Base de dados : MEDLINE
Pesquisa : G01.249.467 [Categoria DeCS]
Referências encontradas : 1266 [refinar]
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[PMID]:28741678
[Au] Autor:Hu W; Li W; Chen J
[Ad] Endereço:Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
[Ti] Título:Recent advances of microbial breeding via heavy-ion mutagenesis at IMP.
[So] Source:Lett Appl Microbiol;65(4):274-280, 2017 Oct.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nowadays, the value of heavy-ion mutagenesis has been accepted as a novel powerful mutagen technique to generate new microbial mutants due to its high linear energy transfer and high relative biological effectiveness. This paper briefly reviews recent progress in developing a more efficient mutagenesis technique for microbial breeding using heavy-ion mutagenesis, and also presents the outline of the beam line for microbial breeding in Heavy Ion Research Facility of Lanzhou. Then, new insights into microbial biotechnology via heavy-ion mutagenesis are also further explored. We hope that our concerns will give deep insight into microbial breeding biotechnology via heavy-ion mutagenesis. We also believe that heavy-ion mutagenesis breeding will greatly contribute to the progress of a comprehensive study industrial strain engineering for bioindustry in the future. SIGNIFICANCE AND IMPACT OF THE STUDY: There is currently a great interest in developing rapid and diverse microbial mutation tool for strain modification. Heavy-ion mutagenesis has been proved as a powerful technology for microbial breeding due to its broad spectrum of mutation phenotypes with high efficiency. In order to deeply understand heavy-ion mutagenesis technology, this paper briefly reviews recent progress in microbial breeding using heavy-ion mutagenesis at IMP, and also presents the outline of the beam line for microbial breeding in Heavy Ion Research Facility of Lanzhou (HIRFL) as well as new insights into microbial biotechnology via heavy-ion mutagenesis. Thus, this work can provide the guidelines to promote the development of novel microbial biotechnology cross-linking heavy-ion mutagenesis breeding that could make breeding process more efficiently in the future.
[Mh] Termos MeSH primário: Aspergillus/genética
Clostridium/genética
Íons Pesados
Transferência Linear de Energia/fisiologia
Microalgas/genética
Mutagênese/genética
Trichoderma/genética
[Mh] Termos MeSH secundário: Aspergillus/efeitos da radiação
Cruzamento
Clostridium/efeitos da radiação
Microalgas/efeitos da radiação
Mutação/genética
Fenótipo
Radiação Ionizante
Trichoderma/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12780


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[PMID]:28910671
[Au] Autor:Bláha P; Koshlan NA; Koshlan IV; Petrova DV; Bogdanova YV; Govorun RD; Múcka V; Krasavin EA
[Ad] Endereço:Laboratory of Radiation Biology, Joint Institute for Nuclear Research, Joliot--Curie 6, 141980, Dubna, Moscow Region, Russia; Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Brehová 7, 11519, Prague 1, Czech Republic. Electronic address: pavel.blahax@gmail
[Ti] Título:Delayed effects of accelerated heavy ions on the induction of HPRT mutations in V79 hamster cells.
[So] Source:Mutat Res;803-805:35-41, 2017 Oct.
[Is] ISSN:1873-135X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fundamental research on the harmful effects of ionizing radiation on living cells continues to be of great interest. Recently, priority has been given to the study of high-charge and high-energy (HZE) ions that comprise a substantial part of the galactic cosmic ray (GCR) spectra that would be encountered during long-term space flights. Moreover, predictions of the delayed genetic effects of high linear energy transfer (LET) exposure is becoming more important as heavy ion therapy use is increasing. This work focuses mainly on the basic research on the delayed effects of HZE ions on V79 Chinese hamster cells, with emphasis on the induction of HPRT mutations after prolonged expression times (ET). The research was conducted under various irradiation conditions with accelerated ions O (E=35.2MeV/n), Ne (E=47.7MeV/n and 51.8MeV/n), and B (E=32.4MeV/n), with LET in the range from 49 to 149 keV/µm and with Co γ-rays. The HPRT mutant fractions (MF) were detected in irradiated cells in regular intervals during every cell culture recultivation (every 3days) up to approximately 40days (70-80 generations) after irradiation. The MF maximum was reached at different ET depending on ionizing radiation characteristics. The position of the maximum was shifting towards longer ET with increasing LET. We speculate that the delayed mutations are created de novo and that they are the manifestation of genomic instability. Although the exact mechanisms involved in genomic instability initiation are yet to be identified, we hypothesize that differences in induction of delayed mutations by radiations with various LET values are related to variations in energy deposition along the particle track. A dose dependence of mutation yield is discussed as well.
