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[PMID]: | 28916375 |
[Au] Autor: | Brouchkov A; Kabilov M; Filippova S; Baturina O; Rogov V; Galchenko V; Mulyukin A; Fursova O; Pogorelko G |
[Ad] Endereço: | Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russia; Tyumen State University, Volodarskogo 6, Tyumen 625003, Russia. |
[Ti] Título: | Bacterial community in ancient permafrost alluvium at the Mammoth Mountain (Eastern Siberia). |
[So] Source: | Gene;636:48-53, 2017 Dec 15. | [Is] ISSN: | 1879-0038 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Permanently frozen (approx. 3.5Ma) alluvial Neogene sediments exposed in the Aldan river valley at the Mammoth Mountain (Eastern Siberia) are unique, ancient, and poorly studied permafrost environments. So far, the structure of the indigenous bacterial community has remained unknown. Use of 16S metagenomic analysis with total DNA isolation using DNA Spin Kit for Soil (MO-Bio) and QIAamp DNA Stool Mini Kit (Qiagen) has revealed the major and minor bacterial lineages in the permafrost alluvium sediments. In sum, 61 Operational Taxonomic Units (OTUs) with 31,239 reads (Qiagen kit) and 15,404 reads (Mo-Bio kit) could be assigned to the known taxa. Only three phyla, Bacteroidetes, Proteobacteria and Firmicutes, comprised >5% of the OTUs abundance and accounted for 99% of the total reads. OTUs pertaining to the top families (Chitinophagaceae, Caulobacteraceae, Sphingomonadaceae, Bradyrhizobiaceae, Halomonadaceae) held >90% of reads. The abundance of Actinobacteria was less (0.7%), whereas members of other phyla (Deinococcus-Thermus, Cyanobacteria/Chloroplast, Fusobacteria, and Acidobacteria) constituted a minor fraction of reads. The bacterial community in the studied ancient alluvium differs from other permafrost sediments, mainly by predominance of Bacteroidetes (>52%). The diversity of this preserved bacterial community has the potential to cause effects unknown if prompted to thaw and spread with changing climate. Therefore, this study elicits further reason to study how reintroduction of these ancient bacteria could affect the surrounding ecosystem, including current bacterial species. |
[Mh] Termos MeSH primário: |
Bactérias/isolamento & purificação Pergelissolo/microbiologia
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[Mh] Termos MeSH secundário: |
Bactérias/classificação Bactérias/genética Sedimentos Geológicos/química Sedimentos Geológicos/microbiologia Metagenômica RNA Ribossômico 16S/genética Sibéria
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (RNA, Ribosomal, 16S) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171018 |
[Lr] Data última revisão:
| 171018 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170917 |
[St] Status: | MEDLINE |
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