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[PMID]:29367483
[Au] Autor:Matsuura T; Ogawa A; Ohara Y; Nishina S; Nakanishi M; Gohtani S
[Ad] Endereço:Department of Applied Biological Science, Kagawa University.
[Ti] Título:Effect of Alcohols on the Phase Behavior and Emulsification of a Sucrose Fatty Acid Ester/Water/Edible Oil System.
[So] Source:J Oleo Sci;67(2):167-176, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The effect of alcohols (ethanol, 1-propanol, propylene glycol, glycerin, sucrose) on the phase behavior and emulsification of sucrose stearic acid ester (SSE)/water/edible vegetable oil (EVO) systems was investigated. Adding sucrose, propylene glycol, and glycerin narrowed the oil-separated two-phase region in the phase diagram of the SSE/water/EVO systems, whereas adding ethanol and 1-propanol expanded the oil-separated two-phase region. Changing the course of emulsification in the phase diagram showed that the size of the oil-droplet particle typically decreased in a system with a narrowed oil-separated region. The emulsification properties of the systems varied with respect to changes in the phase diagram. The microstructure of the systems was examined using small-angle X-ray scattering, and the ability to retain the oil in the lamellar structure of the SSEs was suggested as an important role in emulsification, because the mechanism of the systems was the same as that for the liquid crystal emulsification method.
[Mh] Termos MeSH primário: Álcoois/química
Ésteres/química
Ácidos Graxos/química
Transição de Fase
Óleos Vegetais/química
Ácidos Esteáricos/química
Sacarose/química
Água/química
[Mh] Termos MeSH secundário: Emulsões
Glicerol/química
Propilenoglicol/química
Espalhamento a Baixo Ângulo
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Emulsions); 0 (Esters); 0 (Fatty Acids); 0 (Plant Oils); 0 (Stearic Acids); 059QF0KO0R (Water); 4ELV7Z65AP (stearic acid); 57-50-1 (Sucrose); 6DC9Q167V3 (Propylene Glycol); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17146


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[PMID]:29412223
[Au] Autor:Genari B; Leitune VCB; Jornada DS; Aldrigui BR; Pohlmann AR; Guterres SS; Samuel SMW; Collares FM
[Ad] Endereço:Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Dental Materials Laboratory, Porto Alegre, RS, Brazil.
[Ti] Título:Effect on adhesion of a nanocapsules-loaded adhesive system.
[So] Source:Braz Oral Res;32:e008, 2018 Feb 01.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:This study aimed to evaluate the in situ degree of conversion, contact angle, and immediate and long-term bond strengths of a commercial primer and an experimental adhesive containing indomethacin- and triclosan-loaded nanocapsules (NCs). The indomethacin- and triclosan-loaded NCs, which promote anti-inflammatory and antibacterial effects through controlled release, were incorporated into the primer at a concentration of 2% and in the adhesive at concentrations of 1, 2, 5, and 10%. The in situ degree of conversion (DC, n=3) was evaluated by micro-Raman spectroscopy. The contact angle of the primer and adhesive on the dentin surface (n = 3) was determined by an optical tensiometer. For the microtensile bond strength µTBS test (12 teeth per group), stick-shaped specimens were tested under tensile stress immediately after preparation and after storage in water for 1 year. The data were analyzed using two-way ANOVA, three-way ANOVA and Tukey's post hoc tests with α=0.05. The use of the NC-loaded adhesive resulted in a higher in situ degree of conversion. The DC values varied from 75.07 ± 8.83% to 96.18 ± 0.87%. The use of NCs in only the adhesive up to a concentration of 5% had no influence on the bond strength. The contact angle of the primer remained the same with and without NCs. The use of both the primer and adhesive with NCs (for all concentrations) resulted in a higher contact angle of the adhesive. The longitudinal µTBS was inversely proportional to the concentration of NCs in the adhesive system, exhibiting decreasing values for the groups with primer containing NCs and adhesives with increasing concentrations of NCs. Adhesives containing up to 5% of nanocapsules and primer with no NCs maintained the in situ degree of conversion, contact angle, and immediate and long-term bond strengths. Therefore, the NC-loaded adhesive can be an alternative method for combining the bond performance and therapeutic effects. The use of an adhesive with up to 5% nanocapsules containing indomethacin and triclosan and a primer with no nanocapsules maintained the long-term bond performance.