[Mh] Termos MeSH primário: Raios gama
Íons Pesados/efeitos adversos
Hipoxantina Fosforribosiltransferase/genética
Mutação
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cricetinae
Cricetulus
Relação Dose-Resposta à Radiação
Fibroblastos/efeitos da radiação
Instabilidade Genômica/efeitos da radiação
Transferência Linear de Energia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE


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[PMID]:28683078
[Au] Autor:Turker MS; Grygoryev D; Lasarev M; Ohlrich A; Rwatambuga FA; Johnson S; Dan C; Eckelmann B; Hryciw G; Mao JH; Snijders AM; Gauny S; Kronenberg A
[Ad] Endereço:Oregon Institute of Occupational Health Sciences, Oregon Health & Science University, Portland, Oregon, United States of America.
[Ti] Título:Simulated space radiation-induced mutants in the mouse kidney display widespread genomic change.
[So] Source:PLoS One;12(7):e0180412, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Exposure to a small number of high-energy heavy charged particles (HZE ions), as found in the deep space environment, could significantly affect astronaut health following prolonged periods of space travel if these ions induce mutations and related cancers. In this study, we used an in vivo mutagenesis assay to define the mutagenic effects of accelerated 56Fe ions (1 GeV/amu, 151 keV/µm) in the mouse kidney epithelium exposed to doses ranging from 0.25 to 2.0 Gy. These doses represent fluences ranging from 1 to 8 particle traversals per cell nucleus. The Aprt locus, located on chromosome 8, was used to select induced and spontaneous mutants. To fully define the mutagenic effects, we used multiple endpoints including mutant frequencies, mutation spectrum for chromosome 8, translocations involving chromosome 8, and mutations affecting non-selected chromosomes. The results demonstrate mutagenic effects that often affect multiple chromosomes for all Fe ion doses tested. For comparison with the most abundant sparsely ionizing particle found in space, we also examined the mutagenic effects of high-energy protons (1 GeV, 0.24 keV/µm) at 0.5 and 1.0 Gy. Similar doses of protons were not as mutagenic as Fe ions for many assays, though genomic effects were detected in Aprt mutants at these doses. Considered as a whole, the data demonstrate that Fe ions are highly mutagenic at the low doses and fluences of relevance to human spaceflight, and that cells with considerable genomic mutations are readily induced by these exposures and persist in the kidney epithelium. The level of genomic change produced by low fluence exposure to heavy ions is reminiscent of the extensive rearrangements seen in tumor genomes suggesting a potential initiation step in radiation carcinogenesis.
[Mh] Termos MeSH primário: Cromossomos/efeitos da radiação
Epitélio/efeitos da radiação
Radioisótopos de Ferro/efeitos adversos
Rim/efeitos da radiação
Fótons/efeitos adversos
Translocação Genética/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Carcinogênese/efeitos da radiação
Cromossomos/química
Radiação Cósmica/efeitos adversos
Feminino
Loci Gênicos/efeitos da radiação
Íons Pesados
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Simulação de Ambiente Espacial
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iron Radioisotopes)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180412


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[PMID]:28650980
[Au] Autor:Hu W; Li W; Chen H; Liu J; Wang S; Chen J
[Ad] Endereço:Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou city, Gansu Province, China.
[Ti] Título:Changes in transcript levels of starch hydrolysis genes and raising citric acid production via carbon ion irradiation mutagenesis of Aspergillus niger.
