FLX degradation followed pseudo-first-order kinetics. The degradation rate and dose constant decreased with increasing initial FLX concentration; and G-values elevated with the increase of initial concentration but reduced with increase of absorbed dose. Acidic condition was more conducive to FLX destruction than neutral and alkaline. The radical scavenger experiments indicated OH was the main reactive species for the decomposition of FLX, while the reductive species e and H played an adjuvant role. The presence of anions slightly decreased or even no impact on FLX degradation rate. Various water matrices influenced degradation processes of FLX. Experimental results suggested radiolytic degradation showed the best performance in pure water rather than natural water no matter with filtration or not. Moreover, with the occurrence of defluorination and dealkylation during degradation process, some organic and inorganic intermediates were detected, and the possible degradation mechanisms and pathways of FLX were proposed.
[Mh] Termos MeSH primário: Fluoxetina/efeitos da radiação
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Ânions/farmacologia
Antidepressivos/efeitos da radiação
Elétrons
Fluoxetina/química
Cinética
Oxirredução
Poluentes Químicos da Água/química
Poluentes Químicos da Água/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anions); 0 (Antidepressive Agents); 0 (Water Pollutants, Chemical); 01K63SUP8D (Fluoxetine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  3 / 22171 MEDLINE  
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[PMID]:28742061
[Au] Autor:Poppinga D; Halbur J; Lemmer S; Delfs B; Harder D; Looe HK; Poppe B
[Ad] Endereço:University Clinic for Medical Radiation Physics, Medical Campus Pius-Hospital, Carl von Ossietzky University, Oldenburg, Germany.
[Ti] Título:Test study of boron nitride as a new detector material for dosimetry in high-energy photon beams.
[So] Source:Phys Med Biol;62(18):N436-N444, 2017 Sep 05.
[Is] ISSN:1361-6560
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this test study is to check whether boron nitride (BN) might be applied as a detector material in high-energy photon-beam dosimetry. Boron nitride exists in various crystalline forms. Hexagonal boron nitride (h-BN) possesses high mobility of the electrons and holes as well as a high volume resistivity, so that ionizing radiation in the clinical range of the dose rate can be expected to produce a measurable electrical current at low background current. Due to the low atomic numbers of its constituents, its density (2.0 g cm ) similar to silicon and its commercial availability, h-BN appears as possibly suitable for the dosimetry of ionizing radiation. Five h-BN plates were contacted to triaxial cables, and the detector current was measured in a solid-state ionization chamber circuit at an applied voltage of 50 V. Basic dosimetric properties such as formation by pre-irradiation, sensitivity, reproducibility, linearity and temporal resolution were measured with 6 MV photon irradiation. Depth dose curves at quadratic field sizes of 10 cm and 40 cm were measured and compared to ionization chamber measurements. After a pre-irradiation with 6 Gy, the devices show a stable current signal at a given dose rate. The current-voltage characteristic up to 400 V shows an increase in the collection efficiency with the voltage. The time-resolved detector current behavior during beam interrupts is comparable to diamond material, and the background current is negligible. The measured percentage depth dose curves at 10 cm × 10 cm field size agreed with the results of ionization chamber measurements within ±2%. This is a first study of boron nitride as a detector material for high-energy photon radiation. By current measurements on solid ionization chambers made from boron nitride chips we could demonstrate that boron nitride is in principle suitable as a detector material for high-energy photon-beam dosimetry.
[Mh] Termos MeSH primário: Compostos de Boro/química
Teste de Materiais
Fótons/uso terapêutico
Radiometria/instrumentação
Radiometria/métodos
Radioterapia de Alta Energia/instrumentação
[Mh] Termos MeSH secundário: Diamante/química
Elétrons
Reprodutibilidade dos Testes
Silício/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Boron Compounds); 2U4T60A6YD (boron nitride); 7782-40-3 (Diamond); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1088/1361-6560/aa81f7


