Base de dados : MEDLINE
Pesquisa : G01.645.500 [Categoria DeCS]
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[PMID]:28450256
[Au] Autor:Dietrich MA; Irnazarow I; Ciereszko A
[Ad] Endereço:Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: m.dietrich@pan.olsztyn.pl.
[Ti] Título:Proteomic identification of seminal plasma proteins related to the freezability of carp semen.
[So] Source:J Proteomics;162:52-61, 2017 Jun 06.
[Is] ISSN:1876-7737
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The variation in sperm freezability among individuals within a fish species is a major factor justifying the identification of useful predictive indicators of cryopreservation success. It is unknown at present whether the protein composition of fish seminal plasma affects sperm freezability. Therefore, the aims of this study were to compare the proteome of carp seminal plasma from semen rated as good (GF) and poor (PF) freezability by two-dimensional difference gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF and PF based on sperm motility assessment after freeze/thawing. Five spots representing three proteins were more abundant in GF, while ten spots representing seven proteins were more abundant in PF seminal plasma. The majority of proteins present in higher abundance in PF seminal plasma were associated with the innate immune response. On the other hand, higher freezability was associated with proteins involved in the maintenance of sperm membrane integrity and antioxidative protection. These results indicate that carp semen freezability levels may be related to different seminal plasma protein profiles. Lower usefulness of spermatozoa in cryopreservation may be related to previous infection or stress leading to sublethal changes to sperm structure. SIGNIFICANCE: Sperm quality parameters such as motility, viability and sperm concentration have been used as predictive tools of sperm cryopreservation potential in fish species However, the usefulness of initial motility parameters as indicators of freezability varies among fish species and between individuals within a species. Recent studies in mammals revealed that male-to-male variability in cryoresistance can be attributed to differences in seminal plasma protein composition. To the best of our knowledge, no proteomic studies linking the protein composition of fish seminal plasma and freezing resilience have been performed in fish. Our results indicate for the first time that factors regulating how carp semen tolerate cryopreservation may be related to the different protein profiles of carp seminal plasma. The obtained results provide new insight into understanding the molecular mechanisms underlying cryoresistance of carp semen and provide a tool for the improvement of a long-term sperm preservation procedure.
[Mh] Termos MeSH primário: Carpas
Congelamento
Sêmen/química
Proteínas de Plasma Seminal/análise
[Mh] Termos MeSH secundário: Animais
Criopreservação
Masculino
Proteômica/métodos
Sêmen/citologia
Espermatozoides/citologia
Espectrometria de Massas em Tandem
Eletroforese em Gel Diferencial Bidimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Seminal Plasma Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:29430905
[Au] Autor:Sbitnev AV; Vodianova MA; Kriatov IA; Donerian LG; Evseeva IS; Ushakova OV; Ushakov DI; Matveeva IS; Rodionova OM
[Ti] Título:[Methodological aspects of the assessment of phytotoxicic properties of ice-melter reagents].
[So] Source:Gig Sanit;95(8):773-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:One of the main criteria which determine the possibility of the use of a particular type of ice-melter reagents (IMR) is the degree of their safety for the environment and human health, which is reflected in the establishment of safe doses and concentrations. In this regard, the current area of research is to improve the ecological and epidemiological principles of risk assessment of modern types of anti-icing agents. Currently available data concerning monitoring soil studies and the snow held in various cities of Russia, show that there is a process of accumulation of the main components of IMR - sodium and chlorine ions in the areas related to the roadway. The article is designated a problem of existing methodological approaches to the assessment of the phytotoxic impact in the investigation of anti-icing agents in the laboratory. There was executed the comparative characteristics of the results of the preliminary pilot studies on the phytotoxic properties of IMR under using different substrates for germination of seeds - soil and filter paper. The data obtained are characterized by differences in the degree of phytotoxic action of the same species depending upon ice-melter reagents methodical setting circuit laboratory experiment. As a result, there was shown the imperfection of the existing method of rapid analysis in relation to ice-melter materials (IMM).