[So] Source:PLoS One;12(6):e0180120, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filamentous ascomycete Aspergillus niger is well known for its ability to accumulate citric acid for the hydrolysis of starchy materials. To improve citric acid productivity, heavy ion beam mutagenesis was utilized to produce mutant A.niger strains with enhanced production of citric acid in this work. It was demonstrated that a mutant HW2 with high concentration of citric acid was isolated after carbon ion irradiation with the energy of 80Mev/µ, which was obvious increase higher than the original strain from liquefied corn starch as a feedstock. More importantly, with the evidence from the expression profiles of key genes and enzyme activity involved in the starch hydrolysis process between original strain and various phenotype mutants, our results confirmed that different transcript levels of key genes involving in starch hydrolysis process between original strain and mutants could be a significant contributor to different citric acid concentration in A.niger, such as, amyR and glaA, which therefore opened a new avenue for constructing genetically engineered A.niger mutants for high-yield citric acid accumulation in the future. As such, this work demonstrated that heavy ion beam mutagenesis presented an efficient alternative strategy to be developed to generate various phenotype microbe species mutants for functional genes research.
[Mh] Termos MeSH primário: Aspergillus niger/genética
Aspergillus niger/metabolismo
Ácido Cítrico/metabolismo
Genes Fúngicos
Mutagênese
Amido/metabolismo
[Mh] Termos MeSH secundário: Aspergillus niger/efeitos da radiação
Carbono
Fermentação
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Genes Fúngicos/efeitos da radiação
Glucana 1,4-alfa-Glucosidase/genética
Glucana 1,4-alfa-Glucosidase/metabolismo
Íons Pesados
Hidrólise
Fenótipo
Transativadores/genética
Transativadores/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Trans-Activators); 0 (amyR protein, Aspergillus); 2968PHW8QP (Citric Acid); 7440-44-0 (Carbon); 9005-25-8 (Starch); EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180120


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[PMID]:28418703
[Au] Autor:Vignoli Muniz GS; Mejía CF; Martinez R; Auge B; Rothard H; Domaracka A; Boduch P
[Ad] Endereço:1 Centre de Recherche sur les Ions, les Matériaux et la Photonique, Normandie Univ , ENSICAEN, UNICAEN, CEA, CNRS, CIMAP, Caen, France .
[Ti] Título:Radioresistance of Adenine to Cosmic Rays.
[So] Source:Astrobiology;17(4):298-308, 2017 Apr.
[Is] ISSN:1557-8070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The presence of nucleobases in carbonaceous meteorites on Earth is an indication of the existence of this class of molecules in outer space. However, space is permeated by ionizing radiation, which can have damaging effects on these molecules. Adenine is a purine nucleobase that amalgamates important biomolecules such as DNA, RNA, and ATP. Adenine has a unique importance in biochemistry and therefore life. The aim of this work was to study the effects of cosmic ray analogues on solid adenine and estimate its survival when exposed to corpuscular radiation. Adenine films were irradiated at GANIL (Caen, France) and GSI (Darmstadt, Germany) by 820 MeV Kr , 190 MeV Ca , 92 MeV Xe , and 12 MeV C ion beams at low temperature. The evolution of adenine molecules under heavy ion irradiation was studied by IR absorption spectroscopy as a function of projectile fluence. It was found that the adenine destruction cross section (σ ) follows an electronic stopping power (S ) power law under the form: CS ; C is a constant, and the exponential n is a dimensionless quantity. Using the equation above to fit our results, we determined σ = 4 × 10 S , with S in kiloelectronvolts per micrometer (keV µm ). New IR absorption bands arise under irradiation of adenine and can be attributed to HCN, CN , C H N , CH CN, and (CH ) CNC. These findings may help to understand the stability and chemistry related to complex organic molecules in space. The half-life of solid adenine exposed to the simulated interstellar medium cosmic ray flux was estimated as (10 ± 8) × 10 years. Key Words: Heavy ions-Infrared spectroscopy-Astrochemistry-Cosmic rays-Nucleobases-Adenine. Astrobiology 17, 298-308.
[Mh] Termos MeSH primário: Adenina/química
Radiação Cósmica
[Mh] Termos MeSH secundário: Íons Pesados
Gelo
Espectrofotometria Infravermelho
Temperatura Ambiente
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ice); 059QF0KO0R (Water); JAC85A2161 (Adenine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1089/ast.2016.1488


  6 / 1266 MEDLINE  
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[PMID]:28299960
[Au] Autor:Jung IJ; Hwang JE; Han SM; Kim DS; Ahn JW; Choi HI; Kwon SJ; Kang SY; Kim JB
[Ad] Endereço:a Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute , Jeongeup , Jeollabuk , Republic of Korea.
