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[PMID]: | 28853682 |
[Au] Autor: | Khan IU; Habib N; Hussain F; Xian WD; Amin A; Zhou EM; Ahmed I; Zhi XY; Li WJ |
[Ad] Endereço: | 1​Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China. |
[Ti] Título: | Thermus caldifontis sp. nov., a thermophilic bacterium isolated from a hot spring. |
[So] Source: | Int J Syst Evol Microbiol;67(8):2868-2872, 2017 Aug. | [Is] ISSN: | 1466-5034 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | A thermophilic bacterial strain, designated YIM 73026T was isolated from a sediment sample collected from a hot spring in Tibet, PR China. The taxonomic position of the novel isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, aerobic and rod-shaped. Colonies were circular, convex, opaque and yellow. The strain grew at 50-70 °C (optimum, 60 °C), pH 6.0-8.0 (optimum, pH 7.0) and in the presence of up to 1.0 % NaCl (w/v). Comparison of the 16S rRNA gene sequences of YIM 73026T and those of other members of the genus Thermus showed sequence similarities ranging from 91.2 to 97.5 %, with YIM 73026T showing closest sequence similarity to Thermus scotoductus SE-1T (97.5 %). DNA-DNA hybridization results, however, revealed that DNA-DNA reassociation values between YIM 73026T and T. scotoductus DSM 8553T (37.6 %), Thermusamyloliquefaciens YIM 77409T (34.5 %), Thermusantranikianii DSM 12462T (30.3 %), Thermuscaliditerrae YIM 77925T (28.6 %) and Thermustengchongensis YIM 77924T (27.3 %) were well below the 70 % limit for species identification. YIM 73026T contained MK-8 as the respiratory quinone, and iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C15 : 0 as the major cellular fatty acids (>10 %). The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. The genomic DNA G+C content of YIM 73026T was 65.4 mol%. On the basis of morphological, chemotaxonomic and genotypic characteristics, it is proposed that the isolate represents a novel species of the genus Thermus, for which the name Thermus caldifontis sp. nov. is proposed. The type strain is YIM 73026T (=NBRC 112415T=CCTCC AB 2016305T). |
[Mh] Termos MeSH primário: |
Fontes Termais/microbiologia Filogenia Thermus/classificação
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[Mh] Termos MeSH secundário: |
Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácidos Graxos/química Glicolipídeos/química Hibridização de Ácido Nucleico Fosfolipídeos/química RNA Ribossômico 16S/genética Análise de Sequência de DNA Thermus/genética Thermus/isolamento & purificação Tibet Vitamina K 2/análogos & derivados Vitamina K 2/química
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-38-6 (vitamin MK 8) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170918 |
[Lr] Data última revisão:
| 170918 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170831 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/ijsem.0.002037 |
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