[Mh] Termos MeSH primário: Colagem Dentária/métodos
Indometacina/química
Nanocápsulas/química
Cimentos de Resina/química
Triclosan/química
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Bovinos
Falha de Restauração Dentária
Dentina/efeitos dos fármacos
Teste de Materiais
Transição de Fase/efeitos dos fármacos
Polimerização/efeitos dos fármacos
Valores de Referência
Reprodutibilidade dos Testes
Análise Espectral Raman
Propriedades de Superfície/efeitos dos fármacos
Resistência à Tração
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nanocapsules); 0 (Resin Cements); 4NM5039Y5X (Triclosan); 90881-69-9 (Scotchbond); XXE1CET956 (Indomethacin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


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[PMID]:29416037
[Au] Autor:Zabara A; Chong JTY; Martiel I; Stark L; Cromer BA; Speziale C; Drummond CJ; Mezzenga R
[Ad] Endereço:Department of Health Sciences and Technology, ETH Zurich, Schmelzbergstrasse 9 LFO E23, 8092, Zürich, Switzerland.
[Ti] Título:Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains.
[So] Source:Nat Commun;9(1):544, 2018 02 07.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In meso crystallization of membrane proteins from lipidic mesophases is central to protein structural biology but limited to membrane proteins with small extracellular domains (ECDs), comparable to the water channels (3-5 nm) of the mesophase. Here we present a strategy expanding the scope of in meso crystallization to membrane proteins with very large ECDs. We combine monoacylglycerols and phospholipids to design thermodynamically stable ultra-swollen bicontinuous cubic phases of double-gyroid (Ia3d), double-diamond (Pn3m), and double-primitive (Im3m) space groups, with water channels five times larger than traditional lipidic mesophases, and showing re-entrant behavior upon increasing hydration, of sequences Ia3d→Pn3m→Ia3d and Pn3m→Im3m→Pn3m, unknown in lipid self-assembly. We use these mesophases to crystallize membrane proteins with ECDs inaccessible to conventional in meso crystallization, demonstrating the methodology on the Gloeobacter ligand-gated ion channel (GLIC) protein, and show substantial modulation of packing, molecular contacts and activation state of the ensued proteins crystals, illuminating a general strategy in protein structural biology.
[Mh] Termos MeSH primário: Membrana Celular
Proteínas de Membrana/química
Fosfatidilgliceróis/química
[Mh] Termos MeSH secundário: Cristalização/métodos
Ácidos Graxos Monoinsaturados/química
Canais Iônicos
Transição de Fase
Domínios Proteicos
Termodinâmica
Água
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids, Monounsaturated); 0 (Ion Channels); 0 (Membrane Proteins); 0 (Phosphatidylglycerols); 059QF0KO0R (Water); 4271ZA8WXO (distearoyl phosphatidylglycerol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180209
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-018-02996-5


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[PMID]:29335413
[Au] Autor:Abberley JP; Killah R; Walker R; Storey JMD; Imrie CT; Salamonczyk M; Zhu C; Gorecka E; Pociecha D
[Ad] Endereço:Department of Chemistry, King's College, University of Aberdeen, Aberdeen, AB24 3UE, UK.
[Ti] Título:Heliconical smectic phases formed by achiral molecules.
[So] Source:Nat Commun;9(1):228, 2018 01 15.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chiral symmetry breaking in soft matter is a hot topic of current research. Recently, such a phenomenon was found in a fluidic phase showing orientational order of molecules-the nematic phase; although built of achiral molecules, the phase can exhibit structural chirality-average molecular direction follows a short-pitch helix. Here, we report a series of achiral asymmetric dimers with an odd number of atoms in the spacer, which form twisted structures in nematic as well as in lamellar phases. The tight pitch heliconical nematic (N ) phase and heliconical tilted smectic C (SmC ) phase are formed. The formation of a variety of helical structures is accompanied by a gradual freezing of molecular rotation. In the lowest temperature smectic phase, HexI, the twist is expressed through the formation of hierarchical structure: nanoscale helices and mesoscopic helical filaments. The short-pitch helical structure in the smectic phases is confirmed by resonant X-ray measurements.