[Ti] Título:Molecular dissection of the response of the rice Systemic Acquired Resistance Deficient 1 (SARD1) gene to different types of ionizing radiation.
[So] Source:Int J Radiat Biol;93(7):717-725, 2017 Jul.
[Is] ISSN:1362-3095
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ionizing radiation, jasmonic acid (JA) or salicylic acid (SA). MATERIALS AND METHODS: Rice seeds exposed to two types of ionizing radiations (gamma ray [GR] and ion beam [IB]) were analyzed by quantitative reverse transcription PCR (qRT-PCR) to identify the genes that are altered in response to ionizing radiation. Then, OsSARD1-overexpressing homozygous Arabidopsis plants were generated to assess the effects of OsSARD1 in the response to irradiation. The phenotypes of these transgenic plants, as well as control plants, were monitored after GR irradiation at doses of 200 and 300 Gray (Gy). RESULTS: The OsSARD1 transcript was strongly downregulated after exposure to GR and IB irradiation. Previous phylogenetic analysis showed that the Arabidopsis SARD1 (AtSARD1) protein is closely related to Arabidopsis calmodulin-binding protein 60g (AtCBP60g), which is known to be required for activation of SA biosynthesis. In this study, phylogenetic analysis showed that OsSARD1 was grouped with AtSARD1. The OsSARD1 gene was induced after exposure to SA and JA. The biological phenotype of OsSARD1-overexpressing Arabidopsis plants was examined. OsSARD1-overexpressing plants displayed resistance to GR; in comparison with wild-type plants, the height and weight of OsSARD1-overexpressing plants were significantly greater after GR irradiation. In addition, OsSARD1 protein was abundantly accumulated in the nucleus. CONCLUSIONS: The results indicate that OsSARD1 plays an important role in the regulation of the defense responses to GR and IB irradiation and exhibits phytohormone induced expression.
[Mh] Termos MeSH primário: Proteínas de Ligação a Calmodulina/metabolismo
Regulação da Expressão Gênica de Plantas/fisiologia
Oryza/metabolismo
Oryza/efeitos da radiação
Proteínas de Plantas/metabolismo
Radiação Ionizante
Estresse Fisiológico/fisiologia
Estresse Fisiológico/efeitos da radiação
[Mh] Termos MeSH secundário: Mecanismos de Defesa
Relação Dose-Resposta à Radiação
Raios gama
Íons Pesados
Dose de Radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calmodulin-Binding Proteins); 0 (Plant Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1080/09553002.2017.1297901


  7 / 1266 MEDLINE  
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[PMID]:28243599
[Au] Autor:Wang SY; Bo YH; Zhou X; Chen JH; Li WJ; Liang JP; Xiao GQ; Wang YC; Liu J; Hu W; Jiang BL
[Ad] Endereço:Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou, Gansu 730000, China; Lanzhou University, 222 South Tianshui Road, Lanzhou, Gansu 730000, China.
[Ti] Título:Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered .
[So] Source:Biomed Res Int;2017:5373262, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heavy-ion irradiation technology has advantages over traditional methods of mutagenesis. Heavy-ion irradiation improves the mutation rate, broadens the mutation spectrum, and shortens the breeding cycle. However, few data are currently available regarding its effect on morphology and productivity. In this study, the influence of heavy-ion irradiation on when cultivated in approximately 10 L stirred-tank bioreactors was investigated. The specific productivity of the avermectin (AVM) B1a-producing mutant 147-G 8 increased notably, from 3885 to 5446 g/mL, approximately 1.6-fold, compared to the original strain. The mycelial morphology of the mutant fermentation processes was microscopically examined. Additionally, protein and metabolite identification was performed by using SDS-PAGE, 2- and 3-dimensional electrophoresis (2DE and 3DE). The results showed that negative regulation gene deletion of mutants led to metabolic process upregulating expression of protein and improving the productivity of an avermectin B1a. The results showed that the heavy-ion beam irradiation dose that corresponded to optimal production was well over the standard dose, at approximately 80 Gy at 220 AMeV (depending on the strain). This study provides reliable data and a feasible method for increasing AVM productivity in industrial processes.