  4 / 22171 MEDLINE  
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[PMID]:29410988
[Au] Autor:Ribar A; Huber SE; Smialek MA; Tanzer K; Neustetter M; Schürmann R; Bald I; Denifl S
[Ad] Endereço:Institute for Ion Physics and Applied Physics and Center of Molecular Biosciences Innsbruck, Leopold Franzens University of Innsbruck, Technikerstr. 25, 6020 Innsbruck, Austria. stephan.denifl@uibk.ac.at.
[Ti] Título:Hydroperoxyl radical and formic acid formation from common DNA stabilizers upon low energy electron attachment.
[So] Source:Phys Chem Chem Phys;20(8):5578-5585, 2018 Feb 21.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:2-Amino-2-(hydroxymethyl)-1,3-propanediol (TRIS) and ethylenediaminetetraacetic acid (EDTA) are key components of biological buffers and are frequently used as DNA stabilizers in irradiation studies. Such surface or liquid phase studies are done with the aim to understand the fundamental mechanisms of DNA radiation damage and to improve cancer radiotherapy. When ionizing radiation is used, abundant secondary electrons are formed during the irradiation process, which are able to attach to the molecular compounds present on the surface. In the present study we experimentally investigate low energy electron attachment to TRIS and methyliminodiacetic acid (MIDA), an analogue of EDTA, supported by quantum chemical calculations. The most prominent dissociation channel for TRIS is through hydroperoxyl radical formation, whereas the dissociation of MIDA results in the formation of formic and acetic acid. These compounds are well-known to cause DNA modifications, like strand breaks. The present results indicate that buffer compounds may not have an exclusive protecting effect on DNA as suggested previously.
[Mh] Termos MeSH primário: DNA/química
Elétrons
Formiatos/síntese química
Peróxidos/síntese química
Teoria Quântica
[Mh] Termos MeSH secundário: Formiatos/química
Radicais Livres/síntese química
Radicais Livres/química
Conformação de Ácido Nucleico
Peróxidos/química
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Formates); 0 (Free Radicals); 0 (Peroxides); 0YIW783RG1 (formic acid); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07697e


  5 / 22171 MEDLINE  
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[PMID]:29444032
[Au] Autor:Zhang Z; Huerta-Viga A; Tan HS
[Ti] Título:Two-dimensional electronic-Raman spectroscopy.
[So] Source:Opt Lett;43(4):939-942, 2018 Feb 15.
[Is] ISSN:1539-4794
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We present a new technique, two-dimensional electronic-Raman spectroscopy (2DER), which combines femtosecond stimulated Raman spectroscopy and a pulse-shaper-assisted 2D spectroscopic scheme for the actinic pump. The 2DER spectrum presents the initial actinic excitation wavelength with nanometer spectral resolution in the first axis and the detected stimulated Raman spectra in the second axis. We measured the correlation of the electronic and vibrational states in the photosynthetic accessory pigment ß-carotene and reveal its photoexcited state manifold.
[Mh] Termos MeSH primário: Elétrons
Análise Espectral Raman/métodos
[Mh] Termos MeSH secundário: beta Caroteno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
01YAE03M7J (beta Carotene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1364/OL.43.000939


  6 / 22171 MEDLINE  
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[PMID]:29327017
[Au] Autor:Kai T; Yokoya A; Ukai M; Fujii K; Toigawa T; Watanabe R
[Ad] Endereço:Nuclear Science and Engineering Center, Japan Atomic Energy Agency, 2-4 Shirane Shirakata, Tokai-mura, Naka-gun, Ibaraki, 319-1195, Japan.
[Ti] Título:A significant role of non-thermal equilibrated electrons in the formation of deleterious complex DNA damage.
[So] Source:Phys Chem Chem Phys;20(4):2838-2844, 2018 Jan 24.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although most of the radiation damage to genomic DNA could be rendered harmless using repair enzymes in a living cell, a certain fraction of the damage is persistent resulting in serious genetic effects, such as mutation induction. In order to understand the mechanisms of the deleterious DNA damage formation in terms of its earliest physical stage at the radiation track end, dynamics of low energy electrons and their thermalization processes around DNA molecules were investigated using a dynamic Monte Carlo code. The primary incident (1 keV) electrons multiply collide within 1 nm (equivalent to three DNA-base-pairs, 3bp) and generate secondary electrons which show non-Gaussian and non-thermal equilibrium distributions within 300 fs. On the other hand, the secondary electrons are mainly distributed within approximately 10 nm from their parent cations although approximately 5% of the electrons are localized within 1 nm of the cations owing to the interaction of their Coulombic fields. The mean electron energy is 0.7 eV; however, more than 10% of the electrons fall into a much lower-energy region than 0.1 eV at 300 fs. These results indicate that pre-hydrated electrons are formed from the extremely decelerated electrons over a few nm from the cations. DNA damage sites comprising multiple nucleobase lesions or single strand breaks can therefore be formed by multiple collisions of these electrons within 3bp. This multiple damage site is hardly processed by base excision repair enzymes. However, pre-hydrated electrons can also be produced resulting in an additional base lesion (or a strand break) more than 3bp away from the multi-damage site. These damage sites may be finally converted into a double strand break (DSB) when base excision enzymes process the additional base lesions. This DSB includes another base lesion(s) at their termini, and may introduce miss-rejoining by DSB repair enzymes, and hence may result in biological effects such as mutation in surviving cells.
[Mh] Termos MeSH primário: Dano ao DNA
DNA/metabolismo
[Mh] Termos MeSH secundário: DNA/química
Reparo do DNA
Elétrons
Método de Monte Carlo
Termodinâmica
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06903k


