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  1 / 10742 MEDLINE  
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[PMID]:28742061
[Au] Autor:Poppinga D; Halbur J; Lemmer S; Delfs B; Harder D; Looe HK; Poppe B
[Ad] Endereço:University Clinic for Medical Radiation Physics, Medical Campus Pius-Hospital, Carl von Ossietzky University, Oldenburg, Germany.
[Ti] Título:Test study of boron nitride as a new detector material for dosimetry in high-energy photon beams.
[So] Source:Phys Med Biol;62(18):N436-N444, 2017 Sep 05.
[Is] ISSN:1361-6560
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this test study is to check whether boron nitride (BN) might be applied as a detector material in high-energy photon-beam dosimetry. Boron nitride exists in various crystalline forms. Hexagonal boron nitride (h-BN) possesses high mobility of the electrons and holes as well as a high volume resistivity, so that ionizing radiation in the clinical range of the dose rate can be expected to produce a measurable electrical current at low background current. Due to the low atomic numbers of its constituents, its density (2.0 g cm ) similar to silicon and its commercial availability, h-BN appears as possibly suitable for the dosimetry of ionizing radiation. Five h-BN plates were contacted to triaxial cables, and the detector current was measured in a solid-state ionization chamber circuit at an applied voltage of 50 V. Basic dosimetric properties such as formation by pre-irradiation, sensitivity, reproducibility, linearity and temporal resolution were measured with 6 MV photon irradiation. Depth dose curves at quadratic field sizes of 10 cm and 40 cm were measured and compared to ionization chamber measurements. After a pre-irradiation with 6 Gy, the devices show a stable current signal at a given dose rate. The current-voltage characteristic up to 400 V shows an increase in the collection efficiency with the voltage. The time-resolved detector current behavior during beam interrupts is comparable to diamond material, and the background current is negligible. The measured percentage depth dose curves at 10 cm × 10 cm field size agreed with the results of ionization chamber measurements within ±2%. This is a first study of boron nitride as a detector material for high-energy photon radiation. By current measurements on solid ionization chambers made from boron nitride chips we could demonstrate that boron nitride is in principle suitable as a detector material for high-energy photon-beam dosimetry.
[Mh] Termos MeSH primário: Compostos de Boro/química
Teste de Materiais
Fótons/uso terapêutico
Radiometria/instrumentação
Radiometria/métodos
Radioterapia de Alta Energia/instrumentação
[Mh] Termos MeSH secundário: Diamante/química
Elétrons
Reprodutibilidade dos Testes
Silício/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Boron Compounds); 2U4T60A6YD (boron nitride); 7782-40-3 (Diamond); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1088/1361-6560/aa81f7


  2 / 10742 MEDLINE  
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[PMID]:28745371
[Au] Autor:Tang S; Ghazvini Zadeh EH; Kim B; Toomey NT; Bondar MV; Belfield KD
[Ad] Endereço:Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA.
[Ti] Título:Protein-induced fluorescence enhancement of two-photon excitable water-soluble diketopyrrolopyrroles.
[So] Source:Org Biomol Chem;15(31):6511-6519, 2017 Aug 09.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fluorescent contrast agents are important tools in cell biology and medical imaging due to their high sensitivity and relative availability. Diketopyrrolopyrrole (DPP) derivatives have been recently studied for applications in bioimaging, but certain drawbacks due to their inherent structure have stifled progress towards their widespread implementation. Aggregation caused quenching (ACQ) associated with π-π stacking in relatively rigid extended conjugation systems as well as hydrophobicity of previously reported DPPs make most unsuitable for biological imaging applications. Addressing these deficiencies, we report the synthesis and photophysical characterization of two new water-soluble diketopyrrolopyrole (DPP) probes that exhibit pronounced protein-induced fluorescence enhancement (PIFE) upon binding serum albumin protein. In vitro studies were also performed showing low cytotoxicity for the new DPP probes. Two-photon fluorescence microscopy (2PFM) images were obtained via excitation at 810 nm and emission in the NIR window of biological transparency, illustrating the potential of these compounds as nonlinear optical bioimaging probes.
[Mh] Termos MeSH primário: Corantes Fluorescentes/química
Imagem Óptica/métodos
Pirróis/química
Albumina Sérica/metabolismo
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Fluorescência
Corantes Fluorescentes/síntese química
Corantes Fluorescentes/metabolismo
Corantes Fluorescentes/toxicidade
Células HCT116
Seres Humanos
Microscopia de Fluorescência/métodos
Fótons
Ligação Proteica
Pirróis/síntese química
Pirróis/metabolismo
Pirróis/toxicidade
Solubilidade
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Pyrroles); 0 (Serum Albumin); 059QF0KO0R (Water)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob01397c


