|
[PMID]: | 27902267 |
[Au] Autor: | Amoozegar MA; Khansha J; Mehrshad M; Shahzadeh Fazeli SA; Ramezani M; de la Haba RR; Sánchez-Porro C; Ventosa A |
[Ad] Endereço: | 1​Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran. |
[Ti] Título: | Soortia roseihalophila gen. nov., sp. nov., a new taxon in the order Balneolales isolated from a travertine spring, and description of Soortiaceae fam. nov. |
[So] Source: | Int J Syst Evol Microbiol;67(1):113-120, 2017 Jan. | [Is] ISSN: | 1466-5034 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | A novel Gram-stain-negative, slightly halophilic, motile, curved rod with a horseshoe shape, designated strain Bsw-2bT, was isolated from Badab-Soort travertine spring in Iran. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Bsw-2bT belongs to the order Balneolales, showing 84.6 % sequence similarity to Gracilimonastropica DSM 19535T and 84.4 % and 83.9 % sequence similarity to Gracilimonas rosea CL-KR2T and Balneola vulgaris DSM 17893T, respectively. In addition, phenotypic and physiological features could clearly differentiate strain Bsw-2bT from species of the most closely related genera, Gracilimonas, Balneola, Aliifodinibius and Fodinibius. The strain was able to grow with 1-3 % (w/v) (optimum at 2 %) NaCl, at temperatures of 28-34 °C (optimum at 30 °C) and between pH 6.0 and 8.0 (optimum at pH 7.0). The major cellular fatty acids of strain Bsw-2bT were iso-C15 : 0, iso-C13 : 0 and iso-C14 : 0. The polar lipid profile of strain Bsw-2bT was composed predominantly of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and four unknown phospholipids. The DNA G+C content was 40.5 mol%. Based on the evidence from the polyphasic study, strain Bsw-2bT represents a novel species in a novel genus within a new family, for which the name Soortia roseihalophila gen. nov., sp. nov. is proposed, within the new family Soortiaceae fam. nov. The type strain is strain Bsw-2bT (=IBRC-M 10915T=LMG 28547T). |
[Mh] Termos MeSH primário: |
Bacteroidetes/classificação Nascentes Naturais/microbiologia Filogenia
|
[Mh] Termos MeSH secundário: |
Técnicas de Tipagem Bacteriana Bacteroidetes/genética Bacteroidetes/isolamento & purificação Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Irã (Geográfico) Fosfolipídeos/química RNA Ribossômico 16S/genética Análise de Sequência de DNA
|
[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S) |
[Em] Mês de entrada: | 1708 |
[Cu] Atualização por classe: | 170818 |
[Lr] Data última revisão:
| 170818 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161201 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/ijsem.0.001583 |
|
|
|