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Congelamento
Gelo
Plantas/efeitos dos fármacos
Neve
Cloreto de Sódio
Poluentes do Solo
[Mh] Termos MeSH secundário: Poluição Ambiental/efeitos adversos
Poluição Ambiental/análise
Poluição Ambiental/prevenção & controle
Seres Humanos
Federação Russa
Cloreto de Sódio/química
Cloreto de Sódio/toxicidade
Poluentes do Solo/química
Poluentes do Solo/toxicidade
Solventes/química
Fenômenos Toxicológicos
Transportes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ice); 0 (Soil Pollutants); 0 (Solvents); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:28742378
[Au] Autor:Arroyo G; Ortiz Barrientos KA; Lange K; Nave F; Miss Mas G; Lam Aguilar P; Soto Galindo MA
[Ad] Endereço:1 Department of Citohistología, Universidad de San Carlos de Guatemala , Guatemala, Guatemala .
[Ti] Título:Effect of the Various Steps in the Processing of Human Milk in the Concentrations of IgA, IgM, and Lactoferrin.
[So] Source:Breastfeed Med;12(7):443-445, 2017 09.
[Is] ISSN:1556-8342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Human milk immune components are unique and important for the development of the newborn. Milk processing at the Human Milk Banks (HMB), however, causes partial destruction of immune proteins. The objective of this study was to determine the effects that heating during the milk processing procedure at the HMB had on the concentrations of IgA, IgM, and lactoferrin at three critical points in time. MATERIALS AND METHODS: Fifty milk samples (150 mL) were collected from voluntary donors at the HMB at the Hospital Nacional Pedro de Bethancourt, located in Antigua Guatemala. Samples from three critical points in time during the milk processing procedure were selected for analysis: freezing/thawing I, freezing/thawing II, and pasteurization. IgA, IgM, and lactoferrin concentrations were determined during each critical point and compared with a baseline concentration. RESULTS: After milk processing, IgA, IgM, and lactoferrin mean concentrations were reduced by 30.0%, 36.0%, and 70.0%, respectively (p < 0.001). Reduction of biological activity was mainly attributed to pasteurization for IgA and lactoferrin (p < 0.001); the first freezing/thawing processes before pasteurization showed no significant reduction difference between mean concentrations of IgA (p = 0.160) and lactoferrin (p = 0.345) but showed a significant effect on IgM concentration (p = 0.016), and the second freezing/thawing procedure only showed a significant effect on IgA (p < 0.001). CONCLUSIONS: The effects of milk processing on the immune proteins that were evaluated in this study demonstrated a significant reduction.
[Mh] Termos MeSH primário: Conservação de Alimentos/métodos
Imunoglobulina A/análise
Imunoglobulina M/análise
Lactoferrina/análise
Bancos de Leite
Leite Humano/química
[Mh] Termos MeSH secundário: Feminino
Congelamento
Seres Humanos
Valor Nutritivo
Pasteurização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immunoglobulin A); 0 (Immunoglobulin M); EC 3.4.21.- (Lactoferrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1089/bfm.2016.0154


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[PMID]:29385390
[Au] Autor:Chen T; Cong Q; Qi Y; Jin J; Choy KL
[Ad] Endereço:Key Laboratory of Bionic Engineering, Ministry of Education, Jilin University, Changchun, Jilin Province, P.R., China.
[Ti] Título:Hydrophobic durability characteristics of butterfly wing surface after freezing cycles towards the design of nature inspired anti-icing surfaces.
[So] Source:PLoS One;13(1):e0188775, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The hydrophobicity and anti-icing performance of the surfaces of some artificial hydrophobic coatings degraded after several icing and de-icing cycles. In this paper, the frost formation on the surfaces of butterfly wings from ten different species was observed, and the contact angles were measured after 0 to 6 frosting/defrosting cycles. The results show that no obvious changes in contact angle for the butterfly wing specimens were not obvious during the frosting/defrosting process. Further, the conclusion was inferred that the topography of the butterfly wing surface forms a special space structure which has a larger space inside that can accommodate more frozen droplets; this behavior prevents destruction of the structure. The findings of this study may provide a basis and new concepts for the design of novel industrially important surfaces to inhibit frost/ice growth, such as durable anti-icing coatings, which may decrease or prevent the socio-economic loss.
[Mh] Termos MeSH primário: Borboletas
Congelamento
Gelo
Asas de Animais
[Mh] Termos MeSH secundário: Animais
Borboletas/classificação
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ice)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188775


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[PMID]:29329343
[Au] Autor:Liu Z; Dugan B; Masiello CA; Wahab LM; Gonnermann HM; Nittrouer JA
[Ad] Endereço:Department of Earth, Environmental, and Planetary Sciences, Rice University, Houston, Texas, United States of America.
