[PMID]: | 27777629 |
[Au] Autor: | Lau AC; Zhu KP; Brouhard EA; Davis MB; Csankovszki G |
[Ad] Endereço: | Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 N. University Ave., Ann Arbor, MI 48109-1048 USA ; Genome Technologies, The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032 USA. |
[Ti] Título: | An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in males. |
[So] Source: | Epigenetics Chromatin;9:44, 2016. |
[Is] ISSN: | 1756-8935 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: In , in order to equalize gene expression between the sexes and balance X and autosomal expression, two steps are believed to be required. First, an unknown mechanism is hypothesized to upregulate the X chromosome in both sexes. This mechanism balances the X to autosomal expression in males, but creates X overexpression in hermaphrodites. Therefore, to restore the balance, hermaphrodites downregulate gene expression twofold on both X chromosomes. While many studies have focused on X chromosome downregulation, the mechanism of X upregulation is not known. RESULTS: To gain more insight into X upregulation, we studied the effects of chromatin condensation and histone acetylation on gene expression levels in male . We have found that the H4K16 histone acetyltransferase MYS-1/Tip60 mediates dramatic decondensation of the male X chromosome as measured by FISH. However, RNA-seq analysis revealed that MYS-1 contributes only slightly to upregulation of gene expression on the X chromosome. These results suggest that the level of chromosome decondensation does not necessarily correlate with the degree of gene expression change in vivo. Furthermore, the X chromosome is more sensitive to MYS-1-mediated decondensation than the autosomes, despite similar levels of H4K16ac on all chromosomes, as measured by ChIP-seq. H4K16ac levels weakly correlate with gene expression levels on both the X and the autosomes, but highly expressed genes on the X chromosome do not contain exceptionally high levels of H4K16ac. CONCLUSION: These results indicate that H4K16ac and chromosome decondensation influence regulation of the male X chromosome; however, they do not fully account for the high levels of gene expression observed on the X chromosomes. |
[Mh] Termos MeSH primário: |
Proteínas de Caenorhabditis elegans/metabolismo Caenorhabditis elegans/metabolismo Histona Acetiltransferases/metabolismo Cromossomo X/metabolismo
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[Mh] Termos MeSH secundário: |
Acetilação Animais Caenorhabditis elegans/genética Proteínas de Caenorhabditis elegans/genética Cromatina/metabolismo Montagem e Desmontagem da Cromatina Imunoprecipitação da Cromatina Compensação de Dosagem (Genética) Expressão Gênica Histona Acetiltransferases/genética Histonas/metabolismo Hibridização in Situ Fluorescente Masculino Análise de Sequência de DNA Cromossomo X/genética
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL |
[Nm] Nome de substância:
| 0 (Caenorhabditis elegans Proteins); 0 (Chromatin); 0 (Histones); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (MYS-1 protein, C elegans) |
[Em] Mês de entrada: | 1801 |
[Cu] Atualização por classe: | 180310 |
[Lr] Data última revisão:
| 180310 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161026 |
[St] Status: | MEDLINE |
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