  3 / 10742 MEDLINE  
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[PMID]:28470530
[Au] Autor:Buttgereit A
[Ad] Endereço:Institute of Medical Biotechnology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. Andreas.Buttgereit@t-online.de.
[Ti] Título:Second Harmonic Generation Microscopy of Muscle Cell Morphology and Dynamics.
[So] Source:Methods Mol Biol;1601:229-241, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microscopy in combination with contrast-increasing dyes allows the visualization and analysis of organs, tissues, and various cells. Because of their better resolution, the development of confocal and laser microscopes enables the investigations of cell components, which are labeled with fluorescent dyes. The imaging of living cells on subcellular level (also in vivo) needs a labeling by gene transfection of GFP or similar labeled proteins. We present a method for visualization of cell structure in skeletal and heart muscle by label-free Second Harmonic Generation (SHG) microscopy and describe analytic methods for quantitative measurements of morphology and dynamics in skeletal muscle fibers.
[Mh] Termos MeSH primário: Fibras Musculares Esqueléticas/ultraestrutura
Miócitos Cardíacos/ultraestrutura
Microscopia de Geração do Segundo Harmônico/métodos
[Mh] Termos MeSH secundário: Animais
Corantes Fluorescentes/química
Proteínas de Fluorescência Verde/química
Imagem Tridimensional
Microscopia Confocal
Fibras Musculares Esqueléticas/química
Miócitos Cardíacos/química
Miosinas/química
Fótons
Sarcômeros/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 147336-22-9 (Green Fluorescent Proteins); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_18


  4 / 10742 MEDLINE  
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[PMID]:28464742
[Au] Autor:Holm AIS; Petersen JBB; Muren LP; Seiersen K; Borghammer P; Lukacova S
[Ad] Endereço:a Department of Medical Physics , Aarhus University Hospital , Aarhus , Denmark.
[Ti] Título:Functional image-guided dose escalation in gliomas using of state-of-the-art photon vs. proton therapy.
[So] Source:Acta Oncol;56(6):826-831, 2017 Jun.
[Is] ISSN:1651-226X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recurrences of glioma are usually local, suggesting the need for higher tumor dose. We investigated the boundaries for dose escalation of an F-fluoro-ethyl-tyrosine positron emission tomography defined target by intensity-modulated photon therapy (IMRT), volumetric modulated arc therapy (VMAT) and intensity-modulated proton therapy (IMPT). MATERIALS AND METHODS: Standard dose (60 Gy) and dose-escalated plans were calculated for seven patients using IMRT, VMAT and IMPT. The achieved boost dose, the dose to the organs at risk (OAR), the dose homogeneity (defined as overdose volume, ODV) and the ratio of the 30 Gy isodose curve and the boost volume (R30) were compared. The risk of radionecrosis was estimated using the ratio of the dose volume histograms of the brain (range 30-60 Gy). RESULTS: The mean boost dose was 77.1 Gy for IMRT, 79.2 Gy for VMAT and 85.1 GyE for IMPT. Compared with the standard plan, the ODV was unchanged and the R30 increased (17%) for IMRT. For VMAT, the ODV decreased (7%) and the R30 was unchanged whereas IMPT substantially decreased ODV (61%), R30 (22%), OAR doses as well as the risk of radionecrosis. CONCLUSIONS: Dose escalation can be achieved with IMRT, VMAT and IMPT while respecting normal tissue constraints, yet with IMPT being most favorable.
[Mh] Termos MeSH primário: Neoplasias Encefálicas/radioterapia
Glioma/radioterapia
Recidiva Local de Neoplasia/radioterapia
Fótons/uso terapêutico
Terapia com Prótons
Planejamento da Radioterapia Assistida por Computador/métodos
Radioterapia Guiada por Imagem/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Neoplasias Encefálicas/diagnóstico por imagem
Neoplasias Encefálicas/patologia
Feminino
Glioma/diagnóstico por imagem
Glioma/patologia
Seres Humanos
Processamento de Imagem Assistida por Computador/métodos
Masculino
Meia-Idade
Recidiva Local de Neoplasia/diagnóstico por imagem
Recidiva Local de Neoplasia/patologia
Órgãos em Risco/efeitos da radiação
Tomografia por Emissão de Pósitrons
Dosagem Radioterapêutica
Radioterapia de Intensidade Modulada/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1080/0284186X.2017.1285498