[Mh] Termos MeSH primário: Engenharia Genética/métodos
Íons Pesados
Ivermectina/análogos & derivados
Streptomyces/metabolismo
Streptomyces/efeitos da radiação
[Mh] Termos MeSH secundário: Reatores Biológicos
Carbono/química
Eletroforese em Gel Bidimensional
Ivermectina/metabolismo
Cinética
Taxa de Mutação
Micélio/citologia
Micélio/efeitos da radiação
Streptomyces/genética
Streptomyces/crescimento & desenvolvimento
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
65195-55-3 (avermectin B(1)a); 70288-86-7 (Ivermectin); 7440-44-0 (Carbon)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1155/2017/5373262


  8 / 1266 MEDLINE  
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[PMID]:28206820
[Au] Autor:Jönsson KI; Wojcik A
[Ad] Endereço:1 School of Education and Environment, Kristianstad University , Kristianstad, Sweden .
[Ti] Título:Tolerance to X-rays and Heavy Ions (Fe, He) in the Tardigrade Richtersius coronifer and the Bdelloid Rotifer Mniobia russeola.
[So] Source:Astrobiology;17(2):163-167, 2017 Feb.
[Is] ISSN:1557-8070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to analyze tolerance to heavy ions in desiccated animals of the eutardigrade Richtersius coronifer and the bdelloid rotifer Mniobia russeola within the STARLIFE project. Both species were exposed to iron (Fe) and helium (He) ions at the Heavy Ion Medical Accelerator in Chiba (HIMAC) in Chiba, Japan, and to X-rays at the German Aerospace Center (DLR) in Cologne, Germany. Results show no effect of Fe and He on viability up to 7 days post-rehydration in both R. coronifer and M. russeola, while X-rays tended to reduce viability in R. coronifer at the highest doses. Mean egg production rate tended to decline with higher doses in R. coronifer for all radiation types, but the pattern was not statistically confirmed. In M. russeola, there was no such tendency for a dose response in egg production rate. These results confirm the previously reported high tolerance to high linear energy transfer (LET) radiation in tardigrades and show for the first time that bdelloid rotifers are also very tolerant to high-LET radiation. These animal phyla represent the most desiccation- and radiation-tolerant animals on Earth and provide excellent eukaryotic models for astrobiological research. Key Words: Tardigrada-Rotifera-Radiation tolerance-Heavy ions-X-rays. Astrobiology 17, 163-167.
[Mh] Termos MeSH primário: Íons Pesados
Hélio/química
Ferro/química
Rotíferos/efeitos da radiação
Tardígrados/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Óvulo/efeitos da radiação
Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
206GF3GB41 (Helium); E1UOL152H7 (Iron)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1089/ast.2015.1462


  9 / 1266 MEDLINE  
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[PMID]:28151694
[Au] Autor:Leuko S; Rettberg P
[Ad] Endereço:Astrobiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine , German Aerospace Center (DLR), Cologne, Germany .
[Ti] Título:The Effects of HZE Particles, γ and X-ray Radiation on the Survival and Genetic Integrity of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuae.
[So] Source:Astrobiology;17(2):110-117, 2017 Feb.
[Is] ISSN:1557-8070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three halophilic archaea, Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuae, have been exposed to different regimes of simulated outer space ionizing radiation. Strains were exposed to high-energy heavy ion (HZE) particles, namely iron and argon ions, as well as to γ radiation ( Co) and X-rays, and the survival and the genetic integrity of the 16S rRNA gene were evaluated. Exposure to 1 kGy of argon or iron ions at the Heavy Ion Medical Accelerator in Chiba (HIMAC) facility at the National Institute for Radiological Sciences (NIRS) in Japan did not lead to a detectable loss in viability; only after exposure to 2 kGy of iron ions a decline in survival was observed. Furthermore, a delay in growth was manifested following exposure to 2 kGy iron ions. DNA integrity of the 16S rRNA was not compromised up to 1 kGy, with the exception of Hcc. hamelinensis following exposure to argon particles. All three strains showed a high resistance toward X-rays (exposed at the DLR in Cologne, Germany), where Hcc. hamelinensis and Hcc. morrhuae displayed better survival compared to Hbt. salinarum NRC-1. In all three organisms the DNA damage increased in a dose-dependent manner. To determine a biological endpoint for survival following exposure to γ radiation, strains were exposed to up to 112 kGy at the Beta-Gamma-Service GmbH (BGS) in Germany. Although all strains were incubated for up to 4 months, only Hcc. hamelinensis and Hcc. morrhuae recovered from 6 kGy of γ radiation. In comparison, Hbt. salinarum NRC-1 did not recover. The 16S rRNA gene integrity stayed remarkably well preserved up to 48 kGy for both halococci. This research presents novel data on the survival and genetic stability of three halophilic archaea following exposure to simulated outer space radiation. Key Words: Halophilic archaea-Radiation-Survival. Astrobiology 17, 110-117.