[Mh] Termos MeSH primário: Cristais Líquidos/química
Conformação Molecular
Nanoestruturas/química
Transição de Fase
[Mh] Termos MeSH secundário: Dicroísmo Circular
Isomerismo
Microscopia de Força Atômica
Modelos Químicos
Modelos Moleculares
Estrutura Molecular
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02626-6


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[PMID]:29173136
[Au] Autor:Bai T; Chen Y; Jiang W; Yan F; Fan Y
[Ad] Endereço:1 Department of Applied Mechanics, Sichuan University, Chengdu, China.
[Ti] Título:Studies on Foam Decay Trend and Influence of Temperature Jump on Foam Stability in Sclerotherapy.
[So] Source:Vasc Endovascular Surg;52(2):98-106, 2018 Feb.
[Is] ISSN:1938-9116
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: This study investigated the influence of temperature jump and liquid-gas ratio on foam stability to derive the foam-decay law. METHODS: The experimental group conditions were as follows: mutation temperatures (10°C, 16°C, 20°C, 23°C, 25°C, and 27°C to >37°C) and liquid-gas ratios (1:1, 1:2, 1:3, and 1:4). The control group conditions were as follows: temperatures (10°C, 16°C, 20°C, 23°C, 25°C and 27°C) and liquid-gas ratios (1:1, 1:2, 1:3, and 1:4). A homemade device manufactured using the Tessari DSS method was used to prepare the foam. The decay process was videotape recorded. In the drainage rate curve, the temperature rose, and the liquid-gas ratio varied from 1:1 to 1:4, causing faster decay. RESULTS: In the entire process, the foam volume decreased with increasing drainage rate. The relationships were almost linear. Comparison of the experimental and control groups shows that the temperature jump results in a drainage time range of 1 to 15 seconds. The half-life ranges from 10 to 30 seconds. The maximum rate is 18.85%. Changes in the preparation temperature yields a drainage time range of 3 to 30 seconds. The half-life varies from 20 to 60 seconds. CONCLUSION: Decreasing the temperature jump range and liquid-gas ratio gradually enhances the foam stability. The foam decay time and drainage rate exhibit an exponential function distribution.
[Mh] Termos MeSH primário: Soluções Esclerosantes/química
Escleroterapia/métodos
Morruato de Sódio/química
Temperatura Ambiente
[Mh] Termos MeSH secundário: Estabilidade de Medicamentos
Meia-Vida
Modelos Lineares
Modelos Químicos
Transição de Fase
Fatores de Tempo
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sclerosing Solutions); 8031-09-2 (Sodium Morrhuate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1177/1538574417741786


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[PMID]:29364605
[Au] Autor:Skladnev DA; Mulyukin AL; Filippoval SN; Kulikov EE; Letaroval MA; Yuzbasheva EA; Karnysheva EA; Brushkov AV; Gal'chenko VF
[Ti] Título:[Modeling the Propagation of Microbial Cells and Phage Particles from the Sites of Permafrost Thawing.]
[So] Source:Mikrobiologiia;85(5):580-587, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A method is proposed for integral assessment of the propagation of microbial cells and viral parti- cles during seasonal thawing of relic ice wedge layers. The results of on-site and laboratory investigation car- ried out in the upper part of permafrost exposure at Mamontova Gora (Yakutiya, Russia) are presented. To increase reliability of the results, suspensions of two microbial species and two coliphage species were intro- duced as biomarkers directly on the surface of thaing ice and in the meltwater flow. Each of the four different model biological objects was shown to possess unique parameters of movement in the meltwater flow and is able to move 132 m in 25-35 min with the water flow.
[Mh] Termos MeSH primário: Colífagos/fisiologia
Corynebacterium/fisiologia
Movimento/fisiologia
Pergelissolo/microbiologia
Rios/microbiologia
Yarrowia/fisiologia
[Mh] Termos MeSH secundário: Gelo/análise
Modelos Biológicos
Organismos Geneticamente Modificados
Transição de Fase
Reologia/métodos
Estações do Ano
Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ice)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29295619
[Au] Autor:Song Y; Cheng S; Wang H; Zhu BW; Zhou D; Yang P; Tan M
[Ad] Endereço:School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University , Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China.