  5 / 10742 MEDLINE  
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[PMID]:28464733
[Au] Autor:Busch K; G Andersen A; Casares-Magaz O; Petersen JBB; Bentzen L; Thörnqvist S; Muren LP
[Ad] Endereço:a Department of Medical Physics , Aarhus University Hospital/Aarhus University , Aarhus , Denmark.
[Ti] Título:Evaluating the influence of organ motion during photon vs. proton therapy for locally advanced prostate cancer using biological models.
[So] Source:Acta Oncol;56(6):839-845, 2017 Jun.
[Is] ISSN:1651-226X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Proton therapy (PT) may have a normal tissue sparing potential when co-irradiating pelvic lymph nodes in patients with locally advanced prostate cancer, but may also be more sensitive towards organ motion in the pelvis. Building upon a previous study identifying motion-robust proton beam angles for pelvic irradiation, we aimed to evaluate the influence of organ motion for PT using biological models, and to compare this with contemporary photon-based RT. MATERIAL AND METHODS: Eight locally advanced prostate cancer patients with a planning CT (pCT) and 8-9 repeated CT scans (rCTs) were included. Two PT plans were created, one using two lateral opposed beams at gantry angles of 90°/270° and the other using two lateral oblique beams at 35°/325°; these were compared with volumetric modulated arc therapy (VMAT) plans. All plans were optimised on the pCT and subsequently re-calculated on each rCT (following rigid alignment on the prostate). Dose distributions in organs at risk (OARs) were evaluated using mean dose, generalized equivalent uniform doses (gEUDs) and normal tissue complication probabilities (NTCPs), while mean dose and the volume receiving 98% of the dose (V98%) were used for the targets. RESULTS: PT significantly reduced the mean dose to the OARs and a correlation was seen in the pCTs between the prostate PTV overlapping the relevant OAR and OAR NTCPs, as was also the case for the VMAT plans. The best prostate target coverage across the rCTs for the IMPT plans were seen with two lateral opposed beams, although a poor coverage of the lymph node target was apparent based on V98% compared to the VMAT plans. CONCLUSIONS: PT reduced the mean dose to normal tissues in the irradiation of pelvic lymph nodes and a strong association between the volume overlap and NTCPs in the pCTs were found.
[Mh] Termos MeSH primário: Modelos Biológicos
Movimentos dos Órgãos/efeitos da radiação
Órgãos em Risco/efeitos da radiação
Fótons
Neoplasias da Próstata/radioterapia
Terapia com Prótons
Planejamento da Radioterapia Assistida por Computador/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Masculino
Pelve/efeitos da radiação
Dosagem Radioterapêutica
Reto/efeitos da radiação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1080/0284186X.2017.1317107