[Ti] Título:Effect of freeze-thaw cycling on grain size of biochar.
[So] Source:PLoS One;13(1):e0191246, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biochar may improve soil hydrology by altering soil porosity, density, hydraulic conductivity, and water-holding capacity. These properties are associated with the grain size distributions of both soil and biochar, and therefore may change as biochar weathers. Here we report how freeze-thaw (F-T) cycling impacts the grain size of pine, mesquite, miscanthus, and sewage waste biochars under two drainage conditions: undrained (all biochars) and a gravity-drained experiment (mesquite biochar only). In the undrained experiment plant biochars showed a decrease in median grain size and a change in grain-size distribution consistent with the flaking off of thin layers from the biochar surface. Biochar grain size distribution changed from unimodal to bimodal, with lower peaks and wider distributions. For plant biochars the median grain size decreased by up to 45.8% and the grain aspect ratio increased by up to 22.4% after 20 F-T cycles. F-T cycling did not change the grain size or aspect ratio of sewage waste biochar. We also observed changes in the skeletal density of biochars (maximum increase of 1.3%), envelope density (maximum decrease of 12.2%), and intraporosity (porosity inside particles, maximum increase of 3.2%). In the drained experiment, mesquite biochar exhibited a decrease of median grain size (up to 4.2%) and no change of aspect ratio after 10 F-T cycles. We also document a positive relationship between grain size decrease and initial water content, suggesting that, biochar properties that increase water content, like high intraporosity and pore connectivity large intrapores, and hydrophilicity, combined with undrained conditions and frequent F-T cycles may increase biochar breakdown. The observed changes in biochar particle size and shape can be expected to alter hydrologic properties, and thus may impact both plant growth and the hydrologic cycle.
[Mh] Termos MeSH primário: Carvão Vegetal/química
Solo/química
[Mh] Termos MeSH secundário: Ecossistema
Congelamento
Hidrologia
Tamanho da Partícula
Pinus
Poaceae
Porosidade
Prosopis
Esgotos
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sewage); 0 (Soil); 0 (biochar); 16291-96-6 (Charcoal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191246


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[PMID]:29229335
[Au] Autor:Liu YE; Huang LQ; Luo XJ; Tan XX; Huang CC; Corella PZ; Mai BX
[Ad] Endereço:State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
[Ti] Título:Determination of organophosphorus flame retardants in fish by freezing-lipid precipitation, solid-phase extraction and gas chromatography-mass spectrometry.
[So] Source:J Chromatogr A;1532:68-73, 2018 Jan 12.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An analytical method has been developed for measuring 12 organophosphorus flame retardants (PFRs) in fish tissue samples. After the Soxhlet extraction of PFRs with dichloromethane. The experimental parameters of the clean-up were systematically optimized. Methanol was found to be a more effective solvent than acetonitrile used in freezing-lipid precipitation. Methanol (5%) in ultrapure water, was finally selected to perform solid-phase extraction (SPE, Oasis HLB cartridge), with mean lipid removal efficiency of 94% after freezing-lipid precipitation. Further purification followed by 200mg of Z-Sep and C18 dispersant to eliminate the remaining interferences. Quantification was performed using gas chromatography-mass spectrometry in selective ion monitoring mode. The recovery, precision, and the method detection limits (MDLs) were verified by spiking experiments. All chemicals except triethyl phosphate (TEP) showed satisfactory recoveries in the range of 73-107% and 56-108% in the spiked blanks samples and spiked fish tissue samples, respectively. MDLs for PFRs in the biological samples ranged from 0.004 to 0.059ng/g. The proposed method successfully applied to the determination of PFRs in real fish samples with recoveries of four internal standards varying from 75 to 97%. The results demonstrated that the proposed method is highly effective for analyzing PFRs in fish samples.
[Mh] Termos MeSH primário: Técnicas de Química Analítica/métodos
Peixes
Retardadores de Chama/análise
Congelamento
Cromatografia Gasosa-Espectrometria de Massas
Extração em Fase Sólida
[Mh] Termos MeSH secundário: Animais
Técnicas de Química Analítica/instrumentação
Limite de Detecção
Lipídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flame Retardants); 0 (Lipids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:29293608
[Au] Autor:Wu X; Zhu X; Wang Y; Liu X; Chen L; Duan Y
[Ad] Endereço:College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China.