  7 / 22171 MEDLINE  
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[PMID]:28448985
[Au] Autor:Elsayad K; Susek KH; Eich HT
[Ti] Título:Total Skin Electron Beam Therapy as Part of Multimodal Treatment Strategies for Primary Cutaneous T-Cell Lymphoma.
[So] Source:Oncol Res Treat;40(5):244-252, 2017.
[Is] ISSN:2296-5262
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Total-skin electron beam therapy (TSEBT) is one of most effective treatments that has been used for cutaneous T-cell lymphoma. Low-dose TSEBT regimens (10-12 Gy) appear to be an effective alternative to conventional-dose TSEBT (30-36 Gy), yielding short-term remission of cutaneous manifestations with minimal toxicity. TSEBT can be administered to patients any time after a diagnosis of mycosis fungoides (MF). Patients requiring rapid relief from cutaneous lesions or symptoms may particularly benefit from TSEBT as an initial therapy. Radiotherapy (RT) dose, boost radiation delivery, maintenance treatment, and radiation tolerability may enhance remission rates and improve relapse-free survival following TSEBT. In addition, salvage local RT or TSEBT may be safely applied with high effectiveness. In this review, we focus on the use of TSEBT in patients with several forms of primary cutaneous T-cell lymphoma, and highlight the potential of low-dose TSEBT as part of a promising therapeutic approach.
[Mh] Termos MeSH primário: Elétrons/uso terapêutico
Linfoma Cutâneo de Células T/patologia
Linfoma Cutâneo de Células T/terapia
Lesões por Radiação/prevenção & controle
Radioterapia de Alta Energia/métodos
Neoplasias Cutâneas/patologia
Neoplasias Cutâneas/terapia
[Mh] Termos MeSH secundário: Terapia Combinada/efeitos adversos
Terapia Combinada/métodos
Relação Dose-Resposta à Radiação
Elétrons/efeitos adversos
Medicina Baseada em Evidências
Feminino
Seres Humanos
Masculino
Lesões por Radiação/etiologia
Dosagem Radioterapêutica
Radioterapia de Alta Energia/efeitos adversos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1159/000475634


  8 / 22171 MEDLINE  
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[PMID]:29238765
[Au] Autor:Varade V; Markus T; Vankayala K; Friedman N; Sheves M; Waldeck DH; Naaman R
[Ad] Endereço:Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel. ron.naaman@weizmann.ac.il.
[Ti] Título:Bacteriorhodopsin based non-magnetic spin filters for biomolecular spintronics.
[So] Source:Phys Chem Chem Phys;20(2):1091-1097, 2018 Jan 03.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We discuss spin injection and spin valves, which are based on organic and biomolecules, that offer the possibility to overcome some of the limitations of solid-state devices, which are based on ferromagnetic metal electrodes. In particular, we discuss spin filtering through bacteriorhodopsin in a solid state biomolecular spin valve that is based on the chirality induced spin selectivity (CISS) effect and shows a magnetoresistance of ∼2% at room temperature. The device is fabricated using a layer of bacteriorhodopsin (treated with n-octyl-thioglucoside detergent: OTG-bR) that is adsorbed on a cysteamine functionalized gold electrode and capped with a magnesium oxide layer as a tunneling barrier, upon which a Ni top electrode film is placed and used as a spin analyzer. The bR based spin valves show an antisymmetric magnetoresistance response when a magnetic field is applied along the direction of the current flow, whereas they display a positive symmetric magnetoresistance curve when a magnetic field is applied perpendicular to the current direction.
[Mh] Termos MeSH primário: Bacteriorodopsinas/química
Imãs
[Mh] Termos MeSH secundário: Eletrodos
Elétrons
Ouro
Campos Magnéticos
Tioglucosídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Thioglucosides); 53026-44-1 (Bacteriorhodopsins); 7440-57-5 (Gold); 85618-21-9 (n-octyl-beta-D-thioglucopyranoside)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06771b