[Ti] Título:Variable Temperature Nuclear Magnetic Resonance and Magnetic Resonance Imaging System as a Novel Technique for In Situ Monitoring of Food Phase Transition.
[So] Source:J Agric Food Chem;66(3):740-747, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) system with a 45 mm variable temperature (VT) sample probe (VT-NMR-MRI) was developed as an innovative technique for in situ monitoring of food phase transition. The system was designed to allow for dual deployment in either a freezing (-37 °C) or high temperature (150 °C) environment. The major breakthrough of the developed VT-NMR-MRI system is that it is able to measure the water states simultaneously in situ during food processing. The performance of the VT-NMR-MRI system was evaluated by measuring the phase transition for salmon flesh and hen egg samples. The NMR relaxometry results demonstrated that the freezing point of salmon flesh was -8.08 °C, and the salmon flesh denaturation temperature was 42.16 °C. The protein denaturation of egg was 70.61 °C, and the protein denaturation occurred at 24.12 min. Meanwhile, the use of MRI in phase transition of food was also investigated to gain internal structural information. All these results showed that the VT-NMR-MRI system provided an effective means for in situ monitoring of phase transition in food processing.
[Mh] Termos MeSH primário: Ovos/análise
Imagem por Ressonância Magnética/métodos
Espectroscopia de Ressonância Magnética/métodos
Carne/análise
[Mh] Termos MeSH secundário: Animais
Galinhas
Manipulação de Alimentos
Qualidade dos Alimentos
Transição de Fase
Salmão
Temperatura Ambiente
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04334


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[PMID]:28745649
[Au] Autor:Kisserli A; Tabary T; Cohen JHM; Duret V; Mahmoudi R
[Ad] Endereço:Department of Immunology, Reims University Hospitals, Robert Debré Hospital; Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne.
[Ti] Título:High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment.
[So] Source:J Vis Exp;(125), 2017 Jul 18.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Complement receptor 1 (CR1), a transmembrane glycoprotein that plays a key role in the innate immune system, is expressed on many cell types, but especially on red blood cells (RBCs). As a receptor for the complement components C3b and C4b, CR1 regulates the activation of the complement cascade and promotes the phagocytosis of immune complexes and cellular debris, as well as the amyloid-beta (Aß) peptide in Alzheimer's disease (AD). Several studies have confirmed AD-associated single nucleotide polymorphisms (SNPs), as well as a copy-number variation (CNV) in the CR1 gene. Here, we describe an innovative method for determining the length polymorphism of the CR1 receptor. The receptor includes three domains, called long homologous repeats (LHR)-LHR-A, LHR-C, and LHR-D-and an n domain, LHR-B, where n is an integer between 0 and 3. Using a single pair of specific primers, the genetic material is used to amplify a first fragment of the LHR-B domain (the variant amplicon B) and a second fragment of the LHR-C domain (the invariant amplicon). The variant amplicon B and the invariant amplicon display differences at five nucleotides outside of the hybridization areas of said primers. The numbers of variant amplicons B and of invariant amplicons is deduced using a quantitative tool (high-resolution melting (HRM) curves), and the ratio of the variant amplicon B to the invariant amplicon differs according to the CR1 length polymorphism. This method provides several advantages over the canonical phenotype method, as it does not require fresh material and is cheaper, faster, and therefore applicable to larger populations. Thus, the use of this method should be helpful to better understand the role of CR1 isoforms in the pathogenesis of diseases such as AD.
[Mh] Termos MeSH primário: Doença de Alzheimer/patologia
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos
Receptores de Complemento/genética
[Mh] Termos MeSH secundário: Doença de Alzheimer/genética
DNA/isolamento & purificação
DNA/metabolismo
Suscetibilidade a Doenças
Eritrócitos/metabolismo
Genótipo
Seres Humanos
Transição de Fase
Fenótipo
Polimorfismo de Nucleotídeo Único
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Receptores de Complemento/metabolismo
Software
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protein Isoforms); 0 (Receptors, Complement); 9007-49-2 (DNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/56012


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[PMID]:27770420
[Au] Autor:Harper-Leatherman AS; Wallace JM; Rolison DR
[Ad] Endereço:Chemistry and Biochemistry Department, Fairfield University, 1073 North Benson Road, Fairfield, CT, 06824, USA. aharper@fairfield.edu.