[Ti] Título:The cold tolerance of the northern root-knot nematode, Meloidogyne hapla.
[So] Source:PLoS One;13(1):e0190531, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The northern root-knot nematode, Meloidogyne hapla, is one of the most important nematode pathogens occurring in cold regions. It is a sedentary, biotrophic parasites of plants and overwinter in the soil or in diseased roots. This study showed that the cold tolerance for the second-stage juveniles (J2) of M. hapla was moderate with the 50% survival temperature (S50) of -2.22°C and the fatal temperature was -6°C when cooling at 0.5°C min-1. Cryoprotective dehydration significantly enhance cold tolerance of M. hapla J2 with the lowest S50 of -3.28°C after held being at -1°C for 6 h. Moreover, cold shock and cold acclimation had significant effects on the freezing survival of M. hapla J2. The lethal temperature of eggs was -18°C. Therefore, the cold tolerance of M. hapla is sufficiently favorable to withstand winters in cold temperature environments.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Temperatura Baixa
Tylenchoidea/fisiologia
[Mh] Termos MeSH secundário: Animais
Congelamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190531


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[PMID]:29381286
[Au] Autor:Guseynova BM
[Ti] Título:[Nutrition value of wild-growing fruits from mountain Dagestan and its safety after fast freezing and cold storage].
[So] Source:Vopr Pitan;85(4):76-81, 2016.
[Is] ISSN:0042-8833
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Results of research of mineral composition, content of vitamin C and P, titrable acids, pectinaceous and phenol substances in fresh, fast-frozen (t=-30 °C), and also stored within 3 and 9 months (t=-18 °C) fruits of wild-growing blackberry, cornel, medlar and sea-buckthorn are presented in article. Determination of mineral composition was carried out by flame atomic absorption photometry, vitamin C and pectinaceous substances -by titirimetric methods, phenolic substances and vitamin P - by colorimetric methods. Vitamin C content was minimal in fresh fruits of cornel (6.9±0.3 mg%), amounted to 21.7-32.0 mg% in the fruits of blackberries and medlar and reached 180.1±7.2 mg% in the fruit of sea-buckthorn. Vitamin P concentration ranged from 34.9 (sea-buckthorn) to 180.0 mg% (cornel). Berries of a cornel contained also the greatest number of titrable acids (33.2±1.3 g/dm3), phenolic compounds (243.0±4.8 mg%) and pectinaceous substances (2.91±0.08%). The most significant content of potassium (521±15.6 mg%), calcium (133.2±5.2 mg%), magnesium (62.4±2.5 mg%) and iron (2.81±0.05 mg%) was revealed in medlar fruits. Consumption of 100 g of the studied fruits provides daily requirements of a human body, depending on a species of wild plants: in calcium -for 2-13.3%, potassium - for 7.0-20.8%, magnesium - for 8.1-15.6%, iron - for 5.9-19.2% and in vitamin C - from 5.8-24.6 to 145.7% in the case of sea buckthorn. The applied processing method of conservation - fast freezing (t=-30 °Ð¡) of fruits and their long storage (t=-18 °Ð¡) is the effective way ensuring high safety of nutrients in them. In the studied berries after 9-months cold storage the safety of vitamin C varied ranging from 55.7 (blackberry) to 76.1% (cornel), and vitamin P - from 81.9 (sea-buckthorn) to 92.8% (cornel). Stability of titrable acids, except for medlar fruits, varied from 84.2% (blackberry) to 94.0% (sea-buckthorn). The safety of phenolic and pectinaceous compounds by the end of 9 months of storage, has averaged 90.6 and 95.6% respectively in comparison with their initial quantity in fresh fruits. The mineral composition was the stablest. After completion of experiment the safety of mineral substances in fruits of wild plants fluctuated from 94.6 to 98.5%. Distinctions in change of biochemical complexes of berries of blackberry, cornel, medlar and sea-buckthorn at fast freezing (t=-30 °Ð¡) and storage (t=-18 °Ð¡), apparently, are caused by specific features, content of free and bound water, thickness of cellular walls, durability of a thin skin of fruits, as well as by concentration of the components that inhibit the destructive processes occurring at the cellular and molecular level.