  6 / 10742 MEDLINE  
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[PMID]:28462956
[Au] Autor:Boles GC; Owen CJ; Berden G; Oomens J; Armentrout PB
[Ad] Endereço:Department of Chemistry, University of Utah, 315 S. 1400 E. Rm. 2020, Salt Lake City, Utah 84112, USA. armentrout@chem.utah.edu.
[Ti] Título:Experimental and theoretical investigations of infrared multiple photon dissociation spectra of glutamic acid complexes with Zn and Cd .
[So] Source:Phys Chem Chem Phys;19(19):12394-12406, 2017 May 21.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Complexes of glutamic acid (Glu) cationized with Zn and Cd were examined by infrared multiple photon dissociation (IRMPD) action spectroscopy using light generated from a free electron laser. Complexes of deprotonated Glu with Zn , [Zn(Glu-H)ACN] (where ACN = acetonitrile, CH CN), and intact Glu with CdCl , CdCl (Glu) were formed. Notably, photodissociation induces Glu fragmentation rather than ACN loss in the Zn complex. In order to identify the structures formed experimentally, the experimentally obtained spectra were compared to those calculated from optimized structures at the B3LYP/6-311+G(d,p) level for [Zn(Glu-H)ACN] and B3LYP/def2-TZVP level with an SDD effective core potential on cadmium for the CdCl (Glu) system. The main binding motif observed for the heavy metal complex is a charge solvated, tridentate [N,CO ,CO] structure where the metal binds to the backbone amino group and carbonyl oxygens of the side-chain and backbone carboxylic acid groups. The Zn system similarly prefers a [N,CO ,CO ] binding motif, where binding is observed at one oxygen of the backbone carboxylate site along with the backbone amino and side-chain carbonyl groups. In both cases, the theoretically determined lowest-energy conformers explain the experimental [Zn(Glu-H)ACN] and CdCl (Glu) spectra well.
[Mh] Termos MeSH primário: Cádmio/química
Ácido Glutâmico/química
Fótons
Espectrofotometria Infravermelho
Zinco/química
[Mh] Termos MeSH secundário: Complexos de Coordenação/química
Modelos Químicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coordination Complexes); 00BH33GNGH (Cadmium); 3KX376GY7L (Glutamic Acid); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp01786c


  7 / 10742 MEDLINE  
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[PMID]:29195356
[Au] Autor:Wang J; Liu F; Mo Y; Wang Z; Zhang S; Zhang X
[Ad] Endereço:State Key Laboratory of Low-Dimensional Quantum Physics, Department of Physics, Tsinghua University, Beijing 100084, China.
[Ti] Título:A new instrument of VUV laser desorption/ionization mass spectrometry imaging with micrometer spatial resolution and low level of molecular fragmentation.
[So] Source:Rev Sci Instrum;88(11):114102, 2017 Nov.
[Is] ISSN:1089-7623
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mass spectrometry imaging (MSI) has important applications in material research, biology, and medicine. The MSI method based on UV laser desorption/ionization (UVLDI) can obtain images of intact samples, but has a high level of molecular fragmentation. In this work, we report a new MSI instrument that uses a VUV laser (125.3 nm) as a desorption/ionization source to exploit its advantages of high single photon energy and small focus size. The new instrument was tested by the mass spectra of Nile red and FGB (Fibrinogen beta chain) samples and mass spectrometric images of a fly brain section. For the tested samples, the VUVDI method offers lower levels of molecular fragmentations and higher sensitivities than those of the UVLDI method and second ion mass spectrometry imaging method using a Bi beam. The ablation crater produced by the focused VUV laser on a quartz plate has an area of 10 µm . The VUV laser is prepared based on the four-wave mixing method using three collimated laser beams and a heated Hg cell.
[Mh] Termos MeSH primário: Lasers
Espectrometria de Massas/instrumentação
Fótons
[Mh] Termos MeSH secundário: Peso Molecular
Fenômenos Físicos
Espectrofotometria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE
[do] DOI:10.1063/1.4994173