[Mh] Termos MeSH primário: Raios gama
Halobacterium salinarum/genética
Halobacterium salinarum/efeitos da radiação
Halococcus/genética
Halococcus/efeitos da radiação
Íons Pesados
Viabilidade Microbiana/efeitos da radiação
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana
DNA Bacteriano/genética
Halobacterium salinarum/crescimento & desenvolvimento
Halococcus/crescimento & desenvolvimento
Fatores de Tempo
Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1089/ast.2015.1458


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[PMID]:28133985
[Au] Autor:Qin J; Li S; Zhang C; Gao DW; Li Q; Zhang H; Jin XD; Liu Y
[Ad] Endereço:1 Department of Radiotherapy, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
[Ti] Título:Apoptosis and injuries of heavy ion beam and x-ray radiation on malignant melanoma cell.
[So] Source:Exp Biol Med (Maywood);242(9):953-960, 2017 May.
[Is] ISSN:1535-3699
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aims to investigate the influence of high linear energy transfer (LET) heavy ion ( C ) and low LET X-ray radiation on apoptosis and related proteins of malignant melanoma on tumor-bearing mice under the same physical dosage. C57BL/6 J mice were burdened by tumors and randomized into three groups. These mice received heavy ion ( C ) and X-ray radiation under the same physical dosage, respectively; their weight and tumor volumes were measured every three days post-radiation. After 30 days, these mice were sacrificed. Then, median survival time was calculated and tumors on mice were proliferated. In addition, immunohistochemistry was carried out for apoptosis-related proteins to reflect the expression level. After tumor-bearing mice were radiated to heavy ion, median survival time improved and tumor volume significantly decreased in conjunction with the upregulated expression of pro-apoptosis factors, Bax and cytochrome C, and the downregulated expression of apoptosis-profilin (Bcl-2, Survivin) and proliferation-related proteins (proliferating cell nuclear antigen). The results indicated that radiation can promote the apoptosis of malignant melanoma cells and inhibit their proliferation. This case was more suitable for heavy ion ( C ). High LET heavy ion ( C ) radiation could significantly improve the killing ability for malignant melanoma cells by inducing apoptosis in tumor cells and inhibiting their proliferation. These results demonstrated that heavy ion ( C ) presented special advantages in terms of treating malignant melanoma. Impact statement Malignant melanoma is a malignant skin tumor derived from melanin cells, which has a high malignant degree and high fatality rate. In this study, proliferating cell nuclear antigen (PCNA) can induce the apoptosis of malignant melanoma cells and inhibit its proliferation, and its induction effect on apoptosis is significantly higher than low LET X-ray; hence, it is expected to overcome its lower sensitivity to radiation. This study can provide theoretical basis for clinical trials, in which malignant melanoma is treated by heavy ion ( C ), in order to accurately determine the clinical efficacy of heavy ion therapy. Clinical applications has revealed that local tumor control rate is high when heavy ion is used to treat malignant melanoma, indicating that heavy ion is an important direction in treating melanoma in the future.
[Mh] Termos MeSH primário: Apoptose/efeitos da radiação
Íons Pesados
Melanoma/patologia
Melanoma/radioterapia
Radiação
Radioterapia/métodos
Neoplasias Cutâneas/patologia
Neoplasias Cutâneas/radioterapia
Raios X
[Mh] Termos MeSH secundário: Animais
Proteínas Reguladoras de Apoptose/análise
Imuno-Histoquímica
Camundongos Endogâmicos C57BL
Análise de Sobrevida
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE
[do] DOI:10.1177/1535370216689827



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