  9 / 22171 MEDLINE  
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[PMID]:29184919
[Au] Autor:Luo Z; Friscic T; Khaliullin RZ
[Ad] Endereço:Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montreal, QC H3A 0B8, Canada. rustam@khaliullin.com.
[Ti] Título:Why pregnenolone and progesterone, two structurally similar steroids, exhibit remarkably different cocrystallization with aromatic molecules.
[So] Source:Phys Chem Chem Phys;20(2):898-904, 2018 Jan 03.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Selective binding of steroid molecules is of paramount importance for designing drugs that can target the biological pathways of only individual steroids. From this perspective, it is remarkable that progesterone (PRO) and pregnenolone (PRE), two structurally similar steroids, demonstrate a dramatically different propensity to interact with aromatic molecules. It has been recently reported that, in solid-state cocrystallization, PRO forms cocrystals with a wide variety of aromatic systems whereas PRE cocrystallizes only with a few. In this work, a simple yet effective computational procedure was developed to explain the fundamental origins of this surprising phenomenon. This procedure enables a direct comparison of the strength of intermolecular binding in the structurally similar cocrystals of PRO and PRE by generating experimentally inaccessible meta-stable cocrystals of PRE that closely resemble those observed for PRO. Direct comparative analysis shows that interactions between the α-face of the steroid and the π-electrons of aromatic molecules, the focus of previous studies, are not sufficiently different to explain the cocrystallization behavior of PRO and PRE. Instead, the observed difference is attributed to the different stabilities of the cocrystals relative to their pure components: organic and steroid crystals. It is calculated that the cocrystallization process is thermodynamically favorable in the case of PRO and unfavorable in the case of PRE. Furthermore, strong hydrogen bonds in the pure PRE crystal appear to be the major factor that makes the cocrystallization of PRE energetically unfavorable for a wide range of aromatic molecules. The fundamental analysis performed in this work has important practical implications for designing new steroid-containing crystals, selective biomolecular steroid receptors, and steroid-specific drugs. It suggests that a strategy for the selective binding of steroids should focus primarily on tuning the strength of hydrogen bonding.
[Mh] Termos MeSH primário: Ligações de Hidrogênio
Pregnenolona/química
Progesterona/química
[Mh] Termos MeSH secundário: Cristalização
Cristalografia
Desenho de Drogas
Elétrons
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4G7DS2Q64Y (Progesterone); 73R90F7MQ8 (Pregnenolone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06828j


  10 / 22171 MEDLINE  
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[PMID]:28739446
[Au] Autor:Panneerselvam S; Shehzad A; Mueller-Dieckmann J; Wilmanns M; Bocola M; Davari MD; Schwaneberg U
[Ad] Endereço:HASYLAB, DESY, Notkestrasse 85, 22603 Hamburg, Germany.
[Ti] Título:Crystallographic insights into a cobalt (III) sepulchrate based alternative cofactor system of P450 BM3 monooxygenase.
[So] Source:Biochim Biophys Acta;1866(1):134-140, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:P450 BM3 is a multi-domain heme-containing soluble bacterial monooxygenase. P450 BM3 and variants are known to oxidize structurally diverse substrates. Crystal structures of individual domains of P450 BM3 are available. However, the spatial organization of the full-length protein is unknown. In this study, crystal structures of the P450 BM3 M7 heme domain variant with and without cobalt (III) sepulchrate are reported. Cobalt (III) sepulchrate acts as an electron shuttle in an alternative cofactor system employing zinc dust as the electron source. The crystal structure shows a binding site for the mediator cobalt (III) sepulchrate at the entrance of the substrate access channel. The mediator occupies an unusual position which is far from the active site and distinct from the binding of the natural redox partner (FAD/NADPH binding domain).
[Mh] Termos MeSH primário: Bacillus megaterium/química
Proteínas de Bactérias/química
Cobalto/química
Coenzimas/química
Sistema Enzimático do Citocromo P-450/química
Elétrons
Heme/química
NADPH-Ferri-Hemoproteína Redutase/química
NADP/química
[Mh] Termos MeSH secundário: Bacillus megaterium/enzimologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Domínio Catalítico
Clonagem Molecular
Cobalto/metabolismo
Coenzimas/metabolismo
Cristalografia por Raios X
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Heme/metabolismo
Modelos Moleculares
NADP/metabolismo
NADPH-Ferri-Hemoproteína Redutase/genética
NADPH-Ferri-Hemoproteína Redutase/metabolismo
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
Zinco/química
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Coenzymes); 0 (Recombinant Proteins); 3G0H8C9362 (Cobalt); 42VZT0U6YR (Heme); 53-59-8 (NADP); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.6.2.4 (NADPH-Ferrihemoprotein Reductase); EC 1.6.2.4 (flavocytochrome P450 BM3 monoxygenases); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE



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