  8 / 10742 MEDLINE  
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[PMID]:29402000
[Au] Autor:Rose M; Senkbeil T; von Gundlach AR; Stuhr S; Rumancev C; Dzhigaev D; Besedin I; Skopintsev P; Loetgering L; Viefhaus J; Rosenhahn A; Vartanyants IA
[Ti] Título:Quantitative ptychographic bio-imaging in the water window.
[So] Source:Opt Express;26(2):1237-1254, 2018 Jan 22.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·10 . As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·10 Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells.
[Mh] Termos MeSH primário: Algoritmos
Fibroblastos
Microscopia/métodos
Intensificação de Imagem Radiográfica/métodos
Difração de Raios X
[Mh] Termos MeSH secundário: Fótons
Dose de Radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.001237


  9 / 10742 MEDLINE  
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[PMID]:29401861
[Au] Autor:Grant RT; Jayaprakash R; Coles DM; Musser A; De Liberato S; Samuel IDW; Turnbull GA; Clark J; Lidzey DG
[Ti] Título:Strong coupling in a microcavity containing ß-carotene.
[So] Source:Opt Express;26(3):3320-3327, 2018 Feb 05.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have fabricated an open-cavity microcavity structure containing a thin film of the biologically-derived molecule ß-carotene. We show that the ß-carotene absorption can be described in terms of a series of Lorentzian functions that approximate the 0-0, 0-1, 0-2, 0-3 and 0-4 electronic and vibronic transitions. On placing this molecular material into a microcavity, we obtain anti-crossing between the cavity mode and the 0-1 vibronic transition, however other electronic and vibronic transitions remain in the intermediate or weak-coupling regime due to their lower oscillator strength and broader linewidth. We discuss the consequences of strong-coupling for the possible modification of photosynthetic processes, or a re-ordering of allowed and optically-forbidden states.
[Mh] Termos MeSH primário: Transferência de Energia
Corantes Fluorescentes
Fotossíntese
Semicondutores
beta Caroteno
[Mh] Termos MeSH secundário: Fótons
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 01YAE03M7J (beta Carotene)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.003320


  10 / 10742 MEDLINE  
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[PMID]:27779273
[Au] Autor:Zhang P; Phipps ME; Goodwin PM; Werner JH
[Ad] Endereço:Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130-4899, United States.
[Ti] Título:Light-sheet microscopy by confocal line scanning of dual-Bessel beams.
[So] Source:J Biomed Opt;21(10):100502, 2016 10 01.
[Is] ISSN:1560-2281
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have developed a light-sheet microscope that uses confocal scanning of dual-Bessel beams for illumination. A digital micromirror device (DMD) is placed in the intermediate image plane of the objective used to collect fluorescence and is programmed with two lines of pixels in the "on" state such that the DMD functions as a spatial filter to reject the out-of-focus background generated by the side-lobes of the Bessel beams. The optical sectioning and out-of-focus background rejection capabilities of this microscope were demonstrated by imaging of fluorescently stained actin in human A431 cells. The dual-Bessel beam system enables twice as many photons to be detected per imaging scan, which is useful for low light applications (e.g., single-molecule localization) or imaging at high speed with a superior signal to noise. While demonstrated for two Bessel beams, this approach is scalable to a larger number of beams.
[Mh] Termos MeSH primário: Imagem Tridimensional/métodos
Microscopia de Fluorescência/métodos
[Mh] Termos MeSH secundário: Linhagem Celular
Desenho de Equipamento
Histocitoquímica
Seres Humanos
Microscopia Confocal/métodos
Microscopia de Fluorescência/instrumentação
Fótons
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1117/1.JBO.21.10.100502



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