[Ti] Título:Cytochrome c Stabilization and Immobilization in Aerogels.
[So] Source:Methods Mol Biol;1504:149-163, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sol-gel-derived aerogels are three-dimensional, nanoscale materials that combine large surface area with high porosity. These traits make them useful for any rate-critical chemical process, particularly sensing or electrochemical applications, once physical or chemical moieties are incorporated into the gels to add their functionality to the ultraporous scaffold. Incorporating biomolecules into aerogels, other than such rugged species as lipases or cellulose, has been challenging due to the inability of most biomolecules to remain structurally intact within the gels during the necessary supercritical fluid (SCF) processing. However, the heme protein cytochrome c (cyt.c) forms self-organized superstructures around gold (or silver) nanoparticles in buffer that can be encapsulated into wet gels as the sol undergoes gelation. The guest-host wet gel can then be processed to form composite aerogels in which cyt.c retains its characteristic visible absorption. The gold (or silver) nanoparticle-nucleated superstructures protect the majority of the protein from the harsh physicochemical conditions necessary to form an aerogel. The Au~cyt.c superstructures exhibit rapid gas-phase recognition of nitric oxide (NO) within the bioaerogel matrix, as facilitated by the high-quality pore structure of the aerogel, while remaining viable for weeks at room temperature. More recently, careful control of synthetic parameters (e.g., buffer concentration, protein concentration, SCF extraction rate) have allowed for the preparation of cyt.c-silica aerogels, sans nucleating nanoparticles; these bioaerogels also exhibit rapid gas-phase sensing while retaining protein structural stability.
[Mh] Termos MeSH primário: Citocromos c/química
Enzimas Imobilizadas/química
Géis/química
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Animais
Estabilidade Enzimática
Ouro/química
Cavalos
Nanopartículas Metálicas/química
Nanopartículas Metálicas/ultraestrutura
Óxido Nítrico/análise
Transição de Fase
Prata/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Gels); 31C4KY9ESH (Nitric Oxide); 3M4G523W1G (Silver); 7440-57-5 (Gold); 7631-86-9 (Silicon Dioxide); 9007-43-6 (Cytochromes c)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:27770414
[Au] Autor:Johnson GR; Luckarift HR
[Ad] Endereço:Hexpoint Technologies, 503 Maryland Boulevard, Mexico Beach, FL, 32456, USA.
[Ti] Título:Enzyme Stabilization via Bio-Templated Silicification Reactions.
[So] Source:Methods Mol Biol;1504:61-73, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Effective entrapment of enzymes in solid phase materials is critical to their practical application. The entrapment generally stabilizes biological activity compared to soluble molecules and the material simplifies catalyst integration compared to other methods. A silica sol-gel process based upon biological mechanisms of inorganic material formation (biomineralization) supports protein immobilization reactions within minutes. The material has high protein binding capacity and the catalytic activity of the enzyme is retained. We have demonstrated that both oligopeptides and selected proteins will mediate the biomineralization of silica and allow effective co-encapsulation of other proteins present in the reaction mixture. The detailed methods described here provide a simple and effective approach for molecular biologists, biochemists and bioengineers to create stable, solid phase biocatalysts that may be integrated within sensors, synthetic processes, reactive barriers, energy conversion, and other biotechnology concepts.
[Mh] Termos MeSH primário: Butirilcolinesterase/química
Enzimas Imobilizadas/química
Muramidase/química
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Animais
Técnicas Biossensoriais
Biotecnologia
Butirilcolinesterase/metabolismo
Galinhas
Ensaios Enzimáticos/métodos
Enzimas Imobilizadas/metabolismo
Muramidase/metabolismo
Peptídeos/química
Transição de Fase
Sílica Gel/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Peptides); 60650-90-0 (Silica Gel); 7631-86-9 (Silicon Dioxide); EC 3.1.1.8 (Butyrylcholinesterase); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE



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