[Mh] Termos MeSH primário: Análise de Alimentos
Conservação de Alimentos
Inocuidade dos Alimentos
Congelamento
Frutas/química
Valor Nutritivo
[Mh] Termos MeSH secundário: Daguestão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE


  9 / 21338 MEDLINE  
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[PMID]:28668640
[Au] Autor:Mak PJ; Denisov IG
[Ad] Endereço:Department of Chemistry, Saint Louis University, St. Louis, MO, United States. Electronic address: makp@slu.edu.
[Ti] Título:Spectroscopic studies of the cytochrome P450 reaction mechanisms.
[So] Source:Biochim Biophys Acta;1866(1):178-204, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The cytochrome P450 monooxygenases (P450s) are thiolate heme proteins that can, often under physiological conditions, catalyze many distinct oxidative transformations on a wide variety of molecules, including relatively simple alkanes or fatty acids, as well as more complex compounds such as steroids and exogenous pollutants. They perform such impressive chemistry utilizing a sophisticated catalytic cycle that involves a series of consecutive chemical transformations of heme prosthetic group. Each of these steps provides a unique spectral signature that reflects changes in oxidation or spin states, deformation of the porphyrin ring or alteration of dioxygen moieties. For a long time, the focus of cytochrome P450 research was to understand the underlying reaction mechanism of each enzymatic step, with the biggest challenge being identification and characterization of the powerful oxidizing intermediates. Spectroscopic methods, such as electronic absorption (UV-Vis), electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), electron nuclear double resonance (ENDOR), Mössbauer, X-ray absorption (XAS), and resonance Raman (rR), have been useful tools in providing multifaceted and detailed mechanistic insights into the biophysics and biochemistry of these fascinating enzymes. The combination of spectroscopic techniques with novel approaches, such as cryoreduction and Nanodisc technology, allowed for generation, trapping and characterizing long sought transient intermediates, a task that has been difficult to achieve using other methods. Results obtained from the UV-Vis, rR and EPR spectroscopies are the main focus of this review, while the remaining spectroscopic techniques are briefly summarized. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/química
Heme/química
Ferro/química
Oxigênio/química
[Mh] Termos MeSH secundário: Biocatálise
Espectroscopia de Ressonância de Spin Eletrônica/instrumentação
Espectroscopia de Ressonância de Spin Eletrônica/métodos
Radicais Livres/química
Congelamento
Glicerol/química
Espectroscopia de Ressonância Magnética/instrumentação
Espectroscopia de Ressonância Magnética/métodos
Modelos Moleculares
Oxirredução
Estrutura Secundária de Proteína
Análise Espectral Raman/instrumentação
Análise Espectral Raman/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; REVIEW
[Nm] Nome de substância:
0 (Free Radicals); 42VZT0U6YR (Heme); 9035-51-2 (Cytochrome P-450 Enzyme System); E1UOL152H7 (Iron); PDC6A3C0OX (Glycerol); S88TT14065 (Oxygen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


  10 / 21338 MEDLINE  
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[PMID]:28873601
[Au] Autor:Jenkelunas PJ; Li-Chan ECY
[Ad] Endereço:The University of British Columbia, Faculty of Land and Food Systems, Food Nutrition and Health Program, 2205 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
[Ti] Título:Production and assessment of Pacific hake (Merluccius productus) hydrolysates as cryoprotectants for frozen fish mince.
[So] Source:Food Chem;239:535-543, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate application of fish protein hydrolysates (FPHs) as cryoprotectants for cod fish mince subjected to freeze-thaw abuse. Response surface methodology revealed little difference in cryoprotectant ability between FPHs produced from Pacific hake muscle within the range of conditions studied, namely Flavourzyme® enzyme/substrate ratio (E/S 1-4%), time (1-6h) and pH (5-7). When added at 4% or higher concentrations, FPH minimized expressible moisture and cook loss, while maximizing salt extractable protein from freeze-thaw abused fish mince, providing similar or better cryoprotection compared to an 8% sucrose-sorbitol blend, and a stabilizing effect of FPH on myosin was observed by differential scanning calorimetry. Sensory evaluation showed that addition of 8% FPH in fish ball products increased the perception of fishiness, saltiness, bitterness and firmness while decreasing moistness. FPH could be a viable alternative to the sugar-based cryoprotectants currently used for frozen fish products.
[Mh] Termos MeSH primário: Gadiformes
[Mh] Termos MeSH secundário: Animais
Crioprotetores
Congelamento
Hidrolisados de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryoprotective Agents); 0 (Protein Hydrolysates)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE



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